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1.
Sci Total Environ ; 858(Pt 3): 160089, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36370800

RESUMO

TBBPA bis(2-hydroxyethyl) ether (TBBPA-DHEE) pollution in the environment has raised serious public health concerns due to its potential neuroendocrine-disrupting effects. The neuroendocrine-disrupting effects of TBBPA-DHEE on marine spices, on the other hand, have received little attention. The behavioral, neuroendocrine-disrupting, and possible reproductive toxicity of TBBPA-DHEE were assessed in sexual developing zebrafish treated for 40 days by examining locomotor activity, Gonadotrophin releasing hormone (GnRH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels, and quantifying gene expression. In addition, transcriptome profiling was carried out to explore the possible mechanisms. According to our findings, TBBPA-DHEE treated zebrafish showed altered locomotor activity, a potential neuroendocrine-disrupting effect via the toxic effect on the hypothalamus and pituitary gland, which is evident in decreased levels of GnRH, FSH, and LH, according to our findings. The transcriptomic profiling reveals that a total of 216 DEGs were detected (5 upregulated and 211 down-regulated). Transcriptomic analysis shows that TBBPA-DHEE exposure caused decreased transcript levels of genes (cyp11a1, ccna1, ccnb2, ccnb1, cpeb1b, wee2) involved in cell cycle oocyte meiosis, progesterone mediated oocyte maturation, and ovarian steroidogenesis, which are known reproduction-related pathways. Overall, these findings add to our understanding of the impact of TBBPA-DHEE and biomonitoring in the maritime environment.

2.
Cardiol Res Pract ; 2022: 2370802, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36407284

RESUMO

Background: Myocardial bridging (MB) is a congenital anomaly involving the myocardial tissue encasement of a segment of the coronary artery. The purpose of the present study was to assess safety and efficacy of two surgical methods used for treating MB patients at our institute. Methods: Off-pump MB unroofing was performed in 45 adult patients between January 2016 and December 2021 by traditional surgical unroofing techniques (conventional group, n = 26) and blunt dissection techniques (blunt dissection group, n = 19). We retrospectively reviewed our patients by examining the baseline clinical characteristics, risk factors, medications, and diagnostic data for coronary artery disease. The Seattle Angina Questionnaire (SAQ) was used to assess angina symptoms both preoperatively and 6 months postsurgery. Results: No significant difference in preoperative clinical characteristics was observed between the two groups. The blunt dissection group had shorter unroofed period (14.69 vs. 18.91 mins, P=0.001), less ventilator time (16.26 vs. 24.62 hours, P < 0.001), and a shorter hospital stay (8.74 vs. 12.89 days, P < 0.001). Although both traditional and blunt dissection techniques significantly improved postoperative SAQ scores including physical limitation due to angina, anginal stability, anginal frequency, treatment satisfaction, and quality of life (P < 0.001), no significant difference was observed between the traditional and blunt dissection techniques for SAQ. No cases of left anterior descending (LAD) injury in the blunt dissection group were observed although seven patients in the conventional group had LAD injuries. Conclusions: In our single-center experience of MB unroofing, the blunt dissection technique is a safe, effective technique that significantly reduces surgical and ventilator time and hospital stay. MB patients with severe angina who underwent the blunt dissection for surgical unroofing experienced significant improvements in anginal symptoms and quality of life six months after the surgery.

