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1.
Food Chem ; 404(Pt A): 134382, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36252371

RESUMO

Fuzhuan brick tea (FBT) is a post-fermented dark tea preferred by consumers for its excellent hypolipidemic activity, and theabrownin (TB) is the main bioactive composition in FBT. This work explored the structural and hypolipidemic properties of TB derived from Pingwu FBT, and investigated whether it exerted hypolipidemic activity by inhibiting intestinal lipid absorption. Structural characterization revealed that TB was an amorphous polymerized phenolic compound rich in hydroxyl and carboxyl groups with good thermostability. In vivo, TB and its fractions with different molecular weights (TB-LT3k, TB-3-10k, TB-10-30k, TB-30-100k, TB-GT100k) significantly reduced the lipid levels of hyperlipidemia zebrafish (P < 0.05). Moreover, TB attenuated hyperlipidemia by inhibiting intestinal lipid absorption, as TB effectively bound to bile acids, inhibited enzymatic activity of pancreatic lipase and cholesterol esterase, influenced micelle formation, and decreased micellar cholesterol solubility. Results facilitated research on TB and offered support for its feasibility as a natural alternative to prevent hyperlipidemia.


Assuntos
Hiperlipidemias , Doenças Metabólicas , Animais , Chá/química , Peixe-Zebra , Hiperlipidemias/tratamento farmacológico , Digestão , Lipídeos
2.
Dig Dis ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36349754

RESUMO

BACKGROUND: As living standards improve, more and more people are becoming overweight or obese, which has led to a gradual increase in the incidence of gastroesophageal reflux disease (GERD). Esophageal multi-channel intraluminal impedance pH monitoring is a technique widely used in the clinical diagnosis of GERD. Commonly used traditional impedance parameters include acid exposure time (AET), number of reflux episodes (NRE), symptom association probability (SAP), and symptom index (SI). Recently, mean nocturnal baseline impedance (MNBI) and post reflux swallow-induced peristaltic wave (PSPW) index have been proposed as novel impedance parameters to evaluate esophageal mucosal integrity and esophageal chemical clearance, respectively. It was also pointed out that MNBI and PSPW index can improve the diagnostic value of impedance pH monitoring and predict the treatment outcome of GERD. SUMMARY: MNBI and PSPW index are objective and reliable novel impedance parameters with high applicability and reproducibility, which are able to improve diagnostic yield of impedance pH monitoring and predict treatment outcomes in GERD patients. KEY MESSAGES: In this paper, we mainly review the research progress of the MNBI and the PSPW index in the diagnosis and treatment of GERD.

3.
Foods ; 11(22)2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36429185

RESUMO

Bacillus species play an important role in improving the quality of some fermented foods and are also one of the dominant bacteria in Yibin Yacai (fermented mustard). However, little is known about their effects on the quality of Yibin Yacai. Here, the effect of Bacillus marcorestinctum YC-1 on the quality and microbial communities of Yibin Yacai during the fermentation process was investigated. Results indicated that the inoculation of Bacillus marcorestinctum YC-1 promoted the growth of Weissella spp. and Lactobacillus spp. and inhibited the growth of pathogens, accelerating the synthesis of free amino acids and organic acids and the degradation of nitrite. Furthermore, inoculating Yibin Yacai with YC-1 could effectively enhance the synthesis of alcohols and terpenoids in yeasts, thus producing more linalool, terpinen-4-ol, and α-muurolen in Yibin Yacai, and endowing it with pleasant floral, fruity, woody, and spicy aromas. These findings reveal that the inoculation of B. marcorestinctum YC-1 can improve the quality and safety of Yibin Yacai by changing microbial communities as fermentation proceeds.

