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1.
World J Surg ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31576441

RESUMO

BACKGROUND: The cost of thyroidectomy varies across the USA, while the causes of this variation are poorly understood. We examined the cost of inpatient thyroidectomy among National Cancer Institute-designated cancer centers nationwide to determine why it differs. METHODS: A retrospective study of inpatient thyroidectomies was performed using the Vizient Clinical Data Base. Fifty-two of 70 eligible hospitals were grouped into five geographic regions (Mid-Atlantic and New England, East Central, South Atlantic, West Central, and Mountain and Pacific). We identified drivers of cost variation in the five geographic regions and used risk adjustment model to evaluate the rationality of cost from each hospital. RESULTS: Male sex, more extended hospital stays, and occurrence of complications were consistently associated with increased costs in all regions. Also, the cost was significantly lower in the Mid-Atlantic and New England region. The higher than expected costs did not correlate well with the case mix index among hospitals (p = 0.289), but the lower than expected costs were more common in high-volume hospitals. The average length of stay was the shortest in high-volume hospitals, which might account for the lower cost in the Mid-Atlantic and New England region; however, the overages of costs still varied widely among hospitals in all regions even if the length of stay was adjusted. CONCLUSIONS: Cost variation may result from both patient-related factors and volume-related practice pattern differences among hospitals. A more standard of care and charge transparency is still needed for patients seeking affordable care at cancer centers.

2.
World J Surg ; 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31493191

RESUMO

BACKGROUND: The American Joint Committee on Cancer 8th edition staging manual for adrenocortical carcinoma (ACC) redefines T stage to include large vessel invasion (T4, previously undescribed) and restricts stage IV to those with distant metastases. We evaluated the prognostic power of the 8th edition. METHODS: Patients with ACC treated between January 1, 2000, and December 31, 2015, were identified. Overall survival (OS) was compared using Kaplan-Meier and Cox proportional hazard models. RESULTS: Of 290 patients evaluated, the change in T stage nomenclature impacted 13 (4.5%) who were previously categorized as T3; 61 had large vessel involvement but were already T4 based on invasion of adjacent organs. The restriction of stage IV to patients with distant metastases downstaged 41 (14.1%; T4N0M0 or T3-4N1M0) to stage III. In the 7th edition, the hazard ratio (HR) for death was similar between patients with stage II and III disease, with 5-year OS 66.7%, 54.4%, 57.2%, and 14.0% (stages I, II, III, and IV, respectively). In the 8th edition, stages I and II remain unchanged, with 5-year OS for stage III and IV 44.1% and 9.2%, respectively. The c-index for the 7th and 8th editions was similar (83.4 and 82.7, respectively). CONCLUSIONS: While 8th edition changes impact a relatively small proportion of ACC patients, they represent progress toward a common staging system that accurately reflects prognosis. In the 8th edition, the inclusion of patients with T4 tumors or nodal disease as stage III rather than IV results in improved stratification between stages II and III.

3.
Molecules ; 24(15)2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31366025

RESUMO

In the present work, we developed a simple and rapid sample preparation method for the determination of neonicotinoid pesticides in honey based on the matrix-induced sugaring-out. Since there is a high concentration of sugars in the honey matrix, the honey samples were mixed directly with acetonitrile (ACN)-water mixture to trigger the phase separation. Analytes were extracted into the upper ACN phase without additional phase separation agents and injected into the HPLC system for the analysis. Parameters of this matrix-induced sugaring-out method were systematically investigated. The optimal protocol involves 2 g honey mixed with 4 mL ACN-water mixture (v/v, 60:40). In addition, this simple sample preparation method was compared with two other ACN-water-based homogenous liquid-liquid extraction methods, including salting-out assisted liquid-liquid extraction and subzero-temperature assisted liquid-liquid extraction. The present method was fully validated, the obtained limits of detection (LODs) and limits of quantification (LOQs) were from 21 to 27 and 70 to 90 µg/kg, respectively. Average recoveries at three spiked levels were in the range of 91.49% to 97.73%. Precision expressed as relative standard deviations (RSDs) in the inter-day and intra-day analysis were all lower than 5%. Finally, the developed method was applied for the analysis of eight honey samples, results showed that none of the target neonicotinoid residues were detected.

