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1.
Molecules ; 25(3)2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32028657

RESUMO

A highly efficient quinine-derived primary-amine-catalyzed asymmetric aldol addition of hydroxyacetone to arylglyoxals is described. Structurally diverse anti-2,3-dihydroxy-1,4-diones were generated in high yields, with good diastereoselectivities and enantioselectivities.

2.
J Mol Evol ; 88(2): 202-209, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31919584

RESUMO

Drug-resistant Mycobacterium tuberculosis (M. tuberculosis) has become an increasingly serious public health problem and has complicated tuberculosis (TB) treatment. Levofloxacin (LOF) is an ideal anti-tuberculosis drug in clinical applications. However, the detailed molecular mechanisms of LOF-resistant M. tuberculosis in TB treatment have not been revealed. Our study performed transcriptome and methylome sequencing to investigate the potential biological characteristics of LOF resistance in M. tuberculosis H37Rv. In the transcriptome analysis, 953 differentially expressed genes (DEGs) were identified; 514 and 439 DEGs were significantly downregulated and upregulated in the LOF-resistant group and control group, respectively. The KEGG pathway analysis revealed that 97 pathways were enriched in this study. In the methylome analysis, 239 differentially methylated genes (DMGs) were identified; 150 and 89 DMGs were hypomethylated and hypermethylated in the LOF-resistant group and control group, respectively. The KEGG pathway analysis revealed that 74 pathways were enriched in this study. The overlap study suggested that 25 genes were obtained. It was notable that nine genes expressed downregulated mRNA and upregulated methylated levels, including pgi, fadE4, php, cyp132, pckA, rpmB1, pfkB, acg, and ctpF, especially cyp132, pckA, and pfkB, which were vital in LOF-resistant M. tuberculosis H37Rv. The overlapping genes between transcriptome and methylome could be essential for studying the molecular mechanisms of LOF-resistant M. tuberculosis H37Rv. These results may provide informative evidence for TB treatment with LOF.

3.
BMJ Open ; 9(10): e031098, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31630106

RESUMO

BACKGROUND: In China, the local health insurance coverage is usually related to timely reimbursement of hypertensive care in primary care settings, while health insurance that is not local could represent an obstacle for accessibility and affordability of primary care for hypertensive patients. OBJECTIVE: To investigate whether local health insurance schemes have a positive impact on hypertension management and control. DESIGN: We performed an on-site, face-to-face, patients survey in community health centres (CHCs) in Shenzhen, China. SETTING AND PARTICIPANTS: Hypertensive patients seeking healthcare from CHCs were selected as study participants using a systematic sampling design. MAIN MEASURES: We obtained information about insurance status, social capital, drug treatment and control of hypertension. Multivariable stepwise logistic regression models were constructed to test the associations between insurance status and hypertension management, as well as insurance status and social capital. RESULTS: A total of 867 participants were included in the final study analysis. We found that the participants covered by local insurance schemes were more likely to be managed in primary care facilities (61.1% vs 81.9%; OR=2.58, 95% CI: 1.56 to 4.28), taking antihypertensive drugs (77.2% vs 88.0%; OR=2.23, 95% CI: 1.37 to 3.62) and controlling blood pressure (43.0% vs 52.4%; OR=1.46, 95% CI: 1.03 to 2.07) when compared with those with insurance coverage that is not local. The participants covered by local insurance schemes reported a higher score of perceived generalised trust than those without (4.23 vs 3.97; OR=0.74, 95% CI: 0.53 to 0.86). CONCLUSION: Our study demonstrates that local health insurance coverage could help improve management and control of hypertension in a primary care setting. Policymakers suggest initiating social interventions for better management and control of hypertension at the primary care level, although the causal pathways across insurance status, social capital and control of hypertension deserve further investigations.

