Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.852
Filtrar
1.
Front Microbiol ; 13: 820089, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35558126

RESUMO

In Streptococcus mutans, we find that the histidine kinase WalK possesses the longest C-terminal tail (CTT) among all 14 TCSs, and this tail plays a key role in the interaction of WalK with its response regulator WalR. We demonstrate that the intrinsically disordered CTT is characterized by a conserved tryptophan residue surrounded by acidic amino acids. Mutation in the tryptophan not only disrupts the stable interaction, but also impairs the efficient phosphotransferase and phosphatase activities of WalRK. In addition, the tryptophan is important for WalK to compete with DNA containing a WalR binding motif for the WalR interaction. We further show that the tryptophan is important for in vivo transcriptional regulation and bacterial biofilm formation by S. mutans. Moreover, Staphylococcus aureus WalK also has a characteristic CTT, albeit relatively shorter, with a conserved W-acidic motif, that is required for the WalRK interaction in vitro. Together, these data reveal that the W-acidic motif of WalK is indispensable for its interaction with WalR, thereby playing a key role in the WalRK-dependent signal transduction, transcriptional regulation and biofilm formation.

2.
Exp Biol Med (Maywood) ; : 15353702221090184, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35502556

RESUMO

Exosomes mediate inflammation and immune responses. The aim of the study was to examine the expression profiles of plasma exosomal microRNAs (miRNAs) and analyze their target gene functions in participants with chronic rhinosinusitis with nasal polyps (CRSwNP). We measured plasma exosomal miRNAs in five patients with CRSwNP and five controls. Transcripts per million (TPM) was used to assess miRNAs expression and the Benjamini-Hochberg procedure was employed for multiple comparisons correction. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene ontology (GO) analyses revealed biological annotation and functional prediction of target genes. Compared with controls, we found that 159 exosomal miRNAs were differentially expressed by miRNA sequencing in CRSwNP. The top three upregulated miRNAs were novel_miR_677, novel_miR_1037, and novel_miR_79, while the top three downregulated miRNAs were novel_miR_192, novel_miR_1022, and novel_miR_4. The target functions in the GO and KEGG analyses included axon guidance, extracellular matrix (ECM)-receptor interaction, protein digestion and absorption, the calcium, the Hippo, the Notch, the ErbB, the cAMP signaling pathway, and focal adhesion. This study describes the dissection of plasma exosomal miRNA profiling in CRSwNP. Our findings may provide a certain basis for further mechanism research and exploration of diagnostic values.

3.
Artigo em Inglês | MEDLINE | ID: mdl-35533248

RESUMO

Mesenchymal stem cell-derived conditioned medium (MSC-CM) improves cardiac function, which is partly attributed to released paracrine factors. Since such cardioprotection is moderate and transient, it's essential to optimize MSC-CM effective components to alleviate myocardial injury. To optimize MSC-CM, MSCs were treated with or without lipopolysaccharides (LPSs) for 48 h (serum-free), and the supernatant was collected. Then, LPS-CM (MSC stimulated by LPS) was further treated with LPS remover (LPS Re-CM) or was concentrated with a 10-kDa cutoff filter (10 kDa-CM). ELISA showed that all pretreatments increased levels of VEGF, HGF, and IGF except LPS remover; 10 kDa-CM was superior to other-CM. CCK-8 displayed that viability of injured H9c2 cells enhanced with the increase of MSC-CM concentration. We also found 10 kDa-CM significantly alleviated H9c2 hypoxia/reoxygenation (H/R) injury, as evidenced by increased Bcl-2/Bax ratio, decreased the levels of LDH and cTn. TEM, TUNEL, and H&E staining confirmed 10 kDa-CM inhibited H/R-induced H9c2 morphological changes. Proteomic analysis identified 41 differentially expressed proteins in 10 kDa-CM, among which anti-inflammation, pro-angiogenesis, and anti-apoptosis were related to cardiac protection. This study indicates that 10 kDa-CM protects H9c2 cardiomyocytes from H/R injury by preserving most of the protective factors, such as VEGF, HGF, and IGF, in MSC-CM.

