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1.
Biomed Res Int ; 2018: 1415082, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30426002

RESUMO

Digeda-4 decoction is a traditional Mongolian medicine; its effects on cytochrome (CYP) enzymes are still unclear. CYP450 isoenzymes are the main drug metabolic enzymes, and their activities may be induced or inhibited by certain drugs, which lead to drug interactions in clinical use. Effects of Digeda-4 decoction on the activities of CYP450 subtype enzymes CYP1A2, CYP2C9, CYP2E1, CYP2C19, and CYP3A4 in rats were studied by cocktail method, and the pharmacokinetic parameters of five specific probe drugs (theophylline, tolbutamide, chlorzoxazone, omeprazole, and midazolam) were calculated by DAS software; changes of parameters can be used to evaluate the effects of Digeda-4 decoction on enzyme activities. The experimental rats were divided into three groups: control group, Digeda group, and positive group. Rats in Digeda group were given Digeda-4 decoction through continuous gavage for 14 days. After fasting for 12 hours, the mixed probes drug solution was injected into the tail vein; the blood samples were collected through the orbital vein at different time points. The concentrations of probe drugs in rat plasma were measured by HPLC. Compared with the control group, the half-life time (t1/2) of the pharmacokinetic parameters of theophylline, tolbutamide, omeprazole, and midazolam was prolonged, the area under the curve (AUC) increased, and the plasma clearance (CL) decreased in the Digeda group. Continuous gavage administration for 14 days may inhibit the activities of CYP450 subtype enzymes CYP1A2, CYP2C9, CYP2C19, and CYP3A4 of rats. Herb-drug interaction should be noted between Digeda-4 decoction and the drugs metabolized by CYP1A2, CYP2C9, CYP2C19, and CYP3A4.


Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Animais , Cromatografia Líquida de Alta Pressão/métodos , Interações Ervas-Drogas , Masculino , Medicina Tradicional do Leste Asiático/métodos , Ratos , Ratos Wistar
2.
Huan Jing Ke Xue ; 39(4): 1688-1696, 2018 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964994

RESUMO

High salt content could result in the inhibition of microbes and affect biological treatment processes. At present, an important research topic is how to improve the efficiency of biological treatments. The anaerobic ammonia oxidation (ANAMMOX) process was used to treat high saline wastewater. Nitrogen removal performance with betaine was studied by analyzing the ANAMMOX activity, and ammonia nitrogen and nitrate nitrogen removal. The results showed that:① It has obvious improvement when betaine concentration was 0.1-0.4 mmol·L-1. It alleviated the salt stress on bacteria growth inhibition of ANAMMOX, and also promoted the growth of denitrifying bacteria. When betaine concentration was 0.4-0.5 mmol·L-1, denitrifying bacteria was found to have grown greatly. When betaine concentration was greater than 0.5 mmol·L-1, it was unable to alleviate the salt stress inhibiting denitrification efficiency. As a result, betaine concentration of 0.8 mmol·L-1 completely inhibited bacteria. ② When concentration of betaine was 0.3 mmol·L-1, the optimal nitrogen removal efficiency was achieved. NH4+-N and NO2--N increased by 16% and 32%, respectively. Nitrogen removal rate (NRR) increased by 26.8%. ③ At the end of the recovery experiment, with the decreasing concentrations of betaine, NH4+-N was 50.6%, NO2--N was 63.7%, and NRR was 0.65 kg·(m3·d)-1, so the nitrogen removal efficiency underwent fast recovery.


Assuntos
Betaína/química , Reatores Biológicos/microbiologia , Desnitrificação , Nitrogênio/isolamento & purificação , Águas Residuárias/química , Purificação da Água/métodos , Bactérias/crescimento & desenvolvimento , Oxirredução , Salinidade
3.
Angew Chem Int Ed Engl ; 56(52): 16510-16514, 2017 12 22.
Artigo em Inglês | MEDLINE | ID: mdl-29071780

RESUMO

The utility of electronically conductive metal-organic frameworks (EC-MOFs) in high-performance devices has been limited to date by a lack of high-quality thin film. The controllable thin-film fabrication of an EC-MOF, Cu3 (HHTP)2 , (HHTP=2,3,6,7,10,11-hexahydroxytriphenylene), by a spray layer-by-layer liquid-phase epitaxial method is reported. The Cu3 (HHTP)2 thin film can not only be precisely prepared with thickness increment of about 2 nm per growing cycle, but also shows a smooth surface, good crystallinity, and high orientation. The chemiresistor gas sensor based on this high-quality thin film is one of the best room-temperature sensors for NH3 among all reported sensors based on various materials.

