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1.
J Hazard Mater ; 384: 121287, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32028548

RESUMO

This study investigated the coupled dynamics of the redox transformation of arsenic-containing ferrihydrite, and arsenate desorption and re-adsorption in presence of sulfide. Batch experiments, various microscopic and spectroscopic analyses collectively revealed that electrons from sulfide competitively transferred to ferrihydrite and no arsenate was reduced. The reductive dissolution of ferrihydrite by sulfide led to the quick formation of FeS that competitively decreased the availability of sulfide for its subsequent reduction of ferrihydrite. The quick formation of FeS was followed by a relatively slow transformation of ferrihydrite to magnetite and other Fe(II)-Fe(III) minerals that were primarily bound to the residual ferrihydrite surfaces. As a result of the preservation of As-containing ferrihydrite and surface covering by the secondary minerals, the majority (> 90%)of sorbed arsenate resided in the solid phase, and <10% of arsenate participated in the desorption process during the ferrihydrite dissolution and transformation. The desorption of arsenate was fast, and followed by the kinetic re-adsorption. The rate and extent of the re-adsorption was consistent with the dynamic transformation of the secondary minerals and their sorption affinity toward As. The results have a strong implication to understanding of As concentration changes during the redox transformation of As-containing minerals in groundwater systems.

2.
Nanotechnology ; 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32059206

RESUMO

A three-step synthesis strategy has been applied to the preparation of Co9S8-loaded tubular carbon nanofibers (CTCNFs/Co9S8 hybrid nanofibers) with excellent microwave absorbing ability. Firstly, tubular polymer nanofibers (TPNFs) are synthesized by our developed confined self-condensation method. Afterwards, TPNFs are converted into surface carboxylated tubular carbon nanofibers (CTCNFs) by carbonization and subsequent acidification processes. Finally, hydrothermal method is used for the controllable growth of Co9S8 nanoparticles on CTCNFs, and a series of CTCNFs/Co9S8 hybrid nanofibers with different Co9S8 loading are obtained. The prepared CTCNFs/Co9S8 hybrid nanofibers possess abundant effective interface and defect dipoles, which will lead to stronger polarization. By the strategy of enhancing dielectric loss, the microwave dissipation ability of CTCNFs/Co9S8 hybrid nanofibers has been significantly improved, showing excellent low-frequency absorbing performance with a minimum reflection loss of -46.81 dB@5.3 GHz. In addition, the composition, structure and properties of nanofibers have been systematically characterized. The Co9S8 loading on CTCNFs and the filler content of CTCNFs/Co9S8 hybrid nanofibers in matrix are studied and optimized. Meanwhile, the microwave attenuation mechanism has been explained.

3.
Nanoscale ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32016197

RESUMO

Poly[3-(4-carboxybutyl)thiophene-2,5-diyl] (P3CT) has been noticed as a promising hole transport layer (HTL) for high-performance inverted planar perovskite solar cells (PSCs) due to its excellent stability and relatively high hole mobility. As we all know, the morphology of perovskite films is largely influenced by the substrate materials. Considering the affinity of alkali metal ions Rb+ and Cs+ with perovskite materials, inverted perovskite solar cells using alkali metal ion (Rb+, Cs+) doped P3CT (denoted as P3CT-Rb and P3CT-Cs) as the HTLs were investigated in this work. It turned out that the work function (WF) of P3CT-Rb matches well with the valence band of perovskites. The perovskite (MAPbI3-xClx) film deposited on top of the P3CT-Rb film exhibited a dense and uniform morphology with superior crystallinity and few pinholes. Consequently, a high efficiency of 20.52% was achieved for P3CT-Rb HTL-based devices, with an impressive open-circuit voltage (Voc) of 1.144 V and a high fill factor (FF) of 82.78%.