3.
Perfusion ; : 2676591221131217, 2022 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-36206156

RESUMO

INTRODUCTION: Although venovenous extracorporeal membrane oxygenation (VV ECMO) is a reasonable salvage treatment for acute respiratory distress syndrome (ARDS), it requires sedating the patient. Sevoflurane and propofol have pulmonary protective and immunomodulatory properties. This study aimed to compare the effectiveness of sevoflurane and propofol on rats with induced ARDS undergoing VV ECMO. METHODS: Fifteen sprague-dawley (SD) rats were randomly divided into three groups: Con group, sevoflurane (Sevo) group and propofol (Pro) group. Arterial blood gas tests were performed at time pointsT0 (baseline), T1 (the time to ARDS), and T2 (weaning from ECMO). Oxygenation index (PaO2/FiO2) was calculated, and lung edema assessed by determining the lung wet:dry ratio. The protein concentration in bronchial alveolar lavage fluid (BALF) was determined by using bicinchoninic acid assay. Haematoxylin and eosin staining was used to evaluate the lung pathological scores in each group. IL-1ß and TNF-α were also measured in the BALF, serum and lung. RESULTS: Oxygenation index showed improvement in the Sevo group versus Pro group. The wet:dry ratio was reduced in the Sevo group compared with propofol-treated rats. Lung pathological scores were substantially lower in the Sevo group versus the Pro group. Protein concentrations in the BALF and levels of IL-1ß and TNF-α in the Sevo group were substantially lower versus Pro group. CONCLUSION: This study demonstrates that compared with propofol, sevoflurane was more efficacious in improving oxygenation and decreasing inflammatory response in rat models with ARDS subject to VV ECMO treatment.

4.
Front Immunol ; 13: 879775, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36090984

RESUMO

Arabinogalactan (AG) participates in forming the cell wall core of mycobacteria, a structure known as the mAGP complex. Few studies have reported the virulence of inartificial AG or its interaction with the host immune system. Using clustered regularly interspaced short palindromic repeats interference gene editing technology, conditional Mycobacterium marinum mutants were constructed with a low expression of embA or glfT2 (EmbA_KD or GlfT2_KD), which are separately involved in the biosynthesis of AG arabinose and galactose domains. High-performance gel permeation chromatography and high-performance liquid chromatography assays confirmed that the EmbA_KD strain showed a remarkable decrease in AG content with fragmentary arabinose chains, and the GlfT2_KD strain displayed less reduction in content with cut-down galactose chains. Based on transmission and scanning electron microscopy observations, the cell walls of the two mutants were found to be dramatically thickened, and the boundaries of different layers were more distinct. Phenotypes including the over-secretion of extracellular substances and enhanced spreading motility with a concomitant decreased resistance to ethambutol appeared in the EmbA_KD strain. The EmbA_KD and GlfT2_KD strains displayed limited intracellular proliferation after infecting murine J774A.1 macrophages. The disease progression infected with the EmbA_KD or GlfT2_KD strain significantly slowed down in zebrafish/murine tail infection models as well. Through transcriptome profiling, macrophages infected by EmbA_KD/GlfT2_KD strains showed enhanced oxidative metabolism. The cell survival measured using the CCK8 assay of macrophages exposed to the EmbA_KD strain was upregulated and consistent with the pathway enrichment analysis of differentially expressed genes in terms of cell cycle/apoptosis. The overexpression of C/EBPß and the increasing secretion of proinflammatory cytokines were validated in the macrophages infected by the EmbA_KD mutant. In conclusion, the AG of Mycobacterium appears to restrain the host innate immune responses to enhance intracellular proliferation by interfering with oxidative metabolism and causing macrophage death. The arabinose chains of AG influence the Mycobacterium virulence and pathogenicity to a greater extent.


Assuntos
Mycobacterium marinum , Animais , Arabinose , Galactanos , Galactose , Imunidade Inata , Camundongos , Virulência , Peixe-Zebra
5.
J Environ Manage ; 322: 116123, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36063698

RESUMO

Marine oil spills emanating from wells, pipelines, freighters, tankers, and storage facilities draw public attention and necessitate quick and environmentally friendly response measures. It is sometimes feasible to contain the oil with booms and collect it with skimmers or burn it, but this is impracticable in many circumstances, and all that can be done without causing further environmental damage is adopting natural attenuation, particularly through microbial biodegradation. Biodegradation can be aided by carefully supplying biologically accessible nitrogen and phosphorus to alleviate some of the microbial growth constraints at the shoreline. This review discussed the characteristics of oil spills, origin, ecotoxicology, health impact of marine oils spills, and responses, including the variety of remedies and responses to oil spills using biological techniques. The different bioremediation and bio-dispersant treatment technologies are then described, with a focus on the use of green surfactants and their advances, benefits/drawbacks. These technologies were thoroughly explained, with a timeline of research and recent studies. Finally, the hurdles that persist as a result of spills are explored, as well as the measures that must be taken and the potential for the development of existing treatment technologies, all of which must be linked to the application of integrated procedures.


