Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 223
Filtrar
1.
Food Chem ; 372: 131146, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34627091

RESUMO

Because of the nutritional advantages and customer acceptance, it is vital to ensure pork meat quality. This study examined the quantification of myoglobin proportions (deoxymyoglobin and oxymyoglobin) by coupling Raman spectroscopy with efficient variables selection chemometrics. Prior to acquiring Raman spectroscopic data, the fractions of myoglobin were determined. Afterward, multivariate calibration methods like partial least square (PLS), competitive adaptive reweighted sampling (CARS-PLS), genetic algorithm-PLS (GA-PLS), and random frog-PLS (RF-PLS) were applied and evaluated. The models' performance was assessed using correlation coefficients of prediction (Rp), root mean square error of prediction (RMSEP), and residual predictive deviation (RPD). The RF-PLS model achieved optimal results for both deoxymyoglobin and oxymyoglobin, with Rp = 0.8936; RMSEP = 2.91 and RPD = 1.97 for the former and Rp = 0.9762; RMSEP = 1.23 and RPD = 4.47 for the latter, respectively. Therefore, this work demonstrated that Raman spectroscopy paired with RF-PLS could be employed for nondestructive, fast, and easy detection of deoxymyoglobin and oxymyoglobin.

3.
Perfusion ; : 2676591211031095, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34263684

RESUMO

OBJECTIVE: Although the application of del Nido cardioplegia solution (DNC) in adult cardiac surgery is accumulating, the feasibility and safety of this myocardial protection strategy in adults remains controversial. We aimed to update our previous meta-analysis to determine the myocardial protective effect of DNC versus conventional cardioplegia (CC) in adult cardiac surgery. METHODS: A comprehensive literature search was performed using PubMed, EMBASE, the Cochrane Library, and International Clinical Trials Registry Platform databases through November 2020. RESULTS: Thirty-seven observational studies and four randomized controlled trials (RCTs) including 21,779 patients were identified. The DNC group was associated with decreased postoperative cardiac enzymes [troponin T (cTnT) and creatine kinase-MB (CK-MB)] [standardized mean differences (SMD): -0.59, 95% confidence interval (CI): -0.99 to -0.19, p = 0.004], cardiopulmonary bypass (CPB) time (MD: -9.31, 95% CI: -13.10 to -5.51, p < 0.00001), aortic cross-clamp (ACC) time (MD: -7.20, 95% CI: -10.31 to -4.09, p < 0.00001), and cardioplegia volume (SMD: -1.95, 95% CI: -2.46 to -1.44, p < 0.00001). Intraoperative defibrillation requirement was less in the DNC group [relative risk (RR): 0.50, 95% CI: 0.33 to 0.75, p = 0.0007]. The pooled analysis revealed no significant difference in operative mortality among the patients assigned to DNC and those undergoing CC. CONCLUSION: In adult cardiac surgery, compared to CC, myocardial protection used with DNC yield similar or better short-term clinical outcomes. More high-quality trials and RCTs reflecting long-term follow-up morbidity and mortality are required in the future to confirm these findings.

4.
J Phys Chem B ; 125(30): 8550-8557, 2021 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-34286993

RESUMO

In the heterobiaryl cross-coupling reaction between aryl halides (Ar-X) and N-methylpyrrole (N-MP) catalyzed by rhodamine 6G (Rh6G+) under irradiation with visible light, a highly active and long-lived (millisecond time range) rhodamine 6G radical (Rh6G•) is formed upon electron transfer from N,N-diisopropylethylamine (DIPEA) to Rh6G+. In this study, we utilized steady-state and time-resolved spectroscopy techniques to demonstrate the existence of another electron-transfer process occurring from the relatively electron-rich N-MP to photoexcited Rh6G+ that was neglected in the previous reports. In this case, the radical Rh6G• formed is short-lived and undergoes rapid recombination (nanosecond time-range), rendering it ineffective in reducing Ar-X to aryl radicals Ar• that can subsequently be trapped by N-MP. This is further demonstrated via two model reactions involving 4'-bromoacetophenone and 1,3,5-tribromobenzene with insignificant product yields after visible-light irradiation in the absence of DIPEA. The unproductive quenching of photoexcited Rh6G+ by N-MP leads to a lower concentration of photocatalyst available for competitive charge transfer with DIPEA and hence decreases the efficiency of the cross-coupling reaction.


