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1.
Artigo em Inglês | MEDLINE | ID: mdl-31930545

RESUMO

BACKGROUND: Catheter ablation (CA) is a promising option in most patients with refractory atrial fibrillation (AF). However, data on over 5 years' outcomes with larger numbers in hypertrophic cardiomyopathy (HCM) patients with AF have not been reported. We assessed the outcome of 120 HCM patients following CA compared with a non-CA group and general patients without AF matched by HCM type with a 61.9 ± 31.6-month follow-up. METHODS AND RESULTS: A total of 120 patients (age 61 ± 9.8 years, female n = 43, 35.8%) with paroxysmal AF (n = 60, 50%) and persistent AF (n = 60, 50%) were enrolled. Of the 120 patients, 48 (40%) required redo procedures, and 82 (68.3%) were in sinus rhythm at the last evaluation. The composite clinical events rate following the initial CA was lower than that in the non-CA group (P = .023) and was also comparable to that in general patients without AF matched by HCM type (P = .729). Female (HR 2.358, 95% CI, 1.151-4.831; P = .019), NYHA functional class III-IV (HR 2.422, 95% CI, 1.032-5.685; P = .042) and left atrial diameter ≥50 mm (HR 3.319, 95% CI, 1.469-7.499; P = .004) were predictors of AF recurrence after multiple procedures. CONCLUSIONS: CA was successful in restoring long-term sinus rhythm and improving symptomatic status in most HCM patients with refractory AF especially for those patients with small atrial size and mild symptoms. In addition, CA may contribute to the prevention of major clinical adverse events in the long-term clinical course.

2.
J Viral Hepat ; 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31981279

RESUMO

Noninvasive tests (NITs) for liver fibrosis are highly needed for chronic hepatitis B (CHB) patients. We aimed to investigate whether plateletcrit (PCT) could be used as a NIT in predicting liver fibrosis for CHB patients. Five hundred and sixty-seven treatment-naïve CHB patients with available liver biopsies were included. Patients were randomly divided into a derivation cohort (n = 378) and a validation cohort (n = 189). The diagnostic accuracy of PCT was evaluated using receiver operating characteristic (ROC) curves. In the derivation cohort, PCT in CHB patients with S2-S4 (0.14%), S3-S4 (0.13%) and S4 (0.12%) was lower than patients with S0-S1 (0.17%, P < .001), S0-S2 (0.17%, P < .001) and S0-S3 (0.16%, P < .001), respectively. PCT was an independent predictor of significant fibrosis (≥S2), advanced fibrosis (≥S3) and cirrhosis (S4). The area under the ROC curve (AUROC) of PCT in predicting significant fibrosis, advanced fibrosis and cirrhosis was 0.645, 0.709 and 0.714, respectively. The AUROC of PCT was higher than the aspartate transaminase to platelet ratio index (APRI) in identifying advanced fibrosis and cirrhosis, while this was comparable with APRI in identifying significant fibrosis. The diagnostic value of PCT was comparable with fibrosis-4 score (FIB-4) in predicting significant fibrosis, advanced fibrosis and cirrhosis. In the validation cohort, PCT could also identify significant fibrosis, advanced fibrosis and cirrhosis with similar diagnostic accuracy as in the derivation cohort. PCT represents a simple and inexpensive indictor for liver fibrosis in CHB patients. PCT is just as good or better than other more complex tools for staging liver fibrosis in CHB patients.

3.
Sci Total Environ ; 708: 134572, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31796280

RESUMO

The Pearl River Basin is a region with elevated geochemical baseline levels due to high metals/metalloids content in the sediments and soils. In this paper we systematically analyzed the behavior of these metals/metalloid (Cr, Ni, Cu, Zn, Cd, As, and Pb) in bedrock, riverbed sediments, soils, and river water in three different mono-lithological areas (carbonate, basalt, and mud-shale). The results show that the content of transition elements (Cr, Ni, Cu, and Zn) in carbonate rocks are much lower than for shales and basalts, but these metals produce higher enrichment levels in sediments and soils through rapid weathering. Furthermore, Cd, As and Pb are significantly enriched in the upper soils of the carbonate profile and the watersheds dispersed with black shales have distinctly higher Cd enrichment levels. This secondary enrichment of metals through the weathering and pedogenesis of carbonate rocks, and the discharge of metal elements by the weathering of the black rock series, leads to metal pollution in the Pearl River. Most of the small watersheds in the upper reaches of the Pearl River exhibit low or moderate ecological risk but considerable ecological risk exist in the watersheds dispersed with these black shales. Among all the trace elements, Cd generate the highest ecological risk in the individual small watersheds in the upper reaches of the Pearl River.