4.
Gen Psychiatr ; 35(2): e100712, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572772

RESUMO

Background: Neural oscillations directly reflect the rhythmic changes of brain activities during the resting state or while performing specific tasks. Abnormal neural oscillations have been discovered in patients with schizophrenia. However, there is limited evidence available on abnormal spontaneous neural oscillations in clinical high risk for psychosis (CHR-P). The brain signals recorded by the magnetoencephalography (MEG) technique are not to be disrupted by the skull and scalp. Methods: In this study, we applied the MEG technique to record the resting-state neural activities in CHR-P. This was followed by a detailed MEG analysis method including three steps: (1) preprocessing, which was band-pass filtering based on the 0.5-60 Hz frequency range, removal of 50 Hz power frequency interference, and removal of electrocardiography (ECG) and electrooculography (EOG) artefacts by independent component analysis; (2) time-frequency analysis, a multitaper time-frequency transformation based on the Hanning window, and (3) source localisation, an exact low-resolution brain electromagnetic tomography. The method was verified by comparing a participant with CHR-P with a healthy control during the MEG recordings with an eyes-closed resting state. Results: Experimental results show that the neural oscillations in CHR-P were significantly abnormal in the theta frequency band (4-7 Hz) and the delta frequency band (1-3 Hz). Also, relevant brain regions were located in the left occipital lobe and left temporo-occipital junction for the theta band and in the right dorsolateral prefrontal lobe and near orbitofrontal gyrus for the delta band. Conclusions: Abnormal neural oscillations based on specific frequency bands and corresponding brain sources may become biomarkers for high-risk groups. Further work will validate these characteristics in CHR-P cohorts.

6.
J Immunol Methods ; 506: 113279, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35533747

RESUMO

The glycosylated receptor-binding domain (glycoRBD) of SARS-CoV-2 can induce protective neutralizing antibodies to function as a vaccine. However, it is unclear whether vaccines using non-glycosylated RBD (non-glycoRBD) can induce protective immunity. Here, we report the efficacy of a SARS-CoV-2 non-glycoRBD vaccine produced by prokaryotic system in mice. The recombinant non-glycoRBD protein was overexpressed in Escherichia coli in the form of inclusion bodies, and was obtained after renaturation and three-step purification. From HPLC analysis, the purity of the RBD was 99%. Additionally, angiotensin converting enzyme 2 (ACE2)-binding assays revealed that E.coli-derived non-glycoRBD had binding activity consistent with glycoRBD. The RBD was formulated with CpG ODN and Al(OH)3 adjuvants and the obtained RBD candidate vaccine elicited potent antibody responses and neutralized SARS-CoV-2 wild-type, Delta, and Omicron pseudoviruses. In summary, our data showed that a non-glycoRBD candidate vaccine produced by E.coli provided a robust immune response and had pseudovirus neutralizing activity, making it a solid candidate vaccine for protection against SARS-CoV-2.

