Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 30
Filtrar
1.
Anal Chem ; 92(1): 1463-1469, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31766840

RESUMO

High-sensitivity and high-precision (2 SD ≤ 0.06‰) measurement of chromium (Cr) isotopes at the 10 ng level was successfully carried out using double spike multiple-collector inductively couple plasma mass spectrometry (MC-ICP-MS). To enhance the signal sensitivity and stability, the Aridus II desolvating nebulizer system was improved by placing its waste gas trap bottle in an ice chamber (5 °C cold trap). This setup, beyond Cr isotope analysis, can be applied to most heavy metal isotope measurements. The sensitivity of the 52Cr signal is ≥300 V mg-1 L (with a 1011Ω amplifier and a 110 µL min-1 uptake rate), an enhancement of ≥1.5 times compared to the Aridus II without the cold trap. In addition, the relative standard deviation of the 52Cr signal varied ≤4% over 8 h, demonstrating high stability. The δ53Cr values of common geological reference materials determined using 10 ng of Cr are in excellent agreement with results measured at 25 ng and 50 ng and are consistent with previous determinations, validating the accurate and precise Cr isotope ratio measurements. An empirical method is proposed to correct for the residual (after subtraction) effect of Fe interference on δ53Cr determination. This method relies on a linear relationship between the [Fe]/[Cr] and δ53Cr shift within one analytical session. Finally, we report the δ53Cr values of 19 new reference materials, ranging from -0.44‰ to 0.49‰. Among them, GSS-7 (-0.44 ± 0.02‰, 2 SD, n = 5), GSS-4 (0.48 ± 0.02‰, 2 SD, n = 5), and GSD-10 (0.49 ± 0.05‰, 2 SD, n = 5) can be used as candidate reference materials for interlaboratory comparisons to complement existing ones that are mostly isotopically unfractionated from the bulk silicate earth.

2.
World Neurosurg ; 133: e31-e61, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31415895

RESUMO

BACKGROUND: The association between matrix metalloproteinase 9 (MMP-9) gene -1562C/T (rs3918242) polymorphism and the susceptibility of ischemic stroke (IS) has been investigated. However, results were ambiguous and inconsistent. Therefore, we performed this study to better assess the potential relationship between rs3918242 polymorphism and susceptibility risk of IS. METHODS: We included case-control studies concerning the relationship between the rs3918242 polymorphism and IS, and odds ratios with corresponding 95% confidence intervals were used to describe the associations. Furthermore, meta-regression analyses, heterogeneity, cumulative analyses, sensitivity analyses, and publication bias were examined. RESULTS: A total of 19 studies were included for analysis. Significant associations with the risk of IS were detected for the rs3918242 polymorphism in overall population, Asians, and whites. When available data were stratified by gender, we found a significant correlation with the risk of IS in both males and females. Further subgroup analysis by the subtypes of IS showed that the rs3918242 polymorphism was significantly correlated with the risk of patients with large artery atherosclerosis. When stratified by age, we found that the rs3918242 polymorphism was significantly correlated with the risk of IS in patients both aged ≥65 years and >65 years. Both the diabetes and the nondiabetes subgroups reached significant results, and in an analysis stratified by smoking status, an increased risk of IS was associated with smoking. CONCLUSIONS: The rs3918242 polymorphism may be a susceptible predictor of susceptibility of IS. Further large-scale studies are needed to verify the results of our findings.


Assuntos
Isquemia Encefálica/genética , Metaloproteinase 9 da Matriz/genética , Polimorfismo de Nucleotídeo Único , Adulto , Distribuição por Idade , Idoso , Aterosclerose/epidemiologia , Isquemia Encefálica/enzimologia , Isquemia Encefálica/epidemiologia , Estudos de Casos e Controles , Fumar Cigarros/epidemiologia , Comorbidade , Intervalos de Confiança , Diabetes Mellitus/epidemiologia , Grupos Étnicos , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Embolia Intracraniana/epidemiologia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Distribuição por Sexo
3.
J Stroke Cerebrovasc Dis ; 28(10): 104293, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31383621

