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1.
Sci China Life Sci ; 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33655434

RESUMO

African swine fever virus (ASFV) has been circulating in China for more than two years, and it is not clear whether the biological properties of the virus have changed. Here, we report on our surveillance of ASFVs in seven provinces of China, from June to December, 2020. A total of 22 viruses were isolated and characterized as genotype II ASFVs, with mutations, deletions, insertions, or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018 (HLJ/18), the earliest isolate in China. Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a non-hemadsorbing (non-HAD) phenotype. Four isolates were tested for virulence in pigs; two were found to be as highly lethal as HLJ/18. However, two non-HAD isolates showed lower virulence but were highly transmissible; infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease, whereas 103 TCID50 dose caused non-lethal, sub-acute or chronic disease, and persistent infection. The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control.

2.
Global Health ; 17(1): 23, 2021 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-33632259

RESUMO

OBJECTIVES: The psychological distress caused by COVID-19 may be pronounced among the parents of children with autism spectrum disorder (ASD). This study aimed to investigate psychological distress among parents of children with ASD during the COVID-19 pandemic. METHODS: A total of 1764 parents of children with ASD and 4962 parents of typically developing (TD) children were recruited. The participants completed an online survey which contained demographic information, the impact due to COVID-19 crisis, resilience, coping styles, anxiety and depression. Hierarchical linear regression was used to assess the contributions of these variables to anxiety and depression. RESULTS: After adjusting for demographic variables, the following factors were associated with parents' anxiety and depression symptoms: (i) Whether or not the participants had a child with ASD; (ii) resilience; (iii) coping strategies, and; (iv) the impact due to COVID-19. Among these, the psychological stress caused by COVID-19 played the most important role in parental anxiety (ß = 0.353) and depression (ß = 0.242) symptoms. Parents of children with ASD had lower levels of resilience and positive coping, and used more negative coping strategies than parents of TD children. Among all participants, 8.0 and 24.2% of parents had symptoms of anxiety and depression, respectively. Compared to parents of TD children, more parents of children with ASD exhibited symptoms of anxiety and depression (12.2% vs. 6.6%; 31.0% vs. 21.7%, respectively). CONCLUSIONS: During the COVID-19 pandemic, parents experienced varying levels of anxiety and depression, particularly, parents of children with ASD. More specific attention should be paid to parental mental health and long-term effective intervention programs, that are targeted towards parents of children with ASD, and such programs should be promoted around China in the wake of the COVID-19 crisis.


Assuntos
Transtorno do Espectro Autista , Pais/psicologia , Angústia Psicológica , Adolescente , Adulto , Ansiedade/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Depressão/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Adulto Jovem
3.
Open Biol ; 11(2): 200306, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33529552

RESUMO

Autism spectrum disorder (ASD) is a group of developmental disabilities, the aetiology of which remains elusive. The endocannabinoid (eCB) system modulates neurotransmission and neuronal plasticity. Evidence points to the involvement of this neuromodulatory system in the pathophysiology of ASD. We investigated whether there is a disruption to the eCB system in ASD and whether pharmacological modulation of the eCB system might offer therapeutic potential. We examined three major components of the eCB system-endogenous cannabinoids, their receptors and associated enzymes-in ASD children as well as in the valproic acid (VPA) induced animal model in autism. Furthermore, we specifically increased 2-arachidonoylglycerol (2-AG) levels by administering JZL184, a selective inhibitor of monoacylglycerol lipase which is the hydrolytic enzyme for 2-AG, to examine ASD-like behaviours in VPA-induced rats. Results showed that autistic children and VPA-induced rats exhibited reduced eCB content, increased degradation of enzymes and upregulation of CBRs. We found that repetitive and stereotypical behaviours, hyperactivity, sociability, social preference and cognitive functioning improved after acute and chronic JZL184 treatment. The major efficacy of JZL184 was observed after administration of a dosage regimen of 3 mg kg-1, which affected both the eCB system and ASD-like behaviours. In conclusion, a reduced eCB signalling was observed in autistic children and in the ASD animal model, and boosting 2-AG could ameliorate ASD-like phenotypes in animals. Collectively, the results suggested a novel approach to ASD treatment.

4.
Mol Med Rep ; 23(4): 1, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33537829

RESUMO

Globally, thyroid cancer (TC) is considered to be the commonest endocrine malignancy. GINS complex subunit 2 (GINS2) belongs to the GINS complex family and is associated with cellular migration, invasion and growth. The present study aimed to investigate the underlying mechanisms of GINS2 on cell viability, migration and invasion in TC cells. By using MTT, wound healing and Transwell assays, the cell viability, migration and invasion were determined. Apoptosis was examined by immunofluorescence. Western blotting was used to detect protein expression levels. In the present study, biological function analysis demonstrated that GINS2 interference attenuated cell viability, migration and invasion in TC cell lines (K1 and SW579). It was discovered that, compared with the control group, GINS2 silencing induced apoptosis in TC cells. Additionally, GINS2 interference inhibited key proteins in the MAPK signaling pathway, including JNK, ERK and p38. According to these comparative experiments, GINS2 was considered to act a pivotal part in cell viability, migration and invasion of TC by regulating the MAPK signaling pathway and might be a potential therapeutic target for treating TC.

5.
Ecotoxicol Environ Saf ; 213: 112037, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33609998

RESUMO

OBJECTIVE: Telomere length (TL) is a biomarker for biological aging, and the initial setting of TL at birth is a determinant factor of TL in later life. Newborn TL is sensitive to maternal metals concentrations, while study about the association between maternal manganese (Mn) concentrations and newborn TL was not found. Our study aimed to investigate whether newborn TL is related to maternal Mn concentrations. METHODS: Data were collected from a birth cohort study of 762 mother-newborn pairs conducted from November 2013 to March 2015 in Wuhan, China. We measured the Mn concentrations in spot urine samples collected during three trimesters by inductively coupled plasma mass spectrometry (ICP-MS) and relative cord blood TL by quantitative real-time polymerase chain reaction (qPCR). We applied multiple informant models to investigate the associations between maternal Mn concentrations and cord blood TL. RESULTS: The geometric mean of creatinine-corrected urinary Mn concentrations were 1.58 µg/g creatinine, 2.53 µg/g creatinine, and 2.62 µg/g creatinine in the first, second, and third trimester, respectively. After adjusting for potential confounders, a doubling of maternal urinary Mn concentration during the second trimester was related to a 2.10% (95% CI: 0.25%, 3.99%) increase in cord blood TL. Mothers with the highest tertile of urinary Mn concentrations during the second trimester had a 9.67% (95% CI: 2.13%, 17.78%) longer cord blood TL than those with the lowest tertile. This association was more evident in male infants. No relationship was found between maternal urinary Mn concentrations and cord blood TL during the first and third trimesters in our study. CONCLUSIONS: Our findings suggested that maternal Mn concentration during the second trimester was positively associated with newborn TL. These results might provide an epidemiology evidence on the protective role of maternal Mn for newborn TL and offer clues for the early prevention of telomere shortening related diseases.

6.
Metab Brain Dis ; 36(4): 589-599, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33555497

RESUMO

BACKGROUND: Carbonic anhydrase II deficiency syndrome is an autosomal recessive osteopetrosis with renal tubular acidosis and cerebral calcifications. We tried to detect the causative mutation for carbonic anhydrase II deficiency syndrome in a five-generation Chinese family. MATERIALS AND METHODS: Genomic DNA was extracted from whole blood of the proband, his grandmother, parents, aunt, uncle and sister. The exomes were sequenced by whole exon sequencing followed by genetic analysis and Sanger sequencing validation. Then, physical and chemical properties studies and structure analysis were performed on mutated protein. Finally, Minigene model of vector plasmids for wild type and mutant type was constructed and transfected into human embryonic kidney 293T cells to further explore the expression change of CA2 transcript and protein after mutation. RESULTS: Sequencing and genetic analysis have revealed the homozygous nonsense mutation of CA2 gene (c.368G > A, p.W123X) in the exon 4 of chromosome 8 of the proband, while it was not found in his grandmother, parents, aunt, uncle and sister. Furthermore, Sanger sequencing in the proband and his parents validated the mutation. Properties and structure of mutated CA2 proteins changed after mutation, especially in change of protein modification and hindrance of zinc ions binding, which may lead to decreased protein expression level of CA2. CONCLUSIONS: We found a new homozygous nonsense mutation in CA2 gene (c.368G > A, p.W123X), which may be valuable in the early diagnosis and therapy of carbonic anhydrase II deficiency syndrome.

7.
IEEE Trans Cybern ; PP2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33635814

RESUMO

Despite the promising preliminary results, tensor-singular value decomposition (t-SVD)-based multiview subspace is incapable of dealing with real problems, such as noise and illumination changes. The major reason is that tensor-nuclear norm minimization (TNNM) used in t-SVD regularizes each singular value equally, which does not make sense in matrix completion and coefficient matrix learning. In this case, the singular values represent different perspectives and should be treated differently. To well exploit the significant difference between singular values, we study the weighted tensor Schatten p-norm based on t-SVD and develop an efficient algorithm to solve the weighted tensor Schatten p-norm minimization (WTSNM) problem. After that, applying WTSNM to learn the coefficient matrix in multiview subspace clustering, we present a novel multiview clustering method by integrating coefficient matrix learning and spectral clustering into a unified framework. The learned coefficient matrix well exploits both the cluster structure and high-order information embedded in multiview views. The extensive experiments indicate the efficiency of our method in six metrics.

8.
Inorg Chem ; 2021 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33570916

RESUMO

All-inorganic halide perovskite (CsPb2Br5) nanocrystals (NCs) have received widespread attention owing to their unique photoelectric properties. This work reports a novel strategy to control the phase transition from CsPbBr3 to CsPb2Br5 and investigates the effects of different treatment times and treatment temperatures on perovskite NCs formation. By controlling the volume of tetraethoxysilane (TEOS) added, the formation of different phases of perovskite powder can be well controlled. In addition, a white light-emitting diode (WLED) device is designed by coupling the CsPbBr3/CsPbBr3-CsPb2Br5 NCs@TEOS nanocomposite and CaAlSiN3:Eu2+ commercial phosphor with a 460 nm InGaN blue chip, exhibiting a high luminous efficiency of 57.65 lm/W, color rendering index (CRI) of 91, and a low CCT of 5334 K. The CIE chromaticity coordinates are (0.3363, 0.3419). This work provides a new strategy for the synthesis of CsPbBr3/CsPbBr3-CsPb2Br5 NCs@TEOS nanocomposite, which can be applied to the field of WLEDs and display devices.

9.
Hypertension ; : HYPERTENSIONAHA12015608, 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33611939

RESUMO

Ambient fine particulate matter (PM2.5) exposure during pregnancy was associated with hypertensive disorders of pregnancy (HDP) in previous studies, however, the results were inconsistent. In addition, its effects on blood pressure (BP) pattern, which was different between normal pregnancy and pregnancy with HDP, remain unclear. In this study, we estimate daily PM2.5 exposure levels of 7658 pregnant women from a prospective cohort study in Wuhan, China, using land use regression model. BP was repeatedly measured in each trimester. Linear mixed-effect model was used to examine associations of PM2.5 exposure with BP patterns during pregnancy. The association between PM2.5 exposure and HDP was estimated by modified Poisson regression. In the lowest quartile of PM2.5 exposure, BP fell gradually during early pregnancy and increase subsequently after a nadir at mid-pregnancy. In higher quartiles of PM2.5 exposure, a slower drop of BP was observed during early pregnancy, and the mid-pregnancy drop was less obvious compared with the lowest quartile. PM2.5 exposure during the second trimester was positively associated with the risk of HDP (relative risk =1.42 [95% CI, 1.19-1.70]), especially among women with excessive gestational weight gain (relative risk =1.48 [95% CI, 1.16-1.90; P for interaction =0.03). Our study suggests that PM2.5 exposure might play an important role in BP pattern during pregnancy and might increase the risk of HDP, especially among women with excessive gestational weight gain.

10.
Sci Rep ; 11(1): 1454, 2021 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33446901

RESUMO

Tramadol is an opioid used as an analgesic for treating moderate or severe pain. The long-term use of tramadol can induce several adverse effects. The toxicological mechanism of tramadol abuse is unclear. Limited literature available indicates the change of proteomic profile after chronic exposure to tramadol. In this study, we analyzed the proteomic and metabolomic profile by TMT-labeled quantitative proteomics and untargeted metabolomics between the tramadol and the control group. Proteomic analysis revealed 31 differential expressed serum proteins (9 increased and 22 decreased) in tramadol-treated mice (oral, 50 mg/kg, 5 weeks) as compared with the control ones. Bioinformatics analysis showed that the dysregulated proteins mainly included: enzyme inhibitor-associated proteins (i.e. apolipoprotein C-III (Apoc-III), alpha-1-antitrypsin 1-2 (Serpina 1b), apolipoprotein C-II (Apoc-II), plasma protease C1 inhibitor, inter-alpha-trypsin inhibitor heavy chain H3 (itih3)); mitochondria-related proteins (i.e. 14-3-3 protein zeta/delta (YWHAZ)); cytoskeleton proteins (i.e. tubulin alpha-4A chain (TUBA4A), vinculin (Vcl)). And we found that the differential expressed proteins mainly involved in the pathway of the protein digestion and absorption. Metabolomics analysis revealed that differential expressed metabolites mainly involved in protein ingestion and absorption, fatty acid biosynthesis, steroid hormone biosynthesis and bile secretion. Our overall findings revealed that chronic exposure to tramadol changed the proteomic and metabolomic profile of mice. Moreover, integrated proteomic and metabolomic revealed that the protein digestion and absorption is the common enrichment KEGG pathway. Thus, the combination of proteomics and metabolomics opens new avenues for the research of the molecular mechanisms of tramadol toxicity.

11.
Adv Mater ; : e2006577, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33470466

RESUMO

The development of all-solid-state Li metal batteries (ASSLMBs) has attracted significant attention due to their potential to maximize energy density and improved safety compared to the conventional liquid-electrolyte-based Li-ion batteries. However, it is very challenging to fabricate an ideal solid-state electrolyte (SSE) that simultaneously possesses high ionic conductivity, excellent air-stability, and good Li metal compatibility. Herein, a new glass-ceramic Li3.2 P0.8 Sn0.2 S4 (gc-Li3.2 P0.8 Sn0.2 S4 ) SSE is synthesized to satisfy the aforementioned requirements, enabling high-performance ASSLMBs at room temperature (RT). Compared with the conventional Li3 PS4 glass-ceramics, the present gc-Li3.2 P0.8 Sn0.2 S4 SSE with 12% amorphous content has an enlarged unit cell and a high Li+ ion concentration, which leads to 6.2-times higher ionic conductivity (1.21 × 10-3 S cm-1 at RT) after a simple cold sintering process. The (P/Sn)S4 tetrahedron inside the gc-Li3.2 P0.8 Sn0.2 S4 SSE is verified to show a strong resistance toward reaction with H2 O in 5%-humidity air, demonstrating excellent air-stability. Moreover, the gc-Li3.2 P0.8 Sn0.2 S4 SSE triggers the formation of Li-Sn alloys at the Li/SSE interface, serving as an essential component to stabilize the interface and deliver good electrochemical performance in both symmetric and full cells. The discovery of this gc-Li3.2 P0.8 Sn0.2 S4 superionic conductor enriches the choice of advanced SSEs and accelerates the commercialization of ASSLMBs.

12.
Public Health Nutr ; : 1-8, 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33472715

RESUMO

OBJECTIVE: Inflammation has been suggested to play an important role in the development and progression of metabolic syndrome (MetS). Dietary inflammatory index (DII), a measurement of inflammatory potential in diets, was suggested to be associated with MetS. The aim of this systematic review and meta-analysis was to establish the associations of DII with MetS and its components based on available observational studies. DESIGN: Systematic review and meta-analysis. SETTING: A comprehensive literature search of studies that assessed the associations between DII and MetS was conducted in PubMed, Medline and Embase, using a combination of search terms relating to DII and MetS. PARTICIPANTS: Eighteen articles were eligible, of which fourteen were cross-sectional and four were cohort in design. RESULTS: Results from the random effects meta-analysis showed significantly positive associations of higher DII (top v. bottom quartiles) with MetS (OR: 1·23 (95 % CI 1·10, 1·37)), abdominal obesity (OR: 1·15 (95 % CI 1·02, 1·29)), high blood pressure (OR: 1·17 (95 % CI 1·07, 1·29)), hyperglycaemia (OR: 1·18 (95 % CI 1·05, 1·33)) and hypertriacylglycerolaemia (OR: 1·17 (95 % CI 1·07, 1·28)). The effects of summary OR became stronger when analyses were restricted to cohorts, studies that adjudged for covariates (including BMI, physical activity and total energy intake). CONCLUSIONS: Higher DII, representing pro-inflammatory diet, is associated with higher odds of MetS and its components, except for low HDL-cholesterol. The findings prompt dietary interventions for preventing MetS from the aspect of inflammation.

13.
Appl Microbiol Biotechnol ; 105(4): 1709-1720, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33512573

RESUMO

Biological ethylene production is a promising sustainable alternative approach for fossil-based ethylene production. The high glucose utilization of Z. mobilis makes it as a promising bioethylene producer. In this study, Zymomonas mobilis has been engineered to produce ethylene through the introduction of the synthetic ethylene-forming enzyme (EFE). We also investigated the effect of systematically knocking out the competitive metabolic pathway of pyruvate in an effort to improve the availability of pyruvate for ethylene production in Z. mobilis expressing EFE. Guided by these results, we tested a number of conjectures that could improve the α-ketoglutarate supply. Optimization of these pathways and different substrate supplies resulted in a greater production of ethylene (from 1.36 to 12.83 nmol/OD600/mL), which may guide future engineering work on ethylene production using other organisms. Meanwhile, we achieved an ethylene production of 5.8 nmol/OD600/mL in the ZM532-efe strain using enzymatic straw hydrolysate of corn straw as the sole carbon source. As a preferred host in biorefinery technologies using lignocellulosic biomass as feedstock, heterologous expression of EFE in Z. mobilis converts the non-ethylene producing strain into an ethylene-producing one using a metabolic engineering approach, which is of great significance for the utilization of cellulosic biomass in the future. KEY POINTS: • Heterologous expression of EFE in Z. mobilis successfully converted the non-ethylene producing strain into an ethylene producer (1.36 nmol/OD600/mL). Targeted modifications of the central carbon metabolism can effectively improve ethylene production (peak production: 8.3 nmol/OD600/mL). • The addition of nutrients to the medium can further increase the production of ethylene (peak production: 12.8 nmol/OD600/mL). • The ZM532-efe strain achieved an ethylene production of 5.8 nmol/OD600/mL when enzymatic hydrolysate of corn straw was used as the sole carbon source.

14.
Environ Int ; 148: 106374, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33476788

RESUMO

BACKGROUND: The association between exposure to organochlorine pesticides (OCPs) and infant growth has been reported contradictorily in previous studies. Few studies have investigated the effects of prenatal exposure to OCPs on infant growth assessed longitudinally at multiple time points. OBJECTIVES: The purpose of the study was to examine the associations between prenatal exposure to OCPs and infant growth at birth, 6, 12 and 24 months of age, and further to explore the potential sex-specific effects. METHODS: The study population included 1039 mother-infant pairs who participated in a birth cohort study in Wuhan, China. The weight, length and body mass index (BMI) z-score of infants were measured and calculated at birth, 6, 12 and 24 months of age. The overweight status was defined as BMI z-score ≥ 85th percentile according to the standard of World Health Organization. The concentrations of OCPs were measured in cord serum, including hexachlorocyclohexanes (HCHs, consisted of α-HCH, ß-HCH, and γ-HCH), p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) and its metabolites: p,p'-dichlorodiphenyldichloroethane (p,p'-DDD), and p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE). Generalized linear models were applied to estimate the associations of cord OCPs with infant growth parameters. A group-based semiparametric mixture model was used to estimate growth patterns of infants. Linear-mixed growth curve models were used to examine relationships between predicted growth trajectories and prenatal exposure to OCPs. Weighted quantile sum regression (WQSR) analyses were used to estimate the mixture effects of OCPs on infant growth. RESULTS: Higher cord serum ß-HCH concentrations were associated with higher BMI z-score at 12 [ß = 0.07, 95%CI: 0.01, 0.13] and 24 months of age [ß = 0.08, 95% CI: 0.02, 0.14]. Similar patterns were observed for relationships of γ-HCH [ß = 0.04, 95%CI: 0.01, 0.07] and p,p'-DDT [ß = 0.03, 95% CI: 0.00, 0.06] with BMI z-score at 6 and 12 months of age, respectively. However, higher cord serum p,p'-DDE concentrations were associated with a reduction of BMI z-score at 6 months of age [ß = -0.07, 95% CI: -0.12, -0.01]. Cord serum ß-HCH was also positively associated with the risk of overweight at 12 months of age [RR = 1.16, 95% CI (1.02, 1.33), for the medium vs the lowest tertile]. Among girls, the effects of ß-HCH on BMI z-score and overweight status were stronger than boys at 12 and 24 months of age. No statistically significant relationships of other OCPs with infant growth were observed. CONCLUSIONS: Prenatal exposure to ß-HCH was associated with increased BMI z-score and higher risk of overweight status in infants especially at 12 and 24 months of age, which seemed to be stronger in girls.

15.
Biol Sex Differ ; 12(1): 18, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33516267

RESUMO

BACKGROUND: The mortality of nasopharyngeal carcinoma (NPC) is usually lower in females than in males, but the underlying mechanism remains largely unknown. We sought to describe the age-dependent patterns of gender disparities in NPC survival and explore the extent to which the confounder or mediation effects could explain these differences. METHODS: A total of 11,980 patients with NPC were reviewed. The effect of gender on cancer-specific survival (CSS) was assessed using Cox regression analyses. Two propensity score methods were conducted to control the confounding bias between genders. Restricted cubic spline regression was used to model the association of gender and age with mortality flexibly. Multiple mediation analysis was applied to estimate the direct or indirect effect of gender on CSS. RESULTS: Overall, 7026 males and 2320 females were analyzed. The crude CSS was significantly higher for females than males (10-year CSS 78.4% vs 70.3%; P < 0.001). Similar results were observed after adjusting for confounding bias. Gender disparities in NPC-specific mortality were age-dependent, where they would increase with age until peaking at age 55-60 years and decline rapidly afterward. Subgroup analyses revealed that females' survival advantage was observed in the 18-45 age group and was more prominent in the 46-55 age group, but vanished in the > 55 age group. Either confounder or mediation effects only accounted for approximately 20% of the gender differences. CONCLUSIONS: Gender disparities in cancer-specific mortality for patients with NPC were age-dependent. The differences mostly cannot be explained by confounder or mediation effects.

16.
J Hazard Mater ; 411: 125102, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33461012

RESUMO

Prenatal vanadium exposure is reported to be associated with restricted fetal growth and adverse birth outcomes. However, trimester-specific vanadium exposure in relation to early-childhood growth still remains unclear. A total of 1873 Chinese mother-infant pairs from whom a complete series of maternal urinary samples were collected over three stages of pregnancy were included from 2014 to 2016. The urinary concentrations of vanadium were analyzed. Children's anthropometric parameters were measured at birth, 6, 12 and 24 months. In boys, each doubling increase in vanadium concentrations at middle pregnancy was inversely associated with weight-for-length [- 9.07% (-17.21%, -0.93%)] and BMI z-score [- 9.66% (-18.05%, -1.28%)] at 24 months. Moreover, vanadium exposure at late pregnancy was negatively associated with weight [- 9.85% (-16.42%, -3.28%)], weight-for-length [- 11.00% (-18.40%, -3.60%)], and BMI z-scores [- 11.05% (-18.67%, -3.42%)] at 24 months in boys. However, the negative associations were not observed in girls, and we found evidence for sex difference (FDR p for interaction=0.01, 0.01 and 0.03 for weight, weight-for-length and BMI z-scores, respectively). Prenatal vanadium exposure may have an adverse effect on early-childhood growth, and the middle and late pregnancy could be windows of vulnerability for the adverse effects of vanadium exposure on growth development.

17.
Commun Biol ; 4(1): 105, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33483627

RESUMO

Coconut (Cocos nucifera) is the emblematic palm of tropical coastal areas all around the globe. It provides vital resources to millions of farmers. In an effort to better understand its evolutionary history and to develop genomic tools for its improvement, a sequence draft was recently released. Here, we present a dense linkage map (8402 SNPs) aiming to assemble the large genome of coconut (2.42 Gbp, 2n = 32) into 16 pseudomolecules. As a result, 47% of the sequences (representing 77% of the genes) were assigned to 16 linkage groups and ordered. We observed segregation distortion in chromosome Cn15, which is a signature of strong selection among pollen grains, favouring the maternal allele. Comparing our results with the genome of the oil palm Elaeis guineensis allowed us to identify major events in the evolutionary history of palms. We find that coconut underwent a massive transposable element invasion in the last million years, which could be related to the fluctuations of sea level during the glaciations at Pleistocene that would have triggered a population bottleneck. Finally, to better understand the facultative halophyte trait of coconut, we conducted an RNA-seq experiment on leaves to identify key players of signaling pathways involved in salt stress response. Altogether, our findings represent a valuable resource for the coconut breeding community.

19.
Biotechnol Lett ; 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33496921

RESUMO

OBJECTIVES: Recent studies have revealed that circular RNA (circRNA) plays a pivotal role in cancer development. The study aimed to investigate the role of circ_0081146 in gastric cancer (GC). RESULTS: Circ_0081146 was upregulated in GC tissues and cells. Patients with high expression of circ_0081146 had a significantly reduced 5-year overall survival rate. Circ_0081146 knockdown restrained the growth, migration and invasion of GC cells in vitro as well as tumorigenesis in vivo. Circ_0081146 targeted miR-144 and HMGB1 was targeted by miR-144. Circ_0081146 was negatively correlated with miR-144 expression, while positively correlated with HMGB1 expression in GC tissues. Moreover, the inhibitory effect of circ_0081146 knockdown on the progression of GC cells were reversed by silencing miR-144 or HMGB1 overexpression. Mechanically, circ_0081146 increased HMGB1 expression by targeting miR-144. CONCLUSION: Circ_0081146 functions as an oncogene in GC to promote cell growth, migration and invasion via modulating the miR-144/HMGB1 axis.

20.
Br J Haematol ; 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33481245

RESUMO

Clonal haematopoiesis of indeterminant potential (CHIP) increases in frequency with age. The effect of CHIP on the mobilization of autologous CD34+ peripheral blood stem cells (PBSC) has not been reported. This study uses a DNA-based targeted candidate gene approach to identify the presence of somatic mutations in ASXL1, DNMT3A, JAK2, SF3B1, TET2 and TP53 in CD34+ haematopoietic progenitor cell-apheresis products of 96 patients who undergo PBSC mobilization for autologous stem cell transplantation (ASCT). Variants were identified in a significantly greater proportion of patients who experience poor CD34+ PBSC mobilization. A DNA-based targeted candidate gene array is able to predict poor CD34+ PBSC mobilization and may be deployed pre-emptively to minimize mobilization and graft failures.

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