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1.
Int J Phytoremediation ; : 1-6, 2020 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-33197328

RESUMO

The study aimed at accessing the effects of mowing on the growth and cadmium (Cd) accumulation of the first post-grafting generation of potential Cd-hyperaccumulator plant Solanum photeinocarpum through the pot and plot experiments. Four grafting treatments were employed such as ungrafted (UG), self-rooted grafting by the same S. photeinocarpum seedling (SG), self-rooted grafting by two different development stages of S. photeinocarpum seedlings (DG), and grafting on the rootstock of wild potato (PG). The biomass, Cd content, and Cd extraction amount of S. photeinocarpum shoots significantly decreased with the increase of mowing times in the pot and plot experiments. The order of the grafting on the biomass, Cd content, and Cd extraction amountof in the first, second, third mowing shoots were PG > DG > SG > UG. For the Cd extraction amountof total moving shoots, SG, DG, and PG increased by 20.42%, 35.54%, and 52.94%, respectively, in the pot experiment, and increased by 11.56%, 26.28%, and 44.90%, respectively, in the plot experiment, compared with the UG. Therefore, mowing provides an insight into the phytoremediation ability of S. photeinocarpum to Cd.

2.
BMC Genomics ; 21(1): 776, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33167867

RESUMO

BACKGROUND: Drought-tolerance ensures a crop to maintain life activities and protect cell from damages under dehydration. It refers to diverse mechanisms temporally activated when the crop adapts to drought. However, knowledge about the temporal dynamics of rice transcriptome under drought is limited. RESULTS: Here, we investigated temporal transcriptomic dynamics in 12 rice genotypes, which varied in drought tolerance (DT), under a naturally occurred drought in fields. The tolerant genotypes possess less differentially expressed genes (DEGs) while they have higher proportions of upregulated DEGs. Tolerant and susceptible genotypes have great differences in temporally activated biological processes (BPs) during the drought period and at the recovery stage based on their DEGs. The DT-featured BPs, which are activated specially (e.g. raffinose, fucose, and trehalose metabolic processes, etc.) or earlier in the tolerant genotypes (e.g. protein and histone deacetylation, protein peptidyl-prolyl isomerization, transcriptional attenuation, ferric iron transport, etc.) shall contribute to DT. Meanwhile, the tolerant genotypes and the susceptible genotypes also present great differences in photosynthesis and cross-talks among phytohormones under drought. A certain transcriptomic tradeoff between DT and productivity is observed. Tolerant genotypes have a better balance between DT and productivity under drought by activating drought-responsive genes appropriately. Twenty hub genes in the gene coexpression network, which are correlated with DT but without potential penalties in productivity, are recommended as good candidates for DT. CONCLUSIONS: Findings of this study provide us informative cues about rice temporal transcriptomic dynamics under drought and strengthen our system-level understandings in rice DT.

3.
Small ; : e2006366, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33230931

RESUMO

Sodium-ion batteries (SIBs) have been considered as one of the most promising secondary battery techniques for large-scale energy storage applications. However, developing appropriate electrode materials that can satisfy the demands of long-term cycling and high energy/power capabilities remains a challenge. Herein, a fluorine modulation strategy is reported that can trigger highly active exposed crystal facets in anatase TiO2- x Fx , while simultaneously inducing improved electron transfer and Na+ diffusion via lattice regulation. When tested in SIBs, the optimized fluorine doped TiO2- x Fx nanocrystals exhibit a high reversible capacity of 275 mA h g-1 at 0.05 A g-1 , outstanding rate capability (delivering 129 mA h g-1 at 10 A g-1 ), and remarkable cycling stability with 91% capacity retained after 6000 cycles at 2 A g-1 . Importantly, the optimized TiO2- x Fx nanocrystals are dominated by pseudocapacitive Na+ storage, which can be attributed to the fluorine induced surface and lattice regulation, enabling ultrafast electrode kinetics.