Assuntos
Poluição por Petróleo , Petróleo , Poluentes Químicos da Água , Biodegradação Ambiental , Nitrogênio , Óleos , Petróleo/metabolismo , Poluição por Petróleo/análise , Fósforo , Água do Mar , Tensoativos , Poluentes Químicos da Água/análise
6.
Artigo em Inglês | MEDLINE | ID: mdl-36113845

RESUMO

Tetrabromobisphenol A bis (2-hydroxyethyl ether) (TBBPA-DHEE) is a derivative of Tetrabromobisphenol A (TBBPA) used as an intermediate flame retardant in engineering polymers. The mechanism of neurodevelopmental toxicity of TBBPA-DHEE remains unclear due to limited toxicological data. We performed behavioral and transcriptomic analyses to assess the neurodevelopmental effects of TBBPA-DHEE on developing zebrafish and potential toxicity mechanisms. Our result shows that exposure to TBBPA-DHEE significantly increased mortality, deformity rate, and reduction in hatch rate, hatchability, and body length relative to the DMSO control. The behavior analysis indicates that TBBPA-DHEE significantly reduced the spontaneous movement of larva compared to the control. The TSH and GH levels were significantly reduced in all the exposure groups in a concentration-dependent manner relative to the DMSO control. TBBPA-DHEE exhibited a significant reduction in locomotor activity across all the exposure groups in the light/dark locomotion test. The transcriptomic analysis result shows that 579 genes were differentially expressed. KEGG analysis shows the enrichment of complement cascade, JAK-STAT signaling pathway, cytokine-cytokine interaction, and phototransduction pathway resulting in a change in mRNA expression of their genes. These observed changes in developmental endpoints, hormonal level, and alteration in mRNA expression of component genes involved in neurodevelopmental pathways could be part of the possible mechanism of the observed toxic effects of TBBPA-DHEE exposure on zebrafish. This study could reveal the possible neurodevelopmental toxicity of TBBPA-DHEE to aquatic species, which could help uncover the health implications of emerging environmental contaminants.


Assuntos
Retardadores de Chama , Bifenil Polibromatos , Poluentes Químicos da Água , Animais , Citocinas/metabolismo , Dimetil Sulfóxido/metabolismo , Éter/metabolismo , Éteres/análise , Éteres/metabolismo , Retardadores de Chama/toxicidade , Bifenil Polibromatos/análise , Bifenil Polibromatos/metabolismo , Bifenil Polibromatos/toxicidade , Polímeros , RNA Mensageiro/metabolismo , Tireotropina/genética , Tireotropina/metabolismo , Transcriptoma , Poluentes Químicos da Água/metabolismo , Peixe-Zebra/genética , Peixe-Zebra/metabolismo
7.
Chemosphere ; 307(Pt 3): 136032, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35977571

RESUMO

Accumulation and concomitant risk of metals in plastic-shed soil (PSS)-vegetable system around industrial areas have attracted growing public concern recently, while limited studies have focused on human bioaccessible metals in various plastic-shed vegetables and health risk calculated using bioaccessible metals. Previous studies showed that intensive farming and industrial activities could prominently affect metal migration from PSS to vegetables via altering PSS pH, total and bioavailable metal contents. In contrast, whether changes in PSS pH and metal contents control bioaccessible metals in vegetables and health risk is still unknown. For PSS management and sustainable plastic-shed vegetable production in the areas with rapid industrialization, 41 PSS and 32 plastic-shed vegetable samples were sampled from the industrial areas of Yangtze River Delta, China to systematically clarify the specific connections among anthropogenic activities, soil pH and metal contents, and metal transfer and health risk in PSS-vegetable-human system. The results indicated that Cr and Cd contents in 15.6% and 9.38% of vegetable samples exceeded the allowable limits in China. Tolerable cancer risk existed and was mainly induced by bioaccessible Cr in vegetables. Decreased PSS pH mainly caused by heavy use of nitrogen fertilizers increased bioavailable Ni, Cd, Zn, Pb, and Cu in PSS and subsequently enhanced their total and bioaccessible contents in vegetables. Prominent Cr accumulation in PSS induced by industrial wastewater irrigation exacerbated Cr uptake by vegetables, which increased bioaccessible Cr in vegetables and contributed greatly to cancer risk. To reduce transfer and health risk especially of Cd and Cr in the food chain, some appropriate measures related to source control and remediation should be proposed for preventing and mitigating PSS acidification and Cr accumulation.


