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1.
Food Funct ; 12(19): 9416-9431, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34606558

RESUMO

Sonneratia apetala seeds are considered as prospective nutraceuticals with a high content of unsaturated fatty acids (UFAs) which are mainly distributed in the oil. It is well-known that UFAs could exhibit urate-lowering potency and protect against renal injury, indicating that S. apetala seed oil (SSO) may possess hypouricemic and nephroprotective effects. Consequently, the present work attempted to probe into the effects and mechanisms of SSO on potassium oxonate/hypoxanthine-induced hyperuricemia and associated renal injury. The results indicated that SSO treatment prominently inhibited the increase of serum uric acid (UA), creatinine (CRE), and urea nitrogen (BUN) levels and hepatic xanthine oxidase (XOD) activity in hyperuricemia mice. Kidney indexes and histopathological lesions were also remarkably ameliorated. Additionally, SSO treatment improved the renal anti-oxidant status in hyperuricemia mice by significantly reversing the increase in ROS and MDA levels as well as the decline in SOD, CAT and GSH-Px activities. SSO dramatically downregulated the expression and secretion of pro-inflammatory factors involving MCP-1, IL-1ß, IL-6, IL-18 and TNF-α elicited by hyperuricemia. Furthermore, after SSO treatment, increased protein expressions of GLUT9, URAT1 and OAT1 in the hyperuricemia mice were obviously reversed. SSO treatment enormously restored Nrf2 activation and subsequent translation of related anti-oxidative enzymes in the kidneys. TXNIP/NLRP3 inflammasome activation was also obviously suppressed by SSO. In conclusion, SSO exerted favorable hypouricemic effects owing to its dual functions of downregulating the XOD activity and modulating the expressions of renal urate transport-associated proteins, and it also could alleviate hyperuricemia-induced renal injury by restoring the Keap1-Nrf2 pathway and blocking the TXNIP/NLRP3 inflammasome activation.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34546926

RESUMO

The use of modular mining methods to divide genes into multiple gene subsets is currently the basic work of analyzing biological big data and exploring the biological interpretation of it. By transforming gene expression profile data into gene co-expression network, we transform the task of gene module detection into the problem of finding community structure in the graph, and introduce the latest network representation learning method-graph neural network to optimize this problem. In order to systematically evaluate whether the algorithm allows overlap to affect such problems, we make two variants of the output of the algorithm, Deepgmd_cluster and Deepgmd, the difference between them is whether overlap is allowed. By comparing the known modules and the modules generated by the algorithm, we can evaluate the quality of the algorithm. We use this method to compare our algorithm with some current mainstream methods. The results show that our method has greater advantages. In the end, we analyze some typical modules from the modules found by the algorithm for visualization, and use the GO database and KEGG database to perform enrichment analysis and pathway analysis on these modules.

3.
Front Pharmacol ; 12: 698219, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34483901

RESUMO

Sonneratia apetala Buch-Ham., an exotic mangrove species with antidiabetic, antibacterial, and antioxidant capacities, mainly distributes in the southeast coastal areas in China. The present work investigated the protective effects of Sonneratia apetala leaves and branches extraction (SAL) on hyperuricemia (HUA) in mice. Potassium oxonate (PO) and hypoxanthine (HX) were used to establish the HUA model by challenge for consecutive 7 days. Results revealed that SAL inhibited the increases in kidney weight and index compared to the vehicle group. Meanwhile, SAL significantly decreased the levels of uric acid (UA), creatinine (CRE), and blood urea nitrogen (BUN) in serum. Additionally, SAL inhibited the activity of xanthine oxidase (XOD) in the liver. SAL ameliorated PO- and HX-induced histopathological changes. Moreover, it regulated oxidative stress markers including malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) activity, and glutathione (GSH) content. Also, SAL inhibited the increases in renal levels of interleukin-6 (IL-6), interleukin-18 (IL-18), interleukin-1ß (IL-1ß), tumor necrosis factor (TNF-α), monocyte chemotactic protein 1 (MCP-1), and transforming growth factor-ß (TGF-ß). SAL remarkably reduced suppressor of cytokine signaling 3 (SOCS3), Janus kinase 2 (JAK2), and subsequent phosphorylation of signal transducer and activator of transcription 3 (STAT3) expression. In addition, SAL inhibited the activation of nuclear factor kappa-B (NF-κB) in the kidney. Furthermore, SAL protected against HUA by regulating renal UA transporters of organic anion transporter (OAT1), urate reabsorption transporter 1 (URAT1), and glucose transporter 9 (GLUT9). These findings suggested that SAL ameliorated HUA by inhibiting the production of uric acid and enhancing renal urate excretion, which are related to oxidative stress and inflammation, and the possible molecular mechanisms include its ability to inhibit the JAK/STAT signaling pathway. Thus, SAL might be developed into a promising agent for HUA treatments.

