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1.
J Environ Manage ; 301: 113894, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34638045

RESUMO

A biotic iron (Fe0) treatment system combined with mixed microorganisms was applied to remediate cadmium (Cd)-contaminated groundwater under the intervention of sulfate. Due to hydrogenotrophic desulfuration effect, severe iron corrosion was observed in this microbe-collaborative Fe0 system according to surface morphology analysis as lots of secondary minerals (e.g. magnetite, green rust and lepidocrocite) were generated, which was essential for Cd(II) adsorption and immobilization. The sulfate-mediated biotic Fe0 system thereafter achieved a significantly enhanced Cd(II) removal efficiency of 86.1%, over 3.3 times than that in the abiotic Fe0 system. Increasing initial sulfate concentration could improve the removal of cadmium, which further proved that hydrogenotrophic desulfuration played a key role for enhanced Cd removal. According to the experimental results and current reports, the mechanism of Cd(II) removal was revealed into three pathways including adsorption to secondary iron minerals, co-precipitation with iron (hydr)oxides and formation of cadmium sulfide precipitation. Increasing Fe0 dosages showed positive correlation to Cd(II) removal and neutral pH was preferred to sulfate-mediated biotic Fe0 corrosion. These results indicated that sulfate-mediated biotic Fe0 corrosion could greatly relieve the limitation of Fe0 in Cd(II) immobilization, which could be a promising method to eliminate Cd(II) pollution from groundwater.


Assuntos
Cádmio , Água Subterrânea , Cádmio/análise , Corrosão , Ferro , Sulfatos
2.
Mater Horiz ; 8(1): 250-258, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34821303

RESUMO

Progress toward the development of wearable electromechanical sensors with durable and reliable sensing performance is critical for emerging wearable integrated electronic applications. However, it remains a long-standing challenge to realize mechanically stretchable sensing materials with extremely durable and high-performing sensing ability due to the fundamental dilemma lying in the sensing mechanism. In this work, we proposed an in situ and rapid self-healing strategy through nano-confining a dynamic host-guest supramolecular polymer network in a graphene-based multilevel nanocomposite matrix to fabricate a mechanically stretchable and structurally healable sensing nanocomposite which is provided with intriguing sensing durability and sensitivity simultaneously. When repeatedly stretching and releasing the nanocomposite sensing film, the fast association kinetics of cyclodextrin and adamantane host-guest inclusion complexes and good polymer chain dynamics in the supramolecular polymer network endowed by the nanoconfinement effect enable autonomous and rapid repair of the micro-cracks in situ generated in the sensing material. As a result, our strain sensing devices can achieve an extremely high durability and retain stable sensing performance even after over 100 000 stretching-releasing cycles at large strain of 50%. Moreover, the brittle nature originated from the inorganically dominated structure in conjunction with the thermodynamically stable host-guest interactions and dynamic hydrogen bonds inside the multilevel nanocomposite allow the sensing material to exhibit an ultrahigh gauge factor over 1500 with a large working strain of 58%. This work presents a reliable approach for the construction of ultradurable and high-performing wearable electronics.

3.
Nanomedicine (Lond) ; 16(24): 2207-2242, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34533048

RESUMO

With the excellent ability to transform near-infrared light to localized visible or UV light, thereby achieving deep tissue penetration, lanthanide ion-doped upconversion nanoparticles (UCNP) have emerged as one of the most striking nanoscale materials for more effective and safer cancer treatment. Up to now, UCNPs combined with photosensitive components have been widely used in the delivery of chemotherapy drugs, photodynamic therapy and photothermal therapy. Applications in these directions are reviewed in this article. We also highlight microenvironmental tumor monitoring and precise targeted therapies. Then we briefly summarize some new trends and the existing challenges for UCNPs. We hope this review can provide new ideas for future cancer treatment based on UCNPs.


