Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 120
Filtrar
1.
J Adv Nurs ; 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32153052

RESUMO

AIM: To identify the intention of Chinese pregnant women to undertake physical activity (PA) using the theory of planned behavior. DESIGN: A cross-sectional survey. METHODS: From April - October in 2017, a cross-sectional questionnaire was completed by 746 pregnant women from the Health Birth Cohort in Wuhan, China. The theory of planned behavior variables as well as sociodemographic characteristics was recorded, and the Pregnancy PA Questionnaire was together used to assess their PA during pregnancy. RESULTS: Only 11.3% of the women met the international guideline. The intention to undertake PA was found it to be positive in 63.9% of pregnant women. Structural equation modeling analysis revealed that behavioral attitudes, subjective norms and perceived behavioral control (PBC) influenced PA by directly influencing the behavior intention. Both behavioral attitude and subjective norms influenced PA by indirectly affecting the behavior. Overall, the model described 60% variance of the behavioral intention to undertake PA during pregnancy. CONCLUSION: PBC was confirmed to be a prominent factor in determining behavioral intention to undertake PA during pregnancy. Pregnant women should be helped and appropriately guided by health providers to overcome barriers to PA. EFFECT: This study investigates the effect of perceived behavioral control (PBC) on the intention to undertake physical activity (PA). The findings suggest that nurses' and midwives' attention should be focused on how to promote the improvement of perceived behavioral control ability of pregnant women to improve pregnant women's PA intention. The attitude of pregnant women on taking up PA and their ability to control behaviors can be improved with support from family or healthcare providers.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32003210

RESUMO

For the first time, we report the successful fabrication of well-behaved field-effect transistors based on Nb-doped ß-Ga2O3 nanobelts mechanically exfoliated from bulk single crystals. The exfoliated ß-Ga2O3 nanobelts were transferred onto a purified surface of the 110 nm SiO2/Si substrate. These Nb-doped devices showed excellent electrical performance such as an ultrasmall cutoff current of ∼10 fA, a high current on/off ratio of >108, and a quite steep subthreshold swing (SS, ∼120 mV/decade). Furthermore, we investigated the temperature dependence down to 200 K, providing insightful information for its operation in a harsh environment. This work lays a foundation for wider application of Nb-doped ß-Ga2O3 in nano-electronics.

3.
Chem Commun (Camb) ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040110

RESUMO

Here, a hollowed-out Au@AgPt core-frame nanostructure is carved in the presence of PtCl62- via galvanic replacement (GR) reaction, during which the dissolution of Ag atoms from the {100} facets and the deposition of Pt atoms on the active edges of the nanocubes occur. Both ex situ and in situ monitoring of the plasmonic and structural evolutions at the single-particle level, confirmed also by theoretical simulations, shows a three-phase mechanism involved.

4.
Anal Chem ; 92(5): 4046-4052, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32048509

RESUMO

Isothermal nucleic acid amplification technology has been widely adopted for analytical chemistry with the purpose of sensitivity improvement. Herein we present an ultrasensitive concatenated hybridization chain reaction (C-HCR) based surface-enhanced Raman scattering (SERS) immunoassay by forming antibody-antigen-aptamer heterosandwich structures with the model analyte of total prostate specific antigens (tPSA). In the C-HCR, two HCRs, one proceeds with two hairpins and the other with four biotin-modified hairpins, are coupled, making the formation of DNA nanofirecrackers with the lengths longer than 200 nm and more than four hundred million binding sites of streptavidin modified enzymes. These types of DNA nanofirecrackers through the aptamer encoded linker strand to form heterosandwich structures could provide a general signal application platform such as enzyme catalysis with high amplification efficiency. As a proof of concept, the Au@Ag core-shell nanostructure based SERS immunoassay with excellent signal amplification has been developed by employing the streptavidin modified alkaline phosphatase (SA-ALP) through its catalysis of 2-phospho-l-ascorbic acid trisodium salt (AAP) to form Au@Ag core-shell nanostructures via the formation of ascorbic acid (AA) to reduce AgNO3 and deposition of silver element on gold nanorods (AuNRs). The newly developed method has a detection limit as low as 0.94 fg/mL and has successfully achieved the detection of serum samples from clinical patients, which was consistent with the clinical test results, showing that this C-HCR strategy to form DNA nanofirecrackers has great potential in clinical applications.