7.
Brain Behav ; : e2594, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35557046

RESUMO

OBJECTIVE: To investigate the effects of brain-derived neurotrophic factor (BDNF) overexpression in the ventrolateral periaqueductal gray (vlPAG) on behavioral changes in epilepsy-migraine comorbid rats. METHOD: We used an adeno-associated virus (AAV)-mediated vector to supplement BDNF in the vlPAG area prior to the establishment of a pilocarpine-nitroglycerin (Pilo-NTG) combination-induced comorbid model of epilepsy and migraine. Seizure- and migraine-related behaviors were analyzed. Cell loss and apoptosis in vlPAG were detected through hematoxylin-eosin (HE) and TUNEL staining. Immunofluorescence staining analyses were employed to detect expressions of BDNF and its receptor, tyrosine kinase B (TrkB), in vlPAG. Immunohistochemical staining was conducted to detect expressions of c-Fos and calcitonin gene-related peptide (CGRP) in the trigeminal nucleus caudalis (TNC) and trigeminal ganglion (TG). RESULTS: Comparing to control group, AAV-BDNF injected comorbid group showed lower pain sensitivity, scratching head, and spontaneous seizures accompanied by the downregulation of c-Fos labeling neurons and CGRP immunoreactivity in the TNC and TG. However, these changes were still significantly higher in the comorbid group than those in both epilepsy and migraine groups under the same intervention. CONCLUSION: These data demonstrated that supplying BDNF to vlPAG may protect structural and functional abnormalities in vlPAG and provide an antiepileptic and analgesic therapy.

8.
BMC Plant Biol ; 22(1): 235, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35534832

RESUMO

BACKGROUND: Sheath blight is an important disease caused by Rhizoctonia cerealis that affects wheat yields worldwide. No wheat varieties have been identified with high resistance or immunity to sheath blight. Understanding the sheath blight resistance mechanism is essential for controlling this disease. In this study, we investigated the response of wheat to Rhizoctonia cerealis infection by analyzing the cytological changes and transcriptomes of common wheat 7182 with moderate sensitivity to sheath blight and H83 with moderate resistance. RESULTS: The cytological observation showed that the growth of Rhizoctonia cerealis on the surface and its expansion inside the leaf sheath tissue were more rapid in the susceptible material. According to the transcriptome sequencing results, a total of 88685 genes were identified in both materials, including 20156 differentially expressed genes (DEGs) of which 12087 was upregulated genes and 8069 was downregulated genes. At 36 h post-inoculation, compared with the uninfected control, 11498 DEGs were identified in resistant materials, with 5064 downregulated genes and 6434 upregulated genes, and 13058 genes were detected in susceptible materials, with 6759 downregulated genes and 6299 upregulated genes. At 72 h post-inoculation, compared with the uninfected control, 6578 DEGs were detected in resistant materials, with 2991 downregulated genes and 3587 upregulated genes, and 7324 genes were detected in susceptible materials, with 4119 downregulated genes and 3205 upregulated genes. Functional annotation and enrichment analysis showed that the main pathways enriched for the DEGs included biosynthesis of secondary metabolites, carbon metabolism, plant hormone signal transduction, and plant-pathogen interaction. In particular, phenylpropane biosynthesis pathway is specifically activated in resistant variety H83 after infection. Many DEGs also belonged to the MYB, AP2, NAC, and WRKY transcription factor families. CONCLUSIONS: Thus, we suggest that the normal functioning of plant signaling pathways and differences in the expression of key genes and transcription factors in some important metabolic pathways may be important for defending wheat against sheath blight. These findings may facilitate further exploration of the sheath blight resistance mechanism in wheat and the cloning of related genes.


Assuntos
Transcriptoma , Triticum , Basidiomycota , Resistência à Doença/genética , Suscetibilidade a Doenças , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Doenças das Plantas/genética , Proteínas de Plantas/genética , Rhizoctonia/fisiologia , Fatores de Transcrição/genética , Triticum/metabolismo
9.
J Environ Manage ; 315: 115147, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35490485

RESUMO

Large quantities of landfill sludge (LS) with higher water content (WC) were stored underground, and excavation and re-dewatering of LS is a sustainable and economic strategy to save landfill space and reduce the leaching of contaminants. In this study, polyferric silicate sulfate (PFSS) was first applied in the conditioning of excavated LS, and the effects of the Si/Fe mass ratio and PFSS dosage on physicochemical properties, dewaterability and rheological properties were investigated. At the best Si/Fe of 0.18, PFSS conditioning obtained compact aggregates with the strongest internal structure, thus achieving the lowest WC. Large sludge flocs were formed, and slime and loosely-bound extracellular polymeric substances were effectively removed with the PFSS dosage above 100 mg/g dried solids, which made the WC to be lower than 51.4%. The whole mechanical compression process of conditioned LS can be described by the modified Terzaghi-Voigt model, and increasing the PFSS dosage induced the release of bound water and migration of the consolidation stage from ternary to secondary. PFSS is an economically sustainable conditioner for LS, integrating multiple functions such as charge neutralization, particle aggregation, interparticle bridging and skeleton building in one chemical.