Assuntos
Elementos da Série dos Lantanídeos , Nanopartículas , Neoplasias , Fotoquimioterapia , Humanos , Raios Infravermelhos , Elementos da Série dos Lantanídeos/uso terapêutico , Neoplasias/tratamento farmacológico
4.
Biomed Res Int ; 2021: 1078019, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34497845

RESUMO

Objective: To investigate the role of curcumin in regulating pathogenesis of pulmonary arterial smooth muscle cells (PASMCs) derived from pulmonary arterial hypertension (PAH) model. Methods: Male Sprague Dawley rats were injected with monocrotaline (MCT) to establish the PAH experimental model. The rats were divided into control group, MCT group, and curcumin group. At the end of the study, hemodynamic data were measured to determine pulmonary hypertension. Proliferation ability of PASMCs, a remodeling indicator of pulmonary artery and right ventricle, was detected. In addition, the morphology and function of mitochondria, antiglycolysis and antiproliferation pathways, and genes were also analyzed. Results: Curcumin may function by reversing MCT-mediated pulmonary vascular remodeling in rats. Curcumin effectively improved pulmonary vascular remodeling, promoted PASMC apoptosis, and protected mitochondrial function. In addition, curcumin treatment suppressed the PI3K/AKT pathway in PASMCs and regulated the expression of antiproliferative genes. Conclusion: Curcumin can improve energy metabolism and reverse the process of PAHS. However, there were side effects of curcumin in MCT-induced rats, suggesting that the dosage should be treated with caution and its toxicological mechanism should be further studied and evaluated.


Assuntos
Curcumina/farmacologia , Mitocôndrias/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Fosfatidilinositol 3-Quinase/metabolismo , Hipertensão Arterial Pulmonar/tratamento farmacológico , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Modelos Animais de Doenças , Hemodinâmica/efeitos dos fármacos , Masculino , Mitocôndrias/metabolismo , Miócitos de Músculo Liso/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Hipertensão Arterial Pulmonar/metabolismo , Hipertensão Arterial Pulmonar/patologia , Ratos , Transdução de Sinais/efeitos dos fármacos
5.
Int J Biol Sci ; 17(12): 2984-2999, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34421344

RESUMO

Background: Chronic diabetes accelerates vascular dysfunction often resulting in cardiomyopathy but underlying mechanisms remain unclear. Recent studies have shown that the deregulated unfolded protein response (UPR) dependent on highly conserved IRE1α-spliced X-box- binding protein (XBP1s) and the resulting endoplasmic reticulum stress (ER-Stress) plays a crucial role in the occurrence and development of diabetic cardiomyopathy (DCM). In the present study, we determined whether targeting MAPK/ERK pathway using MEK inhibitor U0126 could ameliorate DCM by regulating IRE1α-XBP1s pathway. Method: Three groups of 8-week-old C57/BL6J mice were studied: one group received saline injection as control (n=8) and two groups were made diabetic by streptozotocin (STZ) (n=10 each). 18 weeks after STZ injection and stable hyperglycemia, one group had saline treatment while the second group was treated with U0126 (1mg/kg/day), 8 weeks later, all groups were sacrificed. Cardiac function/histopathological changes were determined by echocardiogram examination, Millar catheter system, hematoxylin-eosin staining and western blot analysis. H9C2 cardiomyocytes were employed for in vitro studies. Results: Echocardiographic, hemodynamic and histological data showed overt myocardial hypertrophy and worsened cardiac function in diabetic mice. Chronic diabetic milieu enhanced SUMOylation and impaired nuclear translocation of XBP1s. Intriguingly, U0126 treatment significantly ameliorated progression of DCM, and this protective effect was achieved through enriching XBP1s' nuclear accumulation. Mechanistically, U0126 inhibited XBP1s' phosphorylation on S348 and SUMOylation on K276 promoting XBP1s' nuclear translocation. Collectively, these results identify that MEK inhibition restores XBP1s-dependent UPR and protects against diabetes-induced cardiac remodeling. Conclusion: The current study identifies previously unknown function of MEK/ERK pathway in regulation of ER-stress in DCM. U0126 could be a therapeutic target for the treatment of DCM.