Assuntos
Metais Pesados , Neoplasias , Poluentes do Solo , Efeitos Antropogênicos , Cádmio , China , Monitoramento Ambiental , Fertilizantes/análise , Humanos , Concentração de Íons de Hidrogênio , Chumbo , Metais Pesados/análise , Nitrogênio , Plásticos , Medição de Risco , Solo/química , Poluentes do Solo/análise , Verduras/química , Águas Residuárias
8.
Environ Sci Pollut Res Int ; 29(47): 70611-70634, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35994149

RESUMO

Microplastics (MPs), over the years, have been regarded as a severe environmental nuisance with adverse effects on our ecosystem as well as human health globally. In recent times, microplastics have been reported to support biofouling by genetically diverse organisms resulting in the formation of biofilms. Biofilms, however, could result in changes in the physicochemical properties of microplastics, such as their buoyancy and roughness. Many scholars perceived the microplastic-biofilm association as having more severe consequences, providing evidence of its effects on the environment, aquatic life, and nutrient cycles. Furthermore, other researchers have shown that microplastic-associated biofilms have severe consequences on human health as they serve as vectors of heavy metals, toxic chemicals, and antibiotic resistance genes. Despite what is already known about their adverse effects, other interesting avenues are yet to be fully explored or developed to turn the perceived negative microplastic-biofilm association to our advantage. The major inclusion criteria for relevant literature were that it must focus on microplastic association biofilms, while we excluded papers solely on biofilms or microplastics. A total of 242 scientific records were obtained. More than 90% focused on explaining the environmental and health impacts of microplastic-biofilm association, whereas only very few studies have reported the possibilities and opportunities in turning the microplastic biofilms association into benefits. In summary, this paper concisely reviews the current knowledge of microplastic-associated biofilms and their adverse consequences and further proposes some approaches that can be developed to turn the negative association into positive.


Assuntos
Microplásticos , Poluentes Químicos da Água , Biofilmes , Ecossistema , Monitoramento Ambiental , Humanos , Plásticos , Poluentes Químicos da Água/análise
9.
J Heart Lung Transplant ; 41(10): 1391-1400, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-35973885

RESUMO

BACKGROUND: The preferred configuration for bridging patients with respiratory failure while awaiting lung transplantation is venovenous extracorporeal membrane oxygenation (VV ECMO). However, the protective effect of VV ECMO on the lung, as well as the underlying mechanisms, are still unknown. METHODS: We investigated the role of VV ECMO in preventing lung injury in vivo using a rat model. Additionally, the effects of Hippo/YAP signaling on alveolar epithelial type II cells (AT2)-mediated alveolar epithelial recovery in VV ECMO rats were also investigated. In the bronchoalveolar lavage fluid (BALF) and lung tissue, RNA sequencing, lung injury, edema, and cytokine expression were evaluated. RESULTS: VV ECMO significantly improved severe hypoxemia, reduced lung edema, and inflammatory response, and altered alveolar epithelial function, as indicated by reduced protein concentrations in BALF. This was associated with Hippo/YAP signaling activation, according to RNA sequencing analysis. Furthermore, we discovered that after VV ECMO, AT2 cells proliferated and differentiated, and this increase in AT2 cell activity was correlated to the increased nuclear expression of YAP, which is critical for alveolar epithelial recovery from lung injury. During VV ECMO, verteporfin-induced YAP inhibition and the loss of the oxygenator delayed lung alveolar epithelial recovery and led to a prolonged inflammatory response. CONCLUSIONS: These findings suggest that VV ECMO protects against lung injury by activating the Hippo/YAP signaling pathway. Strategies aimed at increasing YAP activity in AT2 cells could thus aid alveolar epithelial recovery, making VV ECMO easier for lung transplantation.


