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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 266: 120416, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34600321

RESUMO

Sr3LiSbO6 phosphors were prepared by high temperature solid state reaction method. The crystal phase, morphology and optical properties were characterized by X-ray powder diffraction spectroscopy, scanning electronic microscope, absorption and photoluminescence (PL) spectra. The XRD Rietveld refinement was performed to obtain the detailed crystal structure of Sr3LiSbO6. The electronic structure was analyzed by density functional theory (DFT) calculation. Sr3LiSbO6 possessed indirect band structure and the band-gap were determined to be 3.17 eV. Self-activated far-red emissions at 630-800 nm were detected under the excitation at 340 nm, which was proposed to originate from the transition between interstitial oxygen defective state to six hybrid 4d105s0 states of Sb5+ according to the results of PL spectra of samples annealed at different atmospheres. The PL intensity can be significantly enhanced by 2.9 times after doping 2 mol% Gd3+ ions in Sr3LiSbO6. The internal quantum efficiency of Sr3LiSbO6:2 mol%Gd3+ was determined to be 25.2%. The influence of the Gd3+ doping on the self-activated PL lifetimes of Sr3LiSbO6 and the thermal quenching property of Sr3LiSbO6:2 mol%Gd3+ was studied.


Assuntos
Oxigênio
2.
Sci Total Environ ; 804: 150293, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34798762

RESUMO

Molecularly imprinted polymers (MIPs) have added a vital contribution to food quality and safety with the effective extraction of pesticide residues due to their unique properties. Magnetic molecularly imprinted polymers (MMIPs) are a superior approach to overcome stereotypical limitations due to their unique core-shell and novel composite structure, including high chemothermal stability, rapid extraction, and high selectivity. Over the past two decades, different MMIPs have been developed for pesticide extraction in actual food samples with a complex matrix. Nevertheless, such developments are desirable, yet the synthesis and mode of application of MMIP have great potential as a green chemistry approach that can significantly reduce environmental pollution and minimize resource utilization. In this review, the MMIP application for single or multipesticide detection has been summarized by critiquing each method's uniqueness and efficiency in real sample analysis and providing a possible green chemistry exploration procedure for MMIP synthesis and application for escalated food and environmental safety.


Assuntos
Impressão Molecular , Resíduos de Praguicidas , Fenômenos Magnéticos , Magnetismo , Polímeros Molecularmente Impressos , Resíduos de Praguicidas/análise , Extração em Fase Sólida
3.
Front Psychiatry ; 12: 692266, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721096

RESUMO

It has been suggested that the altered function of reward and punishment is an important vulnerability factor leading to the development of drug use disorders. Previous studies have identified evidence of neurophysiological dysfunction in the reward process of individuals with substance use disorders. To date, only a few event-related potential (ERP) studies have examined the neural basis of reward and punishment processing in women with methamphetamine (MA) use disorders. The current ERP research aims to investigate the neurophysiological mechanisms of reward and punishment in women with MA use disorder using a monetary incentive delay task. Nineteen women with MA use disorder (MA group) and 20 healthy controls (HC group) were recruited in this study. The behavioral data showed that the reaction time (RT) was faster and the response accuracy (ACC) was higher for the potential reward and punishment conditions compared to neutral conditions. During the monetary incentive anticipation stage, the Cue-P3, and stimulus-preceding negativity (SPN) were larger in the MA group than in the HC group. The SPN under the potential reward condition was larger than that under the neutral condition in the MA group but not in the HC group. During the monetary incentive consummation stage, the feedback-related negativity and feedback P3 (FB-P3) following positive feedback were significantly larger than negative feedback in the potential reward condition for the HC group, but not for the MA group. However, the FB-P3 following negative feedback was significantly larger than positive feedback in the potential punishment condition for the MA group, but not the HC group. The results suggest that women with MUD have stronger expectations of generic reward and stronger response of generic harm avoidance, which could be targeted in designing interventions for women with MA use disorder.