4.
Genes (Basel) ; 10(3)2019 03 08.
Artigo em Inglês | MEDLINE | ID: mdl-30857170

RESUMO

Chinese kale is a native vegetable in Southern China and the flowering stalk is the most commonly used edible part due to its high glucosinolate content and other nutritional qualities. The GTR protein played important roles in the glucosinolate transport process. In this study, three BocGTR1 genes were cloned from Chinese kale for the first time. Their gene structure, physicochemical properties, signal peptides, transmembrane structures, functional domains, second and third-order protein structures, and phylogenetic relationships were predicted. The expression levels of BocGTR1a and BocGTR1c were much higher than those of BocGTR1b in various tissues, especially in leaves and buds. In addition, the expression patterns of three genes were examined under various abiotic stresses or hormone treatment, including those induced by wounding, heat stress, methyl jasmonate, salicylic acid, salt, and MgCl2 treatment. BocGTR1a and BocGTR1c were strongly induced by wounding and heat stress. The expression of BocGTR1a and BocGTR1c was significantly silenced in plants transformed by RNAi technology. Total glucosinolate content was significantly decreased in mature leaves and increased in roots of RNAi-transformed plants compared to wild-type plants. In addition, we found that BocGTR1a and BocGTR1c may participate in glucosinolate accumulation in different tissues with a selection for specific glucosinolates. These results indicated that BocGTR1a and BocGTR1c may be the key genes involved in the glucosinolate accumulation in different tissues of Chinese kale.

5.
Molecules ; 24(3)2019 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-30691151

RESUMO

Homogeneous liquid⁻liquid extraction (HLLE) has attracted considerable interest in the sample preparation of multi-analyte analysis. In this study, HLLEs of multiple phenolic compounds in propolis, a polyphenol-enriched resinous substance collected by honeybees, were performed for improving the understanding of the differences in partition efficiencies in four acetonitrile⁻water-based HLLE methods, including salting-out assisted liquid⁻liquid extraction (SALLE), sugaring-out assisted liquid⁻liquid extraction (SULLE), hydrophobic-solvent assisted liquid⁻liquid extraction (HSLLE), and subzero-temperature assisted liquid⁻liquid extraction (STLLE). Phenolic compounds were separated in reversed-phase HPLC, and the partition efficiencies in different experimental conditions were evaluated. Results showed that less-polar phenolic compounds (kaempferol and caffeic acid phenethyl ester) were highly efficiently partitioned into the upper acetonitrile (ACN) phase in all four HLLE methods. For more-polar phenolic compounds (caffeic acid, p-coumaric acid, isoferulic acid, dimethoxycinnamic acid, and cinnamic acid), increasing the concentration of ACN in the ACN⁻H2O mixture could dramatically improve the partition efficiency. Moreover, results indicated that NaCl-based SALLE, HSLLE, and STLLE with ACN concentrations of 50:50 (ACN:H2O, v/v) could be used for the selective extraction of low-polarity phenolic compounds. MgSO4-based SALLE in the 50:50 ACN⁻H2O mixture (ACN:H2O, v/v) and the NaCl-based SALLE, SULLE, and STLLE with ACN concentrations of 70:30 (ACN:H2O, v/v) could be used as general extraction methods for multiple phenolic compounds.


Assuntos
Acetonitrilos/química , Extração Líquido-Líquido , Fenóis/química , Fenóis/isolamento & purificação , Própole/química , Água/química , Cromatografia Líquida de Alta Pressão , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Solventes/química , Temperatura Ambiente
6.
Int J Oncol ; 54(3): 845-858, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30628643