4.
Phys Med Biol ; 65(21): 215024, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33151907

RESUMO

Magneto-acousto-electrical tomography (MAET) is an imaging method coupled with sound field and magnetic field. The aim of this study is to present some novel experimental results of the mouse liver for the magneto-acousto-electrical tomography measured by two electrodes. The magnetic field in the space of 60 mm3 is about 300 mT which generate by two permanent magnets. A plane transducer with 2.25 MHz center frequency is utilized to generate acoustic waves inside the object. The signal is detected by two similar 1 mm copper foil electrodes. An amplifier is designed to receive the MAET signal, and the gain of the amplifier is adjusted to be 54 dB. The phantom used in this paper is a mouse liver surrounded by a gel phantom with the conductivity of 0.7 S m-1. The gel phantom with the conductivity of 0.7 S m-1 is used to simulate the liver tumor, and the normal mouse liver is filled in the phantom. A series of the MAET signals are detected by the electrodes when the transducer is moved on a pre-set line route, then a B-scan image is realized. The experimental system can provide more information about the tumor and the results show that the MAET is sensitive enough for the potential clinical application of tumor in animal or human.

5.
Bioorg Chem ; 104: 104257, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32927129

RESUMO

BACKGROUND: Oseltamivir is a first-line antiviral drug, especially in primary hospitals. During the ongoing outbreak of coronavirus disease 2019 (COVID-19), most patients with COVID-19 who are symptomatic have used oseltamivir. Considering its popular and important role as an antiviral drug, it is necessary to evaluate oseltamivir in the treatment of COVID-19. OBJECTIVE: To evaluate the effect of oseltamivir against COVID-19. METHODS: Swiss-model was used to construct the structure of the N-terminal RNA-binding domain (NRBD) of the nucleoprotein (NC), papain-like protease (PLpro), and RNA-directed RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). TM-align program was performed to compare the structure of the viral proteins with the structure of the neuraminidase of influenza A. Molecular docking was used to analyze the theoretical possibility of effective binding of oseltamivir with the active centers of the viral proteins. In vitro study was used to evaluate the antiviral efficiency of oseltamivir against SARS-CoV-2. By clinical case analysis, we statistically evaluated whether the history of oseltamivir use influenced the progression of the disease. RESULTS: The structures of NRBD, PLpro, and RdRp were built successfully. The results from TM-align suggested that the S protein, NRBD, 3C-like protease (3CLpro), PLPrO, and RdRp were structurally similar to the influenza A neuraminidase, with TM-scores of 0.30077, 0.19254, 0.28766, 0.30666, and 0.34047, respectively. Interestingly, the active center of 3CL pro was found to be similar to the active center from the neuraminidase of influenza A. Through an analysis of molecular docking, we discovered that oseltamivir carboxylic acid was more favorable to bind to the active site of 3CLpro effectively, but its inhibitory effect was not strong compared with the positive group. Finally, we used in vitro study and retrospective case analysis to verify our speculations. We found that oseltamivir is ineffective against SARS-CoV-2 in vitro study and the clinical use of oseltamivir did not improve the patients' symptoms and signs and did not slow the disease progression. CONCLUSIONS: We consider that oseltamivir isn't suitable for the treatment of COVID-19. During the outbreak of novel coronavirus, when oseltamivir is not effective for the patients after they take it, health workers should be highly vigilant about the possibility of COVID-19.

6.
Am J Hypertens ; 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32886761

RESUMO

BACKGROUND: There is a lack of studies comparing hypertension and comorbidities in rural and urban older people in China. This study aimed to investigate the prevalence of hypertension and `comorbidities in older people, and to describe differences between patients from rural and urban areas in China. METHODS: A cross-sectional study on global AGEing and adult health (SAGE) was conducted from 2007 to 2010 across eight provinces in China. The current study included a nationally representative sub-sample of 7403 respondents aged 60 years and above. Hypertension was either self-reported diagnosed hypertension or one elevated measured blood pressure measurement (≥ 140/90 mmHg). Comorbidities were self-reported and included angina, arthritis, asthma, stroke, diabetes, depression and chronic lung disease. RESULTS: The prevalence of hypertension was 66.1%. Hypertension associated with one comorbidity was recorded in 35.9% of all participants. A large majority (73.6%) of participants with hypertension reported "good" or "very good" general health status. The prevalence of hypertension and the number of co-morbidities increased with age. The prevalence rates of multiple co-morbidities were higher for urban participants than their rural counterparts (27.8 vs 42.4% for 1 morbidity; 7.7 vs 16.4% for 2 comorbidities; 1.4 vs 4.7% for 3 comorbidities; 0.4 vs 1.2% for ≥4 comorbidities) after adjustment for socio-demographic factors. However, urban participants reported better general health status, irrespective of the number of comorbidities. CONCLUSIONS: Our study implies that health promotion policies targeting older people should be developed. Rural areas should be a priority, and investment in the quality of healthcare in rural areas is urgently needed.

