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1.
Int J Biol Macromol ; 190: 390-395, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34499953

RESUMO

Lignin-based flame retardants represent great promising next-generation flame retardants due to their sustainability, unique aromatic structure, and high charring capability. However, their applications are still limited by the compatibility, processability, and efficiency of flame retardancy. Here, a green functional lignin-based nanofiller (lignin-diethylenetriamine/red phosphorus nanoparticles, Lignin-N-P NPs) was prepared by the chemical modification and co-precipitation. After blending with the commercial acrylonitrile butadiene styrene copolymers (ABS), the physical, chemical, and flame retardant properties of the blends reveal that Lignin-N-P NPs/ABS blend has acceptable processability, mechanical properties, and significantly improved thermal stability and fire performance. Its values of peak heat release rate and total heat released per unit area were significantly dropped 67.8% and 77.5%, respectively. This study will initiate a new design for not only flame retardants but also lignin-based materials.

2.
Microb Physiol ; : 1-12, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34535588

RESUMO

Postnatal colonization and development of the gut microbiota is linked to health and growth. A comprehensive understanding of the postnatal compositional changes and development of the microbial community is helpful to understand the gut health and improve the survival rate of South China tiger cubs (Panthera tigris amoyensis). Fecal samples from three tiger cubs were collected on the day of birth in 2018 (June 17-21 [G0], July 18 [G1], July 31 [G2], and August 7 [G3]). The 16S rRNA genes of the fecal microflora were sequenced. Results showed that 38 phyla, 58 classes, 134 orders, 272 families, and 636 genera of bacteria from 3,059 operational taxonomic units were identified from 12 fecal samples. The diversity and abundance of species of group G0 were significantly higher (p < 0.05 or 0.01) than those of groups G2 and G3. The predominant phylum was Proteobacteria in groups G0 and G1 (38.85% and 48%, respectively) and Firmicutes in groups G2 and G3 (71.42% and 75.29%, respectively). At the phylum level, the abundance of Deinococcus-Thermus was significantly decreased in groups G1, G2, and G3 as compared to group G0 (p < 0.05), while that of Firmicutes was significantly increased in groups G2 and G3 (p < 0.05). At the genus level, the abundance of Faecalibacterium, Ralstonia, and unidentified Rickettsiales was significantly decreased in groups G1, G2, and G3 as compared with group G0 (p < 0.05), while that of Pseudomonas was significantly decreased in groups G2 and G3 (p < 0.05). The composition and structure of fecal microbiota of South China tiger cubs changed after birth.

3.
J Colloid Interface Sci ; 606(Pt 2): 1024-1030, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34487925

RESUMO

Currently, there is considerable interest in developing new electrode materials to construct the new-generation dual-ion batteries (DIBs) with the potential advantages of higher working voltage, good safety, low cost, and environmental friendliness. Herein, a well-known charge-transfer metal-organic compound, copper-tetracyanoquinodimethane (CuTCNQ), is synthesized and then used as an anode material, which can reversibly store Li+/Na+ ions under the lower working voltage. Consequently, the lithium/sodium-based DIBs (LDIBs/SDIBs) are constructed by coupling CuTCNQ anode with graphite cathode and their working mechanisms are also understood in detail. As expected, LDIBs exhibit a high average potential of 4.26 V, a high initial discharge capacity of 195.4 mAh g-1 at 0.1 A g-1, long cycling performance after 200 cycles with good capacity retention and excellent rate capability of 106.2 mAh g-1 at 5 A g-1. Especially, high average potential of 4.23 V and good rate capability of 34.5 mAh g-1 at 5 A g-1 could be maintained in SDIBs. These results may open a new avenue for using metal-organic compound in the field of high-performance energy-storage devices.