Assuntos
Elétrons , Pirróis , Rodaminas
5.
Radiother Oncol ; 161: 230-240, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34166717

RESUMO

BACKGROUND AND PURPOSE: To commission and implement an Autoencoder based Classification-Regression (ACLR) model for VMAT patient-specific quality assurance (PSQA) in a multi-institution scenario. MATERIALS AND METHODS: 1835 VMAT plans from seven institutions were collected for the ACLR model commissioning and multi-institutional validation. We established three scenarios to validate the gamma passing rates (GPRs) prediction and classification accuracy with the ACLR model for different delivery equipment, QA devices, and treatment planning systems (TPS). The prediction performance of the ACLR model was evaluated using mean absolute error (MAE) and root mean square error (RMSE). The classification performance was evaluated using sensitivity and specificity. An independent end-to-end test (E2E) and routine QA of the ACLR model were performed to validate the clinical use of the model. RESULTS: For multi-institution validations, the MAEs were 1.30-2.80% and 2.42-4.60% at 3%/3 mm and 3%/2 mm, respectively, and RMSEs were 1.55-2.98% and 2.83-4.95% at 3%/3 mm and 3%/2 mm, respectively, with different delivery equipment, QA devices, and TPS, while the sensitivity was 90% and specificity was 70.1% at 3%/2 mm. For the E2E, the deviations between the predicted and measured results were within 3%, and the model passed the consistency check for clinical implementation. The predicted results of the model were the same in daily QA, while the deviations between the repeated monthly measured GPRs were all within 2%. CONCLUSIONS: The performance of the ACLR model in multi-institution scenarios was validated on a large scale. Routine QA of the ACLR model was established and the model could be used for VMAT PSQA clinically.


Assuntos
Radioterapia de Intensidade Modulada , Raios gama , Humanos , Garantia da Qualidade dos Cuidados de Saúde , Radiometria , Dosagem Radioterapêutica , Planejamento da Radioterapia Assistida por Computador , Sensibilidade e Especificidade
6.
J Environ Sci (China) ; 105: 90-99, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34130843

RESUMO

The tetC gene has been found to be one of the most widely distributed tetracycline resistance (tet) genes in various environmental niches, but the detailed dissemination mechanisms are still largely unknown. In the present study, 11 tetC-containing Aeromonas media strains were isolated from an aerobic biofilm reactor under oxytetracycline stresses, and the genome of one strain was sequenced using the PacBio RSII sequencing approach to reveal the genetic environment of tetC. The tetC gene was carried by an IS26 composite transposon, named Tn6434. The tetC-carrying Tn6434 structure was detected in all of the A. media strains either in a novel plasmid pAeme2 (n=9) or other DNA molecules (n=2) by PCR screening. The NCBI database searching result shows that this structure was also present in the plasmids or chromosomes of other 13 genera, indicating the transferability of Tn6434. Inverse PCR and sequencing confirmed that Tn6434 can form a circular intermediate and is able to incorporate into a preexisting IS26 element, suggesting that Tn6434 might be responsible for the dissemination of tetC between different DNA molecules. This study will be helpful in uncovering the spread mechanism of tet genes in water environments.


Assuntos
Aeromonas , Oxitetraciclina , Aeromonas/genética , Antibacterianos , Biofilmes , Plasmídeos/genética
7.
Environ Sci Pollut Res Int ; 28(35): 49153-49165, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33932205

RESUMO

To explore the comprehensive utilization of agricultural wastes, solid-state fermentation was applied to residues of Flammulina velutipes (F. velutipes) and vinegar for use in culturing earthworms. Fermentation technology and earthworm culture technology were optimized by response surface methodology in this study. The optimal fermentation product for earthworm culture was obtained under an inoculum amount of 7.5%, fermentation temperature of 25.6 °C, pH 7.7 and protein content of 18.23%. The optimum culture conditions were a culture density of 18.4 individuals/dm3, an initial pH of 7.2 and a fermentation temperature of 26.8 °C. The daily weight gain multiplier of earthworms was 0.0387 units, and it increased significantly compared with that of the unfermented and cow dung groups. The fermented product of F. velutipes and vinegar residues could be used to culture earthworms and improve the metabolism and antioxidant capacities of earthworms. This provides a new way to comprehensively utilize agricultural waste resources.


