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1.
Int J Hyperthermia ; 37(1): 37-48, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31918588

RESUMO

Purpose: In cardiac radiofrequency (RF) ablation, RF energy is often used to create a series of transmural lesions for blocking accessory conduction pathways. Electrode-tissue contact force (CF) is one of the key determinants of lesion formation during RF ablation. Low electrode-tissue CF is associated with ineffective RF lesion formation, whereas excessive CF may increase the risk of steam pop and perforation. By using finite element analysis, we studied lesion size and features at different values of electrode-tissue CF in cardiac RF ablation.Materials and methods: A computer-model-coupled electrode-tissue CF field, RF electric field, and thermal field were developed to study temperature distribution and lesion dimensions in cardiac tissue subjected to CF of 2, 5, 10, 20, 30, and 40 g with identical RF voltage and duration.Results: Increasing CF was associated with an increase in lesion depth, width, and cross-section area. The lesion cross-section area exhibited a linear increase, and the lesion width was significantly greater than lesion depth under the identical ablation condition. The relationship between CF value and lesion size is a power function: Lesion Size = a × CFb (Lesion Depth = 3.17 × CF0.14 and Lesion Width = 5.17 × CF0.14).Conclusions: This study confirmed that CF is a major determinant of RF lesion size and that electrode-tissue CF affects the amount of power dissipated in tissue. At a constant RF voltage and application time, RF lesion size increases as CF increases.

2.
FASEB J ; 33(11): 12760-12767, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31480859

RESUMO

Chemotherapy resistance is one of the most common causes of death among patients with ovarian cancer, and identifying novel antitumor agents is a priority. Here, we report that the novel molecule 2-(anaphthoyl)ethyltrimethylammonium iodide (α-NETA) induces epithelial ovarian cancer (EOC) cell pyroptosis through the gesdermin-d (GSDMD)/caspase-4 pathway. Furthermore, Cell Counting Kit-8 fluorescence-activated cell sorting analysis showed that α-NETA treatment led to cell death in different ovarian cancer cell lines, including Ho8910, Ho8910PM, and A2780. Morphologic examination by electron microscopy indicated that cells treated with α-NETA produced multiple microbubbles, typical of cells undergoing pyroptosis. α-NETA also significantly increased expression of pyroptosis-associated molecules including caspase-4 and GSDMD in EOC cells. Knockdown of either caspase-4 or GSDMD in ovarian cancer cells strongly interfered with α-NETA cell-killing activity, indicating that α-NETA acts through the pyroptosis pathway. In vivo, α-NETA treatment dramatically decreased the size of EOC tumors in mice. Our findings suggest that α-NETA represents a potential new antitumor molecule or lead compound for EOC chemotherapy.-Qiao, L., Wu, X., Zhang, J., Liu, L., Sui, X., Zhang, R., Liu, W., Shen, F., Sun, Y., Xi, X. α-NETA induces pyroptosis of epithelial ovarian cancer cells through the GSDMD/caspase-4 pathway.

3.
Nature ; 572(7769): 341-346, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31367039

RESUMO

Salinity is detrimental to plant growth, crop production and food security worldwide. Excess salt triggers increases in cytosolic Ca2+ concentration, which activate Ca2+-binding proteins and upregulate the Na+/H+ antiporter in order to remove Na+. Salt-induced increases in Ca2+ have long been thought to be involved in the detection of salt stress, but the molecular components of the sensing machinery remain unknown. Here, using Ca2+-imaging-based forward genetic screens, we isolated the Arabidopsis thaliana mutant monocation-induced [Ca2+]i increases 1 (moca1), and identified MOCA1 as a glucuronosyltransferase for glycosyl inositol phosphorylceramide (GIPC) sphingolipids in the plasma membrane. MOCA1 is required for salt-induced depolarization of the cell-surface potential, Ca2+ spikes and waves, Na+/H+ antiporter activation, and regulation of growth. Na+ binds to GIPCs to gate Ca2+ influx channels. This salt-sensing mechanism might imply that plasma-membrane lipids are involved in adaption to various environmental salt levels, and could be used to improve salt resistance in crops.