RESUMO

Gastric cancer (GC) is one of the most common and fatal types of cancers worldwide and the specific mechanism has not been completely elucidated. microRNA (miR)­3664­5P has rarely been studied and the aim of the present study was to assess an association between miR­3664­5P and GC. Differences in miR­3664­5P expression in 100 GC (0.1846±0.08276) and paired normal tissues (0.4382±0.1595) were detected using reverse transcription­quantitative polymerase chain reaction assays (P<0.001). 5­Ethynyl­2­deoxyuridine, Cell Counting Kit­8, transwell and flow cytometry assays were performed in vitro and the results were further verified using a mouse xenotransplantation and a lung metastasis model in vivo. miR­3664­5P was significantly downregulated in GC tissues when compared with normal tissues and positively associated with the prognosis of patients with GC (P<0.001). Overexpression of miR­3664­5P suppressed and miR­3664­5P knockdown promoted the proliferation and metastasis of GC cells in vitro and in vivo. Following the application of bioinformatic algorithms and luciferase reporter assays, metadherin (MTDH) was confirmed as the target gene of miR­3664­5P. miR­3664­5P reduced MTDH expression and downregulated the nuclear factor (NF)­κB signaling pathway. Rescue experiments demonstrated that suppression of MTDH restored the effect of miR­3664­5P inhibitors on GC cell lines. The results suggested that miR­3664­5P suppressed the proliferation and metastasis of GC cells by attenuating the NF­κB signaling pathway via MTDH targeting. Consequently, miR­3664­5P may have potential to be an independent prognostic factor and biomarker in GC.


Assuntos
Moléculas de Adesão Celular/metabolismo , MicroRNAs/metabolismo , NF-kappa B/metabolismo , Transdução de Sinais , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/fisiopatologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Moléculas de Adesão Celular/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células/genética , Feminino , Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos , MicroRNAs/genética , Pessoa de Meia-Idade , Metástase Neoplásica , Prognóstico , Neoplasias Gástricas/genética , Neoplasias Gástricas/mortalidade , Análise de Sobrevida
7.
J Gastrointest Surg ; 2018 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-30298416

RESUMO

BACKGROUND: Laparoscopic surgery is the main trend method in a variety of surgical fields. Post-operative shoulder pain remains a bothersome issue although many surgical techniques have been applied to minimize it. A simple novel approach to reduce shoulder pain without adverse effects during and after laparoscopic surgery is desired. METHODS: This prospective randomized controlled study was conducted to enroll a total of 140 patients to evaluate the efficacy of low flow rate (1 L/min) for induction followed by high flow rate (10 L/min) for maintaining 12 mmHg pneumoperitoneum (group A, n = 70) during laparoscopic cholecystectomy (LC), compared to the continuous high flow rate group (group B, n = 70) in postoperative shoulder pain and other clinical features. The 10-visual analog scale (VAS) was applied for the severity of shoulder pain and scores were obtained at 1, 6, 12, 24, and 48 h after LC. RESULTS: There was no obvious difference in baseline characteristics as well as operative time, occurrence of bradycardia, or hospital stay between groups. The incidence of shoulder pain was not significantly different (group A 45.7% vs group B 48.6%, p = 0.866). However, the patients in group A with shoulder pain reported significantly less pain scores (p < 0.001) at 12 and 24 h after surgery, compared with those in group B. CONCLUSIONS: Applying the strategy of low flow rate to induce pneumoperitoneum followed by high flow rate to maintain the pressure provides advantages to reduce the severity of shoulder pain for patients who underwent LC and then experienced shoulder pain.

8.
Environ Sci Pollut Res Int ; 25(15): 14521-14531, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29527646

RESUMO

Studying the modes of selenium occurrence in high-Se soils and its behaviors can improve understanding and evaluating its cycling, flux, and balance in geo-ecosystems and its influence on health. In this paper, using a modified sequential chemical extraction technique, seven operationally defined selenium fractions and Se valence distribution were determined about five soils in which paddy was planted (W1, W2, W3, W4, W5) and five soils in which maize was planted (H1, H2, H3, H4, H5) around the selenium-rich core, Ziyang County, Shaanxi Province, China. The results show that selenium fractions in the soils mainly include sulfide/selenide and base-soluble Se, and ligand-exchangeable Se is also high for five soils in which paddy was planted. For water-soluble Se, Se (IV) is main Se valence and almost no Se (VI) was determined about five soils in which paddy was planted, while almost 1:1 of Se (IV) and Se (VI) coexist about five soils in which maize was planted. For exchangeable Se, similar results were found. For the first time, two typical high-Se soils (W1 soil and H1 soil) were chosen to measure the pH-dependent solid-solution distribution of selenite in the pH range 3-9, and the results were explained using LCD (ligand and charge distribution) adsorption modeling. The desorbed selenite concentrations from the two soils are in general underestimated by the model due to a comparable binding affinity of phosphate and selenite on goethite and much lower amount of total selenite than total reactively adsorbed phosphate. The pH dependency of adsorption of selenite added to the soil can be successfully described with the LCD model for W1 soil. Whereas considering the influence of Al-oxides, by lowering selenite adsorption affinity constant K of Se adsorption on goethite by 16 times, the LCD model can describe the adsorption much better. The results can help to understand selenium cycling, flux, and balance in typical high-Se soils.