5.
Int J Cancer ; 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31970767

RESUMO

Human papillomavirus (HPV) test, self-sampling and thermal ablation for cervical intraepithelial neoplasia (CIN) have been developed separately to increase screening coverage and treatment compliance of cervical cancer screening programmes. A large-scale study in rural China screened 9,526 women with their combinations to explore the optimal cervical cancer-screening cascade in the real-world. Participants received careHPV and polymerase chain reaction (PCR) HPV tests on self-collected samples. Women positive on either HPV test underwent colposcopy, biopsy and thermal ablation in a single visit. Samples positive on either HPV test were retested for genotyping. Absolute and relative performance of HPV tests, triage strategies, 'colposcopy and thermal ablation' approach were statistically evaluated. PCR HPV test detected 33.3% more CIN grade two or worse (CIN2+) at a cost of 28.1% more colposcopies compared to careHPV. Sensitivities of PCR HPV and careHPV tests to detect CIN2+ were 96.7 and 72.5%. Specificities for the same disease outcome were 82.1 and 86.0%. Triaging HPV-positive women with HPV16/18 genotyping considerably improved the positive predictive value for CIN2+ (4.8-5.0 to 18.2-19.2%). Ninety-six women positive on HPV and having abnormal colposcopy were eligible for thermal ablation and all accepted same-day treatment, contributing to 64.6% being treated appropriately (CIN1+ on histopathology), which reached up to 84.8% among women positive on HPV 16/18 triage. No serious side-effects/complications were reported. The combination of PCR HPV test followed by HPV 16/18 triaging on self-collected samples and colposcopy of triage positive women followed by immediate thermal ablation might be the appropriate screening cascade for rural China.

6.
Anal Chem ; 92(2): 2130-2135, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31850751

RESUMO

Plasmon resonance energy transfer (PRET), as a new form of energy transfer first discovered in 2007, has been widely applied for the biomolecular recognition, detection of ions, cellular physiological status monitoring, and energy conversion. It occurs between noble metal nanoparticles (donor) and conjugated molecules or nanoparticles (acceptor). In this study, we used urchin-like gold nanoplasmonics (UGPs) and oxTMB as a new donor-acceptor pair to establish a novel PRET coupling system, avoiding trivial modification. PRET from UGPs to conjugated redox-active oxTMB leads to resonant quenching in the localized surface plasmon resonance (LSPR) spectra. However, when the acid phosphatase (ACP) was introduced, the hydrolyzate ascorbic acid (AA) converted from 2-phospho-l-ascorbic acid trisodium salt (AAP) could be capable of reducing oxTMB into TMB, thereby preventing the occurrence of PRET. The recovery of the scattering spectral intensity of UGPs was linearly related to the concentration of ACP in the range of 0.1 to 5.0 U/L, and the ACP with a detection limit of 0.076 U/L could be measured. In addition, this method also showed good selectivity attributed to the substrate specificity of enzyme.

7.
Nanoscale Res Lett ; 14(1): 360, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792627

RESUMO

Hybrid heterojunctions based on two-dimensional (2D) and conventional three-dimensional (3D) materials provide a promising way toward nanoelectronic devices with engineered features. In this work, we investigated the band alignment of a mixed-dimensional heterojunction composed of transferred MoS2 on ß-Ga2O3([Formula: see text]01) with and without nitridation. The conduction and valence band offsets for unnitrided 2D-MoS2/3D-ß-Ga2O3 heterojunction were determined to be respectively 0.43 ± 0.1 and 2.87 ± 0.1 eV. For the nitrided heterojunction, the conduction and valence band offsets were deduced to 0.68 ± 0.1 and 2.62 ± 0.1 eV, respectively. The modified band alignment could result from the dipole formed by charge transfer across the heterojunction interface. The effect of nitridation on the band alignments between group III oxides and transition metal dichalcogenides will supply feasible technical routes for designing their heterojunction-based electronic and optoelectronic devices.

