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1.
Food Chem ; 367: 130505, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34343813

RESUMO

Di-n-butyl phthalate (DBP) as a plasticizer is widely used in food and chemical industries. It is harm to human health when it appeared in food and water. A novel near-infrared (NIR) fluorescence molecularly imprinted sensor based on CdTe quantum dots and zeolite imidazolate framework-67 was developed with a sol-gel polymerization method for rapid and sensitive determination of DBP in foodstuff rapidly (only in 1.5 min). The fluorescence imprinted sensor provided a rapid detection method for DBP in the linear response concentration range of 0.05-18.0 µM with a low detection limit of 1.6 nM. Compared with previous fluorescence imprinted sensor, it behaved faster response speed and lower detection limit for determination of DBP. The fluorescence imprinted sensor was used to detect DBP in real samples successfully with satisfied recoveries of 97.2-106.4%, suggesting a potential application in food analysis.


Assuntos
Compostos de Cádmio , Impressão Molecular , Pontos Quânticos , Zeolitas , Dibutilftalato , Humanos , Limite de Detecção , Telúrio
2.
Pain Ther ; 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34580805

RESUMO

INTRODUCTION: Postpartum depression (PPD) is a severe psychiatric disorder that negatively affects postnatal mothers worldwide. The aim of the study is to investigate the association between postpartum pain sensitivity and PPD as well as to identify the other potential risk factors for PPD and their interconnections. METHODS: The current study was a prospective observational clinical study that enrolled 210 singleton pregnant women in a tertiary referral hospital from January to December 2020. Postpartum pain sensitivity was assessed by the Mandarin Chinese version of the Pain Sensitivity Questionnaire (PSQ), and PSQ-C-total and PSQ-C-minor scores were collected. PPD was screened by self-reporting Edinburgh Postnatal Depression Scale (EPDS), and EPDS total score was recorded. The association between postpartum pain sensitivity and PPD, as well as the risk factors and their interconnections, was analyzed by using a combination of stratified, bivariate, correlation, receiver-operating characteristic (ROC) curve, multivariate logistic regression, and path analysis. RESULTS: Overall, 87 of 210 mothers (41.4%) screened positive for PPD. A positive correlation between PSQ-C-total (r = 0.67) or PSQ-C-minor (r = 0.62) and EPDS scores was identified. PSQ-C-total [area under curve (AUC) 0.89, 95% CI 0.84-0.93; P < 0.0001] showed significantly higher diagnostic accuracy compared with PSQ-C-minor (AUC 0.85, 95% CI 0.80-0.90; P < 0.0001) in predicting EPDS scores ≥ 10 points. Multivariate logistic regression indicated that a high PSQ-C-total score [adjusted odds ratio (OR) 1.12, 95% CI 1.08-1.51; P < 0.001] and gestational diabetes (adjusted OR 2.68, 95% CI 0.96-7.47; P = 0.045) were independent risk factors, while breastfeeding (adjusted OR: 0.34, 95% CI 0.15-0.78; P = 0.007) and normal birth weight (adjusted OR 0.17, 95% CI 0.05-0.61; P = 0.0047) were independently associated with decreasing adjusted ORs for EPDS scores ≥ 10 points. The path analysis model indicated that PSQ-C-total scores and gestational diabetes showed bidirectional effects, while birth weight and breastfeeding only had a direct impact on EPDS scores ≥ 10 points. CONCLUSION: Increased postpartum pain sensitivity was closely associated with EPDS scores ≥ 10 points, and PSQ-C scores could be a reliable predictor. Moreover, gestational diabetes, low occurrence of breastfeeding, and low birth weight were the risk factors for EPDS scores ≥ 10 points. TRIAL REGISTRATION: ChiCTR.org.cn identifier, ChiCTR-2000033091.