Assuntos
Flammulina , Oligoquetos , Ácido Acético , Agricultura , Animais , Bovinos , Feminino , Fermentação , Humanos
8.
EMBO Rep ; 22(7): e51678, 2021 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-33987949

RESUMO

Mycobacterial arabinogalactan (AG) is an essential cell wall component of mycobacteria and a frequent structural and bio-synthetical target for anti-tuberculosis (TB) drug development. Here, we report that mycobacterial AG is recognized by galectin-9 and exacerbates mycobacterial infection. Administration of AG-specific aptamers inhibits cellular infiltration caused by Mycobacterium tuberculosis (Mtb) or Mycobacterium bovis BCG, and moderately increases survival of Mtb-infected mice or Mycobacterium marinum-infected zebrafish. AG interacts with carbohydrate recognition domain (CRD) 2 of galectin-9 with high affinity, and galectin-9 associates with transforming growth factor ß-activated kinase 1 (TAK1) via CRD2 to trigger subsequent activation of extracellular signal-regulated kinase (ERK) as well as induction of the expression of matrix metalloproteinases (MMPs). Moreover, deletion of galectin-9 or inhibition of MMPs blocks AG-induced pathological impairments in the lung, and the AG-galectin-9 axis aggravates the process of Mtb infection in mice. These results demonstrate that AG is an important virulence factor of mycobacteria and galectin-9 is a novel receptor for Mtb and other mycobacteria, paving the way for the development of novel effective TB immune modulators.


Assuntos
Mycobacterium tuberculosis , Peixe-Zebra , Animais , Galactanos , Galectinas/genética , Camundongos
9.
Artigo em Inglês | MEDLINE | ID: mdl-34043492

RESUMO

Rhodotorula mucilaginosa is an antagonistic yeast for which our research team has recently reported interesting biocontrol activities against blue mould decay of apples and a strong ability to decrease the patulin concentration in vivo. However, the possible mechanisms of patulin degradation by R. mucilaginosa and the toxicity of patulin degradation products remain unclear. In this study, the effect of R. mucilaginosa on patulin degradation and toxicity of degradation products were investigated, the results showed that viable cells of R. mucilaginosa are essential to patulin degradation. Also, R. mucilaginosa eliminated patulin without adsorbing it through its cell wall. The extracellular metabolites of R. mucilaginosa stimulated by patulin showed little degradation activity for patulin. Cycloheximide addition into the medium significantly decreased the patulin degradation capacity of R. mucilaginosa cells. The main patulin degradation product by R. mucilaginosa was ascladiol, which was proved non-toxic to human hepatoma (HepG2) cells at 0.625-10 g/mL. Furthermore, toxicological analysis using a confocal laser scanning microscope revealed that the degradation product induced cellular apoptosis to a lesser extent than patulin itself. This result offers an innovative method to detoxify patulin and limit the risks of patulin in fruits and vegetables using R. mucilaginosa.


Assuntos
Fungos/metabolismo , Furanos/toxicidade , Patulina/metabolismo , Rhodotorula/metabolismo , Cicloeximida/metabolismo , Aditivos Alimentares/metabolismo , Contaminação de Alimentos , Frutas/microbiologia , Fungos/crescimento & desenvolvimento , Células Hep G2 , Humanos , Malus/microbiologia , Metaboloma , Medição de Risco
10.
Food Chem ; 359: 129928, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33957331

RESUMO

Benchtop near-infrared (NIR) spectroscopy coupled with multivariate analysis was used for the classification and prediction of antioxidant properties of walnut. Total phenolic content (TPC), total flavonoid content (TFC), ABTS assay and FRAP assay were performed spectrophotometrically. The synergy interval partial least square coupled competitive adaptive reweighted sampling (Si-CARS-PLS) was used for the prediction. A decent discrimination using principal component analysis (PCA) was observed by mean of spectroscopic and antioxidant properties data with total cumulative variance of 99.26% (PC1 = 95.07%, PC2 = 2.98%, PC3 = 1.21%) and 96.60% (PC1 = 64.28%, PC2 = 32.32%) respectively. The Si-CARS-PLS yielded optimal performance, RP = 0.9616, RPD = 3.807 for TPC, RP = 0.9657, RPD = 3.367 for TFC, RP = 0.9683, RPD = 2.728 for ABTS assay, and RP = 0.914, RPD = 2.669 for FRAP assay. These findings revealed that NIR integrated with Si-CARS-PLS could be used for the prediction of antioxidant properties of walnut.