4.
Nat Commun ; 12(1): 6327, 2021 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-34732696

RESUMO

Osteoclasts (OCs), the only cells capable of remodeling bone, can demineralize calcium minerals biologically. Naive OCs have limitations for the removal of ectopic calcification, such as in heterotopic ossification (HO), due to their restricted activity, migration and poor adhesion to sites of ectopic calcification. HO is the formation of pathological mature bone within extraskeletal soft tissues, and there are currently no reliable methods for removing these unexpected calcified plaques. In the present study, we develop a chemical approach to modify OCs with tetracycline (TC) to produce engineered OCs (TC-OCs) with an enhanced capacity for targeting and adhering to ectopic calcified tissue due to a broad affinity for calcium minerals. Unlike naive OCs, TC-OCs are able to effectively remove HO both in vitro and in vivo. This achievement indicates that HO can be reversed using modified OCs and holds promise for engineering cells as "living treatment agents" for cell therapy.

5.
Thorac Cancer ; 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34741433

RESUMO

OBJECTIVE: To evaluate the diagnostic performance of short state homeobox 2 (SHOX2) promoter methylation as biomarker for lung cancer identification through aggregating the open published data. METHODS: We did an electronic search in Pubmed, EMBASE, Ovid, Web of Science, Google Scholar, and the China National Knowledge Infrastructure (CNKI) to identify the publications related to SHOX2 promoter methylation and lung cancer. The diagnostic performance of sensitivity (SEN), specificity (SPE), odds ratio (DOR), and summary receiver operating characteristic (SROC) cure were aggregated by fixed or random effect model. Fagan's nomogram was used to investigate the post-test diagnostic probability. Deek's funnel plot and line regression test was applied to evaluate the publication bias. RESULTS: In total, 18 clinical studies about SHOX2 promoter methylation and lung cancer were included in the meta-analysis. The combined diagnostic SEN, SPE, DOR were 0.63 (95% CI = 0.54-0.70), 0.91 (95% CI = 0.87-0.94), and 16.84 (95% CI = 11.18-25.36) in random effect model respectively. The pooled area under the curve (AUC) of SROC was 0.88 (95% CI = 0.84-0.90). The post-test probability of lung cancer was 88% and 29% when SHOX2 methylated and unmethylated in humoral components given a pre-test probability of 50%. Deek's funnel plot and regression test indicated no publication bias (p = 0.62). CONCLUSION: SHOX2 promoter methylation in humoral components may be a potential biomarker for lung cancer diagnosis with relative high diagnostic specificity.

6.
Front Hum Neurosci ; 15: 728405, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34751217

RESUMO

Objective: Rapid eye movement (REM) sleep behavior disorder (RBD) is a disease characterized by dream enacting behavior and is now commonly believed to be a harbinger to alpha-synucleinopathy diseases such as dementia with Lewy bodies, Parkinson's disease, and multiple system atrophy. The aim of this study was to explore the quasi-stable topological structure of the brain in RBD by analyzing resting-state electroencephalography (EEG) microstates. Methods: We enrolled 22 participants with RBD and 46 healthy controls (HCs) with age and gender-matched. After the resting-state EEG recordings were acquired, EEG microstate features were analyzed to assess the functional networks of all participants. Results: Significant differences in the brain topological structure and temporal characteristics of sub-second brain activity were identified between the RBD and HCs. The RBD group had a shorter average duration of microstate A and microstate D when compared with HCs, and microstate B contributed more, while microstate D contributed significantly less to the RBD group. Furthermore, the average duration and proportion of microstate D were negatively correlated with the RBD questionnaire Hong Kong (RBDQ-HK) score. Conclusion: The result of this study indicates that the microstate dynamics is disturbed in RBD, which might jeopardize the flexibility and adaptability of the brain. Microstates are potential biomarkers to explore the early electrophysiological abnormality of alpha-synucleinopathy diseases.

7.
Pers Individ Dif ; 180: 110973, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34629580

RESUMO

With the worldwide implementation of quarantine regulations to suppress the spread of the COVID-19, anxiety, interpersonal distancing and autistic tendency may decrease individuals' desire to seek interpersonal information and thus might have negative effects on their interpersonal curiosity. Through behavioral paradigms and scales, two studies were conducted (Study 1: n = 570; Study 2: n = 501). We explored the predictive effect of anxiety on interpersonal curiosity in situations when mandatory isolation measures have led to dramatic changes in interpersonal distancing and autistic tendency. We found that interpersonal distancing and autistic tendency negatively predicted interpersonal curiosity, and these predictive effects suppressed the positive prediction of state anxiety to interpersonal curiosity. Our research provides insights into the relationships among anxiety, curiosity, interpersonal distancing, and autistic tendency during the COVID-19 pandemic.