4.
Curr Mol Med ; 19(9): 699-703, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31362674

RESUMO

OBJECTIVE: This study aimed to explore the clinical effect of levetiracetam in the treatment of children with epilepsy. METHODS: 136 children with epilepsy were selected from January 2017 to December 2017. According to the random number table method, they were divided into the experimental group and the conventional group, with 68 cases in each group. The conventional group was treated with valproate, while the experimental group was treated with levetiracetam. The effective rate, the cognitive function and the frequency of clonic seizures in the two groups were compared. RESULTS: There was no significant difference in the total effective rate between the two groups (P>0.05). There was no significant difference in attention, executive ability, abstract and orientation scores between the two groups before treatment (P>0.05). After treatment, the focus of attention (106.54±6.56), executive ability (105.76±6.77), abstract and directional score (106.65±6.57) were significantly higher than that of the conventional group. The difference in the two groups was statistically significant (P<0.05). After 3 months of treatment, the frequency of myoclonic seizures (9.22±0.95) and the frequency of tonic-clonic seizures (11.68±1.36) were found to be significantly lower than those of the conventional group, and the difference between the two groups was statistically significant (P<0.05). CONCLUSION: Levetiracetam is effective in the treatment of children with epilepsy. It can effectively improve the cognitive function of the patients, reduce the frequency of myoclonic seizures and tonic-clonic seizures, and has a high promotion value.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31295128

RESUMO

Atrial fibrillation (AF) is the most frequently occurring form of arrhythmia, which induces multiple fatal diseases and impairs the quality of life in patients; thus, the study of the diagnostic methods for detecting AF is clinically important. Here we present a feature extraction method for the detection of AF using a ballistocardiogram (BCG), which is based on a physiological signal database collected by a non-contact sensor. The BCG signals, including both with AF and sinus rhythm (SR), were collected from 37 subjects during overnight sleep (approximately 8 hours). The signals were split into 2915 one-minute segments (AF: 1494, SR: 1421) without overlap and labeled as AF and SR. BCG signals were transformed into BCG energy signals in order to highlight the features of AF and SR BCG signals; and four new data sequences representing different characteristics of the BCG energy signals were generated. The mean value, variance, skewness, and kurtosis of the four data sequences were calculated and 16 features were extracted for each segment. Five machine learning algorithms were used for classification. The results of this study show that the support vector machine (SVM) performed the best among the five tested classifiers and achieved sensitivity, precision, and accuracy of 0.968, 0.928, and 0.945, respectively. These results indicate that the proposed feature extraction method can be well applied to AF and SR classification and may lay foundations for the development of systems for long-term home cardiac monitoring and AF screening.

6.
Biomed Pharmacother ; 117: 109141, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31228800

RESUMO

Millettia pulchra Kurz var-laxior (Dunn) Z. Wei, a wild-growing plant of the family Fabaceae is known to possess multifarious medicinal properties. 17-Methoxyl-7-hydroxy-benzene-furanchalcone (MHBFC) is a flavonoid monomer extracted from its root, which has been used in traditional Chinese medicine, with a long history as a remedy of hypertension and cardiovascular remodeling. The present study was conducted to further investigate the regulatory mechanisms of MHBFC based on the endothelial nitric oxide synthase-nitric oxide (eNOS-NO) signaling pathway. The abdominal aorta of the male Sprague-Dawley rats was narrowed to induce cardiac remodeling, and the rats were given corresponding drugs for 6 weeks after operation. At the end of the experiment, the relevant indexes were detected. The results showed that Nω-nitro-L-arginine methyl ester (L-NAME) could increase the myocardial cell cross-section area, myocardial fibrosis, and the cardiac collagen volume fraction. The serum NO and eNOS levels and the expression of p-eNOS, p-PI3K and p-Akt protein were decreased, and myocardial microvascular endothelial cell (MMVEC) apoptosis increased. However, the above changes were reversed after treatment with MHBFC. These results indicated that MHBFC could increase eNOS protein phosphorylation by increasing PI3K and Akt protein phosphorylation, and activated the eNOS-NO signaling pathway, increased eNOS enzyme activity, catalyzed the generation of protective NO, and counteracted MMVEC apoptosis induced by cardiac remodeling, thereby protecting against myocardial damage and reversing cardiac remodeling.