Assuntos
Antioxidantes/química , Juglans/química , Antioxidantes/análise , Análise dos Mínimos Quadrados , Análise Multivariada , Nozes/química , Análise de Componente Principal , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Fatores de Tempo
11.
Sci Total Environ ; 784: 147212, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-33905933

RESUMO

Dibutyl phthalate (DBP) is considered as one of the most widely used phthalate esters (PAEs), which has attracted worldwide concerns because of its potential threats to eco-environments and human health. Systematic investigations of DBP environmental occurrence contribute to the further risk assessment, which depends on effective and available analytical methods. In this study, an amplified ratiometric fluorescence ELISA was established for sensitive and high-throughput detection of DBP in the aquatic system based on a novel tetrahedral DNA nanostructure (TDN)-scaffolded-DNAzyme (Tetrazyme). Wherein, Tetrazyme was prepared by the precise folding of G-quadruplex sequence on three vertex angles of the TDN, together with hemin as the horseradish peroxidase (HRP)-mimicking enzyme. The rigid TDN avoided the local overcrowding effect to provide a reasonable spatial spacing on the interface for G-quadruplex sequence, increasing the collision chance between DNAzyme and substrates, improving the catalytic ability of DNAzyme effectively. Besides, streptavidin (SA) and biotin (bio) were used to anchor TDN and antibody, in which the specific binding of SA/bio could make more Tetrazyme conjugate on each signal element, resulting in the dual signal amplification. Meanwhile, the accuracy and precision were enhanced owing to the inherent built-in rectification to the environment from the dual output ratiometric fluorescence assay. Under the optimized conditions, the detection limit of this proposed method was 0.17 ng/mL (16 times lower than that of conventional ELISA using the same antibody) with a satisfactory accuracy (recoveries, 79.0%- 116.2%; CV, 2.1-6.5%). Overall, this platform provides a promising way for accurate, sensitive and rapid determination of DBP from environmental waters.


Assuntos
Técnicas Biossensoriais , DNA Catalítico , Dibutilftalato/isolamento & purificação , Hemina , Nanoestruturas , Poluentes Químicos da Água/isolamento & purificação , DNA , Ensaio de Imunoadsorção Enzimática , Limite de Detecção
12.
Aquat Toxicol ; 235: 105838, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33910148

RESUMO

Dibutyl phthalate (DBP) and diisobutyl phthalate (DiBP) have been reported to exhibit reproductive toxicity in vertebrates. However, the combined effect of DBP and DiBP on offspring of exposed parents remains unclear, especially for aquatic organisms such as fish. The aims of this study were to assess the effects of parental co-exposure to DBP and DiBP on early development of zebrafish offspring, and to explore the potential molecular mechanisms involved. The early developmental indicators and transcriptomic profiles of F1 larvae were examined after parental exposure to DBP, DiBP and their mixtures (Mix) for 30 days. Results showed that parental exposure to DBP and DiBP, alone or in combination, resulted in increased hatchability at 48 hpf and heart rate at 96 hpf, and increased the prevalence of malformations and mortality in F1 larvae. Generalized linear model (GLM) suggested an antagonistic interactive effect between DBP and DiBP on mortality and malformations of F1 larvae. The transcriptomic analysis revealed that the molecular mechanisms of parental co-exposure were different from those of either chemical alone. Disruption of molecular functions involved unfolded protein binding, E-box binding and photoreceptor activity in F1 larvae. These findings provide initial insights in the potential mechanism of action of parental co-exposure to DBP and DiBP.


