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1.
NMR Biomed ; : e4260, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040226

RESUMO

This study evaluates the resolution-dependent influences of compressed sensing (CS) in MRI quantification of T2 mapping in articular cartilage with osteoarthritis (OA). T2-weighed 2D experiments of healthy and OA cartilage were fully sampled in k-space with five echo times at both 17.6 µm and 195.3 µm in-plane resolutions; termed as microscopic MRI (µMRI) and macroscopic MRI (mMRI) respectively. These fully sampled k-space data were under-sampled at various 2D CS accelerating factors (AF = 4-32). The under-sampled data were reconstructed individually into 2D images using nonlinear reconstruction, which were used to calculate the T2 maps. The bulk and zonal variations of T2 values in cartilage were evaluated at different AFs. The study finds that the T2 images at AFs up to 8 preserved major visual information and produced negligible artifacts for µMRI. The T2 values remained accurate for different sub-tissue zones at various AFs. The absolute difference between the CS (AF up to 32) and the Ground Truth (i.e., using 100% of the k-space data) of the mean T2 values through the whole tissue depth was higher in mMRI versus µMRI. For mMRI (where the resolution mimics the clinical MRI of human cartilage), the quantitative T2 mapping at AFs up to 4 showed negligible variations. This study demonstrates that both clinical MRI and µMRI can benefit from the use of CS in image acquisition, and µMRI benefits more from the use of CS by acquiring much less data, without losing significant accuracy in the quantification of T2 maps in osteoarthritic cartilage.

2.
J Cell Physiol ; 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32026462

RESUMO

Increasing evidence confirms that exosome-mediated transfer of microRNAs can influence cancer progression including tumor cell invasion, cell proliferation, and drug resistance via cell-cell communication. However, the potential role of exosomal-miR-1260b in lung adenocarcinoma (LAC) remains poorly understood. Thus, this study focused on investigating the function of exosomal-miR-1260b on cell invasion. Exosomal-miR-1260b was found to be higher in plasma of patients with LAC than that of healthy persons via quantitative real-time polymerase chain reaction assay. The sensitivity and specificity of exosomal-miR-1260b (cutoff point: 2.027) were 72% and 86%, and area under the curve of 0.845 (95% CI = 0.772-0.922). Elevated expression of miR-1260b in LAC tissues was positively correlated with exosomal-miR-1260b in plasma (r = .642, p < .05). Furthermore, ceramide biosynthesis regulated exosomal-miR-1260b secretion. Exosome-mediated transfer of miR-1260b promoted A549 cell invasion and was still functional inside A549 cells. Moreover, exosomal-miR-1260b regulated Wnt/ß-catenin signaling pathway by inhibiting sFRP1 and Smad4. This study identified a new regulation mechanism involving in cell invasion by exosome-mediated tumor-cell-to-tumor-cell communication. Targeting exosome-microRNAs may provide new insights into the diagnosis and treatment of LAC.

3.
Nanoscale ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32026906

RESUMO

Bismuth (Bi) is a promising anode material for potassium-ion batteries due to its high energy density. However, the large volume change limits its applications. Herein, N-doped carbon coated Bi nanorods with a hollow structure are fabricated and they exhibit excellent long-term cycling performance (88% capacity retention over 1000 cycles) and high-rate ability (297 mA h g-1 at 20C, 94% capacity of that at 1C). Furthermore, the mechanism was expounded by in situ XRD, indicating a multi-phase reaction for the initial discharge process and three two-phase reactions for the subsequent charge/discharge processes.

4.
J Med Virol ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31916268

RESUMO

Human papillomavirus (HPV) infection was associated with some carcinomas, especially malignant tumors in upper digestive tract, upper respiratory tract, and genitourinary system. The mechanism of the viral transformation of normal cells is still not very clear. To investigate the tumorigenesis of epithelial cells, E6/E7-induced malignant transformation model cells were used for expression profiling analysis by performing RNA expression microarray detection. Bioinformatics analysis was applied to investigate the cellular process changes along with the E6/E7 expression in SHEE cells. The differentially expressed genes were further grouped and uploaded for Search Tool for the Retrieval of Interacting Genes analysis. The protein-protein interaction results were visualized. The hub genes and their first-neighbors genes were selected, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The obtained results demonstrated that tumor-related biological processes began to emerge during the carcinogenesis process from 48th passage to 76th passage of SHEE cells after E6/E7 expression. Ten hub genes were identified and analyzed during the E6/E7-induced tumorigenesis. This study explores the gene expression network in the progressive transformation of immortalized esophageal epithelial cells induced by E6/E7 expression. Understanding the biological processes and hub genes that first appear during the transformation will provide some clues to the mechanism of E6/E7-induced carcinogenesis of esophageal epithelial cells.