7.
G3 (Bethesda) ; 9(8): 2497-2509, 2019 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-31208958

RESUMO

Pearl millet is an important food crop in arid and semi-arid regions of South Asia and sub-Saharan Africa and is grown in Australia and the United States as a summer fodder crop. The d2 dwarf germplasm has been widely used in the last half-century to develop high-performing pearl millet hybrids. We previously mapped the d2 phenotype to a 1.6 cM region in linkage group (LG) 4 and identified the ABCB1 gene as a candidate underlying the trait. Here, we report the sequence, structure and expression of ABCB1 in tall (D2D2) and d2 dwarf (d2d2) germplasm. The ABCB1 allele in d2 dwarfs differs from that in tall inbreds by the presence of two different high copy transposable elements, one in the coding region and the second located 664 bp upstream of the ATG start codon. These transposons were present in all d2 dwarfs tested that were reported to be of independent origin and absent in the analyzed wild-type tall germplasm. We also compared the expression profile of this gene in different organs of multiple tall and d2 dwarf inbreds, including the near-isogenic inbreds at the d2 locus, Tift 23B (D2D2) and Tift 23DB (d2d2). Heterologous transformation of the tall (Ca_ABCB1) and the d2 dwarf (Ca_abcb1) pearl millet alleles in the Arabidopsis double mutant abcb1abcb19 showed that the pearl millet D2 but not the d2 allele complements the Arabidopsis abcb1 mutation. Our studies also show the importance of the COOH-terminal 22 amino acids of the ABCB1 protein in either protein function or stability.

8.
Onco Targets Ther ; 12: 3779-3790, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31190878

RESUMO

Background: In recent years, a large number of studies have shown that differentially expressed lncRNAs are capable of promoting the occurrence and development of tumors by regulating cell proliferation and differentiation. However, the biological effects of lncRNAs in non-small cell lung cancer (NSCLC) are still needed to be further investigated. Methods: The differentially expressed lncRNAs in NSCLC tissues in the downloaded profiles from GEO database were analyzed and further verified in 100 pairs of NSCLC samples collected in our hospital. After identification of the target gene MIR210HG, the relationship between MIR210HG expression and clinical data of NSCLC patients was analyzed. Regulatory effects of MIR210HG on proliferation, migration, and invasion of NSCLC cells were detected by CCK-8, colony formation, and transwell assay, respectively. The binding condition of MIR210HG and DNA methyltransferase 1 (DNMT1) was detected by RNA binding protein immunoprecipitation. Subsequently, chromatin immunoprecipitation assay assessed the promoter binding of DNMT1 to CACNA2D2. Rescue experiments were conducted to assess whether CACNA2D2 can reverse the function of MIR210HG. Results: MIR210HG was highly expressed in NSCLC tissues not only in GSE30219 dataset but also in our collected NSCLC tissues. MIR210HG expression was correlated to tumor stage and lymph node metastasis of NSCLC patients. Besides, lower disease-free survival (DFS) and overall survival (OS) were found in NSCLC patients with high-level MIR210HG compared with those with low-level MIR210HG. Regression analysis indicated that MIR210HG was the independent risk factor for DFS and OS of NSCLC patients. In vitro experiments demonstrated that MIR210HG knockdown remarkably inhibited proliferation and migration of NSCLC cells. MIR210HG could recruit DNMT1, thereafter promoting methylation of CACNA2D2 promoter region. CACNA2D2 overexpression remarkably inhibited cell proliferation. Moreover, inhibited proliferation induced by MIR210HG knockdown was reversed by CACNA2D2 knockdown. Conclusion: MIR210HG can promote the tumorigenesis of NSCLC by inhibiting the expression of CACNA2D2. Our findings provide new therapeutic strategies for the future treatment of NSCLC.