3.
Mar Drugs ; 19(9)2021 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-34564188

RESUMO

In nature, secondary metabolites have been proven to be the essential communication media between co-occurring microorganisms and to influence their relationship with each other. In this study, we conducted a metabolomics survey of the secondary metabolites of an artificial co-culture related to a hydrothermal vent fungal-bacterial community comprising Aspergillus sclerotiorum and Streptomyces and their reciprocal relationship. The fungal strain was found to increase the secretion of notoamides and the compound cyclo(Pro-Trp) produced by the actinomycetes strain was discovered to be the responsible molecule. This led to the hypothesis that the fungi transformed cyclo(Pro-Trp) synthesized by the actinomycetes as the biosynthetic precursors of notoamides in the chemical communication. Further analysis showed Streptomyces sp. WU20 was efficient in transforming amino acids into cyclo(Pro-Trp) and adding tryptophan as well as proline into the chemical communication enhanced the induction of the notoamide accumulation. Thus, we propose that the microbial transformation during the synthetic metabolically-mediated chemical communication might be a promising means of speeding up the discovery of novel bioactive molecules. The objective of this research was to clarify the mechanism of microbial transformation for the chemical communication. Besides, this research also highlights the utility of mass spectrometry-based metabolomics as an effective tool in the direct biochemical analysis of community metabolites.

4.
J Chromatogr A ; 1655: 462487, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34487882

RESUMO

In this article, we successfully prepared three-dimensional cellulose microspheres modified by molecularly imprinted polymer for paclitaxel recognition and separation (3D-CM &PTX&MIPs). The material was characterized by Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TG) and diffraction of X-rays (XRD). Under the optimized adsorption conditions, the maximum adsorption capacity reached 65.7 mg/g. And after 5 runs of reuse, (3D-CM&PTX&MIPs) still maintained a reusability rate of 90%. Besides, (3D-CM&PTX&MIPs) showed excellent selectivity for target PTX. Finally, (3D-CM&PTX&MIPs) was used for PTX recognition and separation in the extracts of yew leaves. This research laid a good foundation and scientific basis for the efficient, environmentally friendly, and rapid enrichment of metabolites in plants using bio-based molecularly imprinted polymers.

5.
Ultrason Sonochem ; 78: 105742, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34487981

RESUMO

Traditional soaking method takes days to remove cassava cyanide. Ten minutes of ultrasonic pretreatment (UPT) was found to be a new effective method to eliminate both cyanogenic glycosides and hydrogen cyanide in cassava. Here, the parameters of UPT were optimized and the underlying mechanisms were investigated. 40.36% and 24.95% of hydrogen cyanide and cyanogenic glycosides in cassava juice were eliminated under 10 min of UPT (45℃, 81 W). UPT before boiling enhanced the total cyanide elimination to 41.94%. The degradation patterns of hydrogen cyanide and cyanogenic glycosides were different. Ultrasound directly eliminated hydrogen cyanide and indirectly degraded cyanogenic glycosides through promoting enzymatic hydrolysis. The ß-glucosidase activity was increased by 17.99% induced by ultrasound. This was supported by the movement of hydrophobic residual and the rearrangement of the secondary structure of the molecular as found in fluorescence, CD, FTIR, DSC and TG analysis. This study revealed that UPT acted as a fast and simple technical way in improving cassava safety.

6.
J Biosci Bioeng ; 132(4): 343-350, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34344604

RESUMO

Limosilactobacillusreuteri was encapsulated using Maillard-reaction-products (MRPs) of soy protein isolate (SPI) and α-lactose monohydrate by freeze-drying. The mixed solution of SPI and α-lactose monohydrate was placed in a water bath at 89°C for 160 min for Maillard reaction, and then freeze-dried to obtain MRPs. The effects of Maillard reaction on functional characteristics of MRPs and the properties of MRPs-microcapsules were studied. SDS-PAGE indicated that SPI subunit reacted with lactose to form a polymer, and the band of MRPs disappeared around the molecular weights of 33, 40, 63, and 100 kDa. Compared with SPI, the emulsion stability, emulsion activity, foaming capacity, foam stability, and gel strength of MRPs were increased by 259%, 55.71%, 82.32%, 58.53%, and 3266%, respectively. The results of Fourier transform infrared spectroscopy, circular dichroism spectroscopy, and scanning electron micrographs confirmed that the protein structure also changed significantly. Then, MRPs were used as wall material to prepare L. reuteri microcapsules. Physical properties and viable counts of L. reuteri during the simulated gastrointestinal digestion and storage period were determined. The particle size of MRPs-microcapsules (68 µm) was smaller than that of SPI-microcapsules (91 µm). The viable counts of L. reuteri in simulated gastrointestinal digestion and after storage for 30 days were improved. The modifications with Maillard reaction can improve emulsification, foaming, and gel strength of SPI, and MRPs could be used as a new type of wall material in the production of L. reuteri microcapsules.

