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1.
J Phys Chem A ; 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32436382

RESUMO

In order to provide feasible methods for the extraction of valuable metals from spent batteries or low-grade primary ores, the extraction behavior of some representative acidic phosphorus-containing compounds (APCC) as extractants is evaluated from the perspective of experimental and theoretical investigations in this work. Aqueous solutions containing five metal ions, Ca(II), Co(II), Mg(II), Mn(II), and Ni(II), were made to simulate leaching liquids, and the extraction of these metals were investigated. A simplified calculated model was used to evaluate the interaction between each extractant and metal ions. The calculation results agree well with the experimental tests in trend. This work not only provides potential extractants for the extraction of valuable metals from spent batteries or low-grade primary ores, but also demonstrates the practicability of the simplified calculation model.

2.
J Insect Physiol ; 119: 103951, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31563619

RESUMO

Intermittent food shortages are commonly encountered in the wild. To cope with the threat of starvation, insects initiate a suite of behavioral activities and physiological countermeasures. The cotton bollworm, Helicoverpa armigera, is a major agricultural pest worldwide, but how H. armigera modulates its metabolism under starvation remains ambiguous. In the present study, the respiratory rates (V̇O2; ml g-1 h-1) from the third-larval instar to the pupal stage were first determined. Our results highlighted a transient rise during the larval-larval molt and larval-pupal transition, followed by a sharp decline in the pupal stage and, finally, an upward trend before eclosion. When subjected to food deprivation, the starved larvae experienced a significant decline in the rates of O2 consumed and CO2 produced, as well as in respiratory quotient (RQ) values, indicative of severe metabolic depression during starvation and a shift of metabolic substrates with prolonged starvation. For metabolic substrate analysis, an apparent decline in triglyceride and glycogen contents was observed in the starved larvae, and the hemolymph trehalose content was significantly reduced throughout starvation. In addition, comparative transcriptome analysis showed that 48 h of larval starvation caused substantial transcriptional regulations in several energetically costly processes, wherein the marked up-regulations were detected in the pathways of glycolysis and fatty acid metabolism. Overall, our work makes a comprehensive study on the respiratory rate and energy metabolism in the starved H. armigera larvae, and provides a deep insight into the physiological adaptive strategies to alleviate nutritional stress.

3.
Front Neurol ; 10: 121, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30837940

RESUMO

Objective: To explore the clinical features and correlates of excessive daytime sleepiness (EDS) in a Chinese population of Parkinson's disease (PD) patients. Methods: Patients with clinically established or clinically probable PD were recruited. Clinical and demographic data were collected, and participants were evaluated using standardized assessment protocols. Patients were divided into PD with EDS and PD without EDS groups based on the Epworth sleepiness scale (ESS) scores, with a cutoff score of 10. Clinical manifestations were compared between patients with and without EDS, and correlates of EDS were also studied. In addition, the relationship between EDS and poor nighttime sleep quality was analyzed. Results: A total of 1,221 PD patients were recruited in our study. The mean ESS (min, max) score was 7.6 ± 6.1 (0, 24), and 34.1% of the patients had ESS scores ≥10. No difference was seen in lifestyle (except for alcohol consumption), environmental factors, BMI, levodopa equivalent dose (LED), initial presentation of motor symptoms, motor subtype, and wearing off between patients with and without EDS. The PD with EDS group had a higher proportion of male patients and a higher average patient age. Moreover, the PD with EDS group showed older age at PD onset, lower educational level, and longer disease duration. Patients with EDS had higher scores on the Hoehn-Yahr scale and the Unified Parkinson's Disease Rating Scale (UPDRS) parts I, II, and III score, more severe non-motor symptoms, and poorer quality of sleep and life. Logistic regression analyses demonstrated that EDS was associated with male sex, age, cognitive impairment, PD-related sleep problems, rapid eye movement sleep behavior disorder (RBD), and worse quality of life (QoL). Conclusion: EDS is a general clinical manifestation in PD, and there were significant differences in clinical features between patients with and without EDS. Moreover, our study proved that many factors were associated with EDS, including male sex, age, cognitive impairment, PD-related sleep problems, RBD, and worse QoL. Understanding the clinical characteristics of EDS in PD patients may help identify EDS early, improve QoL, and reduce the occurrence of accidents.

