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1.
Huan Jing Ke Xue ; 40(11): 4900-4913, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854556

RESUMO

Surface water samples were collected from 20 sampling sites in the main stream and its major tributaries of the Yangtze River from April to May 2017. The concentrations of dissolved trace and major elements were analyzed to determine the spatial variation, source identification, and riverine fluxes using various multivariate statistical techniques, including correlation analysis, principal component analysis (PCA), and cluster analysis (CA) with the goal of determining the influence of natural factors and human activities, including the operation of the Three Gorges Dam on the distribution and loading of major and trace elements in the Yangtze River water environment. Spatial distribution results showed that Cu, Zn, Pb, Cd, and As were the major elements affected by human activities in the Yangtze River, and their concentrations downstream were significantly higher than those in the middle and upper reaches (P<0.05). All elements had fairly high concentration values in both channels of the Yangtze River mainstream in Chongqing city and Hanjiang River in Wuhan city, which were mainly related to the enhanced human activities. However, the low concentrations of multi-elements in the reach of the Yangtze River in Yichang were largely caused by the retention effect of Three Gorges Project on element transport, which decreased the riverine loadings of multi-elements. Principal component analysis (PCA) indicated that Na, Mg, K, Ca, Fe, Mn, Co, Ni, Mo, Cr, and V were mainly associated with the weathering and erosion of various rocks and minerals in the river basin. And Cu, Zn, and Pb were mainly affected by enhanced human activities, such as industrial wastewater, metal smelting, and mineral mining, whereas Cd and As were mainly related to agricultural activities. The spatial distribution of trace and major elements showed that concentrations of some elements in the Yangtze River channels were enhanced by human activities. Generally, the heavy metal pollution in the Yangtze River Basin was lower than that in other rivers of the world. However, the annual fluxes of Cu, Zn, Pb, Cd, and As could have far-reaching ecological effects on the Yangtze River estuary and offshore ecological environment.

2.
Anal Chem ; 91(23): 15123-15129, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31697073

RESUMO

Glutamate (Glu) is a critical neurotransmitter for neuronal communication in the nervous system. In vivo studies have shown that the concentration of Glu is reduced within the brains of those afflicted with Alzheimer's disease (AD), which is also associated with the accumulation of pathogenic amyloid-beta (Aß). However, the effects of Aß peptides on the level of Glu release, as well as how Aß-mediated Glu fluctuation is initiated, remain largely unknown. Here, we fabricated a Glu electrochemical biosensor and in situ quantitatively monitored the release of Glu from a single varicosity of Aß1-42-insulted hippocampal neurons. We found that before the depletion of Glu after 300 min of treatment with Aß1-42, a short-duration (30 min) incubation with Aß1-42 caused a dramatic increase in vesicular Glu release compared to that of a control. Further investigation demonstrated that the density of vesicular glutamate transporter 1 (VGLUT1), which is responsible for transport of Glu into synaptic vesicles, also displayed a significant elevation and then dramatic depletion with the extension of the time of treatment with Aß1-42. These results indicate that at the early stage of AD, Aß1-42 induces excessive Glu release, which may overstimulate the N-methyl-d-aspartic acid (NMDA) receptor, resulting in excitotoxicity and damage to neurons. In this work, the amount of Glu released together with its fluctuations under Aß1-42 oligomers toxicity conditions was monitored for the first time, and such monitoring could provide direct and new insights for current research on Aß1-42-induced abnormalities in neurotransmitter release and neuron functions.

3.
Small ; 15(48): e1901787, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31183973

RESUMO

Mitochondria are believed to be the major source of intracellular reactive oxygen species (ROS). However, in situ, real-time and quantitative monitoring of ROS release from mitochondria that are present in their cytosolic environment remains a great challenge. In this work, a platinized SiC@C nanowire electrode is placed into a single cell for in situ detection of ROS signals from intracellular mitochondria, and antineoplastic agent (paclitaxel) induced ROS production is successfully recorded. Further investigations indicate that complex IV (cytochrome c oxidase, COX) is the principal site for ROS generation, and significantly more ROS are generated from mitochondria in cancer cells than that from normal cells. This work provides an effective approach to directly monitor intracellular mitochondria by nanowire electrodes, and consequently obtains important physiological evidence on antineoplastic agent-induced ROS generation, which will be of great benefit for better understanding of chemotherapy at subcellular levels.