7.
J Agric Food Chem ; 68(41): 11421-11433, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-32936614

RESUMO

The newly released Donghong kiwifruit is a promising commercial cultivar. The dynamic changes of major phenolic compounds (flavonols, flavanols, phenolic acids, and anthocyanins) during the representative stages of fruit development and ripening of the Donghong kiwifruit were determined by high-performance liquid chromatography. The corresponding time-course transcriptional changes were evaluated using the combined analysis of RNA-Seq and qRT-PCR. The most predominant phenolic compound in the Donghong kiwifruit was epicatechin. Cyanidin 3-O-[2-O-(ß-xylosyl)-ß-galactoside] and cyanidin 3-O-ß-galactoside were two essential anthocyanins detected. Candidate genes and pathways involved in phenolic compounds biosynthesis were highlighted. The structural genes (AcLDOX2, Ac5GGT1, and Ac5AT2) and the transcription factor (bHLH74-2) were strongly associated with anthocyanin biosynthesis. AcMYB4-1 may be a novel transcription factor that reduces anthocyanin accumulation. Results from the study may be a very useful supplement to current knowledge of molecular mechanisms to elucidate coloration in the red-fleshed kiwifruit and could help breeders modify the kiwifruit germplasm.

8.
Artigo em Inglês | MEDLINE | ID: mdl-32954557

RESUMO

Owing to the limited charge storage capability of transitional metal oxides in aqueous electrolytes, the use of redox electrolytes (RE) represents a promising strategy to further increase the energy density of aqueous batteries or pseudocapacitors. The usual coupling of an electrode and an RE possesses weak electrode/RE interaction and weak adsorption of redox moieties on the electrode, resulting in a low capacity contribution and fast self-discharge. In this work, Fe(CN)6 4- groups are grafted on the surface of Co3 O4 electrode via formation of CoN bonds, creating a synergistic interface between the electrode and the RE. With such an interface, the coupled Co3 O4 -RE system exhibits greatly enhanced charge storage from both Co3 O4 and RE, delivering a large reversible capacity of ≈1000 mC cm-2 together with greatly reduced self-discharge. The significantly improved electrochemical activity of Co3 O4 can be attributed to the tuned work function via charge injection from Fe(CN)6 4- , while the greatly enhanced adsorption of K3 Fe(CN)6 molecules is achieved by the interface induced dipole-dipole interaction on the liquid side. Furthermore, this enhanced electrode-electrolyte coupling is also applicable in the NiO-RE system, demonstrating that the synergistic interface design can be a general strategy to integrate electrode and electrolyte for high-performance energy storage devices.

9.
EBioMedicine ; 58: 102924, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32739872

RESUMO

BACKGROUND: This study aimed to establish and validate a novel scoring system based on a nomogram for the differential diagnosis of malignant pleural effusion (MPE) and benign pleural effusion (BPE). METHODS: Patients with PE and confirmed aetiology who underwent diagnostic thoracentesis were included in this study. One retrospective set (N = 1261) was used to develop and internally validate the predictive model. The clinical, radiological and laboratory features were collected and subjected to logistic regression analyses. The primary predictive model was displayed as a nomogram and then modified into a novel scoring system, which was externally validated in an independent set (N = 172). FINDINGS: The novel scoring system was composed of fever (3 points), erythrocyte sedimentation rate (4 points), effusion adenosine deaminase (7 points), serum carcinoembryonic antigen (CEA) (4 points), effusion CEA (10 points) and effusion/serum CEA (8 points). With a cutoff value of 15 points, the area under the curve, specificity and sensitivity for identifying MPE were 0.913, 89.10%, and 82.63%, respectively, in the training set, 0.922, 93.48%, 81.51%, respectively, in the internal validation set and 0.912, 87.61%, 81.36%, respectively, in the external validation set. Moreover, this scoring system was exclusively applied to distinguish lung cancer with PE from tuberculous pleurisy and showed a favourable diagnostic performance in the training and validation sets. INTERPRETATION: This novel scoring system was developed from a retrospective study and externally validated in an independent set based on six easily accessible clinical variables, and it exhibited good diagnostic performance for identifying MPE. FUNDING: NFSC grants (no. 81572942, no. 81800094).

