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1.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2459-2466, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359711

RESUMO

This paper is aimed to study the commodity specification and grade standard of Galla Chinensis,for standardizing market order and guide the market circulation,and provide a basis for standardization of Galla Chinensis. With 33 samples of Galla Chinensis from market as the object of the research,the herbal textual research and market research were carried out. Then the grading indicator were selected by the descriptive statistics,principal component analysis and cluster analysis,combining with production practice,the commodity specification and grade standard of Galla Chinensis were set out. The data of moisture,ash,gallic acid as the internal index were used to analyze the relationship between the quality difference between grades and the appearance characters and the intrinsic composition. Herbal textual research and market research found that the high quality of Galla Chinensis characterized with large,complete,wall thick,grayish brown characteristics,and Galla Chinensis could be divided into gallnuts and horned gall. Through principal component analysis and cluster analysis,combining actual production and herbal record,screening,the length,diameter,single weight,the number of 500 g were determined as 4 grading indicators,the commodity specification was divided into two: gallnuts and horned gall,the grades was divided into two: selected goods and mixed goods. The result of correlation analysis showed there was no significant correlation between the internal index and the appearance characters of Galla Chinensis. The result of multiple comparison showed that the ash content of the selected goods was smaller than that of the mixed goods,but it did not reach significant. The content of gallic acid of the selected goods and the mixed goods of gallnuts were higher than selected goods of horned gall,and higher than mixed goods of horned galls. Using the length,diameter,single weight,the number of 500 g as the appearance characters index. Combining with the herbal textual research and market research,we have divided two specifications and two grades for the commodity specification and grade standard of Galla Chinensis,which can provide a basis for industry standards and national standards.


Assuntos
Medicamentos de Ervas Chinesas/normas , Ácido Gálico/análise , Tumores de Planta , Controle de Qualidade
2.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2219-2225, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359645

RESUMO

The study aims at investigating the grade standard and the quality characteristic of Pinelliae Rhizoma for commodity specification, which provides the reference for its grade standard formulation. 42 Pinelliae Rhizoma simples were collected from 5 medicinal materials markets and 2 producing areas. Based on the previous herbalogical study and market investigation, we combined with the data analysis to select the grading indicators using SPSS software for descriptive statistical analysis, analysis of variance, K-cluster analysis and correlation analysis. According to the actual production condition, we developed the grading standards of Pinelliae Rhizoma. Moreover, we compared the internal indicators(water, total ash, leachate and guanosine) of Pinelliae Rhizoma at various grade levels, and analyzed the correlation between appearance traits and internal indicators. The herbalogical study and market research found that the Pinelliae Rhizoma was better in large, solid and white. The results from descriptive and variance analysis showed that the appearance of Pinelliae Rhizoma was significantly different in weight per grain and grain number of 500 g. Referring to the 2015 Chinese Pharmacopoeia and the production practice, we use the length, weight per grain and grain number of 500 g as the classification index of Pinelliae Rhizoma. The results from correlation analysis showed that there was no significant correlation between the appearance of Pinelliae Rhizoma and the intrinsic quality index. In addition, we found there was no significant difference in the content of the intrinsic index except for the total ash and the extract. The current study established the classification index of the product specification and grade standard of Pinelliae Rhizoma with length, weight per grain and grain number of 500 g as the index, which can provide the basis for the classification of the product specification and grade of Pinelliae Rhizoma market.


Assuntos
Medicamentos de Ervas Chinesas/normas , Pinellia/química , Rizoma/química
3.
Zhongguo Zhong Yao Za Zhi ; 44(2): 265-269, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989943

RESUMO

This study is based on the data analysis of medicinal plant resources and diversity collected from the fourth Chinese traditional medicine resource survey( pilot). Through the analysis of relevant data from 33 census pioneer plots in Guizhou province( area),a total of 265 families,1 432 genera and 5 296 species of medicinal resources were reported,including algae,fungi,lichens,mosses,a total of 43 genera and 35 families,57,48 families,120 genera and 453 species of ferns,gymnosperms 11 families,22 genera and 61 species,167 families,1 243 genera and 4 721 species of angiosperms,4 genera and 4 families four medicinal animals.Compared with the data related to the third survey of traditional Chinese medicine resources,the number of ferns,gymnosperms and angiosperms in the fourth survey has increased far more than that of the third survey. From the regional distribution of medicinal resources,the composition of the genus,the type of life,and the location of the medicine,the richness of the medicinal plant resources in Guizhou province is not only reflected in many types,but also in the variety of medicinal resources. These studies provide a scientific basis for vigorously developing the Chinese herbal medicine industry and the sustainably using medicinal plant resources in Guizhou province.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Plantas Medicinais/classificação , China , Cycadopsida , Gleiquênias , Magnoliopsida
4.
Zhongguo Zhong Yao Za Zhi ; 43(21): 4210-4216, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30583619

