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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(11): 1099-1104, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31753092

RESUMO

OBJECTIVE: To investigate the current status of neglect in children aged 3-6 years in the rural areas of Urumqi, China and risk factors for neglect. METHODS: Stratified random cluster sampling was performed to select 1 326 children, aged 3-6 years, from 6 kindergartens in the rural areas of Urumqi. A self-designed questionnaire and "Chinese Neglect Evaluation Questionnaire for Rural Children Aged 3-6 Years" were used for investigation. RESULTS: Of the 1 326 children, the neglect rate was 51.89% (688 cases), and the degree of neglect was 49±6. There were significant differences in the neglect rate and the degree of neglect between the children in different age groups (P<0.01). The single-parent families and the remarried families had significantly higher child neglect rate and degree of neglect than the families of three generations under one roof and the core families (P<0.01). The children in left-behind status had a significantly higher degree of neglect (P<0.01). The multivariate logistic regression analysis showed that younger children, mothers with low education level, employment status of the mother as a migrant worker, single-parent and remarried families, low-income families, and left-behind status were risk factors for child neglect (P<0.05). CONCLUSIONS: The neglect of children aged 3-6 years in the rural areas of Urumqi is serious, and more attention should be paid to younger children, children whose mothers with low education level, left-behind children, and children from single-parent families, remarried families and low-income families.


Assuntos
Maus-Tratos Infantis , Criança , Pré-Escolar , China , Feminino , Humanos , Mães , Fatores de Risco , População Rural , Inquéritos e Questionários
2.
Eur Arch Otorhinolaryngol ; 276(9): 2565-2576, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31240455

RESUMO

PURPOSE: Nasopharyngeal carcinoma (NPC) is one of the most malignant head and neck carcinomas with unique epidemiological features. In this study, we aimed to identify the novel NPC-related genes and biological pathways, shedding light on the potential molecular mechanisms of NPC. METHODS: Based on Gene Expression Omnibus (GEO) database, an integrated analysis of microarrays studies was performed to identify differentially expressed genes (DEGs) and differentially methylated genes (DMGs) in NPC compared to normal control. The genes which were both differentially expressed and differentially methylated were identified. Functional annotation and protein-protein interaction (PPI) network construction were used to uncover biological functions of DEGs. RESULTS: Two DNA methylation and five gene expression datasets were incorporated. A total of 1074 genes were up-regulated and 939 genes were down-regulated in NPC were identified. A total of 719 differential methylation CpG sites (DMCs) including 1 hypermethylated sites and 718 hypomethylated sites were identified. Among which, 11 genes were both DEGs and DMGs in NPC. Pathways in cancer, p53 signaling pathway and Epstein-Barr virus infection were three pathways significantly enriched pathways in DEmRNAs of NPC. The PPI network of top 50 DEGs were consisted of 191 nodes and 191 edges. CONCLUSIONS: Our study was helpful to elucidate the underlying mechanism of NPC and provide clues for therapeutic methods.


Assuntos
Metilação de DNA/genética , Regulação Neoplásica da Expressão Gênica , Carcinoma Nasofaríngeo , Infecções por Vírus Epstein-Barr/genética , Redes Reguladoras de Genes/fisiologia , Estudo de Associação Genômica Ampla , Humanos , Carcinoma Nasofaríngeo/genética , Carcinoma Nasofaríngeo/patologia , Mapas de Interação de Proteínas , Transdução de Sinais
3.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(2): 184-188, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30782285

RESUMO

OBJECTIVE: To investigate the prevalence of social anxiety among the fourth-, fifth- and sixth-grade primary school students with myopia in Urumqi, China and the risk factors for social anxiety. METHODS: Stratified cluster random sampling was used to select 552 fourth-, fifth- and sixth-grade primary school students with myopia from four primary schools in Urumqi. A self-designed questionnaire, a social anxiety scale for children and a self-esteem scale were used to investigate the general demographic data and the current status of social anxiety and self-esteem. A multivariate logistic regression analysis was used to investigate the risk factors for social anxiety in primary school students with myopia. RESULTS: Of the 552 children, 173 (31.3%) were found to have social anxiety. The multivariate logistic regression analysis showed that a higher grade, female sex, autocratic family, high myopia, low self-esteem and wearing glasses for more than 2 years were risk factors for social anxiety (P<0.05). CONCLUSIONS: There is a serious problem of social anxiety among the fourth-, fifth- and sixth-grade primary school students with myopia in Urumqi. The development of social anxiety is associated with age, sex, degree of myopia, time of wearing glasses, parental education style and self-esteem level.