8.
Int J Obes (Lond) ; 43(12): 2434-2447, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31040394

RESUMO

OBJECTIVE: Myeloid-related protein-14 (MRP14) and its binding partner MRP8 play an essential role in innate immune function and have been implicated in a variety of inflammatory diseases. However, the role of MRP14 in obesity-induced inflammation and insulin resistance is not well defined. This study investigated the role of MRP14 in macrophage-mediated adipose tissue inflammation and obesity-induced insulin resistance. SUBJECTS AND RESULTS: Wild-type (WT) and Mrp14-/- mice were fed with a high-fat diet or normal chow for 12 weeks. Tissue-resident macrophages in both adipose tissue and liver from obese WT mice expressed higher levels of MRP14 in the visceral adipose fat and liver compared with the lean mice. Mrp14-/- mice demonstrated a significantly improved postprandial insulin sensitivity, as measured by intraperitoneal glucose tolerance test and insulin tolerance testing. Macrophages secreted MRP14 in response to inflammatory stimuli, such as LPS. Extracellular MRP8/14 induced the production of CCL5 and CXCL9. Deficiency of MRP14 did not affect macrophage proliferation, mitochondrial respiration, and glycolytic function, but Mrp14-/- macrophages showed a reduced ability to attract T cells. Depletion of the extracellular MRP14 reduced the T cell attracting ability of WT macrophages to a level similar to Mrp14-/- macrophages. CONCLUSION: Our data indicate that MRP14 deficiency decreases obesity-induced insulin resistance and MRP8/14 regulates T-cell recruitment through the induction of T-cell chemoattractant production from macrophages.

9.
Toxicol Sci ; 169(1): 95-107, 2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-30812033

RESUMO

Fine ambient particulate matter (PM2.5) is able to induce sympathetic activation and inflammation in the brain. However, direct evidence demonstrating an essential role of sympathetic activation in PM2.5-associated disease progression is lacking. We assess the contribution of α2B-adrenergic receptor (Adra2b) in air pollution-associated hypertension and behavioral changes in this study. Wild-type mice and Adra2b-transgenic mice overexpressing Adra2b in the brain (Adra2bTg) were exposed to concentrated PM2.5 or filtered air for 3 months via a versatile aerosol concentrator exposure system. Mice were fed with a high salt diet (4.0% NaCl) for 1 week at week 11 of exposure to induce blood pressure elevation. Intra-arterial blood pressure was monitored by radio-telemetry and behavior changes were assessed by open field, light-dark, and prepulse inhibition tests. PM2.5 exposure increased Adra2b in the brain of wild-type mice. Adra2b overexpression enhanced the anxiety-like behavior and high salt diet-induced blood pressure elevation in response to air pollution but not filtered air exposure. Adra2b overexpression induced upregulation of inflammatory genes such as TLR2, TLR4, and IL-6 in the brain exposed to PM2.5. In addition, there were increased frequencies of activated effector T cells and increased expression of oxidative stress-related genes, such as SOD1, NQO1, Nrf2, and Gclm in Adra2bTg mice compared with wild-type mice. Our results provide new evidence of distinct behavioral changes consistent with anxiety and blood pressure elevation in response to high salt intake and air pollution exposure, highlighting the importance of centrally expressed Adra2b in the vulnerability to air pollution exposure.