8.
Artigo em Inglês | MEDLINE | ID: mdl-34672662

RESUMO

The congruence effect can be modulated by adjacent conflict conditions, producing the congruency sequence effect (CSE). However, many boundary conditions prevent the transfer of the cross-conflict CSE. A consensus has been achieved that the CSE reflects both top-down control and bottom-up associative learning, but neither perspective could perfectly interpret the various boundary conditions. Their imperfections recently inspired an integrative learning account of cognitive control, which predicts that conflict similarity affects the magnitude of the cross-conflict CSE. We examined this hypothesis with the spatial Stroop-Simon paradigm by introducing a compound condition containing both the Stroop and Simon components (Experiment 1). The conflict similarity was defined by the degree of component overlap, as manipulated by the polar angle of the target arrow in Experiments 2a and 2b and by the Euclidean distance of the target arrow in Experiments 3a and 3b. Mixed-effect modeling analyses indicated that, in all experiments, the cross-conflict CSEs were positively correlated with the similarity among conflict conditions. Specifically, the compound condition with equal Stroop and Simon components generated comparable CSEs with both the Stroop and Simon conditions (Experiment 1). When the compound condition was more similar to the Stroop than the Simon condition, a trend of a larger CSE was observed between the compound conflict and the Stroop condition than between the compound conflict and the Simon condition, and vice versa (Experiments 2 and 3). Our study revealed that the continuum of the cross-conflict CSE was modulated by conflict similarity, hence supporting the integrative learning account of cognitive control. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

9.
Chem Sci ; 12(34): 11576-11584, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34567505

RESUMO

The severe degradation of Fe-N-C electrocatalysts during a long-term oxygen reduction reaction (ORR) has become a major obstacle for application in proton-exchange membrane fuel cells. Understanding the degradation mechanism and regeneration of aged Fe-N-C catalysts would be of particular interest for extending their service life. Herein, we show that the by-product hydrogen peroxide during the ORR not only results in the oxidation of the carbon surface but also causes the demetallation of Fe active sites. Quantitative analysis reveals that the Fe demetallation constitutes the main reason for catalyst degradation, while previously reported carbon surface oxidation plays a minor role. We further reveal that post thermal annealing of the aged catalysts can transform the oxygen functional groups on the carbon surface into micropores. These newly formed micropores not only help to increase the active-site density but also the intrinsic ORR activity of the neighbouring Fe-N4 sites, both contributing to complete activity recovery of aged Fe-N-C catalysts.

10.
Antonie Van Leeuwenhoek ; 114(10): 1541-1549, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34401954

RESUMO

An obligate anaerobic bacterial BAD-10 T was isolated from anaerobic acetochlor-degrading sludge. The strain was Gram-stain negative, curved rod-shaped, non-motile and non-spore-forming. Growth was observed in PYT medium at pH 6.0-9.0 (optimum, pH 7.5), at 25-47 °C (37 °C) and with 0-1.0% NaCl (w/v, 0%). Strain BAD-10 T could degrade acetochlor. The major fermentation products from peptone-yeast (PY) medium were acetate and butyrate. The predominant cellular fatty acids were iso-C15:0 FAME, anteiso-C15:0 FAME and C16:0 FAME. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain BAD-10 T showed closest affiliation to Proteiniclasticum ruminis D3RC-2 T, with a sequence similarity of 97.6%. Genome sequencing revealed a genome size of 2,983,986 bp, a G + C content of 51.4 mol% and protein-coding genes of 3,102. The average nucleotide identity and in silico DNA-DNA hybridization values between strain BAD-10 T and Proteiniclasticum ruminis D3RC-2 T were 71.0% and 20.4%, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, physiological and phylogenetic evidence, strain BAD-10 T represents a novel species in the genus Proteiniclasticum, for which the name Proteiniclasticum sediminis sp. nov. is proposed. Strain BAD-10 T (= CCTCC AB 2021091 T = KCTC 25288 T) is the type strain of the proposed novel species.