Assuntos
Oxigenação por Membrana Extracorpórea , Lesão Pulmonar , Animais , Citocinas , Edema , Lesão Pulmonar/terapia , Ratos , Verteporfina
10.
Artigo em Inglês | MEDLINE | ID: mdl-35872240

RESUMO

Amitriptyline (AMI), the most commonly prescribed tricyclic antidepressant, is widely detected in water environments. Exposure to AMI may lead to diverse adverse effects on aquatic organisms, but little is known about the effect of short-term exposure to AMI on the gut microbiota of aquatic organisms and their recovery characteristics. In the present study, adult zebrafish (Danio rerio) were exposed to AMI (0, 2.5, 10, and 40 µg/L) for seven days, and then allowed to recover in AMI-free culture water for 21 days. The exposure caused gut damages in all the AMI treated groups of zebrafish, which became more severe after recovery compared to the control group. AMI exposure also disturbed the microbiota of zebrafish guts and rearing water even after the 21-day recovery period. Furthermore, AMI exposure affected microbes involved in the substance and energy metabolic functions in zebrafish guts and tended to increase the abundance of microbial genera associated with opportunistic pathogens. In addition, the microbial predicted metabolic functions in AMI-exposed guts of zebrafish were significantly altered after the 21-day recovery period, explaining the persistent effects of short-term exposure to AMI. The results of this study suggest that acute exposure to AMI may have persistent impacts on the gut histomorphology and the gut microbiota in aquatic organisms.


Assuntos
Microbioma Gastrointestinal , Poluentes Químicos da Água , Amitriptilina/toxicidade , Animais , Disbiose/induzido quimicamente , Água , Poluentes Químicos da Água/toxicidade , Peixe-Zebra
11.
Artigo em Inglês | MEDLINE | ID: mdl-35896870

RESUMO

Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by insulin resistance (IR) and has attracted worldwide attention due to its high prevalence. As a typical persistent organic pollutant, decabromodiphenyl ether (BDE-209) has been detected in food and human samples, and the concentration trends increase year by year. In addition, it has been proved to have the potential to increase the risk of IR, but it is rarely reported whether it could aggravate IR in T2DM. Therefore, in this study, the IR-BRL (buffalo rat liver cells with IR) model was applied to study the metabolism toxicity and susceptibility of BDE-209. Results showed that BDE-209 could inhibit glucose absorption and increase the levels of serum total cholesterol (TC) and triglyceride (TG), ultimately leading to the disorder of glucolipid metabolism in IR-BRL cells. Besides, it also could cause cell damage by increasing the levels of aspartate transaminase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA) in cells. Moreover, its potential mechanisms were to: (1) affect the transport of glucose, synthesis of glycogen and fatty acid via IRS-1/GLUT4 and IRS-1/PI3K/AKT/GSK-3ß pathways; (2) impact the proliferation and differentiation by regulating the expression of Mek1/2, Erk1/2, and mTOR proteins and genes. Furthermore, susceptibility analysis showed that there was a significant synergism interaction between IR and BDE-209, which suggested that IR-BRL cells were more susceptible to the metabolism toxicity induced by BDE-209.

12.
Artigo em Inglês | MEDLINE | ID: mdl-35700941

RESUMO

Cetylpyridinium chloride (CPC) is a cationic surfactant that has been widely used as an antibacterial ingredient in pharmaceutical and personal care products. Due to its high residue in surface waters, there is increasing concern over the potential risk of CPC to aquatic ecosystems. However, knowledge of its impacts on fish is still limited. Therefore, this study exposed juvenile and adult zebrafish to CPC (0, 10, and 40 µg/L) for four days. Subsequently, changes in their behavioral traits and brain levels of several neurotransmitters were investigated. The behavioral assay showed that CPC exposure significantly decreased the locomotor activity and social interaction of zebrafish at both life stages, and juveniles were more sensitive to CPC exposure than adults. In the control groups, the brain neurotransmitters concentrations increased with age in zebrafish. However, CPC exposure tended to increase the brain neurotransmitter levels of juveniles but decreased their levels in adults. Correlation analysis revealed that the brain monoamine neurotransmitters and their turnover might play important roles in the life stage-dependent behavioral response to CPC. In particular, the DOPAC/DA ratio was significantly associated with CPC-induced hypoactivity and reduced social interactions in juveniles but not adults. Our findings demonstrated that CPC exposure could cause abnormal behavior in juvenile and adult zebrafish and disturb their brain neurotransmitters, even at environmentally relevant concentrations, and thus highlighted the necessity for further assessing its potential risks to aquatic ecosystems.