4.
Huan Jing Ke Xue ; 38(12): 5162-5168, 2017 Dec 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964577

RESUMO

An anaerobic sequencing batch reactor (ASBR) was used to study substrate inhibition and kinetic characteristics of marine anaerobic ammonium oxidizing bacteria (MAAOB) treating saline wastewater. The results indicated that when ammonia increased to 1200 mg·L-1, the MAAOB still maintained good nitrogen removal capability, though there was a slight inhibitory effect. At the same time, nitrite nitrogen removal efficiency was stable at about 80.70%. When nitrite increased to 265.6 mg·L-1, the MAAOB were inhibited obviously, and ammonia nitrogen removal efficiency decreased to about 63.01%. When influent nitrite concentration increased to 305.6 mg·L-1, the removal rate of ammonia nitrogen further decreased to 43.93%. The kinetic characteristics resulting from inhibition of the MAAOB were simulated by the Haldane model and Aiba model. Three parameters, TNRRmax, KS, and Ki, and the relationship between effluent substrate concentration and total nitrogen loading (TNRR) were evaluated. Based on further analysis, the Haldane model was more suitable for describing dynamic characteristics resulting from NH4+-N inhibition, while the Aiba model was more suitable for describing the dynamic characteristics resulting from NO2--N inhibition. The predicted effluent inhibitory concentrations of NH4+-N and NO2--N were 3893.625 mg·L-1 and 287.208 mg·L-1, respectively. The results could provide a theoretical basis for saline wastewater treatment by MAAOB.


Assuntos
Compostos de Amônio/metabolismo , Bactérias Anaeróbias/metabolismo , Nitrogênio/isolamento & purificação , Águas Residuárias , Reatores Biológicos , Oxirredução , Salinidade
5.
Huan Jing Ke Xue ; 38(7): 2917-2924, 2017 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964633

RESUMO

In view of the poor performance of biological nitrogen removal in saline wastewater treatment, nitrogen removal of anaerobic ammonium oxidation(ANAMMOX) with K+ addition was studied. The results showed that K+ addition could strengthen nitrogen removal performance. The effect of K+ on ANAMMOX process was mainly divided into four stages. In the adaptive phase(0-2 mmol·L-1), sudden addition of K+ destroyed the original equilibrium reaction, but ultimately ANAMMOX bacteria could adapt to the presence of K+. Because K+ had no obvious effects on anaerobic ammonia oxidation bacteria, the removal rates of NH4+-N and NO2--N slightly increased. In the ascension phase(2-8 mmol·L-1), K+ played a role in promoting anaerobic ammonia oxidation biological systems, with increasing concentration of K+, the removal rates of NH4+-N and NO2--N significantly increased. In the stabilization phase(8-20 mmol·L-1), the nitrogen removal rate decreased, but was still higher than the control without K+ addition. In the inhibition phase(more than 20 mmol·L-1), K+ largely inhibited anaerobic ammonia oxidation, and its denitrification efficiency was lower than 0 mmol·L-1. In the whole cycle, when the concentration of K+ was 8 mmol·L-1, it achieved the best effect, the average removal rates of NH4+-N and NO2--N were 89.24% and 84.87%, and NRR was 1.113 kg·(m3·d)-1.


