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1.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-744763

RESUMO

Objective To address the mediating role of cognitive emotion regulation in the relationship between parenting styles and health risk behavior in left-behind children.Methods A total of 404 leftbehind children were surveyed with the egna minnen av barndoms uppfostran (EMBU),the cognitive emotion regulation questionnaire-Chinese version (CERQ-C) and the adolescent health related risky behavior inventory (AHRBI).Results (1) The whole average score of AHRBI was (1.47±0.24);the scores of positive and negative cognitive emotion regulation respectively were (10.52±2.56) and (9.51±2.55);the scores of Authoritative,authoritarian and permissive/neglecting parenting styles respectively were (44.06± 8.50),(18.89±3.44) and (10.15±2.32).(2)The health risk behaviors of left-behind children were negatively related with authoritative parenting style (r=-0.26 ~-0.46,P<0.01) and positive cognitive emotion regulation(r=-0.19~-0.44,P<0.01),and positively related with authoritarian parenting style,permissive/neglecting parenting styles(r=0.19 ~ 0.40,P<0.05) and negative cognitive emotion regulation (r=0.25 ~0.51,P<0.05).(3) Authoritative parenting style was positively related with positive cognitive emotion regulation (r=0.30 ~ 0.47,P<0.01),and negatively related with negative cognitive emotion regulation (r=-0.21 ~-0.30,P<0.01),while authoritarian and permissive/neglecting parenting styles were negatively related with positive cognitive emotion regulation (r=-0.11 ~-0.16,P< 0.05),and positively related with negative cognitive emotion regulation (r=0.12~0.40,P<0.05).(4)The mediating effect of cognitive emotion regulation between Authoritative,authoritarian and permissive/neglecting parenting styles and the health risk behaviors of left-behind children respectively were 0.62(87%),0.40(75%) and 0.48(60%).Conclusion The results suggests that parenting styles impact the health risk behavior in left-behind children mainly via the mediating effect of cognitive emotion regulation.

2.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-704187

RESUMO

Objective To explore the status of health risk behaviors and its relationships with child-hood trauma and cognitive emotion regulation in rural orphans. Methods Totally 56 orphans and 56 non-or-phans in rural middle school were surveyed with the adolescent health related risky behavior inventory (AHRBI),the childhood trauma questionnaire (CTQ) and the cognitive emotion regulation questionnaire-Chinese version ( CERQ-C) . Results ( 1) Orphans performed significant higher scores in aggression&vi-olence,rule & law breaking,suicide & self-injury,smoking & drinking,health-compromising behaviors and uprotected sex of AHRBI (1. 67±0. 64,1. 63±0. 58,1. 53±0. 67,1. 75±0. 98,2. 33±0. 59,1. 28±0. 40,re-spectively) than non-orphans (1. 44±0. 36,1. 40±0. 29,1. 31±0. 36,1. 38±0. 48,2. 12±0. 43,1. 13±0. 19, respectively)(P<0. 05). (2) Orphans performed significant higher scores in emotional abuse,physical a-buse,emotional neglect and physical neglect (1. 88±0. 69,1. 62±0. 72,2. 89±0. 94,2. 33±0. 45,respective-ly) than non-orphans (1. 58±0. 67,1. 31±0. 57,2. 41±1. 07,2. 06±0. 68,respectively) (P<0. 05). (3) Orphans performed significant higher scores in self-blame and catastrophizing (12. 34±4. 41,10. 25±4. 14) than non-orphans (10. 86±2. 42,8. 36±3. 43) (P<0. 05),while lower scores in positive refocusing and posi-tive reappraisal (11. 27±3. 50,12. 55±4. 75) than non-orphans (12. 63±3. 54,14. 45±3. 40) (P<0. 05).( 4) The structural equation models which the cognitive emotion regulation mediated the relationship between health risk behavior and childhood trauma fitted well,and the mediating effects of positive regulation and neg-ative regulation respectively accounted for 15. 41% and 68. 92% of the total effects. Conclusion The cur-rent study suggests that rural orphans tend to adopt negative cognitive emotion regulations to copy with their childhood traumas,thus increasing their risk of health risk behaviors.