10.
Ecotoxicol Environ Saf ; 205: 111172, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32846300

RESUMO

Following oil extraction in the wetland of the Yellow River Delta, heavy metal contamination of coastal saline-alkaline soil, especially with cadmium (Cd), has become a serious environmental problem in some regions. Biochar application has been proposed to remedy Cd-contaminated soil, but the remediation effect is related to preparation conditions of biochar (e.g., pyrolysis temperature and raw material) and soil properties. The invasive plant, Spartina alterniflora, produces a high amount of biomass, making it suitable for biochar production in coastal China. We investigated the effect of S. alterniflora-derived biochar (SDB) pyrolyzed at four temperatures (350, 450, 550, and 650 °C) crossed with three addition ratios (1, 5, and 10%) and control on Cd contamination of coastal saline-alkaline soil. Pyrolysis temperature affected pH, surface area, and functional groups of SDB. SDB markedly improved soil pH and soil organic matter, but the degree of improvement was affected by pyrolysis temperature and addition ratio. SDB significantly altered available Cd content in soil, but reduced it only at low pyrolysis temperatures (350 and 450 °C). Available Cd content had a positive correlation with soil pH (R2 = 0.298, P < 0.01), but was not related to salinity and soil organic matter content. Thus, SDB pyrolyzed at 350 °C with 5% addition was optimal for passivating Cd in coastal saline-alkaline soil, since available Cd content in soil decreased mostly (by 26.9%). These findings act as a reference for the development of an application strategy for SDB to ameliorate Cd-contaminated coastal saline-alkaline soil.


Assuntos
Cádmio/análise , Carvão Vegetal/química , Recuperação e Remediação Ambiental/métodos , Poaceae/química , Poluentes do Solo/análise , Solo/química , Álcalis/análise , Biomassa , China , Modelos Teóricos , Pirólise , Salinidade , Áreas Alagadas
11.
Reprod Biol Endocrinol ; 18(1): 70, 2020 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-32664979

RESUMO

BACKGROUND: Although varicocele is considered to be one of the leading causes of male infertility, the precise mechanism underlying how varicocele leads to male infertility is not completely understood. We found the lactate concentration on the varicocele side of the patients was decreased compare with peripheral venous blood. In the testicles, the lactate produced by the sertoli cells through the glycolysis pathway provides most of the energy needed for spermatogenesis, the reduction of lactate will affect spermatogenesis. The objective of this study was to investigate the mechanism of this abnormal energy metabolism phenomenon in varicocele. METHODS: In this study, we collected the testicular tissue from patients with varicocele, the glycolysis related proteins PHGDH was identified by iTRAQ proteomics technology. Experimental rat varicocele model was constructed according to our new clip technique, the mRNA and protein expression levels of PHGDH were examined with qRT-PCR and Western blotting. We constructed a sertoli cell of PHGDH down-regulation model, and then detected the glucose consumption, LDH activities and lactate production in the sertoli cells. Western blot was conducted to investigate the effects of PHGDH on the expression of phosphoserine phosphatase (PSPH) and Pyruvate kinase M2 (PKM2). Flow cytometry was used to detect the cell apoptosis and cell cycle in sertoli cells. RESULTS: The results showed that testicular protein PHGDH was down-regulated in patients with varicocele and in experimental rat varicocele model. Down-regulation of PHGDH in sertoli cells significantly decreased the glucose consumption, LDH activities and lactate production in the sertoli cells, indicating that the low expression of PHGDH ultimately led to a decrease in lactate production by affecting the glycolysis. The Western blot results showed that the down-regulation of PHGDH significantly reduced the expression of pathway protein PSPH and PKM2, leading to the reduction of lactate production. Moreover, PHGDH knockdown can promote apoptosis and inhibit cell cycle to affect cell growth. CONCLUSIONS: Overall, we conformed that varicocele lead to the decreasing of testis lactate production. Down-regulation of PHGDH in sertoli cells may mediate the process of abnormal glucose metabolism. Our study provide new insight into the mechanisms underlying metabolism-associated male infertility and suggests a novel therapeutic target for male infertility.

