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1.
J Mol Model ; 27(10): 285, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34524545

RESUMO

The mechanism of formic acid decomposition on the Pd(111) surface has been investigated by several theoretical methods in previous studies, including PBE and PW91. These results indicated that the mechanism is different from different methods, and even by using the same method (i.e., PBE), the mechanism is also different. In this study, we have revisited the formic acid decomposition on Pd(111) surface by using another density functional RPBE and by including van der Waals interaction which is neglected in the previous studies. Our results showed that the formic acid is decomposed via O-H bond cleavage to form bi-HCOO*, and the most favorable pathway is HCOOH* → bi-HCOO* + H* → CO2* + 2H*. The energy barrier is 0.55 eV at the rate-determining step. This conclusion is consistent with one of the PBE study. This demonstrated that computational methods have a great influence on the reaction mechanism, and care should be taken in selecting the appropriate computational methods.

2.
Int J Med Sci ; 18(15): 3533-3543, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34522180

RESUMO

Importance: Despite the availability of a vaccine against the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), humans will have to live with this virus and the after-effects of the coronavirus disease 2019 (COVID-19) infection for a long time. Cholesterol plays an important role in the infection and prognosis of SARS-CoV-2, and the study of its mechanism is of great significance not only for the treatment of COVID-19 but also for research on generic antiviral drugs. Observations: Cholesterol promotes the development of atherosclerosis by activating NLR family pyrin domain containing 3 (NLRP3), and the resulting inflammatory environment indirectly contributes to COVID-19 infection and subsequent deterioration. In in vitro studies, membrane cholesterol increased the number of viral entry sites on the host cell membrane and the number of angiotensin-converting enzyme 2 (ACE2) receptors in the membrane fusion site. Previous studies have shown that the fusion protein of the virus interacts with cholesterol, and the spike protein of SARS-CoV-2 also requires cholesterol to enter the host cells. Cholesterol in blood interacts with the spike protein to promote the entry of spike cells, wherein the scavenger receptor class B type 1 (SR-B1) plays an important role. Because of the cardiovascular protective effects of lipid-lowering therapy and the additional anti-inflammatory effects of lipid-lowering drugs, it is currently recommended to continue lipid-lowering therapy for patients with COVID-19, but the safety of extremely low LDL-C is questionable. Conclusions and Relevance: Cholesterol can indirectly increase the susceptibility of patients to SARS-CoV-2 and increase the risk of death from COVID-19, which are mediated by NLRP3 and atherosclerotic plaques, respectively. Cholesterol present in the host cell membrane, virus, and blood may also directly participate in the virus cell entry process, but the specific mechanism still needs further study. Patients with COVID-19 are recommended to continue lipid-lowering therapy.


Assuntos
COVID-19/complicações , Hipercolesterolemia/complicações , Enzima de Conversão de Angiotensina 2/metabolismo , Antivirais/uso terapêutico , Aterosclerose/fisiopatologia , COVID-19/diagnóstico , COVID-19/tratamento farmacológico , COVID-19/terapia , Membrana Celular/metabolismo , HDL-Colesterol/metabolismo , LDL-Colesterol/metabolismo , Endocitose , Humanos , Hipercolesterolemia/diagnóstico , Hipercolesterolemia/terapia , Inflamação , Macrófagos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/sangue , Prognóstico , SARS-CoV-2 , Receptores Depuradores Classe B/metabolismo
3.
BMC Cardiovasc Disord ; 21(1): 452, 2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34537003

RESUMO

BACKGROUND: Light-chain cardiac amyloidosis (AL-CA) has been highly valued in developed countries, but in developing countries, the recognition and diagnosis of this condition is still limited. There are currently few reports on a large number of Chinese patients with AL-CA. The present study aimed to report real-world clinical characteristics and prognosis of AL-CA in China. METHODS AND RESULTS: Consecutive patients with AL-CA diagnosed at the Second Xiangya Hospital of Central South University between June 2012 and September 2020 were reviewed. A total of 170 patients with AL-CA have been recruited, whose mean ages were 60.81 ± 10.46. 70.59% of the patients were male. They were from eight provinces in southern China, 55.7% were referred patients, and 37.3% had been misdiagnosed previously. 64 (37.6%) patients received chemotherapy. The median survival time for patients with AL-CA was 8.00 months, and survival time for patients who received chemotherapy was 13.00 months, which was significantly longer than that of patients with palliative treatment (13.00 vs 6.00, p = 0.004). CONCLUSIONS: Although clinicians have improved their understanding of AL-CA in recent years, the prognosis of AL-CA is still poor, and the misdiagnosis rate and missed diagnosis rate are still very high in China. It is imperative to improve the recognition and early diagnosis of this condition, which may require multidisciplinary collaboration among cardiologists, hematologists and nephrologists.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34261304