4.
Contrast Media Mol Imaging ; 2022: 6000160, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36237578

RESUMO

Objective: To examine the psychological, compliance, and prognostic impact of care based on the Ottawa Decision Support Framework on patients with chronic heart failure. Methods: The medical profiles of 80 individuals with permanent heart failure from January 2020 to January 2021 were retrospectively analyzed. The care was provided in the framework of Ottawa Decision Support alongside the clinical standard of care. The self-assessment anxiety scale (SAS), Frankel treatment adherence scale, Minnesota quality of life questionnaire for heart failure, self-care competence scale (ESCA), complication rates, and readmission rates were compared prior to and postcare. Results: Following three-month nursing care, the score of the SAS scale was remarkably fewer, and the difference was statistically significant (P < 0.05). Three months of care later, the scores on the Frank Scale were substantially superior to those before care, and the difference was statistically significant (P < 0.05). Aftercare, the Minnesota quality of life questionnaire for heart failure was clearly inferior to precare, and the difference was statistically significant (P < 0.05). The ESCA scale scores were found to be considerably more favorable after three months of care than before care, and the difference was statistically significant (P < 0.05). After nursing care, one arrhythmia (1.25%) and one pulmonary infection (1.25%) were noted, and the difference was statistically significant (P < 0.05). The prevalence of complications was 2.50%, and the difference was statistically significant (P < 0.05). Three cases were readmitted for recurrent chronic heart failure, which was a 2.75% readmission rate. Conclusion: Continuous nursing based on the Ottawa Decision Support Framework has significant application value in an anxiety state, treatment compliance, and prognosis of patients with chronic heart failure, which can more effectively reduce patients' anxiety and the incidence of complications and readmission rate, in the meanwhile, effectively improve treatment compliance, quality of life, self-care ability, and prognosis, which is worthy of clinical application.


Assuntos
Insuficiência Cardíaca , Qualidade de Vida , Doença Crônica , Insuficiência Cardíaca/epidemiologia , Insuficiência Cardíaca/terapia , Humanos , Qualidade de Vida/psicologia , Estudos Retrospectivos
5.
Animals (Basel) ; 12(17)2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36078001

RESUMO

Garlic powder (GP) has the outstanding antibacterial, antifungal, antiviral, anti-parasitic and antioxidant characteristics because of its various contained bioactive components, such as alliin, allicin, and polysaccharide, etc. It has been widely used as a native medicine and shown to prevent a variety of diseases. This research was performed to determine the positive effects of GP on growth and intestinal function in lipopolysaccharide (LPS) challenged broilers. A total of 480 one-day-old male Ross 308 broilers of similar initial body weight were randomly divided into four groups with 8 replicates per treatment and 15 chicks each replicate. LPS challenge enhanced the weight loss rate, decreased the immunity and antioxidant capability, increased the intestinal permeability in broilers. When compared with LPS group, broilers fed with GP exhibited improved weight loss rate and jejunum villus height, enhanced ileum antioxidant function, and ameliorated intestinal barrier function. The LPS-challenged broilers in GP group had higher immunity than that of broilers in antibiotics group. GP supplementation could act as a natural alternative to antibiotic additive to alleviate the LPS-induced weight loss rate, inflammatory responses, and oxidative stress in broilers by improving the immunity and intestinal function.

6.
Comput Math Methods Med ; 2022: 2173412, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072773

RESUMO

Objective: Spread through air space (STAS) is an invasive characterization of lung adenocarcinoma and is regarded as a risk factor for poor prognosis. The aim of this study is to develop a random forest model for preoperative prediction of spread through air spaces (STAS) in stage IA lung adenocarcinoma. Methods: 92 patients with stage IA lung adenocarcinoma, who underwent computed tomography (CT) scan and surgical resection, were retrospectively reviewed. Each pulmonary nodule was automatically segmented by artificial intelligence (AI) software, and its CT-based radiomics were extracted. All patients were pathologically classified into STAS-negative and STAS-positive cohorts; then, clinical pathological and CT-based radiomics were compared between the two cohorts. Finally, a prediction model for evaluating STAS status in stage IA lung adenocarcinoma was established by a random forest model. Results: Among 92 patients with stage IA lung adenocarcinoma, STAS positive was identified in 19 patients. The random forest classification model identified predictive features, including CT maximum value, consolidation to tumor ratio (CTR), 3D diameter, CT mean value, entropy, and CT minimum value. The misclassification rate of the random forest model is only 7.69%. Conclusion: The risk factors of STAS in stage IA lung adenocarcinoma can be effectively identified based on the random forest model, and the hierarchical management of characteristic risk can be effectively realized. A random forest model for predicting STAS in IA lung adenocarcinoma is simple and practical.