Assuntos
Reatores Biológicos , Desnitrificação , Nitrogênio/isolamento & purificação , Potássio/química , Bactérias Anaeróbias , Oxirredução , Salinidade , Águas Residuárias
6.
Huan Jing Ke Xue ; 38(8): 3369-3376, 2017 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964946

RESUMO

The effect of pH shock on nitrogen removal performance of marine anaerobic ammonium-oxidizing bacteria (MAAOB) treating saline wastewater was studied by employing an ASBR reactor. Dynamic characteristics of the MAAOB were simulated by the Andrew model and Ratkowsky model. The results indicated that the reactor had the best nitrogen removal efficiency when the pH value was 7-8. The nitrogen removal rate (NRR) was (0.30±0.04) kg·(m3·d)-1, and the total nitrogen removal efficiency (TNRE) was (76.73±5.74)%. When the pH value was 8.5, FA had a mean concentration of 14.22 mg·L-1 and little effect on nitrogen removal. The NRR was (0.30±0.02) kg·(m3·d)-1. However, NO2--N accumulated and it was not completely removed. When the pH values were 6.5 and 9, the concentrations of FA were 0.22 mg·L-1 and 37.84 mg·L-1, respectively, the NRRs were (0.10±0.02) and (0.15±0.02) kg·(m3·d)-1, and the TNREs were (23.04±9.88)% and (42.12±5.52)%. The tolerance of the MAAOB in alkaline condition was stronger than that in acidic condition. The Andrew model was modified to determine the relationship between NRR and FA. Other parameters such as NRRmax, kS, and kI were also achieved simultaneously. These are key to describing the nitrogen removal process of MAAOB.


Assuntos
Compostos de Amônio/isolamento & purificação , Bactérias Anaeróbias/metabolismo , Reatores Biológicos/microbiologia , Águas Residuárias , Purificação da Água , Desnitrificação , Nitrogênio/isolamento & purificação , Oxirredução , Salinidade
7.
Huan Jing Ke Xue ; 38(5): 2044-2051, 2017 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965112

RESUMO

The effect of temperature on the nitrogen removal performance of marine anaerobic ammonium oxidizing bacteria processing sewage seawater was studied by employing an ASBR reactor, and the dynamic characteristics of the marine anaerobic ammonium oxidizing bacteria at different temperatures were simulated by modified Logistic model. The experimental results indicated that the nitrogen removal performance was affected little at 25-35℃. The total nitrogen removal efficiency (TNRE) remained at (82±2)% and the total nitrogen removal rate (TNRR) was stabilized at (0.62±0.01) kg·(m3·d)-1. When the temperature was 20℃, TNRE increased from 59% at the beginning to 79% after 13 days. This indicated that the marine anammox bacteria still had strong ability of nitrogen removal, and the reactor in the low temperature treatment of sewage containing seawater had a good potential. However, when the temperature dropped to 10-15℃, the nitrogen removal performance of the reactor was inhibited. TNRE decreased to (40±8)% and (11±4)%, respectively. Besides, TNRR also decreased to (0.30±0.04) kg·(m3·d)-1 and (0.08±0.03) kg·(m3·d)-1, respectively. According to the Arrhenius equation, the activation energy for marine anaerobic ammonium oxidation reaction was 26 kJ·mol-1 at 25-35℃, and the activation energy of marine anaerobic ammonium oxidation reaction was 76 kJ·mol-1 at 10-25℃. In addition, dynamic analysis was performed by Logistic model and the NRE and effluent total nitrogen concentration (ceff) at different temperatures were forecasted. The correlation coefficient R2 was between 0.9668 and 0.9957.


Assuntos
Compostos de Amônio/metabolismo , Bactérias Anaeróbias/metabolismo , Desnitrificação , Nitrogênio/isolamento & purificação , Temperatura , Anaerobiose , Reatores Biológicos , Oxirredução , Água do Mar , Esgotos
8.
Zhongguo Zhong Yao Za Zhi ; 40(5): 771-7, 2015 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-26087532

RESUMO

Mongolian folk medicine resource is the origin of Mongolian medicine development, even more important of which is the specialized Mongolian folk medicine resources with regional and high medicine quality, it processes distinctive national characteristics with irreplaceable important position in traditional Mongolian medicine. Nevertheless, due to the serious destroy of ecological environment and sharp increase of demands, etc. A lot of specialized Mongolian folk medicine resources were endangered, and there still existed some problems in the protection and exploitation and utilization. This paper intends to provide comprehensive insight into the species protection and exploitation and utilization states of specialized Mongolian folk medicine resources. The application and protection status and the existing problems were reviewed, and the development strategies of Mongolian folk medicine resource were analyzed.