Assuntos
Esgotos , Instalações de Eliminação de Resíduos , Esgotos/química , Silicatos , Sulfatos , Eliminação de Resíduos Líquidos , Água/química
10.
J Hazard Mater ; 435: 129021, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35490630

RESUMO

Dissolved organic matter released from biochar (biochar-derived DOM, BDOM) could dominate the environmental behavior and fate of trace metals by forming BDOM-metal complexes. Here general, heterospectral as well as moving-window (MW) two-dimensional correlation spectroscopy (2DCOS) analyses of synchronous fluorescence and Fourier transform infrared spectra were employed to explore the heterogeneous binding characteristics between sludge BDOM and Cu(II). The results revealed that Cu-BDOM binding first occurred in the fulvic-like (368-380 nm), then humic-like (428 nm) fluorescent fractions, followed by infrared groups of phenolic hydroxyl groups, carboxylate, COH of polysaccharide groups, CC of aromatic carbon, CH of aliphatics and COC of aliphatic ethers. The binding affinity of the hydrophilic groups was stronger than that of hydrophobic groups in BDOM towards Cu(II). Fluorescence components in BDOM played a decisive role in the binding of Cu(II) with trace concentration (1 µM), while infrared functional groups made a substantial contribution in the complexation of Cu(II) with higher concentration (10-100 µM). The concentration of final configuration transformation point (11.7 µmol/mg in this study) by MW2DCOS analysis was suggested as an actual binding threshold that was helpful for evaluating their environmental behaviors.

11.
Adv Drug Deliv Rev ; : 114320, 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35526664

RESUMO

As a non-invasive imaging monitoring method, molecular imaging can provide the location and expression level of disease signature biomolecules in vivo, leading to early diagnosis of relevant diseases, improved treatment strategies, and accurate assessment of treating efficacy. In recent years, a variety of nanosized imaging probes have been developed and intensively investigated in fundamental/translational research and clinical practice. Meanwhile, as an interdisciplinary discipline, this field combines many subjects of chemistry, medicine, biology, radiology, and material science, etc. The successful molecular imaging not only requires advanced imaging equipment, but also the synthesis of efficient imaging probes. However, limited summary has been reported for recent advances of nanoprobes. In this paper, we summarized the recent progress of three common and main types of nanosized molecular imaging probes, including ultrasound (US) imaging nanoprobes, magnetic resonance imaging (MRI) nanoprobes, and computed tomography (CT) imaging nanoprobes. The applications of molecular imaging nanoprobes were discussed in details. Finally, we provided an outlook on the development of next generation molecular imaging nanoprobes.

12.
Int J Mol Sci ; 23(9)2022 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-35563392

RESUMO

Plasmids are mostly found in bacteria as extrachromosomal genetic elements and are widely used in genetic engineering. Exploring the mechanisms of plasmid-host interaction can provide crucial information for the application of plasmids in genetic engineering. However, many studies have generally focused on the influence of plasmids on their bacterial hosts, and the effects of plasmids on bacteria-feeding animals have not been explored in detail. Here, we use a "plasmid-bacteria-Caenorhabditis elegans" model to explore the impact of plasmids on their host bacteria and bacterivorous nematodes. First, the phenotypic responses of C. elegans were observed by feeding Escherichia coli OP50 harboring different types of plasmids. We found that E. coli OP50 harboring plasmid pEX18Gm unexpectedly increases the fecundity of C. elegans. Subsequently, we found that the plasmid pEX18Gm indirectly affects C. elegans fecundity via bacterial metabolism. To explore the underlying regulatory mechanism, we performed bacterial RNA sequencing and performed in-depth analysis. We demonstrated that the plasmid pEX18Gm upregulates the transcription of methionine synthase gene metH in the bacteria, which results in an increase in methionine that supports C. elegans fecundity. Additionally, we found that a pEX18Gm-induced increase in C. elegans can occur in different bacterial species. Our findings highlight the plasmid-bacteria-C. elegans model to reveal the mechanism of plasmids' effects on their host and provide a new pattern for systematically studying the interaction between plasmids and multi-species.