Assuntos
Cetilpiridínio , Poluentes Químicos da Água , Animais , Encéfalo , Cetilpiridínio/farmacologia , Ecossistema , Neurotransmissores , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/fisiologia
13.
Phys Chem Chem Phys ; 24(19): 11455-11470, 2022 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-35514325

RESUMO

Lanthanide-based nanocrystals possess three unique physical properties that make them attractive for facilitating photoreactions, namely photon upconversion luminescence, Lewis acid catalytic activity and photothermal properties. When co-doped with a suitable sensitizer and activator lanthanide ions, rare-earth fluoride nanocrystals upconvert near-infrared light to higher energy photons that can be used to excite photosensitizers that absorb ultraviolet and visible light in photocatalytic and photopolymerization reactions. Surface lanthanide ions on nanocrystals also have the propensity to behave as Lewis acid (LA) catalytic sites. In addition, NIR-light excited lanthanides such as Nd3+ undergo cross-relaxation interaction with neighbouring ground-state ions followed by non-radiative decay to generate heat (i.e., photothermal) which enhances the rate of chemical reactions. In this perspective, we provide a survey of the recent progress in the use of lanthanide-based nanocrystals as upconverting nanolamps, LA catalysts and photothermal nanoheaters in driving synthetic and polymerization reactions, and the challenges that need to be further addressed in order for this vibrant research area to develop and grow.

14.
J Colloid Interface Sci ; 623: 109-123, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35569220

RESUMO

In this work, a novel hierarchical 1D/2D core/shell Sb2S3-ZnIn2S4 (SB-ZIS) heterostructure with highly efficient photocatalytic activities for both hydrogen production from water and organic pollutant degradation was designed and fabricated via a simple one-step hydrothermal method. The as-prepared SB-ZIS heterostructure, where ZnIn2S4 nanosheets uniformly grew onto Sb2S3 nanorod to form a tight and large intimate contacted interface, was conducive to improve the absorption capacity of light, increase the surface area, shorten the distance of electronic transmission channels and accelerate the separation and migration of photogenerated carriers. As a result, the presented SB-ZIS composites demonstrated significantly enhanced photocatalytic performances for H2 generation and Tetracycline Hydrochloride (TCH) photodegradation. The photocatalytic H2 production rate of optimal SB-ZIS-2 sample (1685.14 µmol·g-1·h-1) was about 12.24 times as large as that of pure ZnIn2S4 (137.63 µmol·g-1·h-1). The apparent quantum efficiency (AQE) at 420 nm was up to 3.8%. In addition, the highest rate constant for TCH removal (0.514 h-1) was 20.3 and 2.89 times larger than those of pure Sb2S3 and Znln2S4, respectively. The possible reaction routes of TCH and the photocatalytic reaction mechanism of SB-ZIS sample were also discussed in detail. This work will provide some useful information for the development of dual-functional Sb2S3-based type I core-shell heterostructure with an efficient photocatalytic activity for solving environmental pollution and producing clean hydrogen energy.


Assuntos
Poluentes Ambientais , Nanotubos , Hidrogênio/química , Fotólise , Tetraciclina/química
15.
Perfusion ; : 2676591221104302, 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35608047

