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1.
Macromol Rapid Commun ; : e2100457, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34647394

RESUMO

In the past two decades, ionic conductive hydrogel has attracted tremendous research interests for their intrinsic characteristics in the field of flexible sensor. However, synchronous achievement of high mechanical strength, satisfied ionic conductivity, and broad adhesion to various substrates is still a challenge. Herein, a novel zwitterionic composite hydrogel that displayed excited strechability (up to 900%), satisfied strength (about 30 kPa), high ionic conductivity (1.2 mS cm-1 ), and adhesion to polar and nonpolar materials is fabricated though the combination of waterborne polyurethanes (PU) and poly(sulfobetaine zwitterion-co-acrylamide) (SAm). Especially, this facile strategy demonstrates that PU has a synergistic effect on enhancing mechanical strength and ionic conductivity for ionic conductive hydrogel. Moreover, the hydrogel-based strain/stress sensor shows high sensitivity, wide sensing range, great stability, and accuracy for human body movements detecting and voice recognition. This novel ionic conductive hydrogel has promoted the development of wearable devices.

2.
BMC Health Serv Res ; 21(1): 936, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34496839

RESUMO

BACKGROUND: This study aimed to reduce the total waiting time for high-end health screening processes. METHOD: The subjects of this study were recruited from a health screening center in a tertiary hospital in northern Taiwan from September 2016 to February 2017, where a total of 2342 high-end customers participated. Three policies were adopted for the simulation. RESULTS: The first policy presented a predetermined proportion of customer types, in which the total waiting time was increased from 72.29 to 83.04 mins. The second policy was based on increased bottleneck resources, which provided significant improvement, decreasing the total waiting time from 72.29 to 28.39 mins. However, this policy also dramatically increased the cost while lowering the utilization of this health screening center. The third policy was adjusting customer arrival times, which significantly reduced the waiting time-with the total waiting time reduced from 72.29 to 55.02 mins. Although the waiting time of this policy was slightly longer than that of the second policy, the additional cost was much lower. CONCLUSIONS: Scheduled arrival intervals could help reduce customer waiting time in the health screening department based on the "first in, first out" rule. The simulation model of this study could be utilized, and the parameters could be modified to comply with different health screening centers to improve processes and service quality.


Assuntos
Inteligência Ambiental , Análise de Dados , Simulação por Computador , Atenção à Saúde , Humanos , Projetos Piloto , Listas de Espera
3.
Artigo em Inglês | MEDLINE | ID: mdl-34511352

RESUMO

OBJECTIVE: Plate exposure remains one of the most serious complications of oromandibular reconstruction. The purpose of this study was to investigate the effect of wrapping reconstruction plates with anterolateral thigh (ALT) free flaps as a way to prevent plate exposure. STUDY DESIGN: A total of 91 patients with composite oromandibular defects who underwent surgical reconstruction using a reconstruction plate in conjunction with an ALT musculocutaneous flap were recruited. The participants were divided into 2 groups, based on whether the plate was wrapped with or covered by the ALT flap. RESULTS: The incidence of plate exposure in the experimental group (17%) was significantly lower than that of the control group (43.1%; P < .0001). The mean time from reconstruction until plate exposure in the experimental group was 19.4 ± 23.6 months, whereas the mean time in the control group was 12.3 ± 9.1 months. In the logistic regression model for the occurrence of plate exposure, the only factor found to be associated with plate exposure was how the ALT flap was used (P = .026). CONCLUSIONS: The results of the present study indicate that wrapping the reconstruction plate with an ALT flap decreased the incidence of plate exposure in patients with oromandibular defects.

4.
Front Endocrinol (Lausanne) ; 12: 731273, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34489872

RESUMO

Vascular aging is characterized by alterations in the constitutive properties and biological functions of the blood vessel wall. Endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) are indispensability elements in the inner layer and the medial layer of the blood vessel wall, respectively. Dipeptidyl peptidase-4 (DPP4) inhibitors, as a hypoglycemic agent, play a protective role in reversing vascular aging regardless of their effects in meliorating glycemic control in humans and animal models of type 2 diabetes mellitus (T2DM) through complex cellular mechanisms, including improving EC dysfunction, promoting EC proliferation and migration, alleviating EC senescence, obstructing EC apoptosis, suppressing the proliferation and migration of VSMCs, increasing circulating endothelial progenitor cell (EPC) levels, and preventing the infiltration of mononuclear macrophages. All of these showed that DPP4 inhibitors may exert a positive effect against vascular aging, thereby preventing vascular aging-related diseases. In the current review, we will summarize the cellular mechanism of DPP4 inhibitors regulating vascular aging; moreover, we also intend to compile the roles and the promising therapeutic application of DPP4 inhibitors in vascular aging-related diseases.

