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Int J Biol Macromol ; 185: 1022-1035, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34197859


Biochemical modification can endow the surface of implants with superior biological activity. Herein, silk fibroin (SF) protein and its anionic derivative peptides (Cs) were covalently immobilized onto a titanium implant surface via a polydopamine layer. The successful conjugation of SF and Cs was revealed by X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and contact angle measurements. The addition of Cs prevented the conformational transition of silk fibroin to silk II. The deposition of apatite on its surface was significantly accelerated, and the bioactive composite coating was observed to enhance protein adsorption and cell proliferation. More importantly, it also promoted the osteogenic differentiation of bone marrow stem cells (BMSCs) for the quantitative and qualitative detection of alkaline phosphatase (ALP) and alizarin red (ARS). Overall, the stable performance and enhanced osteogenic property of the composite coating promote an extensive application for clinical titanium-based implants.

Fibroínas/farmacologia , Indóis/química , Células-Tronco Mesenquimais/citologia , Osteogênese/efeitos dos fármacos , Polímeros/química , Titânio/química , Adsorção , Animais , Apatitas/química , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Fibroínas/química , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Microscopia de Força Atômica , Oxirredução , Peptídeos/química , Peptídeos/farmacologia , Espectroscopia Fotoeletrônica
Biomed Mater ; 16(4)2021 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-34098538


In this study, the silk fibroin/nano-hydroxyapatite/hyaluronic acid (SF/nHAp/HA) composite scaffolds with different HA contents were developed by blending, cross-linking and freeze-drying, and their physicochemical properties and cell biocompatibilityin vitrowere subsequently studied. It was observed that the molecular conformation of the composite scaffolds was mainly composed of silk I and a small amount of theß-sheets structure. On enhancing the HA content, the pore size of the scaffold decreased, while the porosity, water absorption, swelling ratio and mechanical properties were observed to increase. In particular, the SF/nHAp/HA scaffold with a 5.0 wt% ratio exhibited the highest water absorption and mechanical properties among the developed materials. In addition, thein vitrocytocompatibility analysis showed that the bone marrow mesenchymal stem cells exhibited excellent cell proliferation and osteogenic differentiation ability on the SF/nHAp/5.0 wt%HA scaffolds, as compared with the other scaffolds. It can be concluded that the developed composite scaffolds represent a promising class of materials for the bone tissue repair and regeneration.

IEEE J Transl Eng Health Med ; 9: 2600109, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33598368


Objective: To remove blood from an incision and find the incision spot is a key task during surgery, or else over discharge of blood will endanger a patient's life. However, the repetitive manual blood removal involves plenty of workload contributing fatigue of surgeons. Thus, it is valuable to design a robotic system which can automatically remove blood on the incision surface. Methods: In this paper, we design a robotic system to fulfill the surgical task of the blood removal. The system consists of a pair of dual cameras, a 6-DoF robotic arm, an aspirator whose handle is fixed to a robotic arm, and a pump connected to the aspirator. Further, a path-planning algorithm is designed to generate a path, which the aspirator tip should follow to remove blood. Results: In a group of simulating bleeding experiments on ex vivo porcine tissue, the contour of the blood region is detected, and the reconstructed spatial coordinates of the detected blood contour is obtained afterward. The BRR robot cleans thoroughly the blood running out the incision. Conclusions: This study contributes the first result on designing an autonomous blood removal medical robot. The skill of the surgical blood removal operation, which is manually operated by surgeons nowadays, is alternatively grasped by the proposed BRR medical robot.

Chemosphere ; 241: 125010, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31605993


In this study, electrochemical oxidation of Acid Orange 7 (AO 7) azo dye has been investigated using a Fe-doped PbO2 electrode. The degradation of AO 7 followed pseudo-first-order reaction kinetics. The removals of AO 7, chemical oxygen demand (COD) and total organic carbon (TOC) were 87.15%, 49.88% and 44.94% after 60 min of electrolysis at the optimal conditions (Na2SO4 concentration 0.1 M, initial pH 5, initial AO 7 concentration 100 mg L-1 and applied current density 20 mA cm-2), respectively. And the corresponding degradation rate constant was 0.035 min-1. The intermediates formed during electrochemical process were identified, and a possible degradation pathway was proposed, which was initiated by the oxidation of azo bond (-NN-), hydroxylation and substitution reaction of -NH2 and -SO3H under the attack of OH, and ended with the formation of mineralization products such as NH4+, NO3-, SO42-, CO2 and H2O. The toxicity of treated AO 7 solution towards Vibrio fischeri increased slightly at first and then rapidly reduced to non-toxicity with prolonging time. The results indicate that electrochemical oxidation of AO 7 using Fe-doped PbO2 electrode is a promising way.

Compostos Azo/química , Benzenossulfonatos/química , Técnicas Eletroquímicas/métodos , Eletrólise/métodos , Chumbo/química , Óxidos/química , Poluentes Químicos da Água/química , Análise da Demanda Biológica de Oxigênio , Eletrodos , Eletrólise/normas , Cinética , Oxirredução , Poluentes Químicos da Água/análise
Chemosphere ; 205: 215-222, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29698833


In this work, the electrochemical degradation of antibiotic levofloxacin (LFX) has been studied using a novel rare earth La, Y co-doped PbO2 electrode. The effect of applied current density, pH value and initial LFX concentration on the degradation performance were systematically evaluated. The results demonstrated that electrochemical oxidation of LFX over the La-Y-PbO2 electrode was highly effective and the reaction followed an apparent first-order kinetic model. Considering the degradation efficiency and energy efficiency, the relative optimal conditions are identified as current density 30 mA cm-2, pH 3 and initial LFX concentration 800 mg L-1. According to the identified products, a reaction mechanism has been proposed and the products were further oxidized to CO2, H2O, NH4+, NO3- and F-. A total of four aromatic intermediate products of LFX degradation were identified and the different structural changes to the LFX molecule included pepiperazinyl hydroxylation, decarboxylation and defluorination.

Antibacterianos/química , Técnicas Eletroquímicas/métodos , Eletrodos , Chumbo/química , Levofloxacino/química , Óxidos/química , Poluentes Químicos da Água/química , Cinética , Oxirredução