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1.
Food Chem ; 366: 130584, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34293541

RESUMO

Monofloral safflower honey (MSH), produced from nectar of the medicinal Carthamus tinctorius L., has been shown with excellent nutritional value and biological activity. However, current MSH authenticity verification is insufficient. Herein, we fully characterized MSH from a metabolomic perspective and proposed a chemical marker for its authentication. Using palynological analysis, we confirmed the botanical origin of MSH. Ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was applied further to compare MSH/safflower components. MSH and safflowers shared 1297 tentatively identified compounds, of which safflomin A was identified as a reliable characteristic indicator. When applied to commercial non-safflower honeys, none tested safflomin A positive. Solid phase extraction coupled UHPLC/Q-TOF-MS method revealed the LOD and LOQ of safflomin A in MSH to be 0.006 and 0.02 mg/kg, respectively, with concentrations ranging from 0.86 to 3.91 mg/kg. Collectively, safflomin A can be applied as a chemical marker for fingerprinting the botanical origin of safflower honey.


Assuntos
Carthamus tinctorius , Mel , Carthamus tinctorius/genética , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos , Glucosídeos , Mel/análise , Espectrometria de Massas
2.
Artigo em Inglês | MEDLINE | ID: mdl-34532933

RESUMO

Current nonlinear optical materials face a conventional limitation in the trade-off between the band gap and birefringence, especially in the deep UV spectral region. To circumvent this dilemma, we propose a general principle, π-conjugated confinement, to partially decouple the interunit π-conjugated interactions by the separation of non-π-conjugated units. The goal is to further enlarge the band gap to a value larger than that of the singular π-conjugated counterpart and to maintain a suitable density of π-conjugated units to gain a large optical anisotropy. We reveal that π-conjugated confinement is a shared structural feature for all DUV NLO materials known to date, and thus, it provides a novel and essential design criterion for future design synthesis. Guided by this principle, the carbonophosphates are predicted to be a new promising DUV candidate system. Sr3 Y[PO4 ][CO3 ]3 (1) and Na3 X[PO4 ][CO3 ] (X=Ba, Sr, Ca, Mg, 2-5) exhibit not only greatly enhanced birefringence that is 3-24 times larger than that of singular phosphates but also enhanced band gaps that are 0.2-1.7 eV wider than those of singular carbonates.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34410701

RESUMO

The approach to constructing proton transport channels via direct adjustments, including hydrophilia and analytical acid concentration in hydrophilic domains, has been proved to be circumscribed when encouraging the flatter hydrophilic-hydrophobic microphase separation structures and reducing conductivity activation energy. Here, we propose a constructive solution by regulating the polarity of hydrophobic domains, which indirectly varies the aggregation and connection of hydrophilic ion clusters during membrane formation, enabling orderly self-assembly and homogeneously distributed microphase structures. Accordingly, a series of comb-shaped polymers were synthesized with diversified optimization, and more uniformly distributed ion cluster lattices were subsequently observed using high-resolution transmission electron microscopy. Simultaneously, combining with density functional theory calculations, we analyzed the mechanism of membrane degradations caused by hydroxyl radical attacks. Experimental results demonstrated that, facilitated by proper molecule polarity, beneficial changes of bond dissociation energy could extend the membrane lifetime more than the protection from side chains near ether bonds, which were deemed to reduce the probability of attacks by the steric effect. With the optimal strategy chosen among various trials, the maximum power density of direct methanol fuel cell and H2/air proton exchange membrane fuel cell was enhanced to 95 and 485 mW cm-2, respectively.

4.
Nat Commun ; 12(1): 4755, 2021 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-34362890

RESUMO

Some specific chemotherapeutic drugs are able to enhance tumor immunogenicity and facilitate antitumor immunity by inducing immunogenic cell death (ICD). However, tumor immunosuppression induced by the adenosine pathway hampers this effect. In this study, E-selectin-modified thermal-sensitive micelles are designed to co-deliver a chemotherapeutic drug (doxorubicin, DOX) and an A2A adenosine receptor antagonist (SCH 58261), which simultaneously exhibit chemo-immunotherapeutic effects when applied with microwave irradiation. After intravenous injection, the fabricated micelles effectively adhere to the surface of leukocytes in peripheral blood mediated by E-selectin, and thereby hitchhiking with leukocytes to achieve a higher accumulation at the tumor site. Further, local microwave irradiation is applied to induce hyperthermia and accelerates the release rate of drugs from micelles. Rapidly released DOX induces tumor ICD and elicits tumor-specific immunity, while SCH 58261 alleviates immunosuppression caused by the adenosine pathway, further enhancing DOX-induced antitumor immunity. In conclusion, this study presents a strategy to increase the tumor accumulation of drugs by hitchhiking with leukocytes, and the synergistic strategy of chemo-immunotherapy not only effectively arrested primary tumor growth, but also exhibited superior effects in terms of antimetastasis, antirecurrence and antirechallenge.


