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1.
Dalton Trans ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32129387

RESUMO

A series of host-guest materials containing polyoxometalate anions and lanthanide-organic layers have been synthesized and structurally characterized. By anion-π interactions between the anions and the π-acidic naphthalenediimide moieties, the materials emit strong red room-temperature phosphorescence and exhibit reversible photochromism.

2.
BMC Genomics ; 21(1): 112, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32005168

RESUMO

BACKGROUND: In sorghum (Sorghum bicolor), one paramount breeding objective is to increase grain quality. The nutritional quality and end use value of sorghum grains are primarily influenced by the proportions of tannins, starch and proteins, but the genetic basis of these grain quality traits remains largely unknown. This study aimed to dissect the natural variation of sorghum grain quality traits and identify the underpinning genetic loci by genome-wide association study. RESULTS: Levels of starch, tannins and 17 amino acids were quantified in 196 diverse sorghum inbred lines, and 44 traits based on known metabolic pathways and biochemical interactions amongst the 17 amino acids calculated. A Genome-wide association study (GWAS) with 3,512,517 SNPs from re-sequencing data identified 14, 15 and 711 significant SNPs which represented 14, 14, 492 genetic loci associated with levels of tannins, starch and amino acids in sorghum grains, respectively. Amongst these significant SNPs, two SNPs were associated with tannin content on chromosome 4 and colocalized with three previously identified loci for Tannin1, and orthologs of Zm1 and TT16 genes. One SNP associated with starch content colocalized with sucrose phosphate synthase gene. Furthermore, homologues of opaque1 and opaque2 genes associated with amino acid content were identified. Using the KEGG pathway database, six and three candidate genes of tannins and starch were mapped into 12 and 3 metabolism pathways, respectively. Thirty-four candidate genes were mapped into 16 biosynthetic and catabolic pathways of amino acids. We finally reconstructed the biosynthetic pathways for aspartate and branched-chain amino acids based on 15 candidate genes identified in this study. CONCLUSION: Promising candidate genes associated with grain quality traits have been identified in the present study. Some of them colocalized with previously identified genetic regions, but novel candidate genes involved in various metabolic pathways which influence grain quality traits have been dissected. Our study acts as an entry point for further validation studies to elucidate the complex mechanisms controlling grain quality traits such as tannins, starch and amino acids in sorghum.

3.
Chem Commun (Camb) ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32003370

RESUMO

Anionic POMs prefer to bond with positive metal cations instead of neutral or negative organic ligands. Therefore, it is challenging to synthesize POM-based MOFs, let alone bi-POM-based host-guest MOFs. In this work, an unprecedented bi-POM-based host-guest MOF, Na[Ni(enMe)2]4[Ni(enMe)2(H2O)2]2{[Ni6(µ3-OH)3(enMe)3 (SIP)1.5(B-α-PW9O34)]2[H3PNiW11O40]}·5enMe·33H2O (1), with Ni6-capped [PW9O34] as the node of the host framework and Keggin-type [PNiW11O40] units as the guest was synthesized. 1 showed excellent chemical stability towards aqueous solutions of pH 2-12 at both ambient and boiling temperature, providing opportunities for its application in fresh water harvesting from air.

4.
Epigenomics ; 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32079426

RESUMO

Aim: We aimed to explore the circular RNA (circRNA) profile of small-cell lung cancer (SCLC). Materials & methods: Total RNA was extracted from six paired SCLC tumors and adjacent noncancerous tissues. Next-generation sequencing was performed to identify the circRNA expression profile of SCLC. Results: We found that five circRNAs were significantly upregulated and 30 circRNAs were significantly downregulated in the SCLC tissues. We confirmed the five upregulated and four randomly selected downregulated circRNAs using real-time quantitative PCR. Notably, circ-STXBP5L was selectively upregulated in SCLC samples, but undetectable in the normal control tissues. Bioinformatics analysis demonstrated that circ-STXBP5L may participate in SCLC carcinogenesis by regulating numerous cancer-related pathways. Conclusion: This study may provide new insights into the early diagnosis and development of targeted therapies for SCLC.