5.
Environ Sci Technol ; 54(4): 2539-2547, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-31985212

RESUMO

Development of low-cost, high-efficiency, and environmentally benign adsorbents for mercury removal is of significant importance for environmental remediation. Herein, we report a novel porous puffed rice carbon (PRC) with co-implanted metal iron and sulfur, forming a high-quality PRC/Fe@S composite as a high-efficiency adsorbent for mercury removal from aqueous solution. The in situ-formed Fe nanoparticles in PRC are strongly coupled with sulfur via a supercritical CO2 fluid approach and dispersed homogeneously in the cross-linked hierarchical porous architecture. The pore formation mechanism of Fe on PRC is also proposed. The optimized PRC/Fe@S composite offers superior selective affinity, high removal efficiency, and ultrahigh adsorption capacity of up to 738.0 mg g-1. It is demonstrated that the hierarchical porous carbon in the PRC/Fe@S composite not only acts as a framework to stabilize and disperse Fe nanoparticles but also provides abundant pores and voids for absorbing Hg(II) from aqueous solution. More importantly, the absorbed Hg(II) can be reduced to Hg(0) by Fe and further chemically immobilized by sulfur. The enhanced coupled effect is discussed and proposed. Therefore, an innovative adsorption mechanism of adsorption-reduction-immobilization is proposed, which offers a new prospect in developing high-efficiency carbon-based adsorbents in environmental remediation.

6.
Dig Dis Sci ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31993894

RESUMO

BACKGROUND AND AIMS: The objective of this study was to construct and authenticate nomograms to project overall survival (OS) and cancer-specific survival (CSS) in primary gastrointestinal non-Hodgkin lymphomas (PGINHL). METHODS: Suitable patients were chosen from the Surveillance, Epidemiology and End Results database and Wannan Medical College Yijishan Hospital. The Cox regression model was used to acquire independent predictive factors to develop nomograms for projecting OS and CSS. The performance of the nomograms was validated using the Harrell's concordance index (C-index), calibration curves, and decision curve analysis (DCA) and was compared with that of the AJCC 7th staging system. Survival curves were obtained using the Kaplan-Meier method, while the log-rank test was used to compare the difference among the groups. RESULTS: The C-index of the nomograms for OS and CSS was 0.735 (95% CI = 0.719-0.751) and 0.761 (95% CI = 0.739-0.783), respectively, signifying substantial predictive accuracy. These outcomes were reproducible when the nomograms were used for the internal and external validation cohorts. Moreover, assessments of the C-index, AUC, and DCA between the nomogram results and the AJCC 7th staging system showed that the former was better for evaluation and was more clinically useful. CONCLUSIONS: We constructed the nomogram which could predict 1-, 3-, and 5-year OS and CSS of patients with PGINHL. Our nomogram showed good performance, suggesting that it can be used as an efficacious instrument for predictive assessment of patients with PGINHL.

7.
FASEB J ; 2020 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-31930569

RESUMO

Recent evidence indicates that elevated placental adenosine signaling contributes to preeclampsia (PE). However, the molecular basis for the chronically enhanced placental adenosine signaling in PE remains unclear. Here, we report that hypoxia-inducible factor-1α (HIF-1α) is crucial for the enhancement of placental adenosine signaling. Utilizing a pharmacologic approach to reduce placental adenosine levels, we found that enhanced adenosine underlies increased placental HIF-1α in an angiotensin receptor type 1 receptor agonistic autoantibody (AT1 -AA)-induced mouse model of PE. Knockdown of placental HIF-1α in vivo suppressed the accumulation of adenosine and increased ecto-5'-nucleotidase (CD73) and adenosine A2B receptor (ADORA2B) in the placentas of PE mouse models induced by AT1 -AA or LIGHT, a TNF superfamily cytokine (TNFSF14). Human in vitro studies using placental villous explants demonstrated that increased HIF-1α resulting from ADORA2B activation facilitates the induction of CD73, ADORA2B, and FLT-1 expression. Overall, we demonstrated that (a) elevated placental HIF-1α by AT1 -AA or LIGHT upregulates CD73 and ADORA2B expression and (b) enhanced adenosine signaling through upregulated ADORA2B induces placental HIF-1α expression, which creates a positive feedback loop that promotes FLT-1 expression leading to disease development. Our results suggest that adenosine-based therapy targeting the malicious cycle of placental adenosine signaling may elicit therapeutic effects on PE.

8.
ChemSusChem ; 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31943838

RESUMO

The activity and selectivity of simple photocatalysts for CO2 reduction remain limited by the insufficient photophysics of the catalysts, as well as the low solubility and slow mass transport of gas molecules in/through aqueous solution. In this study, these limitations are overcome by constructing a triphasic photocatalytic system, in which polymeric carbon nitride (CN) is immobilized onto a hydrophobic substrate, and the photocatalytic reduction reaction occurs at a gas-liquid-solid (CO2 -water-catalyst) triple interface. CN anchored onto the surface of a hydrophobic substrate exhibits an approximately 7.2-fold enhancement in total CO2 conversion, with a rate of 415.50 µmol m-2 h-1 under simulated solar light irradiation. This value corresponds to an overall photosynthetic efficiency for full water-CO2 conversion of 0.33 %, which is very close to biological systems. A remarkable enhancement of direct C2 hydrocarbon production and a high CO2 conversion selectivity of 97.7 % are observed. Going from water oxidation to phosphate oxidation, the quantum yield is increased to 1.28 %.

9.
Aging (Albany NY) ; 12(1): 309-317, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31899685

RESUMO

OBJECTIVE: This study aimed to determine whether changes in sleep duration are associated with a higher risk of mild cognitive impairment (MCI) in older adults. RESULTS: By the 3-year follow-up, 592 participants developed MCI. Compared with the individuals who had an unchanged sleep duration, the odds ratio (95% confidence interval) for MCI was 1.44 (1.08-1.91) for those whose sleep duration increased by ≥2 h after multivariate adjustments. Moreover, changing from a long to moderate, but not short, sleep duration was negatively associated with the incidence of MCI (odds ratio: 0.65; 95% confidence interval, 0.45-0.93). CONCLUSIONS: These findings suggest that increased sleep duration is associated with a higher risk of MCI in the elderly. Furthermore, a moderate duration of sleep (6-9 h) could serve as a possible strategy for prevention of MCI. METHODS: This longitudinal study was conducted with a nationally representative sample of 5419 older Chinese adults (≥65 years) from the 2008 and 2011 waves of the Chinese Longitudinal Healthy Longevity Survey. Sleep duration was assessed by a self-administered questionnaire. MCI was defined according to the Mini-Mental State Examination. An adjusted logistic regression model was used to explore the associations between changes in sleep duration and MCI.

10.
Cancer Epidemiol Biomarkers Prev ; 29(1): 103-111, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31575556

RESUMO

BACKGROUND: Heterocyclic aromatic amines (HAA) are a group of hazardous substances produced during combustion of tobacco or high-temperature cooking of meats. 2-Amino-9H-pyrido[2,3-b]indole (AαC) is a major carcinogenic HAA in tobacco smoke. METHODS: Urinary AαC, used as a marker of AαC exposure, was analyzed on spot urine samples from adult participants of the 2013-2014 cycle of the National Health and Nutrition Examination Survey (N = 1,792). AαC was measured using isotope-dilution liquid chromatography-tandem mass spectrometry. Exclusive combusted tobacco smokers were differentiated from nonusers of tobacco products through both self-report and serum cotinine data. RESULTS: Among exclusive smokers, sample-weighted median urinary AαC was 40 times higher than nonusers. Sample-weighted regression models showed that urinary AαC increased significantly with serum cotinine among both exclusive tobacco users and nonusers with secondhand smoke exposure. Among nonusers, eating beef cooked at high temperature was associated with a significant increase in urinary AαC, whereas consuming vegetables was associated with decreased AαC. In addition, smoking one-half pack of cigarettes per day was associated with a significant increase of 23.6 pg AαC/mL calculated at geometric mean of AαC, controlling for potential confounders. In comparison, increase in AαC attributable to consuming the 99th percentile of beef cooked at high temperature was 0.99 pg AαC/mL. CONCLUSIONS: Both exclusive smokers and nonusers of tobacco in the general U.S. population are exposed to AαC from tobacco smoke, with additional, lesser contributions from certain dietary components. IMPACT: AαC is an important biomarker that is associated with tobacco smoke exposure.

11.
J Hum Hypertens ; 34(1): 59-67, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31551568

RESUMO

Previous studies have shown that allicin can lower blood pressure (BP) by reducing oxidative stress and inflammation. However, the association between habitual raw garlic intake (as allicin source) and prehypertension are unclear. The aim of this study was to investigate how raw garlic consumption is associated with prehypertension in an adult population. A cross-sectional study was conducted with 22,812 adults (mean [standard deviation] age: 39.4 [10.7] years; males, 47.7%) in Tianjin, China. Raw garlic consumption was assessed using a validated food frequency questionnaire. BP was measured at least twice by trained nurses using an automatic device. Prehypertension was defined as systolic BP of 120-139 mmHg and/or diastolic BP of 80-89 mmHg without taking antihypertensive medication. Multiple logistic regression models were used to assess the association between raw garlic consumption and prehypertension. The prevalence of prehypertension was 49.9%. After fully adjusting for potential confounders, the ORs (95% confidence intervals) of having prehypertension by increasing frequency of raw garlic consumption were 1.00 (reference) for ≤3 times/week, 0.96 (0.87, 1.06) for 4 times/week to 1 time/day, and 0.69 (0.52, 0.90) for ≥2 times/day (p for trend = 0.06). In contrast, no associations were observed between other kinds of allium vegetables consumption and prehypertension. In conclusion, our results suggested that a more frequent consumption of raw garlic was inversely associated with prehypertension. This is the first large-scale study on the association between raw garlic consumption and prehypertension in the general population.

12.
Huan Jing Ke Xue ; 41(1): 133-138, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854913

RESUMO

In typical cities of East China, more than 900 non-road vehicles were tested for exhaust smoke. Based on the investigation of the properties of these non-road vehicles, exhaust smoke intensities for different kinds of non-road vehicles are recommended. We also quantitatively study the differences in smoke intensity among vehicle age, vehicle power, test conditions, and fuels. The results showed that smoke intensity of non-road vehicles was (1.02±0.57) m-1 and that Ringelmann smoke was 2.10±0.19. In comparison to Chinese national standard (GB 36886), approximately 12%-25% of tested non-road vehicles' smoke intensity exceeded the standard limit. The smoke intensity of 80% of tested non-road vehicles was higher during start-up than under free acceleration. In comparison to ordinary diesel, the smoke intensities of tested non-road vehicles that used automotive diesel were lower. The instantaneous increase in fuel injection during start-up, as well as poor fuel quality, can directly affect the exhaust smoke of non-road vehicles.

13.
J Magn Reson ; 311: 106669, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31881481

RESUMO

We consider the spin lattice relaxation in bulk liquid and liquid entrapped in a nanocavity. The kinetic equation which describes the spin lattice relaxation is obtained by using the theory of the nonequilibrium state operator. A solution of the kinetic equation gives the quadrature expression for the relaxation time, T1. The calculated relaxation time agrees well with the experimental data. The spin-lattice relaxation time is calculated for nanocavities with a characteristic size much less than 700 nm, with the assumption that the spin-lattice relaxation mechanism is determined by nanocavity fluctuations. The resulting expression shows an explicit dependence of the relaxation time T1 on the volume, density of nuclear spins, and parameters of the cavity (shape and orientation relatively to the applied field). To compare with the experiment on the detection of the anisotropy of the relaxation time, we average the expression that describes the relaxation time over the orientation of the nanocavities relative to the applied magnetic field. The good agreement with the experimental data for fibril tissues was achieved by adjustment of few fitting parameters - the standard deviation, averaged fiber direction, and weight factors - which characterize the ordering of fibrils.

14.
Clin Case Rep ; 7(11): 2019-2021, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31788244

RESUMO

This case enforces the importance of a patient's medical history in assisting with the diagnosis of nonspecific cutaneous lesions. Also, other types of lesions occurring within a scar are reviewed.

15.
J Orthop Res ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31799697

RESUMO

This study aimed to establish the baseline characteristics in humeral and femoral cartilage in rabbit, using quantitative magnetic resonance imaging (MRI) relaxation times (T2, T1ρ, and T1) at 9.75 and 70-82 µm pixel resolutions, and quantitative polarized light microscopy (PLM) measures (retardation, angle) at 1.0 and 4.0 µm pixel resolutions. Five intact (i.e., unopened) shoulder joints (the scapula and humeral heads) and three femoral heads of the hip joints from five healthy rabbits were imaged in MRI at 70-82 µm resolution. Thirteen cartilage-bone specimens were harvested from these joints and imaged in µMRI at 9.75 µm resolution. Subsequently, quantitative PLM study of these specimens enabled the examination of the fibril orientation and organization in both intact joints and individual specimens. Quantitative MRI relaxation data and PLM fibril structural data show distinct features in tissue properties at different depths of cartilage, different in individual histological zones. The thicknesses of the histological zones in µMRI and PLM were successfully obtained. This is the first correlated and quantitative MRI and PLM study of rabbit cartilage at sub-10 µm resolutions, which benefits future investigation of osteoarthritis using the rabbit model. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

16.
Nutrition ; 71: 110562, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31809956

RESUMO

OBJECTIVE: Previous studies have demonstrated that nutritional status is associated with non-alcoholic fatty liver disease (NAFLD). Meanwhile, subclinical inflammation is associated with the prevalence of NAFLD. To our knowledge, however, no study has investigated the intermediary effect of inflammation on the association between dietary pattern and NAFLD. Thus, the aim of this case-control study was to explore the intermediary effect of inflammation on the association between dietary patterns and NAFLD. METHODS: Using the propensity score matching method, we generated 2043 cases and 2043 controls. Dietary intake was assessed using a valid self-administered food frequency questionnaire. Major dietary patterns in the population were derived by factor analysis. Reduced rank regression with leukocyte count as a response variable was used to derive an inflammatory pattern. NAFLD was diagnosed by liver ultrasonography and drinking history. The association between dietary patterns and NAFLD was tested using multiple conditional logistic regression analysis. RESULTS: Three major dietary patterns were derived by factor analysis: sweet pattern, animal foods pattern, and traditional pattern. Compared with participants in the lowest quartile of the animal foods pattern, the odds ratio (OR; 95% confidence interval [CI]) of NAFLD in the highest quartile was 1.30 (95% CI, 1.09-1.55). After adjustment of inflammation status, the OR was weaker (OR, 1.23; 95% CI, 1.03-1.48), albeit significant. Compared with the participants in the lowest quartile of inflammatory pattern, the OR of NAFLD in the highest quartile was 1.52 (95% CI, 1.28-1.81). CONCLUSION: The present data suggested that inflammation may mediate the association between dietary patterns and NAFLD.

17.
Int J Neural Syst ; : 1950029, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31847633

RESUMO

Status epilepticus (SE) is a common, life-threatening neurological disorder that may lead to permanent brain damage. In rodent models, SE is an acute phase of seizures that could be reproduced by injecting with pilocarpine and then induce chronic temporal lobe epilepsy (TLE) seizures. However, how SE disrupts brain activity, especially communications among brain regions, is still unclear. In this study, we aimed to identify the characteristic abnormalities of network connections among the frontal cortex, hippocampus and thalamus during the SE episodes in a pilocarpine model with functional and effective connectivity measurements. We showed that the coherence connectivity among these regions increased significantly during the SE episodes in almost all frequency bands (except the alpha band) and that the frequency band with enhanced connections was specific to different stages of SE episodes. Moreover, with the effective analysis, we revealed a closed neural circuit of bidirectional effective interactions between the frontal regions and the hippocampus and thalamus in both ictal and post-ictal stages, implying aberrant enhancement of communication across these brain regions during the SE episodes. Furthermore, an effective connection from the hippocampus to the thalamus was detected in the delta band during the pre-ictal stage, which shifted in an inverse direction during the ictal stage in the theta band and in the theta, alpha, beta and low-gamma bands during the post-ictal stage. This specificity of the effective connection between the hippocampus and thalamus illustrated that the hippocampal structure is critical for the initiation of SE discharges, while the thalamus is important for the propagation of SE discharges. Overall, our results demonstrated enhanced interaction among the frontal cortex, hippocampus and thalamus during the SE episodes and suggested the modes of information flow across these structures for the initiation and propagation of SE discharges. These findings may reveal an underlying mechanism of aberrant network communication during pilocarpine-induced SE discharges and deepen our knowledge of TLE seizures.

18.
Clin Exp Hypertens ; : 1-8, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31760826

RESUMO

Background: Honey has been shown to have multiple positive effects on human health. However, data on the associations of honey consumption with prehypertension are scarce. The aim of this study was to investigate whether honey consumption is associated with prehypertension in a Chinese population. Method: A cross-sectional study was conducted in 4,561 individuals aged ≥40 years. A validated 100-item semi-quantitative food frequency questionnaire was used to assess honey consumption. Blood pressure was measured at least twice by trained nurses. Multiple logistic regression models were used to explore the associations between honey consumption and prehypertension. Results: After adjustment for potential confounders, the odds ratios and 95% confidence intervals of prehypertension across increasing frequency of honey consumption were 1.00 (reference) for almost never, 1.17 (0.96, 1.41) for ≤6 times/week, and 1.25 (0.86, 1.84) for ≥7 times/week in men (P for trend = 0.09); 1.00 (reference) for almost never, 0.76 (0.62, 0.92) for ≤6 times/week, and 0.84 (0.63, 1.12) for ≥7 times/week in women (P for trend = 0.055), respectively. Conclusion: Our results showed that light-to-moderate intake of honey was associated with lower prevalence of prehypertension in women, but not men. Future studies are required to confirm these associations.

19.
Nano Lett ; 19(12): 8724-8731, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31682449

RESUMO

Two-dimensional (2D) superlattices composed of chemically heterogeneous transition-metal dichalcogenides (TMDs) have been proposed as key components in next-generation optoelectronic devices. For potential applications, coherent, defect-free compositional interfaces are usually required. In this paper, a combination of scaling theory and numerical analysis is employed to investigate strain relaxation mechanisms in misfitting, chemically heterogeneous TMDs. We demonstrate that, in free-standing superlattices, wrinkling of the monolayer is asymptotically preferred over misfit dislocation formation in both binary and ternary superlattices. For substrate-supported monolayers, however, misfit dislocation formation is thermodynamically favored above a critical superlattice width, implying the presence of an upper limit to the thermodynamic stability of coherent, misfitting 2D superlattices. Finally, it is shown numerically that the critical superlattice width is only weakly dependent on the misfit.

20.
J Pain Res ; 12: 2851-2863, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695479

RESUMO

Background: Neuropathic pain with complications greatly affects patients worldwide. High mobility group box 1 (HMGB1) has been shown to contribute to the pathogenesis of neuropathic pain; thus, suppression of HMGB1 may provide a novel therapeutic option for neuropathic pain. Electroacupuncture (EA) has been indicated to be effective in attenuating neuropathic pain, but the underlying mechanism remains to be fully clarified. We aim to explore whether 2Hz EA stimulation regulates the spinal HMGB1/NF-κB signaling in neuropathic pain induced by spared nerve injury (SNI). Materials and methods: Paw withdrawal threshold and CatWalk gait analysis were used to assess the effect of 2Hz EA on pain-related behaviors in SNI rats. Administration of 2Hz EA to SNI rats once every other day lasting for 21 days. Expression of spinal protein molecules were detected using Western blot and immunofluorescence staining. Results: It was found that SNI significantly induced mechanical hypersensitivity and decrease of gait parameters, and subsequently increased the levels of HMGB1, TLR4, MyD88, and NF-κB p65 protein expression. 2Hz EA stimulation led to remarkable attenuation of mechanical hypersensitivity, upregulation of spinal HMGB1, TLR4, MyD88, and NF-κB p65 protein expressions induced by SNI, and significant improvement in gait parameters. Furthermore, immunofluorescence staining also confirmed that 2Hz EA obviously suppressed the co-expression of microglia activation marker CD11b and TLR4 or MyD88, as well as the activation of NF-κB p65 in SNI rats. Conclusion: This study suggested that blockade of HMGB1/NF-κB signaling in the spinal cord may be a promising therapeutic approach for 2Hz EA management of SNI-induced neuropathic pain.

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