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1.
Diabetes ; 2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34103347

RESUMO

Inflammation and abnormal metabolism play important roles in the pathogenesis of diabetic nephropathy (DN). Annexin A1 (ANXA1) contributes to inflammation resolution and improves metabolism. Here, we assess the effects of ANXA1 in diabetic mice and proximal tubular epithelial cells (PTECs) treated with high glucose plus palmitate acid (HGPA), and explore the association of ANXA1 with lipid accumulation in DN patients. It is found that ANXA1 deletion aggravates renal injuries, including albuminuria, mesangial matrix expansion and tubulointerstitial lesions in HFD/STZ-induced diabetic mice. ANXA1 deficiency promotes intra-renal lipid accumulation and drives mitochondrial alterations in kidneys. In addition, Ac2-26, an ANXA1 mimetic peptide, has a therapeutic effect against lipid toxicity in diabetic mice. In HGPA-treated human PTECs, ANXA1 silencing causes FPR2/ALX-driven deleterious effects, which suppress phosphorylated Thr172AMPK, resulting in decreased PPARα and CPT1b expression and increased HGPA-induced lipid accumulation, apoptosis and elevated expression of pro-inflammatory and pro-fibrotic genes. Last but not least, the extent of lipid accumulation correlates with renal function, and the level of tubulointerstitial ANXA1 expression correlates with ectopic lipid deposition in kidneys of DN patients. These data demonstrate that ANXA1 regulates lipid metabolism of PTECs to ameliorate disease progression, hence it holds great potential as a therapeutic target for DN.

2.
Nano Lett ; 2021 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-34086465

RESUMO

For bacterial adhesion and biofilm formation, a thorough understanding of the mechanism and effective modulating is lacking due to the complex extracellular electron transfer (EET) at bacteria-surface interfaces. Here, we explore the adhesion behavior of a model electroactive bacteria under various metabolic conditions by an integrated electrochemical single-cell force microscopy system. A nonlinear model between bacterial adhesion force and electric field intensity is established, which provides a theoretical foundation for precise tuning of bacterial adhesion strength by the surface potential and the direction and flux of electron flow. In particular, based on quantitative analyses with equivalent charge distribution modeling and wormlike chain numerical simulations, it is demonstrated that the chain conformation and unfolding events of outer membrane appendages are dominantly impacted by the dynamic bacterial EET processes. This reveals how the anisotropy of bacterial conductive structure can translate into the desired adhesion behavior in different scenarios.

3.
J Colloid Interface Sci ; 600: 243-255, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-34020121

RESUMO

As a promising noninvasive tumor treatment modality, dual phototherapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), has drawn extensive research interest in imaging-guided synergistic antitumor treatment. However, developing a high-efficient phototherapeutic agent is still a huge challenge, since single photosensitizer often suffers from the insufficient photothermal conversion efficiency (PCE) or low reactive oxygen species (ROS) productivity. Moreover, the overexpression of reductive glutathione (GSH) in tumor cells also severely compromises PDT efficiency. Here, inspired by the glutathione oxidase activity of high-valent transition metal ions, we designed a copper-coordinated nanotheranostic (PhA@NanoICG) by the coordination-driven co-assembly of photothermal-agent indocyanine green (ICG) and photodynamic-agent pheophorbide A (PhA), in which Cu2+ acted as a bridge to tightly associate ICG with PhA. Such carrier-free metal-coordinated nanotheranostics exhibited ultra-high dual-photosensitizers co-loading (~96.74 wt%) and excellent structural stability. Notably, NanoICG significantly increase the PCE of ICG via J-aggregation induced UV-vis absorption red-shift. Once PhA@NanoICG accumulated in tumor sites, they could be disassembled triggered by the weakly acidic and highly reducible tumor microenvironment. Moreover, the Cu2+ can deplete intracellular GSH and impair cellular antioxidant defense system, reducing the unnecessary ROS consumption caused by glutathione. Under fluorescence/photoacoustic imaging-guided laser irradiation, local hyperthermia and ROS were generated to induce tumor cells apoptosis. The in vitro and in vivo experiments consistently confirm that PhA@NanoICG could induce remarkable tumor inhibition through self-enhanced PTT and PDT, which may pave a new way for cancer therapy.