Assuntos
Dibutilftalato/análogos & derivados , Dibutilftalato/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Perfilação da Expressão Gênica , Reprodução/efeitos dos fármacos , Transcriptoma , Peixe-Zebra/genética , Peixe-Zebra/fisiologia
13.
J Mater Chem B ; 9(14): 3136-3142, 2021 04 14.
Artigo em Inglês | MEDLINE | ID: mdl-33656045

RESUMO

Antibiotic vancomycin (Van) is often used as the drug of last resort to treat methicillin resistant Staphylococcus aureus. Due to the emergence of Van-resistant microbes, it is necessary to continuously design strategies to increase the efficacy of Van against resistant cells. In this study, an efficient method of bio-conjugating Van to bacteria is proposed using near-infrared (NIR)-light activation. A Nd3+-sensitized upconversion nanocrystal (UCNC) decorated with toluidine blue O (TB) on its surface undergoes upconverted energy transfer from the UCNC to TB when excited by 808 nm light. The photoexcited TB then catalyses the conversion of the dihydrotetrazine (dHTz) moiety in a Van-dHTz conjugate system to tetrazine which undergoes an efficient inverse electron demand Diels-Alder reaction with prior attached norbornene molecules on bacterial cell walls. The enhanced affinity of Van to bacteria by covalent bonding improves the activity of the drug against drug-resistant Enterococci, and the MIC is reduced by 6- to 7-fold as compared to neat Van. We demonstrate that the mode of action is due to increased inhibition of peptidoglycan cell wall biosynthesis. The findings in this study demonstrate that on-demand NIR-light activated bioorthogonal conjugation of Van to microbes is a viable alternative treatment in combating drug-resistant bacteria.


Assuntos
Antibacterianos/farmacologia , Compostos Aza/farmacologia , Derivados de Benzeno/farmacologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Enterococcus/efeitos dos fármacos , Raios Infravermelhos , Vancomicina/farmacologia , Antibacterianos/química , Compostos Aza/química , Derivados de Benzeno/química , Catálise , Testes de Sensibilidade Microbiana , Processos Fotoquímicos , Vancomicina/química
14.
Autophagy ; : 1-17, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33752561

RESUMO

Micronuclei are constantly considered as a marker of genome instability and very recently found to be a trigger of innate immune responses. An increased frequency of micronuclei is associated with many diseases, but the mechanism underlying the regulation of micronuclei homeostasis remains largely unknown. Here, we report that CGAS (cyclic GMP-AMP synthase), a known regulator of DNA sensing and DNA repair, reduces the abundance of micronuclei under genotoxic stress in an autophagy-dependent manner. CGAS accumulates in the autophagic machinery and directly interacts with MAP1LC3B/LC3B in a manner dependent upon its MAP1LC3-interacting region (LIR). Importantly, the interaction is essential for MAP1LC3 recruitment to micronuclei and subsequent clearance of micronuclei via autophagy (micronucleophagy) in response to genotoxic stress. Moreover, in contrast to its DNA sensing function to activate micronuclei-driven inflammation, CGAS-mediated micronucleophagy blunts the production of cyclic GMP-AMP (cGAMP) induced by genotoxic stress. We therefore conclude that CGAS is a receptor for the selective autophagic clearance of micronuclei and uncovered an unprecedented role of CGAS in micronuclei homeostasis to dampen innate immune surveillance.

15.
Carbohydr Polym ; 258: 117380, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33593586

RESUMO

A new polysaccharide from fruits of Schisandra chinensis (SCPP22) with a molecular weight of 143 ± 0.13 KDa was mainly made up of glucose and galactose. The possible structure of SCPP22 was showed that its main chain was composed of 1,4-α-d-Glup and branch was stretched from O-6 position of 1,4-ß-d-Glup. Branches consisted of T-α-d-Galp. Further, SCPP22 could reverse PCB126-induced immunosuppression, significantly enhance body weight and immune organ indices. It also significantly ameliorated oxidative injury to immune organ induced by PCB126, as shown by evaluation of SOD activities, as well as MDA levels in spleen and thymus. SCPP22 strongly stimulated cytokines production by up-regulating mRNA expression of TNF-α, INF-γ and IL-2. Mechanism investigation revealed that recovery effects of SCPP22 in immunosuppression induced by PCB126 are mainly through regulating apoptosis-related proteins expression. Schisandra polysaccharides might be applied in functional food as nutritional intervention ingredient.