RESUMO

BACKGROUND AND AIM: Clot burden score (CBS) was designed to weight the thrombus status in cerebral anterior circulation. We performed a systematic review and meta-analysis to investigate the prognostic value of CBS in acute ischemic stroke (AIS) patients undergoing reperfusion therapies. METHODS: We searched relevant databases for eligible articles reporting CBS in AIS patients. The effect sizes of good functional outcome, recanalization, or hemorrhagic transformation (HT) were pooled with random-/fixed-effect models. Sensitivity analyses and heterogeneity tests were performed. RESULTS: Fifteen eligible studies enrolling 3302 AIS patients undergoing reperfusion therapies were included. AIS patients with per 1-point increase CBS were associated with good functional outcome (pooled odds ratio [OR]: 1.15, 95% confidence interval [CI]: 1.09-1.20) and high rate of recanalization (pooled OR: 1.27, 95% CI: 1.14-1.40). Results from categorical groups indicated high CBS at baseline was associated with higher likelihood of good functional outcome (pooled OR: 1.59, 95% CI: 1.30-1.94) and superior recanalization rates (pooled OR: 2.53, 95% CI: 1.79-3.57). Further stratified analyses showed in intravenous thrombolysis (IVT) alone group, increasing CBS was associated with good functional outcome (continuous pooled OR: 1.18, 95% CI: 1.10-1.27; categorical pooled OR: 3.38, 95% CI: 2.01-5.69) or recanalization (categorical pooled OR: 4.13, 95% CI: 2.00-8.51), but not in endovascular therapy alone group. No significant association was found between CBS and HT. CONCLUSIONS: CBS could be a predictor for AIS after reperfusion therapies in functional outcome and successful recanalization particularly in patients receiving IVT alone; while CBS might not be a predictor for HT.


Assuntos
Isquemia Encefálica/terapia , Procedimentos Endovasculares , Trombose Intracraniana/terapia , Acidente Vascular Cerebral/terapia , Terapia Trombolítica , Idoso , Isquemia Encefálica/diagnóstico por imagem , Isquemia Encefálica/fisiopatologia , Procedimentos Endovasculares/efeitos adversos , Feminino , Humanos , Trombose Intracraniana/diagnóstico por imagem , Trombose Intracraniana/fisiopatologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Fatores de Risco , Acidente Vascular Cerebral/diagnóstico por imagem , Acidente Vascular Cerebral/fisiopatologia , Terapia Trombolítica/efeitos adversos , Resultado do Tratamento
4.
Exp Ther Med ; 18(1): 659-663, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31281446

RESUMO

Curative effect and prognosis of 3D printing titanium alloy trabecular cup and pad in revision of acetabular defect of hip joint were investigated. Forty-two patients who underwent acetabular revision in the Second Affiliated Hospital of Luohe Medical College were divided into observation and control groups according to different methods of acetabular revision and revision materials. 3D printed titanium alloy trabecular cups and pads were used in the observation group, and non-3D printed titanium trabecular cups and pads were used in the control group. Preoperative and postoperative pain visual analog scale (VAS score), hip Harris scores and quality of life Health Survey Scale (SF-36) scores were compared between the groups. At 3, 6 and 12 months after operation, Harris score and SF-36 score of the observation group were significantly higher than those of the control group, and VAS score was significantly lower than that of the control group (P<0.05). Stability and bone ingrowth of prosthesis in the observation group were better than those in the control group. Revision of the hip prosthesis with 3D printed titanium trabecular metal cups and pads resulted in satisfactory outcomes. Short-term prognosis is satisfactory but the long-term prognosis remains to be further investigated.

5.
Exp Ther Med ; 17(5): 3630-3636, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30988746

RESUMO

This study investigated the clinical feasibility and application value of computer virtual reduction combined with three-dimensional (3D) printing technique in patients with complex acetabular fracture. Ninety-six patients diagnosed with complex acetabular fracture in the Orthopedics Department in The Second Affiliated Hospital of Luohe Medical College from January 2016 to June 2017 were selected and randomly divided into the routine operation group (n=48) and the 3D model group (n=48) according to the admission number of the patients. In the 3D model group, computed tomography (CT) scan was performed preoperatively, and the model was made using the virtual technique and 3D printing technique. The surgical scheme was designed according to the model. Patients in the routine operation group were diagnosed with the conventional CT scan without using the computer virtual technique and 3D printing technique. During operation, the operation time, amount of intraoperative bleeding and times of intraoperative fluoroscopy were recorded in both groups. After operation, the incidence rate of such complications as inflammatory response, iatrogenic neurological symptoms and loss of reduction were recorded in both groups. Moreover, the reduction quality of acetabular fracture was evaluated according to the Matta imaging score at 3 days after operation, and the hip joint function was evaluated based on the Hariss score at 6 months after operation. In the 3D model group, the operation time was significantly shorter than that in the control group, the amount of intraoperative bleeding and times of intraoperative fluoroscopy were significantly less than those in the routine operation group, and the incidence rate of postoperative complications was obviously lower than that in the routine operation group (P<0.05). In conclusion, computer virtual reduction combined with the 3D printing technique can significantly reduce the operation time, amount of intraoperative bleeding, times of intraoperative fluoroscopy and incidence rate of postoperative complications without adverse effects on the reduction quality of acetabular fracture and hip joint function of patients, which has a higher clinical application value and greater social significance.