4.
Mikrochim Acta ; 186(12): 855, 2019 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784817

RESUMO

Molybdenum oxide quantum dots (MoOx QDs) were synthesized by a one-pot method and used as a versatile probe in an electrochemiluminescent (ECL) immunoassay of the non-small cell lung cancer biomarker cytokeratin 19 fragment 21-1 (CYFRA21-1) as a model analyte. The MoOx QDs exhibited stable and strong cathodic green ECL, with an emission peak at 535 nm, in the presence of K2S2O8 within the potential range of -2.0 to 0 V. On exposure to CYFRA21-1, the ECL decreases because of the immunoreaction between CYFRA21-1 and its antibody which generates a barrier for electron transfer. The determination of CYFRA21-1 with favorable analytical performances was successfully realized under the optimal conditions. ECL decreases linearly in the 1 pg mL-1 to 350 ng mL-1 CYFRA21-1 concentration range, and the detection is as low as 0.3 pg mL-1. Excellent recoveries from CYFRA21-1-spiked human serum indicate that the assay can be operated under physiological conditions. Graphical abstractSchematic representation of the fabrication of molybdenum oxide quantum dots (MoOx QDs) and the electrochemiluminescent (ECL) immunoassay based on the use of the MoOx QDs ECL probe for cytokeratin 19 fragment 21-1 (CYFRA21-1).

5.
J Clin Gastroenterol ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31789771

RESUMO

BACKGROUND: Serum hepatitis B e antigen (HBeAg) status is associated with the progression of chronic hepatitis B (CHB). The authors aimed to investigate the relationship between HBeAg status and liver pathology in CHB patients. METHODS: A total of 683 treatment-naive CHB patients who had undergone liver biopsy were retrospectively enrolled from 2 medical centers. Propensity score-matching (PSM) method was performed to adjust the imbalance of baseline confounders between HBeAg-positive and HBeAg-negative CHB patients. RESULTS: HBeAg-negative CHB patients (n=338) exhibited more advanced liver fibrosis than HBeAg-positive CHB patients (n=345) before PSM (P<0.001). However, there were no significant differences in the distribution of inflammation grades between HBeAg-positive and HBeAg-negative CHB patients (P=0.051). Of these 683 CHB patients, 123 patients were included in each group after PSM. HBeAg-negative CHB patients still showed significantly advanced liver fibrosis as compared with HBeAg-positive CHB patients (P=0.03) after PSM. Furthermore, the distribution of liver inflammation grades in the HBeAg-negative CHB patients was also more severe than patients with HBeAg-positive (P=0.037). HBeAg-negative status was identified as an independent risk factor of significant liver fibrosis (P=0.011) by multivariate analysis. CONCLUSIONS: HBeAg negativity is associated with more advanced liver fibrosis in CHB patients.

6.
Hepatology ; 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31858599

RESUMO

First, although the investigators had adjusted for some potentially beneficial medications of HCC prevention including antiviral therapy and antiplatelet therapy, they failed to adjust for the concomitant use of antidiabetic medications(1) . We noted that 39.6% of patients in the statin cohort and 10.9% of patients in the non-statin cohort had diabetes mellitus in the study(1) . Antidiabetic medications are proved to have inherent cancer-modifying effects on HCC and may bias the results(2) . Moreover, coffee consumption is also demonstrated to reduce the HCC risk and should be adjusted(3) .

7.
Artigo em Inglês | MEDLINE | ID: mdl-31691480

RESUMO

Ligand-protected gold nanoclusters (AuNCs) have emerged as a new class of electrochemiluminescence (ECL) luminophores for their interesting catalytic and emission properties, although their quantum yield (ΦECL ) in aqueous medium is low with a poor mechanistic understanding of the ECL process. Now it is shown that drying AuNCs on electrodes enabled both enhanced electrochemical excitation by an electrocatalytic effect, and enhanced emission by aggregation-induced ECL (AIECL) for 6-aza-2-thiothymine (ATT) protected AuNCs with triethylamine (TEA) as a coreactant. The dried ATT-AuNCs/TEA system resulted in highly stable visual ECL with a ΦECL of 78 %, and a similar enhancement was also achieved with methionine-capped AuNCs. The drying enabled dual-enhancement mechanism has solved a challenging mechanistic problem for AuNC ECL probes, and can guide further rational design of ECL emitters.