Assuntos
Ácidos Graxos , Esgotos , Anaerobiose , Técnicas de Tipagem Bacteriana , Composição de Bases , Clostridiaceae , DNA Bacteriano/genética , Hibridização de Ácido Nucleico , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
Dalton Trans ; 50(35): 12137-12146, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34396381

RESUMO

In this article, we synthesized a series of new warm-white emitting persistent luminescent phosphors by co-doping Zn2+ into Pr3+ activated BaLu2Al2Ga2SiO12, and systematically investigated the effect of Zn2+ co-doping on both their photoluminescence and persistent luminescence properties. Following the removal of UV excitation, the phosphor emits warm-white persistent luminescence consisting of greenish-blue and red emissions originating from 3P0 and 1D2 multiplet electron transitions at the 4f level of Pr3+. The luminescence properties of the Ba1-xZnxLu2Al2Ga2SiO12:Pr3+ phosphors can be modified by changing the content of Ba/Zn in the host, which affects the non-radiative energy flow between 5d1-3P0-1D2 levels and resultantly enhances the intensity of the 4f → 4f transition. Compared with the undoped sample, Zn2+ co-doping can significantly enhance the persistent luminescence intensity of the phosphors in the range of 400-800 nm and reduce the intensity in the UV region. Meanwhile, Zn2+ co-doping can also change the intensity ratio between the greenish-blue and red emissions, and the persistent luminescence color can be tuned from red to warm-white with the increase of Zn2+ concentration. Besides, the Zn2+ ions entering the crystal lattice also enhance the persistent luminescence performance by modifying the defect levels in the phosphor. For the optimized phosphor, bright warm-white persistent luminescence can be observed by the naked eye in the dark after the removal of the excitation source for 4 h. Based on the experimental results, a feasible mechanism was also proposed to reveal the persistent luminescence generation process for the BaLu2Al2Ga2SiO12:Pr3+,Zn2+ phosphor.

12.
Medicine (Baltimore) ; 100(29): e26654, 2021 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-34398024

RESUMO

RATIONALE: Anti leucine-rich glioma inactivated 1 (LGI1) limbic encephalitis (LE) is rare autoimmune encephalitis, characterized by acute or subacute cognitive impairment, faciobrachial dystonic seizures, mental disorders, and refractory hyponatremia. As a type of treatable rapidly progressive dementia with a good prognosis, early, and accurate diagnosis is essential. We present a case of anti-LGI1 LE who was initially misdiagnosed with Alzheimer disease because his clinical manifestations were similar to Alzheimer disease. PATIENT CONCERNS: A male patient presenting with rapidly progressive dementia, faciobrachial dystonic seizures, psychiatric disturbance, and refractory hyponatremia was admitted. The scores of Mini-Mental State Examination, Montreal Cognitive Assessment, and Neuropsychiatric Inventory were 19/30, 16/30, and 91/144, respectively. Brain magnetic resonance images indicated moderate atrophy of the hippocampus and abnormally hyperintensities in the left medial temporal and hippocampus. DIAGNOSIS: The patient was diagnosed with anti-LGI1 LE based on the presence of LGI-1 antibodies in the cerebrospinal fluid and serum and clinical manifestations. INTERVENTIONS: Patient was treated with glucocorticoid against LGI1, antiepileptic drug, cholinesterase inhibitors, and other adjuvant therapy. OUTCOMES: The patient showed marked improvement on immunotherapy. Clinical symptoms were disappeared and the LGI-1 antibodies in cerebrospinal fluid and serum were both negative at the time of discharge. CONCLUSIONS: Recognition of the specific symptoms and LGI-1 antibody test will be helpful for the early diagnosis, prompt immunotherapy, and good prognosis. This case raises the awareness that rapidly progressive dementia with frequent seizures could be caused by immunoreactions.


Assuntos
Encefalite Límbica/diagnóstico , Idoso , Autoanticorpos/sangue , Autoanticorpos/líquido cefalorraquidiano , Demência/etiologia , Diagnóstico Diferencial , Humanos , Encefalite Límbica/complicações , Encefalite Límbica/diagnóstico por imagem , Encefalite Límbica/imunologia , Imageamento por Ressonância Magnética , Masculino , Convulsões/etiologia
13.
Front Public Health ; 9: 707435, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34458227

