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1.
Zhongguo Zhong Yao Za Zhi ; 46(18): 4730-4735, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34581082

RESUMO

The present study aimed to explore the mechanism of the sweating of Dipsacus asper on content changes of triterpene sa-ponins by detecting the total triterpene saponins and the index component asperosaponin Ⅵ in the crude and sweated D. asper, and analyzing the differentially expressed proteins by isobaric tags for relative and absolute quantification(iTRAQ) combined with LC-MS/MS. After sweating, the content of total triterpene saponins decreased manifestly, while that of asperosaponin Ⅵ increased significantly. As revealed by the iTRAQ-LC-MS/MS analysis, 140 proteins with significant differential expression were figured out, with 50 up-regulated and 90 down-regulated. GO analysis indicated a variety of hydrolases, oxido-reductases, and transferases in the differential proteins. The results of activity test on two differentially expressed oxido-reductases were consistent with those of the iTRAQ-LC-MS/MS analysis. As demonstrated by the analysis of enzymes related to the triterpene saponin biosynthesis pathway, two enzymes(from CYP450 and UGT families, respectively, which are involved in the structural modification of triterpene saponins) were significantly down-regulated after sweating. The findings suggested that sweating of D. asper presumedly regulated triterpene saponins by affecting the expression of downstream CYP450 s and UGTs in the biosynthesis pathway of triterpene saponins of D. asper.


Assuntos
Dipsacaceae , Saponinas , Triterpenos , Cromatografia Líquida , Humanos , Sudorese , Espectrometria de Massas em Tandem
2.
Zhongguo Zhong Yao Za Zhi ; 45(9): 2036-2041, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495550

RESUMO

Gastrodia elata is a kind of precious traditional Chinese medicine. In artificial cultivation, it has not got rid of its dependence on forest resources. In order to maintain the balance of the ecological system and reduce the waste of resources as much as possible, based on the information from field investigation at many places, this paper introduced the new ecological circulation planting patterns of G. elata, such as "forest-G. elata" supporting planting, G. elata-edible mushroom rotation, forest-G. elata-edible mushroom three-dimensional planting, fungus material classification planting technology, and so on. In this paper, we expounded the ecological problems solved by several planting patterns in G. elata production and analyzed their shortcomings. Finally, based on the exis-ting models, a complete ecological planting system of G. elata was summarized. This planting system emphasizes: ① The follow-up forests should be started before the planting of G. elata. And the economic forests were used to cultivation of G. elata. ② The classified utilization of fungus-growing materials. The leaves were used to cultivate germination bacteria of G. elata, the small branches were used to cultivate protocorm and juvenile tuber, the large branches were used to cultivate immature tuber, and the tree trunk was used to cultivate mature tuber. ③ Recycle utilization G. elata fungus material. The old fungus materials were used to produce strains or cultivate edible fungus. This design project not only solves the problems of the source of G. elata fungus material, the efficient utilization of fungus material and land resources, but also enriches the industrial structure. Using limited time and land resources to obtain greater economic benefits. It has certain guiding significance for poverty alleviation and ecological improvement.


Assuntos
Agaricales , Gastrodia , Bactérias , Medicina Tradicional Chinesa , Tubérculos
3.
Zhongguo Zhong Yao Za Zhi ; 45(9): 2042-2045, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495551

RESUMO

The technique of "simulative habitat cultivation" is to preserve the quality of traditional Chinese medicine by simulating the original habitat and site environment of wild Chinese medicine resources. Dendrobium nobile is the most representative variety of traditional Chinese medicine which reflects the coordinated development of medicinal material production and ecological environment. In this paper, the main technical points of the simulated cultivation model of D. nobile were summarized as follows: rapid propagation of seedling tissue technology to ensure the genetic stability of provenance; line card+fermented cow manure+live moss method to improve the survival rate; epiphytic stone cultivation to improve the quality of medicinal materials; and the integration of mycorrhizal fungi to improve the quality stability of medicinal materials. On the basis of summarizing the ecological benefits, economical and social benefits generated by the application of the technology, the paper systematically analyzes the principle of the technology for the cultivation of D. nobile to promote the excellent quality, the light, gas, heat and fertilizer resources of the undergrowth niche are in line with the wild site environment of D. nobile. The rich and complex soil microbial community in the forest laid the foundation for the species diversity needed for the growth of D. nobile.The stress effect on the growth of D. nobile resulted in the accumulation of secondary metabolites. The symbiotic relationship between the symbiotic fungi such as bryophytes and D. nobile promotes the synthesis of plant secondary metabolites. The high quality D. nobile was produced efficiently by improving and optimizing the cultivation techniques.


Assuntos
Dendrobium , Micorrizas , Animais , Bovinos , Ecossistema , Feminino , Medicina Tradicional Chinesa , Simbiose
4.
Zhongguo Zhong Yao Za Zhi ; 45(3): 457-462, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237500

RESUMO

Gastrodia elata is a kind of precious traditional Chinese medicine. In the process of cultivation of G. elata, due to the influence of continuous cropping obstacles and other factors, the fungus materials and land that have been planted with G. elata are often abandoned, resulting in a great waste of resources. Based on the planting characteristics of G. elata and Phallus impudicus and the previous research experience in ecological agriculture, this paper analyzed the ecological adaptability characteristics of G. elata and P. impudicus, and summarized the key techniques of the G. elata-P. impudicus sequential planting pattern. Keeping track of the planting area, fungus-growing materials consumption and market sales of G. elata-P. impudicus sequential planting pattern, the ecological benefits of G. elata-P. impudicus sequential planting pattern from the aspects of utilization rate of fungus-growing materials were analyzed, the value of land resources per unit area, ecological environmental protection, labor cost and economic benefits were consi-dered. The technical principle of G. elata-P. impudicus sequential planting pattern was expounded according to their ecological habit, the season of harvest and planting, the difference of composition of fungus-growing materials, and the microbial ecology. The sequential planting pattern of G. elata-P. impudicus not only realized the double production of medicinal materials and edible fungi, reduced the waste of old fungus-growing materials, but also transformed the energy from nutrition-supplied fungi to edible and medicinal fungi, which guaranteed the ecological recycling and utilization of G. elata in the process of cultivation.


Assuntos
Agaricales/crescimento & desenvolvimento , Agricultura/métodos , Gastrodia/crescimento & desenvolvimento , Plantas Medicinais/crescimento & desenvolvimento , Medicina Tradicional Chinesa
5.
Zhongguo Zhong Yao Za Zhi ; 45(3): 478-484, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237503

RESUMO

Fungal disease is an important factor restricting the healthy development of Gastrodia elata industry. The control of fungal disease in G. elata is an important issue in production. This paper makes a detailed investigation on the current situation of G. elata disease in China through statistics on the failure rate, rotten pit rate and occurrence rate of G. elata disease in the main producing areas of China. It was found that G. elata disease was mainly infected from the top bud and junction, causing the occurrence rate of disease was 6%-17%, and the yield decreased by 10%-30%. The 23 dominant fungi were isolated from 18 typical G. elata disease samples. Through identification of colony morphology, mycelium morphology, spore morphology and genetic characteristics, they were finally identified as 13 species, belonging to 7 families and 7 genera. Trichoderma harzianum, Ilyonectria sp. and Ilyonectria destructans are the most frequently separated. Their isolation frequency were 22.22%,16.67%,16.67% respectively. Ilyonectria sp. and I. destructans were the first time isolated from G. elata disease samples. They may be the main pathogens causing soil-borne diseases of G. elata. T. harzianum has certain potential as Gastrodia biocontrol bacteria. This study can provide a theoretical basis for the research and development of control technology of Gastrodia fungi disease.


Assuntos
Fungos/patogenicidade , Gastrodia/microbiologia , Doenças das Plantas/microbiologia , China , Fungos/classificação
6.
Zhongguo Zhong Yao Za Zhi ; 44(14): 2996-3001, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602845

RESUMO

Through the records of herbal and market investigations,the samples collected from different market and origin were analyzed,and the appearance character indexes were determined in order to revise the commodity specification and grade standard of Dipsaci Radix combined with production practice. This is also to analyze association of the appearance with quality different of intrinsic components. The investigation results indicated the root's long and thick was better,and atrovirens of fracture surface,there are most of the market is dominated by the ungraded goods. Through principal component analysis,variance analysis and cluster analysis,combining with the feasibility of actual operation and herbal record,the length,middle diameter and phloem color were filtrated and the specification was divided into 2 ranks: the selection and ungraded goods,and the grade was divided into 2 ranks for the selection goods: big,small selected goods. Moreover,there were no significant correlation between the appearance and the intrinsic quality index of Dipsaci Radix,the content of extractum were significantly positive correlation with the moisture,but had extremely negative correlation with the total ash. Multiple comparisons indicated that the content of the moisture,extractum and asperosaponin VI of the selected goods were higher than the ungraded goods,while the total ash content were lower,and they hasn't significant difference. The commodity specification and grade standard of Dipsaci Radix as a basis provide commodity specification and grade standard of communities and standardizing market trade order.