8.
Heart Rhythm ; 16(12): 1783-1790, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31513945

RESUMO

BACKGROUND: Cardiac resynchronization therapy (CRT) via biventricular pacing has demonstrated clinical benefits in patients with heart failure (HF) and ventricular dyssynchrony. Other approaches of CRT is little known. OBJECTIVE: The purpose of this study was to assess the feasibility, safety, and efficacy of left bundle branch area pacing (LBBAP) in patients with HF and left bundle branch block (LBBB). METHODS: Eleven consecutive patients with HF, reduced left ventricular ejection fraction and LBBB and indicated for CRT were recruited. LBBAP was achieved via transventricular septal approach and characterized by narrower QRS duration, shortened peak left ventricular activation time, and right bundle branch conduction delay on the electrocardiogram. Electrocardiogram, echocardiogram, and cardiac function were evaluated at baseline and follow-up. Interventricular mechanical delay and 3-dimensional tissue synchronization imaging during LBBAP and intrinsic LBBB status were measured by echocardiography at follow-up. RESULTS: LBBAP significantly shortened QRS duration (from baseline 180.00 ± 15.86 ms to 129.09 ± 15.94 ms; P < .01) and left ventricular activation time (from baseline 108.18 ± 15.54 ms to 80.91 ± 9.95 ms; P < .01). Interventricular mechanical delay and the standard deviation of tissue synchronization imaging of 12 left ventricular (LV) segments were significantly shorter during LBBAP than in intrinsic LBBB status (both with P < .01). At a mean follow-up period of 6.7 months, New York Heart Association functional class, plasma level of B-type natriuretic peptide, LV end-systolic diameter, and left ventricular ejection fraction were significantly improved (all with P < .05 vs baseline). CONCLUSION: The study demonstrates that LBBAP is clinically feasible in patients with systolic HF and LBBB. LBBAP can be a new CRT technique to correct LBBB, provide ventricular synchrony, and improve clinical symptoms with LV reverse remodeling.

9.
Emerg Microbes Infect ; 8(1): 1157-1167, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31373538

RESUMO

The spread of influenza A/H3N2 variants possessing the hemagglutinin 121 K mutation and the unexpectedly high incidence of influenza in the 2017-2018 northern hemisphere influenza season have raised serious concerns about the next pandemic. We summarized the national surveillance data of seasonal influenza in China and identified marked differences in influenza epidemics between northern and southern China, particularly the predominating subtype and the presence of an additional summer peak in southern China. Notably, a minor spring peak of influenza caused by a different virus subtype was also observed. We also revealed that the 3C.2a lineage was dominant from the summer of 2015 to the end of the 2015-2016 peak season in China, after which the 3C.2a2 lineage predominated despite the importation and co-circulation of the 121 K variants of 3C.2a1 and 3C.2a3 lineages at the global level. Finally, an analysis based on genetic distances revealed a delay in A/H3N2 vaccine strain update. Overall, our results highlight the complicated circulation pattern of seasonal influenza in China and the necessity for a timely vaccine strain update worldwide.


Assuntos
Variação Genética , Vírus da Influenza A Subtipo H3N2/classificação , Vírus da Influenza A Subtipo H3N2/genética , Influenza Humana/epidemiologia , Influenza Humana/virologia , China/epidemiologia , Humanos , Incidência , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Epidemiologia Molecular , Filogeografia
10.
J Integr Plant Biol ; 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31342638

RESUMO

Nitrate is one of the main inorganic nitrogen sources for plants. Nitrate absorption from soils is achieved through the combined activities of specific nitrate transporters. Nitrate transporter 2.1 (NRT2.1) is the major component of the root high-affinity nitrate transport system in Arabidopsis thaliana. Studies to date have mainly focused on transcriptional control of NRT2.1. Here, we show that NRT2.1 protein stability is also regulated in response to nitrogen nutrition availability. When seedlings were transferred to nitrate-limited conditions, the apparent half-life of NRT2.1 in roots increased from 3 to 9 h. This stabilization of NRT2.1 protein occurred rapidly, even prior to the transcriptional stimulation of NRT2.1. Furthermore, we revealed that phosphorylation at serine 28 (Ser28) of NRT2.1 is involved in regulating the stability of this protein. Substitution of Ser28 by alanine resulted in unstable NRT2.1, and this loss-of-phosphorylation mutant (NRT2.1S28A ) failed to complement the growth-restricted phenotype of the nrt2.1 mutant when a low concentration of nitrate was the sole nitrogen source. These results demonstrate that phosphorylation at Ser28 is crucial for NRT2.1 protein stabilization and accumulation in response to nitrate limitation.