4.
J Vis Exp ; (173)2021 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-34338666

RESUMO

Instead of using genetic methods like RNA interference (RNAi) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease Cas9, a physical barrier was microsurgically inserted between the testes of Spodoptera litura to study the impact of this microsurgery on its growth and reproduction. After inserting aluminum foil between the testes, insect molting during metamorphosis proceeded normally. Insect growth and development were not remarkably altered; however, the number of sperm bundles changed if testes fusion was stopped by the microsurgery. These findings imply that blocking testicular fusion can influence male reproduction capability. The method can be further applied to interrupt communication between organs to study the function of specific signaling pathways. Compared to conventional surgery, microsurgery only requires freezing anesthetization, which is preferable to carbon dioxide anesthetization. Microsurgery also minimizes the surgery site area and facilitates wound healing. However, the selection of materials with specific functions needs further investigation. Avoiding tissue injury is crucial when making incisions during the operation.

5.
J Hazard Mater ; 422: 126959, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34449353

RESUMO

In this study, the mechanism of bio-electrokinetic (BIO-EK) remediation to improve the degradation of pyrene was evaluated based on an analysis of the intermediate products and the microbial community. The results show that BIO-EK remediation has a higher pyrene degradation efficiency on pyrene and its intermediate products than the bioremediation and electrokinetic (EK) remediation processes. A series of intermediate products were detected. According to the type of the intermediate products, two degradation pathways, biological metabolism and electrochemical oxidation, are proposed in the BIO-EK remediation of pyrene. Furthermore, the primary microbial taxa involved in the pollutant degradation changed, which led to variations in the functional gene components. The abundant and functional genes related to metabolism were specifically analyzed. The results indicate that the electric field promotes the expression of metabolisms associated with 14 carbohydrates, 13 lipids, 13 amino acids, five energies, and in particular, 11 xenobiotics. These results suggest that in addition to the promotion effect on the microbial metabolism caused by the electric field, BIO-EK remediation can promote the degradation of pollutants due to the coexistence of a microbial metabolic pathway and an electrochemical oxidation pathway.

6.
Soc Sci Med ; 285: 114288, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34358946

RESUMO

RATIONALE: One of the biggest challenges for mothers returning to work after childbirth is breastfeeding. Studies documented the physical health benefits of breastfeeding for mothers and children. However, research findings concerning the longitudinal effects of breastfeeding on maternal and children's mental health are mixed. OBJECTIVE: The current study investigated the longitudinal effects of the length of breastfeeding on maternal psychopathological symptoms and infants' problem behaviors, among a sample of low-income working mothers. METHODS: The sample included 285 infants and their mothers (primarily minority, low-income, and single) who returned to work 3-month postpartum, recruited from an ethnically diverse and economically disadvantaged area in a southern U.S. state. Mothers' breastfeeding behaviors were assessed four times in the first year postpartum, and mothers' psychopathological symptoms and their infants' problem behaviors were reported by mothers two times, at 12-month and 24-month postpartum. RESULTS: Path models revealed that high maternal psychopathological symptoms in infancy worsened the effect of breastfeeding on child externalizing behaviors in toddlerhood. Likewise, very high infant externalizing behaviors worsened the effect of breastfeeding on maternal hostility one year later. CONCLUSIONS: This study suggests the need for implementing prevention interventions with a lifecycle approach and continued, tailored professional breastfeeding support after hospital discharge among at-risk working mothers. Findings of this study can inform public policy by highlighting the importance of considering joint breastfeeding support and mental health counseling in the delivery of services to mothers and their infants who live in under-resourced environments and struggle with maternal psychopathology.

7.
Medicine (Baltimore) ; 100(34): e27032, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34449481

RESUMO

BACKGROUND AND AIM: Mammalian sterile 20-like kinase 1 and 2 (MST1/2) and Yes-associated protein 1 (YAP1) are the core molecules of the Hippo signaling pathway, which have been found to be unbalanced in the occurrence of tumors and promote the development of the lesions. The present study aimed to investigate the expression of MST1/2 and YAP1 proteins in triple-negative breast cancer (TNBC) and their clinicopathological significance. METHODS: Immunohistochemistry was used to detect the expression level of protein in tissues. According to the percentage of positive cells and staining intensity, the expression intensity of MST1/2 and YAP1 proteins in the tissue samples was scored, and the correlation between MST1/2 and the clinicopathological features of TNBC were discussed. RESULTS: The expression of MST1/2 and YAP1 was associated with histological grade, metastasis, lymph node metastasis stage, and tumor node metastasis stage. The overexpression of YAP1 predicted a poor prognosis in terms of overall survival and disease-free survival time. The MST1/2 expression was associated with improved overall survival and disease free survival of the patients. CONCLUSION: MST1/2 and YAP1 may be used as prognostic indicators to evaluate the recurrence of TNBC and might become one of the new targets for breast cancer treatment.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/biossíntese , Proteínas Serina-Treonina Quinases/biossíntese , Fatores de Transcrição/biossíntese , Neoplasias de Mama Triplo Negativas/patologia , Adulto , Biomarcadores Tumorais , Intervalo Livre de Doença , Feminino , Humanos , Metástase Linfática , Pessoa de Meia-Idade , Gradação de Tumores , Prognóstico , Neoplasias de Mama Triplo Negativas/mortalidade
8.
Ann Palliat Med ; 10(8): 9057-9068, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34455797