Assuntos
Ansiedade , Miopia , China , Feminino , Humanos , Fatores de Risco , Estudantes , Inquéritos e Questionários
4.
Bioorg Chem ; 95: 102927, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-31931286

RESUMO

Three new methylated Δ8-pregnene steroids, stemphylisteroids A-C (1-3) were isolated from the medicinal plant Polyalthia laui-derived fungus Stemphylium sp. AZGP4-2. Their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. The absolute configuration of 1 was determined by X-ray crystallographic analysis. Compound 1 show antibacterial activity against Escherichia coli with the MIC value of 6.25 µg/mL, and 2 exhibited a broad spectrum of antibacterial activities against six pathogenic bacteria with the MIC values ranging from 12.5 to 50 µg/mL. The discovery of three methylated Δ8-pregnene steroids 1-3 are a further addition to diverse and complex array of methylated steroids.

5.
Zhongguo Dang Dai Er Ke Za Zhi ; 20(12): 1044-1049, 2018 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-30572996

RESUMO

OBJECTIVE: To investigate the association between parental feeding behaviors and body mass index (BMI) of children, and to provide a scientific basis for the prevention and intervention of overweight and obesity in children. METHODS: Stratified random cluster sampling was used to select 976 children from 7 kindergartens in Xinshi District of Urumqi, and a questionnaire survey and physical measurement were performed to obtain related data. RESULTS: A total of 976 questionnaires were distributed and 924 (94.7%) usable questionnaires were collected. The overall detection rates of underweight, overweight, and obesity in children were 3.1% (29 children), 9.2% (85 children), and 6.7% (62 children), respectively. The highest level of parental feeding behavior was monitoring, followed by restriction and pressure to eat. The parents of the Han children had significantly higher levels of restriction and pressure to eat than those of the Uygur children (P<0.01). As for parental feeding behaviors, restriction to eat was positively correlated with BMI of the Han and Uygur boys (P<0.01); pressure to eat was negatively correlated with BMI of the Han boys and girls (P<0.01) and was positively correlated with BMI of the Uygur boys and girls (P<0.01); monitoring was negatively correlated with BMI of the Han and Uygur boys and girls (P<0.05). Among the parents of the Han and Uygur boys, the parents of the overweight/obese children had significantly higher scores of restriction to eat than those of the children with normal weight (P<0.05); among the parents of the Uygur boys and girls, the parents of the overweight/obese children had significantly higher scores of pressure to eat than those of the children with normal weight (P<0.01); among the parents of the Uygur and Han boys and girls, the parents of the overweight/obese children had significantly lower scores of monitoring than those of the children with normal weight (P<0.01). CONCLUSIONS: The parental feeding behavior is good in Urumqi, and the parental feeding behavior of the Uygur children is a little better than that of the Han children. Parental feeding behavior is closely correlated with BMI of children, and such correlation differs across ethnic groups and sexes. A high level of monitoring and low levels of restriction and pressure to eat may help to prevent and control the development of overweight and obesity in children.


Assuntos
Comportamento Alimentar , Índice de Massa Corporal , Peso Corporal , Criança , China , Feminino , Humanos , Masculino , Sobrepeso , Pais , Inquéritos e Questionários
6.
Onco Targets Ther ; 11: 7635-7642, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30464512

RESUMO

Background: (PD-L2), a ligand of programmed cell death protein 1 (PD-1), is an inhibitory receptor of T cells and activated B cells. Many studies have focused on PD-L1, another ligand of PD-1, and the prognostic significance of PD-L1 has been reported in many tumors. However, the expression of PD-L2 in relation to clinical outcomes has not been fully investigated in cancer patients. Patients and methods: In this study, we investigated the expression of PD-L2 via immunohistochemistry (IHC) in the pathological specimens of 348 patients treated for colorectal cancer (CRC). Results: Strong PD-L2 expression was found in the cancer tissues from 41% of the CRC patients who also had a high TNM stage and carcinoembryonic antigen (CEA) concentration. We also carried out functional studies in vitro, which showed that PD-L2 did not influence the growth of the CRC cell line HCT116, but increased cell invasion. Conclusion: Collectively, these findings suggest that PD-L2 may be a potential therapeutic target for CRC.