4.
Nat Commun ; 12(1): 3242, 2021 05 28.
Artigo em Inglês | MEDLINE | ID: mdl-34050158

RESUMO

Retinal screening contributes to early detection of diabetic retinopathy and timely treatment. To facilitate the screening process, we develop a deep learning system, named DeepDR, that can detect early-to-late stages of diabetic retinopathy. DeepDR is trained for real-time image quality assessment, lesion detection and grading using 466,247 fundus images from 121,342 patients with diabetes. Evaluation is performed on a local dataset with 200,136 fundus images from 52,004 patients and three external datasets with a total of 209,322 images. The area under the receiver operating characteristic curves for detecting microaneurysms, cotton-wool spots, hard exudates and hemorrhages are 0.901, 0.941, 0.954 and 0.967, respectively. The grading of diabetic retinopathy as mild, moderate, severe and proliferative achieves area under the curves of 0.943, 0.955, 0.960 and 0.972, respectively. In external validations, the area under the curves for grading range from 0.916 to 0.970, which further supports the system is efficient for diabetic retinopathy grading.


Assuntos
Aprendizado Profundo , Retinopatia Diabética/diagnóstico , Fundo de Olho , Interpretação de Imagem Assistida por Computador/métodos , Índice de Gravidade de Doença , Idoso , Conjuntos de Dados como Assunto , Diabetes Mellitus Tipo 2/complicações , Retinopatia Diabética/etiologia , Retinopatia Diabética/patologia , Humanos , Curva ROC
5.
Sci Total Environ ; 773: 145662, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33940750

RESUMO

Biochar is a highly effective adsorbent for nitroaromatic compounds (NACs), and acts as an electron shuttle that mediates the reduction of NACs. Hence, when biochar is used to mediate NAC reduction, adsorption and reduction will occur simultaneously and affect each other. However, the effect of biochar-mediated NAC reduction on sorption remains unknown. Eight biochars with different physicochemical properties were used to adsorb m-nitrotoluene and mediate its reduction. The results showed that the adsorption of m-nitrotoluene onto the various biochars facilitated its reduction, whereas biochar-mediated reduction retarded and weakened contaminant adsorption, which increased the environmental risk posed by m-nitrotoluene. Nevertheless, biochars with a high graphitization degree and developed porosity not only had a great catalytic ability, but also significantly alleviated the negative effect of reduction on adsorption. This was ascribed to the π-π interaction and pore-filling effect, which played more important roles than the hydrophobic effect in adsorbing the reduction product (m-toluidine) onto the studied biochars during reduction. Furthermore, the methanol extraction results indicated that the eight biochars presented significantly stronger sequestration abilities for adsorbed m-toluidine than for adsorbed m-nitrotoluene. This resulted from the hydrogen bonding and the Lewis acid-base effect between m-toluidine and each biochar, which were absent for m-nitrotoluene. These results suggest that biochars with a high graphitization degree and developed porosity are applicable for mediating reduction-enhancing sequestration of NACs, which could be a novel strategy for NAC remediation.


Assuntos
Carvão Vegetal , Tolueno , Adsorção , Tolueno/análogos & derivados
6.
Comput Intell Neurosci ; 2021: 5577956, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34054939

RESUMO

Magnetic resonance (MR) images often suffer from random noise pollution during image acquisition and transmission, which impairs disease diagnosis by doctors or automated systems. In recent years, many noise removal algorithms with impressive performances have been proposed. In this work, inspired by the idea of deep learning, we propose a denoising method named 3D-Parallel-RicianNet, which will combine global and local information to remove noise in MR images. Specifically, we introduce a powerful dilated convolution residual (DCR) module to expand the receptive field of the network and to avoid the loss of global features. Then, to extract more local information and reduce the computational complexity, we design the depthwise separable convolution residual (DSCR) module to learn the channel and position information in the image, which not only reduces parameters dramatically but also improves the local denoising performance. In addition, a parallel network is constructed by fusing the features extracted from each DCR module and DSCR module to improve the efficiency and reduce the complexity for training a denoising model. Finally, a reconstruction (REC) module aims to construct the clean image through the obtained noise deviation and the given noisy image. Due to the lack of ground-truth images in the real MR dataset, the performance of the proposed model was tested qualitatively and quantitatively on one simulated T1-weighted MR image dataset and then expanded to four real datasets. The experimental results show that the proposed 3D-Parallel-RicianNet network achieves performance superior to that of several state-of-the-art methods in terms of the peak signal-to-noise ratio, structural similarity index, and entropy metric. In particular, our method demonstrates powerful abilities in both noise suppression and structure preservation.