13.
Foods ; 11(9)2022 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-35563948

RESUMO

In this study, nonvolatile metabolomics and proteomics were applied to investigate the change mechanism of flavonoid glycoside compounds during withering processing of white tea. With the extension of withering time, the content of the main flavonoid glycoside compounds significantly decreased, and then the flavonoid aglycones and water-soluble saccharides contents increased. However, the change trends of these compounds were inconsistent with the expression pattern of related biosynthesis pathway proteins, indicating that the degradation of flavonoid glycosides might exist in the withering process of white tea. One co-expression network that was highly correlated with variations in the flavonoid glycosides' component contents during the withering process was identified via WGCNA. Further analysis revealed that the degradation of flavonoid glycosides may be related to the antioxidant action of tea leaves undergoing the withering process. Our results provide a novel characterization of white tea taste formation during processing.

14.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 38(5): 419-424, 2022 May.
Artigo em Chinês | MEDLINE | ID: mdl-35603650

RESUMO

Objective To investigate whether tripartite motif containing 59 (TRIM59) influences the biological behavior of nasopharyngeal carcinoma cells by regulating the nuclear factor κB(NF-κB) signaling pathway. Methods TCGA database was used to predict the expression of TRIM59 in nasopharyngeal carcinoma and adjacent tissues. Reverse transcription PCR and Western blot were used to detect the relative expressions of TRIM59 mRNA and protein in nasopharyngeal carcinoma and paracancerous tissues. With human normal nasal mucosal epithelial cells (HNEpCs) as a control, reverse transcription PCR and Western blot analysis were used to detect the relative expression of TRIM59 mRNA and protein in HNE1 and CNE-2Z nasopharyngeal carcinoma cells. Small interference RNA technology was used to down-regulate the level of TRIM59 in HNE1 cells, while a control group, small interference RNA negative control (si-NC) group and TRIM59 small interference RNA (si-TRIM59) group were set up. MTT assay was used to detect the cell proliferation inhibition rate of each transfection group. TranswellTM chamber was used to detect cell invasion and migration ability of each transfection group. Western blot analysis was employed to detect NF-κB and phosphorylated NF-κB (p-NF-κB), vimentin, survivin protein expression. ELISA was adopted to detect interleukin 6 (IL-6), IL-8, tumor necrosis factor α (TNF-α) in the supernatant of cultured cells. Results The expression of TRIM59 increased abnormally in nasopharyngeal carcinoma tissues. Compared with HNEpC cells, the relative expression of TRIM59 in HNE1 significantly increased. After down-regulating the expression of TRIM59 in HNE1 cells, cell survival and cell invasion and migration were significantly inhibited. Down-regulating the expression of TRIM59 inhibited HNE1 cells p-NF-κB, vimentin, survivin protein expression and significantly reduced the levels of IL-6, IL-8, and TNF-α. Conclusion Down-regulation of TRIM59 blocks the NF-κB signaling pathway and tumor inflammation-related factors in HNE1 cells, thereby inhibiting cell invasion and migration.