RESUMO

The study aims at evaluating genetic diversity and medicinal quality of cultivated germplasm in Rehmannia glutinosa, and providing theoretical guidance for screening excellent germplasm. The genetic diversity of 21 species of R. glutinosa were analyzed by SRAP molecular markers, and the catalpol and verbascoside was determined by HPLC. The mass fraction of catalpol and verbascoside in R. glutinosa germplasm were respectively in the range of 2.393%-6.519% and 0.063%-0.478%, the germplasm 14, 16, 15 and 20 germplasm, witch catalpol and verbascoside content was higher. A total of 57 bands were produced by 10 primer, among which 40 polymorphic bands were polymorphic bands, and the percentage of polymorphic loci was 8.77%-54.39%, the Nei's genetic diversity index (H) was 0.374 1, Shannon's polymorphism information index (I) was 0.546 6. Gst and gene flow Nm were 0.608 8 and 0.321 3, respectively. Based on the genetic uniformity, 21 species of germplasm were grouped into 2 categories. The genetic diversity level of R. glutinosa was medium low. The comprehensive consideration of the genetic diversity and the content inculde catalpol and verbascoside, germplasm 7 and germplasm 18 could be used as the preferred materials for the cultivation of reticulum. Germplasm 15 and 16 can be used as the preservation and breeding object of rhubarb germplasm.


Assuntos
Variação Genética , Rehmannia/genética , Animais , Fluxo Gênico , Filogenia , Melhoramento Vegetal , Plantas Medicinais/genética
5.
Zhongguo Zhong Yao Za Zhi ; 42(5): 882-889, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994530

RESUMO

To provide guidance for certification, popularization and application of Pseudostellariae Radix new variety, the regional adaptation and stabilities of "Shitai No.1" were evaluated. The "Qian taizishen No.1" and "SB-C" varieties (strains) were used as the control varieties. The agronomic, medicinal material traits and medicine quality were used as evaluation index to compare the phenotypic difference of the three varieties (strains) in four planting areas. Compared to the control varieties, 10 agronomic traits of "Shitai No.1" had the smallest coefficient of variation among the 18 agronomic traits, and other 8 agronomic traits placed the middle level. Among 8 medicinal material traits and medicine quality indicators, the coefficient of variation of different regions of the extract content, pseudostellarin B content, the number of 50 g root tuber, the plant medicinal materials weight and weight of single root of "Shitai No.1" were the smallest compare to other varieties (strains). It could be divided into three groups based on the phenotypic difference of the three varieties (strains) in four planting areas. The "Shitai No.1" was classified as one group, while the "Qian taizishen No.1" and "SB-C" had cross clustering. The regional stability of several index about agronomic traits, medicinal material traits and medicine quality of "Shitai No.1" were better than that of the control varieties (strains). "Shitai No.1" was suitable for planting, popularization and application in the appropriate ecological areas of Guizhou province.


Assuntos
Caryophyllaceae/química , Medicamentos de Ervas Chinesas/normas , Raízes de Plantas/química , China , Geografia , Peptídeos Cíclicos/análise , Fenótipo , Tubérculos/química , Plantas Medicinais/química
6.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2381-2385, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905556

RESUMO

Based on collections and researches of Pesudostellaria heterophylla germplasm resources from different areas of China, by using Shibing SB-4 provenance as materials, the new variety "Shitai No.1" was bred by mass selection, small plot variety comparative test, regional variety comparative test and field trial planting. Compared with "Qian taizishen No.1" and P. heterophylla land races. The disease and lodging resistance, root yield, polysaccharide content and the first grade rate of "Shitai No.1" have obvious advantages. In addition, it is relatively stable of yield in "Shitai No.1" in different places. It is demonstrated that "Shitai No.1" is a fine variety that adapt to the producing areas of P. heterophylla in Guizhou province, it is worthy to be promoted.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Melhoramento Vegetal , China , Raízes de Plantas , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise
7.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2386-2390, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905557

RESUMO

Maxent model was applied in the study to filtering the climate factors layer by layer. Polysaccharides and pseudostellarin B the two internal quality evaluation index were combined to analyse the interlinkages between climate factors and chemical constituents in order to search for the critical climate factors of Pseudostellaria heterophylla. Then based on the key climate factors to explicit the quality spatial distribution of P. heterophylla. The results showed that polysaccharides and climatic factors had no significant correlation, suggesting that the indicator was not climate-driven metabolites. Pseudostellarin B could construct regression model with the precipitation. And quality regionalization results showed that pseudostellarin B content presented firstly increased and then decreased trend from southeast to northwest, which was the consistent change with precipitation. It clearly proposed that precipitation was the key climate factor, which affected the accumulation of cyclopeptide compound for Pseudostellariae Radix.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Clima , Peptídeos Cíclicos/análise , Raízes de Plantas , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise , Pesquisa
8.
Zhongguo Zhong Yao Za Zhi ; 41(13): 2391-2396, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28905558

RESUMO

This paper is aimed to study the potential ecological suitability regionalization of Pseudostellaria heterophylla for selecting GAP planting base location and designing rational production layout. The ecological factors and contribution rate were determined by using maximum entropy (Maxent) model. Then, the information entropy theory was used to determine the relative importance of each environmental factor, and thus to determine the most limiting habitat criteria. Finally, the probable spatial distribution of P. heterophylla was determined based on GIS spatial analysis of habitat conditions. Meanwhile, the optimal index range of ecological factors was quantified. The moderately and highly suitable habitats were mainly located in Shibing, Huangping, Cengong, the middle and east of Kaili, the south of Yuqing, the west of Tongren. The percentage of moderately and highly suitable habitats for P. heterophylla in the study area was 3.64%, and its area was 6 405.39 km². The results also showed that seven dominant ecological factors controlled the distribution of P. heterophylla. These factors included agrotype, the warmest rain, aspect, slope, the warmest and highest temperature, contents of soil organic carbon, and the driest month precipitation. The habitat suitability assessment model based on GIS and Maxent model theory could accurately evaluate the habitat suitability distribution of P. heterophylla in Guizhou. In addition, we recommended Cengong and Zhenyuan county in Guizhou province as the worthy developing potential planting areas.


Assuntos
Caryophyllaceae/crescimento & desenvolvimento , Ecossistema , China , Ecologia , Entropia , Plantas Medicinais/crescimento & desenvolvimento , Controle de Qualidade , Solo
9.
Zhong Yao Cai ; 38(4): 674-8, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26672330

RESUMO

OBJECTIVE: To study the effect of basic soil nutrients and inorganic elements on the quality of Pseudostellaria heterophylla root, in order to reveal the inner link and to provide the scientific basis for rational cultivation of Pseudostellaria heterophylla. METHODS: The medicinal materials of Pseudostellariae Radix and soil samples from 15 habitats were collected, and three essential nutrients, five inorganic elements in the soil and the medicinal contents of polysaccharides and pseudostellarin B were determined. Then using SPSS software to analyze its relevance. RESULTS: Significant difference of pseudostellarin B content was found in samples from different provinces, which was not detected in the sample of Fujian Province, but the difference of polysaccharides content was small, at around 30%. Basic nutrients and inorganic elements from the soil for comparison, Pseudostellaria heterophylla from different habitats and cultivation of soil nutrients and inorganic elements contents were very uneven. The contents of Pb, Cu and B in the soil sample of Guizhou Province were the highest, and Cr and available phosphorus content in the soil sample of Shandong Province, Zn and effective potassium in the soil sample of Fujian Province all were the highest. With reference to the Soil Environment Quality Standard (GB15618-1995), most of Pseudostellaria heterophylla soil reached the national standard. From the point of soil elements and medicinal materials quality correlation, pseudostellarin B content and polysaccharide content had no significant correlation between each element in the soil. CONCLUSION: The ammonium nitrogen, effective potassium, available phosphorus and elements of Pb, Cr, Cu, Zn and B in the soil have no direct effect on effective component content of medicinal materials. 60% of Pseudostellaria heterophylla origin is generally lack of B in soil. It should be appropriate to increase the percentage of boron in the fertilizer management to ensure the quality of Pseudostellaria heterophylla root.