4.
Artif Cells Nanomed Biotechnol ; 46(sup3): S661-S670, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30307317

RESUMO

Hepatocellular carcinoma (HCC) is one of the greatest public health problems worldwide, and chemotherapy remains the major approach for the HCC treatment. Doxorubicin (DOX) is one of the anthracycline antibiotics but its clinical use is limited due to its severe cardiotoxicity. In this study, novel hybrid nanoparticles by self-assembling based on pectin-doxorubicin conjugates (PDC-NPs) were fabricated for HCC treatment. The stabilized structure of the PDC-NPs was characterized by methylene blue absorption, the size, zeta potential and the morphology, which was investigated by Zetasizer nanoparticle analyzer and transmission electron microscope (TEM), of nanoparticles. The PDC-NPs achieved a sustained and prolonged release ability, which was illustrated with in vitro drug release profiles, anti-cell proliferation study, cellular uptake assay and in vivo pharmacokinetics analysis. Biocompatibility of the PDC-NPs was assessed with bovine serum albumin (BSA) adsorption test, hemolysis activity examination and viability evaluation of human umbilical vein endothelial cells. Importantly, in vivo studies of the PDC-NPs, which were performed in the athymic BALB/c nude mice, demonstrated that the PDC-NPs significantly reduced the lethal side effect of DOX. Additionally, the H&E staining and serum biochemistry study further confirmed the excellent biological security of the PDC-NPs.


Assuntos
Carcinoma Hepatocelular , Doxorrubicina , Neoplasias Hepáticas , Nanopartículas , Pectinas , Animais , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacocinética , Preparações de Ação Retardada/farmacologia , Doxorrubicina/química , Doxorrubicina/farmacocinética , Doxorrubicina/farmacologia , Células Hep G2 , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanopartículas/química , Nanopartículas/uso terapêutico , Pectinas/química , Pectinas/farmacocinética , Pectinas/farmacologia , Ensaios Antitumorais Modelo de Xenoenxerto
5.
Clin Transl Sci ; 11(4): 428-434, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29697202

RESUMO

As a proinflammatory cytokine, CD137 (4-1BB, TNFRSF9) is present in membrane-bound and soluble forms. Increased expression of CD137 was recently found in T cells in human atherosclerotic plaques. However, the exact role of CD137 in ischemic stroke is not clear. In this study we analyzed the protein levels of soluble CD137 (sCD137) and the expression of CD137 on CD4+ T cells in the peripheral blood of patients with acute atherothrombotic stroke by using the cytometry beads array (CBA) and flow cytometry. Within 24 hours of onset, the stroke patients showed elevated levels of sCD137 (2.7 pg/ml) and CD137 expression on CD4+ T cells (4.9 ± 3.2%) compared with normal controls (1.1 pg/ml, P < 0.01; 1.3 ± 1.0%, P < 0.01). Alterations in CD137 expression may enhance ischemia-induced inflammatory responses via bidirectional signaling and, consequently, aggravate brain injury in early stages of this disorder.


Assuntos
Aterosclerose/sangue , Linfócitos T CD4-Positivos/metabolismo , Acidente Vascular Cerebral/sangue , Trombose/sangue , Membro 9 da Superfamília de Receptores de Fatores de Necrose Tumoral/sangue , Idoso , Aterosclerose/complicações , Aterosclerose/imunologia , Linfócitos T CD4-Positivos/imunologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/imunologia , Trombose/etiologia , Trombose/imunologia , Membro 9 da Superfamília de Receptores de Fatores de Necrose Tumoral/imunologia , Membro 9 da Superfamília de Receptores de Fatores de Necrose Tumoral/metabolismo
6.
Zhen Ci Yan Jiu ; 43(1): 14-9, 2018 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-29383888

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) on mechanical hyperalgesia threshold (MHTs) and thermal hyperalgesia threshold (THTs) and content of proteinase-activated receptors 2 (PAR 2) in dorsal root ganglia (DRG) in rats with inflammatory pain, so as to explore its peripheral mechanism underlying improvement of inflammatory pain. METHODS: The present study contains two parts. 1) In the first part, 27 male SD rats were randomized into sham hyperalgesic priming (sham-HP) group and real hyperalgesic priming (HP) group (n=5 in the sham-HP group and n=6 in the HP group for the test of MHTs, n=8 in the two groups for the test of THTs). The sham-HP model was established by subcutaneous injection of normal saline into the left plantar part of the hind-paw, and the HP model established by subcutaneous injection of 1% carragenan (the first injection) into the same left hind paw, followed by injection of PGE2 (100 ng/25 µL, the second injection) into the dorsum pedis of the same hind paw 7 days after the first injection. The ipsilateral paw withdrawal latencies (MHTs and THTs) were detected before and 5 h, 3 d and 6 d after the first injection, 0.5, 4 and 24 h after the second injection. 2) In the second part, 64 male SD rats were randomly divided into sham-HP, HP, sham-EA and EA groups (n=16 in each group). The sham-HP and HP models were made in the same way as the first part. Both"Zusanli"(ST 36)and "Kunlun"(BL 60) were punctured with filiform needles in the sham-EA group and also stimulated with EA: 2 Hz/100 Hz, 0.5-1.5 mA (0.5 mA increase per 10 min) for 30 min in the EA group, 1 time/d for 7 d. Both ipsilateral MHTs and THTs were observed at the same time-points of the first part and the PAR 2 protein content in the L 4-L 6 DRGs was assayed by ELISA 24 h after the second injection. RESULTS: 1) In the first part of the study, compared with the sham-HP group, the MHTs at 5 h and 3 d, and THT at 5 h after the first injection, and MHTs, and THTs at 4 and 24 h after the se-cond injection were significantly decreased in the HP group (P<0.01, P<0.05). 2) In the second part of the study, compared with the HP group, the MHTs at 4 and 24 h after the second injection and the THTs at 3 d after the first injection, 4 and 24 h after the second injection were significantly up-regulated in the EA group (P<0.01, P<0.05). The content of PAR 2 in the DRGs (L 4-L 6) was significantly higher in the HP group than in the sham-HP group (P<0.05), but considerably lower in the EA group than in the HP group (P<0.05). CONCLUSION: EA can suppress hyperalgesia priming in inflammatory pain rats which may be related to its effect in down-regulating PAR 2 level in the lumbar DRGs.


Assuntos
Eletroacupuntura , Hiperalgesia , Animais , Gânglios Espinais , Masculino , Dor , Ratos , Ratos Sprague-Dawley , Receptor PAR-2
7.
Chin Med J (Engl) ; 130(19): 2296-2301, 2017 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-28937034

RESUMO

BACKGROUND: As a traditional Chinese medicine, Cordyceps sinensis (CS) possesses a variety of immunoregulatory properties. This study aimed to explore the therapeutic potential of CS in a mice model of multiple sclerosis (MS)-experimental autoimmune encephalomyelitis (EAE). METHODS: Female C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein35-55to induce EAE, followed by an instant intragastric feeding with a low dosage of CS (low-CS group, n = 5), high dosage of CS (high-CS group, n = 5), or the same volume of normal saline (control group, n = 5). All the mice were observed for clinical assessment. Over the 30 days of CS treatment, flow cytometry was used to detect the frequency of helper T-cell (Th) subsets, Th1 and Th17, and CD4+ CD25+ regulatory T cells in the spleen and lymph nodes. Meanwhile, pathological changes in brain were determined using both hematoxylin-eosin and luxol fast blue staining. Data were analyzed using the one-way analysis of variance (ANOVA). RESULTS: Over the 15 and 30 days of CS treatment, the clinical assessment for EAE demonstrated that both high-CS group (2.51 ± 0.31 and 2.26 ± 0.39 scores, respectively) and low-CS group (2.99 ± 0.40 and 2.69 ± 0.46, respectively) had lower disease severity scores than those of control group (3.57 ± 0.53 and 3.29 ± 0.53, all P < 0.01, respectively). Meanwhile, after 15 and 30 days, the high-CS group (19.18 ± 1.34 g and 20.41 ± 1.56 g, respectively) and low-CS group (18.07 ± 1.18 g and 19.48 ± 1.69 g, respectively) had a lower body weight, as compared with control group (16.85 ± 1.15 g and 18.22 ± 1.63 g, all P < 0.01, respectively). At 30 days post-CS treatment, there was a lower Th1 frequency in the lymph nodes (2.85 ± 1.54% and 2.77 ± 1.07% vs. 5.35 ± 1.34%, respectively; P < 0.05) and spleens (3.96 ± 1.09% and 3.09 ± 0.84% vs. 5.07 ± 1.50%, respectively; P < 0.05) and less inflammatory infiltration and demyelination in the brain of CS-treated mice than that of control group. CONCLUSIONS: Our preliminary study demonstrated that CS efficiently alleviated EAE severity and EAE-related pathology damage and decreased the number of Th1s in the periphery, indicating its effectiveness in the treatment of murine EAE. Thus, our findings strongly support the therapeutic potential of this agent as a new traditional Chinese medicine approach in MS treatment.


Assuntos
Cordyceps , Encefalomielite Autoimune Experimental/imunologia , Encefalomielite Autoimune Experimental/metabolismo , Medicina Tradicional Chinesa/métodos , Análise de Variância , Animais , Linfócitos T CD4-Positivos/metabolismo , Modelos Animais de Doenças , Encefalomielite Autoimune Experimental/tratamento farmacológico , Feminino , Citometria de Fluxo , Camundongos , Camundongos Endogâmicos C57BL , Projetos Piloto , Células Th1/metabolismo , Células Th17/metabolismo
8.
PLoS One ; 12(8): e0184033, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28850606

RESUMO

Anthocyanins are the polyphenolic phytochemicals which have been shown to scavenge free radicals. In this study, we investigated the effects of anthocyanins extracted from red-fleshed apples (Malus sieversii) on reducing oxidative damage by Rosup in porcine granulosa cells (GCs) by measuring intracellular reactive oxygen species (ROS), content of glutathione (GSH), activities of superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPX1) and the gene expression of SOD1, CAT, GPX1. Apoptosis was determined with TdT-mediated dUTP-biotin nick end labeling (TUNEL) and apoptosis-related proteins were quantified with Western blotting. The results indicate that Rosup increases oxidative stress by inducing reactive oxygen species production in porcine GCs and the oxidative stress could be reduced by anthocyanins. The gene expression of SOD1, CAT, GPX1 and the activities of these enzymes were increased when GCs were treated with anthocyanins and Rosup for 6 hours. Anthocyanins inhibit Rosup-induced apoptosis by increasing expression of antiapoptotic protein Bcl-2 and suppressing the expression of pro-apoptotic protein Bax. Collectively, anthocyanins from red-fleshed apples reduce oxidative stress and inhibit apoptosis in porcine GCs in vitro. This approach indicates that antioxidants might be developed from red-fleshed apples.


Assuntos
Antocianinas/farmacologia , Antioxidantes/farmacologia , Células da Granulosa/efeitos dos fármacos , Malus , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Apoptose/efeitos dos fármacos , Catalase/genética , Catalase/metabolismo , Feminino , Glutationa/metabolismo , Glutationa Peroxidase/genética , Glutationa Peroxidase/metabolismo , Células da Granulosa/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Suínos
9.
Physiol Res ; 66(5): 715-727, 2017 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-28730825

RESUMO

Fragile X syndrome (FXS) is the most frequently inherited form of intellectual disability and prevalent single-gene cause of autism. A priority of FXS research is to determine the molecular mechanisms underlying the cognitive and social functioning impairments in humans and the FXS mouse model. Glutamate ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (AMPARs) mediate a majority of fast excitatory neurotransmission in the central nervous system and are critically important for nearly all aspects of brain function, including neuronal development, synaptic plasticity, and learning and memory. Both preclinical and clinical studies have indicated that expression, trafficking, and functions of AMPARs are altered and result in altered synapse development and plasticity, cognitive impairment, and poor mental health in FXS. In this review, we discuss the contribution of AMPARs to disorders of FXS by highlighting recent research advances with a specific focus on change in AMPARs expression, trafficking, and dependent synaptic plasticity. Since changes in synaptic strength underlie the basis of learning, development, and disease, we suggest that the current knowledge base of AMPARs has reached a unique point to permit a comprehensive re-evaluation of their roles in FXS.


Assuntos
Síndrome do Cromossomo X Frágil/metabolismo , Deficiência Intelectual/metabolismo , Plasticidade Neuronal/fisiologia , Receptores de AMPA/metabolismo , Animais , Síndrome do Cromossomo X Frágil/genética , Humanos , Deficiência Intelectual/genética , Mutação/fisiologia , Transporte Proteico/fisiologia , Receptores de AMPA/genética
10.
Eur Neurol ; 77(1-2): 80-86, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27960165

RESUMO

BACKGROUND: CT perfusion (CTP) imaging provides quantitative evaluation of cerebral perfusion flow and volume. Our previous findings showed that benign oligemia caused by intracranial large artery disease may be existent in subacute stroke. AIMS: We aimed at comparing the topographic patterns and clinical outcome of stroke patients with and without persistent benign oligemia as defined by CTP imaging. METHODS: Consecutive ischemic stroke patients who were referred for CTP in 2009 were screened. The topographic patterns (cortical, borderzone or perforating artery territory infarcts) were assessed by diffusion-weighted imaging (DWI). The clinical outcome was defined by modified Rankin score at 6 months after stroke onset. RESULTS: Totally, 26 stroke patients were recruited. Benign oligemia in subacute stage was detected in 15 patients. The occurrence of borderzone infarction was higher in stroke patients with benign oligemia than those without (p = 0.036). CONCLUSIONS: The topographic pattern of DWI may be different between the intracranial arterial disease patients with and without benign oligemia on CTP in subacute stroke.


Assuntos
Infarto/patologia , Acidente Vascular Cerebral/patologia , Idoso , Idoso de 80 Anos ou mais , Imagem de Difusão por Ressonância Magnética , Feminino , Humanos , Infarto/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Imagem de Perfusão , Estudos Retrospectivos , Acidente Vascular Cerebral/diagnóstico por imagem
11.
Postepy Kardiol Interwencyjnej ; 12(1): 25-31, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26966446

RESUMO

INTRODUCTION: Noninvasive fractional flow reserve (FFR) computed from CT (FFRCT) is a novel method for determining the physiologic significance of coronary artery disease (CAD). Several clinical trials have been conducted, but its diagnostic performance varied among different trials. AIM: To determine the cut-off value of FFRCT and its correlation with the gold standard used to diagnose CAD in clinical practice. MATERIAL AND METHODS: Forty patients with single vessel disease were included in our study. Computed tomography scan and coronary angiography with FFR were conducted for these patients. Three-dimensional geometric reconstruction and numerical analysis based on the computed tomographic angiogram (CTA) of coronary arteries were applied to obtain the values of FFRCT. The correlation between FFRCT and the gold standard used in clinical practice was tested. RESULTS: For FFRCT, the best cut-off value was 0.76, with the sensitivity, specificity, positive predictive value and negative predictive values of 84.6%, 92.9%, 88% and 73.3%, respectively. The area under the receiver-operator characteristics curve was 0.945 (p < 0.0001). There was a good correlation of FFRCT values with FFR values (r = 0.94, p < 0.0001), with a slight overestimation of FFRCT as compared with measured FFR (mean difference 0.01 ±0.11, p < 0.05). For inter-observer agreement, the mean κ value was 0.69 (0.61 to 0.78) and for intra-observer agreement the mean κ value was 0.61 (0.50 to 0.72). CONCLUSIONS: FFRCT derived from CT of the coronary artery is a reliable non-invasive way providing reliable functional information of coronary artery stenosis.

12.
Physiol Plant ; 157(4): 490-506, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26847714

RESUMO

ARGONAUTE7 (AGO7), a key regulator of the trans-acting small interfering RNAs (ta-siRNA) pathway, plays a conserved role in controlling leaf pattern among species. However, little is known about the ta-siRNA pathway in regulating inflorescence architecture and fruit yield. In this study, we characterized the expression pattern, subcellular localization and developmental functions of SlAGO7 in tomato (Solanum lycopersicum). Overexpressing SlAGO7 in tomato exhibited pleiotropic phenotypes, including improved axillary bud formation, altered leaf morphology and inflorescence architecture, and increased fruit yield. Cross-sectioning of leaves showed that the number of vascular bundles was significantly increased in 35:SlAGO7 lines. Overexpression of SlAGO7 increased the production of ta-siRNA, and repressed the expression ta-siRNA-targeted genes (SlARF2a, SlARF2b, SlARF3 and SlARF4). Further analysis showed that overexpression of SlAGO7 alters the expression of key genes implicated in leaf morphology, inflorescence architecture, auxin transport and signaling. In addition, the altered auxin response of 35:SlAGO7 lines were also investigated. These results suggested that SlAGO7 plays a positive role in determining inflorescence architecture and fruit yield though the ta-siRNA pathway. Therefore, SlAGO7 represents a useful gene that can be incorporated in tomato breeding programs for developing cultivars with yield potential.


Assuntos
Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/genética , Reguladores de Crescimento de Planta/metabolismo , Proteínas de Plantas/metabolismo , Sequência de Bases , Expressão Ectópica do Gene , Frutas/anatomia & histologia , Frutas/genética , Frutas/crescimento & desenvolvimento , Ácidos Indolacéticos/metabolismo , Inflorescência/anatomia & histologia , Inflorescência/genética , Inflorescência/crescimento & desenvolvimento , Lycopersicon esculentum/anatomia & histologia , Lycopersicon esculentum/crescimento & desenvolvimento , Fenótipo , Folhas de Planta/anatomia & histologia , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , RNA Interferente Pequeno , Alinhamento de Sequência
13.
Transl Neurosci ; 7(1): 76-83, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28123825

RESUMO

Multiple sclerosis (MS) and neuromyelitis optica (NMO) are inflammatory demyelinating disorders of the central nervous system (CNS). Various genetic and environmental factors have been identified to contribute to etiology of MS and NMO. Aquaporin 4 (AQP4), is the most abundant water channel in CNS. AQP4 is expressed in astrocytes of the brain, spinal cord, optic nerve and supportive cells in sensory organs. In contrast to MS, immunoreactivity of AQP4 is abolished in NMO lesions. However, conflicting results have been reported regarding the association between AQP4 polymorphisms and demyelinating disorders. Considering the ethnic differences of genetic variations, replications in other cohorts are required. In this study, single nucleotide polymorphisms (SNPs) of AQP4 gene in patients with NMO/neuromyelitis optica spectrum disorders (NMOSD), and MS in the Northern Han Chinese population were examined. Six selected AQP4 SNPs were genotyped by high-resolution melting (HRM) method. Compared with healthy control (HC), there was no significant difference of AQP4 allele and genotype frequency in MS or NMO/NMOSD group. This study showed no significant association of common AQP4 SNPs with MS or NMO/NMOSD, strongly suggesting that polymorphisms of AQP4 gene are unlikely to confer MS or NMO/NMOSD susceptibility, at least in Northern Han Chinese population.

14.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 28(2): 145-8, 2010 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-20480655

RESUMO

OBJECTIVE: To analyze the influence of four types (class II long elastic, class II short elastic, class III long elastic, class III short elastic) of inter-arch elastics on the stress distribution and displacement of temporomandibular joint (TMJ). METHODS: To reconstruct a three-dimensional element model of left craniofacial bones with TMJ, maxilla, mandible and dentition with MBT straight wire appliance by CT images, Mimics, Ansys software. The influence of four types elastic on the model was analyzed by simulating the clinical conditions. RESULTS: The stress distribution and displacement of TMJ under four types of elastics have been acquired. The stress distributed rule of condition 1 and 2 was similar, compression stress centred on the frontal surface of the condyle, while stretching stress centred on the posterior surface. The stress distributed rule of condition 3 and 4 was similar too, the frontal surface of the condyle was most stretching stress, while posterior surface was most compression stress. The displacement of the condyle of condition 1 and 2 was overall forward and downward with a clockwise rotation, the displacement of the condyle of condition 3 and 4 was overall backward and upward with a anticlockwise rotation. CONCLUSION: Class II long elastic and short elastic, class III long elastic and short elastic, the stress distributed rule was similar, the strength under short elastics was gentle under the same strength value. Overall displacement was downward and forward under class II elastics, the condyloid made a clockwise rotation. Overall displacement was upward and backward under class III elastics, the condyloid made a counterclockwise rotation.


Assuntos
Análise do Estresse Dentário , Articulação Temporomandibular , Análise de Elementos Finitos , Humanos , Mandíbula , Maxila
15.
Ying Yong Sheng Tai Xue Bao ; 20(4): 836-42, 2009 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-19565764

RESUMO

Liriomyza sativae Blanchard (Diptera: Agromyzidae) is one of the important pests harming a wide variety of vegetables and ornamental plants throughout the world. The leaf ethanol extract of Momordica charantia at the concentration of 2000-4000 microg x ml(-1) displayed significant antifeedant and antioviposition activities against L. sativae adults. For further purifying the extract, four solvents, i. e., cyclohexane, ethyl acetate, n-butanol and water, were used to extract the ethanol extract, and the antifeedant and antioviposition activities of the extracts against L. sativae adults were tested. The results showed that after treated with the extracts at the concentration of 1000 microg x ml(-1) for 2 days, the antifeedant index (AFI) of cyclohexane-, ethyl acetate-, n-butanol- and water extracts against L. sativae adults was 11.08%, 34.89%, 22.99% and 0, and the antioviposition index (AOI) was 0, 30.91%, 6.45% and 0, respectively. Ethyl acetate extract had the highest bioactivity. At the concentration of 4000 microg x ml(-1), the AFI and AOI of ethyl acetate extract were 70.95% and 69.49%, respectively. The ethyl acetate extract was then isolated by silica gel column chromatography, and three compounds, i.e., (19S, 23E)-5beta,19-epoxy-19-methoxy-cucurbita-6,23-dien-3beta and 25-diol (compound 1), (19R, 23E)-5beta,19-epoxy-19-methoxy-cucurbita-6,23-dien-3beta and 25-diol (compound 2), and 3beta, 7beta,25-trihydroxycucurbita-5,23-dien-19-al-3-O-beta-D-glucopyranoside (compound 3), were obtained. These three compounds at concentration of 100-400 microg x ml(-1) all had inhibitory effects on the feeding and oviposition of L. sativae. At the concentration of 400 microg x ml(-1), the AFI and AOI were 66.89% , 53.53% and 78.02% , and 76.32%, 58.36% and 78.36% for compound 1, 2 and 3, respectively.


Assuntos
Dípteros/efeitos dos fármacos , Comportamento Alimentar/efeitos dos fármacos , Momordica charantia/química , Oviposição/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Dípteros/fisiologia , Folhas de Planta/química , Triterpenos/isolamento & purificação , Triterpenos/farmacologia
16.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 44(1): 11-4, 2009 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-19489251

RESUMO

OBJECTIVE: To investigate the prevalence and configuration of the isthmuses in the apical 6 mm of the mesial and distal roots of Chinese mandibular first molar by means of micro-computed tomography. METHODS: Thirty-six extracted human mandibular first molars were selected. Specimens were subject to micro-CT and a slice thickness of 30 microm was obtained in the apical 6 mm of the roots examined. The number of sections showing isthmuses at each apical level was recorded. Three-dimensional images of isthmuses of mandibular first molars were reconstructed and observed. RESULTS: The mesial roots of human mandibular first molars had a high incidence of isthmus. The isthmus incidence was greatest 4-6 mm from the apex in human mandibular first molar, with prevalence figures of 49.5%-66.1% and 17.3%-17.8% in mesial and distal roots, respectively. The chi-square test indicated a significant difference in the distribution of isthmuses between the two roots (P < 0.01). CONCLUSIONS: The mesial roots of human mandibular first molars have a high incidence of isthmus, which may have clinical implications especially when surgical endodontics is performed on the mesial roots of mandibular molars.


Assuntos
Cavidade Pulpar/diagnóstico por imagem , Dente Molar/diagnóstico por imagem , Raiz Dentária/diagnóstico por imagem , Microtomografia por Raio-X , Adulto , Humanos , Imageamento Tridimensional , Mandíbula/diagnóstico por imagem , Tratamento do Canal Radicular
17.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 32(6): 978-84, 2007 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-18182713

RESUMO

OBJECTIVE: To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis. METHODS: Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot. RESULTS: 2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis. CONCLUSION: Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.


Assuntos
Neoplasias Nasofaríngeas/metabolismo , Proteoma/metabolismo , Carcinoma , Linhagem Celular Tumoral , Eletroforese em Gel Bidimensional , Humanos , Espectrometria de Massas , Carcinoma Nasofaríngeo , Proteômica
18.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 32(6): 1020-5, 2007 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-18182720

RESUMO

OBJECTIVE: To investigate the expression of haptoglobin in the lesions of condyloma acuminatum (CA) at the mRNA and protein level, and to explore its role in the pathogenesis of CA. METHODS: The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC), Western blot, and hybridization in situ. RESULTS: The in situ hybridization study showed that haptoglobin mRNA was expressed in the epidermal cells in the lesions of CA. The distribution of haptoglobin mRNA expression in the lesions of CA was similar to that of the normal controls, and the expression of haptoglobin mRNA in CA was higher than that of the normal controls. There was a significant difference in the positive expression of haptoglobin mRNA between the CA group and the control group (P<0.05). The immunohistochemical study showed that haptoglobin protein was expressed in the whole layers of epidermal keratinocytes in the lesions of CA at a high level and stronger staining was seen in the stratum basale and stratum spinosum. Haptoglobin protein was expressed predominantly in the stratum basale in normal skin tissues, while weak staining was seen below the stratum spinosum.There was a significant difference in the mean gray value between the CA group and control group (P<0.05). Western blot showed that the haptoglobin expression in CA lesions significantly increased compared with the normal skins (P<0.05). CONCLUSION: The expression of haptoglobin mRNA in the CA lesions obviously increases and the epidermal cells in the CA lesions are able to synthesize haptoglobin protein. Haptoglobin in the CA lesions may involve in the local immunity escape by preventing Langerhans cell functional maturation and inhibiting the immunocompetence of keratinocyte.


