Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 542
Filtrar
1.
Food Chem ; 338: 128115, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33092006

RESUMO

Citrus flavonoids consist of diverse analogs and possess various health-promoting effects dramatically depending on their chemical structures. Since different flavonoids usually co-exist in real samples, it's necessary to develop rapid and efficient methods for simultaneous determination of multiple flavonoids. Thin layer chromatography combined with surface enhanced Raman spectroscopy (TLC-SERS) was established to simultaneously separate and detect 14 citrus flavonoids for the first time. These target compounds could be characterized and discriminated when paired with SERS at 6-500 times greater the sensitivity than TLC alone. TLC-SERS exhibited high recovery rates (91.5-121.7%) with relative standard deviation lower than 20.8%. Moreover, the established TLC-SERS method was successfully used to simultaneously detect multiple flavonoids in real samples, which exhibited comparable accuracy to high performance liquid chromatography with shorter analytical time (10 vs 45 min). All the results demonstrated that this could be a promising method for simultaneous, rapid, sensitive and accurate detection of flavonoids.

2.
J Food Sci ; 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33159467

RESUMO

Bakery products made by cereal and edible fungi powder have a unique flavor and health benefits, dramatically enhancing the nutritional value of the products. In this study, we investigated the antifatigue effect of a novel Tricholoma matsutake cookie (TMC) by the exhaustive swimming test. Male Kunming ICR mice were randomly divided into seven groups (each group, n = 10), fed with saline, ordinary cookies (4, 8, 16 g/kg B.W./day), and TMC (4, 8, 16 g/kg B.W./day) by gavage. After 30-day administration, the weight-loaded swimming test was carried out on the mice to evaluate the antifatigue effect of TMC. In comparison with the effect of ordinary cookies, the intake of TMC significantly prolonged the exhaustive swimming time of mice and increased the level of muscle glycogen and liver glycogen, accompanied by the reduction of lactic acid and urea nitrogen level in serum. Additionally, TMC dramatically improved the activity of superoxide dismutase and glutathione peroxidase in serum and largely decreased the level of malondialdehyde. All in all, TMC could enforce exhaustive swimming tolerance, accelerate the decomposition of sports-related metabolites such as lactic acid and urea nitrogen, and increase the activity of the antioxidant enzyme, thereby improving sports-related energy storage and relieving fatigue. Our findings broadened the application of T. matsutake in the processing of bakery products and provided the theoretical basis and technical support for the development of antifatigue products. PRACTICAL APPLICATION: In this study, we investigated the antifatigue effect of a novel Tricholoma matsutake cookie by the exhaustive swimming test. Collectively, the results of the present study suggested that the cookies fortified with T. matsutake could be considered as an antifatigue bakery product. Furthermore, our findings broadened the application of T. matsutake in the processing of bakery products and provided the theoretical basis and technical support for the development of antifatigue products.

3.
Food Chem ; : 128536, 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33189481

RESUMO

Shepherd's purse as a wild vegetable is getting more and more attention on health benefits. Water extract of shepherd's purse (WESP) was prepared and analyzed for the chemical constituents. The mice were fed high-fructose (HF) diet and treated with WESP at 50, 100 and 200 mg/kg·bw for 8 weeks. The HF-fed mice receiving WESP exhibited the inhibitions against abnormal weight gain, hepatic fat accumulation and lipid metabolic by down-regulating FAS and ACC expressions. WESP also significantly alleviated hyperglycemia, oxidative stress, and inflammatory response by regulating of NF-κB pathway. Moreover, WESP dose-dependently increased the acetic, propionic, and butyric acids levels in HF-fed mice. Furthermore, WESP significantly alleviated the HF-induced gut dysbiosis by reducing the ratio of Firmicutes to Bacteroidetes and increasing the abundance of potential beneficial bacteria. Our findings indicate that WESP may be an effective dietary supplement for preventing the liver damage.