7.
J Photochem Photobiol B ; 222: 112274, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34330082

RESUMO

Photodynamic therapy (PDT) has gained much attention in tumor therapy because of its special advantages. PDT heavily depends on the oxygen, yet the tumor microenvironment (TME) is a hypoxic and acid milieu, which weakens the PDT effect. Based on the consideration that the TME deteriorated by the PDT oxygen consumption could activate the hypoxic-sensitive small-molecule drug, we designed and prepared an integrated nanocomposite including zirconium ion metal organic framework (carrier), pyropheophorbide-a (PPa, photosensitizer), and 6-amino flavone (AF, hypoxic-sensitive drug), aiming to exert a cascaded PDT-chemotherapy (CT) antitumor effect and to solve the hypoxic challenge. The prepared nanocomposite showed great stability under the physiological (pH 7.4) condition and could continuously release PPa and AF under slightly acidic pH condition (pH 6.4), suggesting a tumor microenvironment responsive feature. Systematical in vitro and in vivo researches under various conditions (light, dark, hypoxic and normoxic) have showed that the obtained Zr-MOF@PPa/AF@PEG nanoparticles (NPs) had good biocompatibility and could achieve efficient antitumor effects based on PDT- chemotherapy (CT) cascade process. Finally, bright red fluorescence was observed in the tumor cells after internalization implying an application potential in tumor imaging.


Assuntos
Clorofila/análogos & derivados , Flavonoides/química , Estruturas Metalorgânicas/química , Nanocompostos/química , Fotoquimioterapia/métodos , Nanomedicina Teranóstica , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Clorofila/química , Clorofila/metabolismo , Clorofila/uso terapêutico , Liberação Controlada de Fármacos , Feminino , Flavonoides/metabolismo , Flavonoides/uso terapêutico , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/uso terapêutico , Nanocompostos/toxicidade , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Polietilenoglicóis/química , Oxigênio Singlete/metabolismo , Microambiente Tumoral , Zircônio/química
8.
Cancer Nurs ; 2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34310390

RESUMO

BACKGROUND: First-degree relatives of patients with colorectal cancer have an elevated risk of colorectal cancer. However, the behavior and factors potential influencing first-degree relatives regarding colorectal cancer screening in China remain unknown. OBJECTIVE: The aim of this study was to explore the screening behavior and related factors of first-degree relatives of colorectal cancer patients. METHODS: A cross-sectional design was applied, and 201 first-degree relatives participated from August 2018 to July 2019. Data were collected about demographic information, the "Colorectal Cancer Perceptions Scale," and screening behavior of first-degree relatives. Factors associated with screening behavior were identified using logistic regression analysis. RESULTS: Only 18.9% of first-degree relatives had participated in colonoscopy screening. Two Health Belief Model factors were the influencing factors of their participation in colorectal cancer screening. Higher possibility of colorectal cancer screening of first-degree relatives was associated with higher perceived susceptibility (odds ratio, 1.224; 95% confidence interval, 1.075-1.395) and lower perception of barriers (odds ratio, 0.880; 95% confidence interval, 0.820-0.944) of first-degree relatives. CONCLUSIONS: Participation in colorectal cancer screening by first-degree relatives requires improvement; perceived susceptibility and perception of barriers were the most important predictors. IMPLICATIONS FOR PRACTICE: Health professionals can enhance awareness of colorectal cancer susceptibility and address barriers to colorectal cancer screening among first-degree relatives at both individual and social levels.