13.
ACS Appl Mater Interfaces ; 11(27): 24812-24819, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31241892

RESUMO

This report outlines a versatile high-performance electrochemiluminescence (ECL) enzyme-linked immunosorbent assay (ELISA) platform, which combines the merits of high-quantum-yield Au nanocluster (AuNC) probe-based ECL technology, the efficient ECL-resonance energy-transfer (ECL-RET) strategy, and highly sensitive and specific ELISA technology. The ECL detection procedure was performed on a recyclable MnO2/AuNC-modified glassy carbon electrode interface by taking advantage of the ECL-RET between the AuNC probe and MnO2 nanomaterials (NMs) to quench the ECL intensity. The etching of MnO2 NMs by the product of ALP-based ELISA recovers the ECL signal. Notably, the ELISA process and the ECL detection procedure in this system are independent. Thus, the ECL-ELISA system can effectively avoid the influence of complex biological samples, and the ECL efficiency of the AuNC probe can be used readily. As demonstrated on TNF-α, because of the abovementioned characteristics, the ECL-ELISA platform presented an extremely wide dynamic range, with a detection limit of 2 orders lower than ELISA. Moreover, the system was also applicable for ultrahigh sensitive detection of various disease-related proteins and able to detect trace biomarkers in real serum samples. Therefore, this multifunctional ECL assay platform is versatile, facile, ultrasensitive, recyclable, and sufficiently straightforward for trace biomarker detection in complex biological samples. This approach not only enriches the foundational study of ECL devices but also greatly expands the potential application of ECL sensors in biological testing and clinical high-throughput diagnosis.

14.
Int J Cardiovasc Imaging ; 35(9): 1671, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31079288

RESUMO

In the original publication of the article, the second author name has been misspelt. The correct name is given in this Correction. The original article has been corrected.

15.
Mikrochim Acta ; 186(5): 301, 2019 04 25.
Artigo em Inglês | MEDLINE | ID: mdl-31028498

RESUMO

It is found that catechol inhibits the oxidase-mimicking activity of chitosan-protected platinum nanoparticles (Chit-PtNPs) by competing with the substrate for the active site of the Ch-PtNPs. The inhibition mechanism of catechol is different from that of ascorbic acid in that it neither reacts with O2•- nor reduces the oxidized 3,3',5,5'-tetramethylbenzidine (TMB). Tyrosinase (TYRase) catalyzes the oxidation of catechol, thus restoring the activity of oxidase-mimicking Chit-PtNPs. By combining the Chit-PtNP, catechol, and TYRase interactions with the oxidation of TMB to form a yellow diamine (maximal absorbance at 450 nm), a colorimetric analytical method was developed for TYRase determination and inhibitor screening. The assay works in the 0.5 to 2.5 U·mL-1 TYRase activity range, and the limit of detection is 0.5 U·mL-1. In our perception, this new assay represents a powerful approach for determination of TYRase activity in biological samples. Graphical abstract Schematic representation of a colorimetric method for tyrosinase (TYRase) detection and inhibitor screening. It is based on the fact that catechol can inhibit the oxidase-like activity of chitosan-stabilized platinum nanoparticles (Ch-PtNPs) by competing with the substrate for the active sites and TYRase can catalyze the oxidation of catechol.