4.
Pest Manag Sci ; 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33128494

RESUMO

BACKGROUND: 4-Hydroxyphenylpyruvate dioxygenase (HPPD) plays an important role in addressing the issue of plant protection research. This study sheds new light on the differences in molecular scaffold from commercialized HPPD inhibitors. RESULTS: The compounds A1-A18 and B1-B27 were synthesized for in vitro and greenhouse experiments. The greenhouse experiment data indicated that compounds B14 and B18 displayed excellent herbicidal activity, which was higher compared to that of mesotrione. In vitro testing indicated that the compounds were HPPD inhibitors. Moreover, molecular simulation results show that the compounds B14, B18, and mesotrione shared similar interplay with surrounding residues, which led to a perfect interaction with the active site of Arabidopsis thaliana HPPD. Based on crop selectivity results, compounds B14 and B18 were selected for maize studies (injury≤10%), indicating its potential for weed control in maize fields. CONCLUSION: These results showed that the pyrazole-benzofuran structure could be used as possible lead compounds for the development of HPPD inhibitors.

5.
J Environ Sci (China) ; 98: 62-70, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33097159

RESUMO

In recent decades, coastal ports have experienced rapid development and become an important economic and ecological hub in China. Atmospheric particle is a research hotspot in atmospheric environmental sciences in inland regions. However, few studies on the atmospheric particle were conducted in coastal port areas in China, which indeed suffers atmospheric particle pollution. Lack of the physicochemical characteristics of fine particles serves as an obstacle toward the accurate control for air pollution in the coastal port area in China. Here, a field observation was conducted in an important coastal port city in Yangtze River Delta from March 6 to March 19, 2019. The average PM2.5 concentration was 63.7 ± 27.8 µg/m3 and NO3-, SO42-, NH4+, and organic matter accounted for ~60% of PM2.5. Fe was the most abundant trace metal element and V as the ship emission indicator was detected. Transmission electron microscopy images showed that SK-rich, soot, Fe, SK-soot and SK-Fe were the major individual particles in the coastal port. V and soluble Fe were detected in sulfate coating of SK-Fe particles. We found that anthropogenic emissions, marine sea salt, and secondary atmosphere process were the major sources of fine particles. Backward trajectory analysis indicated that the dominant air masses were marine air mass, inland air mass from northern Zhejiang and inland-marine mixed air mass from Shandong and Shanghai during the sampling period. The findings can help us better understand the physicochemical properties of atmospheric fine particles in the coastal port of Eastern China.


Assuntos
Poluentes Atmosféricos , Rios , Poluentes Atmosféricos/análise , China , Cidades , Monitoramento Ambiental
6.
Food Funct ; 11(11): 9936-9946, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33103174

RESUMO

The oil droplets in commercial emulsified foods have dimensions that vary widely, from hundreds of nanometers to tens of micrometers. Previously, the size of the droplets in oil-in-water emulsions has been shown to impact their gastrointestinal behavior, which may influence their physiological effects. In this study, we analyzed the impact of oil droplet diameter (0.16, 1.1 and 8.2 µm) on lipid digestion and nutraceutical bioaccessibility using a widely used standardized gastrointestinal tract model: the INFOGEST method. The emulsions used consisted of corn oil droplets stabilized using a food-grade non-ionic surfactant (Tween 20), and the droplet size was controlled by preparing them with a microfluidizer (small), sonicator (medium), or high-shear blender (large). The surfactant-coated oil droplets were relatively resistant to size changes in the mouth and stomach, due to the strong surface activity and steric stabilization mechanism of the non-ionic surfactant used. As expected, the kinetics of lipid digestion were enhanced for smaller droplets because of their greater specific surface area. The degree of lipid digestion fell from 117% to 78% (p < 0.001) as the initial droplet diameter was raised from 0.16 to 8.2 µm. In addition, there was a reduction in ß-carotene bioaccessibility from 83 to 15% (p < 0.001) with increasing droplet diameter. This result was ascribed to several effects: (i) some carotenoids were trapped inside the undigested oil phase; (ii) fewer mixed micelles were produced to internalize the carotenoids; and, (iii) a fraction of the carotenoids crystallized and sedimented. Our results underline the critical importance of considering droplet size when developing emulsified foods loaded with carotenoids. The results obtained by the INFOGEST method are consistent with those found using other in vitro methods in earlier studies.