7.
J Am Chem Soc ; 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33929193

RESUMO

Axially, epitaxially organizing nano-objects of distinct compositions and structures into superlattice nanowires enables full utilization of sunlight, readily engineered band structures, and tunable geometric parameters to fit carrier transport, thus holding great promise for optoelectronics and solar-to-fuel conversion. To maximize their efficiency, the general and high-precision synthesis of colloidal axial superlattice nanowires (ASLNWs) with programmable compositions and structures is the prerequisite; however, it remains challenging. Here, we report an axial encoding methodology toward the ASLNW library with precise control over their compositions, dimensions, crystal phases, interfaces, and periodicity. Using a predesigned, editable nanoparticle framework that offers the synthetic selectivity, we are able to chemically decouple adjacent sub-objects in ASLNWs and thus craft them in a controlled approach, yielding a library of distinct ASLNWs. We integrate therein plasmonic, metallic, or near-infrared-active chalcogenides, which hold great potential in solar energy conversion. Such synthetic capability enables a performance boost in target applications, as we report order-of-magnitude enhanced photocatalytic hydrogen production rates using optimized ASLNWs compared to corresponding solo objects. Furthermore, it is expected that such unique superlattice nanowires could bring out new phenomena.

8.
Artigo em Inglês | MEDLINE | ID: mdl-33793182

RESUMO

Ultrathin freestanding membranes with a pronounced metal-insulator transition (MIT) have huge potential for future flexible electronic applications as well as provide a unique aspect for the study of lattice-electron interplay. However, the reduction of the thickness to an ultrathin region (a few nm) is typically detrimental to the MIT in epitaxial films, and even catastrophic for their freestanding form. Here, we report an enhanced MIT in VO2-based freestanding membranes, with a lateral size up to millimeters and the VO2 thickness down to 5 nm. The VO2 membranes were detached by dissolving a Sr3Al2O6 sacrificial layer between the VO2 thin film and the c-Al2O3(0001) substrate, allowing the transfer onto arbitrary surfaces. Furthermore, the MIT in the VO2 membrane was greatly enhanced by inserting an intermediate Al2O3 buffer layer. In comparison with the best available ultrathin VO2 membranes, the enhancement of MIT is over 400% at a 5 nm VO2 thickness and more than 1 order of magnitude for VO2 above 10 nm. Our study widens the spectrum of functionality in ultrathin and large-scale membranes and enables the potential integration of MIT into flexible electronics and photonics.

9.
Nat Nanotechnol ; 2021 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-33875873

RESUMO

Antiferromagnets are interesting materials for spintronics because of their faster dynamics and robustness against perturbations from magnetic fields. Control of the antiferromagnetic order constitutes an important step towards applications, but has been limited to bulk materials so far. Here, using spatially resolved second-harmonic generation, we show direct evidence of long-range antiferromagnetic order and Ising-type Néel vector switching in monolayer MnPSe3 with large XY anisotropy. In additional to thermally induced switching, uniaxial strain can rotate the Néel vector, aligning it to a general in-plane direction irrespective of the crystal axes. A change of the universality class of the phase transition in the XY model under uniaxial strain causes this emergence of strain-controlled Ising order in the XY magnet MnPSe3. Our discovery is a further ingredient for compact antiferromagnetic spintronic devices in the two-dimensional limit.

10.
Langmuir ; 37(18): 5548-5553, 2021 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-33904742

RESUMO

Hybrid core-shell nanoparticles play a very significant role in many applications. Here, we report a light-induced oligomer coating on nanoparticles via Norrish type I reaction. The radical species generated via UV irradiation can chemically initiate the photoinitiators, which are then polymerized and deposited on inorganic nanoparticles via heterogeneous nucleation, forming a soft oligomer coating smaller than 40 nm. This coating method is versatile and potentially applicable to many different types of inorganic cores and their assemblies, making it a very useful technique for "freezing" nanoassemblies in solution. Moreover, these oligomer coatings containing radical species can also initiate surface polymerization of both styrenic and acrylic monomers with certain functionalities for different applications such as self-assembly, plasmon tuning, and pH sensing (3.5-4.5).