RESUMO

In dual-ion batteries (DIBs) utilizing organic electrolyte solutions, the combinations of electrolyte salt and solvent serve as a determiner of the electrochemical performance of graphite electrode partially because solvent participates in the anion intercalation procedure. In this case, every solvated-anion@graphite intercalation compound possesses the characteristic intercalated gallery height. However, in Li/graphite DIBs with concentrated LiTFSI (lithium bis(trifluoromethanesulfonimide))-alkyl phosphates (ca. trimethyl, triethyl, tripropyl, tributyl phosphate) solutions, solvated-TFSI-@graphite intercalation compounds have the nearly identical intercalated gallery height of about 0.803 nm, which may be mainly attributed to both the big size of TFSI- anion and the rigid PO4 framework of these alkyl phosphate solvents.

5.
Gene Ther ; 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34257417

RESUMO

Mutations in the gene for Retinitis Pigmentosa GTPase Regulator (RPGR) cause the X-linked form of inherited retinal degeneration, and the majority are frameshift mutations in a highly repetitive, purine-rich region of RPGR known as the OFR15 exon. Truncation of the reading frame in this terminal exon ablates the functionally important C-terminal domain. We hypothesized that targeted excision in ORF15 by CRISPR/Cas9 and the ensuing repair by non-homologous end joining could restore RPGR reading frame in a portion of mutant photoreceptors thereby correcting gene function in vivo. We tested this hypothesis in the rd9 mouse, a naturally occurring mutant line that carries a frameshift mutation in RPGRORF15, through a combination of germline and somatic gene therapy approaches. In germline gene-edited rd9 mice, probing with RPGR domain-specific antibodies demonstrated expression of full length RPGRORF15 protein. Hallmark features of RPGR mutation-associated early disease phenotypes, such as mislocalization of cone opsins, were no longer present. Subretinal injections of the same guide RNA (sgRNA) carried in AAV sgRNA and SpCas9 expression vectors restored reading frame of RPGRORF15 in a subpopulation of cells with broad distribution throughout the retina, confirming successful correction of the mutation. These data suggest that a simplified form of genome editing mediated by CRISPR, as described here, could be further developed to repair RPGRORF15 mutations in vivo.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34075754

RESUMO

The pivotal steps for the practical application of dehydrogenation of aluminum hydride (AlH3) have been to decrease the temperature and increase the content of AlH3. Herein, the initial dehydrogenation temperature of AlH3 decreased to 43 °C with the amount of released hydrogen of 8.3 wt % via introducing TiO2 and Pr6O11 with synergistic catalysis effects, and its apparent activation energy of the dehydrogenation reaction decreased to 56.1 kJ mol-1, which is 52% lower than that of pure AlH3. These differences in performances of the samples are further evaluated by determining the electron density of Al-H bonds during dehydrogenation. The multiple valence state conversions of TiO2 and Pr6O11 promoted the electron transfer of H in AlH3, and a novel dehydrogenation pathway of PrH2.37 formed simultaneously, which could accelerate the breakage of Al-H bonds. The density functional theory calculations further exhibit that there are fewer electrons around H in AlH3 and the Al-H bond energy is weaker at the atomic levels, which is more conducive to the release of hydrogen. A higher hydrogen storage capacity and a lower dehydrogenation temperature make AlH3 one of the most promising hydrogen source media for mobile applications.