Assuntos
Conservação dos Recursos Naturais , Medicina Tradicional da Mongólia , Plantas Medicinais/crescimento & desenvolvimento , Conservação dos Recursos Naturais/métodos , Meio Ambiente , Mongólia , Plantas Medicinais/classificação
9.
Nanoscale ; 5(5): 1980-5, 2013 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-23364424

RESUMO

Phosphorus (P)-doped nanogranular SiO(2) films are deposited by plasma-enhanced chemical vapor deposition at room temperature, and a high proton conductivity of ~5.6 × 10(-4) S cm(-1) is measured at room temperature with a relative humidity of 70%. The accumulation of protons at the SiO(2)/indium-zinc-oxide (IZO) interface induces a large electric-double-layer (EDL) capacitance. Thin-film transistors (TFTs) with two in-plane gates are self-assembled on transparent conducting glass substrates. The large EDL capacitance can effectively modulate the IZO channel with a current ON/OFF ratio of >10(7). Such TFTs calculate dual input signals at the gate level coupled with a floating gate, analogous to that of neuron MOS (vMOS). AND logic is demonstrated on the neuron TFTs. Such neuron TFTs gated by P-doped nanogranular SiO(2) shows an effective electrostatic modulation on conductivities of oxide semiconductors, which is meaningful for portable chemical-biological sensing applications.

10.
Vascul Pharmacol ; 47(2-3): 131-8, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17581794

RESUMO

Artesunate (ART), a semi-synthetic derivative of artemisinin extracted from the Chinese herb Artemisia annua, is a safe and effective antimalarial drug. In the present investigation, we analyzed the inhibitory effects of ART on angiogenesis and on VEGF production in chronic myeloid leukemia (CML) K562 cells in vitro and in vivo. In order to analyze the effect of ART on VEGF secretion in K562 cells, we examined the level of VEGF secreted in conditioned media (CM) by ELISA assay. The result showed that ART could decrease the VEGF level in CM of K562 cells, even at a lower concentration (2 micromol/l, P<0.01). The inhibitory effect of in vitro angiogenesis was tested on aortic sprouting in fibrin gel. ART could effectively suppress the stimulating angiogenic ability of CM by pretreated with K562 cells for 48 h in a time-dependent manner (days 3-14). The antiangiogenic effect of ART was further evaluated in vivo in chicken chorioallantoic membrane (CAM) neovascularization model. The result indicated that the stimulating angiogenic activity was decreased in response to the K562 cells treated with ART or the CM from K562 cells pretreated with ART in a dose-dependent manner (3-12 micromol/l). Furthermore, we analyzed the level of VEGF expression by western blot and detected the form of VEGF mRNA by RT-PCR in K562 cells. The experiments showed that ART could inhibit the VEGF expression, correlated well with the level of VEGF secreted in CM. These findings suggest that ART might present potential antileukemia effect as a treatment for CML therapy, or as an adjunct to standard chemotherapeutic regimens.


Assuntos
Inibidores da Angiogênese/farmacologia , Artemisininas/farmacologia , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Neovascularização Patológica , Sesquiterpenos/farmacologia , Fator A de Crescimento do Endotélio Vascular/efeitos dos fármacos , Inibidores da Angiogênese/administração & dosagem , Animais , Artemisia annua/química , Artemisininas/administração & dosagem , Artesunato , Embrião de Galinha , Relação Dose-Resposta a Droga , Regulação para Baixo , Humanos , Células K562 , Leucemia Mielogênica Crônica BCR-ABL Positiva/genética , Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia , Masculino , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sesquiterpenos/administração & dosagem , Fator A de Crescimento do Endotélio Vascular/metabolismo
11.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 28(2): 124-8, 2006 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16733889