6.
Nat Genet ; 53(9): 1385-1391, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34373642

RESUMO

Cotton produces natural fiber for the textile industry. The genetic effects of genomic structural variations underlying agronomic traits remain unclear. Here, we generate two high-quality genomes of Gossypium hirsutum cv. NDM8 and Gossypium barbadense acc. Pima90, and identify large-scale structural variations in the two species and 1,081 G. hirsutum accessions. The density of structural variations is higher in the D-subgenome than in the A-subgenome, indicating that the D-subgenome undergoes stronger selection during species formation and variety development. Many structural variations in genes and/or regulatory regions potentially influencing agronomic traits were discovered. Of 446 significantly associated structural variations, those for fiber quality and Verticillium wilt resistance are located mainly in the D-subgenome and those for yield mainly in the A-subgenome. Our research provides insight into the role of structural variations in genotype-to-phenotype relationships and their potential utility in crop improvement.


Assuntos
Fibra de Algodão/análise , Genoma de Planta/genética , Gossypium/genética , Gossypium/fisiologia , Agricultura/métodos , Ligação Genética , Variação Genética/genética , Genótipo , Gossypium/classificação , Fenótipo , Locos de Características Quantitativas/genética , Análise de Sequência de DNA , Indústria Têxtil/métodos
7.
Am J Transl Res ; 13(6): 6790-6795, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34306428

RESUMO

OBJECTIVE: To evaluate the therapeutic efficacy and serum inflammatory factors changes of influenza patients received Lianhua Qingwen granule combined with peramivir sodium chloride injection. METHODS: The clinical data of 100 influenza patients enrolled in our infirmary from January 2018 to January 2020 were retrospectively analyzed. Among them, 50 patients received eramivir sodium chloride injection (the control group) and 50 patients received Lianhua Qingwen granule plus (the experimental group). The clinical efficacy and serum inflammatory factor, including C-reactive protein (CRP), interleukin (IL)-6, and procalcitonin (PCT) were recorded and compared. RESULTS: The overall effective rate of the experimental group was significantly higher than that of the control group (96.0% vs. 80%, P=0.014). There was difference in the antipyretic time, sore throat relief time, cough relief time and general ache relief time between the two groups (all P < 0.05). Before treatment, there was no difference in IL-6, CRP, and PCT levels between the two groups (all P > 0.05). After treatment, the IL-6, CRP, and PCT levels were decreased in the two groups, and lower in the experimental group compared with the control group (all P=0.014). The ADRs rate in the experimental group was significantly lower than that of the control group (6.0% vs. 24%, χ2=6.353, P=0.012). CONCLUSION: Lianhua Qingwen granule combined with peramivir sodium chloride injection shows a remarkable potency in influenza patients. It can reduce the treatment span and improve the inflammatory factors, which is worthy of clinical promotion and application.

8.
Eur J Pediatr ; 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-34223969

RESUMO

To investigate the association of chronic hypertension, gestational hypertension, and preeclampsia diseases with infant growth in the first 36 months of life, we conducted a retrospective birth cohort of 31,734 children born in Zhoushan Maternal and Child Care Hospital between January 2001 and May 2018. Birthweight, gestational age, and infant growth (weight, height, weight/height-for-age Z score, the weight gain during childhood) were the main outcomes. The associations of chronic hypertension, gestational hypertension, and preeclampsia diseases with birth outcomes and infant growth at children's age of 3, 6, 12, 18, and 24 months were analyzed by multivariable regression models. Gestational hypertension, preeclampsia diseases, and chronic hypertension were significantly associated with lower birthweight and shorter gestational age. Both gestational hypertension and preeclampsia diseases were respectively inversely associated with weight, weight-for-age Z score, height, and height-for-age Z score of children in the whole sample and sub-sample data analysis from birth to the age of 36 months, although correction for birthweight rendered the associations nonsignificant. No significant association of gestational hypertension, preeclampsia diseases, and chronic hypertension with weight gain was found. Conclusion: The inverse associations of gestational hypertension and preeclampsia diseases with infant growth in early childhood were mainly mediated by the effect of gestational hypertension and preeclampsia diseases on lower birthweight. What is Known: • Hypertensive disorders of pregnancy are associated with increased risk of adverse birth outcomes. What is New: • Both gestational hypertension and preeclampsia were respectively inversely associated with physical development of offspring from birth to the age of 36 months. • Lower birthweight might be the mediator of the inverse associations of gestational hypertension and preeclampsia diseases with infant growth in early childhood.