4.
Int Immunopharmacol ; 75: 105827, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31454695

RESUMO

OBJECTIVE: Numerous studies have investigated associations of gene polymorphisms and circulating levels of TNF-α with ischemic stroke (IS), but the results were controversial. The aims of this study were to systematically evaluate these associations. METHODS: Relevant publications were retrieved by searching databases. Odds ratios (ORs) and standard mean differences (SMDs) with 95% confidence intervals (95% CIs) were used to assess the association of the TNF-α gene and cytokine with IS, respectively. The Cochrane Q test and I2 statistic were used to test heterogeneity. Subgroup analysis and publication bias were performed. RESULTS: 25 and 9 articles examined the association of polymorphisms and levels of the TNF-α with IS risk, respectively. Rs1800629 polymorphism was associated with IS susceptibility (OR (95% CI) =0.82 (0.72, 0.95)), especially in Asians (OR (95% CI) =0.75 (0.63, 0.89)); and rs1800610 was associated with IS susceptibility in Asians patients (OR (95% CI) =1.54 (1.31, 1.80)). While rs361525, rs1799964 and rs1799724 polymorphisms were not associated with IS susceptibility. The TNF-α level was elevated in IS patients (SMD (95% CI) =0.65 (0.29, 1.01)) including Asians (SMD (95% CI) =1.26 (0.49, 2.03)) and Caucasians (SMD (95% CI) =0.26 (0.03, 0.49)). In addition, increased level occurred in patients' serum (SMD (95% CI) =0.54 (0.08, 1.01)). CONCLUSIONS: Rs1800629 and rs1800610 polymorphisms were elucidated to be a protective factor for IS (especially in Asians) and a risk factor for Asians patients, respectively. The TNF-α level was elevated in IS, indicating that TNF-α plays an important role in the pathogenesis of IS and is a promising therapeutic target for IS.


Assuntos
Isquemia Encefálica/sangue , Isquemia Encefálica/genética , Predisposição Genética para Doença , Acidente Vascular Cerebral/sangue , Acidente Vascular Cerebral/genética , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/genética , Humanos , Polimorfismo de Nucleotídeo Único
5.
Curr Med Sci ; 39(4): 589-596, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31346995

RESUMO

The diagnosis and treatment of fever of unknown origin (FUO) are huge challenges to clinicians. Separating the etiologies of FUO into infectious and non-infectious disease is conducive to clinical physicians not only on making decisions rapidly concerning the prescription of suitable antibiotics but also on further analysis of the final diagnosis. In order to develop and validate a diagnostic tool to efficiently distinguish the etiologies of adult FUO patients as infectious or non-infectious disease, FUO patients from the departments of infectious disease and internal medicine in three Chinese tertiary hospitals were enrolled retrospectively and prospectively. By using polynomial logistic regression analysis, the diagnostic formula and the associated scoring system were developed. The variables included in this diagnostic formula were from clinical evaluations and common laboratory examinations. The proposed tool could discriminate infectious and non-infectious causes of FUO with an area under receiver operating characteristic curve (AUC) of 0.83, sensitivity of 0.80 and specificity of 0.75. This diagnosis tool could predict the infectious and non-infectious causes of FUO in the validation cohort with an AUC of 0.79, sensitivity of 0.79 and specificity of 0.70. The results suggested that this diagnostic tool could be a reliable tool to discriminate between infectious and non-infectious causes of FUO.


Assuntos
Doenças Transmissíveis/diagnóstico , Febre de Causa Desconhecida/diagnóstico , Doenças não Transmissíveis/epidemiologia , Adulto , China/epidemiologia , Doenças Transmissíveis/epidemiologia , Doenças Transmissíveis/patologia , Diagnóstico Diferencial , Febre de Causa Desconhecida/epidemiologia , Febre de Causa Desconhecida/patologia , Humanos , Modelos Logísticos
6.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1938-1945, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342724

RESUMO

To systemically evaluate the clinical efficacy and safety of traditional Chinese medicine( TCM) external application combined with three-step analgesic therapy in treating primary liver cancer pain. CNKI,Wanfang,CBM,VIP,Medline and Cochrane Library and manual retrieval were used to search for the clinical randomized controlled trials on TCM external applications combined with three-step analgesic therapy in treating primary liver cancer pain from database establishment to January,2018. The bias risk of RCTs was assessed by using the Cochrane system evaluator's Manual,and the extracted data were analyzed by using Review Manager 5. 3. Finally sixteen Chinese articles were enrolled,including one high quality article and 1 164 patients. Meta-analysis showed that TCM external applications combined with three-step analgesic therapy could alleviate the cancer pain( OR = 3. 44,95% CI[2. 49,4. 75],P <0. 000 01); prolong pain relief time( SMD = 3. 42,95%CI[1. 83,6. 40],Z = 3. 85,P = 0. 000 1); and improve the cartesian score of the patients( OR = 3. 42,95%CI[1. 83,6. 40],P = 0. 000 01). Descriptive analysis showed that the intervention may effectively shorten the onset time of pain relief,reduce VAS and NRS scores,reduce the dose of morphine,and reduce the number of bursts of pain. At present,the evidences have shown that the combination of TCM external applications combined with three-step analgesic therapy in treating primary liver cancer pain has superior clinical efficacy as compared with the three-step analgesic therapy alone. However,the clinical trials of existing small-sized randomized controlled trials have low quality of methodology and require a large sample of high quality clinical trials for further validation.