16.
Anal Chim Acta ; 1065: 21-28, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31005147

RESUMO

This report outlines an ultrasensitive electrochemiluminescence (ECL) sensing platform based on water-soluble tungsten oxide quantum dots (WOx QDs) for the detection of dopamine (DA) released from P12 cells. The WOx QDs with good stability and water solubility were prepared by a facile and green hydrothermal method, and used to modify a glassy carbon electrode (WOx QDs/GCE). The proposed ECL sensor exhibited a stable and strong cathodic ECL signal when potassium peroxodisulfate (K2S2O8) as the coreactant in aqueous solution. The possible ECL mechanism was studied and deduced, and the ECL response signal of the proposed sensor decreased rapidly in the presence of dopamine (DA). Under optimal conditions, the ECL signals of WOx QDs linearly decreased with the increase of DA concentration in the range of 10-15 M to 10-9 M and 10-9 M to 10-5 M, respectively. The detection limit was as low as 10-15 M (S/N = 3). Based on these results, this method has been successfully applied to the determination of DA released by PC12 cells. The detection linear range for the detection of DA released by PC12 cells was from 0.1 to 0.9 µM with a detection limit of 0.045 µM. Therefore, the proposed ECL sensor displayed high sensitivity, good specificity and long-term stability, which may shed light on a new way to construct other high-performance ECL detection systems based on WOx or WOx QDs-based nanocomposites.


Assuntos
Técnicas Biossensoriais , Dopamina/análise , Técnicas Eletroquímicas , Medições Luminescentes , Óxidos/química , Pontos Quânticos/química , Tungstênio/química , Animais , Dopamina/metabolismo , Óxidos/síntese química , Células PC12 , Tamanho da Partícula , Ratos , Solubilidade , Propriedades de Superfície , Água/química
17.
Virus Genes ; 55(4): 440-447, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31025287

RESUMO

The untranslated regions within viral segments are the essential promoter elements required for the initiation of viral replication and transcription. The end of the UTR sequence and part of the ORF sequence constitute the packaging signal for progeny viruses. To explore the influence of single-point and multi-site joint mutations in the UTR of the NA gene on the viral expression, we select clones with upregulated expression of the reporter gene and analyze their sequence characteristics. Bioinformatics methods were used to analyze polymorphisms in the untranslated region (UTR) of the neuraminidase gene of the H9N2 influenza A virus. Using the RNA polymerase I reporting system with enhanced green fluorescence protein (EGFP) gene as the reporter gene, libraries containing random mutations at sites within the N2 UTR were constructed using random mutagenesis. The mutants were selected from the randomized mutagenesis libraries for the N2-UTR. The N2-UTR-RNA polymerase I fluorescence reporter system was identified by sequencing and transfected into infected MDCK cells. The expression of the reporter EGFP was observed using fluorescence microscopy, and the relative fluorescence intensity was measured using a multifunctional microplate reader to analyze the expression of the reporter gene (EGFP) qualitatively and quantitatively. Herein, an RNA polymerase reporter system was constructed to rescue the mutated viruses and measure their tissue culture infective dose (TCID50). The results showed that the U13 → C13 mutation in the 3'end of the NA gene promoted the expression of viral RNA and protein, and mutation of other sites within the UTR could differentially regulate viral genomic transcription and translation. These data showed that the U13 → C13 mutation within the variable region of the 3'UTR of the NA gene in the H9N2 influenza virus promotes viral genomic expression and infection.


Assuntos
Vírus da Influenza A Subtipo H9N2/genética , Neuraminidase/genética , Proteínas Virais/genética , Replicação Viral/genética , Regiões 3' não Traduzidas , Animais , Cães , Regulação Viral da Expressão Gênica , Genes Reporter , Proteínas de Fluorescência Verde/genética , Vírus da Influenza A Subtipo H9N2/fisiologia , Células Madin Darby de Rim Canino , Mutagênese , RNA Viral
18.
Int J Cardiovasc Imaging ; 35(6): 1009-1017, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30941563