7.
Int Heart J ; 62(3): 540-545, 2021 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-33952805

RESUMO

This study aims to evaluate the impact of the coronavirus disease 2019 (COVID-19) pandemic on patient admissions to Hunan's cardiac intensive care units (CCUs).We conducted a retrospective, single-center study. Data were collected from patients who were confirmed to have critical cardiovascular disease and admitted to the CCU of the Second Xiangya Hospital of Central South University, Hunan, from January 23 to April 23, 2020. Compared with the same period in 2019, the results show that the number of hospitalization decreased by 19.6%; the inhospital mortality rate of CCU was decreased (28.57% versus 16.67%; odds ratio (OR), 0.50; 95% confidence interval (CI), 0.251-0.996; P = 0.047); hospital stay was decreased (7.97 versus 12.36, P < 0.001); hospital emergency percutaneous coronary intervention (PCI) rate in patients with acute coronary syndromes (ACS) significantly decreased (76.00% versus 39.00%, P < 0.001); among this, the PCI rate of patients with ST-segment elevation myocardial infarction (STEMI) decreased (76.32% versus 55.17%, P = 0.028) as well. In addition, the number of patients transferred from other hospitals significantly decreased (76.79% versus 56.67%, P = 0.002), and the number of patients transferred from other cities also decreased by 10.75%.During the outbreak of the COVID-19 epidemic in Hunan Province, the number of patients admitted to CCU decreased, as well as the mortality rate; fewer patients with severe cardiovascular disease can be transported to better hospitals from remote rural areas. In addition to epidemic prevention and control, experts in China should focus on improved emergency transport medical services to reduce this impact.


Assuntos
COVID-19 , Doenças Cardiovasculares/mortalidade , Unidades de Cuidados Coronarianos/tendências , Mortalidade Hospitalar/tendências , Admissão do Paciente/tendências , Transferência de Pacientes/tendências , Adulto , Idoso , Idoso de 80 Anos ou mais , COVID-19/epidemiologia , COVID-19/prevenção & controle , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/terapia , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Estudos Retrospectivos
8.
Exp Gerontol ; 150: 111345, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-33836261

RESUMO

High-intensity interval training (HIIT) can effectively increase peak oxygen consumption, body composition, physical fitness, and health-related characteristics of adults; however, its impact in the older population remains highly debated. This review and meta-analysis aimed to evaluate the effects of high-intensity interval training on cardiorespiratory fitness, body composition, physical fitness, and health-related outcomes in older adults. Four electronic databases (PubMed, Scopus, Medline, and Web of Science) were searched (until July 2020) for randomized trials comparing the effect of HIIT on physical fitness, metabolic parameters, and cardiorespiratory fitness in older adults. The Cochrane risk of bias assessment tool was used to evaluate the methodological quality of the included studies; Stata 14.0 software was used for statistical analysis. HIIT significantly improved the maximum rate of oxygen consumption (VO2peak) as compared to a moderate-intensity continuous training (MICT) protocol (HIIT vs. MICT: weighted mean difference = 1.74, 95% confidence interval: 0.80-2.69, p < 0.001). Additional subgroup analyses determined that training periods >12 weeks, training frequencies of 2 sessions/week, session lengths of 40 min, 6 sets and repetitions, training times per repetition of >60 s, and rest times of <90 s were more effective for VO2peak. This systematic review and meta-analysis showed that HIIT induces favorable adaptions in cardiorespiratory fitness, physical fitness, muscle power, cardiac contractile function, mitochondrial citrate synthase activity, and reduced blood triglyceride and glucose levels in older individuals, which may help to maintain aerobic fitness and slow down the process of sarcopenia.


Assuntos
Aptidão Cardiorrespiratória , Treinamento Intervalado de Alta Intensidade , Idoso , Composição Corporal , Humanos , Aptidão Física , Ensaios Clínicos Controlados Aleatórios como Assunto
9.
Stem Cell Reports ; 16(2): 252-263, 2021 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-33513359

RESUMO

Mutations in the photoreceptor transcription factor gene cone-rod homeobox (CRX) lead to distinct retinopathy phenotypes, including early-onset vision impairment in dominant Leber congenital amaurosis (LCA). Using induced pluripotent stem cells (iPSCs) from a patient with CRX-I138fs48 mutation, we established an in vitro model of CRX-LCA in retinal organoids that showed defective photoreceptor maturation by histology and gene profiling, with diminished expression of visual opsins. Adeno-associated virus (AAV)-mediated CRX gene augmentation therapy partially restored photoreceptor phenotype and expression of phototransduction-related genes as determined by single-cell RNA-sequencing. Retinal organoids derived from iPSCs of a second dominant CRX-LCA patient carrying K88N mutation revealed the loss of opsin expression as a common phenotype, which was alleviated by AAV-mediated augmentation of CRX. Our studies provide a proof-of-concept for developing gene therapy of dominant CRX-LCA and other CRX retinopathies.