RESUMO

Acinetobacter baumannii is an important nosocomial pathogen, which is multidrug resistant (MDR). Acinetobacter baumannii has become a major threat to public health worldwide due to its ability to easily acquire resistant genes. In order to analyze its epidemiology characteristics and the genetic evolution, A. baumannii isolates obtained from a Chinese tertiary hospital in the past 12 years (2008-2019), 295 isolates of non-repetitive A. baumannii, were recovered from patients and wards environments. The resistance genes were analyzed using antimicrobial susceptibility testing. The genetic relatedness of 295 isolates was identified by multilocus sequence typing (MLST) and eBURST analysis. It was found that the antibiotic-resistant and carbapenemase-resistant genes of all the 295 MDR A. baumannii in the hospital have not changed significantly over the past 12 years; all of them were resistant to multiple antibiotics except the polymyxin E and tigecycline. The results of drug-resistant genes showed that the detection rates of carbapenemase-resistant genes bla OXA-23, bla TEM-1, and bla OXA-66 were 97.6, 75.3, and 71.9%, respectively, which were detected almost every year from 2008 to 2019. Additionally, 16s rRNA methylation enzyme gene armA, aminoglycoside-resistant gene ant(3")-I, and class I integrase gene could also have a high positive rate. By MLST, these isolates were assigned to 12 sequence types (STs), including ST369, ST208, ST195, ST191, ST368, ST530, ST469, ST451, ST229, ST381, ST543, and ST1176. eBURST analysis showed that 9 STs with ST208 as the founder genotype belonged to Group 1 except for ST229, ST530, and ST1176. Therefore, most MDR A. baumannii isolates had a relatively close genetic relationship. Notably, the predominant ST208 and ST369 at the early stage changed to ST451 in 2019, indicating that the complex and diverse genetic background of the prevalence of A. baumannii isolates in the hospital. Overall, further epidemiological surveillance and genetic evolution analysis of A. baumannii are required, which can provide new strategies for the prevention and control of A. baumannii infections.


Assuntos
Infecções por Acinetobacter , Acinetobacter baumannii , Infecções por Acinetobacter/tratamento farmacológico , Acinetobacter baumannii/genética , China/epidemiologia , Farmacorresistência Bacteriana Múltipla/genética , Humanos , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , RNA Ribossômico 16S , Centros de Atenção Terciária
14.
BMC Med Genomics ; 14(1): 196, 2021 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-34330286

RESUMO

BACKGROUND: Inherited hypertrophic cardiomyopathy (HCM) is a common heart muscle disease that damages heart function and may cause the heart to suddenly stop beating. Genetic factors play an important role in HCM. Pedigree analysis is a good way to identify the genetic defects that cause disease. METHODS: An HCM pedigree was determined in Yunnan, China. Whole-exome sequencing was performed to identify the genetic variants of HCM. Another 30 HCM patients and 200 healthy controls were also used to investigate the frequency of the variants by customized TaqMan genotyping assay. RESULTS: The variant NM_000257.4:c.3134G > A (NP_000248.2:p.Arg1045His, rs397516178, c.3134G > A in short) was found to cosegregate with the clinical phenotype of HCM. Moreover, the variant was not found in the 200 control subjects. After genotyping the variant in 30 HCM patients, there was one patient who carried the variant and had a family history. CONCLUSIONS: Our findings suggest that this variant may be closely related to the occurrence of the disease. According the ACMG guidelines, the c.3134G > A variant should be classified as "Likely pathogenic".

15.
Int J Nanomedicine ; 16: 4863-4871, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34295159

RESUMO

Background: Phototherapy has significant potential as an effective treatment for cancer. However, the application of a multifunctional nanoplatform for photodynamic therapy (PDT) and photothermal therapy (PTT) at a single excitation wavelength remains a challenge. Materials and Methods: The double emulsion solvent evaporation method was used to prepare toluidine blue@poly lactic-co-glycolic acid (TB@PLGA) nanoparticles (NPs). The biocompatibility of TB@PLGA NPs was evaluated, and a 660 nm luminescence was used as the light source. The photothermal effect, photothermal stability, and singlet oxygen yield of NPs in an aqueous solution verified the feasibility of NPs as a PTT/PDT synergistic therapy drug. Results: TB@PLGA NPs were successfully prepared and characterized. In vitro experiments demonstrated that TB@PLGA NPs can cause massive necrosis of tumor cells and induce apoptosis through a photodynamic mechanism under 660 nm laser irradiation. The TB@PLGA NPs also achieved optimal tumor inhibition effect in vivo. Conclusion: The TB@PLGA NPs prepared in this study were applied as a dual-mode phototherapeutic agent under single laser irradiation. Both in vitro and in vivo experiments demonstrated the good potential of PTT/PDT for tumor inhibitors.