3.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-657234

RESUMO

Objective To explore the application value of respiratory syncytial virus RNA (RSV-RNA) and respiratory syncytial virus IgM (RSV-IgM) antibody in the diagnosis of children with respiratory infection .Methods From September 2015 to April 2017 ,56 cases of respiratory syncytial virus infection were selected ,and the specimens of pharyngeal secretions were collected ,and RSV-RNA was detected ,in addition ,fasting venous blood was taken from children ,and the RSV-IgM antibody was detected .The positive rate of detection and correlation with age and time of onset were statistically analyzed .Results The positive rates of RSV-RNA and RSV-IgM were 89 .29% and 32 .14% ,respectively .The detection rate of RSV-RNA was significantly higher than that of RSV-IgM ,and the difference was statistically significant (P<0 .05) .The two groups ,≤6 months and from >6 months to 1 year old ,the detection rates of RSV-RNA in children was higher than RSV-IgM (P<0 .05) ,and the detection rate of RSV-RNA in chil-dren with ≤7 days of onset was higher than that of RSV-IgM (P<0 .05) .Conclusion The detection of children with RSV infec-tion ,which the detection of RNA has higher accuracy than IgM ,especially for children ≤1 year old ,the onset time is shorter(≤7 days) ,plays an important guiding role for early diagnosis .

4.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-659088

RESUMO

Objective To explore the application value of respiratory syncytial virus RNA (RSV-RNA) and respiratory syncytial virus IgM (RSV-IgM) antibody in the diagnosis of children with respiratory infection .Methods From September 2015 to April 2017 ,56 cases of respiratory syncytial virus infection were selected ,and the specimens of pharyngeal secretions were collected ,and RSV-RNA was detected ,in addition ,fasting venous blood was taken from children ,and the RSV-IgM antibody was detected .The positive rate of detection and correlation with age and time of onset were statistically analyzed .Results The positive rates of RSV-RNA and RSV-IgM were 89 .29% and 32 .14% ,respectively .The detection rate of RSV-RNA was significantly higher than that of RSV-IgM ,and the difference was statistically significant (P<0 .05) .The two groups ,≤6 months and from >6 months to 1 year old ,the detection rates of RSV-RNA in children was higher than RSV-IgM (P<0 .05) ,and the detection rate of RSV-RNA in chil-dren with ≤7 days of onset was higher than that of RSV-IgM (P<0 .05) .Conclusion The detection of children with RSV infec-tion ,which the detection of RNA has higher accuracy than IgM ,especially for children ≤1 year old ,the onset time is shorter(≤7 days) ,plays an important guiding role for early diagnosis .

5.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-494062

RESUMO

Objective To investigate the symptoms and the needs of transitional care for the patients with lung cancer at the intermission of chemotherapy and provide references for the symptom management and carrying out transitional care. Methods Totally 156 patients with lung cancer were investigated by questionnaire survey. Results With the increase of chemotherapy, symptoms such as cough, sputum, dyspnea were relieved after chemotherapy(F=18.266, 20.463, 17.371, P 0.05). 79.49%(124/156)patients did not know the content and function of transitional care, 98.72%(154/156)patients hope to get transitional care through the hospital. Conclusions Symptoms by side effects were getting worse for patients with lung cancer and influenced their daily life. Patients got to know less about transitional care, however they had a strong need for transitional care. We should reinforce the management of symptoms and transitional care to improve their quality of life.