11.
J Colloid Interface Sci ; 594: 531-539, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33774409

RESUMO

Herein we develop a novel and effective alkoxide hydrolysis approach to in-situ construct the trimanganese tetraoxide (Mn3O4)/graphene nanostructured composite as high-performance anode material for lithium-ion batteries (LIBs). This is the first report on the synthesis of Mn3O4/graphene composite via a facile hydrolysis of the manganese alkoxide (Mn-alkoxide)/graphene precursor. Before hydrolysis, two dimensional (2D) Mn-alkoxide nanoplates are closely adhered to 2D graphene nanosheets via Mn-O chemical bonding. After hydrolysis, the Mn-alkoxide in-situ converts to Mn3O4, while the Mn-O bond is preserved. This leads to a robust Mn3O4/graphene hybrid architecture with 15 nm Mn3O4 nanocrystals homogeneously anchoring on graphene nanosheets. This not only prevents the Mn3O4 nanocrystals agglomeration but also inversely mitigates the graphene nanosheets restacking. Moreover, the flexible and conductive graphene nanosheets can accommodate the volume change. This maintains the structural and electrical integrity of the Mn3O4/graphene electrode during the cycling process. As a result, the Mn3O4/graphene composite displays superior lithium storage performance with high reversible capacity (741 mAh g-1 at 100 mA g-1), excellent rate capability (403 mAh g-1 at 1000 mA g-1) and long cycle life (527 mAg g-1 after 300 cycles at 500 mA g-1). The electrochemical performance highlights the importance of rational design nanocrystals anchoring on graphene nanosheets for high-performance LIBs application.

12.
Mediators Inflamm ; 2021: 8086253, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33688304

RESUMO

Patients being treated for pulmonary tuberculosis often suffer liver injury due to the effects of anti-TB drugs, and the underlying mechanisms for those injuries need to be clarified. In this study, rats and hepatic cells were administrated isoniazid (INH) and rifampin (RIF) and then treated with NLRP3-inflammasome inhibitors (INF39 and CP-456773) or NLRP3 siRNA. Histopathological changes that occurred in liver tissue were examined by H&E staining. Additionally, the levels IL-33, IL-18, IL-1ß, NLRP3, ASC, and cleaved-caspase 1 expression in the liver tissues were also determined. NAT2 and CYP2E1 expression were identified by QRT-PCR analysis. Finally, in vitro assays were performed to examine the effects of siRNA targeting NLRP3. Treatment with the antituberculosis drugs caused significant liver injuries, induced inflammatory responses and oxidative stress (OS), activated NLRP3 inflammasomes, reduced the activity of drug-metabolizing enzymes, and altered the antioxidant defense system in rats and hepatic cells. The NLRP3 inflammasome was required for INH- and RIF-induced liver injuries that were produced by inflammatory responses, OS, the antioxidant defense system, and drug-metabolizing enzymes. This study indicated that the NLRP3 inflammasome is involved in antituberculosis drug-induced liver injuries (ATLIs) and suggests NLRP3 as a potential target for attenuating the inflammation response in ATLIs.

13.
J Voice ; 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33752927

RESUMO

OBJECTIVE: While the vocal fold is in direct contact anteriorly with the thyroid cartilage, posteriorly the vocal fold connects to the thyroid cartilage through a soft tissue layer in the paraglottic space. Currently the paraglottic space is often neglected in computational models of phonation, in which a fixed boundary condition is often imposed on the lateral surface of the vocal fold. The goal of this study was to investigate the effect of the paraglottic space on voice production in an MRI-based vocal fold model, and how this effect may be counteracted by vocal fold stiffening due to laryngeal muscle activation. METHODS: Parametric simulation study using an MRI-based computational vocal fold model. RESULTS: The results showed that the presence of the paraglottic space increased the mean and amplitude of the glottal area waveform, decreased the phonation frequency and closed quotient. For the particular vocal fold geometry used in this study, the presence of the paraglottic space also reduced the occurrence of irregular vocal fold vibration. These effects of the paraglottic space became smaller with increasing paraglottic space stiffness and to a lesser degree with vocal fold stiffening. CONCLUSIONS: The results suggest that the paraglottic space may be neglected in qualitative evaluations of normal phonation, but needs to be included in simulations of pathological phonation or vocal fold posturing.