9.
Front Neurol ; 10: 539, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31178819

RESUMO

Background and Purpose: The incidence and prevalence of autoimmune encephalitis is gradually increasing. This retrospective observational study primarily aimed to analyze the clinical characteristics of autoimmune encephalitis patients in the Second Xiangya Hospital and report patient prognoses after immunotherapy. Methods: The clinical data of 86 patients who were diagnosed with autoimmune encephalitis from October 2014 to September 2018 were collected, and their corresponding clinical characteristics, laboratory examination, treatment, and outcome data analyzed. Results: In our study, 72 patients (83.7%) were positive for anti-NMDAR (N-methyl-D-aspartate receptor) antibody; 5 patients (6%) for anti-GABABR (γ-aminobutyric acid receptor-A); 4 patients (4.7%) for anti-LGI1 (leucine-rich, glioma inactivated 1); 3 patients (3.5%) for anti-Caspr2 (contactin-associated protein-like 2) (1 patient was positive for both anti-LGI1 and anti-Caspr2 antibodies); and 3 patients (3.5%) for onconeural antibodies. Among the 86 patients diagnosed as having autoimmune encephalitis, 50% showed acute disease onset (≤2 weeks). The most common inducing factor was fever or cold (17/86, 19.8%). The main clinical symptoms included, among others, psychiatric disturbances (82.5%), epilepsy (60.5%), autonomic dysfunction (58.1%), sleep disorders (45.3%), consciousness disorders (45.3%), and speech disorders (46.5%). No significant correlation between ICU admission rates and CSF or serum antibody scores was observed. However, CSF antibody scores of (+ + +) and (++) were associated with longer lengths of hospitalization (p < 0.05) and a higher CSF WBC count when compared with CSF antibody scores of (+) in patients with anti-NMDAR encephalitis (p < 0.05). Additionally, there was no significant correlation between mRS score difference on admission and discharge (after immunotherapy) and age, sex, and choice of immune treatment, while immune therapy taken within 15 days from onset was more inclined to be associated with an mRS score difference ≥2 after immunotherapy in patients with anti-NMDAR encephalitis (p = 0.006). Conclusions: Autoimmune encephalitis has an acute or sub-acute onset and presents with psychotic symptoms, epilepsy, and autonomic dysfunction. The sex ratio in anti-NMDAR encephalitis was nearly balanced. Infection was a major factor inducing anti-NMDAR encephalitis, and the CSF antibody scores could be helpful in determining its prognosis since these scores showed associations with hospitalization duration and CSF WBC counts.

10.
J Cancer ; 10(7): 1616-1621, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31205517

RESUMO

Estrogen plays an essential role in type I endometrial cancer cell proliferation. Despite great progresses in the etiology has been obtained in the past, however, the molecular mechanisms remain to be fully clarified. Prohibitin has been demonstrated involvement in multiple cancers' development. If it also contributes to estrogen-driven endometrial cancer proliferation is not clear. IHC assay result display that prohibitin overexpressed in endometrial cancer tissue and associated with the poor prognosis; Western blot assay detect that upregulated prohibitin expression with dose- and time-dependent manners. The cellular growth was monitored with SRB assay which demonstrate that knockdown prohibitin attenuated estrogen-induced proliferation. Ubiquitination assay finds estrogen increased prohibitin level through stabilizing prohibitin protein via inhibition of ubiquitination, while estrogen-induced protein expression was mediated by estrogen receptor. Our findings provide a new insight on the mechanism of estrogen-induced proliferation, implying the possibility of using prohibitin as a potential therapeutic target for the treatment of endometrial cancer.