RESUMO

OBJECTIVE: To identify the susceptibility genes of type 2 diabetes in Chinese Han population. METHODS: Single nucleotide polymorphism (SNP) discovery, genotyping and haplotype construction were performed in 30 candidate genes. Case-control study were carried out in a population-based sample and confirmed by the transmission disequilibrium test (TDT) analysis in 77 trio pedigrees. The effects of the SNP rs5210 on gene expression were studied by reporter gene technique. RESULTS: The case-control studies showed that several SNPs on KCNJ11 gene was associated with type 2 diabetes in Chinese Han population, in which the allele frequency of SNP rs5219, the genotype frequency of rs5210, rs2285676 and rs5219, and the frequency of haplotype GA combined of the rs5219 and rs5215 showed significant difference between these two groups (P < 0.05). In addition, TDT test also showed statistical significance on this haplotype GA (P < 0. 05). The reporter gene assay showed that the effect on gene expression was significantly different between two alleles of rs5210 (P < 0.05). CONCLUSION: KCNJII gene is one of the susceptibility genes of type 2 diabetes in Chinese Han population.


Assuntos
Diabetes Mellitus Tipo 2/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Feminino , Testes Genéticos , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade
12.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 27(3): 274-9, 2005 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16038259

RESUMO

OBJECTIVE: To search for the susceptibility variant (s) of type 2 diabetes in the susceptible regions on chr.1 (1p36.23-36.33, 1q24.3-25.1, and 1q42.12-42.13) by genotyping SNP markers in case-control DNA samples and identifying the haplotype associated with type 2 diabetes. METHODS: Totally 124 SNPs in 33 candidate genes in the mapped regions were chosen from public SNP data or identified by sequencing the samples that were used to search for SNP locus. Sequencing method was used to genotype the loci for 236 sporadic type 2 diabetes patients and 152 normal subjects in Northern Han Chinese population. The haplotypes with significant difference were further analyzed. RESULTS: Of 124 SNPs successfully typed, 4 SNPs that showed association with diabetes status were found: rs203849 (P=0.005, OR=1.60) and rs203826 (P=0.016, OR=1.60) located in sAC gene, rs7535528 (P=0.028, OR=1.45) located in PANK4, rs884363 (P=0.043, OR=1.37) located in CASP9 gene. In addition, the frequencies of two combination types from these 4 SNP genotypes were significantly different between case and control groups (P < 0.001). Furthermore, four haplotypes associated with diabetes were found in haplotype analysis of sAC gene. CONCLUSION: sAC, PANK4, and CA SP9 may be associated with type 2 diabetes in Han population in north China, and it seems that the synergetic effect of these genes is responsible for the development of type 2 diabetes.


Assuntos
Diabetes Mellitus Tipo 2/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Adulto , Apoptose/genética , Caspase 9 , Caspases/genética , China , Cromossomos Humanos Par 1/genética , Feminino , Testes Genéticos , Variação Genética , Genótipo , Haplótipos/genética , Humanos , Masculino , Pessoa de Meia-Idade
13.
Cancer Chemother Pharmacol ; 53(5): 423-32, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15132130

RESUMO

Dihydroartemisinin, a more water-soluble metabolite of artemisinin derivatives, is a safe and most effective antimalarial analog of artemisinin. In the present study, we investigated the antiangiogenic activity of dihydroartemisinin in vitro and in vivo, and investigated dihydroartemisinin-induced apoptosis in human umbilical vein endothelial cells (HUVEC). Dihydroartemisinin markedly reduced VEGF binding to its receptors on the surface of HUVEC. The expression levels of two major VEGF receptors, Flt-1 and KDR/flk-1, on HUVEC were lower following dihydroartemisinin treatment as shown by an immunocytochemical staining assay. The in vivo antiangiogenic activity was evaluated in the model of chicken chorioallantoic membrane (CAM) neovascularization. Dihydroartemisinin significantly inhibited CAM angiogenesis at low concentrations (5-30 nmol/100 microl per egg). We also investigated both qualitatively and quantitatively the induction of HUVEC apoptosis by dihydroartemisinin. A dose-related (5-80 microM) and time-dependent (6-36 h) increase in dihydroartemisinin-induced HUVEC apoptosis was observed by flow cytometry. Our results suggest that the antiangiogenic effect induced by dihydroartemisinin might occur by induction of cellular apoptosis and inhibition of expression of VEGF receptors. These findings and the known low toxicity of dihydroartemisinin indicate that it might be a promising candidate angiogenesis inhibitor.