Assuntos
Glucanos/química , Bifenilos Policlorados/química , Polissacarídeos/química , Schisandra/metabolismo , Animais , Peso Corporal , Feminino , Sistema Imunitário , Imunossupressão , Espectroscopia de Ressonância Magnética , Masculino , Malondialdeído/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Monossacarídeos/química , Estresse Oxidativo/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Baço/efeitos dos fármacos , Timo/efeitos dos fármacos
16.
Toxicol Lett ; 342: 26-37, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33571618

RESUMO

As a typical persistent organic pollutant, decabromodiphenyl ether (BDE-209) is associated with various health risks, especially on immune system, which is sensitive to environmental pollutants. In addition, there is a problem of multi-index estimation and lack of comprehensive evaluation in immune toxicity study. In this study, the immunotoxicity of BDE-209 was systematically estimated from the aspects of immunopathology, humoral immunity, cellular immunity and non-specific immunity, etc., and integrated biomarker responses (IBR) combined with principal component analysis was applied to comprehensively evaluate the immunotoxicity of BDE-209 and its self-recovery after discontinuation. Results showed that BDE-209 exposure could cause immunotoxicity. This response seems to depend on (1) atrophying immune organs (thymus and spleen), hepatomegaly accompanied by increasing aspartate aminotransferase and oxidative stress;(2) changing humoral (immunoglobulins) and cellular (lymphocyte proliferation and cytokine secretion) immunity indices; (3) altering related expressions of genes, and further leading to imbalance of Th1/Th2 (Th, helper T cell). Integrated biomarker responses (IBR) companied with principal component analysis selected five biomarkers (mRNA expression of GATA-3, malondialdehyde level in thymus, count of white blood cell, serum IgG and lipopolysaccharide-induced splenic lymphocyte proliferation) to clarify the immunotoxicity induced by BDE-209. Furthermore, IBR combined with factorial analysis revealed that the effect of BDE-209 could be dose-dependently reduced after withdrawal of BDE-209. Overall results suggested that BDE-209 has immunotoxicity on adult Balb/c mice, whereas this immunotoxicity could be reduced by the self-regulation of organisms to some extent.


Assuntos
Éteres Difenil Halogenados/toxicidade , Imunidade Humoral/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Baço/efeitos dos fármacos , Animais , Citocinas/genética , Citocinas/metabolismo , Esquema de Medicação , Contagem de Eritrócitos , Feminino , Retardadores de Chama/administração & dosagem , Retardadores de Chama/toxicidade , Regulação da Expressão Gênica/efeitos dos fármacos , Éteres Difenil Halogenados/administração & dosagem , Imunoglobulinas/metabolismo , Contagem de Linfócitos , Camundongos , Camundongos Endogâmicos BALB C , Baço/metabolismo
17.
Food Chem Toxicol ; 149: 112003, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33484791

RESUMO

Di-(2-ethylhexyl) phthalate (DEHP), one of the most common plasticizers, is closely associated with a high prevalence of pubertal type 2 diabetes mellitus (T2DM). Numerous studies have indicated that DEHP-induced metabolic toxicity exhibits sex differences. In this study, the sex differences in the effect of DEHP on pubertal T2DM (P-T2DM) mice, the susceptibility of female P-T2DM mice to DEHP-induced metabolic toxicity, and the underlying mechanisms were investigated. DEHP exposure exacerbated metabolic disorders in female P-T2DM mice. Factorial analysis showed that female P-T2DM mice were more sensitive to DEHP exposure than female normal mice and male P-T2DM mice. It was determined by integrated biomarker response results that female P-T2DM mice had higher risks of developing T2DM, metabolic disorders, cardiovascular events and hepatotoxicity than male P-T2DM mice. Moreover, hepatic transcriptome analysis emphasized the effects of DEHP on the expression of oxidative injury- and metabolic function-related genes. Western blotting indicated that DEHP activated Jun-N-terminal kinase (JNK) and impaired insulin sensitivity in the liver, which were the main causes of DEHP-exacerbated metabolic abnormalities in P-T2DM mice. Our study revealed that compared with normal mice and male P-T2DM mice, female P-T2DM mice tend to suffer from increased DEHP-induced metabolic toxicity, which was primarily attributed to hepatotoxicity.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/patologia , Diabetes Mellitus Tipo 2/patologia , Dietilexilftalato/toxicidade , Animais , Diabetes Mellitus Experimental , Poluentes Ambientais/toxicidade , Feminino , Masculino , Camundongos , Camundongos Endogâmicos ICR , Análise de Componente Principal
18.
Ecotoxicol Environ Saf ; 208: 111747, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396073