10.
Adv Drug Deliv Rev ; 168: 181-195, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32603815

RESUMO

A variety of inherited or multifactorial ocular diseases call for novel treatment paradigms. The newly developed genome editing technology, CRISPR, has shown great promise in treating these diseases, but delivery of the CRISPR/Cas components to target ocular tissues and cells requires appropriate use of vectors and routes of administration to ensure safety, efficacy and specificity. Although adeno-associated viral (AAV) vectors are thus far the most commonly used tool for ocular gene delivery, sustained expression of CRISPR/Cas components may cause immune reactions and an increased risk of off-target editing. In this review, we summarize the ocular administration routes and discuss the advantages and disadvantages of viral and non-viral vectors for delivery of CRISPR/Cas components to the eye. We review the existing studies of CRISPR/Cas genome editing for ocular diseases and discuss the major challenges of the technology in ocular applications. We also discuss the most recently developed CRISPR tools such as base editing and prime editing which may be used for future ocular applications.

11.
Nat Microbiol ; 6(1): 51-58, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33199863

RESUMO

Coronavirus disease 2019 (COVID-19) is caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1-3 and individuals with COVID-19 have symptoms that can be asymptomatic, mild, moderate or severe4,5. In the early phase of infection, T- and B-cell counts are substantially decreased6,7; however, IgM8-11 and IgG12-14 are detectable within 14 d after symptom onset. In COVID-19-convalescent individuals, spike-specific neutralizing antibodies are variable3,15,16. No specific drug or vaccine is available for COVID-19 at the time of writing; however, patients benefit from treatment with serum from COVID-19-convalescent individuals17,18. Nevertheless, antibody responses and cross-reactivity with other coronaviruses in COVID-19-convalescent individuals are largely unknown. Here, we show that the majority of COVID-19-convalescent individuals maintained SARS-CoV-2 spike S1- and S2-specific antibodies with neutralizing activity against the SARS-CoV-2 pseudotyped virus, and that some of the antibodies cross-neutralized SARS-CoV, Middle East respiratory syndrome coronavirus or both pseudotyped viruses. Convalescent individuals who experienced severe COVID-19 showed higher neutralizing antibody titres, a faster increase in lymphocyte counts and a higher frequency of CXCR3+ T follicular help (TFH) cells compared with COVID-19-convalescent individuals who experienced non-severe disease. Circulating TFH cells were spike specific and functional, and the frequencies of CXCR3+ TFH cells were positively associated with neutralizing antibody titres in COVID-19-convalescent individuals. No individuals had detectable autoantibodies. These findings provide insights into neutralizing antibody responses in COVID-19-convalescent individuals and facilitate the treatment and vaccine development for SARS-CoV-2 infection.


Assuntos
Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Anticorpos Amplamente Neutralizantes/imunologia , COVID-19/imunologia , SARS-CoV-2/imunologia , Células T Auxiliares Foliculares/imunologia , Anticorpos Neutralizantes/imunologia , Reações Cruzadas , Humanos , Receptores CXCR3/imunologia
12.
Aging (Albany NY) ; 12(24): 26248-26262, 2020 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-33232272

RESUMO

Aging is an important factor affecting the deterioration of patients with coronavirus disease 2019 (COVID-19). The aging and degeneration of various tissues and organs in the elderly lead to impaired organ function. Underlying conditions such as chronic lung disease, cardiovascular disease, and diabetes in aged patients are associated with higher mortality. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily interacts with the cell surface receptor angiotensin-converting enzyme (ACE) 2 and other accessory proteins such as 78 kDa glucose-regulated protein 78 (GRP78) and CD147. Thus, altered receptor signals in aging and chronic disease play a role in SARS-CoV-2 infection, and are associated with a higher risk of deterioration in different organs. In this review, after a brief introduction to the link between aging and receptors for SARS-CoV-2, we focus on the risk of deterioration in different organs of COVID-19 patients considering aging as the main factor. We further discuss the structural and/or physiological changes in the immune system and organs (lung, heart, kidney, vessels, nerve system), as well as those associated with diabetes, in aging patients, and speculate on the most likely mechanisms underlying the deterioration of COVID-19 patients.