6.
Neurology ; 88(19): 1830-1838, 2017 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-28404799

RESUMO

OBJECTIVE: To examine the efficacy and effect modifiers of folic acid supplementation in the prevention of stroke in regions without folic acid fortification based on relevant, up-to-date published randomized trials. METHODS: Relative risk (RR) was used to measure the effect of folic acid supplementation on risk of stroke using a fixed effects model. FINDINGS: Overall, folic acid supplementation significantly reduced the stroke risk by 11% (22 trials, n = 82,723; RR 0.89, 95% confidence interval [CI] 0.84-0.96). The effect was greater in low folate regions (2 trials, n = 24,020; Asia, 0.78, 0.67-0.90) compared to high folate regions (7 trials, n = 14,655; America, 1.05, 0.90-1.23), and among patients without folic acid fortification (11 trials, n = 49,957; 0.85; 0.77-0.94) compared with those with folic acid fortification (7 trials, n = 14,655; 1.05, 0.90-1.23). In further stratified analyses among trials without folic acid fortification, a larger beneficial effect was found in those trials that used a low dosage of folic acid (≤0.8 mg: 0.78, 0.69-0.88) or low baseline vitamin B12 levels (<384 pg/mL: 0.78, 0.68-0.89). In the corresponding comparison groups, the effect sizes were attenuated and insignificant (p for interaction <0.05 for both). Although the interaction tests were not significant, there might be a higher benefit in trials with a low dosage of vitamin B12, a low prevalence of statin use, but a high prevalence of hypertension. CONCLUSIONS: Folic acid supplementation could reduce the stroke risk in regions without folic acid fortification, particularly in trials using a relatively low dosage of folic acid and with low vitamin B12 levels.


Assuntos
Ácido Fólico/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Acidente Vascular Cerebral/prevenção & controle , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
7.
J Stroke Cerebrovasc Dis ; 25(9): 2126-35, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27363623

RESUMO

BACKGROUND: Many studies have investigated the association between the cyclooxygenase-2 (COX-2) gene polymorphism and ischemic stroke. However, results of these studies still remain controversial. To better explain the association between COX-2 polymorphisms (-765G/C and -1195G/A) and ischemic stroke risk, a meta-analysis was performed. METHODS: Relevant studies were identified from 4 Chinese databases (Chinese Biological Medical Literature database, Chinese National Knowledge Infrastructure database, Chongqing VIP database, and Chinese WANFANG database), PUBMED and EMBASE prior to December 2015. The strength of association between COX-2 polymorphism and ischemic stroke was evaluated by the odds ratio (OR) with 95% confidence interval (CI). Inconsistency index (I(2)) and the Cochran's Q statistic were used to check heterogeneity. Publication bias was evaluated by funnel plots and Egger's regression test. RESULTS: A total of 4086 ischemic stroke cases and 4747 controls were identified. Significant association between COX-2 -765G/C polymorphism and the risk of ischemic stroke was found in Brazilians and the African-Americans. The OR of (CC+GC versus GG) for the Brazilians and African-Americans were (6.328, 95% CI = 2.295-17.448) and (1.644, 95% CI = 1.060-2.551). In addition, the recessive model of the Brazilians gave an OR of 3.621 (95% CI: 1.519-8.630). Furthermore, the (GC versus GG) and the allele model of the African-Americans were (OR: 1.615, 95% CI = 1.015-2.572) and (OR: 1.422, 95% CI = 1.033-1.957). Significant association was also observed for COX-2 -1195G/A polymorphism in the subtypes of small vessel disease (SVD) of ischemic stroke. CONCLUSIONS: Our study suggests that COX-2 -765G/C and -1195G/A polymorphisms may contribute to susceptibility of ischemic stroke, specifically in Brazilians and the African-Americans, and those of SVD.


Assuntos
Isquemia Encefálica/complicações , Isquemia Encefálica/genética , Ciclo-Oxigenase 2/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/genética , Afro-Americanos , Brasil , China , Estudos de Coortes , Estudos Transversais , Análise Mutacional de DNA , Bases de Dados Bibliográficas/estatística & dados numéricos , Feminino , Genótipo , Humanos , Masculino , Metanálise como Assunto
8.
Int J Neurosci ; 126(3): 219-26, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26001207