Assuntos
Condiloma Acuminado/metabolismo , Haptoglobinas/metabolismo , Adolescente , Adulto , Idoso , Western Blotting , Estudos de Casos e Controles , Condiloma Acuminado/genética , Células Epidérmicas , Haptoglobinas/genética , Humanos , Hibridização In Situ , Queratinócitos/metabolismo , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , Adulto Jovem
19.
Nan Fang Yi Ke Da Xue Xue Bao ; 26(6): 831-2, 836, 2006 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16793613

RESUMO

OBJECTIVE: To survey mycoplasma infection in female urogenital tract and analyze the drug sensitivity of mycoplasma in Changsha. METHODS: Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) were detected in 6566 cases of female urogenital tract infection by means of mycoplasma culture and drug sensitivity reagent kit. Sensitivity tests for 10 antibiotics were also performed. RESULTS: A total of 2938 cases were mycoplasma-positive (positivity rate of 44.75%), including 2469 Uu-positive cases (37.6%), 52 Mh-positive cases (0.08%) and 417 cases positive for both Uu and Mh (6.35%). Josamycin, doxycycline, clarithromycin and azithromycin were more effective against Uu infection. Josamycin, doxycycline and thiamphenicol were more effective against Mh infection. Mixed infection with Uu and Mh was more resistant to most antibiotics but Josamycin and doxycycline. CONCLUSION: The female urogenital mycoplasma infection results mainly from Uu. Compared with simple Uu or Mh infection, mixed infection with Uu and Mh has significantly greater resistance to a wider variety of drugs. Josamycin and doxycycline are the primary choice for female urogenital mycoplasma infection in Changsha.


Assuntos
Infecções por Mycoplasma/microbiologia , Mycoplasma hominis/efeitos dos fármacos , Infecções por Ureaplasma/microbiologia , Ureaplasma urealyticum/efeitos dos fármacos , Cervicite Uterina/microbiologia , Adolescente , Adulto , Idoso , China/epidemiologia , Doxiciclina/farmacologia , Doxiciclina/uso terapêutico , Farmacorresistência Bacteriana , Feminino , Humanos , Josamicina/farmacologia , Josamicina/uso terapêutico , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Infecções por Mycoplasma/tratamento farmacológico , Infecções por Mycoplasma/epidemiologia , Infecções por Ureaplasma/tratamento farmacológico , Infecções por Ureaplasma/epidemiologia
20.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 39(5): 379-81, 2004 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-15498342

RESUMO

OBJECTIVE: To evaluate the clinical effect of microsonic technique on removal of intracanal separated instruments. METHODS: Forty-four teeth with 47 intracanal separated instruments, which could not be successfully managed by conventional methods, were endodontically treated with ultrasonic instruments under dental operating microscope. The success rate was calculated and the cases were reviewed 5 to 19 months after retreatment. RESULTS: Thirty-four (34/47) separated instruments in 33 (33/44) teeth were completely removed with a success rate of 75%. Thirteen separated instruments in 11 teeth, however, could not be eliminated from the canals. Root canal perforation or vertical fracture was not found. None of these teeth showed periapical deterioration in the follow-up. CONCLUSIONS: The combined use of dental operating microscope and ultrasonic instruments in endodontic treatment enriched the way of managing complicated cases of intracanal separated instruments.


Assuntos
Cavidade Pulpar/ultraestrutura , Corpos Estranhos/terapia , Terapia por Ultrassom , Adulto , Idoso , Instrumentos Odontológicos/efeitos adversos , Feminino , Seguimentos , Humanos , Masculino , Microscopia , Pessoa de Meia-Idade , Resultado do Tratamento
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