7.
Sci Total Environ ; 741: 140470, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32886967

RESUMO

Chemical compositions of non-refractory submicron aerosol (NR-PM1) were measured via an Aerodyne Aerosol Chemical Speciation Monitor at the coastal city Xiamen during the 2017 BRICS summit from August 10 to September 10. Mean hourly concentration of NR-PM1 was 13.55 ± 8.83 µg m-3 during the study period, decreasing from 18.83 µg m-3 before-BRICS to 13.02 µg m-3 in BRCIS I and 8.42 µg m-3 in BRICS II. Positive matrix factor analyses resolved four organic aerosols (OA): a hydrocarbon-like OA (HOA, 14.78%), a cooking-related OA (COA, 28.21%), a biomass burning OA (BBOA, 18.00%), and an oxygenated OA (OOA, 39.22%). The contributions of local pollutants like nitrate and HOA reduced, while the proportions of sulfate and OOA increased during the control episodes. The diurnal patterns of NR-PM1 species and OA components in each episode were characterized. The results showed that BC, nitrate, COA, and HOA had peaks in the morning and evening, which became less obvious under the emission control. Moreover, the diurnal variations of all species in Ep 3 with emission control were much flatter due to the effect of transport. Backward trajectories analysis confirmed the long-range transport of air masses from the continent, which resulted in the high proportions of sulfate (43.69%) and OOA (50.28%) in Ep 3. Our study implies the significant effect of emission control on reducing primary pollutants, but the formation of particles during the long-range transport need to be paid more attention when set the air quality control strategies in coastal cities.

8.
ACS Chem Neurosci ; 11(20): 3366-3376, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-32926778

RESUMO

Recent studies have revealed significant roles of neurotransmitters and gut microbiota along the gut-brain axis in Parkinson's disease (PD); however, the potential mechanisms remain poorly understood. In the current study, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced characteristic PD neurobehavior changes accompanied by increased α-synuclein, apoptotic protein Bim, and cleaved caspase-3 and decreased expression of tyrosine hydroxylase (TH). Meanwhile, the tryptophan (Trp) and tyrosine (Tyr) neurotransmitter metabolites involving kynurenine (KYN), serotonin (5-HT), and dopamine (DA) pathways were significantly changed in serum. Furthermore, the step-limited enzymes, which are responsible for the key metabolic pathways of these neurotransmitters, were obviously dysregulated. The 16S rRNA gene sequence results indicated that the abundance and diversity of the microbiota were obviously decreased in MPTP-treated mice, the presence of Ruminococcus, Parabacteroides and Parasutterella genera were obviously increased, while Coriobacteriaceae, Flavonifractor, Lachnospiraceae, Lactobacillaceae, and Rikenellaceae abundance was markedly decreased. The connectivity between the gut microbiota and neurotransmitter metabolism revealed that the gut microbiota dysbiosis was associated with disturbance of the DA, KYN, and 5-HT metabolic pathways. Therefore, our results provide evidence that gut-microbiota-brain axis disturbance may play an important role in PD development and targeting this axis might provide a promising therapeutic strategy for PD.