4.
J Clin Neurosci ; 64: 145-149, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30929959

RESUMO

Our objective was to assess the impact of hs-cTnT elevation on functional outcome and mortality in AIS patients with large vessel occlusion (LVO) in the anterior circulation 3 months after ET and explore factors affecting hs-cTnT elevation. A total of 143 consecutive AIS patients with large vessel occlusion (LVO) in the anterior circulation following ET in a single stroke center were enrolled between January 2015 and November 2017. Hs-cTnT was quantitated on admission. Demographic characteristics, clinical data, functional outcome and all-cause mortality were compared between patients with elevated hs-cTnT levels (>14 ng/L) and those with normal hs-cTnT levels (≤14 ng/L). 58/143(40.6%) patients showed elevated hs-cTnT levels before ET. Factors independently associated with hs-cTnT elevation were admission NIHSS score (OR = 1.08, 95% CI 1.01-1.16, p = 0.032), coronary heart disease (OR = 4.89, 95% CI 1.82-13.11, p = 0.002) and congestive heart failure (OR = 4.10, 95% CI 1.07-15.68, p = 0.039). In the univariate analysis, patients with elevated hs-cTnT levels were at significantly higher risk of 3-month poor outcome (p = 0.029) and mortality (p < 0.001) than those with normal hs-cTnT levels. After multivariable analysis, hs-cTnT elevation remained an independent predictor of 3-month mortality (OR = 4.49, 95% CI 1.68-11.98, p = 0.003). In this cohort of AIS patients with LVO in the anterior circulation undergoing ET, hs-cTnT elevation is an independent predictor of 3-month mortality. Admission NIHSS score, coronary heart disease and congestive heart failure are independently associated with elevated hs-cTnT levels.


Assuntos
Biomarcadores/sangue , Acidente Vascular Cerebral/cirurgia , Troponina T/sangue , Idoso , Isquemia Encefálica/sangue , Isquemia Encefálica/mortalidade , Isquemia Encefálica/cirurgia , Estudos de Coortes , Procedimentos Endovasculares/métodos , Procedimentos Endovasculares/mortalidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Acidente Vascular Cerebral/sangue , Acidente Vascular Cerebral/mortalidade , Trombectomia/métodos , Trombectomia/mortalidade
5.
ACS Nano ; 13(3): 2849-2857, 2019 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-30803232

RESUMO

Although anti-PD-1 immunotherapy is widely used to treat melanoma, its efficacy still has to be improved. In this work, we present a therapeutic method that combines immunotherapy and starvation therapy to achieve better antitumor efficacy. We designed the CMSN-GOx method, in which mesoporous silica nanoparticles (MSN) are loaded with glucose oxidase (GOx) and then encapsulate the surfaces of cancer cell membranes to realize starvation therapy. By functionalizing the MSN's biomimetic surfaces, we can synthesize nanoparticles that can escape the host immune system and homologous target. These attributes enable the nanoparticles to have improved cancer targeting ability and enrichment in tumor tissues. Our synthetic CMSN-GOx complex can ablate tumors and induce dendritic cell maturity to stimulate an antitumor immune response. We performed an in vivo analysis of these nanoparticles and determined that our combined therapy CMSN-GOx plus PD-1 exhibits a better antitumor therapeutic effect than therapies using CMSN-GOx or PD-1 alone. Additionally, we used the positron emission tomography imaging to measuring the level of glucose metabolism in tumor tissues, for which we investigate the effect with the cancer therapy in vivo.