9.
Chem Pharm Bull (Tokyo) ; 66(2): 178-183, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29386469

RESUMO

Total syntheses of carbazomycins A and B were demonstrated using a ytterbium-catalyzed Diels-Alder reaction with (silyloxyvinyl)indole as a diene. The densely substituted benzene ring of the target compound was successfully constructed by functionalization of a hydrocarbazolone intermediate and subsequent aromatization using N-bromosuccinimide.


Assuntos
Carbazóis/síntese química , Catálise , Reação de Cicloadição , Itérbio/química
10.
Exp Clin Transplant ; 16(2): 222-226, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26742858

RESUMO

Antibody-mediated rejection after liver transplant, especially when the donor is not a direct relative; it is associated with additional inconvenience for patients. We encountered a case in which antibody-mediated rejection because of de novo donor specific antibodies against donor human leukocyte antigen developed 6 months after ABO-compatible living-donor liver transplant and was treated with retransplant. A 38-year-old man with hepatitis B virus-related hepatocellular carcinoma underwent living-donor liver transplant with a graft from his wife. Six months later, he experienced fatigue and jaundice. Liver biopsy revealed C4d deposits, and histologic examination showed an antibody-mediated rejection pattern. We re-evaluated recipient-donor human leukocyte antigen matching and tested the patient's blood for antihuman leukocyte antigen donor-specific antibodies against donor human leukocyte antigen. De novo auto-antibodies against human leukocyte antigen-DQ6 were identified by Luminex single antigen beads.Because exhausting all treatment options, a rescue second living-donor liver transplant was planned with the patient's stepdaughter as the donor. Pretransplant human leukocyte antigen matching was performed, and the patient was discharged without event. Two months later, hyperbilirubinemia was noted, and a residual common bile duct from the first donor with chronic fibrosis and stricture was strongly suspected. Redo hepaticojejunostomy was successfully performed, with no problems during 1-years' follow-up. Thus, liver retransplant could be a rescue treatment for antibody-mediated rejection complicated with hepatic failure.


Assuntos
Sistema do Grupo Sanguíneo ABO/imunologia , Autoanticorpos/imunologia , Carcinoma Hepatocelular/cirurgia , Rejeição de Enxerto/cirurgia , Antígenos HLA/imunologia , Histocompatibilidade , Neoplasias Hepáticas/cirurgia , Transplante de Fígado/métodos , Doadores Vivos , Adulto , Biópsia , Carcinoma Hepatocelular/patologia , Carcinoma Hepatocelular/virologia , Colangiopancreatografia Retrógrada Endoscópica , Rejeição de Enxerto/diagnóstico , Rejeição de Enxerto/imunologia , Sobrevivência de Enxerto , Hepatite B/complicações , Teste de Histocompatibilidade , Humanos , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/virologia , Transplante de Fígado/efeitos adversos , Masculino , Reoperação , Resultado do Tratamento
11.
Artigo em Inglês | MEDLINE | ID: mdl-29247927