Assuntos
Dipsacaceae/química , Medicamentos de Ervas Chinesas/normas , Raízes de Plantas/química , Análise por Conglomerados , Plantas Medicinais/química , Análise de Componente Principal
7.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3627-3632, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602933

RESUMO

In order to understand the pollution of heavy metals in Guizhou soil planting Eucommia ulmoides,the paper has determined the contents of five heavy metals respectively( Cu,Cd,Pb,Hg and As) by atomic absorption spectrophotometry. The soil environmental quality by single factor pollution index was evaluated,nemerow integrated pollution index and potential ecological risk index,the correlation of 5 heavy metals was analyzed and the relationship between heavy medals content and p H value was discussed. The results showed that: ①The soils of LP,SB and ZA-2 had low heavy metals content,LP soil met the first national standard,and other soils met the second national standard; ②The soils of LP,ZA-1 and ZA-2 were all in a clean state,while HX and BZ-1 were in a light pollution state,and the pollution degree was Cu>Pb>As>Cd>Hg; ③The soil of BZ-2 was light warning( medium ecological risk),the soils of HX,BZ-1 and MT were early warning( low ecological risk),the main warning source was Cu and Pb,and the rest were no warning( no ecological risk); ④Cd and As were positively correlated with Pb and Hg,and Cd,Pb and As were positively correlated with p H. The research showed that the soils of LP,SB,ZA-1 and ZA-2 were clean and could be used for E. ulmoides to continue planting,the soils of HX,MT,BZ-1 and BZ-2 were polluted and should be treated in time by effective measures.


Assuntos
Monitoramento Ambiental , Eucommiaceae , Metais Pesados/análise , Poluentes do Solo/análise , China , Medição de Risco , Solo/química
8.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2219-2225, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359645

RESUMO

The study aims at investigating the grade standard and the quality characteristic of Pinelliae Rhizoma for commodity specification, which provides the reference for its grade standard formulation. 42 Pinelliae Rhizoma simples were collected from 5 medicinal materials markets and 2 producing areas. Based on the previous herbalogical study and market investigation, we combined with the data analysis to select the grading indicators using SPSS software for descriptive statistical analysis, analysis of variance, K-cluster analysis and correlation analysis. According to the actual production condition, we developed the grading standards of Pinelliae Rhizoma. Moreover, we compared the internal indicators(water, total ash, leachate and guanosine) of Pinelliae Rhizoma at various grade levels, and analyzed the correlation between appearance traits and internal indicators. The herbalogical study and market research found that the Pinelliae Rhizoma was better in large, solid and white. The results from descriptive and variance analysis showed that the appearance of Pinelliae Rhizoma was significantly different in weight per grain and grain number of 500 g. Referring to the 2015 Chinese Pharmacopoeia and the production practice, we use the length, weight per grain and grain number of 500 g as the classification index of Pinelliae Rhizoma. The results from correlation analysis showed that there was no significant correlation between the appearance of Pinelliae Rhizoma and the intrinsic quality index. In addition, we found there was no significant difference in the content of the intrinsic index except for the total ash and the extract. The current study established the classification index of the product specification and grade standard of Pinelliae Rhizoma with length, weight per grain and grain number of 500 g as the index, which can provide the basis for the classification of the product specification and grade of Pinelliae Rhizoma market.


Assuntos
Medicamentos de Ervas Chinesas/normas , Pinellia/química , Rizoma/química
9.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2459-2466, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359711