RESUMO

BACKGROUND: The common and major pathological change in ischemic stroke is atherosclerosis in the artery. Tumor necrosis factor-a (TNF-a) is closely related to the pathogenesis of atherosclerosis. The aim of our study was to investigate whether TNF-a gene variants (-238G/A and -308G/A) are associated with ischemic stroke. METHODS: A total of 619 ischemic stroke patients and 612 controls were recruited to estimate the frequencies of two TNF-a (-238G/A and -308G/A) single nucleotide polymorphisms using a Sequenom MassARRAY time-of-flight mass spectrometer. The association between TNF-a gene polymorphisms and ischemic stroke risk was evaluated by computing the odds ratio (OR) and 95% Confidence Interval with multivariate unconditional logistic regression analyses. RESULTS: The OR results indicated that no significant associations were found between TNF-a gene (-238G/A and -308G/A) polymorphisms and the risk of ischemic stroke using five genetic models, including the allele model (A vs. G), co-dominant model 1 (GA vs. GG), co-dominant model 2 (AA vs. GG), the dominant model (AA+GA vs. GG), and the recessive model (GG+GA vs. AA). CONCLUSIONS: The TNF-a (-238G/A and -308G/A) gene polymorphisms may not be a susceptible predictor of ischemic stroke in Chinese populations.


Assuntos
Isquemia Encefálica/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Acidente Vascular Cerebral/genética , Fator de Necrose Tumoral alfa/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Genéticos , Fatores de Risco
9.
Rejuvenation Res ; 19(4): 303-8, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26649780

RESUMO

With the average life span of humans on the rise, aging in the world has drawn considerable attentions. The monoamine neurotransmitters and neurotrophic factors in brain areas are involved in learning and memory processes and are an essential part of normal synaptic neurotransmission and plasticity. In the present study, the effect of Zhuang Jing Decoction (ZJD) on the learning and memory ability in aging rats was examined in vivo using Morris water maze. Furthermore, the levels of monoamine neurotransmitters and neurotrophic factors in brain were detected by high-performance liquid chromatography with a fluorescence detector and enzyme-linked immunosorbent assay, respectively. These data showed that oral administration with ZJD at the dose of 30 g·kg(-1) exerted an improved effect on learning and memory ability in aging rats. The results revealed that ZJD could effectively adjust the monoamine neurotransmitters and neurotrophic factors, restore the balance of the level of monoamine neurotransmitters and neurotrophic factors in brain, and finally attenuate the degeneration of learning and memory ability. These findings suggested that ZJD might be a potential agent as cognitive-enhancing drug in improving learning and memory ability. It may exert through regulating the levels of monoamine neurotransmitters and neurotrophic factors in brain, which demonstrated that ZJD had certain antiaging effects.


Assuntos
Envelhecimento/psicologia , Comportamento Animal/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Fatores Etários , Envelhecimento/metabolismo , Animais , Encéfalo/metabolismo , Cromatografia Líquida de Alta Pressão , Dopamina/metabolismo , Relação Dose-Resposta a Droga , Ensaio de Imunoadsorção Enzimática , Feminino , Fatores de Crescimento Neural/metabolismo , Norepinefrina/metabolismo , Ratos Sprague-Dawley , Serotonina/metabolismo , Espectrometria de Fluorescência , Fatores de Tempo
10.
J Stroke Cerebrovasc Dis ; 24(7): 1577-90, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25890854

RESUMO

BACKGROUND: Several investigations have been performed to examine the influence of the ß-fibrinogen (FGß) gene polymorphisms on the risk of ischemic stroke, but the results of these studies are controversial. Our study aimed at investigating whether the FGß gene (-148 C/T, 448 G/A, and -854 G/A) polymorphisms were associated with susceptibility to ischemic stroke by conducting meta-analysis. METHODS: Relevant studies were identified from 4 Chinese databases, PUBMED and EMBASE before May 30, 2014. The strength of association was evaluated by the odds ratio with 95% confidence interval. Inconsistency index and the Cochran's Q statistic were used to check heterogeneity. Publication bias was tested using funnel plots and Egger's regression test. RESULTS: Thirty-two independent studies with 4311 cases and 4124 controls were included. Significant association between -148 C/T polymorphism and the risk of ischemic stroke was found in overall analysis and middle-age, but not in young adults and elderly people. Similarly, association was also observed for -854 G/A polymorphism, especially in cerebral arterial main trunk infarction (MCI) and cerebral penetrating arterial infarction (PCI). However, no significance was found between 448 G/A polymorphism and ischemic stroke in Chinese people; likewise, no evidence of a significant association was observed when stratified according to the subtype of ischemic stroke (MCI and PCI). CONCLUSIONS: These results suggest that -148 C/T and -854 G/A polymorphisms probably contribute to susceptibility of ischemic stroke.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Isquemia Encefálica/genética , Fibrinogênio/genética , Polimorfismo Genético , Acidente Vascular Cerebral/genética , Isquemia Encefálica/diagnóstico , Isquemia Encefálica/etnologia , Estudos de Casos e Controles , China/epidemiologia , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Razão de Chances , Fenótipo , Fatores de Risco , Acidente Vascular Cerebral/diagnóstico , Acidente Vascular Cerebral/etnologia
11.
Epilepsy Res ; 108(6): 1052-67, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24794827