RESUMO

BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a life-threatening disease, the progression of which current drug therapy cannot reverse. This study analyzed current research hotspots and future research trends in IPF through bibliometric methods, with the aim of providing a reference for new therapeutic strategies. METHODS: Publications on IPF obtained from the Web of Science Core Collection database, The Literature Metrology Online Analysis Platform, and CiteSpace were used to analyze publication characteristics. VOSviewer was used to conduct keywords co-occurrence analysis and analyze research hotspots. RESULTS: A total of 7,016 publications related to IPF were identified from 2011 to 2020. The most contributions were from the USA and the five research institutions with the largest number of publications were all from that country. The American Journal of Respiratory and Critical Care Medicine was the most cited journal and had an incontrovertible academic impact with five of the top 10 high-cited references published in this journal. G Raghu was the academic authority in this domain in terms of both the number of publications and the most citations. By analyzing keywords, we identified three IPF research hotspot clusters, which are "clinical research", "pathogenesis research" and "diagnosis research" respectively. CONCLUSIONS: We evaluated all publications concerning IPF research in the past decade through bibliometric analysis. The current research hotspot in this field is drug therapy for the condition using nintedanib and pirfenidone. Future research will focus on conducting multi-center randomized controlled trials to explore and evaluate new therapeutic drugs for IPF. It is hoped that this study can provide information and data support for further research and the development of new therapeutic drugs.


Assuntos
Bibliometria , Fibrose Pulmonar Idiopática , Bases de Dados Factuais , Humanos , Fibrose Pulmonar Idiopática/tratamento farmacológico , Publicações , Estados Unidos
9.
J Am Chem Soc ; 143(36): 14573-14580, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34464111

RESUMO

Quantum-size metal clusters with multiple delocalized electrons could support collective plasmon excitation, and thus, theoretically, coupling of plasmons in the few-atom limit might exist between assembled metal clusters, while currently few experimental observations about this phenomenon have been reported. Here we examined the optical absorption of DNA-templated Ag nanoclusters (DNA-AgNCs) assembled through DNA hybridization and found their absorption peaks were sensitive to the assembled distances, which share common characteristics with classical plasmon coupling. Dipolar charge distribution, multiple transition contributed optical absorption, and strongly enhanced electric field simulated by time-dependent density functional theory (TDDFT) indicated the origin of the absorption of individual DNA-AgNCs is a plasmon. The consistency of the peak-shifting trend between experimental and simulation results for assembled DNA-AgNCs suggested the possible presence of plasmon coupling. Our data imply the possibility for quantum-size structures to support plasmon coupling and also show that DNA-AgNCs possess the potential to be promising materials for construction of plasmon-coupling devices with ultrasmall size, site-specific and stoichiometric binding abilities, and biocompatibility.