9.
Bone ; 153: 116101, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34245934

RESUMO

Continuous subcutaneous insulin infusion (CSII) is an intensive insulin therapy for patients with type 2 diabetes mellitus (T2DM) who have poor glycemic control, but its effect on T2DM-related bone disorder is unclear. This study described the possible mechanisms by which CSII affects bone remodeling, structures, and mechanical properties in T2DM rats. Herein, male rats (6-week-old) were assigned randomly to 4-week and 8-week administration groups, each of which included healthy control, T2DM, CSII, and Placebo groups. Then, metabolic markers, bone formation and resorption markers in serum and protein expressions of osteoclastogenesis regulators in tibias were detected. Meanwhile, microstructures, nanostructures, macro-mechanical properties, nano-mechanical properties, and mineral compositions in femurs were evaluated. 4-week later, CSII treatment restored circulatory metabolites, bone formation and resorption markers, and osteoclastogenesis regulators, improved certain bone microstructures, decreased matrix mineralization, and increased fracture toughness in T2DM rats. For 8-week group, CSII treatment restored bone formation and resorption markers, osteoclastogenesis regulators, and bone microstructures, besides improved bone mineral compositions and nanostructures, enhanced bone mechanical properties such as fracture toughness, maximum load, elastic modulus, indentation modulus and hardness. Collectively, 8-week CSII treatment is more conducive to ameliorating bone structures and mechanical properties in T2DM rats by regulating bone remodeling compared with 4-week CSII treatment, thus improving whole bone quality and providing valuable information for clinical prevention and treatment of T2DM-related bone disorders.

10.
Chemosphere ; 283: 131179, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34146873

RESUMO

It remains unclear whether carbon content in airway macrophages (AM) can predict personal short-term exposure to fine particulate matter (PM2.5) air pollution and its respiratory health effects. We aimed to evaluate the pathway from personal PM2.5 exposure to adverse respiratory outcomes through AM carbon content. We designed a longitudinal panel study with 3 scheduled follow-ups among 113 non-smoking patients of chronic obstructive pulmonary disease in Shanghai, China, from April 2017 to January 2019. We quantified AM carbon content from induced sputum by image analysis, tested lung function and measured sputum levels of 4 pro-inflammatory cytokines and 2 anti-inflammatory cytokines. We applied the "meet in the middle" approach incorporating linear mixed-effect models to evaluate the associations from external PM2.5 exposure to respiratory outcomes through AM carbon content. Our results indicated that personal exposure to PM2.5 within 24 h was significantly associated with decreased forced expiratory volume in 1s and anti-inflammatory cytokines, as well as increased macrophages and pro-inflammatory cytokines. These changes were accompanied by increased areas of AM carbon and higher percentage of AM area occupied by carbon, both of which were associated with increased levels of pro-inflammatory cytokines and decreased levels of anti-inflammatory cytokines. Exposure to ambient black carbon and organic carbon in PM2.5 within 2 days was significantly associated with increased AM carbon area and percentage of AM area occupied by carbon. Our findings reinforced the causality in respiratory health effects of PM2.5 in which increased AM carbon content might serve as a valid exposure biomarker.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluentes Atmosféricos/toxicidade , Poluição do Ar/análise , Biomarcadores , Carbono , China , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Humanos , Macrófagos/química , Material Particulado/análise , Material Particulado/toxicidade
11.
Anal Methods ; 13(25): 2836-2846, 2021 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-34080589

RESUMO

A novel near infrared fluorescence imprinted sensor based on polyethyleneimine passivated copper-doped CdS quantum dots and zinc oxide nanorods for rapid recognition of ketoprofen was successfully prepared by sol-gel imprinting technology. The results showed that the copper-doped CdS quantum dots passivated with polyethyleneimine could improve the fluorescence lifetime and stability. Zinc oxide nanorods as carriers could improve the fluorescence response speed and sensitivity of the imprinted sensor toward ketoprofen. And the fluorescence imprinted sensor could rapidly recognize ketoprofen in just 1.0 minute. Under optimum conditions, the fluorescence intensity of the fluorescence imprinted sensor was quenched linearly by ketoprofen in the concentration range of 0.05-35.5 µM with a detection limit of 1.36 nM. The fluorescence response mechanism of the fluorescence imprinted sensor toward ketoprofen was discussed in detail, and the fluorescence quenching of the fluorescence imprinted sensor by ketoprofen was attributed to the electron transfer. The fluorescence imprinted sensor was applied to recognize ketoprofen in tap water, lake water, waste water and human urine samples rapidly with the recoveries of 97.3-103.7%. The near infrared fluorescence imprinted sensor provided a new reliable method for rapid and sensitive recognition of drugs in complex samples selectively.