4.
BMC Complement Med Ther ; 20(1): 23, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-32020892

RESUMO

BACKGROUND: Chondrocyte apoptosis activated by the mitochondrial dependent pathway serves a crucial role in cartilage degeneration of osteoarthritis (OA). In the present study, the protective effects of CMCS against sodium nitroprusside (SNP)-induced chondrocyte apoptosis were evaluated and the underlying molecular mechanisms were elucidated. METHODS: Chondrocytes were isolated from articular cartilage of SD rats and identified by type II collagen immunohistochemistry. The chondrocytes stimulated with or without SNP to induce apoptosis, were treated by CMCS for various concentrations. The cell viability were determined by MTT and LDH assays. Cell apoptotic ratio was determined by Annexin V-FITC/PI staining. Mitochondrial membrane potential (ΔΨm) was detected by using Rhodamine123 (Rho123) staining. To understand the mechanism, the mRNA expression levels of Bcl-2, Bax, cytochrome c (Cyt c) and cleaved caspase-3 were detected by real-time PCR and western blot analysis, respectively. RESULTS: It was shown using the MTT and LDH assays that CMCS protected the viability of chondrocyte against SNP damage. Annexin V-FITC/PI and Rho123 staining showed that CMCS not only inhibited the cell apoptosis but also restored the reduction of the ΔΨm in chondrocytes. In SNP-induced chondrocytes, CMCS down-regulated the expression of Bax, Cyt c and cleaved caspase-3 but upregulated the expression of Bcl-2, as shown by real-time PCR and western blot. CONCLUSIONS: Taken together, these results indicated that CMCS has the protective effect on chondrocytes against SNP-induced apoptosis, at least partly, via inhibiting the mitochondrial dependent apoptotic pathway. Thus, CMCS may be potentially used as a biological agent for prevention and treatment of OA.

6.
J Med Virol ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32043608

RESUMO

Hepatocellular carcinoma (HCC) accounts for 90% of cases of liver cancer and is one of the most common and lethal malignancies among all cancers. Current screening practices in high-risk populations using ultrasound and serological AFP have significantly reduced HCC mortality. However, considering the highly operative-dependent nature of ultrasound and dissatisfactory diagnostic performance of AFP, there is an unfulfilled need for a biomarker that can be used in HCC-related at-risk population screening. Here, sera from 322 patients, including 105 cases of chronic hepatitis (CH), 116 of liver cirrhosis (LC) and 101 of hepatocellular carcinoma (HCC), were collected. Two biomarkers, Osteopontin (OPN) and Dickkopf WNT signaling pathway inhibitor 1 (DKK1), were evaluated and compared with AFP alone and in combination. In our data, the serum OPN level increased significantly in HCC even in tumors of less than 2 cm. The AUC reached 0.851, much higher than AFP and DKK1, with 79.21% sensitivity and 79.64% specificity at optimal cutoff in all of the samples. In AFP-negative samples, serum OPN also performed well with an AUC of 0.838. The combination of AFP and OPN improved diagnosis performance significantly when compared with AFP alone. However, the DKK1 level showed an increase in HCC only compared with the LC group. The AUC does not improve significantly when added into the binary logistic model. We conclude that OPN, but not DKK1, is a promising biomarker for HCC diagnosis. This article is protected by copyright. All rights reserved.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31940247

RESUMO

Hu-Antigen R (HuR) is involved in the carcinogenesis and progression of multiple types of cancer. However, its precise role in gastric cancer (GC) and the relevant molecular mechanism remain largely unclear. In the present study, we found that HuR expression level was higher in GC tissues and cell lines than in adjacent normal tissues and normal gastric epithelial cell lines, and this elevated expression was found has a significantly association with the lymph node metastasis. Moreover, Silencing HuR with RNA interference inhibited cell viability and induced cell apoptosis through the apoptosis related regulators (Bcl-2 and Bax) in GC cells. Beside, bioinformatic analysis revealed that HuR expression was inversely correlated with miR-145 expression in GC tissue samples and HuR was identified as a direct target of miR-145 with the dual-luciferase reporter. Enforced expression of miR-145 inhibited the HuR expression at both mRNA and protein levels as well as induced a similar biologic effects of silencing HuR in GC cells. Additional, we also found that restoration of HuR could eliminate the effects induced by miR-145 in GC cells. Taken together, these findings demonstrate the exact role of miR-145-HuR axis in the progression of GC and posits a potential target for GC therapy.