Assuntos
Tratamento Farmacológico , Imunoterapia , Leucócitos/efeitos dos fármacos , Micelas , Neoplasias/terapia , Animais , Doxorrubicina/farmacologia , Portadores de Fármacos/administração & dosagem , Liberação Controlada de Fármacos , Feminino , Hipertermia/terapia , Imunidade , Camundongos , Camundongos Endogâmicos BALB C , Micro-Ondas/uso terapêutico , Fototerapia
5.
Am J Clin Dermatol ; 2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34374027

RESUMO

BACKGROUND: Atopic dermatitis is a chronic, inflammatory condition causing a substantial burden to patients and caregivers. SHR0302 is an oral, highly selective, Janus kinase 1 inhibitor under investigation for inflammatory skin diseases. OBJECTIVE: The aim of this study was to investigate the efficacy and safety of SHR0302 in Chinese patients with moderate to severe atopic dermatitis. DESIGN AND SETTING: A randomized, double-blind, placebo-controlled, multicenter, phase II trial was conducted in China between October 2019 and August 2020. PARTICIPANTS: Patients (n = 105) aged 18-75 years with moderate to severe dermatitis and nonresponsive or intolerant to topical or conventional systemic treatments were included. INTERVENTIONS: Patients were randomly assigned in a ratio of 1:1:1 to receive SHR0302 4 mg once daily, SHR0302 8 mg once daily, or placebo for 12 weeks. MAIN OUTCOME MEASURES: The primary efficacy endpoint was the proportion of patients achieving Investigator's Global Assessment (IGA) response (IGA of 0 [clear] or 1 [almost clear] with improvement of ≥2 grades) at week 12. Secondary efficacy assessments included Eczema Area and Severity Index (EASI) and pruritus Numerical Rating Scale (NRS) scores. RESULTS: At week 12, IGA response was achieved in nine patients (25.7%; 90% confidence interval [CI] 13.6-37.9%; p = 0.022) in the SHR0302 4 mg group, 19 patients (54.3%; 90% CI 40.4-68.1%; p < 0.001) in the SHR0302 8 mg group, and two patients (5.7%; 90% CI 0.0-12.2%) in the placebo group. EASI75 was achieved in 51.4% (p = 0.013), 74.3% (p < 0.001), and 22.9% of patients in the SHR0302 4 mg, SHR0302 8 mg, and placebo groups, respectively, while an NRS ≥3-point improvement occurred in 65.7% (p < 0.001), 74.3% (p < 0.001), and 22.9% of patients, respectively. Treatment-emergent adverse events were reported in 60.0%, 68.6%, and 51.4% of patients in the SHR0302 4 mg, SHR0302 8 mg, and placebo groups, respectively. The adverse events were mild in most cases. Three serious adverse events were reported, all being worsening of atopic dermatitis. No serious infection was reported. CONCLUSIONS AND RELEVANCE: Oral SHR0302 was effective and well tolerated in Chinese adult patients with moderate to severe atopic dermatitis. TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT04162899; URL: https://clinicaltrials.gov/ . Date first registered: 14 November 2019.

6.
Angew Chem Int Ed Engl ; 60(39): 21216-21220, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34268842

RESUMO

Over recent decades, guided by the anionic group theory of nonlinear optical (NLO) materials, rational design strategies have been primarily focused on anionic moieties; consequently, structural modification and design of cationic moieties have long been neglected. Herein, we report a strategy for the coordination of cationic moieties that substantially enhances the optical properties of NLO materials. For an example with well-known crystal structure, [Ag(NH3 )2 ]2 SO4 , we demonstrate that the coordination of the Ag+ cation by the neutral ligand drives the formation of a noncentrosymmetric tetragonal P 4 ‾ 21 c structure as a positive uniaxial crystal. The bending of the [Ag(NH3 )2 ]+ cationic moiety parallel to the z-axis generates an anisotropic arrangement of the dipoles, i.e., a dipole of 0.12 D along the z-direction, yet zero dipole in the xy-plane, which interacts anisotropically with the incident light oscillating electromagnetic wave, leading to optical anisotropy with a large birefringence. The incident beam of 589.3 nm normal to the (110) crystal plane measures Δnobv. =0.08, and [Ag(NH3 )2 ]2 SO4 also exhibits a phase-matching NLO response 1.4 times that of KH2 PO4 (KDP) (obv. 1.4×KDP @1064 nm; cal. d36 =1.50 pm V-1 ).