Assuntos
Cetoprofeno , Impressão Molecular , Nanotubos , Pontos Quânticos , Óxido de Zinco , Humanos
12.
Aging (Albany NY) ; 13(11): 15638-15658, 2021 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-34077394

RESUMO

Dendritic cell-derived exosomes have been proven to be efficient adjuvant options for anti-tumor vaccines in cancer immunotherapy. However, their potency in atherosclerosis remains unclear. Here we summarize the association of microRNA-203-3p (miR-203-3p) with dendritic cell-derived exosomes and atherosclerosis. Firstly, dendritic cell-derived exosomes and bone marrow-derived macrophages were isolated, after which expression of miR-203-3p and cathepsin S was determined. After the establishment of atherosclerosis mouse models, gain- and loss-of-function experiments were conducted for the analysis of effects of miR-203-3p and cathepsin S on foam-cell formation, lipid accumulation, collagen deposition and serum total cholesterol. The results found high expression of cathepsin S in atherosclerosis mice and downregulation of miR-203-3p in the serum of atherosclerosis patients and ox-LDL-simulated bone marrow-derived macrophages. Cathepsin S was the target gene of miR-203-3p. miR-203-3p transporting from exosomes to bone marrow-derived macrophages resulted in inhibition of cathepsin S expression and atherosclerosis-related phenotypes in bone marrow-derived macrophages, thus alleviating atherosclerosis in mice, and this process was found to involve the p38/MAPK signaling pathway. These findings provided evidence that the transfer of miR-203-3p by dendritic cell-derived exosomes targeted cathepsin S in bone marrow-derived macrophages to attenuate atherosclerosis progression in mice, serving as a promising clinical target for atherosclerosis.


Assuntos
Aterosclerose/genética , Catepsinas/genética , Células Dendríticas/metabolismo , Regulação para Baixo/genética , Exossomos/genética , Macrófagos/metabolismo , MicroRNAs/metabolismo , Animais , Sequência de Bases , Catepsinas/metabolismo , Movimento Celular/efeitos dos fármacos , Progressão da Doença , Feminino , Inativação Gênica , Humanos , Lipoproteínas LDL/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Masculino , Camundongos Endogâmicos C57BL , MicroRNAs/genética , Pessoa de Meia-Idade , Modelos Biológicos , Fenótipo , Transporte de RNA/efeitos dos fármacos , Reprodutibilidade dos Testes
13.
Int J Biol Macromol ; 181: 1231-1242, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34022304

RESUMO

A microcrystalline cellulose-based temperature sensitivity paclitaxel molecular imprinted hydrogel (MCC-TSMIHs-PTX) was successfully prepared by temperature-sensitive monomer N-isopropylacrylamide, functional monomer 4-vinylpyridine, cross-linking agent N, N'-methylenebisacrylamide and microcrystalline cellulose. They showed imprinting effective responses to the temperature changes. The results of adsorption kinetics, adsorption equilibrium, thermodynamics, selectivity and reusability showed the successful formation of a grafting thermosensitivity hydrogel with higher adsorption capacity and specific recognition. When the temperature reached 308 K, imprinting effect of hydrogel cavities would be most effective and conducive to capture template molecules. When the temperature reached 288 K, the lowest imprinting effect would facilitate the desorption of PTX. Finally, the MCC-TSMIHs-PTX was applied to enrich the paclitaxel in Taxus × media extracts samples, the relative contents of PTX in the samples were increased greatly from 7.23% to 78.32%, indicating the MCC-TSMIHs-PTX was a stable adsorption capacity for efficient separation and enrichment of PTX in Taxus × media extracts.