8.
Am J Bot ; 107(1): 126-138, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31944266

RESUMO

PREMISE: Apocynaceae is common in the fossil record, especially as seed remains from the Neogene of Europe and North America, but rare in Asia. Intrafamilial assignment is difficult due to the lack of diagnostic characters, and new fossil and modern data are needed to understand the paleobiogeography of this group. METHODS: We studied three Apocynaceae seed impressions from the Lower Eocene Niubao Formation, Jianglang village, Bangor County, central Qinghai-Tibetan Plateau. Morphological data from living and fossil species were phylogenetically mapped to enable systematic assignment. RESULTS: We describe a new genus, Asclepiadospermum gen. nov., and two new species, A. marginatum sp. nov. and A. ellipticum sp. nov. These species are characterized by an elliptical seed, a margin surrounding the central part of the seed, and polygonal, irregular, and small epidermal cells, and differ mainly in terms of the size of the margin and the shape of the apex. All these characters indicate that this new genus belongs to the subfamily Asclepiadoideae (Apocynaceae). CONCLUSIONS: These fossils represent the earliest fossil seed records of Asclepiadoideae. Asclepiadospermum indicates a humid tropical to subtropical flora during the early Eocene in central Tibet. Moreover, our discoveries indicate a close floristic connection between Eurasia and Africa during the early Eocene, which expands our knowledge of the floristic linkage between Tibet and other regions at that time.

9.
Adv Healthc Mater ; : e1901705, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31977157

RESUMO

Synthetic carriers of nucleic acids remain inefficient for practical applications due to their insufficient functions as compared with viral vectors developed by evolution. Here, a synthetic carrier is designed to structurally mimic lentivirus, a widely-used viral vector in therapeutic developments, for its neutral phospholipid membrane tightly anchored on the surface of a packed nucleic acid core. Unlike the reported lipopolyplexes of which the surface membrane around the nucleic acid core is formed from charged lipids, the stable attachment of the neutral lipids to each polyplex core in the present system is achieved through preadsorbed micelles of multicarboxyl amphiphilic molecules as lipid bilayer anchors. The adsorbed micelles are under a tension of deformation due to the electrostatic attraction of the head groups to the cationic surface and their "thermodynamic responsibility" to cover the hydrophobic tails in water. When liposomes of neutral phospholipids approach, the hydrophobic tail groups of the adsorbed micelles may insert into the lipid bilayer matrix to induce them to fuse around polyplex and relieve the thermodynamic tension. The formed neutral phospholipid membrane may encapsulate the polyplex core stably, prevent siRNA from prephagocytic leaking and degrading, and immobilize functional agents with increased capacity.

10.
J Am Chem Soc ; 142(4): 2074-2082, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31909992

RESUMO

Selectivity of electrocatalysts is determined not only by active sites for specific substrate interactions but also by the efficiency of electronic coupling mediated by intervening matrices. Here, we demonstrate the design of electron transport pathways to achieve catalytic specificity by interfacing redox-active methylene green (MG) and semiconducting graphdiyne (GDY), a 2D multilayered π-staked carbon nanosheet. Optical spectroscopy, electrochemistry, and computational simulation unravel the formation of MG dimers within the interlayer space of GDY nanosheets and the consequential tuning of activation overpotential and electron transfer rates. The electron-hopping pathway by self-exchange of MG dimers in neighboring sheets accelerates oxidation of dihydronicotinamide adenine dinucleotide at 7.06 × 10-2 cm·s-1, while the electron-tunneling pathway directly through GDY film decelerates oxidation of ascorbic acid at 6.60 × 10-5 cm·s-1, further endowing the MG-intercalated GDY nanosheets with high selectivity in mediated bioelectrocatalysis. This study extends the applicability of GDY in selective electrolysis and provides a universal strategy for modulating electrochemical properties of low-dimensional materials with laminar subnano/nanostructure.

11.
Eur J Radiol ; 124: 108816, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31923808

RESUMO

PURPOSE: MR neurography(MRN) is an advanced imaging technique to visualize peripheral nerves. Our aim was to determine the value of morphological features of lumbosacral nerve roots on MRN in diagnosing chronic inflammatory demyelinating polyradiculoneuropathy(CIDP) and analyze their correlations with electrophysiological parameters. METHODS: MRN of lumbosacral plexus was performed in 21 CIDP patients and 21 healthy volunteers. The cross-sectional areas(CSAs) and signal intensities(SI) of L3 to S1 nerve roots were measured and compared between two groups. Receiver operating characteristic(ROC) curves were plotted to assess the diagnostic accuracy. All patients also underwent nerve conduction studies. Correlations between CSAs and SI of lumbosacral nerve roots and electrophysiological parameters were analyzed. RESULTS: Compared with control group, CIDP patients showed significantly increased CSAs and SI from L3 to S1 nerve root (P < 0.001 and P < 0.05 respectively for all nerve roots). The CSAmean and SImean were 28.04 ± 8.55mm2, 1.314 ± 0.199 for patient group and 14.91 ± 2.36mm2,1.155 ± 0.094 for control group. ROC analysis revealed the best diagnostic accuracy for the CSAmean with an area under the curve of 0.968 and optimal cut-off value of 19.20 mm2. CSAs of L5 or S1 nerve root correlated positively with central latency and negatively with conduction velocity of tibial nerve. SI of L5 also had a positive correlation with latency of sural nerve. CONCLUSIONS: Evaluation of lumbosacral nerve roots on MRN in a quantitative manner may serve as an important tool to support the diagnosis of CIDP.

