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1.
Int J Mol Sci ; 21(3)2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32013029

RESUMO

Although melatonin was affirmed to alleviate drought stress in various plant species, the mechanism in kiwifruit remains to be elucidated. In this study, the transcriptomes of kiwifruit leaves under control (CK), DR (drought stress), and MTDR (drought plus melatonin) treatments were evaluated. After comparisons of the gene expression between DR and MTDR, the differentially expressed genes (DEGs) were screened. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated three significant pathways, which were mainly involved in the glutathione metabolism, ascorbate and aldarate metabolism, and carotenoid metabolism. Therefore, the content and metabolic gene expression level of ascorbic acid (AsA), glutathione, and carotenoid were higher in the MTDR treatment than that in others. Furthermore, the activity and mRNA expression level of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were also promoted in the MTDR group. Combined with these results of important secondary metabolites and protective enzymes measured in the seedlings in different treatments, it could be concluded that exogenous melatonin induced the ascorbic acid-glutathione (AsA-GSH) cycle, carotenoid biosynthesis, and protective enzyme system to improve seedling growth. Our results contribute to the development of a practical method for kiwifruit against drought stress.

2.
Aging (Albany NY) ; 12(2): 1843-1856, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32005028

RESUMO

BACKGROUNDS: Emerging evidences has demonstrated that dysregulation of long non-coding RNAs (lncRNAs) is critically involved in esophageal squamous cell carcinoma (ESCC) progression. However, the function of lncRNA PSMA3-AS1 in ESCC is unclear. Therefore, we aimed to explore the functions and potential mechanisms of PSMA3-AS1 in ESCC cells progression. RESULTS: Here, we found that PSMA3-AS1 expression was significantly up-regulated in ESCC tissues. Forced PSMA3-AS1 expression was correlated with tumor size, distant metastasis, and poor prognosis in ESCC patients. Functionally, PSMA3-AS1-overexpression promoted ESCC cells proliferation, invasion, and migration in vitro. Mechanistically, PSMA3-AS1 up-regulated EZH2 expression by competitively binding to miR-101. CONCLUSION: PSMA3-AS1 is significantly up-regulated in ESCC tissues, and the PSMA3-AS1/miR-101/EZH2 axis plays a critical role in ESCC progression. Taken together, our results may provide promising targets for ESCC therapy. METHODS: PSMA3-AS1 and miR-101 expression were explored using qRT-PCR in ESCC tissues and cell lines. Immunohistochemistry assays were carried out to analyze EZH2 (enhancer of zeste homolog) protein expression. RIP, dual-luciferase reporter, fluorescence in situ hybridization, and biotin pull-down assays were used to detect the interactions of PSMA3-AS1, miR-101 and EZH2. The biological functions of PSMA3-AS1 in PSMA3-AS1-altered cells were explored using CCK-8, colony formation, wound healing, and transwell assays in vitro.

3.
Molecules ; 25(3)2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32050515

RESUMO

Although the effects of melatonin on plant abiotic and biotic stress resistance have been explored in recent decades, the accumulation of endogenous melatonin in plants and its influence on fruit quality remains unclear. In the present study, melatonin accumulation levels and the expression profiles of five synthesis genes were investigated during fruit and leaf development in sweet cherry (Prunus avium L.). Melatonin was strongly accumulated in young fruits and leaves, then decreased steadily with maturation. Transcript levels of PacTDC and PacSNAT were highly correlated with melatonin content in both fruit and leaves, indicating their importance in melatonin accumulation. Furthermore, application of 50 and 100 µmol·L-1 of melatonin to leaves had a greater influence on fruit quality than treatments applied to fruits, by significantly improving fruit weight, soluble solids content, and phenolic content including total phenols, flavanols, total anthocyanins, and ascorbic acid. Meanwhile, melatonin application promoted the antioxidant capacity of fruit assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylben zothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP). These results provide insights into the physiological and molecular mechanisms underlying melatonin metabolism of sweet cherry.