Assuntos
Analgésicos/uso terapêutico , Neoplasias Hepáticas/complicações , Medicina Tradicional Chinesa , Manejo da Dor , Dor/etiologia , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
7.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(2): 182-184, 2019 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-31184053

RESUMO

OBJECTIVE: To study the epidemiological characteristics of imported malaria in Chenzhou City, Hunan Province, so as to provide the reference for consolidating the malaria elimination and formulating the prevention and control strategies of imported malaria. METHODS: The epidemiological characteristics of imported malaria were statistically analyzed in Chenzhou City from 2010 to 2017. RESULTS: Totally 46 malaria cases, which were all imported, were reported in Chenzhou City from 2010 to 2017, with an average annual incidence of 0.12/105. The reported malaria cases were mainly falciparum malaria, accounting for 60.87% of the total number of cases. There was no obvious seasonal distribution of malaria cases, but the top of reported cases were in June. Totally 73.91% of malaria cases were concentrated in Beihu District, Suxian District, Guiyang County and Zixing City. These cases were mainly the young and middle-aged and 69.57% of the cases were from 36 to 60 years old. There was a statistically significant difference in the distribution of malaria patients among the age groups (χ2 = 47.80, P < 0.01). The median time from onset to diagnosis was 6 days, and the case confirmed institutions were dominated by municipal and above medical institutions, accounting for 52.17% of the total number of cases. There was a statistically significant difference in the proportion of confirmed cases among medical and health institutions at all levels ( χ2 = 41.96, P < 0.01). CONCLUSIONS: The importation of malaria in Chenzhou City is still severe. The awareness of malaria diagnosis and treatment in primary medical institutions, malaria patients' serum tests, and the health education of malaria control and prevention knowledge should be strengthened to consolidate the malaria elimination results.


Assuntos
Doenças Transmissíveis Importadas , Malária , Adulto , China/epidemiologia , Doenças Transmissíveis Importadas/epidemiologia , Doenças Transmissíveis Importadas/parasitologia , Humanos , Incidência , Malária/epidemiologia , Malária/prevenção & controle , Pessoa de Meia-Idade , Fatores de Risco
8.
Chemistry ; 25(46): 10845-10857, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31210396

RESUMO

The synthesis and characterization of 10-(dimesitylboryl)-N,N-di-p-tolylbenzo[c]phenanthren-4-amine (3-B(Mes)2 -[4]helix-9-N(p-Tol)2 1) and 13-(dimesitylboryl)-N,N-di-p-tolyldibenzo[c,g]phenanthren-8-amine (3-B(Mes)2 -[5]helix-12-N(p-Tol)2 2) are reported herein. Their electrochemical and photophysical properties have been studied experimentally and theoretically. The donor and acceptor-substituted helicene derivatives exhibit moderate fluorescence quantum yields in THF (Φf =0.48 and 0.61 for 1 and 2, respectively), which are higher than unsubstituted ones (Φf =0.18 for [4]helicene; Φf <0.05 for [n]helicenes (n≥5)). In the solid state, the Φf values are higher (Φf =1.00 and 0.55 for 1 and 2, respectively) than those in solution, most likely due to the restrictions of molecular motions. The S1 ←S0 transitions of 1 and 2 are predominately HOMO→LUMO transitions. Upon excitation with UV light, the interplanar angle between the two terminal aryl rings of the [5]helix core of 2 decreases (S1 state compared with S0 state), which is similar to placing a spring under an external force.