12.
Parasit Vectors ; 13(1): 40, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996262

RESUMO

BACKGROUND: Haemonchus contortus, a blood-feeding parasite, is constantly surrounded by large quantities of heme released from the catabolism of host red blood cells. To cope with the toxicity of free heme, H. contortus needs to uptake and detoxify the heme, a process believed to be paramount for parasite survival. METHODS: A heme-responsive gene Hc-hrg-2 was identified which is the homologue of Ce-hrg-2. The transcriptional levels in all developmental stages and heme-responsive ability of Hc-hrg-2 were analyzed by qRT-PCR. Immunofluorescence analysis and cell transfections were performed to analyze the expression pattern of Hc-HGR-2. Statistical analyses were performed with GraghPad Prism 6.0 using Student's t-test. RESULTS: To investigate the heme homeostasis of H. contortus, we first identified a heme-responsive gene Hc-hrg-2, a homolog of Ce-hrg-2 that is involved in heme transport in the hypodermis of Caenorhabditis elegans. Using qRT-PCR, we showed that Hc-hrg-2 mRNA was expressed throughout all life-cycle stages of H. contortus with the highest level in the third-stage larvae (L3s). Notably, transcription of Hc-hrg-2 in the exsheathed L3s was significantly upregulated in the presence of high concentration of heme. We found that Hc-HRG-2 protein was mainly located in the hypodermal tissues of adult H. contortus in vivo and the endoplasmic reticulum in the transfected mammalian cells. Our in vitro assay demonstrated that Hc-HRG-2 is a heme-binding protein with glutathione S-transferase activity and heme had a significant effect on its enzymatic activity when a model substrate 1-chloro-2, 4-dinitrobenzene (CDNB) was used. CONCLUSIONS: Hc-hrg-2 is a heme-responsive gene and engaged in heme homeostasis regulation in hypodermal tissues during the free-living stages of H. contortus.

13.
Biochem Biophys Res Commun ; 522(4): 862-868, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-31806372

RESUMO

Ebola virus (EBOV), pathogen of Ebola hemorrhagic fever (EHF), is an enveloped filamental RNA virus. Recently, the EHF crisis occurred in the Democratic Republic of the Congo again highlights the urgency for its clinical treatments. However, no Food and Drug Administration (FDA)-approved therapeutics are currently available. Drug repurposing screening is a time- and cost-effective approach for identifying anti-EBOV therapeutics. Here, by combinatorial screening using pseudovirion and minigenome replicon systems we have identified several FDA-approved drugs with significant anti-EBOV activities. These potential candidates include azithromycin, clomiphene, chloroquine, digitoxin, epigallocatechin-gallate, fluvastatin, tetrandrine and tamoxifen. Mechanistic studies revealed that fluvastatin inhibited EBOV pseudovirion entry by blocking the pathway of mevalonate biosynthesis, while the inhibitory effect of azithromycin on EBOV maybe due to its intrinsic cationic amphiphilic structure altering the homeostasis of later endosomal vesicle similar as tamoxifen. Moreover, based on structure and pathway analyses, the anti-EBOV activity has been extended to other family members of statins, such as simvastatin, and multiple other cardiac glycoside drugs, some of which exhibited even stronger activities. More importantly, in searching for drug interaction, we found various synergy between several anti-EBOV drug combinations, showing substantial and powerful synergistic against EBOV infection. In conclusion, our work illustrates a successful and productive approach to identify new mechanisms and targets for treating EBOV infection by combinatorial screening of FDA-approved drugs.