4.
Ann Transl Med ; 9(15): 1238, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532375

RESUMO

Background: Surgical operation plays an important role in the treatment of cancer. The success of the operation lies in the complete removal of the primary and disseminated tumor tissue while preserving the normal tissue. The development of optical molecular image navigation technology has provided a new option for intraoperative tumor visualization. In this study, a fluorescence imaging navigation system was used to detect the diameter of mice tumors and provide experimental evidence for the further development of digital diagnosis and treatment equipment. Methods: The minimum detection concentration in vitro of the fluorescence imaging navigation system for indocyanine green (ICG) was first detected, then 120 female Institute of Cancer Research (ICR) mice and 120 female BALB/c nude mice were randomly divided into three groups by weight, high-dose (H, 4 mg/kg), middle-dose (M, 2 mg/kg), and low-dose (L, 1 mg/kg) groups of ICG solution. After inoculating solid tumors, high, medium, and low doses of ICG were injected via the tail vein, and the tumor diameter was measured by a fluorescence imaging navigation system and vernier caliper within 24 hours of injection. Results: The minimum detectable diameter of the system could reach 0.2 mm compared with the vernier caliper, and the actual measurement error was within 0.2 mm. Conclusions: A fluorescence imaging navigation system has high accuracy and sensitivity in the application of tumor detection, which may assist the clinical diagnosis and treatment of tumors.

5.
Chem Commun (Camb) ; 57(71): 8977-8980, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34486619

RESUMO

This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.


Assuntos
Proteínas de Bactérias/química , Técnicas Biossensoriais/métodos , Proteínas Associadas a CRISPR/química , Sistemas CRISPR-Cas , DNA Viral/análise , Endodesoxirribonucleases/química , DNA de Cadeia Simples/química , Dopamina/química , Técnicas Eletroquímicas/métodos , Fluoresceínas/química , Corantes Fluorescentes/química , Papillomavirus Humano 16/química , Lipossomos/química , Fenômenos Magnéticos , Processos Fotoquímicos
6.
Arch Anim Breed ; 64(2): 405-416, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34584942

RESUMO

Skeletal muscle growth and development from embryo to adult consists of a series of carefully regulated changes in gene expression. This study aimed to identify candidate genes involved in chicken growth and development and to investigate the potential regulatory mechanisms of early growth in Haiyang yellow chicken. RNA sequencing was used to compare the transcriptomes of chicken muscle tissues at four developmental stages. In total, 6150 differentially expressed genes (DEGs) ( | fold change |   ≥  2; false discovery rate (FDR)  ≤  0.05) were detected by pairwise comparison in female chickens. Functional analysis showed that the DEGs were mainly involved in the processes of muscle growth and development and cell differentiation. Many of the DEGs, such as MSTN, MYOD1, MYF6, MYF5, and IGF1, were related to chicken growth and development. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the DEGs were significantly enriched in four pathways related to growth and development: extracellular matrix (ECM)-receptor interaction, focal adhesion, tight junction, and insulin signalling pathways. A total of 42 DEGs assigned to these pathways are potential candidate genes for inducing the differences in growth among the four development stages, such as MYH1A, EGF, MYLK2, MYLK4, and LAMB3. This study identified a range of genes and several pathways that may be involved in regulating early growth.

7.
Int J Mol Sci ; 22(15)2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34360755

RESUMO

Increasing attention is being focused on the use of polypeptide-based N-methyl-d-aspartate (NMDA) receptor antagonists for the treatment of nervous system disorders. In our study on Achyranthes bidentata Blume, we identified an NMDA receptor subtype 2B (NR2B) antagonist that exerts distinct neuroprotective actions. This antagonist is a 33 amino acid peptide, named bidentatide, which contains three disulfide bridges that form a cysteine knot motif. We determined the neuroactive potential of bidentatide by evaluating its in vitro effects against NMDA-mediated excitotoxicity. The results showed that pretreating primary cultured hippocampal neurons with bidentatide prevented NMDA-induced cell death and apoptosis via multiple mechanisms that involved intracellular Ca2+ inhibition, NMDA current inhibition, and apoptosis-related protein expression regulation. These mechanisms were all dependent on bidentatide-induced inhibitory regulation of NR2B-containing NMDA receptors; thus, bidentatide may contribute to the development of neuroprotective agents that would likely possess the high selectivity and safety profiles inherent in peptide drugs.