14.
Technol Health Care ; 29(S1): 367-376, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33682774

RESUMO

BACKGROUND: The delayed blood pressure recovery (BPR) at post-exercise has been in association with a major risk of cardiovascular disease and death. OBJECTIVE: The study focused on evaluating the systolic and diastolic blood pressure recovery (SBPR, DBPR) and the autonomic modulation following treadmill exercise in healthy young adults. Although considerable literature had been published about BPR and HRV, the association between BPR and ultra-short-term HRV has not yet been completely described. METHODS: Fifteen subjects performed exercise with three different intensities on a treadmill, the speed was 6 km/h, 9 km/h, 12 km/h, respectively. SBP and DBP was measured per 30 s in each trial. The synchronous 5-min electrocardiogram (ECG) signals were recorded and HRV30s parameters including SDNN30s, RMSSD30s, SDNN30s/RMSSD30s, SD130s, SD230s and SD130s/SD230s were calculated every 30 s periods in 5 min ECG signals to match the corresponding BPR. RESULTS: The intraclass correlation coefficient (ICC) values and the Bland-Altman plots indicated good consistency and repeatability between HRV30s and HRV5min at three post-exercise trials, with most ICC values > 0.75. Besides, SBPR and DBPR generally decreased and returned to the Rest level in 5 mins. The Spearman correlation coefficients showed strong relationships between BPR and HRV30s sympathetic-vagal balance parameters, i.e., ratio SDNN/RMSSD and ratio SD1/SD2. CONCLUSIONS: These observations represented a new insight into the cardiovascular regulation at post-exercise, which could contribute to physical exercise areas in the future.

15.
BMC Bioinformatics ; 22(1): 138, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33752602

RESUMO

BACKGROUND: The rapidly increasing dimensionality and throughput of flow and mass cytometry data necessitate new bioinformatics tools for analysis and interpretation, and the recently emerging single-cell-based algorithms provide a powerful strategy to meet this challenge. RESULTS: Here, we present CytoTree, an R/Bioconductor package designed to analyze and interpret multidimensional flow and mass cytometry data. CytoTree provides multiple computational functionalities that integrate most of the commonly used techniques in unsupervised clustering and dimensionality reduction and, more importantly, support the construction of a tree-shaped trajectory based on the minimum spanning tree algorithm. A graph-based algorithm is also implemented to estimate the pseudotime and infer intermediate-state cells. We apply CytoTree to several examples of mass cytometry and time-course flow cytometry data on heterogeneity-based cytology and differentiation/reprogramming experiments to illustrate the practical utility achieved in a fast and convenient manner. CONCLUSIONS: CytoTree represents a versatile tool for analyzing multidimensional flow and mass cytometry data and to producing heuristic results for trajectory construction and pseudotime estimation in an integrated workflow.


Assuntos
Algoritmos , Biologia Computacional , Diferenciação Celular , Análise por Conglomerados , Citometria de Fluxo , Software
16.
Artigo em Inglês | MEDLINE | ID: mdl-33739910

RESUMO

The Intensive Supervision Mechanism (hereafter referred to as ISM) is one of the most important institutional management innovations for air pollution control in China, but there is currently no consensus on the effects of the ISM on air quality improvement. In this study, a reliable quantitative model based on the Difference-in-Differences (DID) analysis was designed to evaluate the impacts of ISM on air quality (as indicated by good air quality days (hereafter referred to as GAD) and the concentrations of six major air pollutants (i.e. PM2.5, PM10, O3_8H, NO2, SO2, and CO)), in China with focuses on the implementation cities of Henan Province. To optimize the model design, six meteorological factors, five socio-economic indicators, and VIIRS (Visible Infrared Imaging Radiometer Suite) data were also considered as alternative control variables for more comprehensive and effective results. In addition, the redundancy analysis (RDA) and Monte Carlo simulation were conducted to determine the optimal combination of those control variables which can best reflect the changes in explanatory variables. The main findings are as follows: (1) the statistical model applied in this study can well evaluate the impacts of ISM; (2) the implementation of ISM can significantly reduce the concentrations of SO2, CO, and NO2, but the improvements for PM2.5, PM10, GAD and O3_8H were not significant. (3) the potential for air quality improvement due to ISM tends to be reduced over time, and thus the positive effects of ISM at its second stage were not increased significantly compared with those observed during its first stage. In general, those results not only demonstrate the effectiveness of ISM on air quality improvement, but also provide insights into how the ISM can be optimized to gain a sustained improvement of the ambient air quality in the future.

17.
Sci Rep ; 11(1): 5325, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33674641

RESUMO

Recent advances in single-cell RNA sequencing (scRNA-seq) have improved our understanding of the association between tumor-infiltrating lymphocyte (TILs) heterogeneity and cancer initiation and progression. However, studies investigating alternative splicing (AS) as an important regulatory factor of heterogeneity remain limited. Here, we developed a new computational tool, DESJ-detection, which accurately detects differentially expressed splicing junctions (DESJs) between cell groups at the single-cell level. We analyzed 5063 T cells of hepatocellular carcinoma (HCC) and identified 1176 DESJs across 11 T cell subtypes. Interestingly, DESJs were enriched in UTRs, and have putative effects on heterogeneity. Cell subtypes with a similar function closely clustered together at the AS level. Meanwhile, we identified a novel cell state, pre-activation with the isoform markers ARHGAP15-205. In summary, we present a comprehensive investigation of alternative splicing differences, which provided novel insights into T cell heterogeneity and can be applied to other full-length scRNA-seq datasets.