6.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 27(12): 970-4, 2015 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-27318339

RESUMO

OBJECTIVE: To investigate the predictive value of 4,183 Da peptide of dermcidin protein in the early diagnosis and differential diagnosis of ischemic heart disease. METHODS: A prospective controlled study was conducted. Serum samples were drawn from 161 patients with acute coronary'syndrome [ACS, including 46 patients with unstable angina (UA), 23 with acute non-ST elevation myocardial infarction, and 92 with acute ST segment elevation myocardial infarction], 111 subjects for routine physical examination, including 45 patients with hypertension history, 42 with coronary heart disease, 22 with diabetes, and 54 patients with non-ACS (including pulmonary embolism, aortic dissection, aneurysm, arrhythmia, myocarditis, coronary myocardial bridge, pleurisy, pneumothorax pneumomediastinum, rib fracture, reflux esophagitis, peptic ulcer, and pancreatitis) to serve as controls. 4 183 Da peptide of dermcidin protein was assessed with matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology, and myeloperoxidase [MPO, determined by point-of-care testing (POCT) and enzyme linked immunosorbent assay (ELISA), respectively], high sensitive C-reactive protein (hs-CRP), heart type fatty acid binding protein (H-FABP), myoglobin (MYO), cardiac troponin I (cTnI), and MB isoenzyme of creatine kinase (CK-MB) were quantitated with biochemical analysis. The power of the biomarkers above for early diagnosis and differential diagnosis for ischemic heart disease were judged by comparison of their sensitivity and specificity. RESULTS: (1) It was showed by one-way ANOVA that 4,183 Da peptide was higher in ACS group than that in control group (relative abundance: 22.05 ± 16.97 vs. 15.52 ± 14.09, P = 0.001), but no difference was found between ACS group and non-ACS group (relative abundance: 22.05 ± 16.97 vs. 19.99 ± 17.63, P = 0.416). (2) The specificity and sensitivity of the 4 183 Da polypeptide and MPO for predicting ACS and UA were compared with the receiver operating characteristic curve (ROC). It was showed that the 4,183 Da polypeptide had predictive values for ACS and UA, and the areas under the ROC curve (AUC) was 0.625 and 0.651 (both P < 0.01), but MPO was not found to have predictive value (AUC was 0.440 and 0.336, respectively, both P > 0.05). (3) It was showed by the values of multi-markers in differential diagnosis of ACS and non-ACS disease that the specificity and sensitivity of 4 183 Da peptide in the differential diagnosis of acute myocardial infarction (AMI) and non-ACS disease were less than those of MYO, cTnI, H-FABP, markers of myocardial damage, which AUCs were 0.569 vs. 0.796, 0.833, 0.838, and equal to MPO (POCT/ELISA) and hs-CRP, AUC of which was 0.569 vs. 0.505 (POCT)/0.477 (ELISA) and 0.545. But both the value of 4 183 Da peptide and MYO, cTnI, H-FABP in the differential diagnosis of UA and non-ACS disease was not found, where AUC was 0.456, 0.525, 0.658, 0.568. CONCLUSION: 4,183 Da polypeptide, a fragment of dermcidin protein, may have association with the onset of ischemic heart disease, and may be helpful in the early diagnosis of ACS.


Assuntos
Síndrome Coronariana Aguda/sangue , Síndrome Coronariana Aguda/diagnóstico , Peptídeos/sangue , Doença Aguda , Angina Instável/sangue , Área Sob a Curva , Biomarcadores , Proteína C-Reativa/análise , Creatina Quinase Forma MB/sangue , Diagnóstico Diferencial , Diagnóstico Precoce , Proteína 3 Ligante de Ácido Graxo , Proteínas de Ligação a Ácido Graxo/sangue , Humanos , Infarto do Miocárdio/sangue , Miocárdio , Mioglobina/sangue , Estudos Prospectivos , Curva ROC , Sensibilidade e Especificidade , Fatores de Tempo , Troponina I/sangue
7.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-602123

RESUMO

Objective Pseudomonasaeruginosa resistance to quinolones and around symbiotic bacteria resistant plasmids each chromosome metastasis.Methods 481 samples was collected from the Seventh People’s Hospital of Shenzhen since January 2011 to December 2013,and it cultured Pseudomonasaeruginosa 31 cases,susceptibility testing confirmed 15 cases of quin-olone-resistant as the research obj ect,using plasmid transformation,pick up experiments confirmed the presence of the re-sistance plasmid,PCR amplification,gene sequence analysis,and gene sequences surrounding the symbiotic bacteria resistant plasmids as a same.Results The same gene sequence of plasmid was found between drug-resistant strains of Pseudomonas aeruginosa and the surrounding symbiotic bacteria[χ2=1.207,P<0.01].Conclusion Resistance plasmids could be trans-ferred between different species of bacteria.