RESUMO

OBJECTIVE: Although the application of venovenous extracorporeal membrane oxygenation (VV-ECMO) in coronavirus disease 2019 (COVID-19) patients with acute respiratory distress syndrome (ARDS) is accumulating, the feasibility and safety of this therapy remain controversial. We aimed to evaluate the effect of VV-ECMO in the treatment of these patients. METHODS: A comprehensive literature search was performed using PubMed, Embase, the Cochrane Library, and International Clinical Trials Registry Platform databases through November 2021. According to the inclusion and exclusion criteria, the included studies were screened, and meta-analysis was performed by R software (version 4.0.2). RESULTS: Forty-two studies including 2037 COVID-19 patients supported with VV-ECMO due to ARDS were identified. The pooled analysis revealed that 30-, 60-, and 90-day mortality among patients were respectively 46% (95% CI 37%-57%, I2 = 66%), 46% (95% CI 30%-70%, I2 = 93%), and 49% (95% CI 43%-58%, I2 = 52%), and the pooled incidence rate of in-hospital mortality, major bleeding, hemorrhagic stroke, thrombosis, pulmonary embolism, deep venous thrombosis, and renal replacement therapy were respectively 35%, 39%, 11%, 40%, 15%, 21%, and 44%. CONCLUSION: Although COVID-19 patients may have a higher risk of bleeding, hemorrhagic stroke, and acute kidney injury during ECMO therapy, the survival rate was more than half of the cases. Our data may support the application of VV-ECMO in COVID-19 patients.

16.
Artigo em Inglês | MEDLINE | ID: mdl-35640788

RESUMO

Tetrabromobisphenol A bis(2-hydroxyetyl) ether (TBBPA-DHEE) is among the main derivatives of Tetrabromobisphenol A (TBBPA). Result from previous study showed that TBBPA-DHEE can cause neurotoxicity in rat. In this study, zebrafish larvae were used for evaluation of TBBPA-DHEE-induced developmental toxicity, apoptosis, oxidative stress and the potential molecular mechanisms of action. Our result showed that TBBPA-DHEE exposure caused a significant concentration-dependent developmental toxicity endpoints like death rate, malformation rate, growth rate. TBBPA-DHEE altered locomotor and enzymes activities of larvae and caused apoptosis within the brain indicating the potential TBBPA-DHEE-induced cardiac, brain impairment in the zebrafish larvae. Our transcriptomic analysis shows that 691 genes were differentially expressed (DEGs) (539 upregulated, 152 downregulated). The KEGG and GO enrichment pathway analysis shows that the DEGs were involved in development, immunity, enzyme activity. Our study provides novel evidence on the neurodevelopmental toxicity and toxicity mechanism of TBBPA-DHEE which are vital for assessment of the environmental toxicity and risk assessment of the chemical.


Assuntos
Retardadores de Chama , Bifenil Polibromatos , Animais , Éter/metabolismo , Éteres/análise , Éteres/metabolismo , Retardadores de Chama/toxicidade , Larva/metabolismo , Bifenil Polibromatos/análise , Bifenil Polibromatos/química , Bifenil Polibromatos/toxicidade , Ratos , Transcriptoma , Peixe-Zebra/metabolismo
17.
Antioxidants (Basel) ; 11(5)2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35624808

RESUMO

The combined application of nanoparticles and surfactants has attracted tremendous attention in basic research and industry. However, knowledge of their combined toxicity remains scarce. In this study, we exposed zebrafish embryos to cetylpyridinium chloride (CPC, a cationic surfactant, at 0 and 20 µg/L), zeolitic imidazolate framework nanoparticles (ZIF-NPs, at 0, 30, and 60 mg/L), and their mixtures until 120 h post-fertilization (hpf). Within the used concentration range, both single and combined exposures exhibited limited effects on the survival and hatching of zebrafish. However, the combined exposure of ZIF-NPs and CPC caused more severe effects on the heart rate at both 48 and 72 hpf. The combined exposure also induced significant hyperactivity (i.e., increasing the average swimming velocity) and oxidative stress in zebrafish larvae (at 120 hpf), although all single exposure treatments exhibited limited impacts. Furthermore, the level of reactive oxygen species (or malondialdehyde) exhibited a significantly positive correlation with the heart rate (or average swimming velocity) of zebrafish, suggesting that oxidative stress plays a role in mediating the combined toxicity of CPC and ZIF-NPs to zebrafish. Our findings suggest that the interaction of CPC and ZIF-NPs should not be ignored when assessing the potential risks of their mixtures.

