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1.
Eur J Ophthalmol ; : 11206721211073035, 2022 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-35018839

RESUMO

PURPOSE: To evaluate the efficacy and safety of CO2-Laser Assisted Sclerectomy Surgery (CLASS) with 5-fluorouracil (5-FU) in treating open-angle glaucoma (OAG) in Chinese patients. Methods: This was a retrospective, uncontrolled, interventional case series. All patients from 2016 to 2017 who received CLASS were recruited in this study. The primary outcome was the change in intraocular pressure (IOP) and the number of IOP-lowering medications over a 12-month follow-up period. Adverse events were evaluated as secondary outcomes. Results: Data were collected from forty-two eyes of 31 patients. The average preoperative IOP was 31.33 ± 7.60mmHg. The mean percentage of IOP reduction from baseline at postoperative months (POM) 1, 3, 6, 9, and, 12 were 48.1% ± 24.6%, 51.4% ± 19.3%, 51.2% ± 17.2%, 50.9% ± 15.0%, 49.2% ± 16.3%, respectively (all P < 0.001). The number of glaucoma medications decreased from a baseline of 3.02 ± 0.81 to 0.05 ± 0.22, 0.10 ± 0.37, 0.12 ± 0.40, 0.17 ± 0.44, and 0.24 ± 0.58 at POM 1, 3, 6, 9, and 12, respectively (all P < 0.001). At POM 1, 3, 6, 9, and 12, complete success rates were 66.7%, 73.8%, 76.2%, 69.1%, and 71.4%, respectively. At POM 1, 3, 6, 9, and 12, qualified success rates were 71.4%, 82.0%, 85.3%, 83.3%, and 90.5%, respectively. Major postoperative complications include peripheral iris synechia, iris incarceration, and anterior chamber shallowing. Conclusions: CLASS with 5-FU shows safety and efficacy for decreasing IOP and the number of IOP-lowering medications over a 12-month follow-up period. It could be an alternative treatment for patients with OAG.

2.
Artigo em Inglês | MEDLINE | ID: mdl-35034424

RESUMO

Covalent organic frameworks (COF) possess a robust and porous crystalline structure, making them an appealing candidate for energy storage. Herein, we report an exfoliated polyimide COF composite (P-COF@SWCNT) prepared by an in-situ condensation of anhydide and amine on the single-walled carbon nanotubes as advanced anode for potassium-ion batteries (PIBs). Numerous active sites exposed on the exfoliated frameworks and the various open pathways promote the highly efficient ion diffusion in the P-COF@SWCNT while preventing irreversible dissolution in the electrolyte. During the charging/discharging process, K + is engaged in the carbonyls of imide group and naphthalene rings through the enolization and π-K + effect, which is demonstrated by the DFT calculation and XPS, ex-situ FTIR, Raman. As a result, the prepared P-COF@SWCNT anode enables incredibly high reversible specific capacity of 438 mA h g -1 at 0.05 A g -1 and extended stability. The structural advantage of P-COF@SWCNT enables more insights into the design and versatility of COF as an electrode.

3.
Phys Med Biol ; 2022 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-35042207

RESUMO

Incidence of primary thyroid cancer rises steadily over the past decades because of overdiagnosis and overtreatment through the improvement in imaging techniques for screening, especially in ultrasound examination. Metastatic status of lymph nodes is important for staging the type of primary thyroid cancer. Deep learning algorithms based on ultrasound images were thus developed to assist radiologists on the diagnosis of lymph node metastasis. To integrate more clinical context (e.g., health records and various image modalities) into, and explore more interpretable patterns discovered by, these algorithms, a deep multimodal learning network was developed in this study for the prediction of lymph node metastasis in primary thyroid cancer patients. The result indicates that the proposed network achieved an average F1 score of 0.888 and an average AUC value of 0.973 in two independent validation sets, and the performance was significantly better than three single-modality deep learning networks. Furthermore, a novel index was proposed to compare the contribution of different modalities in making the predictions, which showed that among three modalities used in this study, the deep multimodal learning network relied generally more on image modalities than the data modality of clinic records. Our work is beneficial to prospective clinic trials of the radiologists, and will better help them understand how the predictions are made in deep multimodal learning algorithms.