9.
J Environ Qual ; 50(5): 1123-1134, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34213024

RESUMO

In this study, a collaborative system of Fe0 and mixed anaerobic microorganisms was established for remediating chromium (Cr)-contaminated soil and restraining the translocation of Cr from soil to swamp cabbage (Ipomoea aquatica Forssk.). Solid phase characterization demonstrated that more reactive secondary minerals such as green rust, magnetite, and lepidocrocite were generated in the composite system as compared with the Fe0 -only system. Hence, the Fe0 -microorganisms composite system achieved a remarkably higher aqueous Cr(VI) removal of 85.6%, 2.9 times higher than that in the Fe0 -only system. After 14 d remediation, easily available Cr(VI) and Crtotal species such as water-soluble, exchangeable, and bound-to-carbonates were converted to less available Cr(III) and Crtotal species (e.g., Fe-Mn oxides-bound and organic matter-bound species) because of the production of Cr-Fe hydroxides and oxides [Crx Fe1-x (OH)3 or Crx Fe1-x OOH] on the Fe0 surface. A pot experiment showed that Cr uptake by swamp cabbage after the composite system remediation was suppressed by 69.1%, two times higher than that after the Fe0 -only system remediation. Excessive Fe uptake by swamp cabbage also was efficiently inhibited by the composite system treatment due to enhanced Fe hydroxides and oxides production on the Fe0 surface because of biological corrosion and mineralization. These results indicated that Fe0 -microorganisms composite system remediation could efficiently enhance Cr(VI) immobilization and decrease its bioavailability and bioaccumulation by plants, which is a promising technology in Cr-contaminated soil remediation.


Assuntos
Benchmarking , Poluentes do Solo , Cromo/análise , Solo , Poluentes do Solo/análise
10.
Plant Biotechnol J ; 19(10): 2126-2138, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34160879

RESUMO

Verticillium wilt (VW) is a destructive disease that results in great losses in cotton yield and quality. Identifying genetic variation that enhances crop disease resistance is a primary objective in plant breeding. Here we reported a GWAS of cotton VW resistance in a natural-variation population, challenged by different pathogenicity stains and different environments, and found 382 SNPs significantly associated with VW resistance. The associated signal repeatedly peaked in chromosome Dt11 (68 798 494-69 212 808) containing 13 core elite alleles undescribed previously. The core SNPs can make the disease reaction type from susceptible to tolerant or resistant in accessions with alternate genotype compared to reference genotype. Of the genes associated with the Dt11 signal, 25 genes differentially expressed upon Verticillium dahliae stress, with 21 genes verified in VW resistance via gene knockdown and/or overexpression experiments. We firstly discovered that a gene cluster of L-type lectin-domain containing receptor kinase (GhLecRKs-V.9) played an important role in VW resistance. These results proved that the associated Dt11 region was a major genetic locus responsible for VW resistance. The frequency of the core elite alleles (FEA) in modern varieties was significantly higher than the early/middle varieties (12.55% vs 4.29%), indicating that the FEA increased during artificial selection breeding. The current developmental resistant cultivars, JND23 and JND24, had fixed these core elite alleles during breeding without yield penalty. These findings unprecedentedly provided genomic variations and promising alleles for promoting cotton VW resistance improvement.


Assuntos
Verticillium , Ascomicetos , Cromossomos , Resistência à Doença/genética , Genômica , Gossypium/genética , Melhoramento Vegetal , Doenças das Plantas/genética , Locos de Características Quantitativas
11.
Hum Cell ; 34(5): 1310-1323, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33977502