Assuntos
COVID-19/epidemiologia , SARS-CoV-2 , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Enzima de Conversão de Angiotensina 2/metabolismo , Biomarcadores , COVID-19/metabolismo , COVID-19/virologia , Criança , Pré-Escolar , Comorbidade , Suscetibilidade a Doenças , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Mortalidade , Avaliação de Resultados da Assistência ao Paciente , Vigilância da População , Receptores Virais/metabolismo , Adulto Jovem
13.
Dalton Trans ; 49(40): 14180-14190, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33026010

RESUMO

The ion-exchange process is usually influenced by the surface properties of the adsorbents. In particular, the prophase adsorption/desorption process is confined by different crystal facets. In this research, spinel Li4Ti5O12 nanosheets with an exposed (1-14) high-index facet were prepared by a hydrothermal method followed by calcination treatment. Then, a H4Ti5O12 adsorbent was obtained, covered with the same (1-14) facets, after treatment with 0.2 M HCl. This special facet-exposed H4Ti5O12 has high cycling ability, with the adsorption uptake remaining at 96.84% after four cycles, a fast adsorption equilibrium time (equilibrium time < 60 min), excellent ion adsorption selectivity for Li+ uptake (separation factor: Li+ > K+ > Ca2+ > Na+ > Mg2+), and good adsorption capacity for Li+ uptake (21.57 mg g-1 ). With the help of X-ray photoelectron spectroscopy analyses, the Li+ adsorption process on the H4Ti5O12 nanosheets is shown to be an ion-exchange process. In addition, the coordination relationship between lithium and oxygen ions was investigated, illustrating that the four-coordinated structure is more stable than other complexes. These results indicate that hydrogen ions are exchanged for lithium ions at tetrahedral 8a sites, leading to the H4Ti5O12 structure with high stability in the adsorption-desorption cycling process.

14.
Transl Vis Sci Technol ; 9(7): 28, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32844051

RESUMO

Purpose: Electric micro-current has been shown to enhance penetration and transduction of adeno-associated viral (AAV) vectors in mouse retina after intravitreal administration. We termed this: "electric-current vector mobility (ECVM)." The present study considered whether ECVM could augment retinal transduction efficiency of intravitreal AAV8-CMV-EGFP in normal rabbit and nonhuman primate (NHP) macaque. Potential mechanisms underlying enhanced retinal transduction by ECVM were also studied. Methods: We applied an electric micro-current across the intact eye of normal rabbit and monkey in vivo for a brief period immediately after intravitreal injection of AAV8-CMV-EGFP. Retinal GFP expression was evaluated by fundus imaging in vivo. Retinal immunohistochemistry was performed to assess the distribution of retinal cells transduced by the AAV8-EGFP. Basic fibroblast growth factor (bFGF) was analyzed by quantitative RT-polymerase chain reaction (PCR). Müller glial reactivity and inner limiting membrane (ILM) were examined by the glial fibrillary acidic protein (GFAP) and vimentin staining in mouse retina, respectively. Results: ECVM significantly increased the efficiency of AAV reaching and transducing the rabbit retina following intravitreal injection, with gene expression in inner nuclear layer, ganglion cells, and Müller cells. Similar trend of improvement was observed in the ECVM-treated monkey eye. The electric micro-current upregulated bFGF expression in Müller cells and vimentin showed ILM structural changes in mouse retina. Conclusions: ECVM promotes the transduction efficiency of AAV8-CMV-GFP in normal rabbit and monkey retinas following intravitreal injection. Translational Relevance: This work has potential translational relevance to human ocular gene therapy by increasing retinal expression of therapeutic vectors given by intravitreal administration.