RESUMO

Previous studies have attempted to confirm the association between the ABCB1-C3435T polymorphism and drug-resistant epilepsy and produced discordant findings. A meta-analysis was conducted to assess the role of the C3435T polymorphism in drug-resistance in epilepsy. Databases were obtained from PubMed, Embase, the Chinese Wanfang, CNKI, and Chongqing VIP database, and all relevant studies were compiled up to February 2013. Odds ratios (ORs) were calculated using models of both fixed- and random-effects for comparisons of alleles and genotypes. Subgroup meta-analyses were carried out based on epilepsy subtype, age, therapeutic regimen, definition of drug-responsiveness and drug-resistance using alleles and genotypes models. Publication bias was tested by Begg's test and inverted funnel plot, and heterogeneity was checked by Cochran's Q statistic and the inconsistency index (I2). Cumulative meta-analyses were adopted to test the robustness of the findings. A total of 38 association studies including a total of 8716 subjects, 4037 drug-resistant patients and 4679 drug-responsive epilepsy patients were pooled in this meta-analysis. The association of ABCB1-C3435T with risk of drug-resistance was significant in the overall population (T allele vs. C allele, OR: 1.21; 95%CI: 1.06-1.39; P=0.006) and in Caucasians, adults, groups treated with various drugs, a '>10 seizures in a year' group based on resistance and a '≥2 years seizure free' group based on response subgroup analysis. The ABCB1-C3435T polymorphism is likely to act as a risk factor for resistance to antiepileptic drugs that needs to be confirmed through further studies.


Assuntos
Anticonvulsivantes/uso terapêutico , Resistência a Medicamentos/genética , Epilepsia/tratamento farmacológico , Epilepsia/genética , Polimorfismo de Nucleotídeo Único , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Adolescente , Adulto , Fatores Etários , Grupo com Ancestrais do Continente Asiático/genética , Biomarcadores Farmacológicos , Criança , Bases de Dados Genéticas , Grupo com Ancestrais do Continente Europeu/genética , Genótipo , Humanos , Pessoa de Meia-Idade , Fatores de Risco , Adulto Jovem
12.
Thromb Res ; 133(6): 993-1005, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24448059

RESUMO

BACKGROUND: Ischemic stroke (IS) and coronary heart disease (CHD) are two vascular disorders that are a common cause of death worldwide. Several studies have assessed the association of the ß-fibrinogen-455G/A (FGB-455G/A) polymorphism and risk of IS and CHD, but the results are still inconsistent. Our study aimed to investigate whether the FGB-455G/A polymorphism was associated with susceptibility to IS and CHD by using meta-analysis. METHODS: Relevant studies were identified from PubMed, Embase and four Chinese database up to July 2013.Data were analyzed and processed by Stata 11.2. A pooled OR with 95% CI was calculated to estimate the strength of the genetic association. Cumulative meta-analysis was performed to assess the tendency of pooled OR over time. RESULTS: 45 studies based on a total of 7238 cases and 7395 controls were included in our meta-analysis. The results indicated that the FGB-455G/A polymorphism is associated with the risk of IS when compared with the dominant model (OR=1.518, 95%CI=1.279-1.802 for AA+GA vs. GG). In the subgroup analysis by ethnicity, significantly elevated risks were associated with the A allele in Asians (OR=1.700, 95%CI=1.417-2.040), but not in Caucasians (OR=0.942, 95%CI=0.813-1.091). Both the hypertension and non-hypertension subgroups reached significant results, but no significance was found when stratified according to sex or subtype of IS. Results indicate that the FGB-455G/A polymorphism is associated with CHD (OR=1.802, 95%CI=1.445-2.246). CONCLUSION: Our meta-analysis suggests that the FGB-455G/A polymorphism contributes to susceptibility to IS and CHD.