10.
Biol Trace Elem Res ; 189(1): 180-185, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30069692

RESUMO

Zinc (Zn) highly concentrates in the brain and plays a key role in memory formation and learning processes. Zn deficiency results in cognitive impairments, memory deficits, alterations of neuropsychological behavior, and motor development. Although Zn-enriched yeast (ZnY) is widely used for dietary fortification and supplementation of Zn, the effect of ZnY on cognition still remains unclear. The purpose of the study was to investigate the effect of ZnY on behavior in Zn-deficient and Zn-sufficient rats. Three-week-old rats were fed low Zn diets for 145 days to establish Zn-deficient rats. ZnY was orally administered to Zn-deficient rats at three dose levels of 1, 2, and 4 mg Zn/kg/day for 55 days. Rat appearance, body weight, plasma and brain Zn, Morris water maze test, and step-through passive avoidance test were observed. Compared to Zn-sufficient rats, body weight gain, plasma zinc level, resident time, and step-through time in Zn-deficient rats were significantly lower. Zn deficiency impaired functions of learning and memory, while ZnY as a plausible therapeutic intervention alleviated the cognitive impairments caused by Zn deficiency.


Assuntos
Fermento Seco , Zinco/farmacologia , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Suplementos Nutricionais , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Transtornos da Memória/sangue , Transtornos da Memória/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Zinco/sangue
11.
Micromachines (Basel) ; 9(2)2018 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-30393350

RESUMO

A three-dimensional topography simulation of deep reactive ion etching (DRIE) is developed based on the narrow band level set method for surface evolution and Monte Carlo method for flux distribution. The advanced level set method is implemented to simulate the time-related movements of etched surface. In the meanwhile, accelerated by ray tracing algorithm, the Monte Carlo method incorporates all dominant physical and chemical mechanisms such as ion-enhanced etching, ballistic transport, ion scattering, and sidewall passivation. The modified models of charged particles and neutral particles are epitomized to determine the contributions of etching rate. The effects such as scalloping effect and lag effect are investigated in simulations and experiments. Besides, the quantitative analyses are conducted to measure the simulation error. Finally, this simulator will be served as an accurate prediction tool for some MEMS fabrications.

12.
J Infect Chemother ; 24(11): 887-891, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30197093

RESUMO

The accuracy of the test is critical for the syphilis serology diagnosis. This study aims to evaluate the values of the Elecsys syphilis assay, the Architect syphilis assay, and the Mindray syphilis assay, as syphilis screening tests for pregnant women and patients with syphilis or other diseases. A reverse algorithm was used for the syphilis serology diagnosis. Serum samples (n = 584) were tested with three automated screening assays. All reactive sera by one, two, or three screening assays were further analyzed with the tolulized red unheated serum test (TRUST). Inconsistent results were confirmed by the Treponema pallidum particle agglutination assay (TPPA). The final patient diagnosis was made according to the results of syphilis serology, clinical evidence, and past medical history. The sensitivity, specificity, accuracy, and kappa value of each assay were as follows: for the Elecsys syphilis assay, 100.0%, 98.5%, 98.6%, and 0.927, respectively; for the Architect syphilis assay: 100.0%, 94.5%, 95.0%, and 0.770; and for the Mindray syphilis assay: 100.0%, 97.0%, 97.3%, and 0.862. The McNemar test showed that there were significant differences in the performance between the Elecsys syphilis assay and the Architect syphilis assay (P < 0.001), and between the Mindray syphilis assay and the Architect syphilis assay (P = 0.001). Our study demonstrated that three automated Treponema pallidum antibody assays generally showed high sensitivities and specificities, and so, they are suitable for use in screening for syphilis. The performances of the Elecsys syphilis assay and the Mindray syphilis assay are superior to Architect syphilis assay.