RESUMO

This paper is aimed to study the commodity specification and grade standard of Galla Chinensis,for standardizing market order and guide the market circulation,and provide a basis for standardization of Galla Chinensis. With 33 samples of Galla Chinensis from market as the object of the research,the herbal textual research and market research were carried out. Then the grading indicator were selected by the descriptive statistics,principal component analysis and cluster analysis,combining with production practice,the commodity specification and grade standard of Galla Chinensis were set out. The data of moisture,ash,gallic acid as the internal index were used to analyze the relationship between the quality difference between grades and the appearance characters and the intrinsic composition. Herbal textual research and market research found that the high quality of Galla Chinensis characterized with large,complete,wall thick,grayish brown characteristics,and Galla Chinensis could be divided into gallnuts and horned gall. Through principal component analysis and cluster analysis,combining actual production and herbal record,screening,the length,diameter,single weight,the number of 500 g were determined as 4 grading indicators,the commodity specification was divided into two: gallnuts and horned gall,the grades was divided into two: selected goods and mixed goods. The result of correlation analysis showed there was no significant correlation between the internal index and the appearance characters of Galla Chinensis. The result of multiple comparison showed that the ash content of the selected goods was smaller than that of the mixed goods,but it did not reach significant. The content of gallic acid of the selected goods and the mixed goods of gallnuts were higher than selected goods of horned gall,and higher than mixed goods of horned galls. Using the length,diameter,single weight,the number of 500 g as the appearance characters index. Combining with the herbal textual research and market research,we have divided two specifications and two grades for the commodity specification and grade standard of Galla Chinensis,which can provide a basis for industry standards and national standards.


Assuntos
Medicamentos de Ervas Chinesas/normas , Ácido Gálico/análise , Tumores de Planta , Controle de Qualidade
10.
Zhongguo Zhong Yao Za Zhi ; 44(2): 265-269, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989943

RESUMO

This study is based on the data analysis of medicinal plant resources and diversity collected from the fourth Chinese traditional medicine resource survey( pilot). Through the analysis of relevant data from 33 census pioneer plots in Guizhou province( area),a total of 265 families,1 432 genera and 5 296 species of medicinal resources were reported,including algae,fungi,lichens,mosses,a total of 43 genera and 35 families,57,48 families,120 genera and 453 species of ferns,gymnosperms 11 families,22 genera and 61 species,167 families,1 243 genera and 4 721 species of angiosperms,4 genera and 4 families four medicinal animals.Compared with the data related to the third survey of traditional Chinese medicine resources,the number of ferns,gymnosperms and angiosperms in the fourth survey has increased far more than that of the third survey. From the regional distribution of medicinal resources,the composition of the genus,the type of life,and the location of the medicine,the richness of the medicinal plant resources in Guizhou province is not only reflected in many types,but also in the variety of medicinal resources. These studies provide a scientific basis for vigorously developing the Chinese herbal medicine industry and the sustainably using medicinal plant resources in Guizhou province.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Plantas Medicinais/classificação , China , Cycadopsida , Gleiquênias , Magnoliopsida
11.
Zhongguo Zhong Yao Za Zhi ; 43(21): 4210-4216, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30583619

RESUMO

The study aims at evaluating genetic diversity and medicinal quality of cultivated germplasm in Rehmannia glutinosa, and providing theoretical guidance for screening excellent germplasm. The genetic diversity of 21 species of R. glutinosa were analyzed by SRAP molecular markers, and the catalpol and verbascoside was determined by HPLC. The mass fraction of catalpol and verbascoside in R. glutinosa germplasm were respectively in the range of 2.393%-6.519% and 0.063%-0.478%, the germplasm 14, 16, 15 and 20 germplasm, witch catalpol and verbascoside content was higher. A total of 57 bands were produced by 10 primer, among which 40 polymorphic bands were polymorphic bands, and the percentage of polymorphic loci was 8.77%-54.39%, the Nei's genetic diversity index (H) was 0.374 1, Shannon's polymorphism information index (I) was 0.546 6. Gst and gene flow Nm were 0.608 8 and 0.321 3, respectively. Based on the genetic uniformity, 21 species of germplasm were grouped into 2 categories. The genetic diversity level of R. glutinosa was medium low. The comprehensive consideration of the genetic diversity and the content inculde catalpol and verbascoside, germplasm 7 and germplasm 18 could be used as the preferred materials for the cultivation of reticulum. Germplasm 15 and 16 can be used as the preservation and breeding object of rhubarb germplasm.


