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1.
Mol Plant ; 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35524410

RESUMO

Chinese sprangletop (Leptochloa chinensis (L.) Nees), belonging to the grass subfamily Chloridoideae, is one of the most notorious weeds in the rice ecosystems. Here we report a chromosome-scale reference genome assembly and a genomic variation map of the tetraploid L. chinensis. The L. chinensis genome is contributed by two diploid progenitors that diverged ∼10.9 million years ago, and its two subgenomes display neither fractionation bias nor overall gene expression dominance. Comparative genomic analyses reveal substantial genome rearrangements in L. chinensis after its divergence from the common ancestor of Chloridoideae, and together with and transcriptome profiling, demonstrate the important contribution of the tetraploidization to the gene sources for the L. chinensis herbicide resistance. Population genomic analyses of 89 accessions from China uncover that L. chinensis collected from southern/southwestern provinces have substantially higher nucleotide diversity than those from the middle and lower reaches of the Yangtze River, suggesting the spread route of L. chinensis in China from the southern/southwestern provinces to the middle and lower reaches of the Yangtze River. During this spread, L. chinensis shows significantly increased herbicide resistance, accompanied by the selection of numerous genes involved in herbicide resistance. Together, our study provides valuable genomic resources for the management of L. chinensis and insights into the herbicide resistance as well as the origin and adaptive evolution of L. chinensis.

2.
Artigo em Inglês | MEDLINE | ID: mdl-35546428

RESUMO

ABSTRACT: A technique using the single-string, closed-loop fixation method to reposit dislocated triple-looped haptic IOL-capsular bag complexes is described. The long needle or curved needle with 10-0 / 8-0 polypropylene suture and 27 / 30g needle were used as the guide needle to pass through the fenestrated haptics for two times. The scleral interlaminar course was used as the fixed point. last, A fixation knot was created in the sclerotomy by the two ends of the thread to close the suture loop for IOL fixation. Another knot was created about 2 to 3 mm from the exiting point and was intrasclerally anchored by the aid of the attached needle.Four eyes from 4 consecutive patients were studied retrospectively; During all follow-up visits, the IOLs were well centered and stable, No suture erosion, hypotony, scleral atrophy, chronic inflammation, retinal tears, and/or detachments were observed.

3.
Mar Genomics ; 63: 100953, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35568403

RESUMO

Thalassospira sp. SW-3-3 is a bacterial strain isolated from deep seawater of the Pacific Ocean at a water depth of 3112 m. It is a Gram-negative, aerobic, and curved rod-shaped bacterium belonging to the family Thalassospiraceae. In this study, we report the complete genome sequence of strain SW-3-3. It has a circular chromosome with a size of 4,764,478 bp and a G + C content of 54.7%. The genome contains 4296 protein-coding genes, 63 tRNA genes, and 12 rRNA genes. Genomic analysis shows that strain SW-3-3 contains genes and catalytic pathways relevant to phthalate metabolism. Phthalates are well-known emerging contaminants that are harmful to environments and human health. They are chemically stable compounds that are widely used in plastic products and are pervasive in our life. With the discharge of plastic pollutants, a huge number of phthalate compounds enter the ocean. The genetic information of strain SW-3-3 suggests that it has the potential to metabolize phthalates. There are 9 key enzymes in the metabolization pathway, and phthalates are finally catalyzed to produce succinyl-CoA which is further degraded through the tricarboxylic acid (TCA) cycle pathway. This genomic analysis will be helpful for further understanding of the applications of strain SW-3-3 in the remediation of phthalate pollution.

4.
Front Med (Lausanne) ; 9: 818144, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35445045

RESUMO

Gypenosides (GPs), obtained from Gynostemma pentaphyllum (Thunb.) Makino, have been traditionally prescribed to treat metabolic disorders in Asians. This study assessed whether GPs could prevent lithogenic diet (LD)-induced cholesterol gallstone (CG) formation and enhance CG dissolution in mice. Gallstone-susceptible C57BL/6J mice were fed an LD or normal chow, with or without GPs. Bile acids (BAs) in gallbladder bile were analyzed by liquid chromatography-tandem mass spectrometry. Differentially expressed hepatic genes were identified by RNA sequencing, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. GPs were found to prevent LD-induced CG formation and to dissolve pre-existing LD-induced CGs. GPs reduced total cholesterol levels and increased BA levels in bile, as well as reducing the BA Hydrophobicity Index, ratio of 12α-hydroxylated (12α-OH) to non-12α-OH BAs, and Cholesterol Saturation Index in gallbladder bile. GO and KEGG pathway enrichment analyses indicated that GPs-induced genes were involved in BA biosynthesis and cholesterol metabolism. GPs increased the hepatic expression of genes encoding the cytochrome P450 (Cyp) enzymes Cyp7a1, Cyp7b1, and Cyp8b1, while decreasing the hepatic expression of genes encoding the adenosine triphosphate-binding cassette (Abc) transporters Abcg5 and Abcg8. GPs may be a promising strategy for preventing and dissolving CGs.