8.
Chinese Critical Care Medicine ; (12): 970-974, 2015.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-488362

RESUMO

Objective To investigate the predictive value of 4 183 Da peptide of dermcidin protein in the early diagnosis and differential diagnosis of ischemic heart disease.Methods A prospective controlled study was conducted.Serum samples were drawn from 161 patients with acute coronary syndrome [ACS,including 46 patients with unstable angina (UA),23 with acute non-ST elevation myocardial infarction,and 92 with acute ST segment elevation myocardial infarction],111 subjects for routine physical examination,including 45 patients with hypertension history,42 with coronary heart disease,22 with diabetes,and 54 patients with non-ACS (including pulmonary embolism,aortic dissection aneurysm,arrhythmia,myocarditis,coronary myocardial bridge,pleurisy,pneumothorax,pneumomediastinum,rib fracture,reflux esophagitis,peptic ulcer,and pancreatitis) to serve as controls.4 183 Da peptide of dermcidin protein was assessed with matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology,and myeloperoxidase [MPO,determined by point-of-care testing (POCT) and enzyme linked i mmunosorbent assay (ELISA),respectively],high sensitive C-reactive protein (hs-CRP),heart type fatty acid binding protein (H-FABP),myoglobin (MYO),cardiac troponin Ⅰ (cTnⅠ),and MB isoenzyme of creatine kinase (CK-MB) were quantitated with biochemical analysis.The power of the biomarkers above for early diagnosis and differential diagnosis for ischemic heart disease were judged by comparison of their sensitivity and specificity.Results ① It was showed by one-way ANOVA that 4 183 Da peptide was higher in ACS group than that in control group (relative abundance:22.05 ± 16.97 vs.15.52 ± 14.09,P =0.001),but no difference was found between ACS group and non-ACS group (relative abundance:22.05 ± 16.97 vs.19.99 ± 17.63,P =0.416).② The specificity and sensitivity of the 4 183 Da polypeptide and MPO for predicting ACS and UA were compared with the receiver operating characteristic curve (ROC).It was showed that the 4 183 Da polypeptide had predictive values for ACS and UA,and the areas under the ROC curve (AUC) was 0.625 and 0.651 (both P < 0.01),but MPO was not found to have predictive value (AUC was 0.440 and 0.336,respectively,both P > 0.05).③ It was showed by the values of multi-markers in differential diagnosis of ACS and non-ACS disease that the specificity and sensitivity of 4 183 Da peptide in the differential diagnosis of acute myocardial infarction (AMI) and non-ACS disease were less than those of MYO,cTnⅠ,H-FABP,markers of myocardial damage,which AUCs were 0.569 vs.0.796,0.833,0.838,and equal to MPO (POCT/ELISA) and hs-CRP,AUC of which was 0.569 vs.0.505 (POCT)/0.477 (ELISA) and 0.545.But both the value of 4 183 Da peptide and MYO,cTnⅠ,H-FABP in the differential diagnosis of UA and non-ACS disease was not found,where AUC was 0.456,0.525,0.658,0.568.Conclusion 4 183 Da polypeptide,a fragment of dermcidin protein,may have association with the onset of ischemic heart disease,and may be helpful in the early diagnosis of ACS.