RESUMO

Preeclampsia (PE) is a serious complication of pregnancy. Exosomes are known to be upregulated in PE. In this study, we sought to investigate the effect of miR-486-5p from human placental microvascular endothelial cells, on the function of trophoblast cells. To investigate the function of human placental microvascular endothelial cell (HPVEC)-derived exosomes on trophoblast cells, HPVECs were treated with hypoxia/reoxygenation (H/R). The separation efficiency of exosomes was determined by transmission electron microscopy, nanosight and Western blot. Cell Counting Kit-8, EdU staining, wound-healing, and transwell assay were performed to detect the effect of exosomally transferred miR-486-5p inhibitor on proliferation, migration and invasion of trophoblast cells. MiRDB and dual-luciferase report assay were used to find the target of miR-486-5p. Our data revealed that miR-486-5p was significantly upregulated in H/R-treated HPVEC-Exo, and miR-486-5p was enriched in HPVEC-Exo. miR-486-5p inhibitor carried by HPVEC-Exo significantly inhibited the proliferation, migration and invasion of trophoblast cells. Insulin-like growth factor 1 (IGF1) was found to be the target of miR-486-5p, and IGF1 overexpression notably reversed the effect of miR-486-5p inhibitor from HPVEC-Exo on trophoblast cell function. In summary, H/R-treated HPVEC-derived exosomally expressing miR-486-5p inhibitor significantly inhibited the proliferation, migration and invasion of trophoblast cells via downregulation of IGF1. The findings from the present study may be useful in the development of treatments for PE.

12.
Plant J ; 107(3): 831-846, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34008265

RESUMO

Cotton (Gossypium hirsutum) is constantly attacked by pathogens and insects. The most efficient control strategy is to develop resistant varieties using broad-spectrum gene resources. Several resistance loci harboured by superior varieties have been identified through genome-wide association studies. However, the key genes and/or loci have not been functionally identified. In this study, we identified a locus significantly associated with Verticillium wilt (VW) resistance, and within a 145.5-kb linkage disequilibrium, two non-specific lipid transfer protein genes (named GhnsLTPsA10) were highly expressed under Verticillium pathogen stress. The expression of GhnsLTPsA10 significantly increased in roots upon Verticillium dahliae stress but significantly decreased in leaves under insect attack. Furthermore, GhnsLTPsA10 played antagonistic roles in positively regulating VW and Fusarium wilt resistance and negatively mediating aphid and bollworm resistance in transgenic Arabidopsis and silenced cotton. By combining transcriptomic, histological and physiological analyses, we determined that GhnsLTPsA10-mediated phenylpropanoid metabolism further affected the balance of the downstream metabolic flux of flavonoid and lignin biosynthesis. The divergent expression of GhnsLTPsA10 in roots and leaves coordinated resistance of cotton against fungal pathogens and insects via the redirection of metabolic flux. In addition, GhnsLTPsA10 contributed to reactive oxygen species accumulation. Therefore, in this study, we elucidated the novel function of GhnsLTP and the molecular association between disease resistance and insect resistance, balanced by GhnsLTPsA10. This broadens our knowledge of the biological function of GhnsLTPsA10 in crops and provides a useful locus for genetic improvement of cotton.

13.
J Colloid Interface Sci ; 599: 168-177, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33933791

RESUMO

Currently, engineering non-precious NiFe layered double hydroxide (NiFe-LDH) electrocatalysts with excellent oxygen evolution performances at high current densities is highly critical to promoting electrolytic water splitting producing hydrogen for large-scale commercial applications. Herein, an intrigued oxygen vacancy-rich Fe(Ⅱ)-incorporated NiFe-LDH containing electroactive high-valence ferritic species is successfully grown on Ni foam (Fe2+-NiFe-LDH-EO6 h@NF) through an elaborate two-step route including hydrothermal and electrooxidation, and utilized as a high-efficiency elctrocayalyst of alkaline water oxidation possessing abundant exposed active sites, excellent intrinsic catalytic activity and superior durability. Therefore, the Fe2+-NiFe-LDH-EO6 h@NF electrocatalyst towards oxygen evolution reaction (OER) enables the low overpotentials of 239, 285 and 350 mV for the current densities of 10, 100 and 500 mA cm-2, respectively, a small Tafel slope of 48.3 mV dec-1, the low onset potential of 1.451 V, and retains the catalytic activity for 40 h at the large current density of 500 mA cm-2 as well as owns the high turnover frequency (TOF) value of 0.93 s-1 at the overpotential of 300 mV. This work provides a promising avenue to improve the OER performances of NiFe-LDH electrocatalyst for practical applications.

14.
J Steroid Biochem Mol Biol ; 210: 105875, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33746111

RESUMO

XX sex reversal, also called XX disorders of sex development (XX-DSD), is a condition affecting the development of the gonads or genitalia, and is relatively common in pigs. However, its genetic etiology and transcriptional regulation mechanism in the hypothalamic-pituitary-gonadal axis (HPGA) remain mostly unknown. XX-DSD (SRY-negative) pigs and normal sows were selected by external genitalia observation. The hypothalamus, which is the integrated center of the HPGA was sampled for whole-transcriptome RNA-seq. The role of DEmiRNA was validated by its overexpression and knockdown in vitro. A total of 1,258 lncRNAs, 1,086 mRNAs, and 61 microRNAs differentially expressed in XX-DSD pigs compared with normal female pigs. Genes in the hormone biosynthesis and secretion pathway significantly up-regulated, and the up-regulation of GNRH1, KISS1 and AVP may associate with the abnormal secretion of GnRH. We also predicted the lncRNA-miRNA-mRNA co-expression triplets and constructed three competing endogenous RNA (ceRNA) potentially associated with XX-DSD. Functional enrichment studies suggested that TCONS_00340886, TCONS_00000204 and miR-181a related to GnRH secretion. Further, miR-181a inhibitor up-regulated GNRH1, PAK6, and CAMK4 in the GT1-7 cells. Conversely, transfection of miR-181a mimics obtained the opposite trends. The expression levels of FSHR, LHR, ESR1 and ESR2 were significantly higher in XX-DSD gondas than those in normal sows. Taken together, we proposed that the balance of endocrine had broken in XX-DSD pigs. The current study is the first to examine the transcriptomic profile in the hypothalamus of XX-DSD pigs. It provides new insight into coding and non-coding RNAs that may be associated with DSD in pigs.


Assuntos
Transtornos do Desenvolvimento Sexual/genética , Hipotálamo/fisiologia , MicroRNAs/genética , Transtornos Testiculares 46, XX do Desenvolvimento Sexual/genética , Transtornos Testiculares 46, XX do Desenvolvimento Sexual/veterinária , Animais , Transtornos do Desenvolvimento Sexual/veterinária , Feminino , Perfilação da Expressão Gênica , Mapas de Interação de Proteínas/genética , RNA Longo não Codificante/genética , RNA Mensageiro/genética , Receptores de Estrogênio/genética , Receptores do FSH/genética , Proteína da Região Y Determinante do Sexo/genética , Suínos , Doenças dos Suínos/genética
15.
Nat Commun ; 12(1): 1346, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33649323

RESUMO

SARS-CoV-2 is the underlying cause for the COVID-19 pandemic. Like most enveloped RNA viruses, SARS-CoV-2 uses a homotrimeric surface antigen to gain entry into host cells. Here we describe S-Trimer, a native-like trimeric subunit vaccine candidate for COVID-19 based on Trimer-Tag technology. Immunization of S-Trimer with either AS03 (oil-in-water emulsion) or CpG 1018 (TLR9 agonist) plus alum adjuvants induced high-level of neutralizing antibodies and Th1-biased cellular immune responses in animal models. Moreover, rhesus macaques immunized with adjuvanted S-Trimer were protected from SARS-CoV-2 challenge compared to vehicle controls, based on clinical observations and reduction of viral loads in lungs. Trimer-Tag may be an important platform technology for scalable production and rapid development of safe and effective subunit vaccines against current and future emerging RNA viruses.


Assuntos
Vacinas contra COVID-19/uso terapêutico , COVID-19/imunologia , COVID-19/prevenção & controle , SARS-CoV-2/patogenicidade , Animais , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Western Blotting , COVID-19/terapia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunidade Celular/fisiologia , Imunização Passiva , Imuno-Histoquímica , Macaca mulatta , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , SARS-CoV-2/imunologia
16.
Anim Biosci ; 34(11): 1757-1765, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33677912

RESUMO

OBJECTIVE: The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. METHODS: Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. RESULTS: In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. CONCLUSION: This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs.

