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1.
BMC Genomics ; 21(1): 139, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32041524

RESUMO

BACKGROUND: Lead (Pb) pollution in soil has become one of the major environmental threats to plant growth and human health. Safe utilization of Pb contaminated soil by phytoremediation require Pb-tolerant rapeseed (Brassica napus L.) accessions. However, breeding of new B. napus cultivars tolerance to Pb stress has been restricted by limited knowledge on molecular mechanisms involved in Pb tolerance. This work was carried out to identify genetic loci related to Pb tolerance during seedling establishment in rapeseed. RESULTS: Pb tolerance, which was assessed by quantifying radicle length (RL) under 0 or 100 mg/L Pb stress condition, shown an extensive variation in 472 worldwide-collected rapeseed accessions. Based on the criterion of relative RL > 80%, six Pb-tolerant genotypes were selected. Four quantitative trait loci (QTLs) associated with Pb tolerance were identified by Genome-wide association study. The expression level of nine promising candidate genes, including GSTUs, BCATs, UBP13, TBR and HIPP01, located in these four QTL regions, were significantly higher or induced by Pb in Pb-tolerant accessions in comparison to Pb-sensitive accessions. CONCLUSION: To our knowledge, this is the first study on Pb-tolerant germplasms and genomic loci in B. napus. The findings can provide valuable genetic resources for the breeding of Pb-tolerant B. napus cultivars and understanding of Pb tolerance mechanism in Brassica species.

2.
Diabetes Metab Syndr Obes ; 13: 405-412, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32110073

RESUMO

Objective: To analyse the clinical features of pneumomediastinum associated with DKA (diabetic ketoacidosis) to improve clinicians' understanding of the disease. Methods: A total of 78 patients with pneumomediastinum associated with DKA were identified in the literature search, and one patient treated in our hospital was included. The clinical features of the 79 patients were retrospectively analysed, and the pathogenesis, clinical symptoms, diagnostic methods, treatment strategies and prognoses were explored. Results: All cases were confirmed by chest CT (computed tomography), and Kussmaul respiration, severe vomiting, chest pain were common symptoms. The main treatment principle was to correct acidosis and treat the primary disease. The majority of patients recovered within 1-2 weeks, and only 2 patients died. Conclusion: Pneumomediastinum associated with DKA is a rare disease, and it has a benign course when an early diagnosis is made and aetiological treatment is administered.

3.
Luminescence ; 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31999894

RESUMO

A dual-function fluorescent probe (Probe 1) was developed for discriminative detection of Hg2+ and N2 H4 . Probe 1 could discriminatively detect Hg2+ and N2 H4 through two different reaction sites, with the mechanism for Probe 1 for Hg2+ depending on a desulfurization reaction and for N2 H4 depending on the Schiff-base reaction. N2 H4 had minimal effect on Hg2+ detection in dimethyl sulfoxide (DMSO)/H2 O solution, but Hg2+ could interfere with N2 H4 detection in DMSO/buffer solution. Different concentrations of Hg2+ and N2 H4 resulted in different blue shades of Probe 1 test strips, and the shade of blue was different with the same concentration of Hg2+ or N2 H4 , as observed under ultraviolet light at 365 nm wavelength.

5.
Res Vet Sci ; 128: 162-169, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31809973

RESUMO

Bovine viral diarrhea virus type 1 (BVDV-1) is prevalent worldwide and causes significant economic loss in animal husbandry. Since its first report in the 1980s in China, several genotypes of BVDV-1 had been reported, but an in-depth phylogenetic analysis on the BVDV isolates from China is lacking. To investigate the molecular evolution and phylodynamics of BVDV-1 genotypes circulating in China, comprehensive phylogenetic and phylodynamic analyses were performed to reconstruct the origin and spatial-temporal distribution, and to trace main viral flows among different areas. BVDV-1 5'-UTR sequences from China and Mongolia were collected from Genbank, and the phylogeny was built using the maximum likelihood method. The Bayesian Skygrid was used to estimate the evolution and population dynamics of BVDV-1. Eight BVDV-1 genotypes were identified, of which 1b and 1 m are the main genotypes. The results indicated that BVDV-1 might be introduced in China in the 1960s, and after a long period of population growth, it gradually leveled off after 2010. The phylodynamic inference clearly shows a more steady BVDV-1 population growth, and the transmission of BVDV-1 may be confined to specific regions. This study will help to understand the molecular epidemiology and long-term evolutionary dynamics of BVDV-1 in China, therefore providing a scientific basis for the prevention and controlof the virus.

6.
Neural Netw ; 121: 474-483, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31630087

RESUMO

In this paper, the leader-following consensus problem of a class of nonlinearly multi-dimensional multi-agent systems with actuator faults is addressed by developing a novel neural network learning strategy. In order to achieve the desirable consensus results, a neural network learning algorithm composed of adaptive technique is proposed to on-line approximate the unknown nonlinear functions and estimate the unknown bounds of actuator faults. Then, on the basis of the approximations and estimations, a robust adaptive distributed fault-tolerant consensus control scheme is investigated so that the bounded results of all signals of the resulting closed-loop leader-following system can be achieved by using Lyapunov stability theorem. Finally, efficiency of the proposed adaptive neural network learning strategy-based consensus control strategies is demonstrated by a coupled nonlinear forced pendulums system.


Assuntos
Aprendizado de Máquina , Consenso , Dinâmica não Linear
7.
Neuropsychopharmacology ; 45(2): 358-366, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31634898

RESUMO

Predicting the probability of converting from mild cognitive impairment (MCI) to Alzheimer's disease (AD) is still a challenging task. This study aims at providing a personalized MCI-to-AD conversion estimation by using a multipredictor nomogram that integrates neuroimaging features, cerebrospinal fluid (CSF) biomarker, and clinical assessments. To do so, 290 MCI patients were collected from the Alzheimer's Disease Neuroimaging Initiative (ADNI), of whom 76 has converted to AD and 214 remained with MCI. All subjects were randomly divided into a primary and validation cohort. Radiomics signature (Rad-sig) was obtained based on 17 cerebral cortex features selected by using Least Absolute Shrinkage and Selection Operator (LASSO) algorithm. Clinical factors and amyloid-beta peptide (Aß) concentration were selected by using Spearman correlation between the converted and not-converted patients. Then, a nomogram that combines image features, clinical factor, and Aß concentration was constructed and validated. Furthermore, we explored the associations between various predictors from the macro- to the microperspective by assessing gene expression patterns. Our results showed that the multipredictor nomogram (C-index 0.978 and 0.956 in both cohorts, respectively) outperformed the nomogram using either Rad-sig or Aß concentration as individual predictors. Significant associations were found between neuropsychological scores, cerebral cortex features, Aß levels, and underlying gene pathways. Our study may have a clinical impact as a powerful predictive tool for predicting the conversion probability of MCI and providing associations between cognitive impairment, structural changes, Aß levels, and underlying biological patterns from the macro- to the microperspective.

8.
Int Immunopharmacol ; 79: 106108, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31881376

RESUMO

Diabetic nephropathy (DN) is the most paradigmatic complication of diabetes mellitus (DM) and brings about severe social and economic burdens. BML-111 is a potent agonist of Lipoxin A4 and has shown anti-inflammatory function in many diseases. The aim of the study is to investigate the effects of BML-111 on high glucose (HG) -induced mesangial cells. HBZY-1 cells were stimulated by HG with or without BML-111. ML385 was used as an Nrf2 inhibitor. Cell proliferation was measured by CC-K 8 assay. Besides, levels of TNF-α, IL-1, IL-6 and MCP-1 were detected by corresponding ELISA kits. DCFH-DA staining and an available ROS kit were employed to determine the ROS generation. In addition, extracellular matrix (ECM) accumulation was evaluated by immunofluorescence assay and western blot analysis. The protein expressions involved in Nrf2/HO-1 and MAPK pathway were assessed by western blot assay. Results indicated that BML-111 extremely inhibited HBZY-1 cell proliferation induced by HG. Moreover, BML-111 reduced the levels of TNF-α, IL-1, IL-6 and MCP-1, declined intracellular ROS level, and attenuated expression of ECM proteins laminin, fibronectin, collagen IV and TGF-ß1. In addition, BML-111 promoted the activation of Nrf2, HO-1, and NQO1, while suppressed the phosphorylation of p38 and JNK. Further, NRF2 silence reversed the inhibitory effects of BML-111 on HG-induce inflammation, oxidative stress and ECM accumulation, accelerate the MAPK signaling, and diminished the expression of Nrf2 pathway. In summary, BML-111 alleviated HG-induced injury in HBZY-1 cells by repressing inflammatory response, oxidative stress and ECM accumulation via activating Nrf2 and inhibiting MAPK pathway.

11.
J Biosci ; 44(4)2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31502558

RESUMO

Accumulating evidence suggest that microRNAs play crucial roles in the development and progression of bladder cancer (BC). Here, we found that miR-212-3p was significantly down-regulated and negatively correlated with nuclear factor IA (NFIA) in human BC tissues. Bioinformatics analysis predicted that NFIA was a target gene of miR-212-3p. Then BC cell lines, T24 and J82 cells were transfected with miR-212-3p mimics or siNFIA to obtain miR-212-3p overexpression or NFIA knockdown cell lines, respectively. Quantitative real-time PCR was used to determine the expression of miR-212-3p and NFIA. Western blot analysis was utilized to detect NFIA expression. MTT assay showed either miR-212-3 overexpression or NFIA knockdown significantly inhibited the BC cell proliferation. Double staining with Annexin V-APC and 7-AAD showed the total number of apoptotic BC cells were remarkably increased after miR-212-3p overexpression or NFIA knockdown. Collectively, our results indicated that miR-212-3p targeting NFIA might serve as a promising target for BC.


Assuntos
Proliferação de Células/genética , MicroRNAs/genética , Fatores de Transcrição NFI/genética , Neoplasias da Bexiga Urinária/genética , Idoso , Apoptose/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias da Bexiga Urinária/patologia
12.
Int J Biol Sci ; 15(9): 2006-2015, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31523200

RESUMO

The p53 is one of the most important tumor suppressors through surveillance of DNA damages and abnormal proliferation signals, and activation the cell cycle arrest and apoptosis in response to stress. However, the mutation of p53 is known to be oncogenic by both loss of function in inhibiting cell cycle progress and gain of function in promoting abnormal proliferation. In the present study, we have established a knock in mouse model containing an Asn-to-Ser substitution at p53 amino acid 236 by homologous recombination (p53N236S). Other than tumorigenesis phenotype, we found that p53S/S mice displayed female-specific phenotype of open neural tube in brain (exencephaly) and spinal cord (spina bifida). The occurrence rate for embryonic exencephaly is 68.5% in female p53S/S mice, which is much more than that of in p53-/- mice (37.1%) in the same genetic background. Further study found that p53N236S mutation increased neuronal proliferation and decreased neuronal differentiation and apoptosis. To rescue the phenotype, we inhibited cell proliferation by crossing Wrn-/- mice with p53S/S mice. The occurrence of NTDs in p53S/S Wrn-/- mice was 35.2%, thus suggesting that the inhibition of cell proliferation through a Wrn defect partially rescued the exencephaly phenotype in p53S/S mice. We also report that p53S decreased expression of UTX at mRNA and protein level via increasing Xist transcript, result in high female-specific H3K27me3 expression and repressed Mash1 transcription, which facilitating abnormal proliferation, differentiation, and apoptosis, result in the mis-regulation of neurodevelopment and neural tube defects (NTDs).

13.
Nat Plants ; 5(8): 822-832, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31383969

RESUMO

The non-random three-dimensional (3D) organization of the genome in the nucleus is critical to gene regulation and genome function. Using high-throughput chromatin conformation capture, we generated chromatin interaction maps for Brassica rapa and Brassica oleracea at a high resolution and characterized the conservation and divergence of chromatin organization in these two species. Large-scale chromatin structures, including A/B compartments and topologically associating domains, are notably conserved between B. rapa and B. oleracea, yet their KNOT structures are highly divergent. We found that genes retained in less fractionated subgenomes exhibited stronger interaction strengths, and diploidization-resistant duplicates retained in pairs or triplets are more likely to be colocalized in both B. rapa and B. oleracea. These observations suggest that spatial constraint in duplicated genes is correlated to their biased retention in the diploidization process. In addition, we found strong similarities in the epigenetic modification and Gene Ontology terms of colocalized paralogues, which were largely conserved across B. rapa and B. oleracea, indicating functional constraints on their 3D positioning in the nucleus. This study presents an investigation of the spatial organization of genomes in Brassica and provides insights on the role of 3D organization in the genome evolution of this genus.

14.
ACS Omega ; 4(3): 4918-4926, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-31459676

RESUMO

Two sensitive and ratiometric fluorescent probes (probe I and probe II) were developed for the detection of fluoride ions. Probe I can detect fluoride ions quantitatively within a range of 0-6 µM and a detection limit of 73 nM, while probe II has a range of 0-40 µM and a detection limit of 138 nM. The test strips from probe I are quickly able to recognize F- (5 min) inside of the F- safety level in drinking water (1.0 mg/L, ∼5 µM) under 254 nm ultraviolet light, and the test strips from probe II quickly recognize F- (12 min) in dangerously high F- levels in water (4.0 mg/L, ∼21 µM) under 254 nm ultraviolet light. This combination of fluorescent paper sensors from probe I and probe II can be used as a simple and convenient tool to determine whether water is safe to drink or dangerous.

15.
Food Sci Biotechnol ; 28(4): 983-990, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31275698

RESUMO

A new fluorescent probe made from (E)-2-(benzo[d]thiazol-2-yl)-3-(6-hydroxynaphthalen-2-yl) acrylonitrile (Probe 1) was synthesized for the determination of bisulfite concentrations in real food samples (red wine and sugar). Adding bisulfite to a Probe 1 solution caused a marked decrease in fluorescence intensity and a visual color change from yellow to light yellow. This distinct color response indicates that Probe 1 could be used as a visual sensor for bisulfite. Probe 1 can detect bisulfite quantitatively in the range 0-400 µM with a detection limit of 0.10 µM. This makes Probe 1 a convenient signaling instrument for determining bisulfite levels in sugar and red wine samples.

16.
Mol Genet Genomic Med ; 7(8): e778, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31228357

RESUMO

BACKGROUND: MIR155HG plays an important role in malignant tumors, but it is rarely reported in the occurrence and development of colorectal cancer (CRC). This study investigated the effects of MIR155HG polymorphisms on CRC susceptibility from the perspective of molecular genetics. METHODS: Eight SNPs in MIR155HG were selected and genotyped among 514 CRC cases and 510 healthy controls using the Agena MassARRAY platform. The associations between these SNPs and the CRC risk were evaluated under genetic models using conditional logistic regression analysis. The HaploReg v4.1 database was used for SNPs functional prediction. RESULTS: The allele "C" of rs12482371 (p = 0.047), allele "C" of rs1893650 (p = 0.025), and the allele "A" of rs928883 (p = 0.037) in MIR155HG were significantly associated with CRC risk. Genetic model analysis revealed that rs12482371 and rs1893650 increased CRC risk; whereas rs928883 was associated with reduced CRC risk. Stratification analysis showed that rs9383938 was a protective factor in CRC patients under 60 years old. Rs12482371 and rs1893650 were associated with the CRC risk in females. Rs11911469 and rs34904192 may affect the clinical stage and lymph node metastasis. Moreover, the haplotypes CTT and GTC of LD block rs4143370|rs77218221|rs12482371, and the haplotypes CATGA and CACGG of LD block rs77699734|rs11911469|rs1893650|rs34904192|rs928883 were significantly associated with CRC risk. CONCLUSION: This study revealed that MIR155HG SNPs were associated with CRC susceptibility and could be predictive biomarkers for CRC risk.

17.
Biomed Res Int ; 2019: 1603712, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31223611

RESUMO

Objectives: To build a guideline for the individual treatment of Tetralogy of Fallot (TOF) with major aortopulmonary collaterals (MAPCAs) and tentatively establish the occlusion index of MAPCAs. Methods: According to the diameter of the aortopulmonary collaterals (R: mm) and the bodyweight of the children (weight: kg), K= ((∑R 2)/Wt) was set as the occlusion index of TOF with MAPCAs. A retrospective study was initially performed in 171 patients who suffered from TOF with MAPCAs and underwent cardiac malformation repair to investigate the intervals of the K value: K≥2, 1

Assuntos
Tetralogia de Fallot/diagnóstico por imagem , Tetralogia de Fallot/fisiopatologia , Tetralogia de Fallot/cirurgia , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Artéria Pulmonar/diagnóstico por imagem , Artéria Pulmonar/fisiopatologia , Artéria Pulmonar/cirurgia , Estudos Retrospectivos
18.
Arch Virol ; 164(8): 2119-2129, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31147766

RESUMO

Rabies is a fatal disease caused by infection with rabies virus (RABV), and human rabies is still a critical public-health concern in China. Although there have been some phylogenetic studies about RABV transmission patterns, with the accumulation of more rabies sequences in recent years, there is an urgent need to update and clarify the spatial and temporal patterns of RABV circulating in China on a national scale. In this study, we collected all available RABV nucleoprotein gene sequences from China and its neighboring countries and performed comparative analysis. We identified six significant subclades of RABV circulating in China and found that each of them has a specific geographical distribution, reflecting possible physical barriers to gene flow. The phylogeographic analysis revealed minimal viral movement among different geographical locations. An analysis using Bayesian coalescent methods indicated that the current RABV strains in China may come from a common ancestor about 400 years ago, and currently, China is amid the second event of increasing RABV population since the 1950s, but the population has decreased gradually. We did not detect any evidence of recombination in the sequence dataset, nor did we find any evidence for positive selection during the expansion of RABV. Overall, geographic location and neutral genetic drift may be the main factors in shaping the phylogeography of RABV transmission in China.


Assuntos
Vírus da Raiva/genética , Raiva/transmissão , Animais , Teorema de Bayes , China , Evolução Molecular , Humanos , Epidemiologia Molecular/métodos , Nucleoproteínas/genética , Filogenia , Filogeografia/métodos , RNA Viral/genética , Raiva/virologia , Análise de Sequência de DNA/métodos
19.
Cancer Biol Med ; 16(1): 79-102, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31119048

RESUMO

Objective: Polycystic kidney disease (PKD) is the major cause of kidney failure and mortality in humans. It has always been suspected that the development of cystic kidney disease shares features with tumorigenesis, although the evidence is unclear. Methods: We crossed p53 mutant mice (p53N236S, p53S) with Werner syndrome mice and analyzed the pathological phenotypes. The RNA-seq, ssGSEA analysis, and real-time PCR were performed to dissect the gene signatures involved in the development of disease phenotypes. Results: We found enlarged kidneys with fluid-filled cysts in offspring mice with a genotype of G3mTerc -/- WRN -/- p53 S/S (G3TM). Pathology analysis confirmed the occurrence of PKD, and it was highly correlated with the incidence of tumorigenesis. RNA-seq data revealed the gene signatures involved in PKD development, and demonstrated that PKD and tumorigenesis shared common pathways, including complement pathways, lipid metabolism, mitochondria energy homeostasis and others. Interestingly, this G3TM PKD and the classical PKD1/2 deficient PKD shared common pathways, possibly because the mutant p53S could regulate the expression levels of PKD1/2, Pkhd1, and Hnf1b. Conclusions: We established a dual mouse model for PKD and tumorigenesis derived from abnormal cellular proliferation and telomere dysfunction. The innovative point of our study is to report PKD occurring in conjunction with tumorigenesis. The gene signatures revealed might shed new light on the pathogenesis of PKD, and provide new molecular biomarkers for clinical diagnosis and prognosis.

20.
BMC Genomics ; 20(1): 317, 2019 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-31023229

RESUMO

BACKGROUND: WUSCHEL-related homeobox (WOX) genes encoding plant-specific homeobox (HB) transcription factors play important roles in the growth and development of plants. To date, WOX genes has been identified and analyzed in many polyploids (such as cotton and tobacco), but the evolutionary analysis of them during polyploidization is rare. With the completion of genome sequencing, allotetraploid Brassica napus and its diploid progenitors (B. rapa and B. oleracea) are a good system for studying this question. RESULTS: In this study, 52, 25 and 29 WOX genes were identified in allotetraploid B. napus (2n = 4x = 38, AnCn), the An genome donor B. rapa (2n = 2x = 20, Ar) and the Cn genome donor B. oleracea (2n = 2x = 18, Co), respectively. All identified WOX genes in B. napus and its diploid progenitors were divided into three clades, and these genes were selected to perform gene structure and chromosome location analysis. The results showed that at least 70 and 67% of WOX genes maintained the same gene structure and relative position on chromosomes, respectively, indicating that WOX genes in B. napus were highly conserved at the DNA level during polyploidization. In addition, the analysis of duplicated genes and transposable elements (TEs) near WOX genes showed that whole-genome triplication (WGT) events, segmental duplication and abundant TEs played important roles in the expansion of the WOX gene family in B. napus. Moreover, the analysis of the expression profiles of WOX gene pairs with evolutionary relationships suggested that the WOX gene family may have changed at the transcriptional regulation level during polyploidization. CONCLUSIONS: The results of this study increased our understanding of the WOX genes in B. napus and its diploid progenitors, providing a rich resource for further study of WOX genes in these species. In addition, the changes in WOX genes during the process of polyploidization were discussed from the aspects of gene number, gene structure, gene relative location and gene expression, which provides a reference for future polyploidization analysis.


Assuntos
Brassica napus/genética , Genoma de Planta , Proteínas de Homeodomínio/genética , Mapeamento Cromossômico , Elementos de DNA Transponíveis/genética , Diploide , Regulação da Expressão Gênica de Plantas , Proteínas de Homeodomínio/classificação , Família Multigênica , Filogenia , Poliploidia
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