7.
J Int Med Res ; 49(8): 3000605211034945, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34344219

RESUMO

OBJECTIVE: To compare hepatitis C virus (HCV) RNA levels from serum and explanted native liver samples from patients undergoing living donor liver transplantation (LDLT). METHODS: This was a prospective observational study. Serum and liver samples were collected from consecutive serum anti-HCV-positive transplant recipients between February 2016 to August 2019. HCV RNA was extracted from liver samples and subjected to one-step reverse-transcription qPCR. using the TopScript One Step qRT-PCR Probe Kit with HCV qPCR probe assay and human GAPDH qPCR probe assay on a ViiA7 Real-Time PCR System. RESULTS: Among the 80 patients, 36% (29/80) were HCV RNA positive in serum and 85% (68/80) had positive hepatic HCV RNA. Post-liver transplantation, 4% (3/80) patients were serum positive. CONCLUSIONS: Our study suggests that pre-transplant serum HCV RNA levels may give an underestimate of the number of positive HCV RNA cases and that hepatic HCV RNA data may be more accurate.


Assuntos
Hepatite C , Transplante de Fígado , Hepacivirus/genética , Humanos , Fígado , Doadores Vivos , RNA , RNA Viral/genética
8.
Sensors (Basel) ; 21(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34450961

RESUMO

IoT technologies enable millions of devices to transmit their sensor data to the external world. The device-object pairing problem arises when a group of Internet of Things is concurrently tracked by cameras and sensors. While cameras view these things as visual "objects", these things which are equipped with "sensing devices" also continuously report their status. The challenge is that when visualizing these things on videos, their status needs to be placed properly on the screen. This requires correctly pairing visual objects with their sensing devices. There are many real-life examples. Recognizing a vehicle in videos does not imply that we can read its pedometer and fuel meter inside. Recognizing a pet on screen does not mean that we can correctly read its necklace data. In more critical ICU environments, visualizing all patients and showing their physiological signals on screen would greatly relieve nurses' burdens. The barrier behind this is that the camera may see an object but not be able to see its carried device, not to mention its sensor readings. This paper addresses the device-object pairing problem and presents a multi-camera, multi-IoT device system that enables visualizing a group of people together with their wearable devices' data and demonstrating the ability to recover the missing bounding box.


Assuntos
Dispositivos Eletrônicos Vestíveis , Atenção à Saúde , Humanos , Tecnologia
9.
Radiat Prot Dosimetry ; 195(2): 69-74, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34341830

RESUMO

A radioluminescence (RL) study of dose characteristics of tissue-equivalent LiF:Mg,Ti was carried out to determine the possible application as a real-time dosemeter. An RL measurements system based on LiF:Mg,Ti coupled with optical fiber was developed, and a blank fiber was set to remove the stem effect generated by the optical fiber due to direct radiation. A slight increase of RL sensitivity with accumulated dose and the afterglow effect due to shallow traps in LiF:Mg,Ti were observed, thus a set of algorithms was adopted to correct measured dose rate. A good linearity of dose-rate response using RL in LiF:Mg,Ti over more than four orders of magnitude (from 0.76 mGy/h to 8.02 Gy/h) was shown, and the deviation of calibrated dose rate is within 20%. Moreover, a satisfactory reproducibility (1.45%) of the measured dose rate after correction was represented. The results indicated that LiF:Mg,Ti might be promising for real-time dose monitoring.


Assuntos
Dosimetria Termoluminescente , Titânio , Algoritmos , Fluoretos , Compostos de Lítio , Doses de Radiação , Dosímetros de Radiação , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
10.
BMC Oral Health ; 21(1): 387, 2021 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-34362348