5.
Small ; 15(45): e1903270, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31535783

RESUMO

Metal-free ultralong organic phosphorescence (UOP) materials have attracted significant attention owing to their anomalous photophysical properties and potential applications in various fields. Here, three pyrimidine-based organic luminogens, 9-(pyrimidin-2-yl)-9H-carbazole, 9-(4,6-dimethylpyrimidin-2-yl)-9H-carbazole, and 9-(5-bromopyrimidin-2-yl)-9H-carbazole are designed and synthesized, which show efficient yellow UOP with the longest lifetimes up to 1.37 s and the highest absolute phosphorescence quantum yields up to 23.6% under ambient conditions. Theoretical calculations, crystal structures, and photophysical properties of these compounds reveal that intramolecular hydrogen bonding, intermolecular π-π interactions, and intermolecular electronic coupling are responsible for forming dimers and generating highly efficient UOP. Their efficacy as solid materials for data encryption is demonstrated.

6.
Oncotarget ; 10(40): 4069-4078, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31258850

RESUMO

Neoadjuvant therapy improves long-term locoregional control and overall survival after surgical resection for esophageal cancer, and neoadjuvant chemotherapy (nCT) or neoadjuvant chemoradiotherapy (nCRT) are commonly used in clinical practice. Nimotuzumab is a humanized monoclonal antibody against epidermal growth factor receptor (EGFR), the efficacy of nimotuzumab added to nCRT for esophageal cancer is uncertain. We conducted this retrospective study in which combining neoadjuvant treatment of nimotuzumab with chemoradiotherapy (Nimo-nCRT) is compared with nCRT and nCT for patients with potentially resectable locally advanced esophageal squamous cell carcinoma. One hundred ninety-five patients received neoadjuvant therapy and 172 (88.2%) underwent esophagectomy. Surgical resection was performed in 94.4% after Nimo-nCRT, versus 92.5% after nCRT and 83.5% after nCT (P = 0.026). The R0 resection rate was 100% after Nimo-nCRT, 95.9% after nCRT and 92.6% after nCT (P = 0.030). Pathological complete response (pCR) was achieved in 41.2% after Nimo-nCRT, versus 32.4% after nCRT and 14.8% after nCT (P = 0.0001). Lymph-node metastases were observed in 29.4% in the Nimo-nCRT group, versus 21.6% in the nCRT group and 35.8% in the nCT group (P = 0.093). More patients in the Nimo-nCRT and nCRT group developed grade 3 esophagitis compared to those in the nCT group, P = 0.008. There was no difference in surgical complications between the treatment groups. nCRT results in improved R0 resection, higher pCR rate, and a lower frequency of lymph node metastases compared to nCT, adding nimotuzumab to nCRT is safe and appears to facilitate complete resection and increase the pCR rate.

7.
J Xray Sci Technol ; 27(3): 559-572, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31177257

RESUMO

BACKGROUND: For sparse and limited angle projection Computed Tomography (CT), the reconstructed image usually suffers from considerable artifacts due to undersampled data. OBJECTIVE: To improve image reconstruction quality of sparse and limited angle projection CT, this study tested a novel reconstruction algorithm based on Dictionary Learning (DL) from sparse and limited projections. METHODS: The study used signal sparse representation and feature extraction to render the DL technology, which is constrained by L2 and Lp norms, respectively. A Lp Norm Dictionary Learning term is suitable for regular term of objective function for CT image reconstruction. This is helpful for solving the objective function by combining algorithm of ART. Based on these features, the new algorithm of ART-DL-Lp is proposed for CT image reconstruction. The alternate solving strategy of the algorithm of "ART first, then adaptive DL" is provided in sequence. The impact on reconstruction results of ART-DL-Lp at different p values (0 < p < 1) is also considered. RESULTS: For non-ideal projections with noise, the digital experiments show that ART-DL-Lp data were superior to those of ART, SART, and ART-DL-L2. Accordingly, the objective evaluation metrics for non-ideal situation of RMSE, MAE, PSNR, Residuals and SSIM are all better than those of contrasted three algorithms. The metrics curves of ART-DL-Lp algorithm are recorded as the best. In both incomplete projection situations, smaller p-value of ART-DL-Lp algorithm induces more close reconstructed images to the original form and better five objective evaluation metrics. CONCLUSIONS: Overall, the reconstruction efficiency of the proposed ART-DL-Lp for CT imaging using the noisy incomplete projections outperforms ART, SART and ART-DL-L2 algorithms. For ART-DL-Lp algorithm, lower p-values result in better reconstruction performance.