Assuntos
Caryophyllaceae/química , Raízes de Plantas/química , Solo/química , Ecossistema , Fertilizantes , Nitrogênio , Peptídeos Cíclicos/análise , Fósforo , Plantas Medicinais/química , Polissacarídeos/análise , Potássio , Controle de Qualidade
10.
Zhong Yao Cai ; 38(4): 730-4, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26672337

RESUMO

OBJECTIVE: To screen specific DNA sequence by rDNA-ITS sequence analysis at Eucommmia Cortex and its adulterants, then specific primers were designed to identify Eucommmia Cortex rapidly. METHODS: rDNA-ITS sequences of Eucommmia Cortex and its adulterants were downloaded from NCBI. The specific DNA sequence in the rDNA-ITS sequence of Eucommmia Cortex was found by compared with all sequences. Specific primers for authentic the Eucommmia Cortex were designed. PCR amplification and detection methods were optimized. RESULTS: PCR amplification products were detected through agarose gel electrophoresis and fluorescence reaction. The 400 bp identification band for Eucommmia Cortex could be produced and green fluorescent could be observed under ultraviolet light after adding the dye SYBR Green I. However its adulterants were without identification hand and green fluorescent. CONCLUSION: Specific PCR molecular identification can be applied to identify Eucommmia Cortex, the time is shortened significantly by PCR amplification optimization, and fluorescence detection can be more visually display the identification results. In a word, this study provides new ideas and methods for rapid identification of Eucommmia Cortex.


Assuntos
Medicamentos de Ervas Chinesas , Eucommiaceae/genética , Reação em Cadeia da Polimerase , Corantes , Primers do DNA , DNA de Plantas/genética , Eletroforese em Gel de Ágar , Fluorescência , Piperidinas , Casca de Planta , Tioxantenos
11.
Zhongguo Zhong Yao Za Zhi ; 40(18): 3573-8, 2015 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-26983202

RESUMO

To establish a molecular identification method for Bletillae Rhizoma, this paper extracted genome DNA from Bletillae Rhizoma and its adulterants. The sequences of rDNA ITS2 were sequenced after amplifying. Then multiple alignments of ITS2 were constructed phylogenetic tree with Neighbor Joining by MEGA 5. 1 and found out SNPs loci. The result showed that rDNA ITS2 region could identify Bletillae Rhizoma and its adulterants. There existed the SNPs loci, which could identify Bletilla striata and B. ochracea. Furthermore, we designed specific primers against the SNPs loci of B. striata and B. ochracea, then screened primers and optimized the PCR amplification conditions. Finally, the DNA of B. striata and B. ochracea were specifically amplified by BJ59-412F, BJ59-412R and HHBJ-225R. The length of amplification products were respectively about 350 bp and 520 bp that were effectively identified of B. striata and B. ochracea. While, the adulterants of Bletillae Rhizoma were no-reaction occurring. To sum up, the amplification conditions of the primers can identify B. striata, B. ochracea and their adulterants successfully at the same time. This method was easy, time-saving, and reliable, which can be used as a rapid method for molecular identification of Bletillae Rhizoma.


Assuntos
DNA Intergênico/genética , DNA de Plantas/genética , Orchidaceae/genética , Polimorfismo de Nucleotídeo Único , Sequência de Bases , Primers do DNA/genética , Contaminação de Medicamentos/prevenção & controle , Dados de Sequência Molecular , Orchidaceae/classificação , Filogenia , Rizoma/classificação , Rizoma/genética
12.
Zhong Yao Cai ; 38(8): 1600-6, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26983228