6.
Cardiovasc Intervent Radiol ; 42(1): 87-94, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29740688

RESUMO

PURPOSE: The present study compared the safety and efficacy of oxycodone with those of fentanyl under non-intubated general anaesthesia in percutaneous microwave ablation (MWA) of a liver tumour abutting the capsule. MATERIALS AND METHODS: Thirty-eight patients underwent MWA of liver cancers abutting the capsule. Patients received 0.1 mg/kg oxycodone (O group) or 1 µg/kg fentanyl (F group) prior to the start of ablation. Both groups received continuous infusions of propofol for non-intubated general anaesthesia during ablation. The primary outcomes were the pain scores (11-point numeric rating scale, NRS) within 24 h after MWA. Vital signs, body movement during ablation, and opioid side effects after ablation were recorded. The need for additional analgesics was recorded 24 h after MWA. RESULTS: The pain NRS scores were lower in the O group than in the F group at 0.5 (P = 0.035), 3 (P = 0.002), and 6 h (P = 0.001) after MWA, and fewer patients required additional analgesics in the O group (6 of 20 vs. 13 of 18, P = 0.022) within 24 h. The average 24-h dose of dezocine was 5.5 ± 4.1 mg in the F group and 2.1 ± 3.3 mg in the O group (P = 0.008). A significant reduction in the respiratory rate (P = 0.020) and more body movements were observed in the F group (P = 0.027) during ablation with non-intubated general anaesthesia. No differences in post-operative nausea and vomiting (PONV) were observed between the two groups, but dizziness occurred significantly more often in the O group (P = 0.033). No significant differences in other vital signs were observed before, during, and after the procedure. CONCLUSIONS: Oxycodone provides better analgesia and reduces post-operative opioid consumption without significant respiratory or hemodynamic instability.


Assuntos
Técnicas de Ablação/métodos , Analgésicos Opioides/uso terapêutico , Fentanila/uso terapêutico , Neoplasias Hepáticas/cirurgia , Oxicodona/uso terapêutico , Dor Pós-Operatória/tratamento farmacológico , Feminino , Humanos , Fígado/cirurgia , Masculino , Micro-Ondas , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento
7.
Nanoscale ; 10(13): 6014-6023, 2018 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-29542756

RESUMO

Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively. The resulting engineered RBCs rapidly adhered to CTCs and the obtained CTC-RBC conjugates were isolated in a magnetic field. After treatment with RBC lysis buffer and centrifugation, CTCs were released and captured. The duration of the entire process was less than three hours. Cell counting showed that the capture efficiency was above 90% and the purity of the obtained CTCs was higher than 75%. The performance of the proposed method exceeded that of MACS® beads (80% for capture efficiency and 20% for purity) under the same conditions. The obtained CTCs could be successfully re-cultured and proliferated in vitro. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.


Assuntos
Eritrócitos/citologia , Ácido Fólico/química , Nanopartículas de Magnetita , Células Neoplásicas Circulantes , Adesão Celular , Linhagem Celular Tumoral , Separação Celular , Molécula de Adesão da Célula Epitelial , Humanos
8.
Nanotechnology ; 29(8): 084002, 2018 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-29339567

RESUMO

Recently, red blood cell (RBC) membrane-coated nanoparticles have attracted much attention because of their excellent immune escapability; meanwhile, gold nanocages (AuNs) have been extensively used for cancer therapy due to their photothermal effect and drug delivery capability. The combination of the RBC membrane coating and AuNs may provide an effective approach for targeted cancer therapy. However, few reports have shown the utilization of combining these two technologies. Here, we design erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy. First, anti-EpCam antibodies were used to modify the RBC membranes to target 4T1 cancer cells. Second, the antitumor drug paclitaxel (PTX) was encapsulated into AuNs. Then, the AuNs were coated with the modified RBC membranes. These new nanoparticles were termed EpCam-RPAuNs. We characterized the capability of the EpCam-RPAuNs for selective tumor targeting via exposure to near-infrared irradiation. The experimental results demonstrate that EpCam-RPAuNs can effectively generate hyperthermia and precisely deliver the antitumor drug PTX to targeted cells. We also validated the biocompatibility of the EpCam-RAuNs in vitro. By combining the molecularly modified targeting RBC membrane and AuNs, our approach provides a new way to design biomimetic nanoparticles to enhance the surface functionality of nanoparticles. We believe that EpCam-RPAuNs can be potentially applied for cancer diagnoses and therapies.