9.
Reg Anesth Pain Med ; 45(12): 955-963, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32963077

RESUMO

BACKGROUND AND OBJECTIVES: Gap junctions play a pivotal role in contributing to the formation of astroglial networks and in chronic pain. However, the mechanisms underlying the dysfunction of astroglial gap junctions in chronic pain have not been fully elucidated. METHODS: Chronic constriction injury (CCI) of the sciatic nerve was used to establish rat neuropathic pain model. C6 cells were used to perform experiments in vitro. Von Frey hairs and Hargreave's method were used to determine the withdrawal threshold of rats. Protein expression was detected by immunofluorescence and western blotting. RESULTS: Astragaloside IV (AST IV) significantly attenuated neuropathic pain and suppressed the excitation of spinal astrocytes in rats with CCI. The antinociceptive effect of AST IV was reversed by the gap junction decoupler carbenoxolone (CBX). AST IV inhibited the high expression of phosphorylated connexin 43 (p-Cx43) and p-c-Jun N-terminal kinase (p-JNK) in spinal cord of rats with CCI. JNK inhibitor alleviated neuropathic pain, which was reversed by CBX. JNK inhibitor decreased the high expression of p-Cx43 in both rats with CCI and tumor necrosis factor-alpha (TNF-α)-treated C6 cells. Additionally, the analgesic effect of AST IV was reversed by the adenosine triphosphate-sensitive potassium (KATP) channel blocker, glibenclamide (Glib). Glib abolished the inhibitory effects of AST IV on p-JNK and p-Cx43 both in vivo and in vitro. KATP channel opener (KCO) mimicked the inhibitory effects of AST IV on p-JNK and p-Cx43 in TNF-α-treated C6 cells. CONCLUSION: Our results indicate that the sciatic nerve CCI induces the dysfunction of gap junctions in the spinal cord by activating KATP/JNK signaling to contribute to neuropathic pain. AST IV attenuates neuropathic pain via regulating the KATP-JNK gap junction axis.

10.
Carbohydr Polym ; 249: 116812, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32933660

RESUMO

Using a sulfated polysaccharide from the gonad of pacific abalone (AGSP), a novel polysaccharide-Fe(III) complex, AGSP-Fe(III), was synthesized, and it was characterized by a series of methods including ultraviolet-visible (UV-vis) spectroscopy, Fourier transform-infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The results showed that AGSP-Fe(III) was formed by the Fe-O bond between sulfate, carboxyl, and hydroxyl groups in AGSP and Fe, its particle size reached a maximum of 200 nm after aggregating, and its surface morphology presented relatively regular columnar or spherical. Moreover, the iron release of AGSP-Fe(III) during simulated gastrointestinal digestion was exhibited, and its good iron supplementary efficiency was also shown using Caco-2 cells. In addition, compared to FeCl3, AGSP-Fe(III) showed better solubility and stability in the presence of polyphenol/trypsin. The present study demonstrated the potential of AGSP-Fe(III) as a novel iron supplement.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32830480

RESUMO

Interactions between the active components with the support are one of the fundamentally factors in determining the catalytic performance of a catalyst. In contrast to the comprehensive understanding on the strong metal-support interactions (SMSI) in metal-based catalysts, it remains unclear for the interactions among different oxides in mixed oxide catalysts due to its complexity. In this study, we investigated the interaction between CeO2 and LaFeO3, the two important oxygen storage materials in catalysis area, by tuning the sizes of CeO2 particles and highlight a two-fold effect of the strong oxide-oxide interaction in determining the catalytic activity and selectivity for preferential CO oxidation in hydrogen feeds. It is found that the anchoring of ultra-fine CeO2 particles (<2 nm) at the framework of three-dimensional-ordered macroporous LaFeO3 surface results in a strong interaction between the two oxides that induces the formation of abundant uncoordinated cations and oxygen vacancy at the interface, contributing to the improved oxygen mobility and catalytic activity for CO oxidation. Hydrogen spillover, which is an important evidence of the strong metal-support interactions in precious metal catalysts supported by reducible oxides, is also observed in the H2 reduction process of CeO2/LaFeO3 catalyst due to the presence of ultra-fine CeO2 particles (<2 nm). However, the strong interaction also results in the formation of surface hydroxyl groups, which when combined with the hydrogen spillover reduces the selectivity for preferential CO oxidation. This discovery demonstrates that in hybrid oxide-based catalysts, tuning the interaction among different components is essential for balancing the catalytic activity and selectivity.