10.
J Dermatol Sci ; 2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34334258

RESUMO

BACKGROUND: Acne vulgaris is a prevalent skin disease lacking effective and well-tolerated treatment. An earlier study indicated that resveratrol (RVT) has therapeutic effects in acne patients through unknown mechanisms. OBJECTIVES: To evaluate the effects of RVT on linoleic acid (LA)-induced lipogenesis and peptidoglycan (PGN)-induced inflammation in cultured SZ95 sebocytes in vitro, and to investigate the underlying mechanisms. METHODS: RNA-sequencing was used to analyze the whole transcriptome. Nile red staining was used to detect intracellular neutral lipids, whereas lipidomics was used to investigate changes in the lipid profile in sebocytes. Interleukin (IL)-1ß and IL-6 mRNA and protein levels were assessed through quantitative real-time PCR and Enzyme-linked immunosorbent assay, respectively. Western blot was used to evaluate the expression of lipogenesis-related proteins, the inflammatory signaling pathway, and the AMP-activated protein kinase (AMPK) pathway. Further, specific small interfering RNA (siRNA) was used to knockdown sirtuin-1 (SIRT1) expression. RESULTS: RVT inhibited the lipogenesis-related pathway and nuclear factor-kappa B (NF-κB) signaling pathway in SZ95 sebocytes. It also downregulated LA-induced lipogenesis, the expression of lipid-related proteins, and the contents of unsaturated fatty acids. Besides, RVT promoted SIRT1 expression and deacetylation of the NF-κB p65 subunit, thereby lowering IL-1ß and IL-6 secretion under PGN induction. Furthermore, pretreatment with AMPK inhibitor Compound C abolished RVT-mediated sebosuppressive and anti-inflammation effects. Meanwhile,SIRT1 silencing abrogated the anti-inflammatory potential of RVT. CONCLUSION: In human SZ95 sebocytes, RVT exhibits sebosuppressive and anti-inflammatory effects partially through the AMPK pathway, which may justify the role of RVT treatment in acne vulgaris.

11.
Biometrics ; 2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34374075

RESUMO

Group-level brain connectome analysis has attracted increasing interest in neuropsychiatric research with the goal of identifying connectomic subnetworks (subgraphs) that are systematically associated with brain disorders. However, extracting disease-related subnetworks from the whole brain connectome has been challenging, because no prior knowledge is available regarding the sizes and locations of the subnetworks. In addition, neuroimaging data are often mixed with substantial noise that can further obscure informative subnetwork detection. We propose a likelihood-based adaptive dense subgraph discovery (ADSD) model to extract disease-related subgraphs from the group-level whole brain connectome data. Our method is robust to both false positive and false negative errors of edge-wise inference and thus can lead to a more accurate discovery of latent disease-related connectomic subnetworks. We develop computationally efficient algorithms to implement the novel ADSD objective function and derive theoretical results to guarantee the convergence properties. We apply the proposed approach to a brain fMRI study for schizophrenia research and identify well-organized and biologically meaningful subnetworks that exhibit schizophrenia-related salience network centered connectivity abnormality. Analysis of synthetic data also demonstrates the superior performance of the ADSD method for latent subnetwork detection in comparison with existing methods in various settings.

12.
Int J Mol Sci ; 22(15)2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34361090

RESUMO

Glioblastoma (GBM) is a highly lethal cancer that is universally refractory to the standard multimodal therapies of surgical resection, radiation, and chemotherapy treatment. Temozolomide (TMZ) is currently the best chemotherapy agent for GBM, but the durability of response is epigenetically dependent and often short-lived secondary to tumor resistance. Therapies that can provide synergy to chemoradiation are desperately needed in GBM. There is accumulating evidence that adaptive resistance evolution in GBM is facilitated through treatment-induced epigenetic modifications. Epigenetic alterations of DNA methylation, histone modifications, and chromatin remodeling have all been implicated as mechanisms that enhance accessibility for transcriptional activation of genes that play critical roles in GBM resistance and lethality. Hence, understanding and targeting epigenetic modifications associated with GBM resistance is of utmost priority. In this review, we summarize the latest updates on the impact of epigenetic modifications on adaptive resistance evolution in GBM to therapy.