Assuntos
NF-kappa B , Neoplasias Nasofaríngeas , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação para Baixo , Humanos , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular , NF-kappa B/metabolismo , Carcinoma Nasofaríngeo/genética , Neoplasias Nasofaríngeas/genética , Neoplasias Nasofaríngeas/patologia , RNA , RNA Mensageiro/genética , Transdução de Sinais/genética , Survivina/metabolismo , Proteínas com Motivo Tripartido , Fator de Necrose Tumoral alfa/metabolismo , Vimentina/metabolismo
15.
Angew Chem Int Ed Engl ; : e202204967, 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35510692

RESUMO

Cu-based metal-organic frameworks have attracted much attention for electrocatalytic CO2 reduction, but they are generally instable and difficult to control the product selectivity. We report flexible Cu(I) triazolate frameworks as efficient, stable, and tunable electrocatalysts for CO2 reduction to C2 H4 /CH4 . By changing the size of ligand side groups, the C2 H4 /CH4 selectivity ratio can be gradually tuned and inversed from 11.8 : 1 to 1 : 2.6, giving C2 H4 , CH4 , and hydrocarbon selectivities up to 51 %, 56 %, and 77 %, respectively. After long-term electrocatalysis, they can retain the structures/morphologies without formation of Cu-based inorganic species. Computational simulations showed that the coordination geometry of Cu(I) changed from triangular to tetrahedral to bind the reaction intermediates, and two adjacent Cu(I) cooperated for C-C coupling to form C2 H4 . Importantly, the ligand side groups controlled the catalyst flexibility by the steric hindrance mechanism, and the C2 H4 pathway is more sensitive than the CH4 one.

16.
EBioMedicine ; 80: 104022, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35512608

RESUMO

BACKGROUND: We aimed to develop a deep learning-based segmentation system for rapid on-site cytopathology evaluation (ROSE) to improve the diagnostic efficiency of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) biopsy. METHODS: A retrospective, multicenter, diagnostic study was conducted using 5345 cytopathological slide images from 194 patients who underwent EUS-FNA. These patients were from Nanjing Drum Tower Hospital (109 patients), Wuxi People's Hospital (30 patients), Wuxi Second People's Hospital (25 patients), and The Second Affiliated Hospital of Soochow University (30 patients). A deep convolutional neural network (DCNN) system was developed to segment cell clusters and identify cancer cell clusters with cytopathological slide images. Internal testing, external testing, subgroup analysis, and human-machine competition were used to evaluate the performance of the system. FINDINGS: The DCNN system segmented stained cells from the background in cytopathological slides with an F1-score of 0·929 and 0·899-0·938 in internal and external testing, respectively. For cancer identification, the DCNN system identified images containing cancer clusters with AUCs of 0·958 and 0·948-0·976 in internal and external testing, respectively. The generalizable and robust performance of the DCNN system was validated in sensitivity analysis (AUC > 0·900) and was superior to that of trained endoscopists and comparable to cytopathologists on our testing datasets. INTERPRETATION: The DCNN system is feasible and robust for identifying sample adequacy and pancreatic cancer cell clusters. Prospective studies are warranted to evaluate the clinical significance of the system. FUNDING: Jiangsu Natural Science Foundation; Nanjing Medical Science and Technology Development Funding; National Natural Science Foundation of China.

17.
Chemosphere ; 302: 134835, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35525459

RESUMO

Antibiotic pollution has caused important concern for international and national sustainability. Catalytic ozonation is a quick and efficient technique to remove contaminants in aquatic environment. This study firstly developed a nanosheet-growth technique for synthesizing Li-doped Mg(OH)2 with dot-sheet hierarchical structure as catalyst to ozonize antibiotics. Metronidazole could be totally removed through ozonation catalyzed by Li-doped Mg(OH)2 in 10 min. Approximately 97% of metronidazole was eliminated in 10 min even the catalyst was used for 4 times. Reaction rate constant of Li-doped Mg(OH)2 treatment was about 3.45 times that of nano-Mg(OH)2 treatment, illustrating that the dot-sheet hierarchical structure of Li-doped Mg(OH)2 exhibited nano-confinement effect on the catalytic ozonation. Approximately 70.4% of metronidazole was mineralized by catalytic ozonation using Li-doped Mg(OH)2. Temperature of 25 °C was more suitable for catalytic ozonation of metronidazole by Li-doped Mg(OH)2. Ions generally inhibited the catalytic ozonation of metronidazole while only 0.005 mol L-1 of Cl- slightly enhanced the ozonation rate, illustrating complicated mechanisms existed for ozonation of metronidazole catalyzed by Li-doped Mg(OH)2. The possible mechanisms of the ozonation of metronidazole using Li-doped Mg(OH)2 included direct ozonation and ozonation catalyzed by radical ·O2-, reactive oxygen species 1O2 and intermediate (H2O2). The synthesized Mg(OH)2 nanosheet with dot-sheet hierarchical structure is a novel nanoconfined material with excellent reusability and catalytic performance.

18.
Adv Sci (Weinh) ; 9(14): e2104333, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35403837

RESUMO

Coronavirus disease 2019 (COVID-19) remains a global public health threat. Hence, more effective and specific antivirals are urgently needed. Here, COVID-19 hyperimmune globulin (COVID-HIG), a passive immunotherapy, is prepared from the plasma of healthy donors vaccinated with BBIBP-CorV (Sinopharm COVID-19 vaccine). COVID-HIG shows high-affinity binding to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein, the receptor-binding domain (RBD), the N-terminal domain of the S protein, and the nucleocapsid protein; and blocks RBD binding to human angiotensin-converting enzyme 2 (hACE2). Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro. However, a significant reduction in the neutralization titer is detected against Beta, Delta, and Omicron variants. Additionally, assessments of the prophylactic and treatment efficacy of COVID-HIG in an Adv5-hACE2-transduced IFNAR-/- mouse model of SARS-CoV-2 infection show significantly reduced weight loss, lung viral loads, and lung pathological injury. Moreover, COVID-HIG exhibits neutralization potency similar to that of anti-SARS-CoV-2 hyperimmune globulin from pooled convalescent plasma. Overall, the results demonstrate the potential of COVID-HIG against SARS-CoV-2 infection and provide reference for subsequent clinical trials.

19.
Artigo em Inglês | MEDLINE | ID: mdl-35476394

RESUMO

Terahertz wave manipulations, especially the phase manipulations, through metasurfaces has attracted considerable interests. Here, we develop a terahertz beam steering device using the liquid-crystal (LC)-integrated programmable metasurface. Specifically, a reflective-type 1 bit metasurface element is designed by employing a multilayer structure composed of metallic back plate-LC-complementary split ring resonator (CSRR). Numerical simulations show that, at the optimized operation frequency of 0.675 THz, the developed metasurface element has a nearly 180° phase difference between unbiased and biased states with close reflection amplitudes. Furthermore, a one-dimensional programmable metasurface array with 32 independently controlled subarrays is designed and fabricated using the lithography technology. Both simulated and measured far-field scattering patterns of the metasurface certify the anomalous beam reflection and wide-angle beam steering performances. Nevertheless, the optimal frequency red shifts to 0.645 THz in the experiment. This work may advance the application of metasurfaces in terahertz beam manipulation devices.

20.
Zootaxa ; 5099(2): 293-300, 2022 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-35391412

RESUMO

The third species of Pseudonagoda Holloway, 1990, P. zhejiangensis sp. nov., is described from Zhejiang Province, China, and this little-known genus is reported for the first time from China. The new species is compared with its congeners P. siniaevi Solovyev, 2009 in morphological features. The male adult and genitalia are illustrated, as well as the COI sequences data are provided. The immature stages of the genus are also described firstly in this study.


Assuntos
Lepidópteros , Mariposas , Distribuição Animal , Animais , China , Genitália , Masculino
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...