Assuntos
Anticorpos Antibacterianos/isolamento & purificação , Complicações Infecciosas na Gravidez/diagnóstico , Sorodiagnóstico da Sífilis/métodos , Sífilis/diagnóstico , Treponema pallidum/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Algoritmos , Anticorpos Antibacterianos/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Gravidez , Complicações Infecciosas na Gravidez/sangue , Complicações Infecciosas na Gravidez/imunologia , Complicações Infecciosas na Gravidez/microbiologia , Estudos Prospectivos , Sensibilidade e Especificidade , Sífilis/sangue , Sífilis/imunologia , Sífilis/microbiologia , Treponema pallidum/isolamento & purificação , Adulto Jovem
13.
Nanoscale Res Lett ; 13(1): 246, 2018 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-30136254

RESUMO

Ultra-wide bandgap beta-gallium oxide (ß-Ga2O3) has been attracting considerable attention as a promising semiconductor material for next-generation power electronics. It possesses excellent material properties such as a wide bandgap of 4.6-4.9 eV, a high breakdown electric field of 8 MV/cm, and exceptional Baliga's figure of merit (BFOM), along with superior chemical and thermal stability. These features suggest its great potential for future applications in power and optoelectronic devices. However, the critical issue of contacts between metal and Ga2O3 limits the performance of ß-Ga2O3 devices. In this work, we have reviewed the advances on contacts of ß-Ga2O3 MOSFETs. For improving contact properties, four main approaches are summarized and analyzed in details, including pre-treatment, post-treatment, multilayer metal electrode, and introducing an interlayer. By comparison, the latter two methods are being studied intensively and more favorable than the pre-treatment which would inevitably generate uncontrollable damages. Finally, conclusions and future perspectives for improving Ohmic contacts further are presented.

14.
J Clin Lab Anal ; 32(9): e22600, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30058207

RESUMO

BACKGROUND: Anti-hepatitis C virus (anti-HCV) antibody assays are recommended for HCV infection screening. The Mindray anti-HCV assay, based on a third-generation immunoassay, was recently launched in China. We aimed to evaluate its diagnostic performance compared with that of two other widely used assays. METHODS: Six HCV infection seroconversion panels were used to evaluate the sensitivity of the assay for early detection. A total of 1952 clinical samples were tested by the Mindray anti-HCV, Elecsys anti-HCV II, and Architect anti-HCV assays. Samples with reactive results using at least one anti-HCV assay were further tested with the recombinant immunoblot assay (RIBA). Inconsistent results were investigated by the HCV RNA assay and HCV core antigen assay. HCV infection diagnosis was made according to the results of laboratory tests and medical records. RESULTS: The Mindray anti-HCV assay and Elecsys anti-HCV II assay detected seroconversion in an average of 12.5 days and 10.5 days, respectively, and this difference was not significant (P = .818). Of the 1952 cases, 90 were categorized as "HCV infection" and 1862 were categorized as "no HCV infection." The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (LR+), and negative likelihood ratio (LR-) of each assay were as follows: the Mindray anti-HCV assay, 95.6%, 99.2%, 85.1%, 99.8%, 118.6 and 0.045, respectively; the Architect anti-HCV assay, 98.9%, 95.2%, 50.0%, 99.9%, 20.69 and 0.012, respectively; and the Elecsys anti-HCV II assay, 96.7%, 99.9%, 98.9%, 99.8%, 1799.9 and 0.033, respectively. There were significant differences in the specificity, PPV and LR+ among the three assays (P < .001). There were no significant differences in the sensitivity, NPV or LR- among the three assays (P > .05). CONCLUSIONS: The Mindray anti-HCV assay displays a similar sensitivity to the Elecsys anti-HCV II assay with respect to the early detection of HCV infection. The Mindray anti-HCV assay shows excellent diagnostic performance and is suitable for the screening of HCV infection.


Assuntos
Anticorpos Anti-Hepatite C/sangue , Hepatite C/diagnóstico , Hepatite C/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Hepatite C/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Sensibilidade e Especificidade , Soroconversão/fisiologia , Adulto Jovem
15.
Clin Immunol ; 195: 139-148, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29894743

RESUMO

The major metabolic feature of diabetes is hyperglycemia which has been linked to the diabetes inflammatory processes, and diabetes-related vulnerability to infection. In the present study, we assessed how glucose affected PBMCs in type I interferon (IFN) production and subsequent signaling. We found that the moderately elevated glucose promoted, and high glucose suppressed type I IFN production, respectively. Pre-exposure to high glucose rendered monocytes more sensitive to IFN-α stimulation with heightened signaling, whereas, instantaneous addition of high glucose did not exhibit such effect. Consistent with this finding, the mRNA levels of IFN-α-induced IRF-7 in PBMCs were positively correlated with HbA1c levels of diabetes patients. Additionally, we found that high glucose promoted the production of other proinflammatory cytokines/chemokines. This study suggests that hyperglycemia may affect the inflammatory process in diabetes via promoting proinflammatory cytokines, as well as the host defense against microbial infections through impeding type I IFN production and signaling.