Assuntos
Antineoplásicos/farmacologia , Neoplasias Encefálicas/tratamento farmacológico , Metilação de DNA , Resistencia a Medicamentos Antineoplásicos/genética , Epigênese Genética , Glioblastoma/tratamento farmacológico , Animais , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/patologia , Evolução Molecular , Regulação Neoplásica da Expressão Gênica , Glioblastoma/genética , Glioblastoma/patologia , Humanos
13.
Opt Lett ; 46(16): 3998-4001, 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-34388795

RESUMO

Shapes from the diffuse polarization method effectively realize the three-dimensional (3D) reconstruction of the object surface by using the polarization information of the diffuse reflection light. However, due to the nonconvexity of the particle surface, the reconstruction often falls into a local optimal solution. Indeed, the depth image obtained by the scanning electron microscope has serious stripe noise, which distorts the surface texture of the particle. In this Letter, a variable exponential function regularization method is proposed to realize 3D reconstruction for the nonconvexity of the surface and inclination of the particles. We focus on the gradient unintegrability caused by the skew and surface undulation of the specimen. An adaptive 3D reconstruction method is proposed based on variable exponential function regularization to fit the surface function of the particle. Experimental results of finite-difference time-domain simulations and actual imaging demonstrate the effectiveness of the method.

14.
Fam Process ; 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34389981

RESUMO

This study examined how individual pretreatment symptoms (depression and anxiety) predicted longitudinal, relational processes in couples therapy (the therapeutic alliance and couple satisfaction). This study included 99 heterosexual couples receiving systemic couples therapy. Partners reported their individual pretreatment symptoms of depression and anxiety before intake. Each member reported their therapeutic alliance with the therapist at the end of sessions 2-8, as well as their couple satisfaction before intake and at the end of sessions 4 and 8. A Latent Profile Analysis revealed four profiles characterizing couples on their pretreatment psychological symptoms, namely both higher (where both members of the couple had higher depressive and anxiety symptoms), female higher, male higher, and both lower profiles. Furthermore, longitudinal, dyadic multilevel models revealed that men in male higher, female higher, and both higher profiles had higher initial alliance levels, compared to men in the both lower profile. In contrast, only female partners in the both higher profile had a higher initial therapeutic alliance than those in the both lower profile. Men in the both higher profile had faster reductions in their alliance, whereas other profiles did not predict changes in male or female alliance. Additionally, the male higher and both higher profiles predicted a lower initial level of couple satisfaction in males but not females. The profiles did not predict changes in couple satisfaction, which may be a result of lowered power due to missing data. Findings were discussed in the context of systemic family theory, with clinical implications for distressed couples.

15.
J Colloid Interface Sci ; 605: 790-802, 2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34371424

RESUMO

Carbon aerogels are prepared by a thermal treating-freeze drying approach from chitosan, with glycine hydrochloride ionic liquid (IL) acting as solvent and nitrogen source. Different post-treatments such as ball milling and high temperature carbonization are employed to functionalize the obtained carbon aerogels with tuned properties, making it promising candidates as fluorescence material (NACs-Q), electrode material (FDC-800) and catalyst support (NACPd-C). NACs-Q is water-soluble quantum dot with average particle sizes of 3.8 nm, presenting excitation-/emission-independent and pH-sensitive properties, which could be used as sensor for testing acetone vapor or an "on-off-on" sensor for detections of Fe3+ and vitamin C in fruits. FDC-800 exhibits fluffy lamellar structure with developed micro-mesopores and nitrogen-containing groups on their surfaces, which is beneficial for building flexible solid-state supercapacitor with excellent performance, delivering a capacitance of 208F/g at 0.5 A/g, and achieving an energy density of 7.2 W h/kg at a power density of 50 W/kg. Moreover, NACPd-C can be used as catalyst for phenol hydrogenation, and phenol conversion of 100% with cyclohexanone selectivity of 98.3% is achieved, due to the synergetic effects of the Pd active-site, the N-containing groups, and the Lewis acid sites on the support.

16.
Mar Pollut Bull ; 172: 112872, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34454388

RESUMO

Halogenated natural products (HNPs) were identified from organic extracts of the marine sponge Hyrtios proteus from The Bahamas using gas chromatography with electron capture negative ion mass spectrometry and non-targeted gas chromatography with electron ionization mass spectrometry. The HNPs found have similar properties to anthropogenic persistent organic pollutants (POPs). Two ortho-methoxy brominated diphenyl ethers (MeO-BDEs) 2'-MeO-BDE 68 and 6-MeO-BDE 47 were the most abundant compounds. Fourteen other MeO-BDEs were detected along with several polybrominated dibenzo-p-dioxins (PBDDs) (1,3,7-triBDD, 1,3,6,8-tetraBDD and 1,3,7,9-tetraBDD) and MeO-PBDDs. Further analysis of a higher trophic level octopus (Octopus maya) from the same FAO fishing area showed that the major HNPs detected in Hyrtios proteus were also predominant. Moreover, HNPs were more than 30-fold higher in abundance than the major POPs in the octopus, i.e., polychlorinated biphenyls. Hence, Caribbean marine organisms, including those potentially used for food, harbor relatively high concentrations of HNPs.