9.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-481879

RESUMO

Objective To analyze the profile of dermcidin (DCD) changes in different stages of acute coronary syndrome (ACS) by quantifying the serum 4 183Da DCD peptide fragment deriving from different ACS patients treated with early antithrombotic therapy.Methods A total of 118 patients with confirmed diagnosis of ACS were enrolled. Immediately after visiting a doctor, the venous blood was collected and afterwards instantly the patient was given orally 300 mg of aspirin and 300 mg clopidogrel, and according to the patient's condition and the consent of his/her or acknowledgement of family members achieved, emergency percutaneous coronary interference (PCI) or thrombolysis or conservative treatment was adopted separately. After anti-thrombotic treatment, at 2, 4, 6, 8, 10, 12, 16, 20, 24, 32, 40, 48, 60 and 72 hours, venous blood was collected and serum isolated respectively. The concentration of 4 183Da DCD fragment in serum was determined by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Simultaneously, the myoglobin (Myo), cardiac troponin I (cTnI) and MB isoenzyme of creatine kinase (CK-MB) were also detected.Results The mean relative strength of nature logarithmic transformations of 4 183Da DCD fragment of 118 patients with ACS was 2.75±1.02 before treatment on admission, and after intervention therapy (mainly antithrombotic therapy) it was decreased to 1.84±1.19 (P = 0.005) and 1.74±1.12 (P = 0.000) at 2 hours and 4 hours, respectively, and then after 4 hours it was slightly elevated. 4 183Da polypeptide increased earlier than myocardial injury markers.Conclusion Aspirin and clopidogrel can significantly decrease the concentration of 4 183Da DCD peptide fragment in serum in patients with ACS, which indicates that the DCD fragment could be used as one of the indexes for observation on early efficacy of antithrombotic therapy.

10.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-672223

RESUMO

ObjectiveToinvestigatetheplasmidgenechangesinquinolone‐sensitivePseudomonasaeruginosa.Methods 31iso‐lates from January 2011 to December 2013 from various qualified clinical samples in the hospital were collected .In the 31 isolateds , 16 isolates proved sensitive to quinolones by using K‐B method were used as research objects in the study .The isolates growing ou‐side the sensitive ring of ciprofloxacin paper were selected to continuously transferred into other culture dishes until the resistance to quinolones were acquired .Plasmid transformation and extraction were performed on those isolates to confirm the existence of drug‐resistanceplasmidsacquired,andthroughPCRandgenesequenceanalysistodeterminethetypeofplasmids.Results 2iso‐lates of quinolone‐sensitive Pseudomonas aeruginosa acquired drug‐resistance plasmids qnrS and were resistant quinolones induced by continuous transferring for 9 times .Conclusion If antibiotics of inhibitory concentration were often used for the treatment of Pseudomonas aeruginosa infection ,drug‐resistance plasmids were acquired easily .

11.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-443110

RESUMO

Objective To explore the relationship between health-risk behaviors and personality disorders in undergraduates.Methods A total of 290 undergraduates were surveyed with the Adolescent Health Related Risky Behavior Inventory (AHRBI) and the Personality Diagnostic Questionnaire-4+ (PDQ-4+),and the whole data was analyzed by t-test and Pearson's correlation analysis.Results (1) The factors scores of AHRBI were significantly positively related with the factor scores of PDQ-4+ in the undergraduates (r=0.12-0.48,P<0.05).(2) The positive groups of Paranoid,schizoid,schizotypal,histrionic,narcissistic,borderline,antisocial and depressive personality disorders performed higher scores of AHRBI (1.57±0.44,1.63±0.52,1.54±0.40,1.78±0.73,1.54 ±0.41,1.62± 0.41,1.61 ± 0.41,1.54 ± 0.37 respectively) than those of the corresponding negative groups (1.37 ±0.24,1.38±0.26,1.37±0.26,1.38±0.25,1.37±0.25,1.35±0.22,1.35±0.21,1.38±0.27 respectively) (P<0.05).Conclusion Personality disorders are closely related with health-risk behaviors of undergraduates,and different health-risk behavior possesses different vulnerability of personality disorder.

12.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-440278

RESUMO

[Abst ract] Objective To explore the relationship between expression of S100B in hippocampus of depression model rats induced by chronic stress and its depression like behavior,and the antidepressant effect of fluoxetine.Methods 40 rats were put into control group,fluoxetine group,CUS group and CUS plus fluoxetine group,using random number table.Rats in each groups received corresponding treatment.Chronic unpredictable stresses (CUS) were performed on rats for 42 days.Fluoxetine (5 mg/(kg · d)) were delivered to rats by intragastric administration from day 22 to day 42.Then,S100B protein were marked and observed by immunohistochemical method.Open-field test,sucrose consumption and body weight were used to evaluate behavioral changes.Results Scores in behavioral test were reduced significantly by 42 days of stress (main effects of stress,P<0.05).Effects of stress on behavioral scores were reversed by 21 days fluoxetine treatment (interactions,P<0.05).CUS resulted in elevated expression of S100B in CA1,CA3 and DG sub-regions in experimental rats (OD values,CUS,0.331 ±0.01,0.353 ± 0.01,0.381 ± 0.007 ; control,0.238 ± 0.007,0.237 ± 0.010,0.228 ± 0.006.Simple effects of stress,P=0.000; P=0.000; P=0.000).Fluoxetine treatment reversed the elevated expression of S100B in CA1,CA3 and DG sub-regions in model rats (OD values:CUS plus fluoxetine,0.233 ± 0.015,0.240 ± 0.005,0.254± 0.015; fluoxetine,0.241±0.007,0.233±0.013,0.227±0.017; Interactions between fluoxetine and CUS,P=0.000; P=0.000; P=0.000).Conclusion Sub-regional over expression of S 100B in hippocampus is associated with depression like behavior of rats.Reversed S100B expression in these sub-regions is an indicator of effective antidepressant treatment but not a mechanism for it.

13.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-414392

RESUMO

Objective To explore the autobiographical memory characteristics of chronic pain patients and its correlators.Methods Autobiographical memory,pain and emotion of 106 chronic pain patients and 106healthy controls were assessed with the Autobiographical Memory Test (AMT), the Short-form McGill Pain Questionnaire (SF-MPQ), the Pain Self-efficacy Questionaire (PSEQ), Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI).Results Compared to the control group, the clinical group had less specific memory (3.63 ± 1.53, 2.63 ± 1.68, F (1.416) = 7.233, P < 0.01 ) and more overgeneral memory (2.37 ± 1.53,3.37 ±1.68, F (1.416) =7.069, P<0.01 ),but the latency to response was not statistically significantly different between the groups.Duration of pain,frequence of pain and pain self-efficacy were the significant predictors of overgeneral memory,and the regression coefficients were significant (P<0.05 ) ,the multiple regression equation was statistically significant( R = 0.427, R2 = 0.183, F= 2.385, P< 0.05 ).Conclusion The autobiographical memory of chronic pain patients was overgeneralized because of the influence of duration, frequence and self-efficacy of pain.

14.
J Burn Care Res ; 31(2): 297-303, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20182378

RESUMO

The objective of the study is to investigate the effect of fasudil [1-(5-isoquinolinesulfonyl) homopiperazine] (HA1077, calcium antagonist vasodilator and an inhibitor of RhoA kinase) on expression of cytokeratin (CK), DNA multiplication, and synthesis of mesenchymal stem cells (MSC). After rats were killed, MSC were separated from rats and proliferated in culture medium. The cells were randomly divided into control group, HA1077 control group, induction group, and HA1077 group. The percentage of CK19+MSC, proliferating cell nuclear antigen (PCNA), and cell cycle were determined. The test was repeated for five times. SPSS 12 software was used to analyze all the data. A P value <.05 and P < .01 were considered to be significant. The flow cytometry result showed that 1) the isolated MSC were uniformly positive for CD44, SSEA-1, CD105 but were negative for CD34, CD14, and CD45. 2) In the HA1077 control group, the percentage of CK19+MSC was similar to that in control group, which was lower than that in the induction group. In the HA1077 group, the percentage of CK19+MSC was higher than in the induction group (P < .01), in HA1077 control group (P < .01), and in control group (P < .01). 3) PCNA-positive rate in HA1077 group was highest among the groups. PCNA-positive rate of induction group was higher than that of control group and HA1077 control group. The cell cycle analysis showed that the quantity of MSC in S phase of HA1077 group was also highest. The quantity of MSC in S phase of induction group was higher than that of control group and HA1077 control group. There was no difference between the HA1077 control group and control group. HA1077 can repair burn wounds in future by promoting MSC differentiating into epidermal cell through DNA multiplication and synthesis.


Assuntos
1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/análogos & derivados , Queratinas/metabolismo , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/farmacologia , Animais , Antígenos CD/metabolismo , Ciclo Celular , Citometria de Fluxo , Técnicas Imunoenzimáticas , Antígeno Nuclear de Célula em Proliferação/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar , Cicatrização/fisiologia
15.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-814404

RESUMO

OBJECTIVE@#To explore the autobiographical memory characteristics in depressed patients and their influence factors.@*METHODS@#Autobiographical memory, emotion and cognitive executive function of 60 depressed patients and 60 healthy controls were assessed with autobiographical memory test (AMT), Hamilton depression scale (HAMD), Beck depression inventory (BDI), Beck anxiety inventory (BAI), hospital anxiety and depression scale (HAD), arrow-task stroop test (ATST), Wisconsin card sorting test (WCST), Backward masking test (BMT) and continuous performance test (CPT).@*RESULTS@#The specific memory of the depressed group was significantly less than that of the control group, and was negatively related with the negative emotion score, the time of anterograde and retrograde reading of ATST, and the time difference of ATST. The overgeneral memory increased and the latency to response of ATST was significantly longer than that of the control group. The two factors were positively related with the negative emotion score, the time of anterograde and retrograde reading of ATST, and the time difference of ATST.@*CONCLUSION@#The autobiographical memory of the depressed patients is overgeneralized and retarded. These characteristics are related with negative emotion and impairment of cognitive executive function.


Assuntos
Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Estudos de Casos e Controles , Cognição , Fisiologia , Depressão , Psicologia , Memória , Fisiologia , Rememoração Mental , Fisiologia , Escalas de Graduação Psiquiátrica
16.
Chinese Journal of Trauma ; (12): 663-667, 2009.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-393917

RESUMO

Objective To observe the effect and mechanism of HA1077 on bone mesenchymal stem cells (BMSCs) promoting wound healing. Methods (1) In vitro study: rats' BMSCs were har-vested and divided into three groups randomly, ie, control group, induction group and HA1077 group. In control group, culture medium was DMEM with 10% fetal bovine serum. In induction group, induction medium was DMEM which consisted of 10% FBS, 8μg/ml EGF and epithelium culture condition medi-um. In HA1077 group, culture condition medium was the induction medium pins 10 μmol/L HA1077. At day 7 after replanting, CK19 expression, PCNA expression and cell cycle among three groups were clarified by flow cytometry. (2) In vivo study: rabbit's BMSCs were isolated. Six pieces of full skin loss wound were created on the back of a rabbit, with three pieces of wound on the half side of back. The wound was round and 3 cm in diameter. Eight New Zealand rabbits were randomly divided into four groups, ie, control group (Group A), EGF control group (Group B), BMSCs group (Group C) and BMSCs + HA1077 group (Group D). Wounds were treated by normal saline in Group A and by EGF (50 U/cm2) in Group B. Besides EGF,1 ml of BMSCs suspension (2 × 106/ml) was used for wound re-pair in Group C. 0.5 ml HAIO77(20 μmol/L), BMSCs and EGF were used together for wound repair in GronpD. At days3, 7and 14 after injury, wound elosuroindex (WCI) was calculated. Results In vitro study:the positive rate of CK19 in the HA1077 group was (40.31±0.82)%, higher than (11.13±2.14)% in the induction group(P < 0.01). The PCNA positive rate and the S phase, cell quantity in HA1077 group were also higher than that in the induction group. In vivo study: WCI was (86.20±1.92) % in Group D at day 14 after injury, obviously higher than that in other three groups. Conclusions HA1077 can promote BMSCs' entering into S phase, expressing PCNA and differentiating and expressing CK19. In vivo combination of HA1077 with BMSCs may facilitate wound healing.