RESUMO

OBJECTIVE: To study the phenotypic genetic diversity of cultivated Pseudostellaria heterophylla, in order to provide theoretical basis for creative utilization and breeding of Pseudostellaria heterophylla germplasm resources. METHODS: The phenotypic genetic diversity of 80 germplasm of Pseudostellaria heterophylla from two superior provenance was analyzed, and the phenotypic characteristics among germplasm and within population were researched by correlation analysis, principal component analysis and cluster analysis. RESULTS: There were abundant phenotypic genetic diversity of Pseudostellaria heterophylla in two cultivated provenance. The genetic diversity index ranged from 1.2446 to 2.0560 and variation coefficient ranged from 13.22% to 59.24%. The leaf dry weight and leaf number had significant correlation (P < 0.05 or P < 0.01) with traits among root tuber. Six main principal components reflected most information of 23 phenotypic traits, and the accumulative contribution rate was 76.47%. The result of the cluster analysis was that 80 germplasm of Pseudostellaria heterophylla could be divided into four groups based on phenotypic difference, the first group can be parents of progenies with high yield potential, the second group could be optimal parents of progenies with high yield, the third group can be parents of first class goods. CONCLUSION: The germplasm resources of cultivated Pseudostellaria heterophylla from Guizhou is useful for breeding of new varieties with abundant diversity. The second group and the third group of Pseudostellaria heterophylla have better germplasm,which can be provide parents for cross breeding of Pseudostellaria heterophylla.


Assuntos
Caryophyllaceae/genética , Variação Genética , Fenótipo , Plantas Medicinais/genética , Cruzamento , Folhas de Planta , Tubérculos
13.
Zhongguo Zhong Yao Za Zhi ; 39(16): 3042-7, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25509284

RESUMO

Referring to the rules for agricultural seed testing (GB /T 3543-1995) issued by China, the test of sampling, seed purity, weight per 1 000 seeds, seed moisture, seed viability and germination rate had been studied for screening seed quality test methods of Pesudostellaria heterophylla. The seed quality from different collection areas was measured. The results showed that at least 6.5 g seeds should be sampled and passed through 10-mesh sieve for purity analysis. The weight of 1 000 seeds was determined by using the 500-seed method. The phenotypic observation and size measurement were used for authenticity testing. The seed moisture was determined under the higher temperature (130 ± 2) degrees C for 5 hours. The seeds were dipped into 0.2% TTC sustaining 1 hour at 40 degrees C, then the viability could be determined. The break dormancy seeds were cultured on sand at 10 degrees C. K cluster analysis was applied for the data analysis, the seed quality from different collection areas grading of P. Heterophylla was described as three grades. The seed quality of each grade should reach following requirements: for first grade seeds, germination rate ≥ 86%, 1 000-grain weight ≥ 2.59 g, purity ≥ 87%, moisture ≤ 13.1%; for second grade seeds, germination rate ≥ 70%, 1 000-grain weight ≥ 2.40 g, purity ≥ 77%, moisture ≤ 14.3%; for third grade seeds, germination rate ≥ 41%, 1 000-grain weight ≥ 2.29 g, purity ≥ 76%, moisture ≤ 15.8%. The seed testing methods for quality items of P. heterophylla had been initially established, as well as the primary P. heterophylla seed quality classification standard.


Assuntos
Caryophyllaceae/química , Sementes/química , Caryophyllaceae/crescimento & desenvolvimento , Germinação , Controle de Qualidade , Sementes/crescimento & desenvolvimento , Água/análise
14.
Zhongguo Zhong Yao Za Zhi ; 39(15): 2873-80, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25423825

RESUMO

OBJECTIVE: To study the commodity specification and grade standard of Pseudostellaria Radix, for standardizing market order and achieving industrialization, standardization and modernization of Pseudostellariae Radix. METHOD: The different areas and grade medicinal materials of Pseudostellariae Radix were respectively measured in quantitative characteristics of appearance, and the present grade classification of Pseudostellaria Radix was scientifically verified by the content of polysaccharide. Then the determination data were evaluated by spss correlation analysis, principal component analysis and cluster analysis. So combining with the actual production, the commodity grade standard of Pseudostellariae Radix was formulated. RESULT: Correlation analysis indicated that the present grade classification of Pseudostellaria Radix was reasonable, and the more the grade of Pseudostellariae Radix was high, the more the content of polysaccharide was high. Meanwhile, length as a classification index was not suitable for the commodity grade standard of Pseudostellariae Radix. Using principal component analysis and cluster analysis, combining actual production, the thickest diameter, weight of single root tuber and the number of 50 g root tuber were filtrated and the grade was divided into 5 ranks: big, mid- dle, small selected goods, big ungraded goods and small ungraded goods. CONCLUSION: the commodity specification and grade standard of Pseudostellariae Radix that mainly included the thickest diameter, weight of single root tuber and the number of 50 g root tuber was formulated, the standard was divided into 5 grade. Each grade was not only consistent with the present situation of medicinal materials market, it could also reflected the intrinsic quality of Pseudostellariae Radix. In conclusion, the standard could be used as a classifica- tion basis to the commodity specification and grade.