Assuntos
Achyranthes/química , Hipocampo/metabolismo , Neurônios/metabolismo , Fármacos Neuroprotetores , Peptídeos , Proteínas de Plantas , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Animais , Apoptose/efeitos dos fármacos , Cálcio/metabolismo , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Fármacos Neuroprotetores/farmacologia , Peptídeos/química , Peptídeos/isolamento & purificação , Peptídeos/farmacologia , Proteínas de Plantas/química , Proteínas de Plantas/isolamento & purificação , Proteínas de Plantas/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores de N-Metil-D-Aspartato/metabolismo
8.
Sensors (Basel) ; 21(16)2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34451082

RESUMO

This study concerns the influence of chemical corrosion and geometric parameters on the macroscopic damage characteristics of brittle limestone containing a kinked fissure under uniaxial compression. The specimens are prepared in chemical solutions with different NaCl concentrations and pH values. The acoustic emission (AE) technique is adopted to detect the inner distortion of the failure behaviour. The physical process of the crack coalescence of kinked fissures is synchronously captured by a high-speed camera. Seven failure patterns are identified based on the final failure mode and the failure process. Furthermore, the stress intensity factor of kinked cracks under chemical corrosion is obtained by a theoretical analysis. Chemical erosion with an acidic solution has a relatively strong effect on the compressive strength of the tested specimen, while the initial crack angle is not affected by short-term chemical corrosion.

9.
Ann Transl Med ; 9(14): 1167, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34430608

RESUMO

Background: To investigate whether indocyanine green (ICG) fluorescence imaging can be used to evaluate chronic and acute liver injury induced by either a high-fat (HF) diet or carbon tetrachloride (CCl4). Methods: Sprague-Dawley (SD) rats were randomly divided into three groups: control group, HF diet-induced model group, and CCl4-induced model group. The chronic and acute liver injury models were induced by a HF diet and intraperitoneal injection of CCl4, respectively. After HF feeding, the liver index, levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of the rats were determined. The livers were also collected to evaluate histopathology damage by hematoxylin and eosin (H&E) staining. After in vitro perfusion of the liver and ICG administration, the liver fluorescence intensity and corresponding spectral value were measured by using real-image guided system (REAL-IGS). Results: After HF feeding, the liver index and levels of serum ALT and AST were significantly increased, and the livers of the rats showed severe histopathological changes. Compared with the control group, the hepatic lobes of the model rats exhibited incomplete green fluorescence, and the corresponding spectral value was markedly reduced. Conclusions: ICG fluorescence imaging can be used to evaluate liver injury induced by either a HF diet or CCl4.

10.
Phys Rev Lett ; 127(2): 020602, 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34296924

RESUMO

We experimentally study the ergodic dynamics of a 1D array of 12 superconducting qubits with a transverse field, and identify the regimes of strong and weak thermalization with different initial states. We observe convergence of the local observable to its thermal expectation value in the strong-thermalizaion regime. For weak thermalization, the dynamics of local observable exhibits an oscillation around the thermal value, which can only be attained by the time average. We also demonstrate that the entanglement entropy and concurrence can characterize the regimes of strong and weak thermalization. Our work provides an essential step toward a generic understanding of thermalization in quantum systems.