Assuntos
Nanopartículas , Neoplasias , Linhagem Celular Tumoral , Glicóis/uso terapêutico , Humanos , Neoplasias/tratamento farmacológico , Fotoquimioterapia , Fototerapia , Cloreto de Tolônio
16.
Int J Gen Med ; 14: 3485-3496, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34290523

RESUMO

Background: Topoisomerase 2-alpha (TOP2A) has been identified as a hub gene that played an important role in the initiation and progression of thyroid carcinoma (THCA). However, the exact function of TOP2A in papillary thyroid cancer (PTC) remained elusive. The current study aimed to evaluate the TOP2A expression, prognosis significance and key signaling pathways involved in PTC. Methods: We firstly evaluated the expression of TOP2A in PTC via UALCAN, cBioportal, HPA and LinkdedOmics databases. Genetic alteration of TOP2A in PTC was then explored in cBioportal. Prognostic impacts of TOP2A expression on disease-free survival (DFS) of PTC patients were subsequently evaluated using Kaplan-Meier plotter and Gepia databases. Taking gender, age, cancer stage, T, N and M stages into consideration, we compared survival difference between TOP2A high and low expression groups. KEGG pathway analysis in WebGestalt and GSEA analysis were further performed to reveal the potential TOP2A-associated signaling pathways involved in PTC. Finally, the upstream microRNAs of TOP2A were assessed using DIANA, TargetScan, miRDB and miRWALK database, followed by mechanism exploration of upstream microRNAs. Results: 1) The mRNA and protein of TOP2A were highly expressed in PTC tissue compared with normal thyroid tissue. TOP2A expression was associated with patient's age, N stage and cancer stage (all P<0.05). TOP2A protein was mainly localized to nucleoplasm. 2) Most of samples occurred the missense substitution, and mutation site was located at K1199E. Nucleotide mutations were mainly presented as G>A (35.29%). 3) TOP2A high expression significantly influenced the DFS of PTC patients (P=0.015). Restricted survival analysis showed that TOP2A high expression caused poorer DFS of female patients (P=0.003) and those with age <60 years old (P=0.002), early clinical stage (P=0.012), N0 stage (P=0.002) or M0 stage (P=0.040). 4) Pathway analysis suggested that TOP2A positively participated in the cell cycle, oocyte meiosis and p53 signaling pathways (all P<0.05) involved in thyroid cancer. Conclusion: The expression of TOP2A was higher in PTC tissue, which resulted in a worse DFS of patients with PTC. TOP2A might act as an effective therapeutic target for PTC treatment.

17.
Front Pediatr ; 9: 543078, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34307241

RESUMO

Background: Inflammatory myofibroblastic tumor (IMFT) is a rare neoplasm mainly affecting children and young adults. We conducted a retrospective study to evaluate the clinical features and treatment alternatives of childhood inflammatory myofibroblastic tumors. Methods: A total of 19 patients who were pathologically diagnosed with IMT between December 2008 and October 2018 were included. Collected data were demographic information, main complaints, tumor characteristics, treatment, pathological results, immunohistochemical analysis, and prognosis. Results: The male/female ratio was 13:6. The mean age at disease onset was 44.9 ± 33.9 months (range 4 to 111 months). The mean tumor size was 6.5 ± 4.0 cm (range 1.2 to 17.0 cm). The most common site was the abdomen (13/19). The most commonly used detection tool was CT. Eleven patients (57.9%) had aggressive tumor growth, including eight receiving extensive resection and three receiving palliative resection due to high local invasiveness and postoperative chemotherapy. Eight cases whose tumors were completely enveloped received complete resection. Immunohistochemistry was performed for 17 patients and ALK positivity was found in 11 patients. Despite three children lost to follow-up, sixteen patients were followed up for 6 to 132 months (average 63.9 months, median 66 months). Of which, twelve children survived with no evidence of IMT, and four cases (21%) showed local recurrences (two of them died). No distant metastasis was detected. Conclusions: IMT is rare in children with various locations, mostly appearing in the abdomen. Whether the tumor could be completely removed, the location and the invasiveness of surrounding tissues might be highly prognosis-related.