7.
Front Immunol ; 12: 671150, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34276660

RESUMO

Plant polyphenols are rich sources of natural anti-oxidants and prebiotics. After ingestion, most polyphenols are absorbed in the intestine and interact with the gut microbiota and modulated metabolites produced by bacterial fermentation, such as short-chain fatty acids (SCFAs). Dietary polyphenols immunomodulatory role by regulating intestinal microorganisms, inhibiting the etiology and pathogenesis of various diseases including colon cancer, colorectal cancer, inflammatory bowel disease (IBD) and colitis. Foodomics is a novel high-throughput analysis approach widely applied in food and nutrition studies, incorporating genomics, transcriptomics, proteomics, metabolomics, and integrating multi-omics technologies. In this review, we present an overview of foodomics technologies for identifying active polyphenol components from natural foods, as well as a summary of the gastrointestinal protective effects of polyphenols based on foodomics approaches. Furthermore, we critically assess the limitations in applying foodomics technologies to investigate the protective effect of polyphenols on the gastrointestinal (GI) system. Finally, we outline future directions of foodomics techniques to investigate GI protective effects of polyphenols. Foodomics based on the combination of several analytical platforms and data processing for genomics, transcriptomics, proteomics and metabolomics studies, provides abundant data and a more comprehensive understanding of the interactions between polyphenols and the GI tract at the molecular level. This contribution provides a basis for further exploring the protective mechanisms of polyphenols on the GI system.

8.
Ecotoxicol Environ Saf ; 220: 112379, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34058677

RESUMO

Sulfoxaflor is a novel sulfoximine insecticide which is widely used to control crop pests. Risk assessments have reported its high toxicity to pollinators. However, sulfoxaflor is not persistent in the environment and few studies have addressed its negative effects on larval and newly emerged honeybees at environmentally relevant concentrations. In the present study, the sublethal effects of a sulfoxaflor commercial product, Isoclast™ Active, were evaluated in the laboratory using larvae and newly emerged worker honeybees. The results of 96-h acute toxicity showed that Isoclast is moderately toxic to adult bees, and it could induce significant death and growth failure of larvae after continuous dietary intake. In addition, Isoclast induced significant changes in antioxidative (SOD, CAT), lipid peroxidation (POD, LPO, MDA), detoxification (GST, GR, GSH) and signal transduction-related (AChE, ACh) enzymes or products both in larvae and adult honey bees under residue levels. Here we firstly reported the lethal and sublethal effects of commercial sulfoxaflor to honeybees' larvae and young workers. All these findings revealed the potential risks of sulfoxaflor residue in environment to honey bees, and may also to other pollinators. This is a laboratory mimic studies, and further studies are still needed to investigate the risks and in-depth mechanisms of sulfoxaflor to bees in field.


Assuntos
Abelhas/efeitos dos fármacos , Exposição Ambiental/efeitos adversos , Inseticidas/toxicidade , Larva/efeitos dos fármacos , Piridinas/toxicidade , Compostos de Enxofre/toxicidade , Animais , Dieta , Estresse Oxidativo , Polinização , Água
9.
Ying Yong Sheng Tai Xue Bao ; 32(5): 1890-1902, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34042386

RESUMO

Root rot is a serious soil-borne disease, with negative consequences on crop yield and quality. Arbuscular mycorrhizal (AM) fungi are a group of soil microorganisms, which play important physiological and ecological functions by establishing symbionts with plant roots. AM fungi could induce plant resistance against root rot by regulating physiological and biochemical processes. As a biological agent, AM fungi are used to antagonize soil-borne diseases such as root rot, which is a hotspot in the field of plant-microorganism interaction. We comprehensively reviewed the suppression effect of AM fungi on plant root rot, and the effect of AM fungi on root morphology of host plant, plant nutrition levels, as well as their role in competing with pathogens for ecological sites, activating plant defense systems, and regulating root exudates. Finally, we discussed the potential mechanism of AM fungi inhibiting root rot, as well as the practical problems in the efficient utilization of AM fungi were discussed, in order to provide the theoretical basis for the biological control protocol to antagonize root rot with AM fungi.