Assuntos
Doença da Artéria Coronariana/genética , Fibrinogênio/genética , Infarto do Miocárdio/genética , Acidente Vascular Cerebral/genética , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Polimorfismo de Nucleotídeo Único , Medição de Risco
13.
Int J Neurosci ; 124(11): 841-51, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24397542

RESUMO

BACKGROUND: The role of genetic variants in the pathogenesis of stroke has not been fully elucidated. Several studies have been examined the association of the Integrin alpha2 (ITGA2) gene-C807T (rs1126643) polymorphism with ischemic stroke susceptibility. However, the results of these studies are inconsistent. In order to explore this association more deeply, we performed a meta-analysis. METHODS: We collected case-control studies concerning the relationship between the C807T polymorphism and ischemic stroke, and odd ratios (OR) with corresponding 95% confidence intervals (CI) were used to describe the relationships. Inconsistency index (I(2)) and Cochran's Q statistic were used to check heterogeneity. Publication bias was tested by funnel plots and Egger's test. RESULTS: Fifteen studies with 2242 cases and 2408 controls were included. Our meta-analysis results indicated an association between the C807T polymorphism and the risk of ischemic stroke in the overall population, Asians and the subgroup of hospital-based people. However, statistically association was not observed for Caucasians and non-hospitalized individuals. CONCLUSIONS: The ITGA2 gene C807T polymorphism may be a susceptible predictor of the risk of ischemic stroke. More data are needed to elucidate the relationship further.


Assuntos
Isquemia Encefálica/genética , Predisposição Genética para Doença/genética , Integrina alfa2/genética , Polimorfismo de Nucleotídeo Único/genética , Acidente Vascular Cerebral/genética , Grupo com Ancestrais do Continente Asiático/genética , Isquemia Encefálica/complicações , Estudos de Casos e Controles , Grupo com Ancestrais do Continente Europeu/genética , Estudos de Associação Genética , Hospitalização/estatística & dados numéricos , Humanos , Viés de Publicação/estatística & dados numéricos , Acidente Vascular Cerebral/complicações
14.
Clin Biochem ; 47(6): 409-16, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24345348

RESUMO

BACKGROUND: Recently, studies have focused on the association between the p22phox gene A640G polymorphism and coronary heart disease (CHD). However, the results are inconsistent. In this study, we aimed to further evaluate this association by using meta-analysis. METHODS: The PubMed, Embase, CBM, CNKI, WanFang and Chongqing VIP databases were searched for relevant articles. Hardy-Weinberg equilibrium (HWE) of the distribution of genotypes was tested using Pearson's chi-squared test. Odds ratios (ORs) with the corresponding 95% confidence intervals (CIs) were used to assess the strength of the association; Cochran's Q test and the I(2) statistic were used to evaluate heterogeneity. The random effects model and the fixed effects model were used according to heterogeneity; Begg's test and Egger's test were used to analyze publication bias. Sensitivity analysis was carried out to guarantee the stability of the results. Cumulative analysis was used to evaluate tendencies in the pooled OR. RESULTS: A total of eight articles including 3904 CHD cases and 3498 controls were included. A significant association between the A640G polymorphism and CHD was observed in codominant model 2 (AG versus AA: OR=0.86, 95% CI: 0.77-0.96). In the subgroup analysis, a significant association was observed between the A640G polymorphism and CHD in Caucasians, and in PB (population-based), non-PB, HWE (studies followed HWE) and non-HWE studies. CONCLUSIONS: Our results reveal that the A640G polymorphism may play a protective role in CHD.


Assuntos
Doença das Coronárias/enzimologia , Doença das Coronárias/genética , Predisposição Genética para Doença , NADPH Oxidases/genética , Polimorfismo de Nucleotídeo Único/genética , Comportamento de Redução do Risco , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Viés de Publicação
15.
Neurol Sci ; 35(2): 151-61, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24366241

RESUMO

Many studies have investigated the association between the ß-fibrinogen gene-455G/A (FGß-455G/A) polymorphism and the risk of ischemic stroke. However, these evidences were inadequate to provide stronger conclusions because most studies were generally small. To shed light on these inconclusive findings, we conducted a large sample size meta-analysis of studies relating to the FGß-455G/A polymorphism and the risk of ischemic stroke. Odds ratios with a 95 % confidence interval were used to investigate the association between FGß-455G/A polymorphism and ischemic stroke. Publication bias was tested by Egger's test and funnel plot. Inconsistency index and Cochran's Q statistic were used to check heterogeneity. Cumulative and recursive cumulative meta-analyses were performed to provide a framework for updating a genetic effect from all of the included studies. Twenty-six independent publications with 4,070 cases and 4,649 controls were included in this meta-analysis. Results showed that the ß-fibrinogen-455G/A polymorphism was significantly associated with the risk of ischemic stroke. The FGß-455G/A polymorphism was found to be a risk factor for ischemic stroke in Asians and adults, while association was not observed for Caucasians and juveniles based on the small size and it may be necessary to conduct larger studies on them to investigate the association in the future. The cumulative meta-analysis indicated a decline from 1998 to 2003, and the results remained stable during the period 2004-2012. The results indicate that FGß-455G/A polymorphism may be a susceptible predictor of ischemic stroke. More studies are needed to elucidate the relationship further.