18.
NPJ Regen Med ; 6(1): 36, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34188056

RESUMO

Cardiovascular disease is the leading cause of death in the world due to losing regenerative capacity in the adult heart. Frogs possess remarkable capacities to regenerate multiple organs, including spinal cord, tail, and limb, but the response to heart injury and the underlying molecular mechanism remains largely unclear. Here we demonstrated that cardiomyocyte proliferation greatly contributes to heart regeneration in adult X. tropicalis upon apex resection. Using RNA-seq and qPCR, we found that the expression of Fos-like antigen 1 (Fosl1) was dramatically upregulated in early stage of heart injury. To study Fosl1 function in heart regeneration, its expression was modulated in vitro and in vivo. Overexpression of X. tropicalis Fosl1 significantly promoted the proliferation of cardiomyocyte cell line H9c2. Consistently, endogenous Fosl1 knockdown suppressed the proliferation of H9c2 cells and primary cardiomyocytes isolated from neonatal mice. Taking use of a cardiomyocyte-specific dominant-negative approach, we show that blocking Fosl1 function leads to defects in cardiomyocyte proliferation during X. tropicalis heart regeneration. We further show that knockdown of Fosl1 can suppress the capacity of heart regeneration in neonatal mice, but overexpression of Fosl1 can improve the cardiac function in adult mouse upon myocardium infarction. Co-immunoprecipitation, luciferase reporter, and ChIP analysis reveal that Fosl1 interacts with JunB and promotes the expression of Cyclin-T1 (Ccnt1) during heart regeneration. In conclusion, we demonstrated that Fosl1 plays an essential role in cardiomyocyte proliferation and heart regeneration in vertebrates, at least in part, through interaction with JunB, thereby promoting expression of cell cycle regulators including Ccnt1.

19.
Chemosphere ; 282: 130976, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34089999

RESUMO

Atrazine is one of the most widely used herbicides, however it and its metabolites cause widespread contamination in soil and ground water. Bioaugmentation is an effective method for remediation of environmental organic pollutants. High-throughput sequencing provides an important tool for understanding the changes of microbial community and function in response to pollutants degradation based on bioaugmentation. In this study, the effect of biodegradation with Paenarthrobacter sp. W11 and the change of microbial community during atrazine degradation were investigated. The results showed that bioaugmentation significantly accelerated the degradation rate of atrazine in soil and reduced the toxic effect of atrazine residues on wheat growth. The extra available NH4+ through atrazine mineralization could serve as a nitrogen source to increase microbial numbers. High-throughput sequencing further revealed that the microbial community restored a new balance. The function of microbial community predicted by PICRUSt2 suggested that the biodegradation process of atrazine affected not only the atrazine degradation pathway, but also the nitrogen metabolism pathway. Methylobacillus and Pseudomonas were considered as the most important indigenous atrazine-degrading microorganisms, because their relative abundances were positively correlated with the relative abundance of Paenarthrobacter and atrazine degradation pathway. This study provides insight into the cooperation between indigenous microorganisms and external inoculums on atrazine degradation process.


Assuntos
Atrazina , Herbicidas , Poluentes do Solo , Atrazina/análise , Atrazina/toxicidade , Biodegradação Ambiental , Herbicidas/análise , Herbicidas/toxicidade , Solo , Microbiologia do Solo , Poluentes do Solo/análise
20.
Biomater Sci ; 9(15): 5209-5217, 2021 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-34160487

RESUMO

Photothermal therapy, assisted by local heat generation using photothermal nanoparticles (NPs), is an emerging strategy to treat tumors noninvasively. To improve treatment outcomes and to alleviate potential side effects on normal tissue cells, utilizing the optically transparent second near-infrared (NIR-II) window and actively targeting tumors are critical. Considering that mitochondria are heat sensitive and play an important role in the up-regulation of metabolic activity in tumor cells, herein we report a cascade targeting scheme that enables active photothermal ablation of tumor mitochondria. First, NIR-II absorbing CuS NPs were surface modified with the mitochondria targeting moiety (3-carboxypropyl) triphenylphosphonium bromide (TPP) and then shielded with CD44 targeting hyaluronic acid, which will only expose TPP upon reaching the tumor sites. This allowed over 90% CuS NP enrichment at tumor mitochondria, and as a result, significantly improved tumor cell photothermal ablation was observed at the cellular level. An in vivo study demonstrated enhanced tumor uptake and improved tumor growth suppression by using these cascade targeting CuS NPs as NIR-II photothermal agents.


Assuntos
Nanopartículas , Fototerapia , Cobre , Mitocôndrias , Terapia Fototérmica
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