Assuntos
Micorrizas , Fungos , Raízes de Plantas , Plantas , Solo
10.
Hum Genomics ; 15(1): 21, 2021 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-33845897

RESUMO

BACKGROUND: Non-small cell lung carcinoma (NSCLC) is one of the most common human cancers, comprising approximately 80-85% of all lung carcinomas. An estimated incidence of NSCLC is approximately 2 million new cases per year worldwide. RESULTS: In recent decade, the treatment of NSCLC has made breakthrough progress owing to a large number of targeted therapies which were approved for clinical use. Epidemiology, genetic susceptibility, and molecular profiles in patients are likely to play an important factor in response rates and survival benefits to these targeted treatments and thus warrant further investigation on ethnic differences in NSCLC. In this study, a total number of 1500 Chinese patient samples,1000 formalin fixed paraffin-embedded (FFPE) and 500 blood samples, from patients with NSCLC were analyzed by targeted sequencing to explore mutational landscape in ethnic groups associated with China. CONCLUSIONS: Overall, the data presented here provide a comprehensive analysis of NSCLC mutational landscape in Chinese patients and findings are discussed in the context of similar studies on different ethnic groups.

11.
Food Chem ; 354: 129568, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33799063

RESUMO

The identification of geographical origin is an important factor in evaluating the authenticity of honey. However, at present, there are few studies concerning the honey of Apis cerana Fabricius (A. cerana, Asiatic honeybee). To identify geographical origin, we used two common methods (multi-physicochemical parameters and phenolic chromatographic fingerprints) but achieved only poor identification. To compensate for this shortcoming, we established an ICP-MS-based ionomics method using 18 elements in 27 A. cerana honey samples from three different areas in Shaanxi Province, China. Multivariate analysis showed that significant differences in contents can be used to discriminate the geographical origin of A. cerana honey. The method was further validated using an independent test set of 11 samples with 90.91% accuracy, demonstrating its potential for the identification and prediction of the geographical origin of honey.


Assuntos
Abelhas , Análise de Alimentos/métodos , Geografia , Mel/análise , Espectrometria de Massas , Animais , Análise Espectral
12.
J Am Chem Soc ; 143(9): 3647-3654, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33646792

RESUMO

We herein report a novel semiorganic NLO material, (C5H6ON)+(H2PO4)-, 4HPP, showing promising excellent properties in the important solar-blind UV region where LAP and its deuterated form DLAP are the only commercialized semiorganic materials. For the first time, the 4-hydroxypyridine (4HP+, (C5H6ON)+) cation is identified as NLO active and how to eliminate the dipole-dipole interaction to avoid the unwanted center-symmetry-trap caused by the polar-induced susceptibility is well demonstrated. Remarkably, 4HPP exhibits competitive and even better properties compared with LAP that include better thermal stability (decomposition at 166 vs 112 °C of LAP); wider transparency range (0.26-1.50 µm); very strong SHG response (3 × KDP); a suitable large birefringence (Δncal = 0.25 vs 0.075 of LAP); and a high laser-induced damage threshold (2.2 × KDP). First-principles calculations show that the π-conjugated organic (4HP)+ cation governs the optical anisotropy, whereas the synergy of the organic and inorganic moieties dominates the SHG process. Our discovery points out a new path for the rational design of high performance semiorganic materials that require an acentric structure.

13.
Food Chem ; 352: 129312, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-33652193

RESUMO

High-value acacia honey is often adulterated with inexpensive high fructose corn syrup (HFCS), due to their similar color and sugar composition. α­Dicarbonyl compounds formed by Maillard reaction or caramelization during heat treatment or storage, differ between HFCS and honey due to differences in starting materials and processing methods. In this study, we compared α-dicarbonyl compounds in acacia honey and HFCS by Ion Mobility-Mass Spectrometry and multivariate statistical analysis. Through α-dicarbonyl compound derivatization with o-phenylenediamine, we screened a marker with 189.1023 m/z and 139.3 Å2 Collision Cross-Section that can distinguish HFCS from acacia honey. Nuclear magnetic resonance spectra identified this marker compound as 3,4-dideoxypentosulose. We then used chromatography-coupled tandem mass spectrometry to quantitate 3,4-dideoxypentosulose in market samples of honey and HFCS and found that 3,4-dideoxypentosulose was negligible (<0.098 mg/kg) in honey, but prevalent in HFCS (≧1.174 mg/kg), indicating 3,4-dideoxypentosulose can serve as an alternative indicator of HFCS adulteration of acacia honey.