Assuntos
Isquemia Encefálica/genética , Fibrinogênio/genética , Predisposição Genética para Doença , Polimorfismo Genético , Acidente Vascular Cerebral/genética , Fatores Etários , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Isquemia Encefálica/epidemiologia , Grupo com Ancestrais do Continente Europeu/genética , Humanos , Modelos Genéticos , Razão de Chances , Viés de Publicação , Fatores de Risco , Acidente Vascular Cerebral/epidemiologia
16.
J Neurol Sci ; 338(1-2): 3-11, 2014 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-24365247

RESUMO

Iceland scientists identified the relationship between the PDE4D gene and ischemic stroke (IS) in 2003. Since then, many researches have emerged to estimate this association, but the results are contradictory. In order to confirm this association, we conduct this meta-analysis with a larger sample size. PubMed, Embase and four Chinese databases were searched to identify the relevant studies through January 2013. The odds ratio (OR) with 95% confidence interval (CI) was used to calculate the association between the SNP83 polymorphism and IS risk. Twenty-five publications comprised of 8878 cases and 12306 controls were included in this meta-analysis. There was a significant association between SNP83 and IS risk, especially in Asian and Chinese populations, but not in Caucasians (dominant model: OR=0.87, 95% CI=0.69-1.11; recessive model: OR=0.95, 95% CI=0.84-1.07; allele model: OR=0.95, 95% CI=0.84-1.08; co-dominant model 1: OR=0.96, 95% CI=0.85-1.08; co-dominant model 2: OR=0.95, 95% CI=0.83-1.09). The cumulative meta-analysis among the overall population and Chinese population indicated a stable trend of association between SNP83 and IS from 2009 to 2012. In conclusion, we found an association between SNP83 and IS in the overall population and in the Asian and Chinese populations, but not among Caucasians.


Assuntos
Isquemia Encefálica/genética , Nucleotídeo Cíclico Fosfodiesterase do Tipo 4/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Acidente Vascular Cerebral/genética , Isquemia Encefálica/complicações , Bases de Dados Factuais/estatística & dados numéricos , Estudos de Associação Genética , Humanos , Acidente Vascular Cerebral/etiologia
17.
Int J Endocrinol ; 2013: 753150, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24288530

RESUMO

Diabetes mellitus (DM) is one of the primary causes of premature death and disability worldwide. We performed a systematic review and meta-analysis of the published literature regarding the trends in prevalence, awareness, treatment, and control of diabetes mellitus in mainland China. PUBMED, EMBASE, Chinese Biomedical Database, China National Infrastructure database, Chinese Wan Fang database, and Chongqing VIP database were searched. Fifty-six eligible studies were included. Increasing trends in the prevalence, treatment, and control of diabetes in mainland China from 1979 to 2012 were observed. The pooled prevalence, awareness, treatment, and control of diabetes mellitus were 6.41%, 45.81%, 42.54%, and 20.87%, respectively. A higher prevalence of diabetes mellitus was found in urban (7.48%, 95%CI = 5.45~9.50) than rural (6.53%, 95%CI = 4.30~8.76) areas. Furthermore, an increasing chronological tendency was shown in different subgroups of age with regard to the prevalence of diabetes. A higher awareness of DM was found in urban (44.25%, 95%CI = 32.60~55.90) than rural (34.27%, 95%CI = 21.00~47.54) populations, and no significant differences were found in the treatment, and control of diabetes among the subgroups stratified by gender and location. From 1979 to 2012, the prevalence, treatment, and control of diabetes mellitus increased; nevertheless, there was no obvious improvement in the awareness of diabetes.

18.
J Neurol Sci ; 331(1-2): 118-25, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23790236

RESUMO

Recently, the association between AGT M235T polymorphism and ischemic stroke (IS) has attracted widespread attention, and many investigations have been performed. However, the results were inconsistent. Therefore, we performed a meta-analysis to further evaluate the association between M235T and IS. All of the relevant studies were identified from PubMed, EMBASE, Chinese National Knowledge Infrastructure database (CNKI), Chinese Biological Medical Literature database (CBM), Chinese Wanfang and Chongqing VIP database up to January 2013. Statistical analyses were conducted with STATA software version 11.1. Odds ratios with 95% confidence interval were applied to evaluate the strength of the association. We performed the cumulative meta-analysis to assess the tendency of pooled OR over time. Heterogeneity was evaluated by Q-test and the I(2) statistic. The funnel plots and Egger's regression test were used to assess the publication bias. A significant association between AGT M235T polymorphism and IS was found under the dominant model (OR=1.368, 95% CI=1.070-1.749), recessive model (OR=1.66, 95% CI=1.310-2.103), over-dominant model (OR=1.285, 95% CI=1.085-1.523), co-dominant model (OR=1.574, 95% CI=1.276-1.942) and allele model (OR=1.447, 95% CI=1.207-1.735). Besides the Caucasian and the population-based controls, significant association could be found in the subgroup analysis of Asian and hospital-based controls. Results from cumulative analysis showed a tendency of significant association of this polymorphism with IS. However, the opposite trend was observed among Caucasians. Results from our meta-analysis indicated that the AGT M235T polymorphism might be a risk factor for IS among Asians, but not for Caucasians. More studies are required to further confirm our findings.