11.
Arch Anim Breed ; 64(1): 45-52, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34084903

RESUMO

Anti-Müllerian hormone (AMH), a member of the transforming growth factor- ß superfamily, plays important regulatory roles in follicular development and sex differentiation. Although much has been learned about the impact of polymorphisms of AMH on reproduction in animals, the effect on chicken reproduction is not well explored. In this study, the polymorphism of five exons of AMH gene and its effect on the reproductive performance of Jinghai Yellow chickens were studied. Primers for the amplification of AMH exons were designed, and Sanger sequencing was performed. Finally, only the polymorphism in the second exon of the AMH gene was found in the present population. Polymorphisms in the second exon of the AMH gene in 246 Jinghai Yellow hens and their associations with reproductive traits were analyzed. In total, four single nucleotide polymorphism (SNP) mutations were detected in the second exon of the AMH gene: g.1868A > C (AA, aa and Aa); g.1883G > A (BB, bb and Bb); g.1987G > A (CC, cc and Cc); and g.1996A > G (DD, dd and Dd). Only the mutation of g.1996A > G affected the reproductive traits: the age of laying first egg (AFE) of dd genotype was significantly ( p < 0.01 ) earlier than that in the DD and Dd hens. Moreover, the egg number by 300 d old (EN300) of dd individuals was significantly higher than that of DD and Dd individuals ( p < 0.01 ). Thus, we inferred that the dd genotype is the beneficial genotype. Additionally, AFE and EN300 showed significantly better performance in both the H2H2 and H7H7 diplotypes compared with other diplotype individuals ( p < 0.01 ). Thus, the H2H2 and H7H7 genotype had the best combination of AFE and EN300. Our study may allow for molecular marker section in poultry breeding.

12.
Angew Chem Int Ed Engl ; 60(36): 19933-19941, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34128294

RESUMO

Fast water transport channels are crucial for water-related membrane separation processes. However, overcoming the trade-off between flux and selectivity is still a major challenge. To address this, we constructed spherical polyelectrolyte brush (SPB) structures with a highly hydrophilic polyelectrolyte brush layer, and introduced them into GO laminates, which increased both the flux and the separation factor. At 70 °C, the flux reached 5.23 kg m-2 h-1 , and the separation factor of butanol/water increased to ≈8000, which places it among the most selective separation membranes reported to date. Interestingly, further studies demonstrated that the enhancement of water transport was not only dependent on the hydrophilicity of the polyelectrolyte chains, but also influenced by their flexibility in the solvent. Quartz crystal microbalance with dissipation and molecular dynamics simulations revealed the structure-performance correlations between water molecule migration and the flexibility of the ordered polymer chains in the 2D confined space.

13.
Science ; 372(6545): 948-952, 2021 05 28.
Artigo em Inglês | MEDLINE | ID: mdl-33958483

RESUMO

Quantum walks are the quantum mechanical analog of classical random walks and an extremely powerful tool in quantum simulations, quantum search algorithms, and even for universal quantum computing. In our work, we have designed and fabricated an 8-by-8 two-dimensional square superconducting qubit array composed of 62 functional qubits. We used this device to demonstrate high-fidelity single- and two-particle quantum walks. Furthermore, with the high programmability of the quantum processor, we implemented a Mach-Zehnder interferometer where the quantum walker coherently traverses in two paths before interfering and exiting. By tuning the disorders on the evolution paths, we observed interference fringes with single and double walkers. Our work is a milestone in the field, bringing future larger-scale quantum applications closer to realization for noisy intermediate-scale quantum processors.

14.
Phys Rev Lett ; 126(9): 090502, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33750174

RESUMO

Topological quantum computation based on anyons is a promising approach to achieve fault-tolerant quantum computing. The Majorana zero modes in the Kitaev chain are an example of non-Abelian anyons where braiding operations can be used to perform quantum gates. Here we perform a quantum simulation of topological quantum computing, by teleporting a qubit encoded in the Majorana zero modes of a Kitaev chain. The quantum simulation is performed by mapping the Kitaev chain to its equivalent spin version and realizing the ground states in a superconducting quantum processor. The teleportation transfers the quantum state encoded in the spin-mapped version of the Majorana zero mode states between two Kitaev chains. The teleportation circuit is realized using only braiding operations and can be achieved despite being restricted to Clifford gates for the Ising anyons. The Majorana encoding is a quantum error detecting code for phase-flip errors, which is used to improve the average fidelity of the teleportation for six distinct states from 70.76±0.35% to 84.60±0.11%, well beyond the classical bound in either case.

15.
Opt Express ; 29(4): 6080-6091, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33726137

RESUMO

Exceptional points (EPs) could potentially enhance the sensitivity of an optical sensing system by orders of magnitude. Higher-order EP systems, having more complex physics, can further boost this parameter. In this paper, we investigate the response order of high-order non-Hermitian systems and provide a guideline for designing a sensor with high response order. Based on this design rule, we propose and demonstrate an optical sensor with a fourth-order response, and analyze its associated properties. The four resonant wavelengths of our optical sensor simultaneously collapse at a high-order exceptional point in the parameter space, providing a fourth root relation between the amount of wavelength splitting and the amplitude of the perturbation. A large sensitivity enhancement factor over 100, is observed when the wavelength splitting is compared with traditional single resonator-based sensors under small perturbation conditions.