Assuntos
Acacia/química , Bebidas/análise , Cromatografia Líquida de Alta Pressão , Análise de Alimentos/métodos , Xarope de Milho Rico em Frutose/análise , Espectrometria de Mobilidade Iônica , Espectrometria de Massas em Tandem , Qualidade dos Alimentos , Fraude/prevenção & controle
14.
Chem Commun (Camb) ; 57(29): 3615-3618, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33724281

RESUMO

In this paper, we found that (NH4)2V4O9 undergoes an electrochemical activation process in the first charging process at ∼1.4 V (vs. Zn2+/Zn), leading to a significant improvement of capacity and cycling stability. The activated vanadium oxides delivered a high specific capacity of 477 mA h g-1 at 50 mA g-1 and outstanding cycling stability with 97.7% capacity retention after 5000 cycles at 15 A g-1.

15.
J Agric Food Chem ; 69(11): 3362-3370, 2021 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-33688728

RESUMO

Honey maturity is an important factor in evaluating the quality of honey; however, there are few studies on the molecular mechanism of the formation of mature honey (MH). In this study, the different metabolites of stomach honey (SH), immature honey (IMH), and MH samples during the same rapeseed flower season were analyzed by an UPLC-QTOF-MS-based metabolomics approach. MH was found to have a distinct metabolic profile to IMH, and a total of 9 different metabolites were obtained, 3 of which, including decenedioic acid, were accumulated in MH. Decenedioic acid as a bee-originated fatty acid (FA) was further verified in rape, acacia, and jujube honey by GC-MS, making it a potential marker to discriminate IMH and MH (P < 0.01). Besides, MH showed higher FAs concentrations; in particular, 3 FAs were significantly richer in MH than IMH (P < 0.05). This study reveals the molecular mechanism from IMH to MH from a FA perspective.


Assuntos
Mel , Animais , Abelhas , Cromatografia Líquida , Cromatografia Gasosa-Espectrometria de Massas , Mel/análise , Metabolômica , Espectrometria de Massas em Tandem
16.
Microbiol Res ; 246: 126710, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33556709

RESUMO

The bacterium Pantoea ananatis is associated with devastating plant diseases that cause serious economic losses. Strain DZ-12 was previously isolated from maize brown rot leaves in Hebei Province, China and its genome sequencing revealed that it belongs to P. ananatis. It contains a large, endogenous plasmid, pDZ-12. Different studies have shown that virulence determinants are frequently carried on plasmids. To determine whether pDZ-12 from P. ananatis has any effect on pathogenicity, the plasmid was eliminated by substituting its native replication genes with temperature-sensitive replication genes. The resulting temperature-sensitive plasmid could be cured by growing cells at high temperature (37℃). Loss of pDZ-12 from P. ananatis DZ-12 led to a decreased disease severity in maize plants suggesting that the endogenous plasmid is important for pathogenesis. Loss of pDZ-12 also affected the ability of the bacterium to form biofilms. The study provides the first evidence that the endogenous plasmid of P. ananatis DZ-12 is important for pathogenesis in maize plants and carries genes involved in biofilm formation. This study also presents the first report on curing a plasmid from P. ananatis.


Assuntos
Biofilmes/crescimento & desenvolvimento , Pantoea/genética , Doenças das Plantas/microbiologia , Plasmídeos/genética , Virulência , Zea mays/microbiologia , China , DNA Bacteriano , Pantoea/patogenicidade , Filogenia , Folhas de Planta/microbiologia , Replicon , Sequenciamento Completo do Genoma
17.
J Agric Food Chem ; 69(6): 1877-1887, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33543617