RESUMO

Homogeneous liquid-liquid extraction (h-LLE) has been receiving considerable attention as a sample preparation method due to its simple and fast partition of compounds with a wide range of polarities. To better understand the differences between the two h-LLE extraction approaches, salting-out assisted liquid-liquid extraction (SALLE) and sugaring-out assisted liquid-liquid extraction (SULLE), have been compared for the partition of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly, and for the co-extraction of proteins. Effects of the amount of phase partition agents and the concentration of acetonitrile (ACN) on the h-LLE were discussed. Results showed that partition efficiency of 10-HDA depends on the phase ratio in both SALLE and SULLE. Though the partition triggered by NaCl and glucose is less efficient than MgSO4 in the 50% (v/v) ACN-water mixture, their extraction yields can be improved to be similar with that in MgSO4 SALLE by increasing the initial concentration of ACN in the ACN-water mixture. The content of co-extracted protein was correlated with water concentration in the obtained upper phase. MgSO4 showed the largest protein co-extraction at the low concentration of salt. Glucose exhibited a large protein co-extraction in the high phase ratio condition. Furthermore, NaCl with high initial ACN concentration is recommended because it produced high extraction yield for 10-HDA and the lowest amount of co-extracted protein. These observations would be valuable for the sample preparation of royal jelly.


Assuntos
Ácidos Graxos Monoinsaturados/isolamento & purificação , Ácidos Graxos/química , Glucose/química , Extração Líquido-Líquido/métodos , Proteínas/isolamento & purificação , Cloreto de Sódio/química , Acetonitrilos , Ácidos Graxos Monoinsaturados/análise , Proteínas/análise
12.
Am Surg ; 84(12): 1882-1888, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30606343

RESUMO

Routine use of intraoperative neuromonitoring (IONM) in thyroid cancer surgery is controversial. We aimed to investigate whether it improves the completeness of thyroidectomy and ensures safety. This retrospective study included 380 thyroid cancer patients who underwent thyroidectomy, by one surgeon, between July 2006 and November 2015. Patients were grouped according to the surgeon's adaptation of IONM, as follows: none (period 1; n = 92), early (period 2; n = 141), and late (period 3; n = 147). The operative time and rates of vocal cord palsy were determined. Surgical completeness was assessed by technetium-99m imaging of the thyroid remnant and serum thyroglobulin measurement before ablation. The rate of recurrent laryngeal nerve (RLN) palsy showed a decreasing trend over time. No permanent RLN palsies occurred in nerves not invaded by tumor after routine IONM was introduced. Technetium-99m uptake (periods 1-3, 0.62 vs 0.32 vs 0.20; P < 0.01) and thyroglobulin levels (periods 1 and 2, 37.93 vs 8.98 ng/mL, respectively; P = 0.034; period 3, 9.10 ng/mL) progressively decreased. The mean thyroglobulin level dropped significantly after introduction of routine IONM. We conclude that routine IONM during thyroid cancer surgery improves surgical completeness and might prevent permanent RLN palsy over time.


Assuntos
Monitorização Intraoperatória , Traumatismos do Nervo Laríngeo Recorrente/prevenção & controle , Nervo Laríngeo Recorrente , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia/métodos , Paralisia das Pregas Vocais/prevenção & controle , Adulto , Testes Diagnósticos de Rotina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nervo Laríngeo Recorrente/fisiologia , Traumatismos do Nervo Laríngeo Recorrente/diagnóstico , Traumatismos do Nervo Laríngeo Recorrente/etiologia , Estudos Retrospectivos , Glândula Tireoide/inervação , Glândula Tireoide/cirurgia , Tireoidectomia/efeitos adversos , Paralisia das Pregas Vocais/diagnóstico , Paralisia das Pregas Vocais/etiologia
13.
Bioconjug Chem ; 28(5): 1351-1355, 2017 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-28448116

RESUMO

Targeted and nontargeted biopolymeric nanoparticles with identical hydrodynamic sizes and surface charges were quantitatively examined in terms of the pharmacokinetic and biodistribution differences in detail. In adding cancer cell targeting folate molecules to the surface of the heparin nanocarriers, the amount of drug delivered to the tumor is doubled, and tumor growth inhibition is significantly enhanced. The folate-targeted heparin particles offered similar therapeutic potentials compared to their synthetic long-circulating analogues, thus presenting a viable alternative for drug-delivery vehicle construction using biological polymers, which are easier for the body to eliminate.


Assuntos
Carcinoma de Células Escamosas/tratamento farmacológico , Cisplatino/farmacologia , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Ácido Fólico/química , Nanopartículas/química , Polímeros/química , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Carcinoma de Células Escamosas/patologia , Cisplatino/química , Heparina/química , Humanos , Camundongos , Camundongos Nus , Nanopartículas/administração & dosagem , Distribuição Tecidual
14.
Talanta ; 167: 385-391, 2017 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-28340735

RESUMO

Herein, via one-step pyrolysis of glucose, dicyandiamide (DCDA) and Fe containing metal-organic framework (Fe-MOF), small Fe3C nanoparticles were in-situ decorated in 3D porous network of N-rich graphene (NGr). The Fe-MOF served as size regulating precursor, layered g-C3N4 (derived from pyrolysis of DCDA) acted as not only a template to guide the growth of small Fe3C nanoparticles, but also the carbon source for 3D porous NGr network. The intrinsic peroxidase-like catalytic activity of Fe3C/NGr was unpredictably discovered, by taking the oxidation reaction of 3,3',5,5'-tetramethylbenzidine (TMB) with H2O2 as a protocol. Fast and distinguished color change, improved stability in exposure to extreme H2O2 concentration and high temperature were obtained. The Michaelis-Menten kinetics was investigated. The detection of glucose was accomplished over a wide concentration range of 2.0~500.0µM with a detection limit lower than most of other similar systems. The reliability of the present sensor was further evaluated by practical monitoring glucose in diluted serum samples. Low cost and simple preparation, fast and distinguished color change, high tolerance to extreme H2O2 concentration and high temperature, endow Fe3C/NGr as one of the promising materials for fast visual colorimetry.


Assuntos
Compostos Inorgânicos de Carbono/química , Colorimetria/instrumentação , Grafite/química , Compostos de Ferro/química , Nanopartículas/química , Nitrogênio/química , Materiais Biomiméticos/química , Glucose/análise , Peróxido de Hidrogênio/análise , Peroxidase/metabolismo , Porosidade
15.
Life Sci ; 175: 1-10, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-28286226

RESUMO

AIMS: Sigma-1 receptor (Sig-1R) is a ligand-regulated endoplasmic reticulum (ER) chaperone involved in cardiac hypertrophy, but it is not known whether Sig-1R is regulated by microRNAs (miRNAs). According to bioinformatic analysis, miR-297 was suggested as a potential target miRNA for Sig-1R. Therefore, we verified whether miR-297 could target Sig-1R and investigated the possible mechanisms underlying the role of miR-297 in cardiac hypertrophy. MAIN METHODS: Bioinformatic analysis combined with laboratory experiments, including quantitative RT-PCR, Western blotting, and luciferase assay, were performed to identify the target miRNA of Sig-1R. Transverse aortic constriction (TAC) model and neonatal rat cardiomyocytes (NCMs) stimulated with angiotensin II (AngII) were used to explore the relationship between miR-297 and Sig-1R. Additionally, the function of miR-297 in cardiomyocyte hypertrophy and ER stress/unfolded protein response (UPR) signaling pathway was investigated by transfecting miR-297 mimics/inhibitor. KEY FINDINGS: miR-297 levels were increased in both TAC-induced hypertrophic heart tissue and AngII-induced cardiomyocyte hypertrophy. Up-regulation of miR-297 by specific mimics exacerbated AngII-induced cardiomyocyte hypertrophy, whereas inhibition of miR-297 suppressed the process. During cardiomyocyte hypertrophy, Sig-1R expression, which was negatively regulated by miR-297 by directly targeting its 3'untranslated region (UTR), was decreased. Furthermore, attenuation of miR-297 inhibited the activation of X-box binding protein 1 (Xbp1) and activating transcriptional factor 4 (ATF4) signaling pathways in NCMs. SIGNIFICANCE: Our data demonstrate that miR-297 promotes cardiomyocyte hypertrophy by inhibiting the expression of Sig-1R and activation of ER stress signaling, which provides a novel interpretation for cardiac hypertrophy.