7.
J Biomed Res ; 32(5): 336-342, 2018 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-30249816

RESUMO

While obesity and fat intake have been associated with the risk and prognosis of epithelial ovarian cancer, the association between the lipid levels and epithelial ovarian cancer phenotype remains controversial. We conducted a retrospective study of 349 epithelial ovarian cancer patients who received treatment at Jiangsu Cancer Hospital, China between 2011 and 2017. We analyzed age at diagnosis, blood pressure, plasma glucose content, body mass index (BMI), lipid levels and clinical parameters. Severity of epithelial ovarian cancer was classified according to the International Federation of Gynecology and Obstetrics (FIGO) grading system. Univariate analysis of the clinical factors according to the severity of epithelial ovarian cancer was followed by logistic regression analysis to identify clinical factors significantly associated with epithelial ovarian cancer severity. Univariate analysis indicated that age, BMI, triglyceride (TG), and high density lipoproteins (HDL) differed significantly among different stages of epithelial ovarian cancer (P<0.05). In the logistic regression model, elevated TG (OR: 1.883; 95% CI= 1.207-2.937), and low HDL (OR: 0.497; 95% CI= 0.298-0.829) levels were significantly associated with the high severity epithelial ovarian cancer. Our data indicate that high TG and low HDL levels correlate with a high severity of epithelial ovarian cancer. These data provide important insight into the potential relationship between the lipid pathway and epithelial ovarian cancer phenotype and development.

8.
Zhongguo Dang Dai Er Ke Za Zhi ; 20(8): 670-674, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30111478

RESUMO

OBJECTIVE: To investigate the current status of social anxiety and depression among the fourth-, fifth-, and sixth-grade students in Urumqi, China, and to provide data support for mental health education for primary school students in the future. METHODS: Stratified cluster random sampling was performed to select 919 fourth-, fifth-, and sixth-grade students from four primary schools for the Han and minority ethnic groups in Urumqi as research subjects. Social Anxiety Scale for Children and Children's Depression Inventory were used to evaluate the current status of social anxiety and depression. RESULTS: Among the 919 fourth-, fifth-, and sixth-grade students, the detection rate of social anxiety was 28.5% (262 students). The fourth-grade students had significantly lower scores on each subscale of social anxiety and total score of social anxiety than the fifth- and sixth-grade students (P<0.05). Girls had significantly higher score on the subscale of fear of negative evaluation and total score of social anxiety than boys (P<0.05). The Uyghur students had significantly higher scores on each subscale of social anxiety and total score of social anxiety than the Han students and the students of other minority ethnic groups (P<0.05). Among these fourth-, fifth-, and sixth-grade students, the detection rate of depression was 11.2% (103 students). The fourth-grade students had significantly lower scores on the subscales of negative self-esteem, ineffectiveness, and interpersonal problems and total score of depression than the fifth- and sixth-grade students (P<0.05). The Uyghur students had significantly higher scores on the subscales of anhedonia and negative mood and total score of depression than the Han students and the students of other minority ethnic groups (P<0.05). CONCLUSIONS: There are significant differences in the status of social anxiety and depression among the primary school students with different grades, sexes or ethnic groups in Urumqi. The fifth- and sixth-grade students, girls, and Uyghur students tend to have more serious psychological problems, which should be taken seriously by parents, schools, and the society.