RESUMO

Bee pollen possesses potential hypoglycemic effects but its inhibitory mechanisms on glucose absorption and transportation in intestinal cells still need to be clarified. Here, we determined the inhibitory effects of bee pollen extract originating from Camellia sinensis L. (BP-Cs) as well as its representative phenolic compounds on glucose uptake and transport through a human intestinal Caco-2 cell monolayer model. It showed that three representative phenolic compounds, including gallic acid (GA), 3-O-[6'-O-(trans-p-coumaroyl)-ß-d-glucopyranosyl]kaempferol (K1), and 3-O-[2',6'-di-O-(trans-p-coumaroyl)-ß-d-glucopyranosyl]kaempferol (K2), with contents of 27.7 ± 0.86, 9.88 ± 0.54, and 7.83 ± 0.46 µg/mg in BP-Cs extract, respectively, exerted mutual antagonistic actions interacting with glucose transporters to inhibit glucose uptake and transport based on their combination index (CI) and molecular docking analysis. K1, K2, and GA might compete with d-glucose to form hydrogen bonds with the same active residues including GLU-412, GLY-416, GLN-314, and TRP-420 in GLUT2. These findings provide us a deep understanding of the mechanisms underlying the anti-hyperglycemia by bee pollen, which provide a new sight on dietary intervention strategies against diabetes.


Assuntos
Camellia sinensis , Animais , Abelhas , Células CACO-2 , Glucose , Proteínas Facilitadoras de Transporte de Glucose , Humanos , Simulação de Acoplamento Molecular , Extratos Vegetais/farmacologia , Pólen
18.
PLoS Biol ; 19(2): e3001122, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33630828

RESUMO

The Hippo-YAP pathway responds to diverse environmental cues to manage tissue homeostasis, organ regeneration, tumorigenesis, and immunity. However, how phosphatase(s) directly target Yes-associated protein (YAP) and determine its physiological activity are still inconclusive. Here, we utilized an unbiased phosphatome screening and identified protein phosphatase magnesium-dependent 1A (PPM1A/PP2Cα) as the bona fide and physiological YAP phosphatase. We found that PPM1A was associated with YAP/TAZ in both the cytoplasm and the nucleus to directly eliminate phospho-S127 on YAP, which conferring YAP the nuclear distribution and transcription potency. Accordingly, genetic ablation or depletion of PPM1A in cells, organoids, and mice elicited an enhanced YAP/TAZ cytoplasmic retention and resulted in the diminished cell proliferation, severe gut regeneration defects in colitis, and impeded liver regeneration upon injury. These regeneration defects in murine model were largely rescued via a genetic large tumor suppressor kinase 1 (LATS1) deficiency or the pharmacological inhibition of Hippo-YAP signaling. Therefore, we identify a physiological phosphatase of YAP/TAZ, describe its critical effects in YAP/TAZ cellular distribution, and demonstrate its physiological roles in mammalian organ regeneration.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteína Fosfatase 2C/metabolismo , Regeneração/fisiologia , Fatores de Transcrição/metabolismo , Animais , Proliferação de Células , Células Cultivadas , Colite/patologia , Humanos , Intestinos/fisiologia , Regeneração Hepática/fisiologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Organoides , Proteína Fosfatase 2C/genética , Transdução de Sinais
19.
Sensors (Basel) ; 21(2)2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33429893

RESUMO

Deep learning methods have been widely applied to visual and acoustic technology. In this paper, we propose an odor labeling convolutional encoder-decoder (OLCE) for odor identification in machine olfaction. OLCE composes a convolutional neural network encoder and decoder where the encoder output is constrained to odor labels. An electronic nose was used for the data collection of gas responses followed by a normative experimental procedure. Several evaluation indexes were calculated to evaluate the algorithm effectiveness: accuracy 92.57%, precision 92.29%, recall rate 92.06%, F1-Score 91.96%, and Kappa coefficient 90.76%. We also compared the model with some algorithms used in machine olfaction. The comparison result demonstrated that OLCE had the best performance among these algorithms.

20.
Cell Rep ; 34(1): 108580, 2021 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-33406439

RESUMO

Genetically identical female honeybee larvae with different diets develop into sterile workers or fertile queens. It remains unknown whether the reversible RNA N6-methyladenosine (m6A) mark functionally impact this "caste differentiation." Here, we profile the transcriptome-wide m6A methylome of honeybee queen and worker larvae at three instar stages and discover that m6A methylation dynamics are altered by differential feeding. Multiple methylome comparisons show an obvious increase in m6A marks during larval development and reveal a negative correlation between gene expression and m6A methylation. Notably, we find that worker larvae contain more hypermethylated m6A peaks than do queen larvae, and many caste-differentiation-related transcripts are differentially methylated. Chemical suppression of m6A methylation in worker larvae by 3-deazaadenosine (DAA) reduces overall m6A methylation levels and triggers worker larvae to develop queen caste features. Thus, our study demonstrates that m6A functionally impacts caste differentiation and larval development, yet it does not exclude potential contributions from other factors.

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