14.
Biochem Biophys Res Commun ; 521(2): 408-413, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31668922

RESUMO

Increasing evidence indicates some G protein-coupled receptors function as a heterodimer, which provide a novel target for therapeutics investigation. However, study on the receptor-receptor interaction interface, a potent target on interfering dimer formation, are still limited. Here, using bioluminescence resonance energy transfer (BRET) combined with co-immunoprecipitation (Co-IP), we found a new constitutive GPCR heterodimer, apelin receptor (APJ)-orexin receptor type 1 (OX1R). Both APJ and OX1R co-internalized when constantly subjected to cognate agonist (apelin-13 or orexin-A) specific to either protomer. Combined with BRET and immunostaining, the in vitro synthesized transmembrane peptides (TMs) interfering experiments suggests that TM4 and 5 of APJ act as the interaction interface of the APJ-OX1R heterodimer, and co-internalization could be disrupted by these peptides as well. Our study not only provide new evidence on GPCR heterodimerization, but address a novel heterodimerization interface, which can be severed as a potential pharmacological target.

15.
IEEE Trans Cybern ; 50(2): 787-797, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30530382

RESUMO

As a fundamental and challenging problem in computer vision, hand pose estimation aims to estimate the hand joint locations from depth images. Typically, the problems are modeled as learning a mapping function from images to hand joint coordinates in a data-driven manner. In this paper, we propose a context-aware deep spatiotemporal network, a novel method to jointly model the spatiotemporal properties for hand pose estimation. Our proposed network is able to learn the representations of the spatial information and the temporal structure from the image sequences. Moreover, by adopting the adaptive fusion method, the model is capable of dynamically weighting different predictions to lay emphasis on sufficient context. Our method is examined on two common benchmarks, the experimental results demonstrate that our proposed approach achieves the best or the second-best performance with the state-of-the-art methods and runs in 60 fps.

16.
IEEE Trans Cybern ; 50(3): 1009-1022, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30418895

RESUMO

Multispectral images contain rich recognition information since the multispectral camera can reveal information that is not visible to the human eye or to the conventional RGB camera. Due to this characteristic of multispectral images, multispectral face recognition has attracted lots of research interest. Although some multispectral face recognition methods have been presented in the last decade, how to fully and effectively explore the intraspectrum discriminant information and the useful interspectrum correlation information in multispectral face images for recognition has not been well studied. To boost the performance of multispectral face recognition, we propose an intraspectrum discrimination and interspectrum correlation analysis deep network (IDICN) approach. Multiple spectra are divided into several spectrum-sets, with each containing a group of spectra within a small spectral range. The IDICN network contains a set of spectrum-set-specific deep convolutional neural networks attempting to extract spectrum-set-specific features, followed by a spectrum pooling layer, whose target is to select a group of spectra with favorable discriminative abilities adaptively. IDICN jointly learns the nonlinear representations of the selected spectra, such that the intraspectrum Fisher loss and the interspectrum discriminant correlation are minimized. Experiments on the well-known Hong Kong Polytechnic University, Carnegie Mellon University, and the University of Western Australia multispectral face datasets demonstrate the superior performance of the proposed approach over several state-of-the-art methods.

17.
Biomed Pharmacother ; 121: 109304, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31810142

RESUMO

Patients with obstructive jaundice are prone to develop cardiovascular complications during surgery. However, the underlying mechanisms remain largely unknown. The present study was aimed to investigate the role of p38 MAPK-pHsp27 pathway in vascular hyporesponsiveness induced by obstructive jaundice. Firstly, an experimental rat obstructive jaundice model was established by bile duct ligation (BDL). We found that the thoracic aorta rings isolated from BDL rats showed decreased response to norepinephrine and acetylcholine, while continuous intraperitoneal injection with SB203580, a selective P38 MAPK inhibitor, could significantly prevented BDL-induced hyporeactivity. Also, the immunohistochemistry and Western blot assays revealed that the up-regulation of pHsp27 and F-actin in thoracic aorta rings from BDL rats and bilirubin-treated vascular smooth muscle cells (VSMCs) were also inhibited by SB203580. Moreover, we identified that bilirubin could induced decreased cell proliferation of VSMCs by using CCK8 assay and which was also prevented by SB203580. All these data demonstrated that p38 MAPK-pHsp27 mediates vascular hyporesponsiveness in rats with obstructive jaundice by modulating the expression level of pHsp27 and F-actin, and that inhibition of p38 MAPK signaling could remodel the vascular activity.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31815008