Assuntos
Dependovirus , Vetores Genéticos , Animais , Dependovirus/genética , Expressão Gênica , Vetores Genéticos/genética , Coelhos , Retina , Transdução Genética
15.
ACS Appl Mater Interfaces ; 12(39): 43560-43567, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32852199

RESUMO

Despite extensive studies, the role of polar chemical interfaces on carrier materials anchoring polysulfide species remains an ambiguous but intriguing topic for lithium-sulfur batteries. Herein, to further investigate the effect of metal sulfides in the conversion of polysulfides, three kinds of MxSy (M = Cr, Mo, and W) are chosen and prepared in the forms of two-dimensional MxSy/C composites via a method using NaCl as a template and a subsequent high-temperature sulfuration process. Compared with a blank sample, the three composites exhibit superior adsorption of soluble polysulfides and faster kinetics of the deposition of Li2S2/Li2S, especially on Cr3S4/C and WS2/C. These differences in performances of the three composites are further evaluated by the values of the Gibbs free energy in each step of polysulfide conversion. In the conversion processes of Li2S4 to Li2S2 and then to Li2S, the values are more negative on Cr3S4 and WS2, showing stronger promotion abilities for the formation of Li2S than MoS2. This work can effectively deepen the role of metal sulfides in the conversion process of polysulfides and provide valuable insights into the design of superior carriers for lithium-sulfur batteries.

16.
Cancer Med ; 9(20): 7524-7536, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32860339

RESUMO

To evaluate whether the addition of biomarkers to traditional clinicopathological parameters may help to increase the accurate prediction of prostate re-biopsy outcome. A training cohort with 98 patients and a validation cohort with 72 patients were retrospectively recruited into our study. Immunohistochemical analysis was used to evaluate the immunoreactivity of a group of biomarkers in the initial negative biopsy normal-looking tissues of the training and validation cohorts. p-STAT3, Mcm2, and/or MSR1 were selected out of 10 biomarkers to construct a biomarker index for predicting cancer and high-grade prostate cancer (HGPCa) in the training cohort based on the stepwise logistic regression analysis; these biomarkers were then validated in the validation cohort. In the training cohort study, we found that the biomarker index was independently associated with the re-biopsy outcomes of cancer and HGPCa. Moreover supplementing the biomarker index with traditional clinical-pathological parameters can improve the area under the receiver operating characteristic curve of the model from 0.722 to 0.842 and from 0.735 to 0.842, respectively, for predicting cancer and HGPCa at re-biopsy. In the decision-making analysis, we found the model supplemented with the biomarker index can improve patients' net benefit. The application of the model to clinical practice, at a 10% risk threshold, would reduce the number of biopsies by 34.7% while delaying the diagnosis of 7.8% cancers and would reduce the number of biopsies by 73.5% while delaying the diagnosis of 17.8% HGPCas. Taken together, supplementing the biomarker index with clinicopathological parameters may help urologists in re-biopsy decision-making processes.


Assuntos
Biomarcadores Tumorais , Biomarcadores , Próstata/metabolismo , Próstata/patologia , Neoplasias da Próstata/diagnóstico , Idoso , Biópsia , Tomada de Decisão Clínica , Árvores de Decisões , Gerenciamento Clínico , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias da Próstata/etiologia , Neoplasias da Próstata/terapia , Curva ROC , Reprodutibilidade dos Testes
17.
Dalton Trans ; 49(31): 10939-10948, 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32724975

RESUMO

Lithium-rich spinel lithium manganese oxide (LMO) compounds are one kind of promising adsorbents for lithium recovery from brine due to their high capacity and low Mn dissolution; Li1.6Mn1.6O4 is one of them. However, Mn3+ exists in the Li1.6Mn1.6O4 precursor due to incomplete reaction during syntheses, and the disproportionation reaction of Mn3+ inevitably results in Mn dissolution during lithium adsorption and desorption. The stable recycling and structural stability of Li1.6Mn1.6O4 were improved in aqueous lithium resources through K-gradient doping (LMO-K). The dissolution of Mn is reduced to 4.0% from 5.4% (before doping) and the adsorption capacity is kept at high capacity (31.6 mg g-1) at a low Li+ concentration of 12 mmol L-1. In addition, first-principles calculations further confirm that K substitutes for Li at 16d sites, leading to the stabilization of the Mn cations in the compound. With the help of K doping, the undesired dissolution of Mn in the cycle process is inhibited, which may be due to the reduction in the content of Mn3+ and improvement in the structural stability of the adsorbent.