12.
World J Surg Oncol ; 18(1): 172, 2020 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-32677962

RESUMO

BACKGROUND: Several genetic driver alterations have been identified in micropapillary lung adenocarcinoma (MPA). However, the frequency of co-alteration of ROS1, EGFR, and EML4-ALK is yet unclear. Herein, we investigated the relationship between clinicopathologic characteristics and well-identified driver mutations of MPA compared with non-micropapillary lung adenocarcinoma (LA). METHODS: Formalin-fixed paraffin-embedded (FFPE) sections derived from lung adenocarcinoma patients who never received adjuvant chemotherapy or radiation therapy prior to surgical resection were collected from October 2016 to June 2019. EGFR mutations, ROS1 rearrangements, and EML4-ALK fusion were identified in a set of 131 MPA and LA cases by using the amplification refractory mutation system (ARMS). The response rate and duration of response were assessed using Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST 1.1). RESULTS: EGFR mutations had occurred in 42 (76.4%) MPA patients and 42 (55.3%) LA patients. Interestingly, ROS1 rearrangements were highly enriched only in the MPA cases (6/55, 10.9%) but rarely in the LA cases (1/76, 1.3%). Furthermore, 7.3% (4/55) MPA samples had double gene mutations, while only 1.3% (1/76) LA cases had double gene alterations. Of 5 patients with harboring two driver oncogene mutations, four patients (80%) obtained partial response, and one patient (20%) suffered recurrence. CONCLUSIONS: A higher prevalence of ROS1 rearrangement or combined mutations of ROS1, EGFR, and EML4-ALK may play a critical role in the tumorigenesis of MPA. These findings provide a novel therapeutic strategy for patients with malignant MPA through combining TKIs than one TKI.

13.
Funct Plant Biol ; 47(11): 977-987, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32645281

RESUMO

To obtain the compatibility of interstocks crossing with 'Yuanxiaochun', we performed a comparative analysis with five interstocks. From the 60th to 240th day after grafting, there was a significant difference between different treatments. All the new shoot/interstock diameter ratios were <1, indicating that there was no obvious phenomenon of small feet in 'Yuanxiaochun' seedlings of five kinds of interstocks. The density of 'Yuanxiaochun' was significantly different. Chl a, Chl b, T-Chl content of 'Shiranuhi', 'Harumi', 'Tarocco' changed greatly from the 90th to 120th day after grafting. The intercellular CO2 concentration (Ci) of 'Shiranuhi' was significantly higher than the other interstocks. In addition, when 'Yuanxiaochun' was grafted onto 'Shiranuhi', net photosynthetic rate (Pn), stomatal conductance (gs) and tanspiration rate (Tr) were higher. When 'Harumi' were used as the interstocks of 'Yuanxiaochun', the light saturation point (LSP) value was larger, which was conducive to the utilisation of strong light. Moreover, the value of LSP-LCP (LCP, light compensation point) of 'Harumi' and 'Tarocco' were significantly higher than the other three interstocks. The apparent quantum efficiency (AQE), RuBP maximum regeneration rate (Jmax) and maximum carboxylation efficiency of Rubisco (Vcmax) value of 'Shiranuhi' was significantly lower than that of 'Ponkan'. The CO2 compensation point (CCP) of 'Harumi' interstock was lower, but the CO2 saturation point (CSP) of 'Tarocco' interstock was higher than those of other interstocks respectively. There was a small difference in initial fluorescence (F0) of different interstocks. The maximal photochemical efficiency of PSII in the dark (Fv/Fm) of 'Kumquat' was the lowest. In addition, Both Y(II) and ETR values of the 'Yuanxiaochun' leaves of 'Ponkan' interstock was the largest one. However, the non-photochemical quenching (qN) of 'Ponkan' was significantly lower, and that of 'Tarocco' interstock was the highest one. Interstocks have different effect on the growth and development, photosynthetic characteristics related to physiological characteristics of 'Yuanxiaochun' trees. 'Ponkan' and 'Kumquat' as the interstock of 'Yuanxiaochun' was more conducive to the rapid accumulation of photosynthetic products for normal vegetative and reproductive growth of plants.

14.
Zhongguo Yi Liao Qi Xie Za Zhi ; 44(3): 189-193, 2020 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-32621423

RESUMO

Magnetic resonance based electrical properties tomography (MREPT) is a different method from proton density imaging, Bloch-Siegert shift (BSS) is used in this paper to reconstruct the radiofrequency (RF) field amplitude and calculate the distribution of the permittivity constant. The phase of the RF field is approximated by the phase component of the magnetization intensity, and the conductivity distribution is calculated. In the experiment, Bruker 7.0 T magnetic resonance device was used to image two water models and in vivo Balb/c mice to obtain the image of electrical characteristics. Experimental results show that the Bloch-siegert B1+ image is significantly more efficient than the double-angle B1+ image. The study can provide a reference for selecting appropriate B1 mapping technology for B1 field imaging of electrical characteristics organizations, and provide basic research support for promoting the practical application of magnetic resonance characteristics.


Assuntos
Imagem por Ressonância Magnética , Tomografia , Algoritmos , Animais , Espectroscopia de Ressonância Magnética , Camundongos , Imagens de Fantasmas
15.
Wei Sheng Yan Jiu ; 49(3): 458-462, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32693897

RESUMO

OBJECTIVE: To investigate the effects of mannose, the major component of Lycium barbarum polysaccharide, and its potential target metabolite, inositol, on mouse islet ß-TC6 cells. METHODS: Different concentrations(0, 4. 6875, 9. 375, 18. 75, 37. 5, 75 and 150 µg/mL) of mannose or inositol were used to intervene ß-TC6 cells for 24 hours, and the proliferation activity of cells was determined by CCK-8 method. Enzyme-linked immunosorbent assay was used to detect insulin secretion after the intervention of the ß-TC6 cells from different concentration of the mannose or inositol(0, 18. 75, 75 and 150 µg/mL) combining with glucose stimulation(20 mmol/L) for 60 minutes. Pioglitazone(3. 92 mg/L) was set up as positive group, and after intervention of the mannose or inositol(0, 9. 375, 18. 75, 75 and 150 µg/mL) for 24 h, the expression levels of insulin, glucose kinase(GK), glucose transporter 4(GLUT4) and glycogen synthase(GS) mRNA were detected by real-time quantitative PCR. RESULTS: Compared with the control group, mannose and inositol promoted the proliferation of ß-TC6 cells in a concentration-dependent manner(18. 75-150 µg/mL)(P<0. 05). Although the inositol solution of 4. 6875 µg/mL and 9. 375 µg/mL had a tendency to promote cell proliferation, there was no statistical difference(P>0. 05). After stimulation with 20 mmol/L glucose combining with different intervention concentrations(18. 75, 75 and 150 µg/mL) of mannose or inositol, no significant difference was observed in the insulin secretion of each group(P>0. 05) comparing with the control group. RT-qPCR result showed that 150 µg/mL mannose increased the expression level of GLUT4(P<0. 01) and the expression levels of GK and GLUT4 genes in the 75 µg/mL inositol group were significantly increased(P<0. 01). The expression level of GLUT4 was improved only when the concentration was decreased to 18. 75 µg/mL in inositol group(P<0. 01). CONCLUSION: Mannose and inositol can improve the expression of GLUT4 mRNA, which may help to increase glucose uptake by peripheral cells. In addition, inositol can improve insulin sensitivity and glucose metabolism by increasing the expression level of GK mRNA.


Assuntos
Lycium , Manose , Animais , Medicamentos de Ervas Chinesas , Glucose , Inositol , Insulina , Camundongos , Polissacarídeos
16.
Huan Jing Ke Xue ; 41(6): 2834-2841, 2020 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608800

RESUMO

Early warning of soil environmental quality is an important basis for implementing classified and graded soil risk management measures. To quickly understand the regional soil environmental quality and take effective measures in time to prevent continuous soil pollution before deterioration of soil environmental quality, a simple, effective, and quantifiable early warning system for soil environmental quality of agricultural land and development land was respectively established based on environmental capacity and pollutant input-output flux theory. Furthermore, corresponding method and mechanism for early warning were defined based on soil environmental quality standards, food safety standards, and carcinogenic risk coefficients. The agricultural land in Youxian county and the development area within the fifth-ring in Beijing were chosen to assess the soil environmental quality and predict risks of heavy metals exceeding standards in different scenarios. The results show that the soil environmental quality of the agricultural land in Wangling and Taoshui Town both can be classified to the fifth early warning level. Compared with other remediation measures, the Cd contents of soil can be lowered to risk screening levels in the short term by the scenario of "paddy straw not returned to the field". The soil quality in the development area within the fifth-ring in Beijing belongs to the first early warning level under both the "no intervention" and the "decreased input" scenarios, which means that Cd, Cu, Pb, and Zn all need more than 50 years to reach their threshold values to pose potential health risks.

17.
Huan Jing Ke Xue ; 41(5): 2373-2380, 2020 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608856

RESUMO

To understand the effect of soil environmental carrying capacity on pollutants and human activities, as well as to effectively prevent the aggravation of soil pollution and control soil environmental risks, a comprehensive indicator system for soil environmental carrying capacity is developed by analyzing the input-output flux and risk characteristics of soil pollutants. Furthermore, an evaluation method for soil environmental carrying capacity is proposed by defining safety coefficients related to evaluation indicators. Based on evaluation of soil environmental quality, the system reflects soil properties, pollution evolution trends, and risk characteristics, focusing on the soil buffering function. Further, a quantitative evaluation is carried out to assess the regional soil environmental carrying capacity of heavy metals on development land in Beijing. The results show that the soil environmental carrying capacity of Cu, Zn, Pb, and Cd in Beijing varies widely. The soil environmental carrying capacity of Cd is much lower than that of other elements. Four policy recommendations are proposed as significant for effective soil pollution prevention and control:clarifying concepts for soil environmental carrying capacity, improving the evaluation framework, constructing an information database, and implementing demonstration pilots.

18.
Aging (Albany NY) ; 12(14): 14066-14079, 2020 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-32699183

RESUMO

OBJECTIVE: To evaluate achievement of comprehensive controls among patients with type 2 diabetes mellitus (T2DM) in different age groups. RESULTS: The elderly patients had higher control rates for BMI (44.36%), TC (50.83%) and LDL-C (48.27%) than those aged 60-80 years and younger patients (all P <0.05). Multiple logistic regression revealed that elderly patients were more likely to achieve control targets for HbA1c (odd ratio (OR) = 2.19), TC (OR = 1.32), HDL-C (OR = 1.35), and TG (OR = 1.74) than younger patients. This effect was stronger in males (ORHbA1c = 2.27; ORTC = 1.41; ORHDL-C = 1.51; ORTG = 1.80). By contrast, elderly females were only more likely to achieve HbA1c < 7.0% (OR=1.88). CONCLUSIONS: Our findings suggest that comprehensive control strategies still should be strengthened. METHODS: A total of 3126 T2DM patients were included, and detected blood pressure (BP), body mass index (BMI), glycosylated hemoglobin (HbA1c), fasting plasma glucose (FPG), postprandial plasma glucose (PPG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C). We divided patients into three age groups (<60, 60-80 and ≥ 80 years), to assess the differences in achieving the control targets.

19.
Environ Sci Pollut Res Int ; 27(31): 39094-39104, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32638307

RESUMO

Two pot experiments were conducted to study the effects of intercropping cadmium (Cd) accumulator plants (Stellaria media (L.) Villars, Cardamine hirsuta, Cerastium glomeratum Thuill, and Galium aparine L.) and applying their straw on the growth and Cd accumulation of Brassica chinensis L. Intercropping with four accumulator plants reduced the biomass, water content, and photosynthetic pigment content of B. chinensis compared with monoculture. Intercropping with accumulator plants increased the Cd content in the roots and shoot of B. chinensis, and the translocation factor (TF), root bioconcentration factor (root BCF), and shoot bioconcentration factor (Shoot BCF) increased. The soil pH decreased and the soil available Cd content increased by intercropping. Thus, intercropping with four accumulator plants can promote the Cd uptake of B. chinensis. The straw of four accumulator plants reduced the biomass, water content, and photosynthetic pigment content of B. chinensis compared with the control. The straw of S. media and C. hirsute increased the Cd content in the roots and shoots of B. chinensis, TF, root BCF, and shoot BCF. The straw of C. glomeratum and G. aparine decreased the Cd content in the roots and shoots of B. chinensis, TF, root BCF, and shoot BCF. The soil pH increased and the soil available Cd content decreased by application of straw. Thus, the straw of C. glomeratum and G. aparine can reduce the Cd uptake of B. chinensis.


Assuntos
Brassica , Poluentes do Solo/análise , Biodegradação Ambiental , Cádmio/análise , Raízes de Plantas/química , Solo
20.
PLoS Negl Trop Dis ; 14(6): e0008255, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32530913

RESUMO

BACKGROUND: Vivax malaria is an important public health problem in the Greater Mekong Subregion (GMS), including the China-Myanmar border. Previous studies have found that Plasmodium vivax has decreased sensitivity to antimalarial drugs in some areas of the GMS, but the sensitivity of P. vivax to antimalarial drugs is unclear in the China-Myanmar border. Here, we investigate the drug sensitivity profile and genetic variations for two drug resistance related genes in P. vivax isolates to provide baseline information for future drug studies in the China-Myanmar border. METHODOLOGY/PRINCIPAL FINDINGS: A total of 64 P. vivax clinical isolates collected from the China-Myanmar border area were assessed for ex vivo susceptibility to eight antimalarial drugs by the schizont maturation assay. The medians of IC50 (half-maximum inhibitory concentrations) for chloroquine, mefloquine, pyronaridine, piperaquine, quinine, artesunate, artemether, dihydroartemisinin were 84.2 nM, 34.9 nM, 4.0 nM, 22.3 nM, 41.4 nM, 2.8 nM, 2.1 nM and 2.0 nM, respectively. Twelve P. vivax clinical isolates were found over the cut-off IC50 value (220 nM) for chloroquine resistance. In addition, sequence polymorphisms in pvmdr1 (P. vivax multidrug resistance-1), pvcrt-o (P. vivax chloroquine resistance transporter-o), and difference in pvmdr1 copy number were studied. Sequencing of the pvmdr1 gene in 52 samples identified 12 amino acid substitutions, among which two (G698S and T958M) were fixed, M908L were present in 98.1% of the isolates, while Y976F and F1076L were present in 3.8% and 78.8% of the isolates, respectively. Amplification of the pvmdr1 gene was only detected in 4.8% of the samples. Sequencing of the pvcrt-o in 59 parasite isolates identified a single lysine insertion at position 10 in 32.2% of the isolates. The pvmdr1 M908L substitutions in pvmdr1 in our samples was associated with reduced sensitivity to chloroquine, mefloquine, pyronaridine, piperaquine, quinine, artesunate and dihydroartemisinin. CONCLUSIONS: Our findings depict a drug sensitivity profile and genetic variations of the P. vivax isolates from the China-Myanmar border area, and suggest possible emergence of chloroquine resistant P. vivax isolates in the region, which demands further efforts for resistance monitoring and mechanism studies.


Assuntos
Antimaláricos/farmacologia , Resistência a Medicamentos , Malária Vivax/parasitologia , Proteínas de Membrana Transportadoras/genética , Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética , Plasmodium vivax/efeitos dos fármacos , Polimorfismo Genético , Proteínas de Protozoários/genética , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China , Feminino , Genótipo , Humanos , Lactente , Concentração Inibidora 50 , Masculino , Pessoa de Meia-Idade , Mianmar , Testes de Sensibilidade Parasitária , Plasmodium vivax/isolamento & purificação , Análise de Sequência de DNA , Adulto Jovem
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