11.
Onco Targets Ther ; 12: 4167-4179, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31213835

RESUMO

Background: Aberrant expression of microRNAs (miRNAs) in non-small-cell lung cancer (NSCLC) has been reported. Dysregulation of miRNAs exerts tumor-suppressing or tumor-promoting actions on the pathology and biological behaviors of NSCLC. miR-612 is associated with many types of human cancer; however, the expression, potential roles, and regulatory mechanisms of miR-612 in NSCLC remain unclear. Material and methods: Here, the expression level of miR-612 in NSCLC tissue specimens and a panel of cell lines were evaluated by RT-qPCR. Cell-Counting Kit 8, flow cytometry, Transwell migration and invasion, and in vivo tumor growth assays were performed to determine the functional role of miR-612 in malignant phenotypes of NSCLC cells. The molecular mechanism underlying the tumor-suppressive roles of miR-612 in NSCLC was investigated. Results: miR-612 was expressed at low levels in NSCLC, and low miR-612 expression was significantly correlated with TNM stage and lymph node metastasis. NSCLC patients with low miR-612 expression had shorter overall survival rate than those with high levels. Exogenous miR-612 expression decreased proliferation, migration, and invasion, and promoted apoptosis of NSCLC cells in vitro. miR-612 upregulation hindered NSCLC tumor growth in vivo. Bromodomain-containing protein 4 (BRD4) was confirmed as a direct target gene of miR-612 in NSCLC cells. BRD4 was obviously overexpressed in human NSCLC tissues and inverse correlated with miR-612 expression. Inhibition of BRD4 expression simulated the tumor-suppressive functions of miR-612 overexpression in NSCLC cells. Reintroduction of miR-612 expression abrogated the miR-612-mediated suppressive effects on NSCLC cells. BRD4 upregulation inhibited activation of the PI3K/Akt pathway in NSCLC cells in vitro and in vivo. Conclusion: This study supports the first evidence that miR-612 exerts tumor-suppressive roles in the aggressive behaviors of NSCLC cells in vitro and in vivo through direct targeting BRD4 and deactivating the PI3K/Akt pathway. Thus, miR-612 might be a promising target for anticancer therapies in patients with NSCLC.

12.
Cardiovasc Intervent Radiol ; 42(8): 1183-1191, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31044293

RESUMO

PURPOSE: To evaluate the level of artery occlusion, degradation periods, tissue response and vessel recanalization of calibrated gelatin sponge particles after segmental renal artery embolization. MATERIALS AND METHODS: Superselective embolization of 14 adult rabbits was performed with calibrated gelatin sponge particles (150-350 µm). Two rabbits were killed immediately after the procedure (day 0). One pair of rabbits was killed on each of the following days: 1, 3, 7, 14, 28 and 56. One rabbit from each pair underwent CT angiography before embolization and killing. The pathologic changes of the embolized renal parenchyma and embolic characteristics of calibrated gelatin sponge particles were evaluated histologically and angiographically. RESULTS: Calibrated gelatin sponge particles were distally located in interlobular artery with a dense packing on day 0. The level of occlusion paralleled the size of the particles. Partial degradation of the particles was observed on day 3, and complete degradation was observed on day 14. Vessel recanalization was observed through both CTA and histological analysis starting on day 3. Vascular inflammation responding to gelatin sponge particles was mild and subsided with the degradation of the particles. On day 28 and day 56, attenuation of embolized vessels occurred due to marked intimal proliferation, and vascular occlusion developed. CONCLUSIONS: Gelatin sponge particles of 150-350 µm produced dense and distal embolization, and were resorbed before day 14 with a mild tissue reaction. Vessel recanalization occurred secondary to the resorption of gelatin sponge particles, but permanent vascular occlusion developed due to marked intimal hyperplasia after day 28.


Assuntos
Embolização Terapêutica/instrumentação , Embolização Terapêutica/métodos , Esponja de Gelatina Absorvível/uso terapêutico , Artéria Renal/patologia , Animais , Calibragem , Angiografia por Tomografia Computadorizada , Gelatina , Modelos Animais , Coelhos , Artéria Renal/diagnóstico por imagem
13.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(3): 244-250, 2019 Mar 28.
Artigo em Chinês | MEDLINE | ID: mdl-30971515

RESUMO

OBJECTIVE: To investigate the effects of different wavelet filters on correlation and diagnostic performance of radiomics features.
 Methods: A total of 143 colorectal cancer (CRC) patients (64 positive in lymph node metastasis and 79 negative) with contrast-enhanced CT examination were recruited. After labeling the tumor area by experienced radiologists, radiomics wavelets features based on 48 different wavelets were extracted using in-house software coded by Matlab. The correlation coefficients of the features with same names between different wavelets were calculated and got the distribution of high-correlation features between each wavelet. The least absolute shrinkage and selection operator (LASSO) was used to build signatures between lymph node metastasis and wavelet features data set based on different wavelets. The numbers of features in signatures and diagnostic performance were compared using Delong's test.
 Results: With the difference of wavelet order increased, the number of high-correlation features between two wavelets decreased. Some features were prone to high correlation between different wavelets. When building radiomics signature based on single wavelet, signatures built from 'rbio2.2', 'sym7' and 'db7' did well in predicting lymph node metastasis. The signature based on Daubechies wavelet feature set had the highest performance in predicting lymph node metastasis, while the signature from Biorthogonal wavelet features was worst. Improvement was significant in diagnostic performance after excluding the high-correlation features in the whole features set (P=0.004).
 Conclusion: In order to reduce the data redundancy of features, it is recommended to select wavelets with large differences in wavelet orders when calculating radiomics wavelet features. It is necessary to remove high correlation features for improving the diagnostic performance of radiomics signature.


Assuntos
Neoplasias Colorretais , Humanos , Metástase Linfática , Estudos Retrospectivos
14.
Neurochem Int ; 128: 14-20, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30930273

RESUMO

Regulated in development and DNA damage responses 1 (REDD1) is a highly conserved stress-response protein and can be induced by hypoxia/ischemia and DNA damage. However, it is not known whether REDD1 involves in neuronal damage caused by subarachnoid hemorrhage (SAH) that is known as one of the most important causes of disability and death worldwide. Here, we first found that SAH markedly induced the increase of REDD1 (35.467 ng/ml) in cerebrospinal fluid (CSF) of patients at acute stage (within 24 h from bleeding) compared to that of control (0.644 ng/ml). And, REDD1 level was positively correlated with severity of brain injuries (Hunt-Hess grade of SAH), but it showed an obvious decline at recovery stage 6.201 ng/ml (before discharge from hospital) because of good recovery. Moreover, it was found that the expression of REDD1 was significantly induced by hemolysate in a dose-dependent way in neurons. Knockdown of REDD1 by lentivirus encoded REDD1-shRNA could inhibit the neuronal apoptosis and LDH leakage caused by hemolysate. Importantly, the level of REDD1 in peripheral blood of SAH patients was significantly higher (4.364 ng/ml) than that of healthy persons (1.317 ng/ml) and also was positively correlated with that in CSF. Taken together, our findings provide the novel and direct evidence that REDD1 could play a critical role of process of neuronal damage caused by SAH, suggesting a new molecular target to protect brain function from SAH injury.