Assuntos
Celulose/química , Hidrogéis/química , Impressão Molecular , Paclitaxel/farmacologia , Acrilamidas/química , Adsorção , Humanos , Cinética , Paclitaxel/química , Polímeros/química , Piridinas/química , Temperatura , Termodinâmica
14.
Fitoterapia ; 152: 104908, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33892126

RESUMO

Chemical investigation of the extracts of Aspergillus sp. CSYZ-1 resulted in the identification of compound 1, aspergillactone, a new 3,5-dimethylorsellinic acid-based meroterpenoid, together with four known metabolites (2-5). The structure and relative configuration of 1 were unambiguously determined by nuclear magnetic resonance (NMR), mass spectrometry. The absolute configuration of 1 was defined by quantum chemical TDDFT calculated and the experimental ECD spectra. The possible biosynthetic pathway of compound 1 was also proposed. The new compound exhibited potent antimicrobial activity against Helicobacter pylori and Staphylococcus aureus with MIC values of around 1-4 and 2-16 µg/mL, respectively.


Assuntos
Antibacterianos/farmacologia , Aspergillus/química , Resorcinóis/farmacologia , Terpenos/farmacologia , Antibacterianos/isolamento & purificação , China , Sedimentos Geológicos/microbiologia , Helicobacter pylori/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Resorcinóis/isolamento & purificação , Água do Mar/microbiologia , Staphylococcus aureus/efeitos dos fármacos
15.
Food Chem ; 354: 129405, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33770563

RESUMO

The intake of cassava would probably induce adverse health effects since there are toxic cyanide in cassava. However, the risk assessment of cassava consumption has not been reported in China. Therefore, this paper aimed to evaluate the dietary risks of cassava cyanide and proposed a maximum residue limit (MRL) for cyanogenic glycosides (CNGs) in cassava. The retention rate of CNGs and CN- were 61% and 11% after boiling, respectively. The acute dietary exposure of CN- and CNGs were 0.6-fold and 1.7-fold of acute risk reference dose, respectively. There was no chronic health risk across all populations concerning cassava consumption. The MRL of CNGs was proposed as 200 mg/kg in cassava. Risk assessment of cyanide for foods rich in CNGs was suggested to be based on CNGs quantification rather than that of CN-.


Assuntos
Cianetos/análise , Exposição Dietética , Manihot/química , China , Cromatografia Líquida de Alta Pressão , Culinária , Glicosídeos/análise , Humanos , Manihot/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Medição de Risco , Espectrometria de Massas em Tandem
16.
J Med Genet ; 2021 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-33563768

RESUMO

PURPOSE: Universal germline testing in patients with colorectal cancer (CRC) with a multigene panel can detect various hereditary cancer syndromes. This study was performed to understand how to choose a testing panel and whether the result would affect clinical management. METHODS: We prospectively enrolled 486 eligible patients with CRC, including all patients with CRC diagnosed under age 70 years and patients with CRC diagnosed over 70 years with hereditary risk features between November 2017 and January 2018. All participants received germline testing for various hereditary cancer syndromes. RESULTS: The prevalence of germline pathogenic variants (PVs) in cancer susceptibility genes was 7.8% (38/486), including 25 PVs in genes with high-risk CRC susceptibility (the minimal testing set) and 13 PVs in genes with moderate-risk CRC susceptibility or increased cancer risk other than CRC (the additional testing set). All the clinically relevant PVs were found in patients diagnosed under age 70 years. Among them, 11 patients would not have been diagnosed if testing reserved to present guidelines. Most (36/38) of the patients with PVs benefited from enhanced surveillance and tailored treatment. PVs in genes from the minimal testing set were found in all age groups, while patients carried PVs in genes from the additional testing set were older than 40 years. CONCLUSION: Universal germline testing for cancer susceptibility genes should be recommended among all patients with CRC diagnosed under age 70 years. A broad panel including genes from the additional testing set might be considered for patients with CRC older than 40 years to clarify inheritance risks. TRIAL REGISTRATION NUMBER: NCT03365986.