Assuntos
Ansiedade/epidemiologia , Depressão/epidemiologia , Adolescente , Ansiedade/etnologia , Criança , China/epidemiologia , China/etnologia , Depressão/etnologia , Medo , Feminino , Humanos , Masculino , Instituições Acadêmicas/estatística & dados numéricos , Autoimagem , Comportamento Social , Estudantes/psicologia , Estudantes/estatística & dados numéricos
9.
Oncol Lett ; 15(5): 7716-7724, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29731901

RESUMO

Tetrandrine (TET) exhibits biological activities, including anticancer activity. In Chinese medicine, TET has been used to treat hypertensive and arrhythmic conditions and has been demonstrated to induce cytotoxic effects on human cancer cell lines. However, to the best of the author's knowledge, no previous studies have revealed that TET affects cell metastasis in SW620 human colon cancer cells. The present study demonstrated that TET decreased the cell number and inhibited cell adhesion and mobility of SW620 cells. Furthermore, a wound healing assay was performed to demonstrate that TET suppressed cell movement, and Transwell chamber assays were used to reveal that TET suppressed the cell migration and invasion of SW620 cells. Western blotting demonstrated that TET significantly reduced protein expression levels of SOS Ras/Rac guanine nucleotide exchange factor 1, phosphatidylinositol 3-kinase, growth factor receptor bound protein 2, phosphorylated (p)-c Jun N-terminal kinase 1/2, p-p38, p38, 14-3-3, Rho A, ß-catenin, nuclear factor-κB p65, signal transducer and activator of transcription-1 and cyclooxygenase-2, in comparison with untreated SW620 cells. Overall, the results of the present study suggested that TET may be used as a novel anti-metastasis agent for the treatment of human colon cancer in the future.

10.
Sci Rep ; 7(1): 8430, 2017 08 16.
Artigo em Inglês | MEDLINE | ID: mdl-28814728

RESUMO

Few studies have compared the prevalence of smoking between patients with bipolar disorder, major depressive disorder (MDD) and schizophrenia. This study examined the prevalence of smoking and its relationships with demographic and clinical characteristics, and quality of life (QOL) in patients with these psychiatric disorders. A total of 1,102 inpatients were consecutively screened. Psychopathology and QOL were measured with standardized instruments. The prevalence of current smoking in the whole sample was 16.7%; 17.5% in bipolar disorder, 10.6% in MDD and 18.5% in schizophrenia. The rates of smoking in bipolar disorder (p = 0.004, OR = 2.5, 95%CI: 1.3-4.7) and schizophrenia (p = 0.03, OR = 2.0, 95%CI: 1.06-3.8) were significantly higher than in MDD, while no difference was found between bipolar disorder and schizophrenia. Smokers had a higher mental QOL than non-smokers (p = 0.007) in MDD, but no difference was found in the other two groups. Male gender, living alone, higher personal income, older age of onset, health insurance coverage, and first episode was significantly associated with smoking in one or more diagnostic groups. Smoking appears more common in bipolar disorder and schizophrenia than in MDD in China. The figures in all disorders were lower than that reported in most of other countries.


Assuntos
Transtorno Bipolar/psicologia , Transtorno Depressivo Maior/psicologia , Qualidade de Vida , Fumar/epidemiologia , Adulto , Transtorno Bipolar/epidemiologia , China/epidemiologia , Transtorno Depressivo Maior/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esquizofrenia/epidemiologia , Psicologia do Esquizofrênico
11.
Psychiatry Res ; 246: 246-249, 2016 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-27723522

RESUMO

Little is known about the pattern of electroconvulsive therapy (ECT) use in the clinical population in China. This study examined the percentage of ECT use and its association with clinical characteristics and quality of life (QOL) in a psychiatric center in China that caters for a population of 20 million. A total sample of 1364 inpatients was consecutively recruited for the study. Demographic and clinical data including the use of ECT were collected. Psychopathology, activity of daily living and QOL were measured using standardized instruments. The percentage of ECT use was 52.1% in the whole sample; 53.4% in major depression, 57.8% in bipolar disorder, 57.0% in schizophrenia and 32.4% in other diagnoses. There was no significant difference between the ECT and non-ECT groups in any domain of QOL. Multivariate analyses revealed that ECT was independently associated with the diagnoses of major depression, bipolar disorder and schizophrenia, physical restraint, severe aggression, better activity of daily living skills, more frequent use of antipsychotics and less frequent use of benzodiazepines. The percentage of ECT use was much greater in a major psychiatric center in China than those reported from other parts of the world. Use of ECT had no influence on the short-term QOL. Further investigations are warranted to explore the reasons for the high percentage of ECT use.