Assuntos
Adenocarcinoma de Pulmão , Neoplasias Pulmonares , Adenocarcinoma de Pulmão/diagnóstico por imagem , Adenocarcinoma de Pulmão/patologia , Inteligência Artificial , Humanos , Imidazóis , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Invasividade Neoplásica/patologia , Estadiamento de Neoplasias , Estudos Retrospectivos , Software , Tomografia Computadorizada por Raios X
7.
Comput Intell Neurosci ; 2022: 4563491, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36124116

RESUMO

This study constructs a cloud computing-based college English multimedia test question modeling and application through an in-depth study of cloud computing and college English multimedia test questions. The emergence of cloud computing technology undoubtedly provides a new and ideal method to solve test data and paper management problems. This study analyzes the advantages of the Hadoop computing platform and MapReduce computing model and builds a distributed computing platform based on Hadoop using universities' existing hardware and software resources. The study analyzes the advantages of the Hadoop computing platform and the MapReduce computing model. The UML model of the system is given, the system is implemented, the system is tested functionally, and the results of the analysis are given. Multimedia is the critical link to realizing the optimization of English test questions. The proper use of multimedia test questions will undoubtedly become an inevitable trend in the development of English test questions in the future, which requires every worker on the education front to continuously analyze and study the problems arising from multimedia teaching, summarize the experience of multimedia teaching, and explore new methods of multimedia teaching, so that multimedia teaching can better promote the optimization of English test questions in colleges and universities and better serve the education teaching.


Assuntos
Computação em Nuvem , Multimídia , Computadores , Humanos , Software , Universidades
8.
Comput Math Methods Med ; 2022: 5787808, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118827

RESUMO

miRNA and m6A methylation are two key regulators in cancers. However, in acute myeloid leukemia (AML), the relationship of miRNA and m6A methylation remains unclear. The present work is aimed at determining the effect of m6A methylation induced by miRNAs on AML and its underlying mechanism. The expression of METTL14 was detected by qRT-PCR and western blot. The growth of HL-60 cells was analyzed by CCK-8, Transwell assay, and flow cytometry. Tumor-bearing mice were established, and Ki-67 staining assay was used to detect the proliferation in vivo. Dual luciferase reporter system detected the effect of miR-1306-5p on METTL14 luciferase activity. Dot blot analysis detected m6A methylation. We found that METTL14 was upregulated in AML patients and overexpressed METTL14 promoted AML development. Further analysis indicated that METTL14 was directly targeted by miR-1306-5p and overexpressed miR-1306-5p alleviated AML progression. In addition, m6A methylation level regulated by METTL14 could be affected by miR-1306-5p. In conclusion, we found that suppressed miR-1306-5p enhanced AML progression by elevating m6A methylation level via upregulating METTL14. These findings provided basis for the development of new strategies for treating AML.


Assuntos
Leucemia Mieloide Aguda , Metiltransferases/metabolismo , MicroRNAs/metabolismo , Animais , Linhagem Celular Tumoral , Proliferação de Células , Antígeno Ki-67/metabolismo , Leucemia Mieloide Aguda/genética , Metilação , Camundongos , MicroRNAs/genética
9.
Chemistry ; : e202202680, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36170107

RESUMO

Organelle-targeted type I photodynamic therapy (PDT) shows great potential to overcome the hypoxic microenvironment in solid tumors. The endoplasmic reticulum (ER) is an indispensable organelle in cells with important biological functions. When the ER is damaged due to the production of reactive oxygen species (ROS), the accumulation of misfolded proteins will interfere with ER homeostasis, resulting in ER stress. Here, an ER-targeted benzophenothiazine-based photosensitizer NBS-ER was presented. ER targeting modification significantly reduced the dark toxicity and improved phototoxicity index (PI). NBS-ER could effectively produce O2 - ⋅ with near-infrared irradiation, making its phototoxicity under hypoxia close to that under normoxia. Meanwhile, the photoinduced ROS triggered ER stress and induced apoptosis. In addition, NBS-ER possessed excellent photodynamic therapeutic effect in 4T1-tumor-bearing mice.

10.
ACS Omega ; 7(33): 29086-29099, 2022 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-36033659

RESUMO

Helicobacter pylori infection is a leading cause of gastritis and peptic ulcer. Current treatments for H. pylori are limited by the increase in antibiotic-resistant strains and low drug delivery to the infection site, indicating the need for effective delivery systems of antibiotics. Although liposomes are the most successful drug delivery carriers that have already been applied commercially, their acidic stability still stands as a problem. Herein, we developed a novel nanoliposome using cosmetic raw materials of mannosylerythritol lipid-B (MEL-B), soy bean lecithin, and cholesterol, namely, LipoSC-MELB. LipoSC-MELB exhibited enhanced stability under the simulated gastric-acid condition, owing to its strong intermolecular hydrogen-bond interactions caused by the incorporation of MEL-B. Moreover, amoxicillin-loaded LipoSC-MELB (LipoSC-MELB/AMX) had a particle size of approximately 100 nm and exhibited sustained drug release under varying pH conditions (pH 3-7). Besides, LipoSC-MELB/AMX exhibited significantly higher anti-H. pylori and anti-H. pylori biofilm activity as compared with free AMX. Furthermore, LipoSC-MELB was able to carry AMX across the barriers of gastric mucus and H. pylori biofilms. Remarkably, in vivo assays indicated that LipoSC-MELB/AMX was effective in treating H. pylori infection and its associated gastritis and gastric ulcers. Overall, the findings of this study showed that LipoSC-MELB was effective for gastromucosal delivery of amoxicillin to improve its bioavailability for the treatment of H. pylori infection.

11.
Redox Biol ; 56: 102437, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36037588

RESUMO

Maternal stress has been associated with poor birth outcomes, including preterm birth, infant mortality, and low birth weight. Bone development disorders in the embryo as a result of maternal stress are believed to be mediated through oxidative stress damage. Various species of free radicals, such as alkoxyl radicals, can be formed through endogenous redox response or exogenous stimuli in the womb and transmitted to embryos. Yet, whether these free radicals lead to abnormal fetal bone development is unclear. Here, we demonstrate prenatal bone growth retardation and ferroptosis-related signals of chondrocytes were induced by classic alkoxyl radical generators. We also show that alkoxyl radicals lead to significant accumulation of oxidized phospholipids in chondrocytes, through the iron-mediated Fenton reaction in embryos. We further demonstrate a role for the lipid peroxidation end product, 4-HNE, which forms adducts with the pivotal chondrogenesis transcription factor SOX9, leading to its degradation, therefore dampening chondrogenesis. Our data define a critical role for phospholipid peroxidation in alkoxyl radicals-evoked abnormal chondrogenesis, and pinpoint it being a precise target for treating oxidative stress-related bone development disorders.


Assuntos
Fosfolipídeos , Nascimento Prematuro , Álcoois , Desenvolvimento Ósseo , Condrogênese , Feminino , Radicais Livres/metabolismo , Humanos , Recém-Nascido , Ferro , Peroxidação de Lipídeos , Fosfolipídeos/metabolismo , Fatores de Transcrição/metabolismo
12.
Front Nutr ; 9: 946096, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35967771

RESUMO

Postbiotics are the inactive bacteria and/or metabolites of beneficial microbes which have been recently found to be as effective as their live probiotic. This study aimed to evaluate the benefits of Lactobacillus plantarum (LP)-derived postbiotics on ameliorating Salmonella-induced neurological dysfunctions. Mice were pretreated with LP postbiotics (heat-killed bacteria or the metabolites) or active bacteria, and then challenged with Salmonella enterica Typhimurium (ST). Results showed that LP postbiotics, particularly the metabolites, effectively prevented ST infection in mice, as evidenced by the inhibited weight loss, bacterial translocation, and tissue damages. The LP postbiotics markedly suppressed brain injuries and neuroinflammation (the decreased interleukin (IL)-1ß and IL-6, and the increased IL-4 and IL-10). Behavior tests indicated that LP postbiotics, especially the metabolites, protected mice from ST-induced anxiety and depressive-like behaviors and cognitive impairment. A significant modulation of neuroactive molecules (5-hydroxytryptamine, gamma-aminobutyric acid, brain-derived neurotrophic factor, dopamine, acetylcholine, and neuropeptide Y) was also found by LP postbiotic pretreatment. Microbiome analysis revealed that LP postbiotics optimized the cecal microbial composition by increasing Helicobacter, Lactobacillus and Dubosiella, and decreasing Mucispirillum, norank_f_Oscillospiraceae, and Eubacterium_siraeum_group. Moreover, LP postbiotics inhibited the reduction of short-chain fatty acids caused by ST infection. Pearson's correlation assays further confirmed the strong relationship of LP postbiotics-mediated benefits and gut microbiota. This study highlights the effectiveness of postbiotics and provide a promising strategy for preventing infection-induced brain disorders by targeting gut-brain axis.