17.
Se Pu ; 39(2): 112-117, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-34227342

RESUMO

Proteomic analysis aims at characterizing proteins on a large scale, including their relative abundance, post-translational modifications, protein-protein interactions and so on. Proteomic profiling helps to elucidate the mechanisms of disease occurrence and to discover new diagnostic markers and therapeutic targets. Mass spectrometry (MS)-based proteomic technologies have advanced to allow comprehensive qualitative and quantitative proteome profiling across a myriad proteins in cells and tissues. High-throughput proteomics is the core technique for large-scale protein characterization. With the increased demand for large cohort proteomic analysis in the biomedical research field, high-throughput proteomic analysis has become a critical issue that needs to be urgently addressed. The standard shotgun proteomic workflow comprises four steps, including sample preparation, peptide separation, MS acquisition, and data analysis. Advances in these four steps have contributed to the development of high-throughput proteomics. In this review, we aimed at summarizing the current information on the state-of-the-art development of high-throughput proteomic analysis, mainly including the following topics: (1) High-throughput, automatic proteomic sample preparation methods based on liquid-handling workstations. The automation of the proteomic sample preparation steps is essential for high-throughput proteomic analysis, which will significantly reduce variation of manual operation and sample loss by multistep sample processing. The commercial liquid handling workstations, including King FisherTM Flex, Agilent Bravo, AssayMAP Bravo, and Biomek® NXP, perform the handling steps of 96- or 384-channel microplate formats using a mechanical arm that increases the throughput and robustness of sample preparation. (2) High-throughput proteomic detection methods based on microliter-flow-rate liquid chromatography coupled with mass spectrometry (micro-flow LC-MS/MS). Nanoliter-flow-rate liquid chromatography coupled with mass spectrometry (Nano-flow LC-MS/MS) is widely used in classic proteomic research due to its excellent sensitivity, which often comes at the expense of robustness. Owing to the improved robustness and decreased injection-to-injection overheads, micro-flow LC-MS/MS has become increasingly popular in high-throughput proteomic analysis. (3) Using MS instrumentation with high sensitivity and fast scanning speed to realize in-depth proteomic analysis coupled with short chromatographic gradient separation. In recent years, new MS instrumentation continues to exhibit speed of analysis and sensitivity enables the large-scale profiling of hundreds of samples. In particular, ion mobility-based MS, such as timsTOF Pro and Exploris 480 equipped with a front-end high field asymmetric waveform ion mobility spectrometry (FAIMS), which provides fast, sensitive, and robust proteome profiling, thus shifting proteomics to the high-throughput era. (4) Artificial intelligence-, deep neural network-, and machine learning-based proteome data analysis methods. These approaches have improved comprehensive proteomic analysis efficiency. Specifically, the emergence of new algorithms and the up gradation of search engines accelerate the process of high-throughput data analysis. Additionally, the challenges and future development of high-throughput proteomics are prospected. In conclusion, high-throughput proteomic technologies are expected to gradually "transform" and become powerful tools for large cohort proteomic analysis in the near future.