17.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-396890

RESUMO

Objective To discuss the experience of medical care for the assurance of security of the'Shenzhou Ⅶ'astronauts at the landing venue in order to innovate a high efficient mobile ICU in medical helicopters.Method The data collected from over.as and domestics were reviewed and analyzed to find out the possible causes of accidental injuries to astronauts during acmspaec flight,and to pmpose a practicably and effectively prophylactic strategy and reasonable treatments for the astronauts with accidental injuries.Results Three,high efficient mobile ICU innovated could elqsure the safety ofthe astronauts.The ICUs with high efficiency were set up in the helicopters where the surgery Call he peormed for controlling the danlage.Fine armature made our work mole effective.Conclusions Practicably and effectively Plephylactic measurements and reasonable treatments are the essential aspect of the successfulness for the security of astronauts of manned aemspacecraft flights.The innovated medical care system in helicopters could realized the obligation of swift response and the duty offirst-md.

18.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-585918

RESUMO

OBJECTIVE To reconstruct the HNP-1 into the J-HNP-1 with a J chain,and explore to set up a mammalian cell expression system which can express and secret J-HNP-1,so that the products could be examined and purified conveniently. METHODS The J-HNP-1 cDNA fragments were produced by recombinant PCR.Then the J-HNP-1 was inserted into the mammalian expression vector pcDNA3.1(-)/Myc-His which had the double marks Myc and 6?His.The recombinant vector rpcDNA3.1(-)/Myc-His /J-HNP-1 was transfected into the COS-7 cell.The J-HNP-1 expression was analyzed at the mRNA and protein level.The germicidal activity of cell culture supernatant and cellular solution protein was assayed. RESULTS By the use of RT-PCR with special primers,a band of 786bp was amplified from COS-7 cells transfected by this recombinant plasmid.Western blot analysis with specific anti-histidines antibody revealed that the cell culture supernatant and cellular solution protein of COS-7 cells transfected by rpcDNA3.1(-)/Myc-His /J-HNP-1 had a strong band with relative molecular mass of about 24?10~3.Antibacterial activity assay showed that obvious bacterial inhibition occurred in both lysate and supernatant of COS-7 cells transfected by rpcDNA3.1(-)/Myc-His /J-HNP-1. CONCLUSIONS The J-HNP-1 recombinant is obtained and inserted into the mammalian expression vector then to be expressed in vitro.The expression product is found to have the anti-bacterial effect in vitro.

19.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-586151

RESUMO

OBJECTIVE To recombine J chain gene with HNP-1 into a new germicidal(molecule) J-HNP-1,which can connect with pIgR by J chain,so that by using pIgR as a "bridge" the J-HNP-1(germicidal) peptide can be transported into the epithelial cell of mucous membrane to kill the intracellular microorganisms,then the recombinant is inserted into the mammalian expression system.METHODS The J chain and HNP-1 cDNA were amplified from the plasmids respectively by PCR,then the two cDNA fragments were recombined into J-HNP-1 by recombinant PCR.The J-HNP-1 cDNA fragment was inserted into the(mammalian) expression vector pcDNA3.1(-)/Myc-HisC.RESULTS The J-HNP-1 recombinant was obtained by(connection) of J chain and HNP-1 cDNA by PCR.The recombinant J-HNP-1 cDNA was 786bp.CONCLUSIONS The recombinant J-HNP-1 cDNA and the construction of expression vector are the basis for the new bactericidal peptide production.

20.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-575630

RESUMO

Objective To study the bacteriostatic effect of the volatile oil from Artemisia argyi. Methods Minimal inhibitory concentration (MIC) was measured with double-fold didution method. Protective effect of the volatile oil from Artemisia argyi were determined by experimental treatment of infected mice in vivo. Results The MIC90 of 3 kind of pathogenic bacteria were 0.78~25 ?L/mL and protective rate for infected mice were 40%~50%. Conclusion The volatile oil from Artemisia argyi has better inhibiting effect on Staply lococcus aureus, Escherichiacoli and Pseudomona than Erythromycin. It has better protective effect for infected mice.

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