9.
Abdom Radiol (NY) ; 42(6): 1781-1787, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28180923

RESUMO

PURPOSE: To compare the technical success and accuracy of hepatic microwave ablation (MWA) using non-enhanced and enhanced T1-weighted imaging early after ablation. Patients were evaluated with regard to the ablation zone and local tumor progression (LTP). METHODS: This retrospective study conducted between September 2014 and December 2015 which consisted of 56 patients with 56 hepatic malignant lesions who underwent percutaneous MWA. Non-enhanced and contrast-enhanced T1-weighted imagings were performed within 2 days after tumor ablation. The efficacy of ablation assessed according to the hyperintense middle zone on non-enhanced T1-weighted images and the non-enhanced area on contrast-enhanced T1-weighted images were compared. The development of LTP during ≥7 months of follow-up served as the end point. RESULTS: On the non-enhanced T1-weighted images, the ablated region had a characteristic two-zone structure featuring a hyperintense middle zone and a surrounding hypointense band. Among the 56 patients, LTP developed in ten including seven lesions, in which both the non-enhanced T1-weighted and portal-phase images showed incomplete tumor ablation. In two of the remaining three patients, incomplete tumor ablation was detected on the non-enhanced T1-weighted images, whereas the corresponding portal-phase images showed complete ablation. In the remaining patient, no residual tumor was detected on either the non-enhanced T1-weighted or the portal-phase images. In the 46 patients without LTP, there was no evidence of residual tumor on the non-enhanced T1-weighted or portal-phase images obtained early after ablation. CONCLUSIONS: Non-enhanced T1-weighted images are useful in assessing the therapeutic efficacy of MWA of liver tumors early after the procedure.


Assuntos
Ablação por Cateter/métodos , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/cirurgia , Imagem por Ressonância Magnética/métodos , Micro-Ondas/uso terapêutico , Adulto , Idoso , Idoso de 80 Anos ou mais , Meios de Contraste , Progressão da Doença , Gadolínio DTPA , Humanos , Aumento da Imagem/métodos , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Necrose , Estudos Retrospectivos , Sensibilidade e Especificidade , Tomografia Computadorizada por Raios X
10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 37(3): 788-93, 2017 Mar.
Artigo em Chinês, Inglês | MEDLINE | ID: mdl-30148576

RESUMO

The impact analysis of different environments on the fluorescence emission spectrum of pesticides is critical in detecting the concentration of pesticides. In this paper, three kinds of pesticides, carbendazim, carbaryl and fuberidazole, were selected as the research objects. Under different environment, such as different pH values and the presence of different common anion or cation, three-dimensional fluorescence spectral emission (EEM) characteristic of pesticides were analyzed. The experimental results showed that the primary fluorescence peaks for three kinds of pesticides were at λex/λem=280/300, 310/340 and 280/335 nm (respectively); Carbendazim and fuberidazole had a secondary peak at 245/305 nm (PeakB) and 250/340 nm (PeakB). We can come to the conclusion that with the change of pH value, the characteristic of fluorescence emission of carbendazim and fuberidazole is similar. We can find that the fluorescence intensities of carbendazim and fuberidazole were enhanced with the declining of the solution acidity or alkalinity and the fluorescence intensity of carbaryl had not changed with the declining of the solution acidity, but it increased with the declining of the solution alkalinity; the fluorescence emission spectra of the three kinds of pesticides had good fluorescence characteristics with the scope of the pH varying from 6.16 to 7.4. Twelve common ions in water (CO2-3,SO2-4,NO-3,Cl-,HPO2-4,HCO-3,Mg2+,Zn2+,NH+4,Na+,Ca2+,K+) had no significant effect on fluorescence emission characteristics of carbendazim and fuberidazole. The fluorescence intensities were seriously influenced by Fe3+ and Cu2+. The results showed that the pesticides fluorescence intensities were decreased with the ion concentration increasing. It was necessary to consider the quenching effects on pesticides of Fe3+ and Cu2+for the analytic results. The obtained results provided the basic research for improving the accuracy of the heterocyclic pesticides measurement in water.