5.
Res Int Bus Finance ; 61: 101666, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35475264

RESUMO

Combining the spillover index approach and LASSO-VAR method, we construct the spillover network of 19 specific countries' economic policy uncertainty (EPU). Then we deconstruct the constructed network into four blocks by the block models, the impacts of COVID-19 on EPU spillover effects between each country and blocks is analyzed gradually. The results reveal that: (1) The transnational contagion of EPU is significant, and the spillover network of policy uncertainty is time-varying. (2) EPU networks can be divided into four different blocks by block models. The role of blocks and the spatial spillover transmission path between blocks are different in different periods. (3) The new infection cases and deaths of COVID-19 have a significant effect on reception and transmission directional EPU spillovers, while there is no significant impact on net spillovers. The international movement restrictions during the period of COVID-19 significantly increase the directional and net EPU spillovers. Our findings have some implications for policy-makers and market regulators in the context of the COVID-19 pandemic.

6.
Sensors (Basel) ; 22(8)2022 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-35459014

RESUMO

In recent years, we have witnessed the emergence of the implementation and integration of significant working solutions in transportation, especially within the smart city concept. A lot of cities in Europe and around the world support this initiative of making their cities smarter for enhanced mobility and a sustainable environment. In this paper, we present a case study of Tartu city, where we developed and designed a daily real-time system for extracting and performing a modal split analysis. Our web-based platform relied on an optimization approach for calibrating our simulation in order to perform the analysis with the use of real data streams from IoT devices installed around the city. The results obtained from our system demonstrated acceptable performance versus the quality of the available data source. In addition, our platform provides downloadable OD matrices for each mode of mobility for the community.

7.
PLoS One ; 17(4): e0266766, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35413087

RESUMO

Our understanding of interactions among intestinal helminths, gut microbiota and host is still in its infancy in fish. In this study, the effects of Schyzocotyle acheilognathi infection on the intestinal microbiota, growth and immune reactions of grass carp were explored under laboratory conditions. 16S rDNA amplification sequencing results showed that S. acheilognathi infection altered the composition of intestinal microbiota only at the genus level, with a significant increase in the relative abundance of Turicibacter and Ruminococcus (P < 0.05) and a significant decrease in the relative abundance of Gordonia, Mycobacterium and Pseudocanthomonas (P < 0.05). Schyzocotyle acheilognathi infection had no significant effect (P > 0.05) on the alpha diversity indices (including Chao1, ACE, Shannon, Simpson index) of intestinal microbiota in grass carp, but PERMANOVA analysis showed that microbial structure significantly (P < 0.01) differed between hindgut and foregut. PICRUST prediction showed that some metabolism-related pathways were significantly changed after S. acheilognathi infection. The relative abundance of Turicibacter was positively correlated with the fresh weight of tapeworm (foregut: r = 0.48, P = 0.044; hindgut: r = 0.63, P = 0.005). There was no significant difference in the body condition of grass carp between the S. acheilognathi infected group and the uninfected group (P > 0.05). Intestinal tissue section with HE staining showed that S. acheilognathi infection severely damaged the intestinal villi, causing serious degeneration, necrosis and shedding of intestinal epithelial cells. The real-time fluorescent quantitative PCR results showed that S. acheilognathi infection upregulated the mRNA expression of the immune-related genes: Gal1-L2, TGF-ß1 and IgM.