Assuntos
Inibidores da Angiogênese/farmacologia , Antimaláricos/farmacologia , Artemisininas/farmacologia , Sesquiterpenos/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Citometria de Fluxo , Humanos , Marcação In Situ das Extremidades Cortadas , Microscopia de Fluorescência , Receptores de Fatores de Crescimento do Endotélio Vascular/antagonistas & inibidores , Fator A de Crescimento do Endotélio Vascular/metabolismo
14.
Pharmacology ; 71(1): 1-9, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15051917

RESUMO

Artesunate (ART) is a semi-synthetic derivative of artemisinin extracted from the plant Artemisia annua is a safe and effective antimalarial drug. In the present investigation, ART was found also to inhibit angiogenesis in vivo and in vitro. The anti-angiogenic effect in vivo was evaluated in nude mice by means of human ovarian cancer HO-8910 implantation and immunohistochemical stainings for microvessel (CD(31)), vascular endothelial growth factor (VEGF) and VEGF receptor KDR/flk-1. Tumor growth was decreased and microvessel density was reduced following drug treatment with no apparent toxicity to the animals. ART also remarkably lowered VEGF expression on tumor cells and KDR/flk-1 expression on endothelial cells as well as tumor cells. The in vitro effect of ART was tested on models of angiogenesis, namely, proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVEC). The results showed that ART significantly inhibited angiogenesis in a dose-dependent form in the range of 0.5 approximately 50 micromol/l. Additionally, the inhibitory effect of ART on HVUEC proliferation was stronger than that on Hela, JAR, HO-8910 cancer cells, NIH-3T3 fibroblast cells and human endometrial cells, indicating that ART was selectively against HUVEC. These findings and the known low toxicity of ART are clues that ART may be a promising angiogenesis inhibitor.


Assuntos
Inibidores da Angiogênese/farmacologia , Artemisininas/farmacologia , Neovascularização Fisiológica/efeitos dos fármacos , Sesquiterpenos/farmacologia , Fator A de Crescimento do Endotélio Vascular/biossíntese , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/biossíntese , Animais , Antimaláricos/administração & dosagem , Antimaláricos/farmacologia , Artemisininas/administração & dosagem , Artemisininas/síntese química , Artesunato , Peso Corporal/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Feminino , Humanos , Injeções Subcutâneas , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Invasividade Neoplásica/prevenção & controle , Transplante de Neoplasias , Neoplasias Ovarianas/patologia , Sesquiterpenos/administração & dosagem , Sesquiterpenos/síntese química , Veias Umbilicais/citologia , Veias Umbilicais/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/efeitos dos fármacos , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
15.
Vascul Pharmacol ; 41(6): 205-12, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15653096

RESUMO

Artesunate (ART), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua, is an effective novel antimalarial drug. The present study investigated the apoptotic activity of artesunate in cultured human umbilical vein endothelial cell (HUVEC) by means of nuclear staining, DNA agarose gel electrophoresis, and flow cytometry. The observations also indicated that artesunate induced apoptosis of HUVEC in a concentration-dependent and time-dependent manner. A Western immunoblot analysis showed down-regulation of the bcl-2 protein and up-regulation of the bax protein in the artesunate-treated HUVEC. Ca2+ in cells was evaluated by fluorescent spectrophotometer using Fura 2-AM as probe. These results suggest that artesunate may be a potential apoptosis-inducing agent for endothelial cells.


Assuntos
Apoptose/efeitos dos fármacos , Artemisininas/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Células Endoteliais/efeitos dos fármacos , Sesquiterpenos/farmacologia , Veias Umbilicais/efeitos dos fármacos , Artemisia annua , Artesunato , Sinalização do Cálcio/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Células Endoteliais/citologia , Regulação da Expressão Gênica , Humanos , Recém-Nascido , Proteínas Proto-Oncogênicas c-bcl-2/efeitos dos fármacos , Veias Umbilicais/citologia , Proteína X Associada a bcl-2
16.
World J Gastroenterol ; 9(9): 2078-82, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12970910

RESUMO

AIM: To identify the susceptible gene (s) for type 2 diabetes in the previously mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes. METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed. RESULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/zetagene (PRKCZ) was statistically different between the case and control groups(P<0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified. CONCLUSION: PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Diabetes Mellitus Tipo 2/genética , Haplótipos , Proteína Quinase C/genética , Estudos de Casos e Controles , China/etnologia , Frequência do Gene , Humanos , Polimorfismo de Nucleotídeo Único
17.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 24(3): 223-7, 2002 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-12905622

RESUMO

OBJECTIVE: To probe the candidate susceptibility gene (s) of type 2 diabetes in the formal mapping region, 1p36.33-p36.23, in Han people of Northern China using single nucleotide polymorphisms (SNPs). METHODS: 23 SNPs located in 10 candidate genes in the mapping region were chosen from public SNP domain by bioinformatic methods and single base extension (SBE) method were used to genotype the loci in 192 sporadic type 2 diabetes patients and 172 normal individuals to perform case-control study. RESULTS: Among the 23 SNPs, 8 were found to be common in Chinese population. There were statistically different in the allele frequency of 2 SNP, rs436045 in the protein kinase C/xi gene and rs228648 in Urotensin II gene between case and control groups. CONCLUSIONS: The two SNP may be associated with type 2 diabetes in Han people of China, which makes base for further study of the relation between the genes they located with type 2 diabetes.


Assuntos
Diabetes Mellitus Tipo 2/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Proteína Quinase C/genética , Urotensinas/genética , Alelos , Estudos de Casos e Controles , Grupos Étnicos , Testes Genéticos , Genótipo , Humanos
18.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 24(5): 471-3, 2002 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-12905767

RESUMO

OBJECTIVE: To study the function of 5 single nucleotide polymorphisms (SNPs) of the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, in the pathogenesis of the disease. METHODS: Bioinformatic methods and reporter gene activity determination were used to analyze the function of the 5 SNPs. RESULTS: The reporter gene activities of different alleles of 2 SNPs, rs427811 and rs809912, were obviously different, which implies that these 2 SNPs might be susceptibility loci of the disease. CONCLUSION: The PRKCZ gene is further confirmed to be a susceptibility gene for type 2 diabetes in Han population of North China. Two SNPs in the gene play a role in the pathogenesis of the disease by affecting the expression level of PRKCZ gene.


Assuntos
Diabetes Mellitus Tipo 2/genética , Polimorfismo de Nucleotídeo Único , Proteína Quinase C/genética , Alelos , Grupo com Ancestrais do Continente Asiático , Grupos Étnicos , Predisposição Genética para Doença , Humanos , Proteína Quinase C-delta
19.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 24(5): 474-80, 2002 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-12905768

RESUMO

OBJECTIVE: To search for the disease-associated haplotype in the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, by case-control study and linkage disequilibrium (LD) analysis using single nucleotide polymorphisms (SNPs). METHODS: SNPs located in the PRKCZ gene were chosen from public SNP domain by bioinformatic methods and single base extension (SBE) method was used to genotype the loci in 173 sporadic type 2 diabetes patients and 152 normal individuals to perform case-control study and LD analysis. Haplotype block were constructed in these populations. RESULTS: Several SNPs in the PRKCZ gene were found to be associated with the disease. The SNPs formed different haplotype block pattern in case and control groups. The frequencies of the haplotypes formed by 5 SNPs were statistically different between the two groups. CONCLUSION: The haplotype formed by 5 SNPs in the PRKCZ gene may be associated with type 2 diabetes in Han population of China, which is confirmed from statistics to be a susceptibility gene for the disease.


Assuntos
Diabetes Mellitus Tipo 2/genética , Desequilíbrio de Ligação , Polimorfismo de Nucleotídeo Único , Proteína Quinase C/genética , Alelos , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , Grupos Étnicos , Predisposição Genética para Doença , Haplótipos , Humanos , Proteína Quinase C-delta
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