Assuntos
Praguicidas/química , Poluentes Químicos da Água/análise , Carbaril , Concentração de Íons de Hidrogênio , Espectrometria de Fluorescência
11.
Toxicol Res (Camb) ; 6(4): 544-553, 2017 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30090523

RESUMO

Nifurtimox has been an important treatment for trypanosomiasis for many years, but new research indicates that the drug may also be an effective therapy for malignant neuroblastoma. However, there have been few published reports evaluating the toxicity of nifurtimox in different species. Therefore, to further understand its toxicity and toxicokinetic profiles, Sprague Dawley rats and beagle dogs were orally administered nifurtimox at 0, 25, 75 and 150 mg kg-1 day-1, and 0, 30, 60 and 120 mg kg-1 day-1, respectively, for 28 days, which was followed by a 28-day recovery period. Significant decreases in the body weight and food consumption were observed in rats given 75 and 150 mg kg-1 day-1, but no significant difference was observed in either body weight or food consumption in dogs. No notable gender difference was observed in the rats in our study. The mean Cmax and AUC0-t increased with the exposure time in rats, and systemic exposure on day 28 was notably higher than that on day 1 for each dosing group. In contrast, in dogs the mean Cmax and AUC0-t increased significantly in the 120 mg kg-1 day-1 group only. Other findings in rats included a dose-dependent increase in total bilirubin and urea, a significant increase in the kidney organ coefficient, a decrease in heart and thymus weights, and a decrease in the weight of testes and epididymides tissue in males administered 75 and 150 mg kg-1 day-1, with dead sperms observed in the epididymides and a loss of necrotic cells. Furthermore, the brains of rats administered 150 mg kg-1 day-1 nifurtimox revealed cerebral tissue softening. In dogs there were no treatment-related changes in organ weights during the dosing period. However, deciduous spermatoblasts were observed in the seminiferous tubules and there was a lack of long sperms in the epididymides. The findings from this study demonstrate inter-species differences in nifurtimox toxicity and toxicokinetics. These results are relevant to the evaluation of the wider clinical applications of this drug.

12.
Sci Rep ; 6: 30675, 2016 07 29.
Artigo em Inglês | MEDLINE | ID: mdl-27471137

RESUMO

The aims of this systematic review were to study the analgesic effect of real acupuncture and to explore whether sham acupuncture (SA) type is related to the estimated effect of real acupuncture for musculoskeletal pain. Five databases were searched. The outcome was pain or disability immediately (≤1 week) following an intervention. Standardized mean differences (SMDs) with 95% confidence intervals were calculated. Meta-regression was used to explore possible sources of heterogeneity. Sixty-three studies (6382 individuals) were included. Eight condition types were included. The pooled effect size was moderate for pain relief (59 trials, 4980 individuals, SMD -0.61, 95% CI -0.76 to -0.47; P < 0.001) and large for disability improvement (31 trials, 4876 individuals, -0.77, -1.05 to -0.49; P < 0.001). In a univariate meta-regression model, sham needle location and/or depth could explain most or all heterogeneities for some conditions (e.g., shoulder pain, low back pain, osteoarthritis, myofascial pain, and fibromyalgia); however, the interactions between subgroups via these covariates were not significant (P < 0.05). Our review provided low-quality evidence that real acupuncture has a moderate effect (approximate 12-point reduction on the 100-mm visual analogue scale) on musculoskeletal pain. SA type did not appear to be related to the estimated effect of real acupuncture.


Assuntos
Acupuntura/métodos , Dor Musculoesquelética/terapia , Ensaios Clínicos Controlados Aleatórios como Assunto , Humanos , Resultado do Tratamento
13.
Int J Clin Exp Med ; 8(4): 5175-85, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26131091

RESUMO

OBJECT: The authors retrospectively analyzed the surgical treatment of adult intrinsic pontine gliomas in their department, and to enhance the understanding of technical strategies to treat this disease. METHODS: 7 patients with intrinsic pontine gliomas were recruited for this study, between January 2011 and June 2013. All patients underwent preoperative MRI and Diffusion Tensor Imaging Fiber Tracking (DTI-FT). In addition, multimodal Intraoperative Neuromonitoring (IOM) and Intraoperative Neuronavigation were also applied during microsurgery. RESULTS: 7 patients with intrinsic pontine gliomas were treated at the West China Hospital of Sichuan University. Mean age, mean duration of symptoms prior to diagnosis, and mean duration of follow-up average time were 38.0 years, 2.0 months, and 23.4 months, respectively. The main presentations were progressive cranial nerve deficits and long tract signs. Total resection was achieved in 3 patients, subtotal resection in 2, and partial resection in 2. Postoperative pathological examination revealed: astrocytoma (WHO II) in 4 cases, anaplastic oligoastrocytoma (AO, WHO III) in one case, and anaplastic astrocytoma (AA, WHO III) in two cases. Postoperative radiotherapy were administered to all patients, and 4 patients with astrocytoma (WHO II) rejected chemotherapy. After 11-39 months of follow-up, patient symptoms were resolved or stable without aggravation except one patient died because of rapidly progressive glioma at 11 months after operation. MRI in other patients showed residual tumor size to be unchanged or without obviously recurrence. CONCLUSION: Combining preoperative MRI with preoperative DTI-FT, surgery can be better assessed and the operation for adult intrinsic pontine gliomas can be maximally and safely resected with the aid of Multimodal IOMs and Intraoperative Navigation during microsurgery.