17.
Sleep Breath ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33619665

RESUMO

BACKGROUND: Sleep deprivation (SD) has become a serious concern worldwide. This study aimed to identify key modules and candidate hub genes correlated with diseases caused by SD, using co-expression analysis. METHODS: The weighted gene co-expression network analysis was performed to construct a co-expression network of hub genes correlated with SD. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed to search for signaling pathways. The protein-protein interaction network analysis of central genes was performed to recognize the interactions among central genes. Molecular Complex Detection, a plugin in Cytoscape, was used to discover the hub gene clusters involved in SD. RESULTS: A total of 564 genes in the yellow module were identified based on the results of topological overlap measure-based clustering. The yellow module showed a pivotal correlation with SD. Six hub gene clusters prominently associated with SD were identified. Heat shock protein family and circadian clock genes among them may be the hub genes involved in SD. CONCLUSIONS: These genes and pathways might become therapeutic targets with clinical usefulness in the future.

18.
Respir Res ; 22(1): 58, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33596919

RESUMO

BACKGROUND: An adverse role for obstructive sleep apnea (OSA) in cancer aggressiveness and mortality has recently emerged from clinical and animal studies, and the reasons have not been fully determined. Cancer stem cells (CSCs) are regarded as the main cause of carcinoma metastasis. So far, the relationship between OSA and lung CSCs has not been explored. METHOD: In the present study, we established an orthotopic mouse model of primary lung cancer and utilized chronic intermittent hypoxia (CIH) exposure to mimic OSA status. RESULTS: We observed that CIH endows lung cancer with greater metastatic potential, evidenced by increased tumor growth, tumor seeding, and upregulated CSC-related gene expression in the lungs. Notably, the transcription factor BTB and CNC homology 1 (Bach1), a key factor in responding to conditions of oxidative stress, is increased in lung cancer after CIH exposure in vitro and in vivo. Meanwhile, exposing lung cancer cells to CIH promoted cell proliferation, clonal diversity, induced stem-like cell marker expression, and gave rise to CSCs at a relatively higher frequency. Furthermore, the increase of mitochondrial ROS (mtROS) and CSC-marker expression induced by CIH exposure was abolished in Bach1 shRNA-treated lung cancer cells. CONCLUSIONS: Our results indicated that CIH promoted lung CSC-like properties by activating mtROS, which was partially mediated by Bach1.

19.
Bioresour Technol ; 323: 124607, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33385629

RESUMO

The high cost of carbon source limits the heterotrophic culture of Chlorella. In this study, broken rice was hydrolyzed into glucose. Then, the broken rice hydrolysate (BRH) was utilized for heterotrophic cultivation of C. vulgaris instead of glucose. Results showed that algal cells released H+ when they consumed NH4+, leading to a sharp decrease in pH. Growth inhibition by acid could be avoided by using a pH buffer. Adding alkaline reagents intermittently during culture could not only reduce the amount of pH stabilizer but also obtain increased biomass production. When using Tris as pH stabilizer, the biomass productivity of C. vulgaris in BRH was the largest (1.01 g/L/d), followed by NaOH (1.00 g/L/d), and Na2CO3 (0.95 g/L/d). Using BRH instead of glucose for heterotrophic cultivation of C. vulgaris could save 89.58% of the cost of culture medium. This study developed a novel strategy for cultivating C. vulgaris heterotrophically using BRH.


Assuntos
Chlorella vulgaris , Oryza , Biomassa , Carbono , Processos Heterotróficos
20.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(1): 92-95, 2021 Jan 10.
Artigo em Chinês | MEDLINE | ID: mdl-33423268

RESUMO

OBJECTIVE: To explore the effect and precautions of setting up a genetic clinic for hereditary colorectal cancers. METHODS: To collect the information of the patients who received genetic screening and genetic counseling at our hospital from January 2016 to June 2018, and analyze the role of family history collection and follow-up management. RESULTS: The detection rate of family history of tumors has increased by 13.6%. Follow up management was carried out in 156 families with hereditary colorectal cancer confirmed by detection of germline mutations. Five cases of early colorectal cancers and 12 cases of adenomatous polyps were detected and treated. CONCLUSION: To set up genetic clinic is helpful to standardize the management of high-risk population, and attention should be paid to the role of family history collection and follow-up management.


Assuntos
Neoplasias Colorretais , Testes Genéticos , Neoplasias Colorretais/genética , Neoplasias Colorretais/prevenção & controle , Neoplasias Colorretais/cirurgia , Aconselhamento Genético , Testes Genéticos/normas , Mutação em Linhagem Germinativa , Humanos , Fatores de Risco
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