Assuntos
Caryophyllaceae/química , Medicamentos de Ervas Chinesas/normas , Medicamentos de Ervas Chinesas/química , Controle de Qualidade , Padrões de Referência
15.
Zhong Yao Cai ; 37(5): 760-6, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25335280

RESUMO

OBJECTIVE: To study the ecological suitability regionalization of Eucommia ulmoides, for selecting artificial planting base and high-quality industrial raw material purchase area of the herb in Guizhou. METHODS: Based on the investigation of 14 Eucommia ulmoides producing areas, pinoresinol diglucoside content and ecological factors were obtained. Using spatial analysis method to carry on ecological suitability regionalization. Meanwhile, combining pinoresinol diglucoside content, the correlation of major active components and environmental factors were analyzed by statistical analysis. RESULTS: The most suitability planting area of Eucommia ulmoides was the northwest of Guizhou. The distribution of Eucommia ulmoides was mainly affected by the type and pH value of soil, and monthly precipitation. The spatial structure of major active components in Eucommia ulmoides were randomly distributed in global space, but had only one aggregation point which had a high positive correlation in local space. The major active components of Eucommia ulmoides had no correlation with altitude, longitude or latitude. CONCLUSION: Using the spatial analysis method and statistical analysis method, based on environmental factor and pinoresinol diglucoside content, the ecological suitability regionalization of Eucommia ulmoides can provide reference for the selection of suitable planting area, artificial planting base and directing production layout.


Assuntos
Ecologia , Eucommiaceae/crescimento & desenvolvimento , Geografia Médica , Plantas Medicinais/crescimento & desenvolvimento , Adaptação Biológica , China , Clima , Eucommiaceae/química , Eucommiaceae/fisiologia , Sistemas de Informação Geográfica , Lignanas/análise , Modelos Teóricos , Casca de Planta/química , Casca de Planta/crescimento & desenvolvimento , Plantas Medicinais/química , Plantas Medicinais/fisiologia , Solo
16.
Zhongguo Zhong Yao Za Zhi ; 39(19): 3689-94, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25612422

RESUMO

To establish a convenient and rapid method for identification of Pseudostellariae Radix by molecular identification, the rDNA-ITS sequences of Pseudostella riaheterophylla and its adulterants had been aligned to find out specific fragment. The specific primers against the fragment were designed and the PCR amplification conditions were optimized. The fluorescence reaction of the PCR products colored by 100 x SYBR Green I was observed under UV. The concentration of reaction buffer included 5.5 µL DNA Taq polymerase premix, 10 pmol Tzs-2F and 10 pmol Tzs-2R, 20-80 ng template DNA, and plus double sterile distilled water to 25 µL. The PCR thermal profile was as follows: predenaturation at 95 degrees C for 1 min, followed by 30 cycles of denaturation at 95 degrees C for 5 seconds, primer annealing and extension at 56 degrees C for 15 seconds, then it was extension at 72 degrees C for 30 seconds. The fluorescence reaction of Pseudostellariae Radix showed green fluorescence, while adulterants had not. Extraction, amplification DNA and all steps of molecular identification could be completed successfully in 40 minutes. The approach could amplify DNA template of Pseudostellariae Radix specificity, and its product with 1 µL 100 x SYBR Green I could engender green fluorescence under UV. The method was simple and accurate, so it could be used for identification of Chinese traditional medicine.


Assuntos
Caryophyllaceae/genética , Reação em Cadeia da Polimerase/métodos , Caryophyllaceae/classificação , Primers do DNA/genética , Contaminação de Medicamentos/prevenção & controle , Raízes de Plantas/classificação , Raízes de Plantas/genética
17.
Zhongguo Zhong Yao Za Zhi ; 39(21): 4197-204, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25775793