9.
JAMA Surg ; 154(7): e190972, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31042283

RESUMO

Importance: It is important to develop a surgical technique to reduce dissemination of tumor cells into the blood during surgery. Objective: To compare the outcomes of different sequences of vessel ligation during surgery on the dissemination of tumor cells and survival in patients with non-small cell lung cancer. Design, Setting, and Participants: This multicenter, randomized clinical trial was conducted from December 2016 to March 2018 with patients with non-small cell lung cancer who received thoracoscopic lobectomy in West China Hospital, Daping Hospital, and Sichuan Cancer Hospital. To further compare survival outcomes of the 2 procedures, we reviewed the Western China Lung Cancer database (2005-2017) using the same inclusion criteria. Interventions: Vein-first procedure vs artery-first procedure. Main Outcomes and Measures: Changes in folate receptor-positive circulating tumor cells (FR+CTCs) after surgery and 5-year overall, disease-free, and lung cancer-specific survival. Results: A total of 86 individuals were randomized; 22 patients (25.6%) were younger and 64 (74.4%) older than 60 years. Of these, 78 patients were analyzed. After surgery, an incremental change in FR+CTCs was observed in 26 of 40 patients (65.0%) in the artery-first group and 12 of 38 (31.6%) in the vein-first group (P = .003) (median change, 0.73 [interquartile range (IQR), -0.86 to 1.58] FU per 3 mL vs -0.50 [IQR, -2.53 to 0.79] FU per 3 mL; P = .006). Multivariate analysis confirmed that the artery-first procedure was a risk factor for FR+CTC increase during surgery (hazard ratio [HR], 4.03 [95% CI, 1.53-10.63]; P = .005). The propensity-matched analysis included 420 patients (210 with vein-first procedures and 210 with artery-first procedures). The vein-first group had significantly better outcomes than the artery-first group for 5-year overall survival (73.6% [95% CI, 64.4%-82.8%] vs 57.6% [95% CI, 48.4%-66.8%]; P = .002), disease-free survival (63.6% [95% CI, 55.4%-73.8%] vs 48.4% [95% CI, 40.0%-56.8%]; P = .001), and lung cancer-specific survival (76.4% [95% CI, 67.6%-85.2%] vs 59.9% [95% CI, 50.5%-69.3%]; P = .002). Multivariate analyses revealed that the artery-first procedure was a prognostic factor of poorer 5-year overall survival (HR, 1.65 [95% CI, 1.07-2.56]; P = .03), disease-free survival (HR, 1.43 [95% CI, 1.01-2.04]; P = .05) and lung cancer-specific survival (HR = 1.65 [95% CI, 1.04-2.61]; P = .03). Conclusions and Relevance: Ligating effluent veins first during surgery may reduce tumor cell dissemination and improve survival outcomes in patients with non-small cell lung cancer. Trial Registration: ClinicalTrials.gov identifier: NCT03436329.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/cirurgia , Neoplasias Pulmonares/cirurgia , Células Neoplásicas Circulantes/patologia , Pneumonectomia/métodos , Artéria Pulmonar/cirurgia , Veias Pulmonares/cirurgia , Sistema de Registros , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Ligadura , Neoplasias Pulmonares/diagnóstico , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Estudos Prospectivos , Fatores de Risco , Toracoscopia/métodos , Tomografia Computadorizada de Emissão de Fóton Único , Tomografia Computadorizada por Raios X , Resultado do Tratamento
10.
J Matern Fetal Neonatal Med ; : 1-4, 2019 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-30890010

RESUMO

OBJECTIVE: Although epidural analgesia is widely used during labor, its impact on breastfeeding has not yet reached a consensus. This retrospective cohort study was to investigate the association of patient-controlled epidural analgesia (PCEA) during labor with breastfeeding initiation and continuation. METHODS: Medical records from 1 February, 2016 to 31 December, 2016 at Guangzhou Women and Children's Medical Center, China were reviewed for women received PCEA or not. Breastfeeding continuation was assessed by a questionnaire at 6 months after hospital discharge. RESULTS: Nine hundred twenty-two women were enrolled in the study, with 527 of these women received PCEA for labor analgesia. The proportion of timely initiation of breastfeeding (within 1 h after birth), and exclusive or partial breastfeeding at any of the evaluation time points (1, 3, and 6 months) between two groups showed no statistically significant difference. CONCLUSION: Our data do not support an association between the PCEA and discontinuation of breastfeeding within 6 months postpartum.

11.
Mol Ther Nucleic Acids ; 14: 509-519, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30753994

RESUMO

The influenza A virus poses serious public health challenges worldwide. Strikingly, small noncoding microRNAs (miRNAs) that modulate gene expression are closely involved in antiviral responses, although the underlying mechanisms are essentially unknown. We now report that microRNA-340 (miR340) is downregulated following influenza A and other RNA virus infections, implying that host cells deplete miR340 as an antiviral defense mechanism. Accordingly, the inhibition or knockdown of endogenous miR340 clearly prevents the infection of cultured cells, whereas the forced expression of miR340 significantly enhances virus replication. Using next-generation sequencing, we found that miR340 attenuates cellular antiviral immunity. Moreover, mechanistic studies defined miR340 as a repressor of RIG-I and OAS2, critical factors for the establishment of an antiviral response. Collectively, these data indicate that host cells may lower their viral loads by regulating miRNA pathways, which may, in turn, provide new opportunities for treatment.

12.
Curr Med Sci ; 38(6): 1025-1031, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30536065

RESUMO

The present study aimed to establish a list of parameters indicative of pathogen invasion and develop a predictive model to distinguish the etiologies of fever of unknown origin (FUO) into infectious and non-infectious causes. From January 2014 to September 2017, 431 patients with FUO were prospectively enrolled in the study population. This study established a list of 26 variables from the following 4 aspects: host factors, epidemiological factors, behavioral factors, and iatrogenic factors. Predefined predicted variables were included in a multivariate logistic regression analysis to develop a predictive model. The predictive model and the corresponding scoring system were developed using data from the confirmed diagnoses and 9 variables were eventually identified. These factors were incorporated into the predictive model. This model discriminated between infectious and non-infectious causes of FUO with an AUC of 0.72, sensitivity of 0.71, and specificity of 0.63. The predictive model and corresponding scoring system based on factors concerning pathogen invasion appear to be reliable screening tools to discriminate between infectious and non-infectious causes of FUO.


Assuntos
Doenças Transmissíveis/diagnóstico , Febre de Causa Desconhecida/diagnóstico , Feminino , Humanos , Pessoa de Meia-Idade , Estudos Prospectivos , Sensibilidade e Especificidade
13.
Biomed Pharmacother ; 108: 60-64, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30216801

RESUMO

Voriconazole (VRC) and proton pump inhibitors (PPIs) have similar metabolic pathways. The objectives of the study are to evaluate the impact of PPIs on the pharmacokinetics of VRC. Human liver microsomes model was applied to assess the inhibitory effects of PPIs on the metabolism of VRC in vitro. A retrospective study was also carried out to explore the relationship between the plasma VRC trough concentrations and PPIs uses. Patients were divided into six groups: control (n = 166), lansoprazole (LAN, n = 38), esomeprazole (ESO, n = 19), omeprazole (OME, n = 45), pantoprazole (PAN, n = 43), and ilaprazole (ILA, n = 38) groups. All five PPIs showed concentration-dependent inhibitory effects on the VRC metabolism in human liver microsomes, among which LAN, OME and ESO were three of the most potent inhibitors. Consistently, co-administered with LAN, OME and ESO significantly increased the plasma VRC trough levels (p < 0.05), whereas there was no significant association between VRC concentrations and PAN or ILA use. Interestingly, patients in the PPIs groups were more likely to reach the therapeutic VRC range of 1-5.5 µg/mL in steady state when compared with control patients (75-81% VS 69%). In conclusion, although all PPIs showed inhibitory effects on the VRC metabolism in vitro, only LAN, OME and ESO significantly increased VRC plasma concentrations. This study should be helpful for choice of the type of PPIs for patients administered with VRC.