17.
Patient Educ Couns ; 104(7): 1636-1643, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33526337

RESUMO

OBJECTIVE: To assess the effectiveness of empowerment programs on mental health in parents of preterm infants. METHODS: Ten databases were searched for randomised controlled trials and quasi-experimental studies published from inception to March 2020, and two reviewers independently and critically assessed trial quality and extracted data. RESULTS: Eight studies with a total of 1029 participants met the inclusion criteria. Empowerment programs significantly improved parental mental health, particularly maternal stress and depression. This meta-analysis also indicated that Creating Opportunities for Parent Empowerment (COPE) had a medium effect on maternal mental health. Both empowerment-based health education and empowerment programs conducted during the infants' hospitalisation had large effects on maternal mental health, whereas those conducted from hospitalisation to one week after discharge showed no significant effect. CONCLUSION: Empowerment programs have positive effects on parental mental health, especially for mothers. PRACTICE IMPLICATIONS: Our study provides objective evidence for the development of future empowerment programs.


Assuntos
Recém-Nascido Prematuro , Saúde Mental , Escolaridade , Feminino , Humanos , Lactente , Recém-Nascido , Mães , Pais
18.
BMC Plant Biol ; 21(1): 68, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33526028

RESUMO

BACKGROUND: Verticillium wilt is a widespread and destructive disease, which causes serious loss of cotton yield and quality. Long non-coding RNA (lncRNA) is involved in many biological processes, such as plant disease resistance response, through a variety of regulatory mechanisms, but their possible roles in cotton against Verticillium dahliae infection remain largely unclear. RESULTS: Here, we measured the transcriptome of resistant G. hirsutum following infection by V. dahliae and 4277 differentially expressed lncRNAs (delncRNAs) were identified. Localization and abundance analysis revealed that delncRNAs were biased distribution on chromosomes. We explored the dynamic characteristics of disease resistance related lncRNAs in chromosome distribution, induced expression profiles, biological function, and these lncRNAs were divided into three categories according to their induced expression profiles. For the delncRNAs, 687 cis-acting pairs and 14,600 trans-acting pairs of lncRNA-mRNA were identified, which indicated that trans-acting was the main way of Verticillium wilt resistance-associated lncRNAs regulating target mRNAs in cotton. Analyzing the regulation pattern of delncRNAs revealed that cis-acting and trans-acting lncRNAs had different ways to influence target genes. Gene Ontology (GO) enrichment analysis revealed that the regulatory function of delncRNAs participated significantly in stimulus response process, kinase activity and plasma membrane components. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that delncRNAs participated in some important disease resistance pathways, such as plant-pathogen interaction, alpha-linolenic acid metabolism and plant hormone signal transduction. Additionally, 21 delncRNAs and 10 target genes were identified as being involved in alpha-linolenic acid metabolism associated with the biosynthesis of jasmonic acid (JA). Subsequently, we found that GhlncLOX3 might regulate resistance to V. dahliae through modulating the expression of GhLOX3 implicated in JA biosynthesis. Further functional analysis showed that GhlncLOX3-silenced seedlings displayed a reduced resistance to V. dahliae, with down-regulated expression of GhLOX3 and decreased content of JA. CONCLUSION: This study shows the dynamic characteristics of delncRNAs in multiaspect, and suggests that GhlncLOX3-GhLOX3-JA network participates in response to V. dahliae invasion. Our results provide novel insights for genetic improvement of Verticillium wilt resistance in cotton using lncRNAs.