Assuntos
Cardiomegalia/metabolismo , Estresse do Retículo Endoplasmático , MicroRNAs/metabolismo , Miócitos Cardíacos/metabolismo , Receptores sigma/biossíntese , Transdução de Sinais , Resposta a Proteínas não Dobradas , Animais , Cardiomegalia/patologia , Células Cultivadas , Biologia Computacional , Modelos Animais de Doenças , Masculino , Miócitos Cardíacos/patologia , Ratos , Ratos Sprague-Dawley
16.
Biosens Bioelectron ; 92: 646-653, 2017 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-27836614

RESUMO

For the purpose of effectively utilizing the visible light, photoelectrochemical (PEC) detection represents a unique detection signal and different energy form of the excitation source. In this work, ultrathin MoS2 nanosheets with narrow band gap were successfully fabricated by a facile C3N4 sacrificial template assisted thermolytical approach. Upon immobilizing glucose oxidase, excellent photocatalytic activity towards glucose is achieved in neutral buffer solution. As a novel visible light sensitive photocatalytic material, ultrathin MoS2 nanosheets present a detection limit of ~0.61nM, which is much lower than those with the similar configurations reported previously. Based on the excellent anti-interference property, the feasibility of applying the proposed sensor to determine glucose in human serum is further demonstrated. This work provides new insight into the fabrication of promising visible light sensitive two-dimensional layered transition-metal chalcogenides nanostructures for construction of photoelectrochemical biosensors.


Assuntos
Glicemia/análise , Dissulfetos/química , Técnicas Eletroquímicas/métodos , Molibdênio/química , Nanoestruturas/química , Técnicas Biossensoriais/métodos , Humanos , Luz , Limite de Detecção , Nanoestruturas/ultraestrutura , Processos Fotoquímicos
17.
Cell Cycle ; 16(4): 348-359, 2017 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-27929749

RESUMO

NADPH oxidase 4 (NOX4) and the NOX4-related redox signaling are implicated in cardiac hypertrophy. NOX4 is interrelated with endoplasmic reticulum stress (ERS). Spliced X-box binding protein 1 (Xbp1s) is a key mediator of ERS while its role in cardiac hypertrophy is still poorly understood. Recently, receptor interacting protein kinase 1(RIPK1) has been increasingly reported to be associated with ERS. Therefore, we aimed to test the hypothesis that Xbp1s mediates NOX4-triggered cardiac hypertrophy via RIPK1 signaling. In the heart tissue of transverse aortic constriction (TAC) rats and in primary cultured neonatal cardiomyocytes(NCMs) treated with angiotensinII(AngII) or isoproterenol (ISO), NOX4 expression and reactive oxygen species (ROS) generation, and expression of Xbp1s as well as RIPK1-related phosphorylation of P65 subunit of NF-κB were elevated. Gene silencing of NOX4 by specific small interfering RNA (siRNA) significantly blocked the upregulation of NOX4, generation of ROS, splicing of Xbp1 and activation of the RIPK1-related NF-κB signaling, meanwhile attenuated cardiomyocyte hypertrophy. In addition, ROS scavenger (N-acetyl-L-cysteine, NAC) and NOX4 inhibitor GKT137831 reduced ROS generation and alleviated activation of Xbp1 and RIPK1-related NF-κB signaling. Furthermore, splicing of Xbp1 was responsible for the increase in RIPK1 expression in AngII or ISO-treated NCMs. Upregulated RIPK1 in turn activates NF-κB signaling in a kinase activity-independent manner. These findings suggest that Xbp1s plays an important role in NOX4-triggered cardiomyocyte hypertrophy via activating its downstream effector RIPK1, which may prove significant for the development of future therapeutic strategies.