Assuntos
Variação Genética , Rehmannia/genética , Animais , Fluxo Gênico , Filogenia , Melhoramento Vegetal , Plantas Medicinais/genética
12.
Zhongguo Zhong Yao Za Zhi ; 42(5): 882-889, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994530

RESUMO

To provide guidance for certification, popularization and application of Pseudostellariae Radix new variety, the regional adaptation and stabilities of "Shitai No.1" were evaluated. The "Qian taizishen No.1" and "SB-C" varieties (strains) were used as the control varieties. The agronomic, medicinal material traits and medicine quality were used as evaluation index to compare the phenotypic difference of the three varieties (strains) in four planting areas. Compared to the control varieties, 10 agronomic traits of "Shitai No.1" had the smallest coefficient of variation among the 18 agronomic traits, and other 8 agronomic traits placed the middle level. Among 8 medicinal material traits and medicine quality indicators, the coefficient of variation of different regions of the extract content, pseudostellarin B content, the number of 50 g root tuber, the plant medicinal materials weight and weight of single root of "Shitai No.1" were the smallest compare to other varieties (strains). It could be divided into three groups based on the phenotypic difference of the three varieties (strains) in four planting areas. The "Shitai No.1" was classified as one group, while the "Qian taizishen No.1" and "SB-C" had cross clustering. The regional stability of several index about agronomic traits, medicinal material traits and medicine quality of "Shitai No.1" were better than that of the control varieties (strains). "Shitai No.1" was suitable for planting, popularization and application in the appropriate ecological areas of Guizhou province.


Assuntos
Caryophyllaceae/química , Medicamentos de Ervas Chinesas/normas , Raízes de Plantas/química , China , Geografia , Peptídeos Cíclicos/análise , Fenótipo , Tubérculos/química , Plantas Medicinais/química
13.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2381-2385, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905556

RESUMO

Based on collections and researches of Pesudostellaria heterophylla germplasm resources from different areas of China, by using Shibing SB-4 provenance as materials, the new variety "Shitai No.1" was bred by mass selection, small plot variety comparative test, regional variety comparative test and field trial planting. Compared with "Qian taizishen No.1" and P. heterophylla land races. The disease and lodging resistance, root yield, polysaccharide content and the first grade rate of "Shitai No.1" have obvious advantages. In addition, it is relatively stable of yield in "Shitai No.1" in different places. It is demonstrated that "Shitai No.1" is a fine variety that adapt to the producing areas of P. heterophylla in Guizhou province, it is worthy to be promoted.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Melhoramento Vegetal , China , Raízes de Plantas , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise
14.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2386-2390, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905557

RESUMO

Maxent model was applied in the study to filtering the climate factors layer by layer. Polysaccharides and pseudostellarin B the two internal quality evaluation index were combined to analyse the interlinkages between climate factors and chemical constituents in order to search for the critical climate factors of Pseudostellaria heterophylla. Then based on the key climate factors to explicit the quality spatial distribution of P. heterophylla. The results showed that polysaccharides and climatic factors had no significant correlation, suggesting that the indicator was not climate-driven metabolites. Pseudostellarin B could construct regression model with the precipitation. And quality regionalization results showed that pseudostellarin B content presented firstly increased and then decreased trend from southeast to northwest, which was the consistent change with precipitation. It clearly proposed that precipitation was the key climate factor, which affected the accumulation of cyclopeptide compound for Pseudostellariae Radix.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Clima , Peptídeos Cíclicos/análise , Raízes de Plantas , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise , Pesquisa
15.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2391-2396, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905558

RESUMO

This paper is aimed to study the potential ecological suitability regionalization of Pseudostellaria heterophylla for selecting GAP planting base location and designing rational production layout. The ecological factors and contribution rate were determined by using maximum entropy (Maxent) model. Then, the information entropy theory was used to determine the relative importance of each environmental factor, and thus to determine the most limiting habitat criteria. Finally, the probable spatial distribution of P. heterophylla was determined based on GIS spatial analysis of habitat conditions. Meanwhile, the optimal index range of ecological factors was quantified. The moderately and highly suitable habitats were mainly located in Shibing, Huangping, Cengong, the middle and east of Kaili, the south of Yuqing, the west of Tongren. The percentage of moderately and highly suitable habitats for P. heterophylla in the study area was 3.64%, and its area was 6 405.39 km². The results also showed that seven dominant ecological factors controlled the distribution of P. heterophylla. These factors included agrotype, the warmest rain, aspect, slope, the warmest and highest temperature, contents of soil organic carbon, and the driest month precipitation. The habitat suitability assessment model based on GIS and Maxent model theory could accurately evaluate the habitat suitability distribution of P. heterophylla in Guizhou. In addition, we recommended Cengong and Zhenyuan county in Guizhou province as the worthy developing potential planting areas.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Ecossistema , China , Ecologia , Entropia , Plantas Medicinais/crescimento & desenvolvimento , Controle de Qualidade , Solo
16.
Zhong Yao Cai ; 38(4): 674-8, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26672330

RESUMO

OBJECTIVE: To study the effect of basic soil nutrients and inorganic elements on the quality of Pseudostellaria heterophylla root, in order to reveal the inner link and to provide the scientific basis for rational cultivation of Pseudostellaria heterophylla. METHODS: The medicinal materials of Pseudostellariae Radix and soil samples from 15 habitats were collected, and three essential nutrients, five inorganic elements in the soil and the medicinal contents of polysaccharides and pseudostellarin B were determined. Then using SPSS software to analyze its relevance. RESULTS: Significant difference of pseudostellarin B content was found in samples from different provinces, which was not detected in the sample of Fujian Province, but the difference of polysaccharides content was small, at around 30%. Basic nutrients and inorganic elements from the soil for comparison, Pseudostellaria heterophylla from different habitats and cultivation of soil nutrients and inorganic elements contents were very uneven. The contents of Pb, Cu and B in the soil sample of Guizhou Province were the highest, and Cr and available phosphorus content in the soil sample of Shandong Province, Zn and effective potassium in the soil sample of Fujian Province all were the highest. With reference to the Soil Environment Quality Standard (GB15618-1995), most of Pseudostellaria heterophylla soil reached the national standard. From the point of soil elements and medicinal materials quality correlation, pseudostellarin B content and polysaccharide content had no significant correlation between each element in the soil. CONCLUSION: The ammonium nitrogen, effective potassium, available phosphorus and elements of Pb, Cr, Cu, Zn and B in the soil have no direct effect on effective component content of medicinal materials. 60% of Pseudostellaria heterophylla origin is generally lack of B in soil. It should be appropriate to increase the percentage of boron in the fertilizer management to ensure the quality of Pseudostellaria heterophylla root.


Assuntos
Caryophyllaceae/química , Raízes de Plantas/química , Solo/química , Ecossistema , Fertilizantes , Nitrogênio , Peptídeos Cíclicos/análise , Fósforo , Plantas Medicinais/química , Polissacarídeos/análise , Potássio , Controle de Qualidade
17.
Zhong Yao Cai ; 38(4): 730-4, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26672337

RESUMO

OBJECTIVE: To screen specific DNA sequence by rDNA-ITS sequence analysis at Eucommmia Cortex and its adulterants, then specific primers were designed to identify Eucommmia Cortex rapidly. METHODS: rDNA-ITS sequences of Eucommmia Cortex and its adulterants were downloaded from NCBI. The specific DNA sequence in the rDNA-ITS sequence of Eucommmia Cortex was found by compared with all sequences. Specific primers for authentic the Eucommmia Cortex were designed. PCR amplification and detection methods were optimized. RESULTS: PCR amplification products were detected through agarose gel electrophoresis and fluorescence reaction. The 400 bp identification band for Eucommmia Cortex could be produced and green fluorescent could be observed under ultraviolet light after adding the dye SYBR Green I. However its adulterants were without identification hand and green fluorescent. CONCLUSION: Specific PCR molecular identification can be applied to identify Eucommmia Cortex, the time is shortened significantly by PCR amplification optimization, and fluorescence detection can be more visually display the identification results. In a word, this study provides new ideas and methods for rapid identification of Eucommmia Cortex.


Assuntos
Medicamentos de Ervas Chinesas , Eucommiaceae/genética , Reação em Cadeia da Polimerase , Corantes , Primers do DNA , DNA de Plantas/genética , Eletroforese em Gel de Ágar , Fluorescência , Piperidinas , Casca de Planta , Tioxantenos
18.
Zhongguo Zhong Yao Za Zhi ; 40(18): 3573-8, 2015 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-26983202

RESUMO

To establish a molecular identification method for Bletillae Rhizoma, this paper extracted genome DNA from Bletillae Rhizoma and its adulterants. The sequences of rDNA ITS2 were sequenced after amplifying. Then multiple alignments of ITS2 were constructed phylogenetic tree with Neighbor Joining by MEGA 5. 1 and found out SNPs loci. The result showed that rDNA ITS2 region could identify Bletillae Rhizoma and its adulterants. There existed the SNPs loci, which could identify Bletilla striata and B. ochracea. Furthermore, we designed specific primers against the SNPs loci of B. striata and B. ochracea, then screened primers and optimized the PCR amplification conditions. Finally, the DNA of B. striata and B. ochracea were specifically amplified by BJ59-412F, BJ59-412R and HHBJ-225R. The length of amplification products were respectively about 350 bp and 520 bp that were effectively identified of B. striata and B. ochracea. While, the adulterants of Bletillae Rhizoma were no-reaction occurring. To sum up, the amplification conditions of the primers can identify B. striata, B. ochracea and their adulterants successfully at the same time. This method was easy, time-saving, and reliable, which can be used as a rapid method for molecular identification of Bletillae Rhizoma.


Assuntos
DNA Intergênico/genética , DNA de Plantas/genética , Orchidaceae/genética , Polimorfismo de Nucleotídeo Único , Sequência de Bases , Primers do DNA/genética , Contaminação de Medicamentos/prevenção & controle , Dados de Sequência Molecular , Orchidaceae/classificação , Filogenia , Rizoma/classificação , Rizoma/genética
19.
Zhong Yao Cai ; 38(8): 1600-6, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26983228

RESUMO

OBJECTIVE: To study the phenotypic genetic diversity of cultivated Pseudostellaria heterophylla, in order to provide theoretical basis for creative utilization and breeding of Pseudostellaria heterophylla germplasm resources. METHODS: The phenotypic genetic diversity of 80 germplasm of Pseudostellaria heterophylla from two superior provenance was analyzed, and the phenotypic characteristics among germplasm and within population were researched by correlation analysis, principal component analysis and cluster analysis. RESULTS: There were abundant phenotypic genetic diversity of Pseudostellaria heterophylla in two cultivated provenance. The genetic diversity index ranged from 1.2446 to 2.0560 and variation coefficient ranged from 13.22% to 59.24%. The leaf dry weight and leaf number had significant correlation (P < 0.05 or P < 0.01) with traits among root tuber. Six main principal components reflected most information of 23 phenotypic traits, and the accumulative contribution rate was 76.47%. The result of the cluster analysis was that 80 germplasm of Pseudostellaria heterophylla could be divided into four groups based on phenotypic difference, the first group can be parents of progenies with high yield potential, the second group could be optimal parents of progenies with high yield, the third group can be parents of first class goods. CONCLUSION: The germplasm resources of cultivated Pseudostellaria heterophylla from Guizhou is useful for breeding of new varieties with abundant diversity. The second group and the third group of Pseudostellaria heterophylla have better germplasm,which can be provide parents for cross breeding of Pseudostellaria heterophylla.


Assuntos
Caryophyllaceae/genética , Variação Genética , Fenótipo , Plantas Medicinais/genética , Cruzamento , Folhas de Planta , Tubérculos
20.
Zhongguo Zhong Yao Za Zhi ; 39(16): 3042-7, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25509284

RESUMO

Referring to the rules for agricultural seed testing (GB /T 3543-1995) issued by China, the test of sampling, seed purity, weight per 1 000 seeds, seed moisture, seed viability and germination rate had been studied for screening seed quality test methods of Pesudostellaria heterophylla. The seed quality from different collection areas was measured. The results showed that at least 6.5 g seeds should be sampled and passed through 10-mesh sieve for purity analysis. The weight of 1 000 seeds was determined by using the 500-seed method. The phenotypic observation and size measurement were used for authenticity testing. The seed moisture was determined under the higher temperature (130 ± 2) degrees C for 5 hours. The seeds were dipped into 0.2% TTC sustaining 1 hour at 40 degrees C, then the viability could be determined. The break dormancy seeds were cultured on sand at 10 degrees C. K cluster analysis was applied for the data analysis, the seed quality from different collection areas grading of P. Heterophylla was described as three grades. The seed quality of each grade should reach following requirements: for first grade seeds, germination rate ≥ 86%, 1 000-grain weight ≥ 2.59 g, purity ≥ 87%, moisture ≤ 13.1%; for second grade seeds, germination rate ≥ 70%, 1 000-grain weight ≥ 2.40 g, purity ≥ 77%, moisture ≤ 14.3%; for third grade seeds, germination rate ≥ 41%, 1 000-grain weight ≥ 2.29 g, purity ≥ 76%, moisture ≤ 15.8%. The seed testing methods for quality items of P. heterophylla had been initially established, as well as the primary P. heterophylla seed quality classification standard.


Assuntos
Caryophyllaceae/química , Sementes/química , Caryophyllaceae/crescimento & desenvolvimento , Germinação , Controle de Qualidade , Sementes/crescimento & desenvolvimento , Água/análise
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