13.
ACS Appl Mater Interfaces ; 14(31): 36105-36115, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35899424

RESUMO

Polysiloxane/molybdenum disulfide (MoS2) composite lubricating coatings that are used in low-earth orbit spacecraft have high resistance to atomic oxygen attack and outstanding vacuum tribological properties. However, their atmospheric environment wear resistance and adaptability under high humidity are poor. In this paper, octa-aminophenyl T8-polyhedral oligomeric silsesquioxane (NH2-POSS) was used to enhance their atmospheric wear resistance and environmental adaptability to increase the protection of polysiloxane lubricating coatings for spacecraft. In this study, the effect of NH2-POSS on the mechanical properties of a polysiloxane coating and the tribological properties of the POSS-enhanced polysiloxane/MoS2 lubricating coating were investigated and the mechanism of NH2-POSS enhancement was discussed. Results showed that the addition of NH2-POSS markedly improved the hardness and elastic modulus of the polysiloxane coatings by increasing the crosslink density of the polysiloxanes and nanofiller effect of POSS. Among them, 6 wt% NH2-POSS can reduce the wear rate of the lubricating coating by 25%. In addition, this study also explores the adaptability of polysiloxane/MoS2 lubricating coatings in a high-humidity environment and an atomic oxygen irradiation environment, which provides a reference for further optimization of polysiloxane lubricating coatings.

14.
Biomaterials ; 287: 121665, 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35809403

RESUMO

The complexity of existing methods for biodegradation control limits the multi-functionality of biomedical materials. It is urgent to develop simple and straightforward strategies to control the biodegradation rate with precise tracking of various parameters in real-time. Here, we show an imaging moiety-directed co-assembly strategy, in which different imaging moieties bearing non-covalent interaction sites are covalently introduced into the poly (D,l-lactic acid) (PDLLA) chain as end groups, followed by alternate non-covalent interactions with polymer chains upon compression molding. This strategy takes advantage of a variety of bonding types (including CH-π, CH-F, etc.) to firmly integrate the PDLLA chains and strongly control the biodegradation rate, making the amorphous prototype degraded much slower than higher-molecular-weight counterparts, and the local inflammatory response is insignificant. On this basis, a synchronous four-modal (X-ray computed tomography + fluorescence + photoacoustics + ultrasound) imaging was achieved on the single entity in vivo, even within a millimeter-scale thick-skin tissue. These imaging signals can precisely correlate the multi parameter variation trend of material mass, volume and molecular weight, signifying that co-assembly can be utilized to develop advanced theranostic systems. SINGLE SENTENCE SUMMARY: We developed an imaging moiety-directed co-assembly strategy to control the biodegradation rate and achieve the synchronization of real-time four-modal imaging in vivo. These imaging signals can precisely correlate the multi-parameter variation trend of material mass, volume and molecular weight, which provided comprehensive biomedical information accessing both qualitatively and quantitatively.