Assuntos
Ensaios de Triagem em Larga Escala , Proteômica , Inteligência Artificial , Substitutos Ósseos , Cromatografia Líquida , Redes Neurais de Computação , Proteoma , Espectrometria de Massas em Tandem
18.
BMC Cancer ; 21(1): 874, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34330234

RESUMO

BACKGROUND: To examine the value of energetic-spectrum computed tomography (spectral CT) quantitative parameters in renal cell carcinoma (RCC) microvascular angiogenesis. METHODS: The authors evaluated 32 patients with pathologically confirmed RCC who underwent triple-phase contrast-enhanced CT with spectral CT imaging mode from January 2017 to December 2019. Quantitative parameters include parameters derived from iodine concentration (IC) and water concentration (WC) of 120 keV monochromatic images. All specimens were evaluated including the microvascular density (MVD), microvascular area (MVA) and so on. The correlation between IC and WC (including average values and random values) with microvascular parameters were analyzed with Pearson or Spearman rank correlation coefficients. RESULTS: The MVD of all tumors was 26.00 (15.00-43.75) vessels per field at × 400 magnification. The MVD of RCC correlated positively with the mean IC, mean WC, mean NWC, mean NIC, random IC, random NIC in renal cortical phase, WCD1, WCD2, NWCD2 and ICD1 (Spearman rank correlation coefficients, r range, 0.362-0.533; all p < 0.05). The MVA of all tumors was (16.16 ± 8.98) % per field at × 400 magnification. The MVA of RCC correlated positively with the mean IC, mean WC, mean NWC, mean NIC, random IC, random NIC in renal cortical, mean WC and mean NWC in renal parenchymal phase, WCD1, WCD2, WCD3, NWCD2, and NWCD3 (Pearson or Spearman rank correlation coefficients, r range, 0.357-0.576; all p < 0.05). Microvascular grading correlated positively with the mean NWC, mean NIC and random NIC in renal cortical phase, mean NWC in renal parenchymal phase, NWCD2, WCD3, NWCD3, NICD2 and NICD3 (Spearman rank correlation coefficients, r range, 0.367-0.520; all p < 0.05). As for tumor diameter (55.19 ± 19.15), µm, only NWCD3 was associated with it (Spearman rank correlation coefficients, r = 0.388; p < 0.05). CONCLUSIONS: ICD and WCD of spectral CT have a potential for evaluating RCC microvascular angiogenesis. MVD, MVA and microvascular grade showed moderate positive correlation with ICD and WCD. ICD displayed more relevant than that of WCD. The parameters of renal cortical phase were the best in three phases. NICD and NWCD manifested stronger correlation with microvascular parameters than that of ICD and WCD.

19.
J Phys Chem B ; 125(27): 7537-7545, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-34228929

RESUMO

Recently presented as a rapid and eco-friendly manufacturing method for thermoset polymers and composites, frontal polymerization (FP) experiences thermo-chemical instabilities under certain conditions, leading to visible patterns and spatially dependent material properties. Through numerical analyses and experiments, we demonstrate how the front velocity, temperature, and instability in the frontal polymerization of cyclooctadiene are affected by the presence of poly(caprolactone) microparticles homogeneously mixed with the resin. The phase transformation associated with the melting of the microparticles absorbs some of the exothermic reaction energy generated by the FP, reduces the amplitude and order of the thermal instabilities, and suppresses the front velocity and temperatures. Experimental measurements validate predictions of the dependence of the front velocity and temperature on the microparticle volume fraction provided by the proposed homogenized reaction-diffusion model.


Assuntos
Polímeros , Polimerização , Temperatura
20.
J Org Chem ; 86(15): 10427-10439, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34313431

RESUMO

The first application of 3-alkyl-2-vinylindoles in catalytic asymmetric dearomative cycloadditions was established by chiral phosphoric acid (CPA)-catalyzed (2+3) cycloaddition with azoalkenes, leading to the generation of chiral pyrroloindolines bearing two tetrasubstituted stereogenic centers in good yields (61-96%) and excellent stereoselectivities (all >95:5 dr, 86-99% ee). This reaction has realized the first enantioselective dearomative cycloaddition of 3-alkyl-2-vinylindoles, which brings a new reactivity to this class of vinylindoles and will enrich the chemistry of 3-alkyl-2-vinylindoles. In addition, this approach has provided a useful strategy for the construction of enantioenriched pyrroloindoline skeletons bearing two tetrasubstituted stereogenic centers. More importantly, the bioassay of these chiral pyrroloindolines has revealed that some compounds exhibit strong anti-cancer activity against Hela and MCF-7 cell lines, which will be helpful for discovering anti-cancer drug candidates.

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