16.
Food Funct ; 12(6): 2554-2568, 2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33625409

RESUMO

Ulcerative colitis is a recrudescent intestinal inflammation coupled with diarrhea, weight loss, pus, and blood stool, which seriously impacts the quality of patient life. d-Pinitol, which can be a food supplement isolated from the food plant-like soybeans, Ceratonia siliqua Linn and Bruguiera gymnorrhiza, has been proved to show anti-oxidative and anti-inflammatory effects. However, the potential mechanism of d-pinitol still remains ill-defined contemporarily. In the current study, the therapeutic effect and potential mechanisms of d-pinitol against colitis were investigated. Oxidative stress and inflammation of experimental colitis were caused by 3% DSS treatment once daily for 7 days. During DSS treatment, the mice of the positive drug group and three other groups were orally administered SASP or d-pinitol once daily. Clinical symptoms were analyzed, and macroscopic scores were calculated. The levels of oxidative and inflammatory cytokines were measured using assay kits and RT-PCR. Additionally, the protein expression of the Nrf2/ARE pathway and PPAR-γ was measured by Western blot. Results showed that d-pinitol enormously alleviated DSS-induced bodyweight loss, colon shortening, and histological injuries, achieving a therapeutic efficacy superior to SASP. Moreover, the oxidative stress and colonic inflammatory response were mitigated. d-pinitol not only significantly activated the Nrf2/ARE signaling pathway via facilitating the translocation of Nrf2 from sitoplazma to cytoblast, upregulating the protein expression levels of GCLC, GCLM, HO-1, and NQO1, but also improved the PPAR-γ level by binding to the active site of PPAR-γ, when suppressing NF-κB p65 and IκBα phosphorylation. In conclusion, d-pinitol exhibited a dramatic anti-colitis efficacy by activating the Nrf2/ARE pathway and PPAR-γ. Hence, d-pinitol may be a promising therapeutic drug against UC in the future.


Assuntos
Colite/metabolismo , Inositol/análogos & derivados , Fator 2 Relacionado a NF-E2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Suplementos Nutricionais , Modelos Animais de Doenças , Inositol/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/metabolismo , PPAR gama/metabolismo
17.
Pain Manag Nurs ; 2021 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-33579615

RESUMO

BACKGROUND: Fibromyalgia is a chronic widespread pain condition that is associated with sleep disturbances and cognitive impairments. Neurofeedback has been demonstrated to improve pain, sleep quality, and fatigue. However, few studies have examined the effect of neurofeedback for patients with fibromyalgia. AIM: To determine the effects of neurofeedback on pain intensity, symptom severity, sleep quality, and cognitive function in patients with fibromyalgia. DESIGN: This study was a randomized controlled trial. METHOD: Eighty participants were randomized to a neurofeedback group (N = 60), receiving sensorimotor and alpha rhythm feedback for 8 weeks, or a telephone support group (N = 20). RESULTS: Results from the generalized estimating equation modelling revealed significant group-by-time interactions for Brief Pain Inventory pain severity (B = -1.35, SE = 0.46, p = .003) and pain interference (B = -1.75, SE = 0.41, p < .001), Revised Fibromyalgia Impact Questionnaire total scores (B = -16.41, SE = 3.76, p < .001), sleep onset latency (B = -25.33, SE = 9.02, p = .005), and Psychomotor Vigilance Test error (B = -1.38, SE = 0.55, p = .013) after adjustments for age, sex, duration of illness, and group differences at baseline. CONCLUSIONS: An 8-week neurofeedback training regimen of sensorimotor rhythm and alpha brain waves significantly improved pain severity and interference, fibromyalgia symptom severity, sleep latency, and sustained attention in patients with fibromyalgia.