Assuntos
Transtorno Bipolar/terapia , Transtorno Depressivo Maior/terapia , Eletroconvulsoterapia/estatística & dados numéricos , Hospitais Psiquiátricos/estatística & dados numéricos , Transtornos Mentais/terapia , Qualidade de Vida , Esquizofrenia/terapia , Adulto , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
12.
Am J Chin Med ; 44(6): 1289-1310, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27627923

RESUMO

Sulforaphane (SFN), an isothiocyanate, exists exclusively in cruciferous vegetables, and has been shown to possess potent antitumor and chemopreventive activity. However, there is no available information that shows SFN affecting human colon cancer HCT 116 cells. In the present study, we found that SFN induced cell morphological changes, which were photographed by contrast-phase microscopy, and decreased viability. SFN also induced G2/M phase arrest and cell apoptosis in HCT 116 cells, which were measured with flow cytometric assays. Western blotting indicated that SFN increased Cyclin A, cdk 2, Cyclin B and WEE1, but decreased Cdc 25C, cdk1 protein expressions that led to G2/M phase arrest. Apoptotic cell death was also confirmed by Annexin V/PI and DAPI staining and DNA gel electrophoresis in HCT 116 cells after exposure to SFN. The flow cytometric assay also showed that SFN induced the generation of reactive oxygen species (ROS) and Ca[Formula: see text] and decreased mitochondria membrane potential and increased caspase-8, -9 and -3 activities in HCT 116 cell. Western blotting also showed that SFN induced the release of cytochrome c, and AIF, which was confirmed by confocal microscopy examination. SFN induced ER stress-associated protein expression. Based on those observations, we suggest that SFN may be used as a novel anticancer agent for the treatment of human colon cancer in the future.


Assuntos
Antineoplásicos Fitogênicos , Apoptose/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Neoplasias do Colo/patologia , Fase G2/efeitos dos fármacos , Isotiocianatos/química , Fitoterapia , Extratos Vegetais/farmacologia , Anexina A5/genética , Anexina A5/metabolismo , Apoptose/genética , Cálcio/metabolismo , Caspases/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Morte Celular/genética , Divisão Celular/genética , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/metabolismo , Estresse do Retículo Endoplasmático/genética , Fase G2/genética , Expressão Gênica/efeitos dos fármacos , Células HCT116 , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Espécies Reativas de Oxigênio/metabolismo , Estimulação Química
13.
Compr Psychiatry ; 71: 71-76, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27639124

RESUMO

PURPOSE: To examine the frequency of hyperprolactinemia and the socio-demographic, clinical, and quality of life (QOL) correlates. The frequency of prolactin-related side effects and associated subjective experiences were also examined. METHODS: A cohort of 1364 psychiatric inpatients were consecutively recruited and evaluated. Basic socio-demographic and clinical data were collected. Psychopathology, prolactin-related side effects were measured using standardized instruments. QOL was assessed using the Medical Outcomes Study Short Form 12. RESULTS: The frequency of hyperprolactinemia was 61.3% in the whole sample; 61.6% in female and 60.8% in male patients. There was no significant association between hyperprolactinemia and any QOL domain. In the whole sample, 15.1% of patients reported moderately severe breast symptoms and lactation, and 53.9% reported moderate or severe discomfort. Nearly a third of female patients (30.4%) reported at least moderate menstrual changes and 50.2% moderate or severe discomfort, while 24.2% of male patients reported at least moderate erectile dysfunction and 52.6% moderate or severe discomfort. Multiple logistic regression analysis revealed that patients with hyperprolactinemia were less likely to be married, diagnosed with mood disorders, or treated with clozapine, aripiprazole, or antidepressants but more likely to receive risperidone. CONCLUSIONS: Effective measures to lower the frequency of hyperprolactinemia and the related side effects should be considered in Chinese psychiatric facilities.


