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1.
J Dev Orig Health Dis ; : 1-11, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31679538

RESUMO

Maternal supraphysiological estradiol (E2) environment during pregnancy leads to adverse perinatal outcomes. However, the influence of oocyte exposure to high E2 levels on perinatal outcomes remains unknown. Thus, a retrospective cohort study was conducted to explore the effect of high E2 level induced by controlled ovarian stimulation (COH) on further outcomes after frozen embryo transfer (FET). The study included all FET cycles (n = 10,581) between 2014 and 2017. All cycles were categorized into three groups according to the E2 level on the day of the human Chorionic Gonadotropin trigger. Odds ratios (ORs) and their confidence intervals (CIs) were calculated to evaluate the association between E2 level during COH and pregnancy outcomes and subsequent neonatal outcomes. From our findings, higher E2 level was associated with lower percentage of chemical pregnancy, clinical pregnancy, ongoing pregnancy, and live birth as well as increased frequency of early miscarriage. Preterm births were more common among singletons in women with higher E2 level during COH (aOR1 = 1.93, 95% CI: 1.22-3.06; aOR2 = 2.05, 95% CI: 1.33-3.06). Incidence of small for gestational age (SGA) was more common in both singletons (aOR1 = 2.01, 95% CI: 1.30-3.11; aOR2 = 2.51, 95% CI: 1.69-3.74) and multiples (aOR1 = 1.58, 95% CI: 1.03-2.45; aOR2 = 1.99, 95% CI: 1.05-3.84) among women with relatively higher E2 level. No association was found between high E2 level during COH and the percentage of macrosomia or large for gestational age. In summary, oocyte exposure to high E2 level during COH should be brought to our attention, since the pregnancy rate decreasing and the risk of preterm birth and SGA increasing following FET.

2.
J Dermatolog Treat ; : 1-11, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31689168

RESUMO

Background: The application of fractional Q-switched ruby laser (FQSRL) or intense pulsed light (IPL) on Café-au-lait macule (CALM) is rational and the data is lacking. Objective: To evaluate the efficacy and safety of FQSRL and IPL in CALM. Methods: The patients with CALM who were treated with FQSRL or IPL were retrospectively observed from April 2016 to April 2019. The laser/light treatments were conducted at an interval of 3-4 weeks. Results: For FQSRL (N = 67), 88.23%, 95.46%, 100% patients achieved >50% improvement by 3 sessions, 4 sessions, and more than 4 sessions of treatment, respectively. A better and better efficacy was shown with the increasing number of sessions (χ2=89.51, p < 0.01). For IPL (N = 54), 45% and 87.5% achieved >50% improvement by 3 sessions and more than 4 sessions of treatments, respectively. More than 4 sessions achieved better efficacy than less sessions (p < 0.01). Under various time-points, FQSRL presented more favorable responses than IPL (p<0.05). All the adverse effects were tolerable and acceptable. Conclusion: FQSRL or IPL would be an alternative and safe modality for CAML in Chinese patients.

3.
Biomater Sci ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31691698

RESUMO

Titanium (Ti)-based implants often suffer from detrimental bacterial adhesion and inefficient healing, so it is crucial to design a dual-functional coating that prevents bacterial infection and enhances bioactivity for a successful implant. Herein, we successfully devised a cationic polypeptide (Pep)-functionalized biomimetic nanostructure coating with superior activity, which could not only kill pathogenic bacteria rapidly and inhibit biofilm formation for up to two weeks, but also promote in situ hydroxyapatite (HAp) formation. Specifically, a titania (TiO2) nanospike coating (TNC) was fabricated by alkaline hydrothermal treatment firstly, followed by immobilization of rationally synthesized Pep via robust coordinative interactions, named TNPC. This coating was able to effectively kill (>99.9%) both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria, while being non-toxic to murine MC3T3-E1 osteoblastic cells. Furthermore, the in vivo infection studies denoted that the adherent bacteria numbers on the TNPC implants were significantly reduced by 6 orders of magnitude than those on the pure Ti implants (p < 0.001). Importantly, in the presence of cationic amino groups and residual Ti-OH groups, substantial HAp deposition on the TNPC surface in Kokubo's simulated body fluid (SBF) occurred after 14 days. Altogether, our results support the clinical potential of this biomimetic dual-functional coating as a new approach with desirable antibacterial properties and HAp-forming ability in orthopedic and dental applications.

4.
J Crohns Colitis ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31693091

RESUMO

BACKGROUND AND AIMS: CD39/ENTPD1 scavenges pro-inflammatory nucleotides, to ultimately generate immunosuppressive adenosine, having a central role in immune-homeostasis. Global deletion of Cd39 increases susceptibility to experimental colitis while single-nucleotide-polymorphisms within the human CD39 promoter, and aberrant patterns of expression during experimental hypoxia, predispose to Crohn's disease. We aimed to define the impact of transgenic-human-CD39 (hTG) overexpression in experimental colitis and to model therapeutic effects using the recombinant apyrase APT102 in vivo. We also determined in vitro effects of APT102 on phenotypic and functional properties of regulatory T-lymphocytes derived from patients with Crohn's disease. METHODS: Colitis was induced upon administration of dextran-sulfate-sodium in wild-type (WT) or hTG mice; and, in another model, by adoptive transfer of CD45RBhigh-cells with or without WT or hTG-Treg. In additional experiments, mice were treated with APT102. Effects of APT102 on phenotype and function of Treg and type-1-regulatory-T (Tr1)-cells were also evaluated, after purification from peripheral blood and lamina propria of Crohn's patients (n=38). RESULTS: Overexpression of human CD39 attenuated experimental colitis and protected from deleterious effects of systemic hypoxia, pharmacologically induced by deferoxamine. Administration of APT102 in vivo enhanced the beneficial effects of endogenous Cd39 boosted by the administration of the aryl-hydrocarbon-receptor (AhR) ligand unconjugated bilirubin (UCB). Importantly, supplemental APT102 restored responsiveness to AhR stimulation by UCB in Treg and Tr1-cells, obtained from Crohn's patients. CONCLUSIONS: hCD39 overexpression ameliorated experimental colitis and prevented hypoxia-related damage in vivo. Exogenous administration of APT102 boosted AhR-mediated regulatory effects in vivo while enhancing Treg functions in Crohn's disease in vitro.

5.
PLoS One ; 14(11): e0224601, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31703068

RESUMO

Male-specifically inherited Y-STRs, harboring the features of haploidy and lack of crossing over, have gained considerable attention in population genetics and forensic investigations. Goldeneye® Y-PLUS kit was a recently developed amplification system focused on the genetic diversity of 36 Y-chromosomal short tandem repeats (Y-STRs) in East Asians. However, no population data and corresponding forensic features were reported in China. Here, 36 Y-STRs were first genotyped in 400 unrelated healthy Tai-Kadai-speaking Bouyei male individuals. A total of 371 alleles and 396 haplotypes could be detected, and the allelic frequencies ranged from 0.0025 to 0.9875. The haplotype diversity, random match probability and discrimination capacity values were 0.9999, 0.0026 and 0.9900, respectively. The gene diversity (GD) of 36 Y-STR loci in the studied group ranged from 0.0248 (DYS645) to 0.9601 (DYS385a/b). Population comparisons between the Guizhou Bouyei and 80 reference groups were performed via the AMOVA, MDS, and phylogenetic relationship reconstruction. The results showed that the population stratification was almost consistent with the geographic distribution and language-family, both among Chinese and worldwide ethnic groups. Our newly genotyped Bouyei samples show a close affinity with other Tai-Kadai-speaking groups in China and Southeast Asia. Our data may provide useful information for paternal lineage in the forensic application and population genetics, as well as evidence for archaeological and historical research.

6.
BMC Cancer ; 19(1): 1041, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690274

RESUMO

BACKGROUND: The ultimate goal of locoregional therapy (LRT) to the liver is to induce total tumor necrosis. Trans-arterial chemoembolization (TACE) is the mainstay bridging therapy for patients with hepatocellular carcinoma (HCC) waiting for liver transplantation (LT). However, tumor response rate is variable. The purpose of this study was to correlate HCC radiological appearance with level of tumor necrosis during explant analysis from patients undergoing LT who received pre-LT TACE. METHODS: From January 2000 to December 2018, a total of 66 patients with HCC who had been treated prior to LT by means of TACE were analyzed. Diagnosis of HCC was made based on AASLD guidelines and confirmed via histopathology explant analysis. Radiologic tumor response after TACE was based on modified Response Evaluation Criteria in Solid Tumors (mRECIST). Degree of tumor necrosis was determined by histopathology analysis of liver explants. HCC radiological appearances on CT before TACE were assessed and correlated with histological findings after LT. RESULTS: Eighty nine TACE procedures (1.35 ± 0.67; 1-4) were performed, of which 18 were repeated TACE (27.3%) procedures. In 56.1% of the patients, ≥90% (near-complete) tumor necrosis was achieved. Concordance between mRECIST criteria and pathology was observed in 63% of the patients, with an underestimation of tumor response in 18 (27%) patients and an overestimation in 6 (9.1%). Near-complete tumor necrosis upon pathological analysis was associated with tumor hyper-enhancement in the arterial phase (P = 0.002), "typical tumor enhancement" (P = 0.010) and smooth tumor margins (p = 0.011). The multivariate analysis showed that well circumscribed HCCs with smooth margins and arterial hyper-enhancement independently correlated with post-TACE near-complete histological tumor necrosis. CONCLUSIONS: The well circumscribed HCC lesions with arterial hyper-enhancement are more susceptible to TACE than lesions with arterial phase iso or hypo-enhancement and lesions with infiltrative appearance. Pre-TACE CT imaging may ease the selection of an optimal treatment strategy for bridging patients with HCC to liver transplantation.

7.
Parasit Vectors ; 12(1): 520, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690347

RESUMO

BACKGROUND: Cysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions. METHODS: In the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5). RESULTS: The median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep. CONCLUSIONS: To the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.

8.
Chem Biodivers ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705573

RESUMO

A facile method was developed for synthesis of boronic acid-functionalized silica nanocomposites (SiO 2 -BA) by "thiol-ene"click reaction, where silica nanoparticles were synthesized by using tetraethoxysilane (TEOS) and γ-mercaptopropyl trimethoxysilane (γ-MPTS) as precursors. The morphology and structure properties of the resultant SiO 2 -BA were characterized by Transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR),and Brunner-Emmet-Teller measurements  (BET). The adsorption behavior of the SiO 2 -BA for glycoproteins was evaluated. Under the optimized conditions, the SiO 2 -BA exhibited higher adsorption capacity towards glycoproteins (ovalbumin, OVA, (7.64 µmol/g) than non-glycoproteins (bovine serum albumin,BSA, 0.83 µmol/g). In addition, the practicality of the SiO 2 -BA was further assessed by selective enrichment of glycophoproteins from egg white samples.

9.
J Cell Mol Med ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31675186

RESUMO

Inflammation and neuronal apoptosis contribute to the progression of secondary injury after spinal cord injury (SCI) and are targets for SCI therapy; autophagy is reported to suppress apoptosis in neuronal cells and M2 polarization may attenuate inflammatory response in microglia, while both are negatively regulated by mTORC1 signalling. We hypothesize that mTORC1 suppression may have dual effects on inflammation and neuronal apoptosis and may be a feasible approach for SCI therapy. In this study, we evaluate a novel inhibitor of mTORC1 signalling, Astragaloside IV (AS-IV), in vitro and in vivo. Our results showed that AS-IV may suppress mTORC1 signalling both in neuronal cells and microglial cells in vitro and in vivo. AS-IV treatment may stimulate autophagy in neuronal cells and protect them against apoptosis through autophagy regulation; it may also promote M2 polarization in microglial cells and attenuate neuroinflammation. In vivo, rats were intraperitoneally injected with AS-IV (10 mg/kg/d) after SCI, behavioural and histological evaluations showed that AS-IV may promote functional recovery in rats after SCI. We propose that mTORC1 suppression may attenuate both microglial inflammatory response and neuronal apoptosis and promote functional recovery after SCI, while AS-IV may become a novel therapeutic medicine for SCI.

10.
Radiat Oncol ; 14(1): 191, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31684983

RESUMO

BACKGROUND: Re-irradiation (re-RT) has the active effect of relieving clinical symptoms and prolonging the survival of patients with recurrent esophageal squamous cell carcinoma (ESCC). However, the optimal re-RT dose is still uncertain. Here, we analyzed the prognostic factors associated with survival and explored the optimal re-RT dose for patients with recurrent ESCC following definitive radiotherapy. PATIENTS AND METHODS: The data of 47 patients with recurrent ESCC who were retreated between 2010 and 2014 were retrospectively analyzed. All patients received a radiation dose > 50 Gy during the primary treatment. The median time to recurrence after primary radiotherapy was 26 months (range 6-120 months). All patients had in-field recurrence in the esophagus. Recurrence within the local site was observed in 37 patients (78.7%), and recurrence in both the local site and regional nodes were observed in 10 patients (21.3%). All patients received 3D conformal re-RT with a median dose of 58 Gy (range 26-64 Gy). Chemotherapy was sequentially used in 27.7% of the patients. Survival curves were constructed according to the Kaplan-Meier method and were compared by log-rank tests. The factors predictive of survival were identified with univariate and multivariate analyses. RESULTS: Dysphagia relief after re-RT was achieved in 20 of the 35 symptomatic patients (57.1%). The median survival time (MST) of all patients was 17 months, and the 1-, 2-, 3- and 5-year overall survival (OS) rates were 72.3, 25.5, 17.0 and 2.1%, respectively. In the univariate analysis, an Eastern Cooperative Oncology Group Performance Status (ECOG-PS) of 0-1 (P = 0.014), recurrence at the local site (P = 0.048), time to recurrence ≥24 months (P = 0.006) and re-RT dose ≥50 Gy (P < 0.001) were associated with favorable OS. In the multivariate analysis, only re-RT dose was an independent factor for OS (P = 0.007). Severe complications were observed in 7 patients, two of whom received a re-RT dose > 60 Gy. CONCLUSION: Our results demonstrated that patients with recurrent ESCC following definitive radiotherapy had unfavorable OS. Re-RT could be considered a feasible and effective treatment modality. A re-RT dose > 50 Gy could improve the survival outcomes, and a dose > 60 Gy should be administered with caution due to the risk of severe complications.

11.
Neurosci Lett ; : 134605, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31698028

RESUMO

OBJECTIVE: This study aims to assess the effects of physical training based on the functional near-infrared spectroscopy (fNIRS) and heart rate signals. METHODS: The oxygenated hemoglobin concentration (Delta [HbO2]) signals were recorded from the left prefrontal cortex (LPFC), right prefrontal cortex (RPFC), left motor cortex (LMC) and right motor cortex (RMC) of 23 subjects with methamphetamine (METH) dependencies at resting, spinning training and strength training states. The wavelet phase coherence (WPCO) values were calculated in four frequency intervals: I, 0.6-2; II, 0.145-0.6; III, 0.052-0.145; and IV, 0.021-0.052 Hz. During the spinning training and strength training states, heart rate signals were recorded at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 minutes, respectively. RESULTS: After physical training, the brain regions of LPFC, RPFC and LMC showed different degrees of activation in the subjects with METH dependencies (p < 0.05). The WPCO values between the brain regions significantly altered after spinning training and strength training (p < 0.05) in frequency intervals I, II, III and IV. CONCLUSIONS: The altered WPCO values indicated physical training could affect brain functional connectivity (FC) to a certain extent in the subjects with METH dependencies. These findings provide a method for the assessment of the effects of physical training in FC and will contribute to the development of drug rehabilitation methods in subjects with METH dependencies.

12.
Math Biosci Eng ; 16(6): 6975-6989, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31698599

RESUMO

The traditional path optimization problem is to consider the shortest path of the vehicle, but the shortest path does not effectively reduce the logistics cost. On the contrary, in the case of one-sided pursuit of the shortest path, it may cause some negative effects. This paper constructs a more realistic path optimization model on the path of traditional logistics distribution, and designs a path model based on simulated annealing algorithm which taking fuel consumption, cost, road gradient and condition of vehicle into account. The algorithm model of load capacity and other problems is used to verify the algorithm of the model through a simulation case of multiple distribution points. The experimental results show that the path optimization strategy considering the gradient of the road reduces the cost of the vehicle path, indicating the correctness of considering the vehicle load and road gradient factors in logistics transportation.

13.
J Exp Bot ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31701152

RESUMO

The critical functions of 2-oxoglutarate-dependent dioxygenase (2OGD) for the metabolism of salicylic acid (SA) in plants have been demonstrated, yet, the role of 2OGD in SA biosynthesis is poorly understood. Here, we report that two dioxygenase-encoding genes, SLC1 (slender and crinklyleaf 1) and SLC2 (slender and crinklyleaf 2), play essential roles in shoot development and SA production in rice (Oryza sativa). Overexpression of SLC1 (SLC1-OE) or SLC2 (SLC2-OE) in rice produced bizarre infertile plants with slender and crinkly leaves. Disruption of SLC1 or SLC2 led to dwarf plants. Down-regulation of SLC1 and SLC2 at the same time in the SLC1-SLC2 RNAi lines resulted in a severe defect in early leaf development. Enhanced SA level in the SLC1-OE plants and decreased SA level in the slc1 and slc2 mutants were scored. Accordingly, altered expression level of a set of SA-related genes in plants of SLC1-OE, slc1 and slc2 mutants was observed. The results from exploring the putative interacting proteins for SLC1 indicated that, either the KNOX1- or ELK-domain of OSH1 (homeobox1) is sufficient for accomplishing the interaction between SLC1 and OSH1. Collectively, our data revealed the importance of SLC1 and SLC2 in the shoot development of rice.