18.
Kidney Int ; 2021 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-33675846

RESUMO

Since failed resolution of inflammation is a major contributor to the progression of diabetic nephropathy, identifying endogenously generated molecules that promote the physiological resolution of inflammation may be a promising therapeutic approach for this disease. Annexin A1 (ANXA1), as an endogenous mediator, plays an important role in resolving inflammation. Whether ANXA1 could affect established diabetic nephropathy through modulating inflammatory states remains largely unknown. In the current study, we found that in patients with diabetic nephropathy, the levels of ANXA1 were upregulated in kidneys, and correlated with kidney function as well as kidney outcomes. Therefore, the role of endogenous ANXA1 in mouse models of diabetic nephropathy was further evaluated. ANXA1 deficiency exacerbated kidney injuries, exhibiting more severe albuminuria, mesangial matrix expansion, tubulointerstitial lesions, kidney inflammation and fibrosis in high fat diet/streptozotocin-induced-diabetic mice. Consistently, ANXA1 overexpression ameliorated kidney injuries in mice with diabetic nephropathy. Additionally, we found Ac2-26 (an ANXA1 mimetic peptide) had therapeutic potential for alleviating kidney injuries in db/db mice and diabetic Anxa1 knockout mice. Mechanistic studies demonstrated that intracellular ANXA1 bound to the transcription factor NF-κB p65 subunit, inhibiting its activation thereby modulating the inflammatory state. Thus, our data indicate that ANXA1 may be a promising therapeutic approach to treating and reversing diabetic nephropathy.

19.
Ann Palliat Med ; 10(1): 244-249, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33545762

RESUMO

BACKGROUND: Sepsis is an important disease that endangers human health and is the main cause of death in ICU patients, which has been a focus of clinical treatment. This study aims to evaluate the significance of the readily available quick sequential organ failure assessment (qSOFA) score in clinical cases of sepsis. METHODS: A retrospective cross-sectional study of patients with sepsis treated in the Department of Infectious Diseases, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology from January 2015 to December 2016 was conducted, and the patients were divided into a high-score group (≥2 points) and the low-score group (<2 points) according to the diagnostic criteria for sepsis (Sepsis 3.0). The differences in disease outcome and inflammatory indicators were compared between groups. RESULTS: A total of 74 patients with sepsis were included in this study. When the cutoff qSOFA score was 2 points, the patients in the high-score group showed a higher mortality rate (71.43%), and the patients in the low-score group showed a higher improvement rate (87.76%). The inflammatory indicators did not show statistically significant differences between the two groups. CONCLUSIONS: The qSOFA score can better predict the prognoses of non-ICU patients with sepsis compared with traditional inflammatory indicators. Clinicians should raise their awareness about qSOFA and improving its accuracy.


Assuntos
Escores de Disfunção Orgânica , Sepse , Estudos Transversais , Mortalidade Hospitalar , Humanos , Prognóstico , Estudos Retrospectivos , Sepse/diagnóstico
20.
Nano Lett ; 21(6): 2690-2698, 2021 03 24.
Artigo em Inglês | MEDLINE | ID: mdl-33543616

RESUMO

Although injectable hydrogel microsphere has demonstrated tremendous promise in clinical applications, local overactive inflammation in degenerative diseases could jeopardize biomaterial implantation's therapeutic efficacy. Herein, an injectable "peptide-cell-hydrogel" microsphere was constructed by covalently coupling of APETx2 and further loading of nucleus pulposus cells, which could inhibit local inflammatory cytokine storms to regulate the metabolic balance of ECM in vitro. The covalent coupling of APETx2 preserved the biocompatibility of the microspheres and achieved a controlled release of APETx2 for more than 28 days in an acidic environment. By delivering "peptide-cell-hydrogel" microspheres to a rat degenerative intervertebral disc at 4 weeks, the expression of ASIC-3 and IL-1ß was significantly decreased for 3.53-fold and 7.29-fold, respectively. Also, the content of ECM was significantly recovered at 8 weeks. In summary, the proposed strategy provides an effective approach for tissue regeneration under overactive inflammatory responses.

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