Assuntos
Hiperprolactinemia/epidemiologia , Transtornos Mentais/epidemiologia , Qualidade de Vida , Disfunções Sexuais Fisiológicas/epidemiologia , Adulto , Antidepressivos/uso terapêutico , Antipsicóticos/uso terapêutico , Aripiprazol/uso terapêutico , Estudos de Casos e Controles , China/epidemiologia , Clozapina/uso terapêutico , Feminino , Humanos , Masculino , Transtornos Mentais/tratamento farmacológico , Risperidona/uso terapêutico , Disfunções Sexuais Fisiológicas/induzido quimicamente , Adulto Jovem
14.
Molecules ; 21(7)2016 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-27376261

RESUMO

In this study we investigate the molecular mechanisms of caspases and mitochondria in the extrinsic and intrinsic signal apoptosis pathways in human leukemia HL-60 cells after in vitro exposure to 18α-glycyrrhetinic acid (18α-GA). Cells were exposed to 18α-GA at various concentrations for various time periods and were harvested for flow cytometry total viable cell and apoptotic cell death measurements. Cells treated with 18α-GA significantly inhibited cell proliferation and induced cell apoptosis in a dose-dependent manner, with an IC50 value of 100 µM at 48 h. The cell growth inhibition resulted in induction of apoptosis and decreased the mitochondria membrane potential (ΔΨm) and increased caspase-8, -9 and -3 activities. Furthermore, cytochrome c and AIF were released from mitochondria, as shown by western blotting and confirmed by confocal laser microscopy. Western blotting showed that 18α-GA increased the levels of pro-apoptotic proteins such as Bax and Bid and decreased the anti-apoptotic proteins such as Bcl-2 and Bcl-xl, furthermore, results also showed that 18α-GA increased Fas and Fas-L which are associated with surface death receptor in HL-60 cells. Based on those observations, the present study supports the hypothesis that 18α-GA-induced apoptosis in HL-60 cells involves the activation of the both extrinsic and intrinsic apoptotic pathways.


Assuntos
Antineoplásicos/farmacologia , Caspases/metabolismo , Ácido Glicirretínico/análogos & derivados , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Transdução de Sinais/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/metabolismo , Proliferação de Células/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Expressão Gênica , Ácido Glicirretínico/farmacologia , Células HL-60 , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Transporte Proteico
15.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 24(2): 590-5, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-27151035

RESUMO

OBJECTIVE: To study the regulation of SIRT1 by transcription factor SREBP-1 in adipogeneic differentiation of bone marrow mesenchymal stem cells (BMMSC). METHODS: Oil red O staining was used to identify the adipogenic differentiation of BMMSC; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 gene were detected by RT-PCR; the expession level of SREBP-1 was determined by Western-blot. The chromatin immunoprecipitation (ChIP) assay was used to investigate the binding of SREBP-1 to SIRT1 promoter. RESULTS: BMMSC exposed to adipogenesis inducing medium become mature adipocytes at day 14; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 genes were up-regulated in adipocyte differentiation of BMMSC; the protein level of SREBP-1 was higher obviously; SIRT1 gene sequences was succesfully amplified from the genomic DNA immunoprecipitated by SREBP-1 antibody. CONCLUSION: SREBP-1 can bind to the promoter region of the SIRT1 gene in adipogenesis of BMMSC, and may be involved in the transcriptional regulation of the SIRT1 gene.


Assuntos
Adipócitos/citologia , Diferenciação Celular , Células-Tronco Mesenquimais/citologia , Sirtuína 1/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo , Adipogenia , Células Cultivadas , Imunoprecipitação da Cromatina , Regulação da Expressão Gênica , Humanos , Regiões Promotoras Genéticas , Regulação para Cima
16.
Psychiatry Res ; 241: 154-8, 2016 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-27179180

RESUMO

Physical restraint (PR) for patients is an ongoing controversial topic in psychiatry. This study examined the percentage of PR and its associations with clinical characteristics and the implementation of the National Mental Health Law (NMHL) in China. The study consecutively assessed a sample of 1364 psychiatric inpatients. Socio-demographic and clinical data including use of PR were collected from the medical records using a form designed for this study and confirmed via interview. Psychopathology and insight were measured using standardized instruments. The percentage of PR was 27.2% in the whole sample with 30.7% and 22.4% occurring respectively before and after the NMHL implementation (p=0.001). In multiple logistic regression analysis PR was positively associated with unemployment, lower income, aggression in the past month, being admitted before the NMHL implementation and poorer insight. The percentage of PR in Chinese psychiatric patients is associated with various clinical factors and appeared to decrease after the implementation of the NMHL. Focused and individualized care for patients who are unemployed, have low income, recent aggression and poor insight would be necessary at early stages of admission.


Assuntos
Hospitais Psiquiátricos/estatística & dados numéricos , Legislação Médica/estatística & dados numéricos , Transtornos Mentais/terapia , Pessoas Mentalmente Doentes/estatística & dados numéricos , Restrição Física/estatística & dados numéricos , Adulto , China , Feminino , Hospitais Psiquiátricos/legislação & jurisprudência , Humanos , Masculino , Pessoas Mentalmente Doentes/legislação & jurisprudência , Pessoa de Meia-Idade , Restrição Física/legislação & jurisprudência
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 24(1): 184-90, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-26913418

RESUMO

OBJECTIVE: To study the effects of LIF combined with bFGF on the proliferation, stemness and senescence of hUC-MSC. METHODS: Experiments were divided into 4 groups: control group, in which the cells were treated with complete medium (α-MEM containing 10% FBS); group LIF, in which the cells were treated with complete medium containing 10 ng/ml LIF; group bFGF, in which the cells were treated with complete medium containing 10 ng/ml bFGF; combination group, in which the cells were treated with complete medium containing 10 ng/ml LIF and 10 ng/ml bFGF. The growth curves of hUC-MSC at passage 4 in different groups were assayed by cell counting kit 8. Cellular morphologic changes were observed under inverted phase contrast microscope; hUC-MSC senescence in different groups was detected by ß-galactosidase staining. The expression of PCNA, P16, P21, P53, OCT4 and NANOG genes was detected by RT-PCR. RESULTS: The cell growth curves of each group were similar to the S-shape; the cell proliferation rate from high to low as follows: that in the combination group > group bFGF > group LIF > control group. Senescence and declining of proliferation were observed at hUC-MSC very early in control group; the cells in group LIF maintained good cellular morphology at early stage, but cell proliferation was slow and late senescence was observed; a few cells in group bFGF presented signs of senescence, but with quick proliferation; the cells in combination group grew quickly and maintained cellular morphology of hUC-MSC for long time. The LIF and bFGF up-regulated the expression of PCNA, OCT4 and NANOG, while they down-regulated the expression of P16, P21, P53, and their combinative effects were more significant. CONCLUSION: LIF combined with bFGF not only can promote the proliferation and maintenance of stemness of hUC-MSC, but also can delay the senescence of hUC-MSC.


Assuntos
Fator 2 de Crescimento de Fibroblastos/farmacologia , Fator Inibidor de Leucemia/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Ciclo Celular , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Genes Homeobox , Humanos , Células-Tronco Mesenquimais/citologia , Fator 3 de Transcrição de Octâmero/metabolismo , Compostos Orgânicos , Antígeno Nuclear de Célula em Proliferação/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Cordão Umbilical/citologia
18.
Environ Toxicol ; 31(11): 1640-1651, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26174008

RESUMO

Although reports have shown that α-phellandrene (α-PA) is one of the monoterpenes and is often used in the food and perfume industry, our previous studies have indicated that α-PA promoted immune responses in normal mice in vivo. However, there is no available information to show that α-PA induced cell apoptosis in cancer cells, thus, we investigated the effects of α-PA on the cell morphology, viability, cell cycle distribution, and apoptosis in mice leukemia WEHI-3 cells in vitro. Results indicated that α-PA induced cell morphological changes and decreased viability, induced G0/G1 arrest and sub-G1 phase (apoptosis) in WEHI-3 cells. α-PA increased the productions of reactive oxygen species (ROS) and Ca2+ and decreased the levels of mitochondrial membrane potential (ΔΨm ) in dose- and time-dependent manners in WEHI-3 cells that were analyzed by flow cytometer. Results from confocal laser microscopic system examinations show that α-PA promoted the release of cytochrome c, AIF, and Endo G from mitochondria in WEHI-3 cells. These results are the first findings to provide new information for understanding the mechanisms by which α-PA induces cell cycle arrest and apoptosis in WEHI-3 cells in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1640-1651, 2016.