RESUMO

OBJECTIVE: To provide new germplasm materials for breeding new varieties of Pseudostellaria heterophylla. METHOD: The method of single plant selection was adopted, with the comparative experiments being carried out under the same conditions in Shibing county. The 8 plants of Shibing SB-4 were compared respectively with factor analysis for 27 phenotypic traits and 8 yield traits, and single factor variance analysis for the contents of polysaccharides. RESULT: Using factor analysis, 27 phenotypic traits were classified into 7 principal divisors and 8 yield traits were simplified into 3 principal divisors. The 4 strains of P. heterophylla, ZT-01, ZT-02, ZT-06 and ZT-07, performed better than others in the phenotypic traits, and ZT-01, ZT-02, ZT-03 and ZT-07 in the yield traits. The contents of polysaccharides of ZT-01, ZT-02, ZT-05 and ZT-08 showed significantly higher value. CONCLUSION: There is significant difference among the 8 strains of P. heterophylla in phenotypic traits, yield traits and quality traits, making it possible to select certain strains for different purposes. ZT-01 and ZT-02 can be breaded further. ZT-06 and ZT-07 were used as ornamental cultivars for its great phenotypic traits. ZT-03 with good resistance and high yield was taken as resistant variety, and ZT-05 would face next selection on the basis of its high content of polysaccharide.


Assuntos
Cruzamento , Caryophyllaceae/crescimento & desenvolvimento , Caryophyllaceae/química , China , Fenótipo , Polissacarídeos/análise
18.
Zhong Yao Cai ; 37(8): 1343-9, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25726639

RESUMO

OBJECTIVE: To explore the phenotypic characteristics and the genetic diversity of cultivated Eucommia ulmoides from Guizhou Province. METHODS: The genetic diversity of cultivated Eucommia ulmoides in Guizhou Province was analyzed by phenotypic traits and start condon targeted polymorphism(SCoT)markers. RESULTS: The phenotypic diversity index of the number of buds on neonatal branch was the highest, which was 2.0638, and the number of blades on neonatal branch was the lowest,which was 1.7084. By data obtained, 40 cultivated samples of Eucommia ulmoides in Guizhou Province could be divided into 4 groups. A total of 76 bands were produced by 10 primers, among which 50 bands were polymorphic bands, and the percentage of polymorphic bands was 65.79%. The average value of Nei's genetic diversity index (H) was 0.1937, Shannon's information index (I) was 0.2832, Genetic differentiation coefficient (Gst) was 0.1733, and the gene flow (Nm) among populations was 2.3848. Cluster analysis based on genetic identity indicated that 4 populations could be divided into 2 groups. CONCLUSION: Genetic diversity of cultivated populations of Eucommia ulmoides in Guizhou Province had little differences. The genetic differentiation within populations was bigger than that among the populations. The high level of gene flow among populations was beneficial to extension of Eucommia ulmoides. The phenotypic cluster was similar to the SCoT cluster, the two classification results were significantly correlated with geographic position,which can providing a good foundation for the identification of Eucommia ulmoides germplasms.


Assuntos
Eucommiaceae/genética , Marcadores Genéticos , Análise por Conglomerados , Primers do DNA , Fluxo Gênico , Fenótipo , Polimorfismo Genético
19.
Zhongguo Zhong Yao Za Zhi ; 38(6): 812-6, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23717957

RESUMO

OBJECTIVE: To provide guidance for selection and breeding of Pesudostellaria heterophylla, agronomic traits of 3 mainly cultivated form of P. heterophylla were observed and compared in Guizhou shibing. METHOD: Thirteen agronomic traits of P. heterophylla from 3 cultivation form were measured and the traits were analyzed using multiple comparison, correlation analysis, multiple stepwise regression analysis and path analysis. RESULT: The results showed that agronomic traits were different in 3 cultivation form, significant(P <0.05)or very significant correlated (P <0.01)among multiple agronomic traits, the width and the number of root, the first branch number, cleistogamous flowers, length and width of leaves, and the aboveground biomass were the main factors that affected the underground biomass, and the number of root, the aboveground biomass had a directly positive effect on the underground biomass. Meanwhile, whole length, length and width of leaves, cleistogamous flowers etc. had direct or indirect effect on the underground biomass. CONCLUSION: Aboveground biomass would be as the best indirect selection traits on breeding high yield of P. heterophylla, the first branch number, width of leaves and cleistogamous flowers world be as a better auxiliary index on breeding high variety of P. heterophylla.


Assuntos
Agricultura/economia , Hemípteros/crescimento & desenvolvimento , Animais , China , Hemípteros/anatomia & histologia , Análise de Regressão
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