15.
Acc Chem Res ; 52(4): 867-875, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30865425

RESUMO

Access to safe drinking water is among the 17 United Nations sustainable development goals. As the largest developing country, China has confronted large challenges to providing safe and sufficient drinking water to its population of 1.4 billion under the conditions of limited water sources and ubiquitous water contamination. This Account outlines these challenges as well as the practical solutions implemented by Chinese water professionals. We first provide a general introduction of the water supply in China. Next, we describe the main challenges of water source shortages and source water contamination. The practical solutions developed by Chinese water professionals are the core part of this Account, to which we have devoted ourselves to and contributed in some issues and cases. The water supply in China is a binary system that reflects the gap between urban and rural communities. Both urban and rural water supplies have been subject to water source shortages and contamination. Water shortages are mainly solved by long-distance water transportation projects. Urban water utilities generally pay attention to organic matter, ammonia, algae, and chemical spills in source water while also focusing on micro-organisms and disinfection byproducts in tap water. Micro-organisms are a widespread concern for rural water supplies, whereas arsenic, fluoride, and ammonia are an endemic concern in some rural communities. Investment in updating of treatment processes significantly benefits urban water supplies, and advanced treatment of ozonation and biologically activated carbon processes are now commonly used to ensure that strict drinking water quality standards are met. However, this is not the case for rural water supplies, where expensive advanced treatment is not affordable. Thus, improving rural water supplies requires approaches such as searching for sources with better water quality, using automated ultrafiltration instruments, or connecting to urban water supply distribution systems. For rural areas with high concentrations of arsenic or fluoride in source water, specific adsorbents are a practical way to help farmers. Similar challenges will be encountered elsewhere in the world; therefore, the practical solutions applied in China will be useful to other countries in different stages of development.

16.
IEEE Trans Biomed Circuits Syst ; 13(2): 314-329, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30640626

RESUMO

Nowadays, cardiovascular disease is still one of the primary diseases that limit life expectation of humans. To address this challenge, this work reports an Internet of Medical Things (IoMT)-based cardiovascular healthcare system with cross-layer optimization from sensing patch to cloud platform. A wearable ECG patch with a custom System-on-Chip (SoC) features a miniaturized footprint, low power consumption, and embedded signal processing capability. The patch also integrates wireless connectivity with mobile devices and cloud platform for optimizing the complete system. On the big picture, a "wearable patch-mobile-cloud" hybrid computing framework is proposed with cross-layer optimization for performance-power trade-off in embedded-computing. The measurement results demonstrate that the on-patch compression ratio of the raw ECG signal can reach 12.07 yielding a percentage root mean square variation of 2.29%. In the test with the MIT-BIH database, the average improvement of signal to noise ratio and mean square error are 12.63 dB and 94.47%, respectively. The average accuracy of disease prediction operation executed in cloud platform is 97%.

17.
Int J Gynecol Cancer ; 29(1): 23-27, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30640679

RESUMO

OBJECTIVE: To evaluate the use of a portable, rechargeable colposcope combined with human papillomavirus (HPV) testing, as compared with HPV testing alone, for screening of cervical cancer and pre-cancerous lesions. METHODS: This was a cross-sectional study among 488 women in Baoshan County, Yunnan. The women underwent HPV testing followed by Gynocular portable colposcopy with visual inspection with acetic acid. Obvious lesions were biopsied. If portable colposcopy testing was negative but HPV testing was positive, the women underwent follow-up testing with thin-prep cytology and traditional colposcopy. Cervical biopsies were performed for any abnormalities. Histopathology was followed up with diagnosis and treatment. RESULTS: Among 488 women screened with portable colposcopy, 24 women underwent biopsy based on positive colposcopy screening. Of these 24 women, three were HPV positive and 21 were HPV negative. Five women had cervical intra-epithelial neoplasia (CIN) I and one had advanced cervical cancer. Forty-six women tested positive for HPV. Three of these women had screened positive on preliminary colposcopy, with one positive for CIN III/squamous cell carcinoma and one woman with CIN I. Forty-three women underwent follow-up testing with thin-prep cytology. Two women had atypical squamous cells of undetermined significance and five had low-grade squamous intra-epithelial lesions and were biopsied; three women had CIN I, one had CIN II and one had CIN III. HPV testing and portable colposcopy was more sensitive but slightly less specific than portable colposcopy or HPV testing alone. CONCLUSION: While HPV testing has high sensitivity and specificity for the detection of pre-cancerous and cancerous lesions and portable colposcopy has lower specificity, both methods of detection have low positive predictive value and high negative predictive value. In tandem, HPV testing and portable colposcopy had higher sensitivity for detection among women who underwent biopsies. In clinical practice, portable colposcopy was an effective, easy and affordable tool to transport to villages where cytology is not currently feasible.