18.
Adv Mater ; 33(12): e2006965, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33598974

RESUMO

The electrochemical hydrogen evolution reaction (HER) is an attractive technology for the mass production of hydrogen. Ru-based materials are promising electrocatalysts owing to the similar bonding strength with hydrogen but much lower cost than Pt catalysts. Herein, an ordered macroporous superstructure of N-doped nanoporous carbon anchored with the ultrafine Ru nanoclusters as electrocatalytic micro/nanoreactors is developed via the thermal pyrolysis of ordered macroporous single crystals of ZIF-8 accommodating Ru(III) ions. Benefiting from the highly interconnected reticular macro-nanospaces, this superstrucure affords unparalleled performance for pH-universal HER, with order of magnitude higher mass activity compared to the benchmark Pt/C. Notably, an exceptionally low overpotential of only 13 mV@10 mA cm-2 is required for HER in alkaline solution, with a low Tafel slope of 40.41 mV dec-1 and an ultrahigh turnover frequency value of 1.6 H2 s-1 at 25 mV, greatly outperforming Pt/C. Furthermore, the hydrogen generation rates are almost twice those of Pt/C during practical overall alkaline water splitting. A solar-to-hydrogen system is also demonstrated to further promote the application. This research may open a new avenue for the development of advanced electrocatalytic micro/nanoreactors with controlled morphology and excellent performance for future energy applications.

19.
Food Chem ; 342: 128537, 2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33183876

RESUMO

Three water-soluble polysaccharides (UPPs 1-3) were obtained from edible green alga Ulva pertusa. The chemico-physical analyses indicated that UPPs 1-3 possessed molecular weights of 376.7 kDa, 57.21 kDa, and 131.13 kDa, with sulfate contents of 26.01 ± 8.13%, 9.86 ± 3.24%, and 13.32 ± 6.56%, respectively, and composed of arabinose, galactose, glucose, xylose, galacturonic acid, glucuronic acid, and mannuronic acid, with different ratios. The in vitro studies revealed that UPP-1 showed significant effects on the proliferation and phagocytic activity of macrophage, release of nitric oxide, and secretion of cytokines (TNF-α and IL-6). The transcript-metabolite analysis of UPP-1 treated macrophage revealed 4747 differential genes (2416 up-regulated and 2331 down-regulated) and 94 differential metabolites (77 up-regulated and 17 down-regulated) that significantly co-mapped a transcript-metabolite correlation network of biosynthesis of amino acids, glycerophospholipid metabolism, and carbon metabolism. Thus, these findings provide a valuable foundation for the potential application of U. pertusa polysaccharides.


Assuntos
Fatores Imunológicos/farmacologia , Macrófagos/efeitos dos fármacos , Polissacarídeos/química , Ulva/metabolismo , Animais , Proliferação de Células/efeitos dos fármacos , Citocinas/metabolismo , Regulação para Baixo/efeitos dos fármacos , Fatores Imunológicos/química , Macrófagos/imunologia , Macrófagos/metabolismo , Camundongos , Peso Molecular , Fagocitose/efeitos dos fármacos , Polissacarídeos/metabolismo , Polissacarídeos/farmacologia , Células RAW 264.7 , Espectroscopia de Infravermelho com Transformada de Fourier , Sulfatos/análise , Sulfatos/química , Regulação para Cima/efeitos dos fármacos
20.
Environ Sci Pollut Res Int ; 28(10): 12766-12779, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33094457

RESUMO

This study presents a systematic on-site remediation case involving both heavy metal and organic contaminants in soil and groundwater in a historically industrial-used site in Shanghai, China. Lab-scale experiments and field tests were conducted to determine the optimum parameters for the removal of contaminants in soil and groundwater. It has been found that the remediation goal of hexavalent chromium in soil could be achieved with the mass content of added sodium hydrosulfite and ferrous sulfate reaching 3% + 6%. The total chromium in the groundwater was effectively removed, when the mass ratio of sodium metabisulfite was not less than 3 g/L, and the added quick lime made pH value not less than 9. The concentrations of arsenic and 1,2-dichloropropane in the groundwater decreased evidently after extraction and mixing of groundwater. The pH and calcium chloride dosage added should be larger than 9.5 and 5 g/L, respectively, to remove phosphate in groundwater. The removal efficiency of those contaminants was examined and evaluated after the on-site remediation. The results demonstrated that it was feasible to use the chemical reduction and solidification/stabilization methods for the on-site ex situ remediation of this site, which could be referenced for the realistic remediation of similar sites.


Assuntos
Recuperação e Remediação Ambiental , Água Subterrânea , Poluentes do Solo , Poluentes Químicos da Água , China , Solo , Poluentes do Solo/análise , Poluentes Químicos da Água/análise
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