4.
Oncol Rep ; 43(3): 1010-1018, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32020220

RESUMO

The efficacy of chemotherapy for hepatocellular carcinoma (HCC) remains unsatisfactory, primarily due to inherent self­defense mechanisms (e.g., mitophagy and autophagy). In the present study, we aimed to explore the pro­apoptotic effects of targeting mitophagy to potentiate the efficacy of chemotherapy for HCC. HCC cells were subjected to cisplatin, after which cisplatin­induced mitophagy was quantified by immunofluorescence. Mdivi­1, a specific dynamin­related protein 1 (DRP1) inhibitor, was used to study the role of DRP1 in cisplatin­induced HCC mitophagy. The synergistic effect of cisplatin and the DRP1 inhibitor on HCC was assessed in vitro and in vivo. Accordingly, cisplatin induced mitophagy in surviving HCC cells by activating DRP1. The DRP1 inhibitor (Mdivi­1) increased the apoptosis of cisplatin­treated HCC cells by targeting mitophagy. Mechanistically, Mdivi­1 upregulated Bax and downregulated Bcl­xL, leading to an increase in mitochondrial membrane permeability and subsequent release of cytochrome c from mitochondria into the cytosol, thereby aggravating cisplatin­induced apoptosis in HCC cells. Moreover, Mdivi­1 acted synergistically with cisplatin to suppress HCC xenograft growth in vivo. Our results indicate that targeting cisplatin­mediated mitophagy increases HCC apoptosis via DRP1 inhibition, providing preclinical proof of concept for combination therapy targeting mitophagy to potentiate the efficacy of chemotherapy.

5.
Exp Neurol ; 327: 113223, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32032565

RESUMO

AIMS: Ischemic stroke has become one of the main causes of death worldwide. MicroRNAs (miRNAs) have been implicated in cerebral ischemia-reperfusion (I/R) injury and could serve as therapeutic targets. 5-Lipoxygenase (5-LOX) is a key enzyme in the biosynthesis of leukotrienes and has been implicated in inflammatory central nerve system disorders. The objective of this study was to explore the neuroprotective effects of miR-193b-3p against focal cerebral I/R injury in rats by regulating 5-LOX expression. METHODS AND MATERIALS: Adult male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion and reperfusion injury. The level of miR-193b-3p expression was observed in the rat cortical peri-infarct region after focal cerebral I/R injury. Bioinformatics analysis was used to predict the binding sites of miR-193b-3p, and a dual-luciferase reporter gene assay was applied to verify the potential interaction between 5-LOX mRNA and miR-193b-3p. Then, rats were injected with a miR-193b-3p agomir (modified and enhanced mimic) or antagomir (modified and enhanced inhibitor) in the right lateral ventricle of the brain. Neurological deficit scores, infarct volumes, neuron damage and 5-LOX enzymatic activity and expression were measured. In an in vitro experiment, cultured PC12 cells were exposed to oxygen-glucose deprivation and reperfusion (OGD/R). OGD/R-induced cells were treated with a miR-193b-3p mimic or inhibitor and 5-LOX siRNA. Cell viability, lactate dehydrogenase release, apoptosis rate and 5-LOX expression were evaluated. RESULTS: The level of miR-193b-3p expression was increased in the cortical peri-infarct region of rats with cerebral focal I/R injury. The results of the dual-luciferase reporter gene assay showed that a miR-193b-3p binding site was located in the 3' untranslated region (3'UTR) of 5-LOX mRNA. Neurological deficit scores, infarct volumes and neuronal injury were alleviated by miR-193b-3p agomir treatment but aggravated by miR-193b-3p antagomir. Furthermore, leukotriene B4, cysteinyl-leukotrienes and 5-LOX expression in the cortical peri-infarct region of rats with focal cerebral I/R injury were also downregulated by miR-193b-3p agomir treatment but upregulated by miR-193b-3p antagomir. In PC12 cells, miR-193b-3p mimic significantly decreased OGD/R-induced cell death and reduced lactate dehydrogenase release and 5-LOX expression. In contrast, miR-193b-3p inhibitor exacerbated OGD/R-induced injury in PC12 cells. Additionally, the in vitro effects of miR-193b-3p inhibitor on OGD/R-induced cell injury were partially reversed by 5-LOX siRNA treatment. CONCLUSION: MiR-193b-3p has a potentially neuroprotective effect on focal cerebral I/R-induced injury by inhibiting 5-LOX expression.

6.
Front Med ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31938981

RESUMO

The aim of this study was to characterize rpoC gene mutations in Mycobacterium tuberculosis (MTB) and investigate the factors associated with rpoC mutations and the relation between rpoC mutations and tuberculosis (TB) transmission. A total of 245 MTB clinical isolates from patients with TB in six provinces and two municipalities in China were characterized based on gene mutations through DNA sequencing of rpoC and rpoB genes, phenotyping via standard drug susceptibility testing, and genotypic profiling by mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing. Approximately 36.4% of the rifampin-resistant isolates harbored nonsynonymous mutations in the rpoC gene. Twenty-nine nonsynonymous single mutations and three double mutations were identified. The rpoC mutations at locus 483 (11.3%) were predominant, and the mutations at V483G, W484G, I491V, L516P, L566R, N698K, and A788E accounted for 54.5% of the total detected mutations. Fifteen new mutations in the rpoC gene were identified. Rifampin resistance and rpoB mutations at locus 531 were significantly associated with rpoC mutations. MIRU-VNTR genotype results indicated that 18.4% of the studied isolates were clustered, and the rpoC mutations were not significantly associated with MIRU-VNTR clusters. A large proportion of rpoC mutation was observed in the rifampicin-resistant MTB isolates. However, the findings of this study do not support the association of rpoC mutation with compensated transmissibility.

7.
J Environ Manage ; 256: 109943, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31989978

RESUMO

Magnetic graphene oxide-titanate composites (MGO@TNs) were synthesized via growing titanate nanosheets on the graphene oxide sheets with magnetite nanoparticles anchored on. The as-prepared MGO@TNs showed a hierarchical structure and large specific surface area (193.4 m2/g), which were suitable for rapid and effective adsorption of Pb(II) from wastewater. Moreover, the loaded magnetite nanoparticles guaranteed the effective magnetic separation of MGO@TNs, avoiding secondary pollution. The adsorption mechanism were illuminated to be ion exchange and surface complexation. Batch adsorption experiments showed the maximum adsorption capacity of MGO@TNs reached 322.7 mg/g for Pb(II) removal. The removal efficiency retained 89.6% after six adsorption-desorption cycles. In addition, the efficiency reached up to 99.8% when applying MGO@TNs for removal of Pb(II) from simulated realistic battery wastewater, ensuring the safe discharge of treated water. The good adsorption performance, recyclability and easy magnetic separation ability made sure that the MGO@TNs has great potential for purification of Pb(II) contaminated wastewater.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Adsorção , Grafite , Cinética , Chumbo , Fenômenos Magnéticos , Águas Residuárias
8.
J Photochem Photobiol B ; 203: 111779, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31927487

RESUMO

Light is a key environmental factors affecting anthocyanin accumulation in plants. Ubiquitin E3 ligase COP1 has been proved to be a negative regulator involved in light-regulated plant development process, whereas the function and expression specificity of COP1 in anthocyanin biosynthesis in sweet cherry remains unclear. In the present study, we identified a COP1 in sweet cherry, named PacCOP1, it exhibited apparent different expression patterns in red-colored 'Hongdeng' and bi-colored 'Satonishiki', with increasing trend largely in 'Satonishiki', but decreasing trend in 'Hongdeng' after veraison, which was contrary to their variation tendency of anthocyanin content. While the expression abundance of anthocyanin biosynthesis related genes were largely increased after veraison, in accordance with anthocyanin content. Correlation analysis proved that the expression of PacCOP1 was negative correlated with the major genes on anthocyanin accumulation in 'Hongdeng' and 'Satonishiki' fruit, in especial PacDFR, PacANS, PacMYBA and PacbHLH33. Furthermore, over-expression of PacCOP1 in Arabidopsis displayed increased COP1 transcript level with negligible pigmentation and corresponding lower expression level of AtPAP1, AtDFR, AtLDOX, and AtUFGT. These results revealed the negative regular role of PacCOP1 in anthocyanin biosynthesis by repressing the PacMYBA transcription level, followed by down-regulating the structural genes expression abundance, eventually leading to attenuated anthocyanin accumulation in fruits.


Assuntos
Antocianinas/biossíntese , Proteínas de Plantas/metabolismo , Prunus avium/enzimologia , Ubiquitina-Proteína Ligases/metabolismo , Sequência de Aminoácidos , Arabidopsis/metabolismo , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Filogenia , Pigmentação/genética , Proteínas de Plantas/classificação , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/metabolismo , Prunus avium/metabolismo , Alinhamento de Sequência , Ubiquitina-Proteína Ligases/classificação , Ubiquitina-Proteína Ligases/genética
9.
Nanotechnology ; 31(18): 185402, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31952062

RESUMO

SnO2/α-Fe2O3 hybrid nanofibers, consisting of one dimensional hollow SnO2 nanotubes decorated with Fe2O3 particles, are synthesized via a facile electrospinning process followed by a hydrothermal treatment and investigated as the anode material for lithium-ion batteries. The SnO2/Fe2O3 hybrid nanofibers not only provide a large surface area for lithium storage sites, but also show the capability of fast charge transport for both electrons and lithium ions. Both SnO2 and Fe2O3 contribute to the lithium storage and the hybrid structure exhibits a synergistic effect when participating in electrochemical reactions. With the smart structure design, the hybrid SnO2/Fe2O3 nanofiber electrode shows superior electrochemical performance compared to the bare SnO2 nanofiber electrode. In addition to a large first-cycle discharge capacity up to 1105 mAh g-1, the hybrid SnO2/Fe2O3 nanofibers electrode also exhibits good cycling stability and high rate capability.

10.
Medicine (Baltimore) ; 99(2): e17908, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914010

RESUMO

This study is to determine accuracy of abdominal ultrasound and nuclear magnetic resonance imaging (MRI) for placenta accreta in the second and third trimester of pregnancy and to define the most relevant features of abdominal ultrasound and MRI for placenta accreta prediction.Between September 2012 and September 2018, 245 high risk of placenta accreta in the second trimester of pregnancy were prenatal diagnosed by abdominal ultrasound and MRI and they were followed up until the end of pregnancy.Forty-six patients at the second trimester of pregnancy and 40 patients at the third trimester of pregnancy were confirmed as placenta accreta. For the second and third trimester of pregnancy, the sensitivity (Se), specificity (Sp), positive and negative predictive value (PPV and NPV) of abdominal ultrasound were 95.65% versus 97.50%, 91.78% versus 90.70%, 88% versus 83%, and 97% versus 99%, respectively, while the Se, Sp, PPV, and NPV of MRI were 89.13% versus 92.50%, 87.67% versus 8721%, 82% versus 77%, and 93% versus 96%, respectively. Five features having significant statistical differences between normal placentation women and placenta accreta patients in second or third trimester of pregnancy, including loss of the normal retroplacental clear space, thinning or disappearance of the myometrium, increased vascularization at the uterine serosa-bladder wall interface, and vascularization perpendicular to the uterine wall on abdominal ultrasound, and uterine bulging and dark intraplacental bands on MRI.Abdominal ultrasound and MRI for placenta accreta in the second and third trimester of pregnancy could provide meaningful imaging evidences.


Assuntos
Imagem por Ressonância Magnética/métodos , Placenta Acreta/diagnóstico por imagem , Placenta/anormalidades , Ultrassonografia/métodos , Útero/diagnóstico por imagem , Adulto , Feminino , Humanos , Miométrio/patologia , Placenta Acreta/patologia , Valor Preditivo dos Testes , Gravidez , Segundo Trimestre da Gravidez , Terceiro Trimestre da Gravidez , Sensibilidade e Especificidade , Ultrassonografia Pré-Natal/normas , Ultrassonografia Pré-Natal/estatística & dados numéricos , Útero/irrigação sanguínea , Útero/patologia
11.
ACS Appl Mater Interfaces ; 12(2): 2671-2678, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31899615

RESUMO

Transition-metal sulfides have been considered as promising anode materials for lithium-ion batteries (LIBs) due to their high theoretical specific capacity and superior electrochemical performance. However, the large volume change during the discharge/charge process causes structural pulverization, resulting in rapid capacity decline and the loss of active materials. Herein, we report Co1-xS hollow spheres formed by in situ growth on reduced graphene oxide layers. When evaluated as an anode material for LIBs, it delivers a specific capacity of 969.8 mAh·g-1 with a high Coulombic efficiency of 96.49% after 90 cycles. Furthermore, a high reversible capacity of 527.2 mAh·g-1 after the 107th cycle at a current density of 2.5 A g-1 is still achieved. The results illustrate that in situ growth on the graphene layers can enhance conductivity and restrain volume expansion of cobalt sulfide compared with ex situ growth.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31942722

RESUMO

Antibiotic-heavy metal multi-pollutants are produced by intensive livestock farming and become an increasingly prominent problem. In this study, the transport behavior of tetracycline (TC) and its chelate with copper ions (Cu-TC) in saturated sand column with and without graphene oxide (GO) prefilled was investigated by laboratory breakthrough experiments. The effects of pH, ionic strength, and the cotransport with GO were studied detailedly. The results showed that the prepared nano-GO had a single- or multilayered sheet structure with a diameter of several µm. The surface of GO contained abundant oxygen-containing functional groups, which imparted it strong hydrophilicity and electronegativity. Pollutant transport experiments showed that decrease of H+ weakened the transport ability of TC and Cu-TC. Both Na+ and Ca2+ promoted the transport of TC, with Ca2+ having a much greater effect. The presence of Na+ inhibited the transport of Cu-TC, while Ca2+ promoted Cu-TC transport. The addition of Cu2+ was more favorable for the transport of Cu-TC than TC alone. In the GO-prefilled column, the effluent concentrations of TC and Cu-TC greatly decreased due to adsorption onto GO surfaces. The transport of Cu-TC was more related to GO concentration than TC alone due to the high affinity between GO and Cu-TC. Moreover, the transport behavior of GO in the sand column was consistent with that of the corresponding TC or Cu-TC, indicating that GO could cotransport with TC and Cu-TC multi-pollutants. Our study showed that the GO would interact with TC and Cu-TC and thus have significant influences on the fate and transport of these pollutions in porous media.

13.
BMC Infect Dis ; 20(1): 19, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910878

RESUMO

BACKGROUND: Pyrazinamide still may be a useful drug for treatment of rifampin-resistant (RR-TB) or multidrug-resistant tuberculosis (MDR-TB) in China while awaiting scale up of new drugs and regimens including bedaquiline and linezolid. The level of pyrazinamide resistance among MDR-TB patients in China is not well established. Therefore, we assessed pyrazinamide resistance in a representative sample and explored determinants and patterns of pncA mutations. METHODS: MDR-TB isolates from the 2007 national drug resistance survey of China were sub-cultured and examined for pyrazinamide susceptibility by BACTEC MGIT 960 method. pncA mutations were identified by sequencing. Characteristics associated with pyrazinamide resistance were analyzed using univariable and multivariable log-binominal regression. RESULTS: Of 401 MDR-TB isolates, 324 were successfully sub-cultured and underwent drug susceptibility testing. Pyrazinamide resistance was prevalent in 40.7% of samples, similarly among new and previously treated MDR-TB patients. Pyrazinamide resistance in MDR-TB patients was associated with lower age (adjusted OR 0.54; 95% CI, 0.34-0.87 for those aged ≧60 years compared to < 40 years). Pyrazinamide resistance was not associated with gender, residential area, previous treatment history and Beijing genotype. Of 132 patients with pyrazinamide resistant MDR-TB, 97 (73.5%) had a mutation in the pncA gene; with 61 different point mutations causing amino acid change, and 11 frameshifts in the pncA gene. The mutations were scattered throughout the whole pncA gene and no hot spot region was identified. CONCLUSIONS: Pyrazinamide resistance among MDR-TB patients in China is common, although less so in elderly patients. Therefore, pyrazinamide should only be used for treatment of RR/MDR-TB in China if susceptibility is confirmed. Molecular testing for detection of pyrazinamide resistance only based on pncA mutations has certain value for the rapid detection of pyrazinamide resistance in MDR-TB strains but other gene mutations conferring to pyrazinamide resistance still need to be explored to increase its predictive ability .

14.
J Microbiol Biotechnol ; 30(1): 127-135, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31693839

RESUMO

Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and ß-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.

15.
Bioresour Technol ; 297: 122451, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31787516

RESUMO

This study used a metagenomic approach to investigate the effects of earthworms on ARGs and HPB during the vermicomposting of dewatered sludge. Results showed that 139 types of ARGs were found in sludge vermicompost, affiliated to 30 classes. Compared with the control, the total abundance of ARGs in sludge vermicompost decreased by 41.5%. Moreover, the types and sequences of plasmids and integrons were also decreased by vermicomposting. Proteobacteria and Actinobacteria were the most dominant hosts of ARGs in sludge vermicompost. In addition, earthworms reduced the total HPB abundance and modified their diversity, thus leading to higher abundance of Enterobacteriaceae in sludge vermicompost. However, the sludge vermicompost was still ARG and HPB enriched, indicating a remaining environmental risk for agricultural purpose. The observed change of microbial community and the reduction of mobile genetic elements caused by earthworm activity are the main reasons for the alleviation of ARG pollution during vermicomposting.


Assuntos
Oligoquetos , Animais , Antibacterianos , Bactérias , Resistência Microbiana a Medicamentos , Genes Bacterianos , Humanos , Esgotos
16.
J Gastroenterol ; 55(2): 142-158, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31845054

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of dysregulated lipid and glucose metabolism, which is often associated with obesity, dyslipidemia and insulin resistance. In view of the high morbidity and health risks of NAFLD, the lack of effective cure has drawn great attention. In recent years, a line of evidence has suggested a close linkage between the intestine and liver diseases such as NAFLD. We summarized the composition and characteristics of intestinal microbes and reviewed molecular insights into the intestinal microbiome in development and progression of NAFLD. Intestinal microbes mainly include bacteria, archaea, viruses and fungi, and the crosstalk between non-bacterial intestinal microbes and human liver diseases should be paid more attention. Intestinal microbiota imbalance may not only increase the intestinal permeability to gut microbes but also lead to liver exposure to harmful substances that promote hepatic lipogenesis and fibrosis. Furthermore, we focused on reviewing the latest "gut-liver axis"-targeting treatment, including the application of antibiotics, probiotics, prebiotics, synbiotics, farnesoid X receptor agonists, bile acid sequestrants, gut-derived hormones, adsorbents and fecal microbiota transplantation for NAFLD. In this review, we also discussed the potential mechanisms of "gut-liver axis" manipulation and efficacy of these therapeutic strategies for NAFLD treatment.

17.
Int J Phytoremediation ; 22(1): 62-68, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31321987

RESUMO

A pot experiment was conducted to study the effects of different rootstocks on the cadmium (Cd) accumulation characteristics of the post-grafting generations of Cd-hyperaccumulator Galinsoga parviflora plants. Five treatments, ungrafted and G. parviflora seedlings grafted on the rootstocks of Kalimeris indica, Senecio scandens, Conyza canadensis, and Artemisia sieversiana, were utilized. The four rootstock grafts decreased the shoot biomass of the G. parviflora post-grafting generation compared with ungrafted. The K. indica and S. scandens grafts increased the Cd concentration in shoots of the G. parviflora post-grafting generation by 15.06% and 14.40%, respectively, compared with ungrafted, while the C. canadensis and A. sieversiana grafts had no significant effects. K. indica grafts increased the amount of Cd extracted by shoots of the G. parviflora post-grafting generation by 10.59% compared with ungrafted, while the other treatments resulted in decreases. Compared with ungrafted, the different rootstocks had no significant effects on the photosynthetic pigment content of the G. parviflora post-grafting generation, and only C. canadensis grafts increased the superoxide dismutase activity level, while only K. indica grafts increased the peroxidase activity level. Therefore, the K. indica rootstock could increase the phytoremediation capability of G. parviflora post-grafted plants grown in Cd-contaminated soil.


Assuntos
Cádmio , Poluentes do Solo , Biodegradação Ambiental , Raízes de Plantas , Plântula
18.
Zhongguo Zhen Jiu ; 39(12): 1274-8, 2019 Dec 12.
Artigo em Chinês | MEDLINE | ID: mdl-31820601

RESUMO

OBJECTIVE: To explore the clinical efficacy of acupuncture combined with Jingtong granule for nerve-root type cervical spondylosis and its effects on serum interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1ß) and hemorheological indexes. METHODS: A total of 114 patients with nerve-root type cervical spondylosis were randomly divided into an observation group and a control group, 57 cases in each group. The patients in both groups were treated with traction. The patients in the control group were treated with oral administration of Jingtong granule, 4 g each time, 3 times a day, while based on the treatment of control group, the patients in the observation group were treated with acupuncture at Dazhui (GV 14), Tianzhu (BL 10), Houxi (SI 3), cervical Jiaji (EX-B 2), Quchi (LI 11), Hegu (LI 4) and Waiguan (TE 5), once a day. Both groups were treated for 4 weeks. The simplified McGill pain questionnaire (MPQ), neck disability index (NDI), numbness score, levels of IL-6, TNF-α, IL-1ß in serum and hemorheological indexes were observed before and after treatment, and the clinical efficacy was compared between the two groups. RESULTS: The total effective rate was 91.2% (52/57) in the observation group, which was higher than 71.9% (41/57) in the control group (P<0.05). Compared before treatment, the scores of MPQ, NDI and numbness in the two groups were reduced after treatment (P<0.05). After treatment, the scores of MPQ, NDI and numbness in the observation group were lower than those in the control group (P<0.05). After treatment, the serum levels of IL-6, TNF-α and IL-1ß in the two groups were reduced (P<0.05), and those in the observation group were lower than the control group (P<0.05). After treatment, the plasma viscosity, fibrinogen, low shear rate of whole blood viscosity and high shear rate of whole blood viscosity in the two groups were lower than before treatment (P<0.05), and those in the observation group were lower than the control group (P<0.05). CONCLUSION: Acupuncture combined with Jingtong granule have significant clinical efficacy for nerve-root type cervical spondylosis, which could reduce the serum levels of IL-6, TNF-α and IL-1ß and improve hemorheology.


Assuntos
Terapia por Acupuntura , Espondilose , Humanos , Interleucina-1beta , Interleucina-6 , Espondilose/terapia , Fator de Necrose Tumoral alfa
19.
Huan Jing Ke Xue ; 40(6): 2954-2960, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854691

RESUMO

Dewatered sludge often contains abundant antibiotics, and such contaminants can potentially affect the nitrogen fertilizer value of sludge-derived vermicompost. For this purpose, the aims of this study were to investigate the effects of different tetracycline concentrations in sludge on the nitrification rate and abundance and community characteristics of ammonia oxidizers during vermicomposting. Four sludge treatments with different concentrations of tetracycline (0, 100, 500, and 1000 mg·kg-1) were used, and data were collected to reveal the mechanism regarding the ammonia oxidization of sludge in the vermicomposting system. The results showed that the high concentration of tetracycline (1000 mg·kg-1) significantly inhibited the nitrification rate of vermicomposting (P<0.05), but no significant effect was observed in the low concentration treatments. Compared with ammonia-oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) were detected as the dominant microorganisms involved in the ammonia oxidation process during vermicomposting. In addition, the abundances of amoA genes were significantly elevated (P<0.05) in the treatments with 100 mg·kg-1 tetracycline, but values significantly decreased with the further increments of tetracycline concentrations (P<0.05). The results obtained from high-throughput sequencing revealed that the community diversity and structure of AOA and AOB were strongly modified by tetracycline concentrations, and a negative correlation relationship was detected between the tetracycline concentrations and the α diversity of AOA.


Assuntos
Amônia/metabolismo , Antibacterianos/análise , Compostagem/métodos , Esgotos/microbiologia , Tetraciclina/análise , Animais , Archaea/classificação , Bactérias/classificação , Nitrificação , Oligoquetos , Oxirredução , Filogenia , Microbiologia do Solo
20.
Huan Jing Ke Xue ; 40(7): 3263-3269, 2019 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854727

RESUMO

The high content of tetracycline in municipal sludge may result in vermicomposting products carrying a high abundance of antibiotic resistance genes, thus lowering the utilization value of the vermicompost. Hence, this study aimed to reveal the underlying effects of tetracycline concentrations on the resistance genes involved in vermicomposting systems for sludge recycling. For this purpose, fresh sludge substrates with different concentrations of tetracycline (100, 500, and 1000 mg·kg-1) were vermicomposted for 60 days using Eisenia foetida. In parallel, sludge treatment without the addition of the tetracycline was used as a control. During the experiment, changes in bacterial communities, tetracycline resistance genes (tetC, tetG, tetM, tetW, and tetX), and class 1 integron (intI1) were detected using high-throughput sequencing and qualitative PCR, respectively. The results showed that the addition of tetracycline reduced the abundance of Proteobacteria but increased the abundance of Bacteroidetes in the sludge vermicompost. Furthermore, the concentrations of tetracycline had a significant negative correlation with Shannon and Pielou indexes of bacterial diversity. In addition, tetracycline increased intI1 genes and tetracycline resistance genes in the sludge by 4.25 times and 4.7-186.9 times, respectively. Moreover, there was a significantly positive correlation between the abundance of tetM genes and tetracycline concentration. This study suggests that higher concentration of tetracycline in sludge can modify the microbial community structure of vermicompost, enhancing the abundance of antibiotic resistance genes and their associated dissemination risks.


Assuntos
Antibacterianos/farmacologia , Compostagem , Resistência Microbiana a Medicamentos/genética , Microbiota , Esgotos/microbiologia , Tetraciclina/farmacologia , Animais , Genes Bacterianos , Oligoquetos , Resistência a Tetraciclina/genética
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