18.
Antioxidants (Basel) ; 11(4)2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35453362

RESUMO

Cetylpyridinium chloride (CPC) is a widely used surfactant that has been detected in various water ecosystems. However, knowledge on the toxicity of CPC to fish remains scarce. Here, we examined the survival, development, behavior, and oxidative stress in the early life stages of zebrafish exposed to CPC (0, 4, 40, 400, and 1200 µg/L) until 120 h post-fertilization (hpf). Results showed that CPC induced significant mortality at 400 and 1200 µg/L, with a 120 h-EC50 value of 175.9 µg/L. CPC significantly decreased the heart rate of embryos (48 hpf; 4-400 µg/L) and larvae (72 hpf; 40 and 400 µg/L). At 120 hpf, CPC exhibited a dual effect on the locomotion activity (decreased at 400 µg/L and increased at 4 and 40 µg/L) and elevated the reactive oxygen species, superoxide dismutase, and glutathione levels in zebrafish larvae at 400 µg/L. In addition, a correlation analysis revealed that CPC-induced oxidative stress might play a critical role in mediating the cardiac and behavioral toxicity of CPC to zebrafish larvae. Our findings suggest that CPC may disturb the fish's development, behavior, and oxidative status at environmentally relevant concentrations, which should not be ignored when assessing its potential risks to aquatic ecosystems.

19.
EMBO Rep ; 23(6): e53932, 2022 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-35403787

RESUMO

Aberrant activation of stimulator of interferon genes (STING) is tightly associated with multiple types of disease, including cancer, infection, and autoimmune diseases. However, the development of STING modulators for the therapy of STING-related diseases is still an unmet clinical need. We employed a high-throughput screening approach based on the interaction of small-molecule chemical compounds with recombinant STING protein to identify functional STING modulators. Intriguingly, the cyclin-dependent protein kinase (CDK) inhibitor Palbociclib was found to directly bind STING and inhibit its activation in both mouse and human cells. Mechanistically, Palbociclib targets Y167 of STING to block its dimerization, its binding with cyclic dinucleotides, and its trafficking. Importantly, Palbociclib alleviates autoimmune disease features induced by dextran sulphate sodium or genetic ablation of three prime repair exonuclease 1 (Trex1) in mice in a STING-dependent manner. Our work identifies Palbociclib as a novel pharmacological inhibitor of STING that abrogates its homodimerization and provides a basis for the fast repurposing of this Food and Drug Administration-approved drug for the therapy of autoinflammatory diseases.


Assuntos
Doenças Autoimunes , Neoplasias , Animais , Doenças Autoimunes/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos , Neoplasias/metabolismo , Piperazinas/farmacologia , Piridinas/farmacologia , Piridinas/uso terapêutico
20.
Mol Cell ; 82(11): 2032-2049.e7, 2022 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-35460603

RESUMO

Virus infection modulates both host immunity and host genomic stability. Poly(ADP-ribose) polymerase 1 (PARP1) is a key nuclear sensor of DNA damage, which maintains genomic integrity, and the successful application of PARP1 inhibitors for clinical anti-cancer therapy has lasted for decades. However, precisely how PARP1 gains access to cytoplasm and regulates antiviral immunity remains unknown. Here, we report that DNA virus induces a reactive nitrogen species (RNS)-dependent DNA damage and activates DNA-dependent protein kinase (DNA-PK). Activated DNA-PK phosphorylates PARP1 on Thr594, thus facilitating the cytoplasmic translocation of PARP1 to inhibit the antiviral immunity both in vitro and in vivo. Mechanistically, cytoplasmic PARP1 interacts with and directly PARylates cyclic GMP-AMP synthase (cGAS) on Asp191 to inhibit its DNA-binding ability. Together, our findings uncover an essential role of PARP1 in linking virus-induced genome instability with inhibition of host immunity, which is of relevance to cancer, autoinflammation, and other diseases.


Assuntos
Antivirais , Nucleotidiltransferases , Antivirais/farmacologia , Citoplasma/genética , Citoplasma/metabolismo , DNA , Dano ao DNA , Instabilidade Genômica , Humanos , Nucleotidiltransferases/genética , Nucleotidiltransferases/metabolismo , Poli(ADP-Ribose) Polimerase-1/metabolismo
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