Assuntos
Apoptose/efeitos dos fármacos , Monoterpenos/farmacologia , Animais , Caspases/metabolismo , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Leucemia/tratamento farmacológico , Leucemia/patologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Camundongos , Espécies Reativas de Oxigênio/metabolismo
19.
Am J Chin Med ; 43(6): 1247-64, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26446205

RESUMO

Bufalin, a component of Chan Su (a traditional Chinese medicine), has been known to have antitumor effects for thousands of years. In this study, we investigated its anti-metastasis effects on NCI-H460 lung cancer cells. Under sub-lethal concentrations (from 25 up to 100 nM), bufalin significantly inhibits the invasion and migration nature of NCI-H460 cells that were measured by Matrigel Cell Migration Assay and Invasion System. Bufalin also suppressed the enzymatic activity of matrix metalloproteinase (MMP)-9, which was examined by gelatin zymography methods. Western blotting revealed that bufalin depressed several key metastasis-related proteins, such as NF-κB, MMP-2, MMP-9, protein kinase C (PKC), phosphatidylinositol 3-kinase (PI3-K), phosphorylated Akt, growth factor receptor-bound protein 2 (GRB2), phosphorylated extracellular signal-regulated kinase (ERK), phosphorylated p38, and phosphorylated c-Jun NH2-terminal kinase (JNK). As evidenced by immunostaining and the electrophoretic mobility shift assay (EMSA), bufalin induced not only a decreased cytoplasmic NF-κB production, but also decreased its nuclear translocation. Several metastasis-related genes, including Rho-associated (Rho A), coiled-coil-containing protein kinase 1 (ROCK1), and focal adhesion kinase (FAK), were down-regulated after bufalin treatment. In conclusion, bufalin is effective in inhibiting the metastatic nature of NCI-H460 cells in low, sub-lethal concentrations. Such an effect involves many mechanisms including MMPs, mitogen-activated protein kinases (MAPKs) and NF-κB systems. Bufalin has a potential to evolve into an anti-metastasis drug for human lung cancer in the future.


Assuntos
Bufanolídeos/farmacologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Neoplasias Pulmonares/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , NF-kappa B/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/genética , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/enzimologia , Neoplasias Pulmonares/genética , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 9 da Matriz/genética , NF-kappa B/genética , Invasividade Neoplásica , Metástase Neoplásica , Transdução de Sinais/efeitos dos fármacos
20.
J BUON ; 20(3): 918-22, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26214647

RESUMO

PURPOSE: A meta-analysis was performed to determine the association between MTRR A66G polymorphism and colorectal cancer (CRC) susceptibility. METHODS: Based on comprehensive searches of the MEDLINE, EMBASE and ISI Web of knowledge, China National Knowledge Infrastructure (CNKI) and Wanfang Database, we identified eligible studies about the association between MTRR A66G polymorphism and CRC susceptibility. RESULTS: A total of 6020 cases and 8317 controls in 15 studies were pooled together for evaluation of the overall association between MTRR A66G polymorphism and susceptibility of CRC. The allele model (G vs A: p=0.01; OR=1.07, 95% CI=1.02-1.12), and homozygous model (GG vs AA: p=0.006; OR=1.15, 95% CI=1.04-1.28) showed increased risk for CRC development. Similarly, the dominant model (GG+GA vs AA: p=0.04; OR=1.11, 95% CI=1.01-1.22) and the recessive model (GG vs GA+AA: p=0.04; OR=1.08, 95% CI=1.00-1.17) showed increased risk for CRC development. In the analysis stratified by ethnicity (Caucasian and East Asian), significant associations were found between MTRR A66G polymorphism and susceptibility to CRC among Caucasians. CONCLUSION: Our pooled data suggest an association between MTRR A66G polymorphism and CRC susceptibility among Caucasians.


Assuntos
Neoplasias Colorretais/genética , Ferredoxina-NADP Redutase/genética , Polimorfismo Genético , Estudos de Casos e Controles , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/enzimologia , Neoplasias Colorretais/etnologia , Grupo com Ancestrais do Continente Europeu/genética , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Razão de Chances , Fenótipo , Medição de Risco , Fatores de Risco
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