8.
Dalton Trans ; 48(27): 9930-9934, 2019 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-31197290

RESUMO

A Ho-MOF ([Ho(SIP)(H2O)5]·3H2O (1), NaH2SIP = monosodium 5-sulfoisophthalic acid) was designed and synthesized for proton conductivity. It showed a proton conductivity of 8.2 × 10-4 S cm-1 at 343 K and 98% relative humidity. Besides, compound 1 showed excellent water and chemical stability as well as reversible dehydration and hydration closely related to the proton conductivity.

9.
Mol Genet Genomic Med ; 7(7): e00741, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31144478

RESUMO

BACKGROUND: Alport Syndrome (AS) is a progressive hereditary glomerular disease. It is often accompanied by sensorineural hearing loss and ocular abnormalities and can sometimes develop into end stage renal disease (ESRD), which is caused by mutations in the genes encoding the collagen type IV family of proteins. METHODS: This study analyzed the association between the clinical data of seven AS families and genes and the disease progression of different mutation types, including COL4A3 (OMIM 120070),COL4A4 (OMIM 120131), and COL4A5 (OMIM303630). RESULTS: A total of six new pathogenic mutation sites, one complex heterozygous mutation at COL4A3, and a combined mutation of COL4A5 and INF2 (OMIM 610982) were identified in this study. It was revealed that the clinical manifestations of X-linked AS caused by mutations in the COL4A5 gene were more severe in males than in females. In addition, the difference in patient phenotype can be attributed to the location of gene mutations affecting the protein domain or functional domain. Our data suggested that the gene deletion and nonsense mutations had a high risk for progression to ESRD. CONCLUSION: Our results revealed the spectrum of type IV collagen genes, which contribute to the enrichment of database resources and has important implications in the diagnosis, prognosis, and guiding treatment of AS.

10.
Des Monomers Polym ; 22(1): 66-78, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30863244

RESUMO

N-phenylmaleimide (N-PMI) and its precursor, (Z)-4-oxo-4-(phenylamino)but-2-enoic acid, were synthesized from aniline and maleic anhydride. Copolymerization between N-phenylmaleimide and styrene was initiated in micropores and outside surface of porous polyvinyl chloride (PVC) resin suspended in aqueous phase. The modified PVC was characterized with Gel Permeation Chromatography, thermal gravimetric analysis and scanning electron microscopy. The result of high performance liquid chromatography shows that the purity of N-PMI reached 97.3%. Thermal gravimetric analysis indicated that the introduction of N-PMI could clearly affect the thermal degradation behavior of PVC, and when PMI was at 6.25% of the PVC mass, the decomposition temperature T0.5 of modified polymer was increased to 314.2°C. The glass transition temperature of modified polymer was increased from 70°C to 80°C.

11.
Cell Death Discov ; 5: 76, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30886744

RESUMO

Polycystic kidney disease (PKD) is a common hereditary kidney disease with abnormal proliferation and apoptosis of kidney cystic epithelial cells, eventually leading to chronic renal failure. Currently, there are no effective treatment methods. Similar to tumor cells, cystic epithelial cells have abnormal glycolysis and over-activation of proliferation signaling pathways. In the present study, for the first time, we investigated the effects of low-dose combinational use of 2-deoxyglucose (2-DG) and metformin (MET) on the proliferation and apoptosis in the human cystic kidney epithelial cells. Cystic epithelia cells were divided into control group, 2-DG group, MET group and 2-DG+MET group. Cell Proliferation, apoptosis and glucose metabolism were measured in each group. The results showed that low-dose combinational treatment of 2-DG and MET significantly inhibited the proliferation of renal cystic epithelial cells by suppressing the activities of PKA, mTOR and ERK signaling pathways and upregulating PI3K/Akt pathway. Combination of both drugs increased the apoptosis rates of cystic epithelial cells. Two drugs inhibited glucose metabolic phenotypes, glycolysis and oxidative phosphorylation, and significantly lowered the intracellular ATP level in cystic epithelial cells. 2-DG could also neutralize excessive production of lactate (lactic acidosis) caused by MET and both drugs had complementary effect for cystic epithelial cells. These results reveal that combinational use of low-dose 2-DG and MET can markedly inhibit proliferation via modulating glucose metabolic phenotypes in human polycystic kidney epithelial cells, low-dose combinational use of both drugs can also lower the toxic effects of each drug, and is a novel strategy for future treatment of human polycystic kidney disease.

12.
Chem Commun (Camb) ; 55(11): 1643-1646, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30657485

RESUMO

A sextuple ordered interpenetrated copper-naphthalenediimide network has been constructed by combining the features of porous metal-organic frameworks and π-conjugated supramolecular aggregation. The material exhibits intrinsic semiconductive features with narrow bandgap energy (1.2 eV) and outstanding electron transport. Theoretical calculations combined with experiments indicate that the high electron conduction may originate from π-d coupling and J-aggregation.

13.
Int Urol Nephrol ; 51(2): 207-213, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30536191

RESUMO

PURPOSE: The purpose of the study was to evaluate the efficacy and safety of a patented perfusion and suctioning platform and ureteral access sheath in the treatment of large ureteral stones (≥ 1.5 cm) below L4 level. METHODS: We recruited 122 patients with large ureteral stones below L4 level at our hospital from December 2014 to June 2017. The patients were randomly divided into the study and control groups. Multiple operative and perioperative parameters were compared between the two groups. RESULTS: The study group had shorter operation time, less cases of postoperative fever, lower serum levels of PCT, IL-6 and BET within 24 h after surgery, as well as less number of cases receiving secondary surgery than the control group. Moreover, the former had a significantly higher stone clearance rate than the latter (P < 0.05; t-test or χ2 test). CONCLUSIONS: The patented perfusion and suctioning platform and ureteral access sheath are safe and effective in treating large ureteral stones (≥ 1.5 cm) below L4 level.


Assuntos
Febre , Perfusão , Complicações Pós-Operatórias/diagnóstico , Sucção , Ureter , Ureteroscopia , Infecções Urinárias , Procedimentos Cirúrgicos Urológicos , Adulto , Feminino , Febre/diagnóstico , Febre/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Perfusão/efeitos adversos , Perfusão/instrumentação , Perfusão/métodos , Sucção/efeitos adversos , Sucção/instrumentação , Sucção/métodos , Resultado do Tratamento , Ureter/diagnóstico por imagem , Ureter/cirurgia , Cálculos Ureterais/diagnóstico , Cálculos Ureterais/fisiopatologia , Cálculos Ureterais/cirurgia , Ureteroscopia/efeitos adversos , Ureteroscopia/métodos , Infecções Urinárias/diagnóstico , Infecções Urinárias/etiologia , Procedimentos Cirúrgicos Urológicos/efeitos adversos , Procedimentos Cirúrgicos Urológicos/instrumentação , Procedimentos Cirúrgicos Urológicos/métodos
14.
Infect Genet Evol ; 72: 147-150, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30447296

RESUMO

Mutations in pncA gene contributing to PZA resistance was not clearly elucidated in China. To reveal the correlated mutations of pncA gene on pyrazinamide (PZA) resistance. 148 Mycobacterium tuberculosis clinical isolates were included from multi-drug resistant tuberculosis suspects. The MGIT 960 test and microscopic observation drug susceptibility (MODS) assay were adopted for PZA phenotype drug susceptibility test. 120 isolates with consistent MGIT 960 and MODS results were selected for pncA gene sequencing. 68 samples (56.7%) were resistant to PZA while leaving 52 PZA susceptible samples. Out of the 68 PZA resistant isolates, 49 (72.1%) harbored mutations of pncA, and 4 (7.7%) of the 52 PZA susceptible samples harbored mutations of pncA as well. Compared to the phenotype drug resistant pattern of PZA, the mutations of pncA gene reached a sensitivity of 0.72 to report PZA resistance and a specificity of 0.92 to predict PZA susceptibility. Those mutations, Gln10Pro, Asp12Ala, Tyr41Stop, Gly97Asp, Val128Gly and FSC131(ins) exceeding 5% of the total PZA resistant isolates of each, might be helpful but not adequate in PZA molecular susceptibility test design and development.

15.
Br J Pharmacol ; 176(5): 711-724, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30515768

RESUMO

BACKGROUND AND PURPOSE: The pathogenic mechanism of autosomal dominant polycystic kidney disease (ADPKD) is unclear. Similar to tumour cells, polycystic kidney cells are primarily dependent on aerobic glycolysis for ATP production. Compared with rodents, miniature pigs are more similar to humans. This study is the first time to investigate the effects of the combination of metformin and 2-deoxyglucose (2DG) in a pig model of chronic progressive ADPKD. EXPERIMENTAL APPROACH: A miniature pig ADPKD model was established by inducible deletion of the PKD1 gene. Blood, urine and kidney biopsy specimens were collected for analysis at specific times. The renal vesicle index was analysed by three-dimensional reconstruction of CT scans. Markers of the mammalian target of rapamycin (mTOR) and ERK signalling pathways and associated metabolism were detected by Western blots and colorimetry. KEY RESULTS: The three-dimensional reconstruction of CT scans indicated a markedly lower renal vesicle index in the combination therapy group. Each drug intervention group showed a significantly lower serum creatinine and urinary protein/creatinine ratio. This treatment regimen also inhibited the activities of markers of the proliferation-related mTOR and ERK pathways, and the expression of key enzymes involved in glycolysis, as well as reducing the production of ATP and lactic acid. CONCLUSIONS AND IMPLICATIONS: This study showed that the combination of metformin and 2DG blocked the formation of renal cysts and improved the renal function in ADPKD miniature pigs. Our results indicate that the combination of metformin and 2DG may be a promising therapeutic strategy in human ADPKD.

16.
Plant Cell ; 30(10): 2286-2307, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30309900

RESUMO

Sorghum (Sorghum bicolor) is the fifth most popular crop worldwide and a C4 model plant. Domesticated sorghum comes in many forms, including sweet cultivars with juicy stems and grain sorghum with dry, pithy stems at maturity. The Dry locus, which controls the pithy/juicy stem trait, was discovered over a century ago. Here, we found that Dry gene encodes a plant-specific NAC transcription factor. Dry was either deleted or acquired loss-of-function mutations in sweet sorghum, resulting in cell collapse and altered secondary cell wall composition in the stem. Twenty-three Dry ancestral haplotypes, all with dry, pithy stems, were found among wild sorghum and wild sorghum relatives. Two of the haplotypes were detected in domesticated landraces, with four additional dry haplotypes with juicy stems detected in improved lines. These results imply that selection for Dry gene mutations was a major step leading to the origin of sweet sorghum. The Dry gene is conserved in major cereals; fine-tuning its regulatory network could provide a molecular tool to control crop stem texture.


Assuntos
Variação Genética , Proteínas de Plantas/genética , Sorghum/genética , Fatores de Transcrição/genética , Parede Celular/genética , Parede Celular/metabolismo , Grão Comestível/genética , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Estudo de Associação Genômica Ampla , Haplótipos , Proteínas de Plantas/metabolismo , Caules de Planta/fisiologia , Seleção Genética , Sorghum/fisiologia
17.
Cell Physiol Biochem ; 48(6): 2258-2272, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30114693

RESUMO

BACKGROUND/AIMS: Non-small cell lung cancer (NSCLC) is one of the deadliest cancers worldwide. Dopamine receptor D2 (DRD2) has multiple roles in clinical progression of NSCLC and functional maintenance of cancer cells. However, little is known about the molecular mechanism. Here, we clarified whether DRD2 inhibits lung cancer progression and identified the underlying downstream signaling. METHODS: DRD2 mRNA and protein levels were detected in clinical specimens by qRT-PCR and immunohistochemistry, respectively. MTT and colony formation assays were applied to analyze cell proliferation. The underlying molecular mechanism was identified by dual luciferase, western blot, qRT-PCR, cAMP detection, immunoprecipitation, and chromatin immunoprecipitation assays. A murine NSCLC model was used to clarify the role of DRD2 in tumor cell proliferation. RESULTS: We found that DRD2 ablated tumor cell growth. DRD2 expression in NSCLC tissues was lower than in adjacent normal lung tissues. Moreover, DRD2 mRNA and protein levels in NSCLC were negatively correlated with the tumor size, TNM status, and patient overall survival. In vitro experiments showed that disruption of DRD2 promoted the proliferation of NSCLC cell lines A549 and SK-MES-1 by inhibiting the NF-κB signaling pathway. Furthermore, DRD2 overexpression not only blocked lipopolysaccharide-induced A549 and SK-MES-1 cell proliferation and growth, but also inhibited the tumorigenesis in murine xenograft models. CONCLUSION: These results indicate that DRD2 may be a potential therapeutic target for lung cancer patients with high DRD2 expression by ablating the NF-κB signaling pathway.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Pulmonares/patologia , Receptores de Dopamina D2/metabolismo , Animais , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Camundongos , Camundongos Nus , Pessoa de Meia-Idade , NF-kappa B/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Receptores de Dopamina D2/química , Receptores de Dopamina D2/genética , Transdução de Sinais , Fator de Transcrição RelA/genética , Fator de Transcrição RelA/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
18.
BMC Gastroenterol ; 18(1): 121, 2018 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-30071845

RESUMO

BACKGROUND: The vertical transmission of HBV from mothers to their infants at birth or in early infancy has a significant role in the endemicity of HBV infection. Tenofovir is one of the most potent anti-HBV agents with a high genetic barrier to resistance. The study is to evaluate the efficacy of tenofovir in preventing perinatal HBV transmission, as well as monitoring safety for mothers and infants. METHODS: PubMed, Embase, Web of Science, and CNKI (National Knowledge Infrastructure, China) database were systematically reviewed for studies that compared the efficacy and safety of tenofovir with other treatments. Pooled estimates were expressed with weight mean difference (WMD) with 95% confidence intervals (95% CIs) and risk ratio (RR) with 95% CIs. RESULTS: Nine studies involving 1046 pregnant patients met the inclusion criteria and were included in this meta-analysis. Compared with other treatments, tenofovir significantly reduced maternal HBV DNA levels (WMD = 2.33 log10 IU/mL, 95% CI: 1.01, 3.64; P < 0.001), infant HBsAg positivity rate (RR = 0.25, 95% CI: 0.16, 0.38; P < 0.001), infant HBeAg positivity rate (RR = 0.26, 95% CI: 0.14, 0.48; P < 0.001), infant HBV DNA positivity rate (RR = 0.15, 95% CI: 0.07, 0.31; P < 0.001), and immunoprophylaxis failure rate (RR = 0.31, 95% CI: 0.13, 0.73; P = 0.008). Moreover, maternal and infant safety profiles, including ALT, CK, and Cr were comparable between tenofovir and other treatment groups. CONCLUSION: Based on the current evidence, our study suggested that tenofovir significantly reduced the rate of vertical transmission of HBV, as well as the HBV DNA levels in HBV-infected mothers. Moreover, tenofovir was safe and tolerable for both mothers and their infants.


Assuntos
Antivirais/efeitos adversos , Antivirais/uso terapêutico , Hepatite B Crônica/transmissão , Transmissão Vertical de Doença Infecciosa/prevenção & controle , Complicações Infecciosas na Gravidez/prevenção & controle , Tenofovir/efeitos adversos , Tenofovir/uso terapêutico , Alanina Transaminase/sangue , Creatina Quinase/sangue , Creatinina/sangue , DNA Viral/sangue , Feminino , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/genética , Hepatite B Crônica/virologia , Humanos , Recém-Nascido , Gravidez , Complicações Infecciosas na Gravidez/virologia , Carga Viral
19.
J Cell Mol Med ; 22(9): 4328-4334, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29989303

RESUMO

We aimed to compare the diagnostic efficiency of proGRP and NSE on SCLC and to investigate whether the change of proGRP level would predict therapeutic response. Patients who were firstly diagnosed pathologically in Nanjing Chest Hospital and measured proGRP level consecutively were enrolled in the study. ProGRP level was detected using Elecsys ProGRP Assay. Totally 75 SCLC, 234 NSCLC and 264 benign lung diseases (BLD) were enrolled. Both proGRP and NSE levels in SCLC were significantly higher than those in NSCLC and BLD, and proGRP in extensive stage SCLC was higher than which in limited stage (P ≤ .001). The diagnostic efficiency of proGRP on SCLC was higher than that of NSE, but when the two biomarkers were bind together, the diagnostic efficiency was the best. When SCLC was differentiated from NSCLC and BLD, the cut-off values were 114.35 pg/mL and 162.55 pg/mL respectively. For treatment responsive patients, proGRP level decreased markedly after the first cycle of therapy and kept a continued momentum of decline during treatment. But for unresponsive patients, no obvious decline was observed. ProGRP had higher diagnostic efficiency on SCLC when compared to NSE, and it could better predict therapeutic response of pulmonary target lesions on chemotherapy.


Assuntos
Biomarcadores Tumorais/sangue , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Neoplasias Pulmonares/diagnóstico , Neoplasias/diagnóstico , Fragmentos de Peptídeos/sangue , Fosfopiruvato Hidratase/sangue , Carcinoma de Pequenas Células do Pulmão/diagnóstico , Adulto , Idoso , Antineoplásicos/uso terapêutico , Área Sob a Curva , Biomarcadores Tumorais/genética , Carcinoma Pulmonar de Células não Pequenas/sangue , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/patologia , China , Diagnóstico Diferencial , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Neoplasias/sangue , Neoplasias/patologia , Fragmentos de Peptídeos/genética , Fosfopiruvato Hidratase/genética , Proteínas Recombinantes/sangue , Proteínas Recombinantes/genética , Estudos Retrospectivos , Sensibilidade e Especificidade , Carcinoma de Pequenas Células do Pulmão/sangue , Carcinoma de Pequenas Células do Pulmão/tratamento farmacológico , Carcinoma de Pequenas Células do Pulmão/patologia
20.
Chem Commun (Camb) ; 54(55): 7673-7676, 2018 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-29938280

RESUMO

Herein, we develop a new nanopore sensing strategy for the selective detection of microRNAs and single nucleotide variants (SNVs) based on triplex molecular beacon sensors. This sensing system shows very high specificity in discriminating microRNA SNVs and can be applied for the simultaneous detection of several microRNAs of the same family in a mixture.


Assuntos
Sondas de DNA/genética , MicroRNAs/análise , MicroRNAs/genética , Polimorfismo de Nucleotídeo Único/genética , Técnicas Biossensoriais/métodos , Sondas de DNA/química , Limite de Detecção , Bicamadas Lipídicas/química , Nanoporos , Hibridização de Ácido Nucleico , Fosfatidilcolinas/química
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