Assuntos
Inibidores da Bomba de Prótons/farmacologia , Voriconazol/farmacocinética , Adulto , Feminino , Humanos , Masculino , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , Pessoa de Meia-Idade , Voriconazol/sangue , Voriconazol/metabolismo
15.
Sci Total Environ ; 636: 1139-1148, 2018 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-29913576

RESUMO

People living near roadways are exposed to high concentrations of ultrafine particles (UFP, diameter < 100 nm). This can result in adverse health effects such as respiratory illness and cardiovascular diseases. However, accurately characterizing the UFP number concentration requires expensive sets of instruments. The development of an UFP surrogate with cheap and convenient measures is needed. In this study, we used a mobile measurement platform with a Fast Mobility Particle Sizer (FMPS) and sound level meter to investigate the spatiotemporal relations of noise and UFP and identify the hotspots of UFP. UFP concentration levels were significantly influenced by temporal and spatial variations (p < 0.001). We proposed a Generalized Additive Models to predict UFP number concentration in the study area. The model uses noise and meteorological covariates to predict the UFP number concentrations at an industrial site in Taichung, Taiwan. During the one year sampling campaign from fall 2013 to summer 2014, mobile measurements were performed at least one week for each season, both on weekdays and weekends. The proposed model can explain 80% of deviance and has coefficient of determination (R2) of 0.77. Moreover, the developed UFP model was able to adequately predict UFP concentrations, and can provide people with a convenient way to determine UFP levels. Finally, the results from this study could help facilitate the future development of noise mobile measurement.


Assuntos
Poluição do Ar/estatística & dados numéricos , Monitoramento Ambiental/métodos , Modelos Químicos , Ruído , Material Particulado/análise , Poluentes Atmosféricos/análise , Tamanho da Partícula , Taiwan , Transportes , Emissões de Veículos/análise
16.
J Photochem Photobiol B ; 180: 9-16, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29413707

RESUMO

Recently, copper chalcogenide semiconductors have been reported as new near-infrared (NIR) photothermal agents. However, it is difficult to modify them with recognition molecules, and their photothermal conversion efficiencies are relatively low, making it difficult to achieve the targeted photothermal ablation of cancer cells with a high efficiency. In this study, reduced graphene oxide (rGO) was first coated on the surface of Cu2-xSe nanoparticles (NPs) to provide abundant functional groups for the next modification and to increase the photothermal conversion efficiency. Then, doxorubicin (DOX) was loaded and folic acid (FA) molecules were covalently linked onto the surface of Cu2-xSe/rGO nanocomposites. The formed DOX@Cu2-xSe@rGO-FA nanocomposites were successfully used as chemo-photothermal agents for the targeted killing of cancer cells by utilizing the recognition ability of FA, chemotherapy effect of DOX and photothermal effects of rGO and Cu2-xSe NPs. Under the 980-nm NIR laser irradiation, the nanocomposites showed significantly enhanced chemo-photothermal therapy effect, which can be potentially applied in the nanomedicine field.


Assuntos
Cobre/química , Portadores de Fármacos/química , Grafite/química , Nanopartículas Metálicas/química , Selênio/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Linhagem Celular Tumoral , Doxorrubicina/química , Doxorrubicina/farmacologia , Portadores de Fármacos/síntese química , Liberação Controlada de Fármacos , Ácido Fólico/química , Ácido Fólico/metabolismo , Células HeLa , Humanos , Raios Infravermelhos , Nanopartículas Metálicas/ultraestrutura , Microscopia Confocal , Óxidos/química , Espectrofotometria
17.
Angew Chem Int Ed Engl ; 56(32): 9463-9467, 2017 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-28626943

RESUMO

We report interesting photomechanical behaviors of the dynamic molecular crystals of (E)-2-(2,4-dichlorostyryl)benzo[d]oxazole (BOACl24). The photosalient effect of the rod-like crystal based on a metal-free olefin driven by photodimerization is observed. Moreover, the needle-like crystals of BOACl24 exhibit a reversible bending away from a UV light source. The nanofibers curl easily under UV irradiation in an organogel, in which the photo-induced rolling of a small slice occurs. This suggests that the rapid release of the accumulated strain during photodimerization may lead to a photosalient effect, and the bending or curling happens when the strain is released slowly. Notably, [2+2] cycloaddition takes place between two different conformational isomers of BOACl24 on account of the rotation of the benzoxazole ring around the C-C bond in an excited state before photodimerization. Such topo-photochemical reaction has not been reported elsewhere.