Assuntos
Carpas , Cestoides , Infecções por Cestoides , Doenças dos Peixes , Microbioma Gastrointestinal , Animais , Carpas/metabolismo , Infecções por Cestoides/parasitologia , Dieta , Doenças dos Peixes/parasitologia , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Intestinos/microbiologia
8.
Front Public Health ; 10: 746591, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35309209

RESUMO

Background: Although there is a growing research base on low back pain, the bibliometric literature related to it is deficient. The aim of this study was to conduct a bibliometric and visualization analysis of low back pain and to provide a broad view of the current trends in LBP research and a potential guide in this discipline. Methods: The authors searched the Web of Science to extract publications regarding low back pain, and found a total of 12,249 publications during a period of 22 years, among which 12,242 were eligible. We classified and analyzed publications such as total citations, average citations per item, H-index, research types, countries/regions, institutions, and journals using standard bibliometric indicators. Bibliometric approaches (VOSviewer1.6.13 and CiteSpace 5.8.3) were also available for gathering information and explore the trends of research. Results: Conspicuously, over the past 22 years, an increasing number of scholars have specialized in the research of LBP, exerting the boom in articles. The largest number of document type was that of articles. Under modern conditions, regional distinction existed in the research of low back pain and developed countries preceded others. Research individuals and institutions were preoccupied by respective aspects. Visualization analysis provided objective information for potential collaborators and cooperative institutions. Furthermore, most burst keywords varied during different periods. Conclusions: The map of research on LBP obtained by our analysis is expected to help researchers to efficiently and effectively explore LBP.


Assuntos
Dor Lombar , Bibliometria , Humanos
9.
J Cell Mol Med ; 2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35322540

RESUMO

Autologous adipose tissue is an ideal soft tissue filling material, and its biocompatibility is better than that of artificial tissue substitutes, foreign bodies and heterogeneous materials. Although autologous fat transplantation has many advantages, the low retention rate of adipose tissue limits its clinical application. Here, we identified a secretory glycoprotein, leucine-rich-alpha-2-glycoprotein 1 (LRG-1), that could promote fat graft survival through RAB31-mediated inhibition of hypoxia-induced apoptosis. We showed that LRG-1 injection significantly increased the maintenance of fat volume and weight compared with the control. In addition, higher fat integrity, more viable adipocytes and fewer apoptotic cells were observed in the LRG-1-treated groups. Furthermore, we discovered that LRG-1 could reduce the ADSC apoptosis induced by hypoxic conditions. The mechanism underlying the LRG-1-mediated suppression of the ADSC apoptosis induced by hypoxia was mediated by the upregulation of RAB31 expression. Using LRG-1 for fat grafts may prove to be clinically successful for increasing the retention rate of transplanted fat.

10.
ACS Omega ; 7(9): 7825-7836, 2022 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-35284738

RESUMO

Wang-Bi capsule (WB) is a traditional Chinese medicine (TCM)-based herbal formula, and it has been used in the treatment of rheumatoid arthritis (RA) in China for many years. Additionally, WB is also used as a supplement to the treatment of osteoarthritis (OA) in clinical practice. Our research aimed to reveal the therapeutic effects and underling mechanism of WB on RA and OA through computational system pharmacology analysis and experimental study. Based on network pharmacology analysis, a total of 173 bioactive compounds interacted with 417 common gene targets related to WB, RA, and OA, which mainly involved the PI3K-Akt signaling pathway. In addition, the serine-threonine protein kinase 1 (AKT1) might be a core gene protein for the action of WB, which was further emphasized by molecular docking. Moreover, the anti-inflammatory activity of WB in vitro was confirmed by reducing NO production in lipopolysaccharide (LPS)-induced RAW264.7 cells. The anti-RA and OA effects of WB in vivo were confirmed by ameliorating the disease symptoms of collagen II-induced RA (CIA) and monosodium iodoacetate-induced OA (MIA) in rats, respectively. Furthermore, the role of the PI3K-Akt pathway in the action of WB was preliminarily verified by western blot analysis. In conclusion, our study elucidated that WB is a potentially effective strategy for the treatment of RA and OA, which might be achieved by regulating the PI3K-Akt pathway. It provides us with systematic insights into the effects and mechanism of WB on RA and OA.

11.
Food Chem ; 384: 132589, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35258001

RESUMO

In this study, chitosan-based bigels were fabricated, where glycerol monolaurate was added in MCT oil to produce a gelled lipid phase and cinnamaldehyde was included in the lipid phase in order to act as a crosslinking agent. The synergistic effect of pH on chemical crosslinking effects was investigated. The potential of using these bigels as an alternative to cream was also investigated. The pH of the aqueous phase played an important role in controlling the extent of the Schiff-base reaction promoted by cinnamaldehyde. At pH 3.8, the bigels formed were homogenous but at pH 5.0 and 5.5 they exhibited phase separation, which highlighted the importance of chemical crosslinking. To better mimic the properties of real cream, span 80 was added to create a more homogeneous and smoother structure of the bigels. These bigels might provide a healthy and more sustainable alterative to food products that contain plastic fats, like cream.


Assuntos
Quitosana , Acroleína/análogos & derivados , Quitosana/química , Hidrogéis/química , Lauratos , Monoglicerídeos
12.
J Hazard Mater ; 431: 128537, 2022 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-35278942

RESUMO

The dissemination of antibiotic resistance genes (ARGs) in pathogens is becoming a pervasive global health threat, to which the importance of the environment attracts more and more attention. However, how natural minerals affect ARGs transfer in pathogens is still unclear. In this study, the concentration and size effects of hematite on the ARGs conjugative transfer to a common zoonotic pathogen Escherichia coli O157:H7 and underlying mechanisms were explored. Results revealed that bulk hematite reduced the conjugation of resistant plasmids by inhibiting cell growth at any concentration (1-100 mg/L), different from nano-hematite. Low concentrations of nano-hematite (≤ 25 mg/L) induced significant increases in conjugative transfer frequency of 1.83-4.49 folds, while its high concentrations (50 and 100 mg/L) showed no impact, compared with the control group. This low-concentration effect was likely attributed to the increased intracellular ROS level, the reduced intercellular repulsion by increasing the extracellular polymeric substances production and cell surface hydrophobicity, the formation of transfer channels and the increased membrane permeability evidenced by significant changes in gene expression level, and the increased proton motive force by increasing the transmembrane potential of recipients. These findings shed light on potential health risks caused by nano minerals-mediated ARGs dissemination in pathogens in the environment.


Assuntos
Antibacterianos , Genes Bacterianos , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos/genética , Compostos Férricos , Transferência Genética Horizontal , Plasmídeos/genética
14.
Sci Total Environ ; 823: 153619, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35124032

RESUMO

The treatment of hypersaline oilfield wastewater (HSOW) is a challenge due to its complex composition and low biodegradability, especially in coastal areas. In this study, an HSOW treatment system of gas flotation and biochemistry technology combined with constructed wetland (CW) was investigated. The combined treatment system could efficiently remove COD, NH4+-N and oil under high salinity (1.36-2.21 × 104 mg/L), with average removal rates of 98.5%, 99.9% and 96%, respectively. Meanwhile, different salinity shaped particular community structures and functions. The abundance of Marivita, Parvibaculum, etc. was highly correlated with salinity. Co-occurrence network resulted that the microorganisms were highly interconnected, and formed a functional group of petroleum degrading. Pseudomonas, Rosevarius, Alternaria, etc. were the key genera. Moreover, functional prospected revealed that high salinity reduced the energy metabolism activity. This study will optimize the combined process and provide the basis for further extraction of high-efficiency degradation strains for HSOW enhanced treatment.


Assuntos
Poluentes Ambientais , Purificação da Água , Campos de Petróleo e Gás , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Áreas Alagadas
15.
J Dig Dis ; 23(3): 157-165, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35150060

RESUMO

OBJECTIVE: To investigate the incidence of primary gallbladder and biliary tract cancer, mortality and disability-adjusted life years (DALY) of the global burden from 1990 to 2017. METHODS: Data of 195 countries and territories from 1990 to 2017 were extracted from the Global Health Data Exchange. The age-standardized incidence rate (ASIR) and estimated annual percentage change (EAPC) were employed to quantify trends in the incidence of primary gallbladder and biliary tract cancer. The age-standardized death rate (ASDR), age-standardized DALY and their corresponding EAPC were used to evaluate mortality trends. RESULTS: The global incidence of primary gallbladder and biliary tract cancer rose by 75.9% from 119 900 cases in 1990 to 210 900 cases in 2017. The highest ASIR was observed in Chile (10.8 per 100 000 in 2017), followed by Japan and South Korea. Regions with the highest social development index (SDI) quintile also had the highest death cases associated with primary gallbladder and biliary tract cancer in 2017 (60 100, 95% UI 55 800-62 700). A high body mass index (BMI) was found to be closely related to age-standardized deaths and age-standardized DALY in most of the regions analyzed. CONCLUSIONS: Primary gallbladder and biliary tract cancer remains a serious threat to global public health, especially in high-SDI countries. The ASDR and age-standardized DALY decreased from 1990 to 2017. A high BMI may be associated with this cancer burden.


Assuntos
Neoplasias do Sistema Biliar , Carga Global da Doença , Neoplasias do Sistema Biliar/epidemiologia , Índice de Massa Corporal , Vesícula Biliar , Humanos , Incidência , Anos de Vida Ajustados por Qualidade de Vida , Fatores de Risco
16.
Antioxidants (Basel) ; 11(2)2022 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-35204181

RESUMO

Targeting ferritin via autophagy (ferritinophagy) to induce ferroptosis, an iron- and reactive oxygen species (ROS)-dependent cell death, provides novel strategies for cancer therapy. Using a ferroptosis-specific inhibitor and iron chelator, the vulnerability of triple-negative breast cancer (TNBC) MDA-MB-231 cells to ferroptosis was identified and compared to that of luminal A MCF-7 cells. Saponin formosanin C (FC) was revealed as a potent ferroptosis inducer characterized by superior induction in cytosolic and lipid ROS formation as well as GPX4 depletion in MDA-MB-231 cells. The FC-induced ferroptosis was paralleled by downregulation of ferroportin and xCT expressions. Immunoprecipitation and electron microscopy demonstrated the involvement of ferritinophagy in FC-treated MDA-MB-231 cells. The association of FC with ferroptosis was strengthened by the results that observed an enriched pathway with differentially expressed genes from FC-treated cells. FC sensitized cisplatin-induced ferroptosis in MDA-MB-231 cells. Through integrated analysis of differentially expressed genes and pathways using the METABRIC patients' database, we confirmed that autophagy and ferroptosis were discrepant between TNBC and luminal A and that TNBC was hypersensitive to ferroptosis. Our data suggest a therapeutic strategy by ferroptosis against TNBC, an aggressive subtype with a poor prognosis.

17.
J Ethnopharmacol ; 288: 114969, 2022 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-34999146

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional herb couple Angelicae pubescentis radix (APR) and Notopterygii rhizoma et radix (NRR), composition of two traditional Chinese medicinal herbs, has been used clinically in China for the treatment of rheumatoid arthritis (RA) over years. APR and NRR contain coumarins and phenolic acids, which have been reported to have analgesic and anti-inflammatory activities. AIM OF THE STUDY: The active ingredients combination (AIC) and potential therapeutic mechanism of APR and NRR (AN) herb couple remain unclear. Therefore, the present study aimed to identify the AIC and elucidate the underlying mechanism of AIC on RA. MATERIALS AND METHODS: Firstly, a novel strategy of in vitro experiments, computational analysis, UPLC-QTOF-MS and UPLC-QQQ-MS was established to confirm the optimum ratio of AN herb couple samples and identified the AIC. Then, the anti-arthritis effects of the optimal herb couple and AIC were studied with Collagen II induced rheumatoid arthritis (CIA) rats in vivo. Finally, an integrated model of network pharmacology, metabolomics, gut microbiota analysis and biological techniques were applied to clarify the underlying mechanism through a comprehensive perspective. RESULTS: AN7:3 herb couple was regarded as the optimal ratio of AN herbal samples, and AIC was screened as osthole, columbianadin, notopterol, isoimperatorin, psoralen, xanthotoxin, bergapten, nodakenin and bergaptol respectively. Additionally, AIC exerted similar therapeutic effects as AN 7:3 in CIA rats. Moreover, AIC ameliorated RA might via regulating MAPK signaling pathway, altering metabolic disorders and gut microbiome involved autoimmunity. CONCLUSIONS: our findings provided scientific evidence to support that AIC of AN herb couple could be used as a prebiotic agent for RA. Importantly, this research provided a systematic and feasible strategy to optimize the proportion of medicinal materials and screen AIC from multi-component traditional Chinese herb couples or Chinese medicine formulae. Moreover, it provided a comprehensive perspective to discover AIC, clarify the overall effects and understand the mechanisms for natural products through the perspective of database and multi-omics integration.


Assuntos
Apiaceae/química , Artrite Experimental/tratamento farmacológico , Artrite Reumatoide/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Angelica/química , Animais , Antirreumáticos/administração & dosagem , Antirreumáticos/isolamento & purificação , Antirreumáticos/farmacologia , Colágeno Tipo II , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Feminino , Microbioma Gastrointestinal , Células Endoteliais da Veia Umbilical Humana , Humanos , Camundongos , Células RAW 264.7 , Ratos , Ratos Wistar
18.
Huan Jing Ke Xue ; 43(1): 416-423, 2022 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-34989526

RESUMO

For the ambient and low-temperature operation of the anaerobic ammonium oxidation (ANAMMOX) process, sequence batch biofilm reactors (SBBR) with non-woven fabric and modified polyurethane foam as carriers, respectively, were used to treat nitrogenous wastewater. Nitrogen removal performances of both reactors were investigated while the temperature decreased from 35℃ to 15℃. When the nitrogen loading rate (NLR) was 110 mg·(L·d)-1, the average total nitrogen (TN) removal rate of the non-woven fabric reactor increased from 71.16% (35℃) to 76.19% (30℃) and then decreased to 61.11% (15℃). The average TN removal rate of the modified polyurethane foam reactor increased from 78.71% (35℃) to 81.75% (20℃) and then decreased to 75.68% (15℃). Compared with the non-woven fabric, the modified polyurethane foam provided a more stable nitrogen removal performance and could be used as the preferred carrier. High-throughput sequencing of the modified polyurethane foam biofilm indicated that Candidatus Kuenenia was the dominant ANAMMOX genus, with relative abundances of 8% (35℃), 5% (25℃), and 16% (15℃). Candidatus Kuenenia still grew and enriched at low temperatures. The decrease in temperature significantly affected the biofilm microbial community structure.


Assuntos
Compostos de Amônio , Nitrogênio , Anaerobiose , Biofilmes , Reatores Biológicos , Desnitrificação , Oxirredução , Esgotos , Temperatura
19.
Acad Radiol ; 29 Suppl 2: S47-S52, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-33189549

RESUMO

RATIONALE AND OBJECTIVES: To compare the ability of radiomics models including the perinodular parenchyma and standard nodular radiomics model in lung cancer diagnosis of solid pulmonary nodules smaller than 2 cm. MATERIALS AND METHODS: In this retrospective study, the computed tomography (CT) scans of 206 patients with a lung nodule from a single institution in 2012-2019 were collected. For each nodule, four volumes of interest were defined using the gross tumor volume (GTV) and peritumoral volumes (PTVs) of 5, 10, and 15 mm around the tumor. RESULTS: Radiomics models created from GTV, GTV plus 5 mm of PTV, GTV plus 10 mm of PTV, and GTV plus 15 mm of PTV achieved AUCs of 0.89, 0.81, 0.81, and 0.73, respectively, in the validation cohort for the diagnostic classification of benign and malignant pulmonary nodules. The performance of the models gradually decreased as the PTV increased. Wavelet features were the primary features identified in optimal radiomics signatures (2/3 in R, 4/5 in GTV plus 5 mm PTV, 3/4 in GTV plus 10 mm PTV, 2/3 in GTV plus 15 mm PTV). CONCLUSION: Our study indicated that the radiomics signatures of GTV had a good prediction ability in distinguishing benign and malignant solid pulmonary nodules smaller than 2 cm on CT. However, the radiomics feature of the surrounding parenchyma of the nodule did not enhance the effectiveness of the diagnostic model.


Assuntos
Neoplasias Pulmonares , Nódulos Pulmonares Múltiplos , Humanos , Pulmão/patologia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Nódulos Pulmonares Múltiplos/diagnóstico por imagem , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
20.
J Cancer ; 13(5): 1468-1479, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35371324

RESUMO

The morbidity and mortality of lung cancer are among the forefront of various cancers, and it is one of the major cancers that seriously threaten human life and health. It is well known that the abundant angiogenesis and lymphangiogenesis in tumor tissues play an important role in tumor growth and metastasis. In addition, The epithelial-mesenchymal transition (EMT), which facilitates the tumor cell metastasis and invasion, is triggered by many stimuli, such as matrix metalloproteinases 2 (MMP2), MMP9, and transforming growth factor-ß1 (TGF-ß1). At present, various studies have confirmed that both moderate intensity constant load exercise (MICE) and high-intensity interval exercise (HIIE) have a positive therapeutic effect on the treatment of lung cancer, delaying the progression of lung cancer. However, little is currently known regarding whether its specific treatment mechanism is related to blood vessels, lymphatic vessels, and EMT. Indeed, we found an increase in angiogenesis and lymphangiogenesis in lung cancer tissues. However, compared to high-intensity interval exercise, moderate intensity constant load exercise can significantly reduce tumor growth in the lung independent of blood vessels and lymphatic vessels. It is worth noting that moderate intensity constant load exercise can also reduce the level of MMP9 in lung cancer tissues, which may control tumor metastasis to a certain extent. In addition, high-intensity interval exercise reduces the expression of MMP2, but it tends to enhance EMT and activate TGF-ß1. Taken together, our findings suggest that, whether it is tumor growth or metastasis, moderate intensity constant load exercise has a better therapeutic effect on lung cancer than high-intensity interval exercise.

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