RESUMO

Right ventricular (RV) impairment after cancer therapy-related cardiotoxicity and its prognostic implications in lung cancer have not been examined. The present research sought to evaluate RV structure, function, and mechanics in stage III non-small-cell lung cancer (NSCLC) before and after concurrent chemoradiotherapy (CCRT), and to explore the associations between RV impairments, radiation dose, and all-cause mortality. This prospective investigation included 128 inoperable NSCLC patients who were scheduled to receive CCRT. Echocardiographic examination and strain evaluation was performed at baseline and 6 months post-CCRT in all participants. Conventional RV dimensions revealed no significant changes post-CCRT. However, a reduction in RV free wall strain (RV-fwLS) was observed at 6 months post-CCRT (- 28.3 ± 4.6% vs. - 25.5 ± 4.8%, P < 0.001). The same was revealed for global RV longitudinal strain (RV-GLS) (- 23.4 ± 2.9% vs. - 20.2 ± 3.4%, P < 0.001). Pearson correlation showed that RV radiation mean dose was related with the percentage change in RV-fwLS (r = 0.303, P = 0.001) and RV-GLS (r = 0.284, P = 0.002). In multivariable analysis, the percentage change in RV-fwLS was an independent predictor of all-cause mortality (HR 1.296, 95% CI 1.202-1.428, P < 0.001). RV longitudinal strain is deteriorated at 6 months post-CCRT. The RV mechanics deterioration was associated with radiation dose and affected the long-term outcome of stage III NSCLC patients treated with CCRT.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Quimiorradioterapia/efeitos adversos , Neoplasias Pulmonares/terapia , Contração Miocárdica/efeitos da radiação , Lesões por Radiação/etiologia , Dosagem Radioterapêutica , Disfunção Ventricular Direita/etiologia , Função Ventricular Direita/efeitos da radiação , Adolescente , Adulto , Idoso , Fenômenos Biomecânicos , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Cardiotoxicidade , Quimiorradioterapia/mortalidade , Ecocardiografia Doppler , Feminino , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Contração Miocárdica/efeitos dos fármacos , Estadiamento de Neoplasias , Estudos Prospectivos , Lesões por Radiação/diagnóstico por imagem , Lesões por Radiação/fisiopatologia , Fatores de Tempo , Resultado do Tratamento , Disfunção Ventricular Direita/diagnóstico por imagem , Disfunção Ventricular Direita/fisiopatologia , Função Ventricular Direita/efeitos dos fármacos , Adulto Jovem
19.
Ticks Tick Borne Dis ; 10(3): 639-646, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30824322

RESUMO

OBJECTIVES: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease that is caused by the SFTS virus (SFTSV) and has a high fatality rate. SFTSV-specific antibody profiles among patients with different clinical outcomes are yet to be described. The nucleocapsid protein (NP) is the most immunogenic viral antigen of the SFTSV. This study, therefore, sought to determine NP-specific antibody responses among SFTS patients with different disease progressions. METHODS: In the present study, 43 patients with confirmed SFTS were enrolled in our cohort, and 9 of them deceased. The clinical presentations and key laboratory parameters associated with SFTS fatality were also recorded. Serum samples from each patient were collected every 2 days during their hospitalization. NP-specific IgM and IgG responses as well as Gn or Gc-specific IgM responses were examined by enzyme-linked immunosorbent assay (ELISA), whereas, the dynamic viral loads of SFTSV RNA were quantified via real-time reverse transcription polymerase chain reaction (RT-PCR). RESULTS: First, 77% of patients generated positive NP-specific IgM antibody responses within two weeks since illness onset, defined as 'N-specific IgM-positive patients', while the rest of the patients were termed as 'N-specific IgM-delayed patients'. Only 17% of the patients generated NP-specific IgG responses. The absence of NP-specific humoral responses was strongly associated with a high risk of fatality and severity of SFTS. IgM-positive patients had significantly lower levels of viral loads, less disturbed coagulopathy, and hepatic and cardiac damage compared to IgM-delayed patients. Moreover, compared to severe or fatal SFTS patients, mild SFTS patients had significantly higher magnitudes of NP-specific IgM responses, but not NP-specific IgG, Gn-specific IgM, or Gc-specific IgM responses. The abundance of NP-specific IgM responses negatively correlated with viral loads, coagulation disturbances, and hepatic injuries among SFTS patients. CONCLUSIONS: Our data highlight distinct humoral profiles of NP-specific IgM responses among SFTS patients with different disease progressions and clinical outcomes.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/imunologia , Progressão da Doença , Imunoglobulina M/sangue , Proteínas do Nucleocapsídeo/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Infecções por Bunyaviridae/complicações , Infecções por Bunyaviridae/mortalidade , China , Estudos de Coortes , Doenças Transmissíveis Emergentes/imunologia , Doenças Transmissíveis Emergentes/virologia , Ensaio de Imunoadsorção Enzimática , Feminino , Febre/etiologia , Humanos , Imunidade Humoral , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Phlebovirus , Prognóstico , Estudos Prospectivos , RNA Viral/genética , Estudos Retrospectivos , Carga Viral
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