15.
Comput Intell Neurosci ; 2022: 5239375, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35845915

RESUMO

This paper analyzes and investigates the quality assessment of spoken English pronunciation using a cognitive heuristic computing approach and designs a corresponding spoken pronunciation quality assessment system for practical training. Using the general Goodness of Pronunciation assessment algorithm as a benchmark, the shortcomings of the traditional Goodness of Pronunciation method are explored through statistical experiments, and the validity of the overall posterior probability output from the speech model for pronunciation quality assessment is verified. For the analysis of rhythm, there is no common algorithm framework, but in this paper, the F0 similarity algorithm based on dynamic time regularization and the stop similarity algorithm based on forced alignment is proposed for the two main factors of rhythm, intonation, and pause, respectively. After framing, the Hamming window processing is used to make the signal smoother, reduce the side lobe size after fast Fourier transform processing, and solve the problem of spectrum leakage. Compared with the ordinary rectangular window function, the Hamming window can obtain a higher quality spectrum. And combined with CTC for speech recognition modeling, the recognition rates are comparable in the case of using BLSTM and bidirectional threshold cyclic unit BGRU as the hidden layer unit, respectively, and the training time is 23% less than BLSTM using BGRU; in addition, the BGRU-CTC model is improved by using a 2-BGRU-CTC model with 256 hidden layer nodes, so that the error rate of phoneme recognition is reduced to 33%. The effectiveness of the algorithm framework is also verified through experiments, which further proves the effectiveness of our proposed phoneme segment feature and rhyme similarity algorithm.


Assuntos
Heurística , Fonética , Cognição , Idioma , Fala
16.
Animals (Basel) ; 12(13)2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35804509

RESUMO

Bacillus licheniformis (B. licheniformis) is a safe probiotic that can promote animal growth and inhibit pathogenic bacteria. This study aimed to assess the effects of B. licheniformis, one green feed additive, on growth performance, diarrhea incidence, immune function, fecal volatile fatty acids, and microflora structure in weaned piglets. Weaned piglets (n = 180) were randomly divided into three treatment groups and fed a basal diet and a basal diet supplemented with 500 mg B. licheniformis per kg and 1000 mg B. licheniformis per kg, respectively. The dietary 500 mg/kg B. licheniformis inclusion improved the average daily gain, reduced diarrhea incidence, and strengthened antioxidant capacity. Piglets supplemented with B. licheniformis presented increased serum immunoglobulins (IgA, IgM) compared to the CON group. Meanwhile, the expression of anti-inflammation factors was increased, and the levels of pro-inflammation factors were reduced after B. licheniformis administration. Moreover, the levels of volatile fatty acids, including acetic acid, propionic acid, butyric acid, isobutyric acid, and isovaleric acid, in the BL500 and BL1000 groups were increased compared with the CON group, and the concentration of valeric acid was higher in the BL500 group. Furthermore, piglets in the 500 mg/kg B. licheniformis addition group significantly altered fecal microbiota by increasing Clostridium_sensu_stricto_1 and Oscillospira. In conclusion, dietary B. licheniformis relieved diarrhea, enhanced antioxidant capacity, immunity function, and fecal microflora structure in weaned pigs.

17.
Acta Pharm Sin B ; 12(4): 1913-1927, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35847518

RESUMO

Mutations in the plant homeodomain-like finger protein 6 (PHF6) gene are strongly associated with acute myeloid (AML) and T-cell acute lymphoblastic leukemia (T-ALL). In this study, we demonstrated that PHF6 can bind to H3K9me3 and H3K27me1 on the nucleolar chromatin and recruit histone methyltransferase SUV39H1 to the rDNA locus. The deletion of PHF6 caused a decrease in the recruitment of SUV39H1 to rDNA gene loci, resulting in a reduction in the level of H3K9me3 and the promotion of rDNA transcription. The knockdown of either SUV39H1 or PHF6 significantly attenuated the effects of increase in H3K9me3 and suppressed the transcription of rDNA induced by the overexpression of the other interacting partner, thereby establishing an interdependent relationship between PHF6 and SUV39H1 in their control of rRNA transcription. The PHF6 clinical mutants significantly impaired the ability to bind and recruit SUV39H1 to the rDNA loci, resulting in an increase in rDNA transcription activity, the proliferation of in vitro leukemia cells, and the growth of in vivo mouse xenografts. Importantly, significantly elevated levels of pre-rRNA were observed in clinical AML patients who possessed a mutated version of PHF6. The specific rDNA transcription inhibitor CX5461 significantly reduced the resistance of U937 AML cells deficient in PHF6 to cytarabine, the drug that is most commonly used to treat AML. Collectively, we revealed a novel molecular mechanism by which PHF6 recruits methyltransferase SUV39H1 to the nucleolar region in leukemia and provided a potential therapeutic target for PHF6-mutant leukemia.