Assuntos
Angiotensinogênio/genética , Predisposição Genética para Doença , Metionina/genética , Acidente Vascular Cerebral/genética , Treonina/genética , Grupo com Ancestrais do Continente Asiático , Bases de Dados Factuais , Grupo com Ancestrais do Continente Europeu , Estudos de Associação Genética , Humanos , Isquemia/complicações , Acidente Vascular Cerebral/etiologia
19.
PLoS One ; 8(6): e65356, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23785419

RESUMO

BACKGROUND: Major depressive disorder (MDD) is one of the important causes of disease burden in the general population. Given the experiencing rapid economic and social changes since the early 1990s and the internationally recognized diagnostic criteria and interview instruments across the surveys during 2001-2010 in china, the epidemiological studies on MDD got varied results. We performed this meta-analysis to investigate current, 12-month and lifetime prevalence rates of MDD in mainland China. METHODS: PubMed, Embase, Chinese Biological Medical Literature database (CBM), Chinese National Knowledge Infrastructure database (CNKI), and the Chinese Wanfang and Chongqing VIP database were searched for associated studies. We estimated the overall prevalence of MDD using meta-analysis. CONCLUSIONS: Seventeen eligible studies were included. Our study showed that the overall estimation of current, 12-month and lifetime prevalence of MDD was 1.6, 2.3, 3.3%, respectively. The current prevalence was 2.0 and 1.7% in rural and urban areas, respectively; between female and male, it was 2.1 and 1.3%, respectively. In addition, the current prevalence of MDD diagnosed with SCID (Structured Clinical Interview for DSM-IV) was 1.8% and that diagnosed with CIDI (Composite International Diagnostic Interview) was 1.1%. In conclusion, our study revealed a relatively high prevalence rate in the lifetime prevalence of MDD. For current prevalence, MDD diagnosed with SCID had a higher prevalence rate than with CIDI; males showed a lower rate than females, rural residents seemed to have a greater risk of MDD than urban residents.


Assuntos
Transtorno Depressivo Maior/epidemiologia , China/epidemiologia , Transtorno Depressivo Maior/prevenção & controle , Feminino , Humanos , Masculino , Prevalência , Fatores Sexuais
20.
J Neurol Sci ; 330(1-2): 100-10, 2013 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-23684671

RESUMO

Recently, increasing studies have been focused on the association between the p22phox gene C242T polymorphism and ischemic stroke (IS). However, the results were controversial. As far as we know, there is no previous systematic review or meta-analysis concerning this association. Thus, we conducted this meta-analysis to evaluate this association. The strength of association was evaluated by the odds ratio (OR) with the corresponding 95% confidence intervals (CIs). Heterogeneity was assessed by Q-test and the I(2) statistic. Publication bias was tested using funnel plots and Egger's regression test. Cumulative meta-analysis was performed to assess the trend in pooled OR over time. There was no significant association of the p22phox gene C242T polymorphism with IS in the overall analysis and subgroup analysis by ethnicity and subtypes of IS. However, statistical significance was found in the dominant model (OR: 1.57, 95%CI: 1.18-2.09), codominant model (OR: 1.62, 95%CI: 1.20-2.17) and allelic model (OR: 1.44, 95%CI: 1.11-1.87) among the hospital-based studies. The cumulative meta-analysis also suggested no trend of association between this polymorphism and IS from 2007 to 2011 as more data accumulated over time. Our meta-analysis indicated that the p22phox gene C242T polymorphism is unrelated to the risk of IS in the overall analysis and subgroup analysis by ethnicity and subtypes of IS. However, statistical significance was found in the subgroup analysis by source of controls among the hospital-based studies.


Assuntos
Isquemia Encefálica/genética , NADPH Oxidases/genética , Polimorfismo Genético/genética , Acidente Vascular Cerebral/genética , Alelos , Grupo com Ancestrais do Continente Asiático , Interpretação Estatística de Dados , Grupo com Ancestrais do Continente Europeu , Genes Dominantes , Estudos de Associação Genética , Predisposição Genética para Doença , Genótipo , Humanos , Modelos Genéticos , Viés de Publicação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA