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1.
J Hazard Mater ; 398: 122971, 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32512455

RESUMO

Insect cytochrome P450 s (P450 s) are associated with the metabolic detoxification of toxic xenobiotics and their constitutive upregulation is often associated with resistance to natural and synthetic toxicants. The P450 s CYP321A16 and CYP332A1 are constitutively overexpressed in an insecticide-resistant strain of beet armyworm, Spodoptera exigua. However, the function and upstream regulation of these two P450 s remain unknown. Here, we investigated the function of CYP321A16 and CYP332A1 in resistance to the organophosphate insecticide, chlorpyrifos and their regulation by the transcription factors CncC and Maf. Transgenic strains of Drosophila melanogaster expressing CYP321A16 or CYP332A1 showed higher levels of tolerance to chlorpyrifos than the control flies with the same genetic background. Furthermore, recombinant CYP321A16 and CYP332A1 proteins metabolized chlorpyrifos. Analysis of the putative promoter sequences of the genes coding for CYP321A16 and CYP332A1 revealed conserved CncC/Maf binding sites. Transfection of luciferase reporter plasmids containing the promoter of CYP450 gene together with CncC and Maf expression plasmids significantly enhanced the activity of the reporter. Promoter truncation identified a site in the promoter of CYP321A16 that is critical for the CncC/Maf binding. These data demonstrate that resistance to chlorpyrifos in S. exigua is conferred by the combined action of CYP321A16 and CYP332A1 and uncovered their regulation by the transcription factors CncC and Maf.

2.
J Diabetes ; 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32506614

RESUMO

BACKGROUND: Studies suggested that eosinophils in diabetes might be associated with severity of diabetic nephropathy (DN). In a retrospective study of 102 Chinese patients with biopsy proven DN, we aimed to evaluate relationships of both blood and renal eosinophils (Eos) to the severity of DN, and check whether it can be served as an indicator of prognosis as well as therapeutic effect of steroids. METHODS: 102 patients diagnosed with DN were enrolled. Demographical and clinical data and histopathological scores were associated. Interstitial eosinophilic aggregates (IEA) were defined as the presence of ≥10 Eos in at least one high-power field. End stage renal disease was defined as the end point. RESULTS: We observed that log2 (blood eosinophil counts) correlated with neutrophil counts, proteinuria and tubulointerstitial inflammatory cell infiltration. IEA was observed in 33.3% of the DN patients and was associated with decreased eGFR, higher proteinuria, hematuria, higher HbA1c, increased blood eosinophil counts, tubular injury, tubulointerstitial chronicity and interstitial inflammation. IEA was associated with worse renal prognosis (HR 2.424, P = 0.008). Consistently, urine eosinophil cationic protein (ECP) (ng/mgCr) was associated with renal injury and poor renal prognosis (HR 1.173, P = 0.020). Patients with IEA were more likely to be treated with steroid/immunosuppressants (47.1% vs 14.7%, P = 0.001) but did not show renal benefit. CONCLUSIONS: It suggested that both blood and renal infiltrated eosinophils were prevalent in DN and associated with severity of DN. IEA in renal pathology showed better fit in correlation with renal prognosis. Treatment with steroid/immunosuppressants showed no significant improvement regarding renal prognosis. This article is protected by copyright. All rights reserved.

4.
J Am Chem Soc ; 142(16): 7322-7327, 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32267146

RESUMO

The first copper-catalyzed atroposelective Michael-type addition between azonaphthalenes and arylboronic acids for the construction of biaryl atropisomers was established using a novel BINOL-derived phosphoramidite as a chiral ligand. A broad range of atropisomeric biaryls were obtained with good efficiency, and the practicality of this approach was verified by versatile transformations toward axially chiral ligands, catalysts, and other functional atropisomers. This set of catalytic systems successfully inhibited the routine 1,2-addition and promoted the formation of an aryl-aryl chiral axis. Meanwhile, this strategy bypassed the use of an oxidant as well as the harsh conditions normally necessary for transition-metal-mediated arene C-H coupling with arylboronic acids as an arylation counterpart, offering a straightforward alternative to access optically active biaryls.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32017378

RESUMO

N-arylcarbazole structures are important because of their prevalence in natural products and functional OLED materials. C-H amination of arenes has been widely recognized as the most efficient approach to access these structures. Conventional strategies involving transition-metal catalysts suffer from confined substrate generality and the requirement of exogenous oxidants. Organocatalytic enantioselective C-N chiral axis construction remains elusive. Presented here is the first organocatalytic strategy for the synthesis of novel axially chiral N-arylcarbazole frameworks by the assembly of azonaphthalenes and carbazoles. This reaction accommodates broad substrate scope and gives atropisomeric N-arylcarbazoles in good yields with excellent enantiocontrol. This approach not only offers an alternative to metal-catalyzed C-N cross-coupling, but also brings about opportunities for the exploitation of structurally diverse N-aryl atropisomers and OLED materials.

6.
Genet Test Mol Biomarkers ; 23(12): 829-836, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31821092

RESUMO

Background: Pancreatic adenocarcinoma (PAAD) is an aggressive and invasive tumor with poor prognosis. Identifying prognostic biomarkers of PAAD will provide crucial information for developing treatment plans. Methods: In this analysis, a gene-expression dataset, containing RNA-sequencing data recalculated into transcripts per million, was obtained from the UCSC Xena platform. Three thousand nine hundred and seventy six differentially expressed genes were obtained with analysis of variance. Using these data a co-expression network was constructed using weighted gene co-expression network analysis, from which we obtained eight modules. Results: The blue module included 497 genes and demonstrated significant negative correlation with overall survival. Furthermore, pathway analyses demonstrated the involvement of many of these genes in the tight junction pathway, which plays a critical role in PAAD. In addition, we identified six genes in common (i.e., ANXA2 [annexin A2], EPHA2 [erythropoietin-producing hepatocellular class A2], ITGB4 [integrin beta 4], KRT19 [keratin type I cytoskeletal 19], LGALS3 [galectin-3], and S100A14 [S100 calcium binding protein A14]) between the protein-protein interaction and gene co-expression networks that may have critical functions in PAAD. These hub genes were not only highly expressed at the RNA level but also exhibited high expression in the immunohistological data in the Human Protein Atlas Database. Conclusion: Thus, this research clarified the framework of co-expressed gene modules in PAAD and highlighted potential prognostic biomarkers for the clinical diagnosis of PAAD.


Assuntos
Neoplasias Pancreáticas/genética , Junções Íntimas/genética , Adenocarcinoma/genética , Adenocarcinoma/metabolismo , Biomarcadores Tumorais/genética , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica/genética , Redes Reguladoras de Genes/genética , Humanos , Neoplasias Pancreáticas/metabolismo , Prognóstico , RNA/genética , Junções Íntimas/metabolismo , Transcriptoma/genética
7.
BMC Genomics ; 20(1): 661, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31426741

RESUMO

BACKGROUND: Long non-coding RNAs (LncRNAs) are part of genes, which are not translated into proteins and play a vital role in plant growth and development. Nevertheless, the presence of LncRNAs and how they functions in Ligon-lintless-1 mutant during the early cessation of cotton fiber development are still not well understood. In order to investigate the function of LncRNAs in cotton fiber development, it is necessary and important to identify LncRNAs and their potential roles in fiber cell development. RESULTS: In this work, we identified 18,333 LncRNAs, with the proportion of long intergenic noncoding RNAs (LincRNAs) (91.5%) and anti-sense LncRNAs (8.5%), all transcribed from Ligon-lintless-1 (Li1) and wild-type (WT). Expression differences were detected between Ligon-lintless-1 and wild-type at 0 and 8 DPA (day post anthesis). Pathway analysis and Gene Ontology based on differentially expressed LncRNAs on target genes, indicated fatty acid biosynthesis and fatty acid elongation being integral to lack of fiber in mutant cotton. The result of RNA-seq and RT-qPCR clearly singles out two potential LncRNAs, LNC_001237 and LNC_017085, to be highly down-regulated in the mutant cotton. The two LncRNAs were found to be destabilized or repressed by ghr-miR2950. Both RNA-seq analysis and RT-qPCR results in Ligon-lintless-1 mutant and wild-type may provide strong evidence of LNC_001237, LNC_017085 and ghr-miR2950 being integral molecular elements participating in various pathways of cotton fiber development. CONCLUSION: The results of this study provide fundamental evidence for the better understanding of LncRNAs regulatory role in the molecular pathways governing cotton fiber development. Further research on designing and transforming LncRNAs will help not only in the understanding of their functions but will also in the improvement of fiber quality.


Assuntos
Gossypium/crescimento & desenvolvimento , Gossypium/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Fibra de Algodão , Ácidos Graxos/metabolismo , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Gossypium/metabolismo , MicroRNAs/metabolismo , Mutação , RNA Mensageiro/metabolismo , RNA-Seq , Transdução de Sinais , Transcriptoma
8.
PeerJ ; 7: e6555, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30886771

RESUMO

Background: Adrenocortical carcinoma (ACC) is a rare and aggressive malignant cancer in the adrenal cortex with poor prognosis. Though previous research has attempted to elucidate the progression of ACC, its molecular mechanism remains poorly understood. Methods: Gene transcripts per million (TPM) data were downloaded from the UCSC Xena database, which included ACC (The Cancer Genome Atlas, n = 77) and normal samples (Genotype Tissue Expression, n = 128). We used weighted gene co-expression network analysis to identify gene connections. Overall survival (OS) was determined using the univariate Cox model. A protein-protein interaction (PPI) network was constructed by the search tool for the retrieval of interacting genes. Results: To determine the critical genes involved in ACC progression, we obtained 2,953 significantly differentially expressed genes and nine modules. Among them, the blue module demonstrated significant correlation with the "Stage" of ACC. Enrichment analysis revealed that genes in the blue module were mainly enriched in cell division, cell cycle, and DNA replication. Combined with the PPI and co-expression networks, we identified four hub genes (i.e., TOP2A, TTK, CHEK1, and CENPA) that were highly expressed in ACC and negatively correlated with OS. Thus, these identified genes may play important roles in the progression of ACC and serve as potential biomarkers for future diagnosis.

9.
Org Biomol Chem ; 17(9): 2351-2355, 2019 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-30758364

RESUMO

A series of chiral heterocyclic biaryls with a pyridyl moiety were prepared in moderate to good yields with up to 92% ee via asymmetric Suzuki-Miyaura coupling. The chiral-bridged biphenyl monophosphine ligand L1 was found to be much more effective in the reaction enantioselection than its counterpart binaphthyl monophosphine ligands.

10.
J BUON ; 23(2): 398-402, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29745083

RESUMO

PURPOSE: The purpose of this investigation was to determine the expression and significance of tumor associated macrophages (TAMs) and CXCR4 in non-small cell lung cancer (NSCLC). METHODS: Immunohistochemical staining was used to analyze the expression of CD68 (TAM surface marker) and CXCR4 in 68 cases of NSCLC and 17 cases of normal lung tissue. RESULTS AND CONCLUSION: The positive rate of CD68 was 66.2% (45/68) and CXCR4 was 61.8% (42/68) in the lung cancer tissues, while the rates in normal tissues were statistically significantly lower at 27.3% (3/17) and 11.8% (2/17), respectively. The tissue expressions of CD68 and CXCR4 in NSCLC tissues were significantly correlated with a higher TNM staging and lymph node metastasis (p<0.05); and the expression of CD68 was positively correlated with the expression of CXCR4 (r=0.461, p=0.000). The TAMs infiltration and the expression of CXCR4 were closely correlated with the cellular carcinogenesis and development of NSCLC and their combined detection could provide a potential target for clinical diagnosis and adjuvant therapy for patient with NSCLC.


Assuntos
Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Macrófagos/metabolismo , Receptores CXCR4/genética , Idoso , Biomarcadores Tumorais/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Metástase Linfática , Macrófagos/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Transdução de Sinais
11.
AIDS Care ; 29(12): 1524-1528, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28508665

RESUMO

We aimed to explore the prevalence of and risk factors for depressive symptoms (DS) among people living with HIV/AIDS (PLWHA) receiving antiretroviral treatment (ART) in Wuhan, Hubei, China. A cross-sectional study evaluating adult PLWHA receiving ART in nine designated clinical hospitals was conducted from October to December 2015. The validated Beck Depression Inventory (BDI) was used to assess DS in eligible participants. Socio-demographical, epidemiological and clinical data were directly extracted from the case reporting database of the China HIV/AIDS Information Network. Multinomial regression analysis was used to explore the risk factors for DS. 394 participants were finally included in all analyses. 40.3% were found to have DS with 13.7% having mild DS and 26.6% having moderate to severe DS. The results of multinomial regression analysis suggested that being married or living with a partner, recent experience of ART-related side effects, and/or history of HCV infection were positively associated with mild DS, while increasing age was positively associated with moderate to severe DS.


Assuntos
Terapia Antirretroviral de Alta Atividade/métodos , Depressão/epidemiologia , Infecções por HIV/tratamento farmacológico , Infecções por HIV/psicologia , Síndrome de Imunodeficiência Adquirida/epidemiologia , Adulto , China/epidemiologia , Estudos Transversais , Depressão/diagnóstico , Depressão/psicologia , Feminino , Infecções por HIV/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Escalas de Graduação Psiquiátrica , Fatores de Risco , Parceiros Sexuais
12.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 29(2): 219-221, 2017 Feb 22.
Artigo em Chinês | MEDLINE | ID: mdl-29469331

RESUMO

OBJECTIVE: To investigate the status of knowledge, attitude and behavior of schistosomiasis control of rural residents in Wanjiang River region after a flood, so as to provide the reference for targeted health education. METHODS: The multistage sampling was applied to select the respondents in rural residents in Wanjiang River region, and the self-designed questionnaire was used to investigate the current situation of knowledge, attitude and behavior of schistosomiasis prevention and control of the rural residents. RESULTS: The total awareness rate of knowledge about the prevention and control of schistosomiasis was 47.92%. The age, education, family income, relatives and friends with medical background, and health education significantly influenced the awareness rate (χ2 = 12.76, 89.19, 18.19, 50.83 and 92.60 respectively, all P < 0.05). The accuracy rates of attitude and behavior in schistosomiasis control were 62.89% and 52.37% respectively. CONCLUSIONS: The awareness rate of knowledge about the prevention and control of schistosomiasis, and the accuracy rates of attitude and behavior in schistosomiasis control of the rural residents in Wanjiang River region are all inefficient, and therefore, the targeted health education should be strengthened to decrease the risk of schistosomiasis transmission.


Assuntos
Inundações , Conhecimentos, Atitudes e Prática em Saúde , População Rural , Esquistossomose/prevenção & controle , China , Educação em Saúde , Humanos , Rios , Inquéritos e Questionários
13.
Arch Dermatol Res ; 307(9): 849-54, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26404084

RESUMO

Bullous pemphigoid (BP), a common autoimmune skin disease, is associated with IgG autoantibodies against the hemidesmosomal proteins, BP180 and BP230. In addition to IgG, IgE has been shown to play a role in the disease. However, the association between disease activity and IgE specific to the NC16A domain of BP180 in blister fluid remains unclear. Our objective was to evaluate the correlation between BP disease activity and BP180 NC16A-specific IgE sera and blister fluid titers, and to analyze changes during treatment. We evaluated the levels of anti-BP180 IgE autoantibodies in the sera and blister fluids of 37 BP patients using an Enzyme-linked immunosorbent assay. We also observed changes in the levels of these antibodies in 2 BP patients at 4 or 5 different time points (day 0 when the patient first visited our hospital, day 5, day 14, day 39 and day 62 for patient 1; day 0, day 4, day 8 and day 17 for patient 2). We also collected extra serum samples from the 2 patients when the disease was controlled (blister disappeared) (day 85, day 104 and day 146 for patient 1 and day 123, day 158 and day 189 for the other patient). IgE anti-BP180 antibodies were detected in the serum of 72.97 % of the patients. There was no correlation between disease activity scores and BP180 NC16A IgE titers in serum (r = -0.077, p > 0.05) or in blister fluid (r = 0.262, p > 0.05). The levels of the autoantibody in serum were positively correlated with that in blister fluid (r = 0.6651, p < 0.001); however, the levels continued to rise despite effective control of the disease in the initial two to 6 weeks of diagnosis. The level of this autoantibody reached a peak on day 39 for patient 1 and on day 17 for patient 2 although the systemic and topical medication of steroid had controlled the disease process effectively. We conclude that levels of anti-BP180 NC16A IgE are higher in the sera than blister fluids. These levels could generally reflect disease severity throughout the course of the disease, but not in the early stages of medication.


Assuntos
Anticorpos/sangue , Anticorpos/imunologia , Autoantígenos/imunologia , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Colágenos não Fibrilares/imunologia , Penfigoide Bolhoso/imunologia , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/sangue , Autoimunidade/imunologia , Vesícula/imunologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Masculino , Pessoa de Meia-Idade , Penfigoide Bolhoso/patologia , Estrutura Terciária de Proteína
14.
J Org Chem ; 80(14): 6968-75, 2015 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-26105579

RESUMO

A series of novel chiral phosphoramidite ligands based on chiral-bridged biphenyl backbones have been prepared conveniently and characterized. The ligands complexed with [IrCodCl]2 provided the first iridium catalyst system for the asymmetric addition of arylboronic acids to N-protected isatins with high efficiency. When performed in THF/H2O at 80 °C with 2 equiv of the arylboronic acids, the transformations acquired good to excellent results (up to 98% yield and 95% ee).


Assuntos
Ácidos Borônicos/química , Isatina/química , Compostos Organofosforados/síntese química , Catálise , Hidrogenação , Irídio , Ligantes , Estrutura Molecular , Compostos Organofosforados/química
15.
Eur J Radiol ; 84(7): 1293-8, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25982461

RESUMO

OBJECTIVE: To discuss the value of (18)F-fluorodeoxyglucose-positron emission tomography ((18)F-PET/CT) scans in the diagnosis and early staging of non-mycosis fungoides/Sézary's syndrome cutaneous malignant lymphomas (non-MF/SS CML). MATERIALS AND METHODS: A total of 18 cases with non-MF/SS CML, confirmed by pathology or on clinical grounds, were analyzed in this study. The sensitivity of CT and PET/CT scans in the diagnosis of primary skin lesions, as well as the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of CT and PET/CT scans in the diagnosis of lymph nodes (LNs) and other organs (except skin and LNs) were calculated. RESULTS: The diagnostic sensitivity of CT and PET/CT scans in the diagnosis of primary skin lesions was 82.4% (14/17) and 100% (17/17), respectively. The diagnostic sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of CT and PET/CT scans in the diagnosis of LN lesions were 55.6% (5/9), 88.9% (8/9), 72.2% (13/18), 83.3% (5/6), 66.7% (8/12), and 88.9% (8/9), 100% (9/9), 94.4% (17/18), 100% (8/8), 90.0% (9/10), respectively. The diagnostic value of the CT and PET/CT scans in the diagnosis of involvement of other organs, were 40.4% (2/5), 100% (13/13), 83.3 (15/18), 100% (2/2), 81.3% (13/16) and 80.6% (4/5), 100% (13/13), 94.4% (17/18), 100% (3/3), 92.9% (13/14), respectively. CONCLUSIONS: (18)F-FDG PET/CT has high value in the diagnosis and early staging of non-MF/SS CMLs.


Assuntos
Fluordesoxiglucose F18 , Linfoma/diagnóstico , Tomografia por Emissão de Pósitrons/métodos , Síndrome de Sézary/diagnóstico , Neoplasias Cutâneas/diagnóstico , Tomografia Computadorizada por Raios X/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Linfonodos/diagnóstico por imagem , Linfonodos/patologia , Linfoma/patologia , Masculino , Pessoa de Meia-Idade , Imagem Multimodal/métodos , Micose Fungoide , Estadiamento de Neoplasias , Compostos Radiofarmacêuticos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Síndrome de Sézary/patologia , Pele/diagnóstico por imagem , Neoplasias Cutâneas/patologia , Adulto Jovem
16.
J Altern Complement Med ; 20(9): 732-5, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25046151

RESUMO

UNLABELLED: Abstract Objective: A case series is presented to investigate the efficacy and safety of Erhegao for patients with breast cancer who have radiotherapy-induced moist desquamation. METHODS: Eighteen women with breast cancer who received radiotherapy and developed moist desquamation were enrolled. Erhegao cream, a Traditional Chinese Medicine formula consisting of zinc oxide powder, calamine powder, and lithospermum oil, was applied on areas of moist desquamation. Application was repeated once a day until healing. The primary end point for efficacy was the time to healing of the moist desquamation areas. A numerical rating scale was used to measure wound pain relief daily. Incidence of toxicity was also assessed. RESULTS: The average time to healing of the moist desquamation area was 13.56 days. The mean pain scores on the first, third, and seventh days were 5.22, 2.94, and 0.83, respectively. Eight-three percent of patients reported pain relief after the first 3 days, and 94%, after the first week. The mean daily reduction in the pain score was 0.40. None of the patients developed clinical infections or reported any toxicity. CONCLUSIONS: This formula is effective and safe, especially for pain relief, and may be an alternative treatment for radiotherapy-induced moist desquamation in patients with breast cancer. Future randomized, controlled studies are needed to better evaluate the efficacy of Erhegao cream.


Assuntos
Neoplasias da Mama/radioterapia , Medicamentos de Ervas Chinesas/uso terapêutico , Dor/tratamento farmacológico , Lesões por Radiação/tratamento farmacológico , Dermatopatias/tratamento farmacológico , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Adulto , Idoso , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Humanos , Lithospermum , Medicina Tradicional Chinesa , Pessoa de Meia-Idade , Medição da Dor , Projetos Piloto , Óleos Vegetais/farmacologia , Óleos Vegetais/uso terapêutico , Lesões por Radiação/etiologia , Pele/patologia , Creme para a Pele , Dermatopatias/etiologia , Óxido de Zinco/farmacologia , Óxido de Zinco/uso terapêutico
17.
Pak J Med Sci ; 29(3): 762-7, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-24353624

RESUMO

OBJECTIVE: Polymorphisms in XPG were considered to contribute to the clinical outcome of patients receiving platinum drug chemotherapy. We investigated the impact of several potential SNPs of XPG on the efficacy of platinum-based chemotherapy in NSCLC patients. METHODS: A total of 433 patients were consecutively selected between Nov. 2006 and Dec. 2007, and were followed-up up to Nov. 2011. The genotyping of six SNPs (rs2296147, rs751402, rs873601, rs4150375, rs17655 and rs2094258) were genotyped using the Taqman real-time PCR method with a 7900 HT sequence detector system. RESULTS: Patients carrying CT+TT genotype of rs2296147 had a significantly longer median PFS (17.5 months) and OS (26.8 months) than CC genotype. Hazard ratio (HR) for PFS and OS in patients with CT+TT genotype of rs2296147 was respectively 0.73(0.51-0.97) and 0.66(0.48-0.99) when compare CC genotype, respectively. Similarly, patients with rs2094258 AG+GG genotype had a longer median progression time (18.4 months) and overall survival time (27.3 months) when compared with those with AA genotype, and HRs(95% CI) for PFS and OS were 0.44(0.34-0.78) and 0.51(0.39-0.82), respectively. CONCLUSIONS: Our study suggests rs2296147 CT+TT and rs2094258 AG+GG genotypes contribute to the better survival of NSCLC. Our study provides significant information on role of prognostic value of XPG SNPs, and detecting of XPG could be used as predictive markers toward individualizing NSCLC treatment strategies.

18.
Biosystems ; 114(3): 214-8, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24035832

RESUMO

The inflammatory response is a rapid and complex physiological reaction to infection, which must be carefully modulated to remove pathogens and prevent the consequences of unregulated expression including cancer. MiRNAs are small noncoding RNAs that regulate protein-coding genes via post-transcriptional repression. Emerging evidence suggests that the role of miRNAs in the regulation of immune responses as well as inflammatory networks in various cell and tissue types. Here, we have constructed a mathematical model that integrates miR-21 and miR-146 expression into a signaling pathway to generate an in silico model for the process of inflammation. The results show that the negative feedback provided by miR-21 stimulates the propensity of oscillations in NF-κB and IL-6 activity, while the negative feedback provided by miR-146 dampens the oscillations of NF-κB and IL-6. This process is somewhat sensitive to the inputs of miR-21 and miR-146, suggesting that variations in the relative strength of the two feedbacks may provide for altered response dynamics to the same stimulus. Our findings reveal a novel regulatory module of two miRNA-mediated negative feedback loops that allows for the fine-tuning of the dynamics of key mediators in inflammation.


Assuntos
Redes Reguladoras de Genes/imunologia , Inflamação/imunologia , Interleucina-6/imunologia , MicroRNAs/imunologia , Modelos Imunológicos , NF-kappa B/imunologia , Transdução de Sinais/imunologia , Simulação por Computador , Redes Reguladoras de Genes/genética , Humanos , Interleucina-6/metabolismo , MicroRNAs/metabolismo , NF-kappa B/metabolismo , Transdução de Sinais/genética
19.
Eur J Radiol ; 82(9): 1546-51, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23726473

RESUMO

OBJECTIVE: This study aims to investigate the clinical performance of three-dimensional (3D) fast-recovery fast spin-echo (FRFSE) magnetic resonance dacryocystography (MRD) with topical administration of sterile saline solution for the assessment of the lacrimal drainage system (LDS). METHODS: A total of 13 healthy volunteers underwent both 3D-FRFSE MRD and two-dimensional (2D)-impulse recovery (IR)-single-shot fast spin-echo (SSFSE) MRD after topical administration of sterile saline solution, and 31 patients affected by primary LDS outflow impairment or postsurgical recurrent epiphora underwent 3D-FRFSE MRD and conventional T1- and T2-weighted sequences. All patients underwent lacrimal endoscopy or surgery, which served as a standard of reference for confirming the MRD findings. RESULTS: 3D-FRFSE MRD detected more visualized superior and inferior canaliculi and nasolacrimal duct than 2D-IR-SSFSE MRD. Compared with 2D-IR-SSFSE MRD, 3D-FRFSE MRD showed more visualized segments per LDS, although the difference was not statistically significant. Significant improvements in the inferior canaliculus and nasolacrimal duct visibility grades were achieved using 3D-FRFSE MRD. 3D-FRFSE MRD had 100% sensitivity and 63.6% specificity for detecting LDS obstruction. In 51 out of the 62 LDSs that were assessed, a 90% agreement was noted between the findings of 3D-FRFSE MRD and lacrimal endoscopy in detecting the obstruction level. CONCLUSION: 3D-FRFSE MRD combined with topical administration of sterile saline solution is a simple and noninvasive method of obtaining detailed morphological and functional information on the LDS. Overall, 3D-FRFSE MRD could be used as a reliable diagnostic method in many patients with epiphora prior to surgery.


Assuntos
Imagem Ecoplanar/métodos , Aumento da Imagem/métodos , Imageamento Tridimensional/métodos , Doenças do Aparelho Lacrimal/patologia , Aparelho Lacrimal/patologia , Cloreto de Sódio/administração & dosagem , Administração Tópica , Adulto , Algoritmos , Anisotropia , Meios de Contraste/administração & dosagem , Feminino , Humanos , Interpretação de Imagem Assistida por Computador/métodos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Marcadores de Spin , Esterilização , Adulto Jovem
20.
Eur J Pediatr ; 172(9): 1167-71, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23636285

RESUMO

UNLABELLED: The present study examined the association between body mass index (BMI) and skinfold thickness (SFT) with blood pressure (BP) in 12-year-old children in Shandong, China. A total of 920 (464 boys and 456 girls) 12-year-old students participated in this study. All subjects were divided into four groups (BMI < 25th, 25th ≤ BMI < 50th, 50th ≤ BMI < 75th, and BMI ≥ 75th) according to the percentile of BMI and into four groups (SFT < 25th, 25th ≤ SFT < 50th, 50th ≤ SFT < 75th, and SFT ≥ 75th) according to the percentile of SFT, respectively. Comparisons of BP among different groups were made by one-way ANOVA. High BP status was defined as systolic blood pressure (SBP) ≥ 95th and/or diastolic blood pressure (DBP) ≥ 95th percentile for age and gender. BMI and SFT were all significantly (P < 0.001) and positively related to SBP and DBP in both boys and girls. The prevalence of high BP in each group is rising with the percentiles of BMI and SFT in both boys and girls. CONCLUSION: There is a strong positive relationship between BMI, SFT, and BP in 12-year-old children; the present findings emphasize the importance of preventing excess BMI and SFT in order to prevent future-related problems such as hypertension in children and adolescents.


Assuntos
Pressão Sanguínea/fisiologia , Índice de Massa Corporal , Hipertensão/etiologia , Obesidade/complicações , Pregas Cutâneas , Determinação da Pressão Arterial , Criança , Feminino , Humanos , Hipertensão/diagnóstico , Masculino , Obesidade/diagnóstico , Sobrepeso/complicações , Sobrepeso/diagnóstico
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