18.
Chem Sci ; 13(18): 5085-5106, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35655575

RESUMO

Photodynamic therapy (PDT) utilizes light-activated photosensitizers (PSs) to generate toxic species for therapeutics. It has become an emerging solution for cancer treatment because of its specific spatiotemporal selectivity and minimal invasiveness. Noble metal (Ru, Ir and Pt) complexes are of increasing interest as photosensitizers for their excellent photophysical, photochemical, and photobiological properties. In this review, we highlight recent advancements in the development of noble metal complex photosensitizers for PDT during the last 5 years. We will summarize the design strategies of noble metal complexes for efficient and precise PDT, including increasing the light penetration depth, reducing the oxygen-dependent nature and improving target ability. Finally, we summarize recent efforts for the development of noble-based PSs and discuss the limitations of such PSs in clinical application and future perspectives in this field, such as the combination of PDT with other treatment modalities.

19.
Foods ; 11(11)2022 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-35681332

RESUMO

Fixation is a key process contributing to different flavors of green tea and pan-fire and steam were the common fixation methods applied conventionally. In this study, pan-fired green tea (PGT) and steamed green tea (SGT) produced by different fixation methods were compared in characteristic biochemicals including volatile compounds, amino acids, catechins and alkaloids, together with evaluating their tastes and lipid-lowering effects. PGT and SGT could be distinguished clearly by orthogonal partial least squares discriminant analysis (OPLS-DA) and heatmap. SGT had higher contents of volatile alcohols (44.75%) with green and floral attributes, while PGT had higher contents of volatile esters (22.63%) with fruity and sweet attributes. Results of electronic tongue analysis showed that PGT and SGT had similar taste of strong umami and sweetness, but little astringency and bitterness. In addition, amino acids were more abundant in PGT (41.47 mg/g in PGT, 33.79 mg/g in SGT), and catechins were more abundant in SGT (111.36 mg/g in PGT, 139.68 mg/mg in SGT). Zebrafish larvae high-fat model was applied to study the lipid-lowering effects of PGT and SGT. Results showed that both SGT and PGT had lipid-lowering effects, and the lipid level was decreased to 61.11 and 54.47% at concentration of 300 mg/L compared to high-fat group, respectively. Generally, different fixation methods of pan-fire and steam showed significant effects on aroma and contents of characteristic chemical compounds (amino acids and catechins) of green tea, but no effects on the taste and lipid-lowering activity.

20.
Front Nutr ; 9: 886256, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35571898

RESUMO

Objectives: Gut microbes influence lipid metabolism and immune responses that are key features of metabolic disorders. This study examined effects of bacterial rhamnolipids (RLS) on lipid metabolism, immune response, and gut microbiota in rats. Methods: Twenty-four Sprague-Dawley rats were randomly divided into three groups and gavage-fed for seven weeks with normal saline (NCO group), 50 mg/kg bw RLS (RLS1 group), and 100 mg/kg bw RLS (RLS2 group). Results: Compared with those of the NCO group, the RLS1 and RLS2 groups showed significantly decreased fat weight, relative fat weight, and adipocyte size (P < 0.05). Furthermore, RLS1 and RLS2 significantly decreased concentrations of triglycerides, low-density lipoprotein-cholesterol, and non-esterified fatty acids and increased high-density lipoprotein-cholesterol levels (P < 0.05). However, the total cholesterol content among the three groups (P > 0.05) were not significantly different. Serum concentrations of interleukin-1ß, interleukin-6, and tumor necrosis factor-α were significantly lower in the RLS2 group than those in the NCO group (P < 0.05). The relative mRNA expression of fatty acid synthase was significantly decreased, while those of carnitine palmitoyltransferase-1, carnitine palmitoyltransferase-2, and peroxisome proliferator-activated receptor-gamma coactivator-1α were significantly increased in the RLS2 group compared with those in the NCO group (P < 0.05). Moreover, the relative abundances of Lactobacillus, Roseburia, Ruminococcus-1, and Parabacteroides were significantly higher in the RLS2 group than those in the NCO group (P < 0.05). Conclusion: Our findings suggest that RLS reduces fat deposition, inhibits inflammation, regulates intestinal flora, and promotes the proliferation of beneficial bacteria in rats.

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