RESUMO

BACKGROUND: Salivary fistula is a relatively common complication in patients who have undergone a parotidectomy. The purpose of this study was to investigate the effects of bipolar coagulation forceps use on salivary fistulas. METHODS: From March 2015 to June 2020, 177 patients who underwent a parotidectomy in the Department of Oral and Maxillofacial Surgery at the Second Xiangya Hospital of Central South University were recruited. The patients were divided into an experimental group and a control group based on whether bipolar coagulation forceps or sutures were used, respectively. RESULTS: The drainage output of the experimental group was significantly lower than that of the control group (p = 0.04). The duration of dressing pressure applied in the experimental group was significantly shorter than that in the control group (p = 0.0003). Moreover, the incidence of salivary fistula in the experimental group (9.8%, 8/82) was notably lower than that in the control group (34.7%, 33/95) (p < 0.0001). In the logistic regression model for salivary fistula development, both the use of bipolar coagulation forceps (p = 0.0021) and drainage output (p = 0.0237) were associated with the presence of salivary fistulas. CONCLUSIONS: Our findings indicate that the use of bipolar coagulation forceps decreases the incidence of salivary fistula in patients who have undergone a parotidectomy. The use of bipolar coagulation forceps is a safe, effective, and convenient method to prevent salivary fistulas in patients who undergo a parotidectomy. TRIAL REGISTRATION: Current Controlled Trials ChiCTR2100044722, Date: 26/03/2021, Retrospectively registered.


Assuntos
Fístula , Complicações Pós-Operatórias , Drenagem , Humanos , Glândula Parótida/cirurgia , Complicações Pós-Operatórias/prevenção & controle , Estudos Retrospectivos , Instrumentos Cirúrgicos
11.
Cell Oncol (Dordr) ; 44(5): 1167-1181, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34460078

RESUMO

PURPOSE: Chemotherapy based on cisplatin (CDDP) has been established as the treatment of choice for head and neck squamous cell carcinoma (HNSCC). Malignant tumors respond to microenvironmental alterations through a dynamic balance between mitochondrial fission and fusion. HNSCCs are known to exhibit hypoxic conditions, yet the respective effects and underlying mechanisms of hypoxia on chemosensitivity and mitochondrial dynamics remain to be resolved. METHODS: The effect of hypoxia on the chemosensitivity of HNCC cells was determined by flow cytometry. Mitochondrial fission factor (Mff) expression was assessed by RT-PCR and Western blotting in hypoxic HNSCC cells, and further verified in primary CDDP-sensitive and CDDP-resistant HSNCC samples. The biological function of Mff was evaluated by loss of function and gain of function analyses, both in vitro and in vivo. RESULTS: We found that hypoxia promoted mitochondrial fission and CDDP sensitivity in HNSCC cells. Importantly, Mff was found to be correlated with chemosensitivity in primary clinical samples under hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) was found to markedly increase Mff transcription and to directly bind to Mff. Hypoxia enhanced the release of reactive oxygen species (ROS) and upregulated the expression of Mff via HIF-1α in HNSCC cells. ROS depletion in HNSCC cells attenuated HIF-1α expression, Mff expression and mitochondrial fission. Moreover, Mff knockdown led to suppression of hypoxia-induced mitochondrial fission and to decreased CDDP chemosensitivity in vivo and in vitro. CONCLUSIONS: Our findings indicate that hypoxia-induced release of ROS can promote mitochondrial fission and CDDP chemosensitivity via HIF1α/Mff regulation in HNSCC cells, indicating that Mff may serve as a biomarker to predict neoadjuvant chemosensitivity in HNSCC patients and as a target for overcoming chemoresistance.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34333980

RESUMO

The exploration of highly efficient materials for the degradation of chemical warfare agents has been a longstanding task for preventing human exposure. Herein, we report a series of metal-organic frameworks (MOFs) M-TCPP-La based on metallo-tetra(4-carboxyphenyl)porphyrin and LaIII, which were applied to selectively oxidize 2-chloroethyl ethyl sulfide (CEES, a sulfur mustard simulant) as heterogeneous photocatalysts. After irradiation from a commercial blue light-emitting diode (LED), both superoxide ion and singlet oxygen were generated by M-TCPP-La and involved in selective oxidization of CEES to 2-chloroethyl ethyl sulfoxide (CEESO). Notably, a very short half lifetime (2.5 min) was achieved using Fe-TCPP-La as the photocatalyst. In comparison to currently utilizing singlet oxygen and hydrogen peroxide as oxidizing agents, this work employing both singlet oxygen and superoxide ion represents a new and effective strategy of detoxification of mustard gas.

13.
J Plant Res ; 134(5): 1021-1035, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34218357

RESUMO

Yushania niitakayamensis (Bambusoideae; Poaceae), a perennial grass distributed from mid to high elevations in Taiwan, is often found growing in exposed grassland or shaded forest understories. To answer the question how does Y. niitakayamensis cope with contrasting light availability of habitats, we compared (1) leaf ecophysiological traits between populations of Y. niitakayamensis growing in exposed and shaded habitats in four seasons, and (2) plasticity patterns of transplanted plants to two light treatments (full-sun and 70 % shading) in a phytotron. Significant differences in leaf morphological (leaf length, width, specific leaf area, stomatal density), anatomical (leaf thickness (LT), the frequency of cavity formed by collapsed fusoid cells), and biochemical (chlorophyll contents, nitrogen contents per unit area ([N]area) and stable carbon isotope ratio) features were found between populations across seasons. Common garden experiments suggested that most of the trait variations in field growing plants can be explained by the effect of growth light treatment but not by that of population. However, variations between the two populations in LT, [N]area, gas exchange parameters, and the degree of plasticity in LT and [N]area in responding to growth light regimes might have genetic basis. In comparison between transplants from different origins grown under same light regime, plants from the exposed population grown under full-sun expressed significantly higher LT, [N]area and light-saturated photosynthetic rates, whereas plants from the shaded population grown under shading had significantly higher water use efficiency. Accordingly, local specialization in populations of Y. niitakayamensis to particular environmental conditions might have arisen.


Assuntos
Folhas de Planta , Poaceae , Adaptação Fisiológica , Variação Genética , Luz , Fotossíntese , Folhas de Planta/genética , Poaceae/genética
14.
Mol Plant ; 14(10): 1699-1713, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34216830

RESUMO

G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice, but how these pathways are coordinated in regulating this process is unknown. Here, we show that Chang Li Geng 1 (CLG1), which encodes an E3 ligase, regulates grain size by targeting the Gγ protein GS3, a negative regulator of grain length, for degradation. Overexpression of CLG1 led to increased grain length, while overexpression of mutated CLG1 with changes in three conserved amino acids decreased grain length. We found that CLG1 physically interacts with and ubiquitinats GS3which is subsequently degraded through the endosome degradation pathway, leading to increased grain size. Furthermore, we identified a critical SNP in the exon 3 of CLG1 that is significantly associated with grain size variation in a core collection of cultivated rice. This SNP results in an amino acid substitution from Arg to Ser at position 163 of CLG1 that enhances the E3 ligase activity of CLG1 and thus increases rice grain size. Both the expression level of CLG1 and the SNP CLG1163S may be useful variations for manipulating grain size in rice.

15.
Curr Opin Plant Biol ; 63: 102074, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34217918

RESUMO

The Green Revolution of the 1960s boosted cereal crop yields in part through widespread adoption of semi-dwarf plant varieties, many of which were later found to have mutations in either gibberellins (GAs) homeostasis or DELLA proteins. GA is essential for plant growth and developmental regulation and plays an important role in improving crop plant architecture for enhanced grain yield under high nitrogen conditions. A complex regulatory network governs the spatially and temporally controlled genes expression through integrative GA signaling in response to multiple endogenous and environmental cues. In this review, we summarize current advances in understanding the molecular mechanisms of DELLA-dependent and DELLA-independent GA signaling pathways and their contributions to plant developmental and metabolic adaptations to changes in nitrogen availability. The progress in molecular understanding of the plant growth-metabolic coordination will facilitate breeding strategies for future sustainable agriculture and a new Green Revolution.

16.
Sci Rep ; 11(1): 13965, 2021 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-34234195

RESUMO

Active fluid droplets surrounded by oil can spontaneously develop circulatory flows. However, the dynamics of the surrounding oil and their influence on the active fluid remain poorly understood. To investigate interactions between the active fluid and the passive oil across their interface, kinesin-driven microtubule-based active fluid droplets were immersed in oil and compressed into a cylinder-like shape. The droplet geometry supported intradroplet circulatory flows, but the circulation was suppressed when the thickness of the oil layer surrounding the droplet decreased. Experiments with tracers and network structure analyses and continuum models based on the dynamics of self-elongating rods demonstrated that the flow transition resulted from flow coupling across the interface between active fluid and oil, with a millimeter-scale coupling length. In addition, two novel millifluidic devices were developed that could trigger and suppress intradroplet circulatory flows in real time: one by changing the thickness of the surrounding oil layer and the other by locally deforming the droplet. This work highlights the role of interfacial dynamics in the active fluid droplet system and shows that circulatory flows within droplets can be affected by millimeter-scale flow coupling across the interface between the active fluid and the oil.

17.
Adv Sci (Weinh) ; 8(14): 2004319, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34306972

RESUMO

The trimethylation on histone H3 lysine 27 (H3k27me3), a transcriptionally repressive epigenetic mark of permissive chromatin, can be removed by the histone lysine demethylase 6a (Kdm6a). However, the physiological function of H3k27me3 and Kdm6a on circadian genes remains largely elusive. With the ChIP-Seq and mRNA microarray assays, a critical role is identified for Kdm6a in the regulation of H3k27me3 to impact the expression of Crytochrome 1 (Cry1) in the hypothalamus of diet induced obesity mice. More importantly, both conditional knockout and pharmacological inhibition of Kdm6a reduce body weight and stabilize blood glucose homeostasis. Although a Kdm6a inhibitor fails to decrease body weight in leptin receptor-deficient db/db mice, it significantly decreases Cry1 expression, enhances sensitivity to exogenous leptin administration, and blocks body weight increases in endo-leptin-deficient ob/ob mice. Moreover, gene analysis of the human hypothalamus further reveals a positive correlation between Kdm6a and Cry1. The results show that inhibition of Kdm6a reduces the Cry1 expression and sensitizes leptin signaling to combat obesity-related disease. Therefore, it implicates Kdm6a as an attractive drug target for obesity and metabolic disorders.

18.
Chin Med Sci J ; 36(2): 103-109, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34231458

RESUMO

Objective Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus (T2DM). Previous researches report that methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms might influence the occurrence of coronary heart disease (CHD) in T2DM patients. The purpose of this study was to evaluate whether MTHFR C677T and A1298C mutations are associated with the risk of CHD in T2DM patients. Methods A total of 197 subjects with T2DM were studied, of which 95 patients with CHD. The genotypes of MTHFR C677T and A1298C were analyzed by using dideoxy chain-termination method, and compared between patients with CHD and those without CHD. Results We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD (P=0.011). However, there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD. Furthermore, the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine (Hcy) levels (≤15 µmol/L) (P=0.006), while no effect of MTHFR gene polymorphism on the incidence of CHD was found in patients with higher Hcy levels (>15 µmol/L) (P=0.491). Conclusion The MTHFR C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hcy levels.

19.
Anal Chem ; 93(29): 10343-10350, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34264625

RESUMO

Extracellular vesicles (EVs) are small vesicles secreted by various cell types to mediate cell-to-cell communication through the transfer of macromolecules. EVs carry multiple cargo molecules that reflect the origins of their donor cells; thus, they can be considered reliable biomarkers for early cancer diagnosis. However, the diverse cellular origin of EV masks the detection signals generated by both tumor- and nontumor-derived cells. Thereby, the capability to recognize the cellular origin of EVs is the prerequisite for their diagnostic applications. In the present study, we develop an intelligent probabilistic system for tracing the cellular origin of individual EVs using single-molecule multicolor imaging. Through the analysis of the expression profile of two typical membrane protein markers, CD9 and CD63, on single EVs, accurate and rapid probabilistic recognition of EVs derived from individual tumor and nontumor cells in clinical samples is achieved. The correlation between cellular origin and surface protein phenotyping on single EVs is also exemplified. The proposed system holds great potential for advancing EVs as reliable clinical indicators and exploring their biological functions.


Assuntos
Vesículas Extracelulares , Neoplasias , Biomarcadores , Humanos , Proteínas de Membrana , Nanotecnologia
20.
PLoS Genet ; 17(7): e1009649, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34228720

RESUMO

The differentiation efficiency of adult stem cells undergoes a significant decline in aged animals, which is closely related to the decline in organ function and age-associated diseases. However, the underlying mechanisms that ultimately lead to this observed decline of the differentiation efficiency of stem cells remain largely unclear. This study investigated Drosophila midguts and identified an obvious upregulation of caudal (cad), which encodes a homeobox transcription factor. This factor is traditionally known as a central regulator of embryonic anterior-posterior body axis patterning. This study reports that depletion of cad in intestinal stem/progenitor cells promotes quiescent intestinal stem cells (ISCs) to become activate and produce enterocytes in the midgut under normal gut homeostasis conditions. However, overexpression of cad results in the failure of ISC differentiation and intestinal epithelial regeneration after injury. Moreover, this study suggests that cad prevents intestinal stem/progenitor cell differentiation by modulating the Janus kinase/signal transducers and activators of the transcription pathway and Sox21a-GATAe signaling cascade. Importantly, the reduction of cad expression in intestinal stem/progenitor cells restrained age-associated gut hyperplasia in Drosophila. This study identified a function of the homeobox gene cad in the modulation of adult stem cell differentiation and suggested a potential gene target for the treatment of age-related diseases induced by age-related stem cell dysfunction.

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