5.
Biomarkers ; : 1-27, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34989306

RESUMO

AIMS: Attention deficit/hyperactivity disorder (ADHD) is a prevalent disorder of neurodevelopment in children. The diagnosis of ADHD mainly relies on the symptoms and some may be misdiagnosed due to age-based variation in behaviours. This study aimed to explore biomarkers that are greatly needed for the accurate diagnosis of ADHD. METHODS: 742 samples were retrospectively investigated in 3 independent cohorts, screening, training, and validation, for circulation microRNA measurement using microarray, Taqman polymerase chain reaction and regression analysis. RESULTS: A panel of five miRNAs (miR-4516, miR-6090, miR-4763-3p, miR-4281 and miR-4466) were identified as ADHD independent risk factors that provided a high diagnostic accuracy and specificity of ADHD (AUC =0.940 and 0.927 in the training and validation datasets, respectively). This panel of miRNAs differentiated ADHD well from control groups. After clinical improvement by treatment, the panel of miRNAs in patients and AUC changed significantly, and were close to those in healthy controls. Importantly, the targets of the miRNAs identified were commonly enriched in receptor signalling pathways, ion channels and synapse structures. CONCLUSION: Our study identified a useful panel of miRNAs that have considerable clinical value in evaluating ADHD and provide important evidence for aberrant epigenetic regulation in ADHD.

6.
Phytochemistry ; 193: 112973, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34656025

RESUMO

Ten previously undescribed cassane diterpenoids, cassabonducins A-J, and eleven known compounds were isolated from the seeds of Caesalpinia bonduc. The structures of the undescribed compounds were elucidated by extensive analysis of spectroscopic data (IR, HRESIMS, and 1H, 13C and 2D NMR) and their absolute configurations were determined by the ECD data and single-crystal X-ray diffraction analysis. ε-Caesalpin-Ⅶ was obtained from natural resources for the first time. Cassabonducin A possessed noteworthy inhibitory activity against LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages with IC50 value of 6.12 µM. Cassabonducin D and neocaesalpin N showed moderate α-glucosidase inhibition at the concentration of 50 µM with inhibitory capacities of 47.17% and 43.83%, respectively.


Assuntos
Caesalpinia , Diterpenos , Diterpenos/farmacologia , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Óxido Nítrico , Sementes , alfa-Glucosidases
7.
Materials (Basel) ; 14(23)2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34885581

RESUMO

It is now more popular to use basalt fibers in the engineering programs to reinforce the crack resistance of asphalt mixtures. However, research concerning the impact of the basalt fiber diameter on the macro performance of AC-13 mixtures is very limited. Therefore, in this paper, basalt fibers with three diameters, including 7, 13 and 25 µm, were selected to research the influences of fiber diameter on the crack resistance of asphalt mixtures. Different types of crack tests, such as the low temperature trabecular bending test (LTTB), the indirect tensile asphalt cracking test (IDEAL-CT), and the semi-circular bend test (SCB), were conducted to reveal the crack resistance of AC-13 mixtures. The entire cracking process was recorded through the digital image correlation (DIC) technique, and the displacement cloud pictures, strain, average crack propagation rate (V) and fracture toughness (FT) indicators were used to evaluate the crack inhibition action of the fiber diameter on the mixture. The results showed that the incorporation of basalt fiber substantially improved the crack resistance, slowed down the increase of the displacement, and delayed the fracture time. Basalt fiber with a diameter of 7 µm presented the best enhancement capability on the crack resistance of the AC-13 mixture. The flexibility index (FI) of the SCB test showed a good correlation with V and FT values of DIC test results, respectively. These findings provide theoretical advice for the popularization and engineering application of basalt fibers in asphalt pavement.