Assuntos
Complicações do Diabetes/imunologia , Glucose/metabolismo , Hiperglicemia/imunologia , Inflamação/imunologia , Interferon Tipo I/metabolismo , Monócitos/imunologia , Adolescente , Adulto , Criança , Feminino , Hemoglobina A Glicada/metabolismo , Humanos , Imunidade , Fator Regulador 7 de Interferon/genética , Fator Regulador 7 de Interferon/metabolismo , Masculino , Lectina 1 Semelhante a Ig de Ligação ao Ácido Siálico/metabolismo , Transdução de Sinais , Células THP-1 , Adulto Jovem
16.
Artigo em Inglês | MEDLINE | ID: mdl-29735559

RESUMO

Muraymycins are antibacterial natural products from Streptomyces spp. that inhibit translocase I (MraY), which is involved in cell wall biosynthesis. Structurally, muraymycins consist of a 5'-C-glycyluridine (GlyU) appended to a 5″-amino-5″-deoxyribose (ADR), forming a disaccharide core that is found in several peptidyl nucleoside inhibitors of MraY. For muraymycins, the GlyU-ADR disaccharide is further modified with an aminopropyl-linked peptide to generate the simplest structures, annotated as the muraymycin D series. Two enzymes encoded in the muraymycin biosynthetic gene cluster, Mur29 and Mur28, were functionally assigned in vitro as a Mg·ATP-dependent nucleotidyltransferase and a Mg·ATP-dependent phosphotransferase, respectively, both modifying the 3″-OH of the disaccharide. Biochemical characterization revealed that both enzymes can utilize several nucleotide donors as cosubstrates and the acceptor substrate muraymycin also behaves as an inhibitor. Single-substrate kinetic analyses revealed that Mur28 preferentially phosphorylates a synthetic GlyU-ADR disaccharide, a hypothetical biosynthetic precursor of muraymycins, while Mur29 preferentially adenylates the D series of muraymycins. The adenylated or phosphorylated products have significantly reduced (170-fold and 51-fold, respectively) MraY inhibitory activities and reduced antibacterial activities, compared with the respective unmodified muraymycins. The results are consistent with Mur29-catalyzed adenylation and Mur28-catalyzed phosphorylation serving as complementary self-resistance mechanisms, with a distinct temporal order during muraymycin biosynthesis.


Assuntos
Proteínas de Bactérias/antagonistas & inibidores , Nucleosídeos/biossíntese , Nucleosídeos/química , Nucleotidiltransferases/química , Peptídeos/química , Fosfotransferases/química , Streptomyces/metabolismo , Transferases/antagonistas & inibidores , Antibacterianos/biossíntese , Nucleotídeos/biossíntese , Nucleotidiltransferases/genética , Fosforilação , Fosfotransferases/genética
17.
J Immunol Res ; 2018: 5463879, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29651443