18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 48(3): 359-362, 2017 May.
Artigo em Chinês | MEDLINE | ID: mdl-28616906

RESUMO

OBJECTIVES: To retrospectively investigate the clinical characteristics, surgical treatments of the patients with lung ground-glass opacities (GGO). METHODS: All the patients, who underwent surgical resection of GGO in our department from Jan. 2013 to Dec. 2016 were retrospectively reviewed. The clinicpathological features were analyzed. RESULTS: A total of 663 patients were included in this study. The rate of malignancy was 92.6% (614/663). The diameter of GGO in benign group [(0.8±0.2) cm] was significant smaller than that in malignant group [ (1.5±0.8) cm](P<0.001). The rate of irregular margin in malignant group was far higher than that in benign group (93.8% vs. 20.4%, P<0.001), but other CT signs such as vacuole sign, plural retraction, speculation and lobulation did not show significant difference between the two groups. A total of 652 (98.3%) cases were resected by video-assisted thoracoscopic surgery (VATS), and only 11 (1.7%) cases were resected by thoracotomy. A total of 336 (50.7%) patients underwent lobectomy, 226 (34.1%) underwent segmentectomy and 101 (15.2%) undewent wedge resection. The rate of surgery-related complications was 9.0% (60/663), and one (0.2%) patient died. CONCLUSIONS: With careful selection of GGO by experienced surgeons, the rate of malignancy is very high. Surgical resection may be recommended for highly suspected malignant cases. Sublobar resection or lobcotomy by VATS can achieve good treatment effect.


Assuntos
Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/cirurgia , Humanos , Pulmão/patologia , Estudos Retrospectivos , Cirurgia Torácica Vídeoassistida , Toracotomia
19.
J Huazhong Univ Sci Technolog Med Sci ; 37(2): 253-256, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28397053

RESUMO

The applied value of serum hepcidin in differential diagnosis of infection fevers versus tumor fevers was explored. A total of 432 fever patients were selected according to the domestic fever of unknown origin (FUO) diagnostic criteria from our hospital between June 2010 and November 2013. Venous blood samples were taken on the day 1, 5, 10 after admission. The infection group (98 cases) and the tumor group (50 cases) were set up based on the clinical and laboratory findings. ELISA was used to determine the serum hepcidin and IL-6 levels. SPSS 13.0 was used for statistical analysis. Hepcidin showed obvious descending trend on the 10th day in both the bacterial infection group (66 cases) and the virus infection group (32 cases), and the descending trend was similar to that of inflammatory indexes such as procalcitonin (PCT), hypersensitive C-reactive protein (h-CRP), erythrocyte sedimentation rate (ESR), white blood cell (WBC), and ferritin. Serum hepcidin showed no obvious differences in the tumor group on the day 1, 5, 10 after admission. In the infection groups, serum hepcidin was positively correlated with IL-6 (r=0.687, P=0.000) and CRP (r=0.487, P=0.026), but had a poor correlation with blood sedimentation, ferritin, PCT and WBC (P>0.05). Monitoring dynamic changes of hepcidin and related inflammatory factors in patients with fever is expected to be used for clinical identification of infection fever and tumor fever.


Assuntos
Doenças Transmissíveis/diagnóstico , Febre/etiologia , Hepcidinas/sangue , Neoplasias/diagnóstico , Adulto , Sedimentação Sanguínea , Proteína C-Reativa/metabolismo , Doenças Transmissíveis/sangue , Doenças Transmissíveis/metabolismo , Diagnóstico Diferencial , Feminino , Febre/metabolismo , Humanos , Interleucina-6/metabolismo , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Neoplasias/sangue , Neoplasias/metabolismo
20.
Talanta ; 167: 193-200, 2017 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-28340710

RESUMO

A selective colorimetric assay for spermine was proposed in this work. In a weak alkaline medium, the conformational structure of double-stranded calf thymus DNA (ctDNA) was loosened to install gold nanoparticles (AuNPs) into chains. While, the chain assembly of AuNPs could form cross-linking aggregates when spermine was present, which was attributed to the electrostatic interaction between the positive change of spermine and negative change both of AuNPs and ctDNA, as well as the groove binding between ctDNA and spermine. Under the optimum conditions, the aggregation degree of AuNPs was proportional to the concentration of spermine in the range of 0.1-2.0µM with a limit of detection of 11.6nM. More interestingly, AuNPs changed from red to purple and even to blue depending on the concentration of spermine, which could be developed as the colorimetric analysis of spermine. ctDNA-AuNPs assembly was demonstrated as a novel visual probe for the specific sensing of spermine with high specificity and sensitivity.

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