18.
Angew Chem Int Ed Engl ; 59(32): 13568-13574, 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32495981

RESUMO

Synthesis of well-defined atomically mixed alloy nanoparticles on desired substrates is an ultimate goal for their practical application. Herein we report a general approach for preparing atomically mixed AuPt, AuPd, PtPd, AuPtPd NAs(nanoalloys) through single-atom level manipulation. By utilizing the ubiquitous tendency of aggregation of single atoms into nanoparticles at elevated temperatures, we have synthesized nanoalloys on a solid solvent with CeO2 as a carrier and transition-metal single atoms as an intermediate state. The supported nanoalloys/CeO2 with ultra-low noble metal content (containing 0.2 wt % Au and 0.2 wt % Pt) exhibit enhanced catalytic performance towards complete CO oxidation at room temperature and remarkable thermostability. This work provides a general strategy for facile and rapid synthesis of well-defined atomically mixed nanoalloys that can be applied for a range of emerging techniques.

19.
Angew Chem Int Ed Engl ; 59(33): 13923-13928, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32436324

RESUMO

The applications of the most promising Fe-N-C catalysts are prohibited by their limited intrinsic activities. Manipulating the Fe energy level through anchoring electron-withdrawing ligands is found effective in boosting the catalytic performance. However, such regulation remains elusive as the ligands are only uncontrollably introduced oweing to their energetically unstable nature. Herein, we report a rational manipulation strategy for introducing axial bonded O to the Fe sites, attained through hexa-coordinating Fe with oxygen functional groups in the precursor. Moreover, the O modifier is stabilized by forming the Fe-O-Fe bridge bond, with the approximation of two FeN4 sites. The energy level modulation thus created confers the sites with an intrinsic activity that is over 10 times higher than that of the normal FeN4 site. Our finding opens a novel strategy to manage coordination environments at an atomic level for high activity ORR catalysts.

20.
Mech Ageing Dev ; 189: 111262, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32422206

RESUMO

PURPOSE: This study aims to evaluate whether regular lifelong exercise has effects on age-related inflammatory cytokines, oxidative stress, and the skeletal muscle proteome. METHODS: Four groups of adult-aged (8-month-old) female Sprague Dawley rats were used: rats for which training was initiated at either 8 (8 M-MICT, moderate-intensity continuous training) or 18 months (18 M-MICT) and sedentary rats aged either 26 (26 M-SED) or 8 months (8M-SED), who served as aging and adult sedentary controls, respectively. Aged skeletal muscles were subjected to proteomic and Kyoto Encyclopedia of Genes and Genomes (KEGG) and immunoblotting analyses. RESULTS: Age-related loss of physical performance and anti-inflammatory steroid levels were lowest in the 8 M-MICT group, while the anti-oxidative activities remained unchanged compared to 18 M-MICT rats. The proteomic analysis demonstrated an amelioration of age-related changes to muscle contraction, focal adhesion signaling, mitochondrial function, apoptosis and regeneration, anti-oxidation, and protein processing in the endoplasmic reticulum in the 8 M-MICT. Additionally, neurotrophin (BDNF) and AKT/FOXO signaling pathways were upregulated in 8 M-MICT rats compared to 26 M-SED. CONCLUSION: 8 M-MICT exhibited greater beneficial effects in ameliorating age-related inflammation and physical performance loss, compared to 18 M-MICT. The amelioration is potentially related to the upregulation of autophagy activities via BDNF/AKT signaling.


Assuntos
Envelhecimento/metabolismo , Citocinas/metabolismo , Proteínas Musculares/metabolismo , Músculo Esquelético/metabolismo , Estresse Oxidativo , Condicionamento Físico Animal , Proteoma/metabolismo , Animais , Feminino , Inflamação/metabolismo , Ratos , Ratos Sprague-Dawley
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