14.
Int J Cancer ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31671209

RESUMO

Melanoma is an aggressive form of skin cancer, and a worldwide problem with increasing incidence. Little is known about the burden of melanoma in Chinese population. We evaluated temporal trends and geographic variation in melanoma-associated burden, to narrow an important knowledge gap concerning the consequences of this disorder across time, provinces in China. Following the general analytic strategy used in the 2017 Global Burden of Disease study, we analyzed the incidence, mortality, prevalence and disability-adjusted life-years (DALYs) of melanoma, by age, sex and geography from 1990 to 2017. Levels in melanoma burden were assessed for 33 province-level administrative units between 1990 and 2017. We used joinpoint regression analysis to estimate the slope of incidence and mortality trends. The age-standardized incidence rate of melanoma was 0.9 per 100,000 in 2017, with a 110.3% rise compared with 1990. Although the age-standardized DALYs rate (per 100,000) decreased from 9.1 in 1990 to 7.6 in 2017, it showed an upward trend from 2007 to 2017. The DALYs rate increased steadily with age for females while increased and peaked at 55-59 years for males. The incidence of melanoma was higher in the clustered eastern provinces than western provinces, while the DALYs showed a pattern in opposite direction. In China, there has been a substantial increase in the burden of melanoma over the last decade, representing an ongoing challenge in Chinese population. More targeted strategies should be developed for elderly population, especially for females, to reduce the melanoma burden throughout China, particularly the western provinces. This article is protected by copyright. All rights reserved.

15.
Cell ; 179(2): 417-431.e19, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31585081

RESUMO

Severe asthma patients with low type 2 inflammation derive less clinical benefit from therapies targeting type 2 cytokines and represent an unmet need. We show that mast cell tryptase is elevated in severe asthma patients independent of type 2 biomarker status. Active ß-tryptase allele count correlates with blood tryptase levels, and asthma patients carrying more active alleles benefit less from anti-IgE treatment. We generated a noncompetitive inhibitory antibody against human ß-tryptase, which dissociates active tetramers into inactive monomers. A 2.15 Å crystal structure of a ß-tryptase/antibody complex coupled with biochemical studies reveal the molecular basis for allosteric destabilization of small and large interfaces required for tetramerization. This anti-tryptase antibody potently blocks tryptase enzymatic activity in a humanized mouse model, reducing IgE-mediated systemic anaphylaxis, and inhibits airway tryptase in Ascaris-sensitized cynomolgus monkeys with favorable pharmacokinetics. These data provide a foundation for developing anti-tryptase as a clinical therapy for severe asthma.

16.
Arch Virol ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31595357

RESUMO

The phage vB_BthS-HD29phi infecting Bacillus thuringiensis strain HD29 was isolated and purified. The morphology of the phage showed that it belongs to the family Siphoviridae. The phage genome was 32,181 bp in length, comprised linear double-stranded DNA with an average G + C content of 34.9%, and exhibited low similarity to known phage genomes. Genomic and phylogenetic analysis revealed that vB_BthS-HD29phi is a novel phage. In total, 50 putative ORFs were predicted in the phage genome, and only 18 ORFs encoded proteins with known functions. This article reports the genome sequence of a new tailed phage and increases the known genetic diversity of tailed phages.

17.
Eur J Pharm Sci ; 141: 105110, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31654757

RESUMO

BACKGROUND: Allergic contact dermatitis (ACD) is a highly prevalent inflammatory and immune skin disease accompanied with persistent pruritus and pain. Oxymatrine (OMT) exhibits antipruritic and anti-inflammatory effects in squaric acid dibutyl ester (SADBE) induced ACD mice model, but the need for frequent administration stipulated by short half-life and low bioavailability limits clinical application. OBJECTIVE: To evaluate the analgesic and antipruritic effects of OMT gel (OG), OMT sustained release microgel powder (OMP) and OMT sustained release microgel cream (OMC) in SADBE induced ACD mice, with subsequent study of the mechanism and side effects (irritation) of optimal dosage form. METHOD: On day 11, the thickness of the right cheek skin of mice was measured and mice spontaneous behaviors were recorded for 1.5 h. In the OMC experiment, hematoxylin-eosin and toluidine blue staining were performed on the cheek skin, and the irritation of OMC was tested on the back skin of rabbits. Blood analyzer was used to measure the counts of inflammatory cells in peripheral blood. The mRNA expressions of IL-1ß, TNF-α, CXCR3, CXCL10, IL-6, IL-10, IL-17A and IL-31 in cheek skin, TRPA1 and TRPV1 channels in trigeminal ganglion (TG), IFN-γ in spleen and IL-17A in thymus were measured by RT-qPCR. RESULTS: OMC, OMP and OG significantly decreased wipes and scratching bouts, alleviated skin inflammation. OMC required less frequent administration and is easier to apply, while its antipruritic effect was stronger than the analgesic effect. OMC rescued the deficits in epidermal keratinization and inflammatory cell infiltration, decreased the leukocyte count in peripheral blood, had no irritation to the broken rabbit's skin. Furthermore, OMC significantly down-regulated the mRNA expression of IL-1ß, TNF-α, CXCR3, CXCL10, IL-6, IL-10, IL-17A and IL-31 in cheek skin, TRPA1 and TRPV1 channels in TG, IFN-γ in thymus and IL-17A in spleen. CONCLUSION: We have demonstrated that OMC exhibits advanced analgesic, antipruritic and anti-inflammatory effects when compared with OG and OMP in ACD mice by regulating inflammation, chemokines, immune mediators and inhibiting the mRNA expression of TRPA1 and TRPV1. OMC has no irritation to the intact and damaged skin of rabbits.

18.
Chemosphere ; 241: 124927, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31590029

RESUMO

Plasma treated g-C3N4 (PT-g-C3N4) was obtained by a simple and rapid DBD plasma modification process on the pristine g-C3N4. Compared with the pristine g-C3N4, the grain size of the PT-g-C3N4 decreased from 99.2 nm to 57.2 nm, the specific surface area and the pore volume increased by 15% and 33.8%, respectively. Oxygen-containing groups such as -NO2 and -COOH were observed to form on the surface of PT-g-C3N4 so the hydrophilic property of PT-g-C3N4 was much higher than that of pristine g-C3N4. More importantly, the photocatalytic H2O2 production activity of PT-g-C3N4 was significantly improved on account of the treatment in plasma atmosphere for only 5 min, the H2O2 yield of which was about 13 times that of the pristine g-C3N4. Our finding is not only of great significance for effectively promoting the production of H2O2 under mild conditions, but also proposes an innovative DBD plasma method to modify the g-C3N4 photocatalyst, which effectively promotes the improvement of photocatalytic activity and provides valuable insights for catalyst modification studies.

19.
Chem Commun (Camb) ; 55(85): 12805-12808, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31595274

RESUMO

A new silver-mediated 1,2-alkylesterification of alkenes with nitriles and acids promoted by a catalytic amount of nickel catalyst for producing acyloxylated nitriles has been developed via a C(sp3)-H functionalization process. By employing the NiI2 and Ag2CO3 catalytic systems, the method features broad substrate scope with respect to carboxylic acids, including linear alkyl acids, cyclic acids, aryl acids and amino acids.

20.
iScience ; 20: 402-414, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31622881

RESUMO

Analysis of kidney disease-causing genes and pathology resulting from systemic diseases highlight the importance of the kidney's filtering system, the renal corpuscles. To elucidate the developmental processes that establish the renal corpuscle, we performed single-nucleus droplet-based sequencing of the human fetal kidney. This enabled the identification of nephron, interstitial, and vascular cell types that together generate the renal corpuscles. Trajectory analysis identified transient developmental gene expression, predicting precursors or mature podocytes express FBLN2, BMP4, or NTN4, in conjunction with recruitment, differentiation, and modeling of vascular and mesangial cell types into a functional filter. In vitro studies provide evidence that these factors exhibit angiogenic or mesangial recruiting and inductive properties consistent with a key organizing role for podocyte precursors in kidney development. Together these studies define a spatiotemporal developmental program for the primary filtration unit of the human kidney and provide novel insights into cell interactions regulating co-assembly of constituent cell types.

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