RESUMO

Right-sided colon cancer (RCC) and left-sided colon cancer (LCC) differ in their clinical and molecular features. An investigation of differentially expressed genes (DEGs) between RCC and LCC could contribute to targeted therapy for colon cancer, especially RCC, which has a poor prognosis. Here, we identified HOXB13, which was significantly less expressed in RCC than in LCC and associated with prognosis in RCC, by using 5 datasets from the Gene Expression Omnibus (GEO). Tissue sample analysis showed that HOXB13 was differentially expressed between normal and only RCC tumor tissues. HOXB13 inhibited colon cancer cell proliferation and induced apoptosis both in vitro and in vivo. Furthermore, we found that HOXB13 might be regulated by DNMT3B and suppress C-myc expression to exert antitumor effects via ß-catenin/TCF4 signals in RCC. In conclusion, the current study is the first to demonstrate that HOXB13 has a tumor-suppressive effect in RCC. High expression levels of HOXB13 are associated with prolonged overall survival in patients with RCC. The DNMT3B-HOXB13-C-myc signaling axis might be a molecular target for the treatment of RCC.

19.
Reprod Domest Anim ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31816136

RESUMO

At present, many three-dimensional (3D) culture systems have been reported, improving the oocyte quality of in vitro maturation (IVM), yet the mechanism still needs to be further explored. Here we examined the effects of a new self-made 3D glass scaffold on buffalo oocyte maturation; meanwhile, the underlying mechanism on buffalo oocyte maturation was also detected. Compared to the two-dimensional (2D) glass dish culture, results revealed that the 3D culture can improve the first polar body rate of oocytes, subsequent cleavage and blastocysts rate of parthenogenetic activation embryos (p < .05). The extracellular matrix-related proteins COL1A1, COL2A1, COL3A1, FN and cell connection-related proteins N-cadherin, E-cadherin, GJA1 were found higher in cumulus cells of 3D culture. Moreover, in cumulus cells, proteins of the PI3K/AKT pathway reported being regulated by FN and E-cadherin including PI3K P85 and p-AKT were also higher in 3D culture. Furthermore, proapoptosis proteins P53, BAX, caspase-3 were lower in both cumulus cells and oocytes in 3D culture, while proteins PCNA and BCL2 showed the opposite result. Results also showed that the apoptosis was inhibited, and the proliferation was enhanced in cumulus cells of 3D culture. Finally, the cumulus expansion-related genes HAS2, CD44, HMMR, PTX3, PTGS2 were found higher in cumulus cells of 3D culture. Taken together, the 3D culture could promote oocyte maturation by regulating proteins correlated with the ECM, cell connection and PI3K/AKT pathway, inhibiting the apoptosis of cumulus cells and oocytes, enhancing the proliferation of cumulus cells and the cumulus expansion.

20.
Animals (Basel) ; 9(12)2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31817264

RESUMO

Pleomorphic adenoma gene 1 (PLAG1) belongs to the PLAG family of zinc finger transcription factors. In cattle, a 19-bp insertion/deletion (indel) was identified in intron 1 of the PLAG1 gene (GenBank Accession No. AC_000171.1). Researches showed that the indel is polymorphic in Chinese cattle breeds such as Qinchuan cattle, Pinan cattle, Xianan cattle, and Jiaxian red cattle, and correlation analysis showed that the polymorphism is related to the height of these cattle breeds. Chinese cattle breeds show a difference in height related to geographical distribution. We investigated the distribution of the 19-bp indel polymorphism in 37 cattle breeds, including 1354 individuals. The results showed that there were three genotypes and two alleles (W, 366 bp; D, 347 bp). From northern cattle to southern cattle, the frequency of W allele gradually decreased, while the frequency of D allele showed an opposite trend, which was consistent with the distribution of cattle breeds of different height in China. Therefore, the polymorphism of this indel may be related to the regional distribution of cattle breeds in China. In addition, we chose Yunling cattle with a mixed genetic background to study the genetic effects of the 19-bp indel on body size traits. Statistical analysis showed that PLAG1 was significantly associated with the body height, cross height, and chest circumference of Yunling cattle (p < 0.05). This study provides new evidence that the 19-bp indel of the PLAG1 gene is a highly effective trait marker that can be used as a candidate molecular marker for cattle breeding.

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