Assuntos
Cardiomegalia/metabolismo , NADPH Oxidases/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Proteína 1 de Ligação a X-Box/metabolismo , Animais , Inativação Gênica , Modelos Biológicos , Miocárdio/metabolismo , Miocárdio/patologia , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/patologia , NADPH Oxidase 4 , NF-kappa B/metabolismo , RNA Interferente Pequeno/metabolismo , Ratos Sprague-Dawley , Transdução de Sinais , Regulação para Cima
18.
Nanoscale ; 8(36): 16251-16258, 2016 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-27714037

RESUMO

The hydrogen evolution reaction (HER) from water through electrocatalysis using highly active noble metal-free catalysts as an alternative to precious Pt-based catalysts holds great promise for clean and renewable energy systems. Here, Mo2C nanosheets well-regulated in N-doped carbon (MCNS/NC)are facilely achieved by in situ confining carburization of a Mo-based inorganic-organic lamellar mesostructure at 900 °C, and demonstrated for the first time as an ultra-efficient and durable HER catalyst. The MCNS/NC displays a very low onset overpotential of ∼0 mV and an overpotential of 19 mV at 10 mA cm-2 with a small Tafel slope of 28.9 mV dec-1 in acid media, which is remarkably superior to most other transition metal-based catalysts and comparable to commercial Pt/C. The HER kinetics are further studied by EIS and Tafel slope analysis, suggesting the dominant Volmer-Tafel mechanism. An "outside-in" carburization mechanism for the evolution of MCNS/NC is proposed through detailed investigation of the samples annealed at different temperatures. This study highlights the importance of designing well-regulated functional nanostructures combined with a conductive carbonaceous matrix for versatile applications.

19.
Sci Rep ; 6: 33007, 2016 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-27623342

RESUMO

It is suspected that some neurodegenerative diseases are a result of the disturbance of copper (Cu) homeostasis, although it remains unclear whether the disturbance of Cu homeostasis has aberrant effects on neurons. Herein, we investigated Cu metabolism specifically in neurons in terms of changes in the intracellular Cu concentration and the expression of Cu-regulating genes, such as Cu transporters and metallothioneins (MTs), before and after the differentiation of rat pheochromocytoma cells (PC12 cells) into neurons. After the differentiation, Cu and Zn imaging with fluorescent probes revealed an increase in intracellular Cu concentration. The concentrations of other essential metals, which were determined by an inductively coupled plasma mass spectrometer, were not altered. The mRNA expression of the Cu influx transporter, Ctr1, was decreased after the differentiation, and the differentiated cells acquired tolerance to Cu and cisplatin, another substrate of Ctr1. In addition, the expression of MT-3, a brain-specific isoform, was increased, contrary to the decreased expression of MT-1 and MT-2. Taken together, the differentiation of PC12 cells into neurons induced MT-3 expression, thereby resulting in intracellular Cu accumulation. The decrease in Ctr1 expression was assumed to be a response aimed at abolishing the physiological accumulation of Cu after the differentiation.


Assuntos
Cobre/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Animais , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Diferenciação Celular , Expressão Gênica , Homeostase , Metalotioneína/genética , Metalotioneína/metabolismo , Metais/toxicidade , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Neurônios/efeitos dos fármacos , Células PC12 , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Zinco/metabolismo
20.
Biochem Biophys Res Commun ; 478(4): 1582-7, 2016 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-27586273

RESUMO

The present study aimed to investigate miR-590-3p's role in osteogenic differentiation of human mesenchymal stem cells (hMSC). HMSC were cultured and transfected with empty vector, miR-590-3p vector to construct control hMSC and miR-590-3p overexpression hMSC. MiR-590-3p suppressed hMSC was created with oligonucleotide transient transfection. All 3 groups of cells were analyzed for their osteogenic level as well as Wnt/ß-catenin signaling pathway activities. The results of alizarin red staining indicated that overexpression of miR-590-3p significantly enhanced mineralized deposition. Osteogenic markers including ALP, OC and SOST were also up-regulated. The result of dual-luciferase and western blot analysis indicated that miR-590-3p bound to 3'UTR of APC mRNA selectively. By suppressing mRNA level of APC, over-expressed miR-590-3p stabilized ß-catenin and increased the transcription activities of downstream target genes. These result suggested that miR-590-3p can promote osteogenic differentiation via suppressing APC expression and stabilizing ß-catenin.


Assuntos
Diferenciação Celular/genética , Regulação da Expressão Gênica , Genes APC , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , MicroRNAs/metabolismo , Osteogênese/genética , Sequência de Bases , Western Blotting , Humanos , Luciferases/metabolismo , MicroRNAs/genética , Via de Sinalização Wnt/genética
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