8.
Arch Med Sci ; 17(6): 1752-1765, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34900057

RESUMO

Introduction: Accumulating evidence has revealed the critical roles of long noncoding RNAs (lncRNAs) in various cancers. LncRNA SNHG20 has been shown to be a cancer-associated lncRNA in several cancers with diverse mechanisms. However, the clinical references, biological roles, and mechanisms of action of SNHG20 in prostate cancer (PCa) are still unclear. Material and methods: The expression of SNHG20 in PCa tissues and cell lines was detected by RT-qPCR. The correlations between SNHG20 expression and clinicopathological features were analyzed by χ2 test. The roles of SNHG20 in PCa cell proliferation and migration were detected by CCK-8, EdU incorporation, and transwell assays. The regulatory mechanisms of SNHG20 on DDX17 were detected by dual luciferase reporter assay, RT-qPCR, and western blot. Results: SNHG20 is highly expressed in PCa tissues and cell lines. High expression of SNHG20 is positively correlated with high Gleason score and advanced tumor stage. Functional experiments revealed that overexpression of SNHG20 promotes PCa cell proliferation and migration. SNHG20 knockdown represses PCa cell proliferation and migration. Mechanistically, SNHG20 was verified to act as a competing endogenous RNA (ceRNA) to upregulate DDX17. DDX17 is also highly expressed and has oncogenic roles in PCa. Furthermore, the expression of DDX17 is significantly positively correlated with that of SNHG20 in PCa tissues. Depletion of DDX17 reverses the oncogenic roles of SNHG20 in PCa. Conclusions: These data showed that SNHG20 promotes PCa cell proliferation and migration via acting as a ceRNA to upregulate DDX17. This study also suggested that SNHG20 may be a potential novel therapeutic target for PCa.

9.
Chem Sci ; 12(45): 15054-15060, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34909145

RESUMO

Herein, a chemical-vapor deposition-like strategy was developed for the synthesis of versatile core-shell transition metal sulfide (TMS)@carbon nanowires with chemically-bonded heterostructures and significantly improved electrochemical performance. The morphological evolution observations revealed the simultaneous growth of TMS nanowires and their bonding with an ultrathin carbon layer. The resulting core-shell heterostructured nanowires possessed notable advantages, including fast ion/electron diffusion rates, improved conductivity, and chemical/mechanical stability, thereby leading to remarkable reversible capacity, rate capability, and cycling stability for Na-ion storage applications. The in situ transmission electron microscopy and in situ X-ray diffraction studies for FeS@C demonstrated the crystalline phase evolution between hexagonal and tetragonal FeS species during the electrochemical charging/discharging process, clearly indicating the excellent Na-ion storage performance of FeS@C nanowires. This work provides a new methodology for achieving 1D core-shell nanoarchitectures, while elucidating the electrochemical reaction mechanism underlying Na-ion storage in TMS materials.

11.
Adv Sci (Weinh) ; : e2104435, 2021 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-34923776

RESUMO

Developing orange to red purely organic luminescent materials having external quantum efficiencies (ηext s) exceeding 30% is challenging because it generally requires strong intramolecular charge transfer, efficient reverse intersystem crossing (RISC), high photoluminescence quantum yield (ΦPL ), and large optical outcoupling efficiency (Φout ) simultaneously. Herein, by introducing benzoyl to dibenzo[a,c]phenazine acceptor, a stronger electron acceptor, dibenzo[a,c]phenazin-11-yl(phenyl)methanone, is created and employed for constructing orange-red delayed fluorescence molecules with various acridine-based electron donors. The incorporation of benzoyl leads to red-shifted photoluminescence with accelerated RISC, reduced delayed lifetimes, and increased ΦPL s, and the adoption of spiro-structured acridine donors promotes horizontal dipole orientation and thus renders high Φout s. Consequently, the state-of-the-art orange-red organic light-emitting diodes are achieved, providing record-high electroluminescence (EL) efficiencies of 33.5%, 95.3 cd A-1 , and 93.5 lm W-1 . By referring the control molecule without benzoyl, it is demonstrated that the presence of benzoyl can exert significant positive effect over improving delayed fluorescence and enhancing EL efficiencies, which can be a feasible design for robust organic luminescent materials.

12.
Biochim Biophys Acta Gene Regul Mech ; 1865(1): 194777, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34843988

RESUMO

LncRNA (long noncoding RNA) is often dysregulated in tumors especially hepatocellular carcinoma (HCC). However, the dysregulation mechanism of lncRNAs is largely unknown. Here, we showed that lncRNA lncAY expression was stimulated in HCC by either endogenous or exogenous sulfatide. Elevated lncAY promoted HCC cell migration or angiogenesis, whereas lncAY silence suppressed HCC cell migration and proliferation. Interestingly, the activity of lncAY gene promoter was enhanced by sulfatide. Then Myb and MEF2C were identified as the transcription factors responsible for the stimulation of lncAY promoter activity and transcription by sulfatide. Both Myb and MEF2C enrichment on lncAY promoter was further confirmed, and their occupancy on lncAY promoter was strengthened by sulfatide for Myb or MEF2C was acetylated. Mutant Myb-K456A exhibited reduced acetylation and weak stimulation for lncAY transcription. However, Myb mutation K456/503A prevented Myb from acetylation induced by sulfatide. The mutant Myb K456/503A further was unable to occupy lncAY promoter and enhance lncAY transcription. In conclusion, this study demonstrated lncAY transcription was abnormally upregulated by sulfatide in HCC through Myb/MEF2C to promote HCC progression.

13.
Chem Biodivers ; 2021 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-34837325

RESUMO

Two new seco- prezizaane-type sesquiterpenes, 2 ß -hydroxy-6-deoxyneoanisatin ( 1 ) and 3,4-anhydro-2-oxo-1 α -hydroxy-6-deoxyneoanisatin ( 2 ), and two new prenylated C 6 -C 3 compounds, illilanceofunones A ( 3 ) and B ( 4 ), were obtained from the fruits of Illicium lanceolatum , along with four known prenylated C 6 -C 3 compounds ( 5-8 ). Their structures were proposed through HRESIMS, 1 H, 13 C, and 2D NMR data interpretation. Moreover, the absolute configuration of 1 and 2 were further assigned by single-crystal X-ray diffraction analysis and electronic circular dichroism (ECD) calculations, respectively .  Illihenryipyranol A ( 6 ) exhibited neuroprotective activity against MPP + -induced PC12 cell damage in a dose-dependent manner .

14.
Nanotechnology ; 33(8)2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34787098

RESUMO

Controllable tailoring and understanding the phase-structure relationship of the 1T phase two-dimensional (2D) materials are critical for their applications in nanodevices. Thein situtransmission electron microscope (TEM) could regulate and monitor the evolution process of the nanostructure of 2D material with atomic resolution. In this work, a controllably tailoring 1T-CrTe2nanopore is carried out by thein situTEM. A preferred formation of the 1T-CrTe2border structure and nanopore healing process are studied at the atomic scale. The controllable tailoring of the 1T phase nanopore could be achieved by regulating the transformation of two types of low indices of crystal faces {101¯0} and {112¯0} at the nanopore border. Machine learning is applied to automatically process the TEM images with high efficiency. By adopting the deep-learning-based image segmentation method and augmenting the TEM images specifically, the nanopore of the TEM image could be automatically identified and the evaluation result of DICE metric reaches 93.17% on test set. This work presents the unique structure evolution of 1T phase 2D material and the computer aided high efficiency TEM data analysis based on deep learning. The techniques applied in this work could be generalized to other materials for controlled nanostructure regulation and automatic TEM image analyzation.

15.
Front Microbiol ; 12: 769965, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777323

RESUMO

Background: Herbivorous insects are one of the main biological threats to crops. One such group of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are damaging to the quality of the seed yield as they feed on green developing seeds leading to poorly marketable harvests. In addition to causing physical damage during sucking-feeding activities, the insects can also transmit microbial pathogens, leading to even greater yield loss. Conducting surveys of the insect intestinal microbiome can help identify possible pathogens, as well as detail what healthy stink bug digestive systems have in common. Methods: We used the conserved V4 region of the 16S rRNA gene to characterize the bacterial microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil. Results: After quality filtering of the data, 192 samples were kept for analyses: 117 samples from P. guildinii covering three sites in Brazil and four sites in the United States, and 75 samples for E. heros covering 10 sites in Brazil. The most interesting observations were that the diversity and abundance of some bacterial families were different in the different ecoregions of Brazil and the United States. Conclusion: Some families, such as Acetobacteraceae, Bacillaceae, Moraxellaceae, Enterobacteriaceae, and Rhodocyclaceae, may be related to the better adaptation in some localities in providing nutrients, break down cellulose, detoxify phytochemicals, and degrade organic compounds, which makes it difficult to control these species.

16.
Artigo em Inglês | MEDLINE | ID: mdl-34815081

RESUMO

Lithium (Li) metal is deemed as an ideal and promising star anode for high energy storage but its application still is impeded due to uncontrollable Li dendrite growth and tremendous dimension change. Although the flexible and conductive three-dimensional (3D) skeleton can improve the structural and interfacial stability of Li anode, its inherently lithiophobic feature usually brings a high nucleation barrier, uneven Li+ flux, and large concentration polarization, leading to inhomogeneous Li plating/stripping. Here, we develop target material (denoted as Mo2C NPs@CC) consisting of well-distributed molybdenum carbide nanoparticles (Mo2C NPs) with intrinsic lithiophilicity serving as lithiophilic seeds implanted onto the carbon cloth, breaking the dilemma of ordinary 3D conductive skeletons. The Mo2C NPs with large Li absorption energy provide plentiful lithiophilic sites for guiding the uniform and thin Li-nuclei layer formation, thereby realizing flat Li growth and stable electrode/electrolyte interface. Moreover, the high electronic conductivity of Mo2C-modified 3D scaffolds can balance the lithiophilicity, ensuring the fast electron transport in the whole electrode, effectively lowering the local current density, and providing enough space for buffering volume change, and synergistically suppresses the growth of Li dendrites. As a result, a prolonged lifespan of 5000 cycles with low voltage hysteresis of 10 mV at current density of 2 mA cm-2 with area capacity (Ca) of 1 mA h cm-2 has been achieved, giving rational guidance for designing high-performance composite Li anodes.

17.
Molecules ; 26(21)2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34770935

RESUMO

Catharanthus roseus is a well-known traditional herbal medicine for the treatment of cancer, hypertension, scald, and sore in China. Phytochemical investigation on the twigs and leaves of this species led to the isolation of two new monoterpene indole alkaloids, catharanosines A (1) and B (2), and six known analogues (3-8). Structures of 1 and 2 were established by 1H-, 13C- and 2D-NMR, and HREIMS data. The absolute configuration of 1 was confirmed by single-crystal X-ray diffraction analysis. Compound 2 represented an unprecedented aspidosperma-type alkaloid with a 2-piperidinyl moiety at C-10. Compounds 6-8 exhibited remarkable Cav3.1 low voltage-gated calcium channel (LVGCC) inhibitory activity with IC50 values of 11.83 ± 1.02, 14.3 ± 1.20, and 14.54 ± 0.99 µM, respectively.

18.
Molecules ; 26(21)2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34771012

RESUMO

Naringin is a kind of multi-source food additive which has been explored broadly for its various biological activities and therapeutic potential. In the present study, the protective effect and mechanism of naringin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice were investigated. The results showed that naringin significantly alleviated DSS-induced colitis symptoms, including disease activity index (DAI), colon length shortening, and colon pathological damage. The tissue and serum secretion of inflammatory cytokines, as well as the oxidative stress, were decreased accordingly upon naringin intervention. Naringin also decreased the proteins involved in inflammation and increased the expression of tight junction (TJ) proteins. Moreover, naringin increased the relative abundance of Firmicutes/Bacteroides and reduced the content of Proteobacteria to improve the intestinal flora disorder caused by DSS, which promotes the intestinal health of mice. It was concluded that naringin can significantly ameliorate the pathogenic symptoms of UC through inhibiting inflammatory response and regulating intestinal microbiota, which might be a promising natural therapeutic agent for the dietary treatment of UC and the improvement of intestinal symbiosis.

19.
Chin Med ; 16(1): 117, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34774080

RESUMO

BACKGROUND: Irritable bowel syndrome (IBS) is a common gastrointestinal functional disease. Adults with IBS may experience abdominal pain, change of bowel habits, and abnormal stool form without organic disease. IBS can seriously affect their work productivity and quality of life, especially diarrhea-predominant irritable bowel syndrome (IBS-D). The Chinese medicine JCM-16021 has been shown to be potentially effective in improving the symptoms of IBS-D based on a small scale clinical trial. Hence, a large scale clinical study is designed to further evaluate the efficacy and safety of the Chinese medicine JCM-16021 for IBS-D with traditional Chinese medicine (TCM) pattern of Liver Stagnation and Spleen Deficiency (LSSD). METHODS: This study is a multi-center, randomized, double-blind, placebo-controlled clinical trial. 392 eligible participants will be enrolled with 2-week run-in, 8-week treatment and 8-week follow-up. After run-in period, participants will be randomized to receive either the Chinese medicine JCM-16021 or placebo for 8 weeks, and will have post-treatment follow up for another 8 weeks. The primary outcome is the improvement rate on the global assessment of improvement (GAI) at week 10. The secondary outcomes consist of changes of IBS-D symptoms, TCM pattern improvement, IBS-Quality of Life (IBS-QoL), IBS-Symptom Severity Score (IBS-SSS), safety, etc. RESULTS: A standard protocol has been developed for the study. The protocol will provided a detailed procedure to conduct a clinical trial and verify if the Chinese medicine JCM-16021 would significantly improve the overall symptoms of IBS-D with LSSD pattern of TCM by relieving abdominal pain, reducing stool frequency, improving the stool consistency and improving quality of life. The consolidated evidence from the study can shed light on the treatment of IBS-D with Chinese medicine. CONCLUSION: The protocol will provide details for investigators about the study following SPIRIT Statement. High-quality evidence on the efficacy and safety of Chinese medicine JCM-16021 for IBS-D will be provided through strict compliance with the protocol. TRIAL REGISTRATION: ClinicalTrial.gov identifier: NCT03457324. Registered 8 February 2018, https://clinicaltrials.gov/ct2/show/NCT03457324?term=NCT03457324&draw=2&rank=1.

20.
Hum Vaccin Immunother ; : 1-14, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34756160

RESUMO

Increased severity of diseases caused by Coxsackievirus A10 (CV-A10) as well as a large number of mutants and recombinants circulating in the population are a cause of concern for public health. A vaccine with broad-spectrum and homogenous protective capacity is needed to prevent outbreaks of CV-A10. Here, we evaluated cross-neutralization of prototype strain and 17 CV-A10 strains from related manufacturers in mainland China in vitro using 30 samples of plasma collected from naturally infected human adults and 18 sera samples from murine immunized with the above strains of CV-A10. Both human plasma and murine sera exhibited varying degrees of cross-neutralizing activities. Prototype A/Kowalik and sub-genotype C3/S113 were most difficult to neutralize. Among all strains tested, neutralization of S102 and S108 strains by 18 different sera was the most uniform, suggesting their suitability for detection of NtAb titers of different vaccines for avoiding biases introduced by detection strain. Furthermore, among all immune-sera, cross-neutralization of the 18 strains of CV-A10 by anti-S110 and anti-S102 was the most homogenous. Anti-S102 exhibiting higher geometric mean titer (GMT) in vitro was evaluated for its cross-protection capacity in vivo. Remarkably, administration of anti-S102 protected mice from lethal dosage of eight strains of CV-A10. These results provide a framework for formulating strategies for the R&D of vaccines targeting CV-A10 infections.

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