18.
Chemosphere ; 221: 333-341, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30641374

RESUMO

Microplastics in the environment may gain entry the human gastrointestinal tract through the food chain. However, information on different adverse effects of microplastics at nanometer or micrometer scales in human intestine cells is limited. This study compared the cytotoxicity and efflux pump inhibition ability of 0.1 µm and 5 µm polystyrene microplastics (PS-MPs) in the human colon adenocarcinoma Caco-2 cells. Both PS-MP sizes exhibited low toxicity on cell viability, oxidative stress, and membrane integrity and fluidity. However, the mitochondrial membrane potential was disrupted by both sizes of PS-MPs, and the 5 µm PS-MPs induced higher effects than 0.1 µm PS-MPs. Furthermore, 0.1 µm (≥20 µg/mL) or 5 µm (≥80 µg/mL) PS-MPs inhibited plasma membrane ATP-binding cassette (ABC) transporter activity and increased arsenic (one substrate of ABC transporter) toxicity. The 0.1 µm PS-MPs might act as substrates of ABC transporter to reduce the transport capacity of other substrates. However, high concentrations of 5 µm PS-MPs might reduce ABC transporter activity through induction of mitochondrial depolarization and potential depletion of ATP. This study provides basic information on the toxicity of 0.1 µm and 5 µm PS-MPs in human intestine cells, which are useful for assessing the risk of PS-MPs in humans.


Assuntos
Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores , Plásticos/toxicidade , Transportadores de Cassetes de Ligação de ATP/metabolismo , Células CACO-2 , Sobrevivência Celular/efeitos dos fármacos , Humanos , Intestinos/patologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Plásticos/metabolismo , Poliestirenos/metabolismo , Poliestirenos/toxicidade , Medição de Risco
20.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 47(4): 329-337, 2018 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-30511517

RESUMO

OBJECTIVE: To observe and verify the key anatomies of DeLancey's three levels of vaginal support theory through laparoscopic surgery by space dissection technique. METHODS: The features and stress performance of related anatomies were observed and analyzed in laparoscopic type C hysterectomy and pelvic lymphadenectomy for cervical cancer by natural space exposures. RESULTS: The main ligament-like structure at level Ⅰ was the uterosacral ligament, which acted as the main apical fixation in the sacral direction, while the cardinal ligament was mainly composed of vascular system, lymph-vessels and loose connective tissue around them, lacking the tough connective tissue structures, which was connected to the internal iliac vascular system. There were no strong ligaments connected to the tendinous arch of pelvic fascia (ATFP) at the lateral side of vaginal wall at level Ⅱ. ATFP was the edge of the superior fascia of pelvic diaphragm, which was bounded by the fascia of the obturator. Its surface was smooth and close to the levator ani muscle and fuses with the vaginal fascia in about one thirds of middle lower segments of the vagina. When the ureter tunnel is separated, dense connective structures can be found in both anterior and posterior walls near the intersection of the ureter across uterine artery, fixing the bilateral angle of the bladder triangle, starting from the cervix and vagina and ending in the tunica muscularis vesicae urinariae. CONCLUSIONS: Based on the laparoscopic anatomy, the pelvic floor fascia ligament support above the levator ani muscle can be considered mainly around the vagina, and fascial ligament above the levator ani muscle can be simply considered as two parallel planes forming a "double hammock" structure, which may provide more anatomic data for pelvic floor reconstruction.


Assuntos
Laparoscopia , Ligamentos , Vagina , Feminino , Humanos , Ligamentos/anatomia & histologia , Diafragma da Pelve , Bexiga Urinária , Vagina/anatomia & histologia , Vagina/cirurgia
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