RESUMO

Residues of the psychoactive drug diazepam (DZP) may pose potential risks to fish in aquatic environments, especially by disrupting their behavioral traits. In this study, female and male zebrafish were subjected to chronic exposure (21 days) to sublethal doses (120 and 12 µg/L) of DZP, aimed to compare the characteristics of their behavioral responses to DZP exposure, and to investigate the possible links between those behavioral responses and variations in their brain γ-aminobutyric acid (GABA) and acetylcholinesterase (AChE) levels. Chronic exposure to DZP significantly decreased the swimming velocity and locomotor activity of both genders, indicating a typical sedative effect. Compared with males, whose locomotor activity was only significantly decreased by exposure to DZP for 21 days, females became hypoactive on day 14 (i.e., more sensitive), and they developed tolerance to the hypoactive effect induced by 120 µg/L DZP by day 21. Exposure to DZP significantly disturbed the behavioral traits related to social interactions in females but not in males. Those results indicate that DZP exhibits sex-dependent effects on the behaviors of fish. Moreover, exposure to DZP for 21 days significantly disturbed almost all of the tested behavioral traits associated with courtship when both genders were put together. Sex-dependent responses in brain GABA and AChE levels due to DZP exposure were also identified. Significant relationships between the brain GABA/AChE levels and some behavioral parameters related to locomotor activity were detected in females, but not in males.


Assuntos
Diazepam/toxicidade , Testes de Toxicidade Crônica , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/fisiologia , Acetilcolinesterase/metabolismo , Animais , Encéfalo/efeitos dos fármacos , Diazepam/administração & dosagem , Feminino , Masculino , Atividade Motora/efeitos dos fármacos , Natação , Peixe-Zebra/metabolismo , Ácido gama-Aminobutírico/farmacologia
19.
J Am Chem Soc ; 143(1): 481-487, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33356206

RESUMO

Polysulfide anions are endowed with unique redox properties, attracting considerable attentions for their applications in alkali metals-sulfur batteries. However, the employment of these anionic species in redox catalysis for small molecule synthesis remains underdeveloped due to their moderate-poor electrochemical potential in the ground state, whereas some of them are characterized by photoabsorptions in visible spectral regions. Herein, we disclose the use of polysulfide anions as visible light photoredox catalysts for aryl cross-coupling reactions. The reaction design enables single-electron reduction of aryl halides upon the photoexcitation of tetrasulfide dianions (S42-). The resulting aryl radicals are engaged in (hetero)biaryl cross-coupling, borylation, and hydrogenation in a redox catalytic regime involving S4• -/S42- and S3• -/S32- redox couples.

20.
Chem Commun (Camb) ; 56(92): 14459-14462, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33146636

RESUMO

Two Pt complexes with high quantum yields and photostability, and low cytotoxicity, were developed to track RNA G-quadruplexes (GQs) in live cells. Higher number and intensity, and longer lifetime of fluorescent foci in cancer cells than those in healthy cells suggest that the quantity and folding dynamics of RNA GQs could not only correlate to their biological functions, but be two novel biomarkers to characterize cancerous cells.


Assuntos
Biomarcadores Tumorais/análise , Complexos de Coordenação/química , DNA/química , Corantes Fluorescentes/química , Neoplasias/diagnóstico por imagem , Platina/química , Animais , Técnicas Biossensoriais , Células CHO , Bovinos , Cricetulus , Quadruplex G , Células HeLa , Humanos , Cinética , Fígado/metabolismo , Simulação de Acoplamento Molecular , Conformação de Ácido Nucleico , Imagem Óptica , RNA/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...