RESUMO

Dendritic cell (DC) immunotherapy has been effective for prevention of type 1 diabetes (T1D) in NOD mice but fails to protect if initiated after active autoimmunity. As autoreactivity expands inter- and intramolecularly during disease progression, we investigated whether DCs unpulsed or pulsed with ß cell antigenic dominant determinants (DD), subdominant determinants (SD), and ignored determinants (ID) could prevent T1D in mice with advanced insulitis. We found that diabetes was significantly delayed by DC therapy. Of interest, DCs pulsed with SD or ID appeared to provide better protection. T lymphocytes from DC-treated mice acquired spontaneous proliferating capability during in vitro culture, which could be largely eliminated by IL-2 neutralizing antibodies. This trend maintained even 29 weeks after discontinuing DC therapy and appeared antigen-independent. Furthermore, CD4+Foxp3+ T regulatory cells (Tregs) from DC-treated mice proliferated more actively in vitro compared to the controls, and Tregs from DC-treated mice showed significantly enhanced immunosuppressive activities in contrast to those from the controls. Our study demonstrates that DC therapy leads to long-lasting immunomodulatory effects in an antigen-dependent and antigen-independent manner and provides evidence for peptide-based intervention during a clinically relevant window to guide DC-based immunotherapy for autoimmune diabetes.


Assuntos
Células Dendríticas/fisiologia , Diabetes Mellitus Tipo 1/terapia , Imunoterapia Adotiva/métodos , Subpopulações de Linfócitos T/imunologia , Linfócitos T Reguladores/imunologia , Animais , Apresentação do Antígeno , Autoantígenos/imunologia , Autoantígenos/metabolismo , Autoimunidade , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Diabetes Mellitus Tipo 1/imunologia , Modelos Animais de Doenças , Epitopos de Linfócito T/imunologia , Epitopos de Linfócito T/metabolismo , Feminino , Fatores de Transcrição Forkhead/metabolismo , Humanos , Tolerância Imunológica , Epitopos Imunodominantes/imunologia , Epitopos Imunodominantes/metabolismo , Imunomodulação , Células Secretoras de Insulina/imunologia , Células Secretoras de Insulina/metabolismo , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos NOD
18.
Phys Chem Chem Phys ; 20(9): 6688-6697, 2018 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-29457164

RESUMO

To broaden the absorption spectrum, improve intramolecular electronic push-pull balance capability, enhance electron transfer and promote the photovoltaic performances of dye sensitizers, four new polymeric metal complex dyes (PBDTT-PhenCd, PBDTT-PhenCu, PPV-PhenCd and PPV-PhenCu) with donor-acceptor-π-bridge-acceptor (D-A-π-A) structure were designed and synthesized. These dyes, for the first time, used the complexes of Cd(ii) or Cu(ii) with phenanthroline as auxiliary electron acceptors (A) instead of organic electron withdrawing groups and adopted thienylbenzo [1,2-b:4,5-b'] dithiophene (BDTT) or hydroquinone (PV) derivatives as electron donors (D) and 8-quinolinol derivatives as π bridge and acceptors (A). The impacts of different auxiliary electron acceptors (A) of metal complexes due to their thermal, optical, electrochemical properties and photovoltaic performance were also investigated. The best power conversion efficiency of 6.68% was achieved in PBDTT-PhenCd DSSC device. In addition, all dyes showed outstanding thermal stability (Td > 300 °C). The results revealed that these novel polymeric metal complex dyes are potential materials for constructing new sensitizers of high performance.

19.
Mediators Inflamm ; 2018: 4316584, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30670926

RESUMO

Myeloid-derived suppressor cells (MDSCs) are identified as a heterogeneous population of cells with the function to suppress innate as well as adaptive immune responses. The initial studies of MDSCs were primarily focused on the field of animal tumor models or cancer patients. In cancer, MDSCs play the deleterious role to inhibit tumor immunity and to promote tumor development. Over the past few years, an increasing number of studies have investigated the role of MDSCs in autoimmune diseases. The beneficial effects of MDSCs in autoimmunity have been reported by some studies, and thus, immunosuppressive MDSCs may be a novel therapeutic target in autoimmune diseases. There are some controversial findings as well. Many questions such as the activation, differentiation, and suppressive functions of MDSCs and their roles in autoimmune diseases remain unclear. In this review, we have discussed the current understanding of MDSCs in autoimmune diseases.


Assuntos
Doenças Autoimunes/imunologia , Doenças Autoimunes/terapia , Células Supressoras Mieloides/imunologia , Animais , Autoimunidade/fisiologia , Humanos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA