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1.
Nat Commun ; 12(1): 5072, 2021 08 20.
Artigo em Inglês | MEDLINE | ID: mdl-34417473

RESUMO

In vivo bioprinting has recently emerged as a direct fabrication technique to create artificial tissues and medical devices on target sites within the body, enabling advanced clinical strategies. However, existing in vivo bioprinting methods are often limited to applications near the skin or require open surgery for printing on internal organs. Here, we report a ferromagnetic soft catheter robot (FSCR) system capable of in situ computer-controlled bioprinting in a minimally invasive manner based on magnetic actuation. The FSCR is designed by dispersing ferromagnetic particles in a fiber-reinforced polymer matrix. This design results in stable ink extrusion and allows for printing various materials with different rheological properties and functionalities. A superimposed magnetic field drives the FSCR to achieve digitally controlled printing with high accuracy. We demonstrate printing multiple patterns on planar surfaces, and considering the non-planar surface of natural organs, we then develop an in situ printing strategy for curved surfaces and demonstrate minimally invasive in vivo bioprinting of hydrogels in a rat model. Our catheter robot will permit intelligent and minimally invasive bio-fabrication.


Assuntos
Bioimpressão , Cateteres , Imãs/química , Robótica , Animais , Linhagem Celular , Elasticidade , Condutividade Elétrica , Humanos , Hidrogéis/química , Fígado/diagnóstico por imagem , Ratos Sprague-Dawley , Suínos , Tomografia Computadorizada por Raios X , Viscosidade
2.
BMC Genomics ; 21(1): 559, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32795331

RESUMO

BACKGROUND: Mud crab, Scylla paramamosain, a euryhaline crustacean species, mainly inhabits the Indo-Western Pacific region. Wild mud crab spawn in high-salt condition and the salinity reduced with the growth of the hatching larvae. When the larvae grow up to megalopa, they migrate back to estuaries and coasts in virtue of the flood tide, settle and recruit adult habitats and metamorphose into the crablet stage. Adult crab can even survive in a wide salinity of 0-35 ppt. To investigate the mRNA profile after salinity stress, S. paramamosain megalopa were exposed to different salinity seawater (low, 14 ppt; control, 25 ppt; high, 39 ppt). RESULTS: Firstly, from the expression profiles of Na+/K+/2Cl- cotransporter, chloride channel protein 2, and ABC transporter, it turned out that the 24 h might be the most influenced duration in the short-term stress. We collected megalopa under different salinity for 24 h and then submitted to mRNA profiling. Totally, 57.87 Gb Clean Data were obtained. The comparative genomic analysis detected 342 differentially expressed genes (DEGs). The most significantly DEGs include gamma-butyrobetaine dioxygenase-like, facilitated trehalose transporter Tret1, sodium/potassium-transporting ATPase subunit alpha, rhodanese 1-like protein, etc. And the significantly enriched pathways were lysine degradation, choline metabolism in cancer, phospholipase D signaling pathway, Fc gamma R-mediated phagocytosis, and sphingolipid signaling pathway. The results indicate that in the short-term salinity stress, the megalopa might regulate some mechanism such as metabolism, immunity responses, osmoregulation to adapt to the alteration of the environment. CONCLUSIONS: This study represents the first genome-wide transcriptome analysis of S. paramamosain megalopa for studying its stress adaption mechanisms under different salinity. The results reveal numbers of genes modified by salinity stress and some important pathways, which will provide valuable resources for discovering the molecular basis of salinity stress adaptation of S. paramamosain larvae and further boost the understanding of the potential molecular mechanisms of salinity stress adaptation for crustacean species.


Assuntos
Braquiúros , Adaptação Fisiológica/genética , Animais , Braquiúros/genética , Perfilação da Expressão Gênica , RNA Mensageiro/genética , Salinidade , Estresse Salino
3.
Food Sci Nutr ; 8(1): 104-113, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31993137

RESUMO

Aroma is an important index of tea quality. The volatile C6-compounds formed from linoleic and linolenic acids in tea leaf lipids are essential components of tea. C6-compounds are formed and transformed during the postharvest process of tea leaves. However, the metabolic flux of these C6-compounds, the activities of related enzymes, and the transcription of related genes during the postharvest process of oolong tea remain unclear. In this study, the chemical profiles of C6-aldehydes and C6-alcohols, the pattern of ADH enzyme activity, and the level of CsADH gene expression during the postharvest process of oolong tea were investigated. We found that the turnover process had a positive effect on the accumulation of C6-alcohols and simultaneously induced ADH activity, especially during the withering stage. The expression of CsADH peaked during the turnover stage. The relative expression level of CSA019598 typically increased during the postharvest process. Correlation analysis demonstrated that CSA019598 expression increased as ADH activity increased. This finding suggests that CSA019598 may play a prominent role in regulating ADH. These results advance our understanding of C6-compound formation during the postharvest process of oolong tea. We aim to evaluate how green leaf volatiles affect the enzymatic formation and genetic transcription of aromatic compounds in oolong tea in future studies.

4.
Opt Express ; 27(24): 35884-35894, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31878753

RESUMO

Multi-line Structured Light Projection System (MSLPS) is widely used with the advantages of high-contrast stripes and high robustness in high-speed dynamic reconstruction in comparison with Phase Modulation System (PMS). However, existing calibration methods are either sophisticated or low-accuracy in large visual field application because the bending of line structured light plane caused by lens distortion is inevitable and projector calibration is necessary. Therefore, in this paper, we present an accurate and robust calibration method based on ray-tracing, which establishes accurate relationship between subpixel coordinates of each bending line structured light plane and 3D coordinates based on camera coordinate system without projector calibration. As long as placing a checkerboard at random positions, MSLPS can be calibrated and hence is more suitable in practical application. Experimental results demonstrated that, compared with existing line structured light strategies, the proposed method could achieve more accurate and robust 3D reconstruction and the calibration process is simpler and easier to implement.

5.
PeerJ ; 7: e6385, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30723635

RESUMO

Tea is one of three major non-alcoholic beverages that are popular all around the world. The economic value of tea product largely depends on the post-harvest physiology of tea leaves. The utilization of quantitative reverse transcription polymerase chain reaction is a widely accepted and precise approach to determine the target gene expression of tea plants, and the reliability of results hinges on the selection of suitable reference genes. A few reliable reference genes have been documented using various treatments and different tissues of tea plants, but none has been done on post-harvest leaves during the tea manufacturing process. The present study selected and analyzed 15 candidate reference genes: Cs18SrRNA, CsGADPH, CsACT, CsEF-1α, CsUbi, CsTUA, Cs26SrRNA, CsRuBP, CsCYP, CselF-4α, CsMON1, CsPCS1, CsSAND, CsPPA2, CsTBP. This study made an assessment on the expression stability under two kinds of post-harvest treatment, turn over and withering, using three algorithms-GeNorm, Normfinder, and Bestkeeper. The results indicated that the three commonly used reference genes, CsTUA, Cs18SrRNA, CsRuBP, together with Cs26SrRNA, were the most unstable genes in both the turn over and withering treatments. CsACT, CsEF-1α, CsPPA2, and CsTBP were the top four reference genes in the turn over treatment, while CsTBP, CsPCS1, CsPPA2, CselF-4α, and CsACT were the five best reference genes in the withering group. The expression level of lipoxygenase genes, which were involved in a number of diverse aspects of plant physiology, including wounding, was evaluated to validate the findings. To conclude, we found a basis for the selection of reference genes for accurate transcription normalization in post-harvest leaves of tea plants.

6.
Mol Biol Rep ; 45(6): 1913-1918, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30203240

RESUMO

The blue swimming crab (Portunus pelagicus) is a valuable marine fishery resource in Indo-West Pacific Ocean. So far, rare genetic resource of this species is available. In this report, the restriction-site associated DNA (RAD) approach was employed to mine the genomic information and identify molecular markers in P. pelagicus. A total of 0.82 Gbp clean data were generated from the genome of individual "X2A". De novo assembly produced 85,796 contigs with an average length of 339 bp. A total of 45,464 putative SNPs and 17,983 microsatellite loci were identified from the genomes of ten individuals. Furthermore, 31 pairs of primers were successfully designed, with 16 of them exhibiting polymorphism in a wild population. For these polymorphic loci, the expected and observed alleles per locus ranged from 1.064 to 7.314 and from 2 to 11, respectively. The expected and observed heterozygosity per locus ranged from 0.0615 to 0.819 and from 0.0626 to 1.000, respectively. Nine loci showed high informative with polymorphism information content (PIC) > 0.5. Five loci significantly deviated from Hardy-Weinberg equilibrium in the samples analyzed. No linkage disequilibrium was found among the 16 polymorphic microsatellite loci. This study provided massive genetic resource and polymorphic molecular markers that should be helpful for studies on conservation genetics, population dynamics and genetic diversity of P. pelagicus and related crab species.


Assuntos
Braquiúros/genética , Mapeamento por Restrição/métodos , Alelos , Animais , DNA , Frequência do Gene/genética , Loci Gênicos/genética , Variação Genética , Genética Populacional/métodos , Desequilíbrio de Ligação/genética , Repetições de Microssatélites/genética , Oceano Pacífico , Polimorfismo Genético/genética , Polimorfismo de Nucleotídeo Único/genética , Análise de Sequência de DNA/métodos
7.
Sci Data ; 5: 180131, 2018 07 10.
Artigo em Inglês | MEDLINE | ID: mdl-29989595

RESUMO

Crab culture has gained prominence in the last decade due to the large global market demand for live crabs and crab products. Portunus sanguinolentus is one of the economically important crab species in the Indo-Pacific region, with distinct differences in growth and size between male and female crabs, thus, leading to huge difference in their market values. The culture of P. sanguinolentus is still in its infancy, with crab supplies heavily dependent on wild catch. In order to unravel the molecular differences between male and female crabs, we generated a comprehensive transcriptomic dataset for P. sanguinolentus by sequencing the gonads of both sexes using the Illumina Hiseq 2500 system. Transcriptomes were assembled using Trinity de novo assembly followed by annotation. This transcriptomic data set for P. sanguinolentus would serve as an important reference data for genomic and genetic studies in this crab and related species.


Assuntos
Braquiúros/genética , Gônadas , Transcriptoma , Animais , Biomarcadores , Feminino , Genômica , Masculino
8.
Mar Biotechnol (NY) ; 20(1): 20-34, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29152671

RESUMO

The molecular mechanism underlying sex determination and gonadal differentiation of the mud crab (Scylla paramamosain) has received considerable attention, due to the remarkably biological and economic differences between sexes. However, sex-biased genes, especially non-coding genes, which account for these differences, remain elusive in this crustacean species. In this study, the first de novo gonad transcriptome sequencing was performed to identify both differentially expressed genes and long non-coding RNAs (lncRNAs) between male and female S. paramamosain by using Illumina Hiseq2500. A total of 79,282,758 and 79,854,234 reads were generated from ovarian and testicular cDNA libraries, respectively. After filtrating and de novo assembly, 262,688 unigenes were produced from both libraries. Of these unigenes, 41,125 were annotated with known protein sequences in public databases. Homologous genes involved in sex determination and gonadal development pathways (Sxl-Tra/Tra-2-Dsx/Fru, Wnt4, thyroid hormone synthesis pathway, etc.) were identified. Three hundred and sixteen differentially expressed unigenes were further identified between both transcriptomes. Meanwhile, a total of 233,078 putative lncRNAs were predicted. Of these lncRNAs, 147 were differentially expressed between sexes. qRT-PCR results showed that nine lncRNAs negatively regulated the expression of eight genes, suggesting a potential role in sex differentiation. These findings will provide fundamental resources for further investigation on sex differentiation and regulatory mechanism in crustaceans.


Assuntos
Braquiúros/genética , Braquiúros/metabolismo , Ovário/metabolismo , RNA Longo não Codificante/genética , Testículo/metabolismo , Transcriptoma , Animais , Feminino , Perfilação da Expressão Gênica , Masculino , Processos de Determinação Sexual
9.
Opt Express ; 25(13): 14654-14667, 2017 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-28789049

RESUMO

This paper investigates theoretically and numerically on the electro-optical coupling (EOC) for a circular Airy beam (CAB) propagating along the optical axis of a uniaxial crystal after deducing the wave coupling equations of EOC. For a circularly polarized incident CAB, EOC can be used to generate vortex beam by coupling the incident left-handed component into the right-handed vortex component with a vortex topological charge of 2. What's more, EOC plays important role in enhancing or suppressing the abrupt autofocusing, the most important property of CABs, for both left-handed and right-handed components. Near the focal plane, EOC can result in electrically controllable optical "needle" and "cage", which shall be interesting in micromanipulation. In addition, EOC can influence or even forbid the exchange between spin angular momentum (SAM) and orbit angular momentum (OAM). For a linearly polarized incident CAB, its two Cartesian field components of the beam cannot only couple their powers to each other, but also lead to the changes of the intensity pattern and polarization distributions. The polarization state becomes spatially inhomogeneous, and possesses vortex phase with a topological charge of 2 during propagation. EOC presents a new way to control an Airy beam fast and efficiently.

10.
Appl Opt ; 56(9): 2444-2448, 2017 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-28375350

RESUMO

This paper investigates theoretically and numerically the propagation characteristics of a circular Airy beam (CAB) in a uniaxial crystal in detail. The beam loses its boundary cylindrical symmetry during propagation because of the medium anisotropy, although it propagates along the optical axis. This effect of anisotropy on the propagating beam becomes increasingly evident with the increase of the propagation distance. Another main influential factor of the propagation characteristics is the ratio of the extraordinary refractive index to the ordinary refractive index (ne/no). The more the value deviates from 1, the worse the symmetry of the beam intensity distribution becomes. The polarization becomes notably complicated, but possesses a vortex state with a topological charge of 2 during propagation. The abruptly autofocusing characteristic, the most important property of CABs, also appears when the crystal length is long enough, which is greatly different from that characteristic in isotropic media. This work is helpful for the design of optical devices based on uniaxial crystals for beams with some special wavefronts.

11.
Opt Express ; 24(7): 7299-318, 2016 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-27137020

RESUMO

Considering the measuring range limitation of a single sensor system, multi-sensor system has become essential in obtaining complete image information of the object in the field of 3D image reconstruction. However, for the traditional multi-sensors worked independently in its system, there was some point in calibrating each sensor system separately. And the calibration between all single sensor systems was complicated and required a long time. In this paper, we present a flexible 3D reconstruction method based on phase-matching in multi-sensor system. While calibrating each sensor, it realizes the data registration of multi-sensor system in a unified coordinate system simultaneously. After all sensors are calibrated, the whole 3D image data directly exist in the unified coordinate system, and there is no need to calibrate the positions between sensors any more. Experimental results prove that the method is simple in operation, accurate in measurement, and fast in 3D image reconstruction.

12.
Eur J Radiol ; 84(12): 2470-6, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26481480

RESUMO

OBJECTIVE: To investigate the value of dual-energy MDCT in spectral imaging in the differential diagnosis of chronic mass-forming chronic pancreatitis (CMFP) and pancreatic ductal adenocarcinoma (PDAC) during the arterial phase (AP) and the pancreatic parenchymal phase (PP). MATERIALS AND METHODS: Thirty five consecutive patients with CMFP (n=15) or PDAC (n=20) underwent dual-energy MDCT in spectral imaging during AP and PP. Iodine concentrations were derived from iodine-based material-decomposition CT images and normalized to the iodine concentration in the aorta. The difference in iodine concentration between the AP and PP, contrast-to-noise ratio (CNR) and the slope K of the spectrum curve were calculated. RESULTS: Normalized iodine concentrations (NICs) in patients with CMFP differed significantly from those in patients with PDAC during two double phases (mean NIC, 0.26±0.04 mg/mL vs. 0.53±0.02 mg/mL, p=0.0001; 0.07±0.02 mg/mL vs. 0.28±0.04 mg/mL, p=0.0002, respectively). There were significant differences in the value of the slope K of the spectrum curve in two groups during AP and PP (K(CMFP)=3.27±0.70 vs. K(PDAC)=1.35±0.41, P=0.001, and K(CMFP)=3.70±0.17 vs. K(PDAC)=2.16±0.70, p=0.003, respectively). CNRs at low energy levels (40-70 keV) were higher than those at high energy levels (80-40 keV). CONCLUSION: Individual patient CNR-optimized energy level images and the NIC can be used to improve the sensitivity and the specificity for differentiating CMFP from PDAC by use of dual-energy MDCT in spectral imaging with fast tube voltage switching.


Assuntos
Adenocarcinoma/diagnóstico por imagem , Carcinoma Ductal Pancreático/diagnóstico por imagem , Tomografia Computadorizada Multidetectores/métodos , Neoplasias Pancreáticas/diagnóstico por imagem , Pancreatite Crônica/diagnóstico por imagem , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Idoso , Meios de Contraste , Diagnóstico Diferencial , Feminino , Humanos , Iodo , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Curva ROC , Intensificação de Imagem Radiográfica , Estudos Retrospectivos , Sensibilidade e Especificidade
13.
Opt Express ; 22(2): 1815-23, 2014 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-24515190

RESUMO

Low driving voltage blue, green, yellow, red and white phosphorescent organic light-emitting diodes (OLEDs) with a common simply double emitting layer (D-EML) structure are investigated. Our OLEDs without any out-coupling schemes as well as n-doping strategies show low driving voltage, e.g. < 2.4 V for onset and < 3 V for 1000 cd/m2, and high efficiency of 32.5 lm/W (13.3%), 58.8 lm/W (14.3%), 55.1 lm/W (14.6%), 24.9 lm/W (13.7%) and 45.1 lm/W (13.5%) for blue, green, yellow, red and white OLED, respectively. This work demonstrates that the low driving voltages and high efficiencies can be simultaneously realized with a common simply D-EML structure.

14.
J Environ Sci (China) ; 21(6): 854-7, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19803094

RESUMO

The effects of phenanthrene (Phe) on hepatosomatic index (HSI) and hepatic enzymatic activities in hybrid tilapia (Oreochromis niloticus female x O. aureus male) were investigated via the static freshwater exposure at dosage of 50, 100, and 400 microg/L for 4-14 d. Compared with the control group, HSI was significantly decreased (P < 0.05) at 400 microg/L at day 14. Increased enzymatic activities (P < 0.05) were observed for catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) at either 100 or 400 microg/L at day 8 and 14, as well as for CAT at 50 microg/L at day 14, except for GPx at 400 microg/L at day 8. Ethoxyresorufin O-deethylase (EROD) activity was significantly increased (P < 0.05) at all dosage at day 4 as well as at 50 microg/L at day 8, but significantly decreased at either 100 or 400 microg/L at day 14 (P < 0.05). Glutathione-S-transferase (GST) activity was not affected. The results suggest that CAT, GPx, SOD and EROD, as well as HSI in tilapia may be used as the biomarkers or indexes for evaluating or monitoring the pollution of polycyclic aromatic hydrocarbons (PAHs) such as Phe.


Assuntos
Fígado , Fenantrenos/farmacologia , Tilápia/metabolismo , Poluentes Químicos da Água/farmacologia , Animais , Catalase/metabolismo , Citocromo P-450 CYP1A1/metabolismo , Relação Dose-Resposta a Droga , Feminino , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Superóxido Dismutase/metabolismo , Tilápia/anatomia & histologia
15.
Virology ; 351(2): 260-70, 2006 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-16678231

RESUMO

Recombinant baculoviruses (rBV) expressing Ebola virus VP40 (rBV-VP40) or GP (rBV-GP) proteins were generated. Infection of Sf9 insect cells by rBV-VP40 led to assembly and budding of filamentous particles from the cell surface as shown by electron microscopy. Ebola virus-like particles (VLPs) were produced by coinfection of Sf9 cells with rBV-VP40 and rBV-GP, and incorporation of Ebola GP into VLPs was demonstrated by SDS-PAGE and Western blot analysis. Recombinant baculovirus infection of insect cells yielded high levels of VLPs, which were shown to stimulate cytokine secretion from human dendritic cells similar to VLPs produced in mammalian cells. The immunogenicity of Ebola VLPs produced in insect cells was evaluated by immunization of mice. Analysis of antibody responses showed that most of the GP-specific antibodies were of the IgG2a subtype, while no significant level of IgG1 subtype antibodies specific for GP was induced, indicating the induction of a Th1-biased immune response. Furthermore, sera from Ebola VLP immunized mice were able to block infection by Ebola GP pseudotyped HIV virus in a single round infection assay, indicating that a neutralizing antibody against the Ebola GP protein was induced. These results show that production of Ebola VLPs in insect cells using recombinant baculoviruses represents a promising approach for vaccine development against Ebola virus infection.


Assuntos
Anticorpos Antivirais/sangue , Células Dendríticas/imunologia , Ebolavirus/imunologia , Insetos/virologia , Animais , Baculoviridae , Linhagem Celular , Células Dendríticas/citologia , Feminino , Regulação Viral da Expressão Gênica , Humanos , Imunoglobulina G/metabolismo , Insetos/citologia , Camundongos , Testes de Neutralização , Proteínas da Matriz Viral/imunologia
16.
Virology ; 352(1): 74-85, 2006 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-16725170

RESUMO

The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV.


Assuntos
Vacinas contra a AIDS/imunologia , Anticorpos Anti-HIV/sangue , Proteína gp41 do Envelope de HIV/imunologia , Hemaglutininas Virais/imunologia , Vírus da Influenza A Subtipo H3N2/imunologia , Proteínas Recombinantes de Fusão/imunologia , Vacinas contra a AIDS/administração & dosagem , Vacinas contra a AIDS/genética , Animais , Feminino , Genes env , Proteína gp41 do Envelope de HIV/genética , Proteína gp41 do Envelope de HIV/metabolismo , Infecções por HIV/imunologia , Infecções por HIV/prevenção & controle , HIV-1/imunologia , Hemaglutininas Virais/genética , Hemaglutininas Virais/metabolismo , Imunização , Vírus da Influenza A Subtipo H3N2/genética , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Recombinantes de Fusão/metabolismo
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