14.
Artigo em Chinês | MEDLINE | ID: mdl-25902686

RESUMO

Malaria data in 2011-2013 were collected and statistically analyzed using Microsoft Excel 2003 software. A total of 501 malaria cases were reported, and the annual incidence was 0.2510/100,000, 0.2486/100,000, and 0.3223/100,000, in 2011, 2012, and 2013, respectively, with an average of 0.2740/100,000 in Hunan Province. All these cases were imported and mainly reported from Changsha (44.3%, 222/501), Shaoyang (16.6%, 83/501), Huaihua (8.4%, 42/501), and Yiyang (8.0%, 40/501). 97.0% (486/501) of the cases were laboratory confirmed cases, while the other 3.0% (15/501) were clinically diagnosed. Among those lab confirmed, 41.3% (207/501) were vivax malaria cases, 47.9% (240/501) falciparum malaria cases, 1.4% (7/501) ovale malaria cases, 0.8% (4/501) malariae cases, 6.6% (33/501) mixed infection, and 2.0% (10/501) were unclassified cases. Most cases (202/501) occurred among persons aged 40-49 years. These patients were mainly farmers, workers, migrant workers, and cadres. 47.7% (239/501) were from Africa and 50.1% (251/501) from Southeast Asia.


Assuntos
Malária , China , Coinfecção , Humanos , Incidência , Malária Falciparum , Malária Vivax
15.
CNS Neurosci Ther ; 19(7): 494-502, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23575351

RESUMO

AIMS: To study the contribution of epidermal growth factor receptor variant III (EGFRvIII) to glioblastoma multiforme (GBM) stemness and gefitinib resistance. METHODS: CD133(+) and CD133(-) cells were separated from EGFRvIII(+) clinical specimens of three patients with newly diagnosed GBM. Then, RT-PCR was performed to evaluate EGFRvIII and EGFR expression in CD133(+) and CD133(-) cells. The tumorigenicity and stemness of CD133(+) cells was verified by intracranial implantation of 5 × 10(3) cells into immunodeficient NOD/SCID mice. Finally, cells were evaluated for their sensitivity to EGFR tyrosine kinase inhibition by gefitinib. RESULTS: RT-PCR results showed that the sorted CD133(+) cells expressed EGFRvIII exclusively, while the CD133(-) cells expressed both EGFRvIII and EGFR. At 6-8 weeks postimplantation, CD133(+) /EGFRvIII(+) /EGFR(-) cells formed intracranial tumors. Cell counting kit-8 results showed that the IC50 values of the three isolated EGFRvIII(+) cell lines treated with gefitinib were 14.44, 16.00, and 14.66 µM, respectively, whereas the IC50 value of an isolated EGFRvIII(-) cell line was 8.57 µM. CONCLUSIONS: EGFRvIII contributes to the stemness of cancer stem cells through coexpression with CD133 in GBMs. Furthermore, CD133(+) /EGFRvIII(+) /EGFR(-) cells have the ability to initiate tumor formation and may contribute to gefitinib resistance.


Assuntos
Antígenos CD/metabolismo , Antineoplásicos/farmacologia , Receptores ErbB/metabolismo , Glicoproteínas/metabolismo , Peptídeos/metabolismo , Quinazolinas/farmacologia , Células-Tronco/efeitos dos fármacos , Células-Tronco/fisiologia , Antígeno AC133 , Adolescente , Adulto , Animais , Neoplasias Encefálicas/patologia , Resistência a Medicamentos , Resistencia a Medicamentos Antineoplásicos , Receptores ErbB/antagonistas & inibidores , Feminino , Citometria de Fluxo , Imunofluorescência , Gefitinibe , Glioblastoma/patologia , Humanos , Imuno-Histoquímica , Separação Imunomagnética , Masculino , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Pessoa de Meia-Idade , Transplante de Neoplasias , Reação em Cadeia da Polimerase em Tempo Real , Adulto Jovem
16.
Comput Math Methods Med ; 2013: 989307, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23533546

RESUMO

Kawasaki disease (KD) is a complex disease, leading to the damage of multisystems. The pathogen that triggers this sophisticated disease is still unknown since it was first reported in 1967. To increase our knowledge on the effects of genes in KD, we extracted statistically significant genes so far associated with this mysterious illness from candidate gene studies and genome-wide association studies. These genes contributed to susceptibility to KD, coronary artery lesions, resistance to initial IVIG treatment, incomplete KD, and so on. Gene ontology category and pathways were analyzed for relationships among these statistically significant genes. These genes were represented in a variety of functional categories, including immune response, inflammatory response, and cellular calcium ion homeostasis. They were mainly enriched in the pathway of immune response. We further highlighted the compelling immune pathway of NF-AT signal and leukocyte interactions combined with another transcription factor NF- κ B in the pathogenesis of KD. STRING analysis, a network analysis focusing on protein interactions, validated close contact between these genes and implied the importance of this pathway. This data will contribute to understanding pathogenesis of KD.


Assuntos
Redes Reguladoras de Genes , Predisposição Genética para Doença , Redes e Vias Metabólicas , Síndrome de Linfonodos Mucocutâneos/fisiopatologia , Criança , Biologia Computacional/métodos , Bases de Dados Genéticas , Estudo de Associação Genômica Ampla , Humanos , Inflamação , Leucócitos/citologia , Desequilíbrio de Ligação , Modelos Estatísticos , NF-kappa B/metabolismo , Fatores de Transcrição NFATC/metabolismo , Polimorfismo de Nucleotídeo Único , Transdução de Sinais
17.
Guang Pu Xue Yu Guang Pu Fen Xi ; 31(2): 452-5, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21510402

RESUMO

Based on laser induced breakdown spectroscopy data analysis, a quantitative determination method of elements was introduced. This method takes the advantage of Fourier analysis to study different part of the spectrum-white noise, thermal radiation and emission spectral lines. Through band-pass filter, emission spectral lines and most noise were separated, only a little white noise was missed. By calculated similarity between detected line and its corresponding unit characteristic line, convolution intensity was introduced to measure the detected line intensity. With Cu 324.75 nm as the analysis line and Ti 337.28 nm as the base line, the way of separating spectrum mentioned before was carried out to detect trace Cu pollution in soil. Results contained two bright spots. First, The correlation coefficient between Cu concentration and the spectral intensity was up to 0.997 9. Last but not the least, the detectability of Cu in soil was 44 mg x kg(-1), which is below the trace element thresholds for Class 2 soil used in the environmental quality standard for soil in China. At the same time, the relative error is always below 10%. Therefore the feasibility of this method for portable and instant detection of heavy metals in polluted soil was verified.

18.
Chem Asian J ; 6(1): 220-5, 2011 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-21077095

RESUMO

The first catalytic asymmetric conjugate addition of 1,3-dicarbonyl compounds to nitroenynes catalyzed by cinchona alkaloid-based thiourea organocatalysts has been developed. The 1,4-addition adducts were obtained solely, in moderate to good yields (up to 93%) with good enantioselectivities (up to 99% ee). This protocol affords a conceptually different entry to the precursors of pharmaceutically important chiral ß-alkynyl acid derivatives and synthetically useful chiral nitroalkynes. Notably, the protocol worked well with both aryl- and alkyl-substituted alkynyl substrates.


Assuntos
Alcenos/química , Malonatos/química , Tioureia/química , Catálise , Alcaloides de Cinchona/química , Estrutura Molecular , Estereoisomerismo
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