Assuntos
Gossypium/genética , Gossypium/microbiologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , RNA Longo não Codificante/metabolismo , Verticillium/fisiologia , Sequência de Bases , Cromossomos de Plantas/genética , Ciclopentanos/metabolismo , Resistência à Doença/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Oxilipinas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/microbiologia , RNA Longo não Codificante/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Fatores de Tempo
19.
BMC Plant Biol ; 21(1): 89, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33568051

RESUMO

BACKGROUND: Dirigent (DIR) proteins mediate regioselectivity and stereoselectivity during lignan biosynthesis and are also involved in lignin, gossypol and pterocarpan biosynthesis. This gene family plays a vital role in enhancing stress resistance and in secondary cell-wall development, but systematical understanding is lacking in cotton. RESULTS: In this study, 107 GbDIRs and 107 GhDIRs were identified in Gossypium barbadense and Gossypium hirsutum, respectively. Most of these genes have a classical gene structure without intron and encode proteins containing a signal peptide. Phylogenetic analysis showed that cotton DIR genes were classified into four distinct subfamilies (a, b/d, e, and f). Of these groups, DIR-a and DIR-e were evolutionarily conserved, and segmental and tandem duplications contributed equally to their formation. In contrast, DIR-b/d mainly expanded by recent tandem duplications, accompanying with a number of gene clusters. With the rapid evolution, DIR-b/d-III was a Gossypium-specific clade involved in atropselective synthesis of gossypol. RNA-seq data highlighted GhDIRs in response to Verticillium dahliae infection and suggested that DIR gene family could confer Verticillium wilt resistance. We also identified candidate DIR genes related to fiber development in G. barbadense and G. hirsutum and revealed their differential expression. To further determine the involvement of DIR genes in fiber development, we overexpressed a fiber length-related gene GbDIR78 in Arabidopsis and validated its function in trichomes and hypocotyls. CONCLUSIONS: These findings contribute novel insights towards the evolution of DIR gene family and provide valuable information for further understanding the roles of DIR genes in cotton fiber development as well as in stress responses.


Assuntos
Produtos Agrícolas/genética , Evolução Molecular , Genes de Plantas , Gossypium/genética , Proteínas de Plantas/genética , Tetraploidia , Regulação da Expressão Gênica de Plantas , Variação Genética , Genótipo , Filogenia
20.
Clin Nutr ; 40(5): 3650-3660, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33423808

RESUMO

BACKGROUND & AIMS: This study aims to explore the associations of vitamin D (VD) metabolic pathway gene with 25(OH)D level in pregnant women and the interactions of SNP with season and VD supplement. METHODS: A total of 2658 pregnant women were selected from Zhoushan Pregnant Women Cohort study. Gestational 25(OH)D level and single nucleotide polymorphism (SNP) of VD metabolic pathway gene were detected. Multilinear regression models were used to estimate associations of SNPs with gestational 25(OH)D levels. Stratified analyses were performed to test the interactions of SNP with season and VD supplements. RESULTS: The mutations of rs2298849 and rs7041 on the GC gene were respectively associated with higher 25(OH)D in the first and third trimester; the mutations of seven SNPs (rs1155563, rs16846876, rs17467825, rs2282679, rs2298850, rs3755967, and rs4588) on the GC gene were respectively associated with lower 25(OH)D both in the first and third trimester, and lower changes in 25(OH)D during late pregnancy. The mutations of above seven SNPs, except for rs1155563, were also respectively associated with lower 25(OH)D in the second trimester, but to a lesser extent; Besides, pregnant women with mutation on CYP24A1-rs2209314 had a higher increment in 25(OH)D than their counterparts in the second trimester. The increasing dose effect of Gc isoform on 25(OH)D was observed. The associations of GC and LRP2 genes with 25(OH)D modified by season and VD supplements. CONCLUSIONS: The polymorphisms of VD metabolic pathway gene were associated with gestational 25(OH)D, and the associations differ by seasons and VD supplements. Gc isoform exerted a profound influence on gestational 25(OH)D.


Assuntos
Suplementos Nutricionais , Gravidez , Proteína de Ligação a Vitamina D/genética , Vitamina D , Adulto , China , Estudos de Coortes , Feminino , Humanos , Polimorfismo de Nucleotídeo Único/genética , Gravidez/sangue , Gravidez/genética , Gravidez/estatística & dados numéricos , Complicações na Gravidez/epidemiologia , Complicações na Gravidez/genética , Estações do Ano , Vitamina D/sangue , Vitamina D/genética , Vitamina D/metabolismo , Deficiência de Vitamina D/epidemiologia , Deficiência de Vitamina D/genética
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