14.
Food Funct ; 11(8): 7126-7137, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32749423

RESUMO

Food, nutrition, and pharmaceutical scientists are trying to elucidate the major factors impacting the bioavailability of macronutrients (e.g., lipids) and micronutrients (e.g., vitamins) so as to improve their efficacy. Currently, there is still a limited understanding of how food matrix effects impact digestion and bioaccessibility determined under the INFOGEST model, which is currently the most widely used standardized in vitro gastrointestinal model. Therefore, we examined the impact of corn oil concentration on lipid digestion and ß-carotene bioaccessibility using model food emulsions. For all oil concentrations tested (2.5 to 20%), complete lipid digestion was achieved using fed-state gastrointestinal conditions, which could only be seen if a back-titration was performed. The particle size and negative surface potential on the mixed micelles formed at the end of the small intestine phase both increased with increasing oil concentration, which was attributed to the generation of more free fatty acids. The ß-carotene bioaccessibility increased when the oil concentration was raised from 2.5 to 10% due to the increased solubilization capacity of the mixed micelles, but then it decreased when the oil concentration was raised further to 20% due to precipitation and sedimentation of some of the ß-carotene. The maximum ß-carotene bioaccessibility (93.2%) was measured at 10% oil. These results indicate that the oil concentration of emulsions influences ß-carotene bioaccessibility by altering digestion, solubilization, and precipitation processes. This knowledge is important when designing more effective functional or medical food products.

15.
J Food Sci ; 85(9): 2822-2831, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32794226

RESUMO

The anti-inflammatory effects of two newly identified Pleurotus eryngii polysaccharides (WPEP, NPEP) were determined in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in this study. Characterization analysis revealed that molecular weights of WPEP and NPEP were 167 and 274 kDa, and were mainly composed of glucose with ß-type glycosidic linkages. WPEP and NPEP could significantly inhibit LPS-induced inflammatory responses by regulating the production of NO, Protaglandin E2 (PGE2 ), Interleukin-1ß (IL-1ß), Tumor necrosis factor-α (TNF-α), and Interleukin-6 (IL-6). This was through the blocking of the activation of Mitogen-activated protein kinase (MAPK) pathway by inhibiting phosphorylation of p38, extracellular regulation of protein kinases 1/2, and stress-activated protein kinase/jun aminoterminal kinase. Moreover, WPEP and NPEP inhibited NF-κB signaling by reducing nuclear translocation and phosphorylation of p65. Overall, our results, for the first time identified two P. eryngii polysaccharides and demonstrated the related anti-inflammatory effects, which indicated the favorable potential of P. eryngii polysaccharide as specific functional foods. PRACTICAL APPLICATION: This study prepared and characterized newly identified Pleurotus eryngii water-soluble polysaccharide fractions and elucidated the nutritional benefits, mainly the immune response related to anti-inflammatory activities by utilizing lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Collectively, results of this study suggested that the P. eryngii polysaccharide fractions could be considered as potential candidates for exploration in the development of new immunomodulatory agent or functional supplementary foods.


Assuntos
Anti-Inflamatórios/farmacologia , Extratos Vegetais/farmacologia , Pleurotus/química , Polissacarídeos/farmacologia , Animais , Anti-Inflamatórios/química , Dinoprostona/imunologia , Interleucina-6/genética , Interleucina-6/imunologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , NF-kappa B/genética , NF-kappa B/imunologia , Extratos Vegetais/química , Polissacarídeos/química , Células RAW 264.7 , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia
16.
Toxicol Lett ; 333: 150-158, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32768653

RESUMO

Methamphetamine (Meth), a highly addictive drug, can induce irreversible neuronal damage and cause neuropsychiatric and cognitive disorders. Meth's effects on modulating microglial neuroimmune functions and eliciting neuroinflammation have attracted considerable attention in recent years. Recent evident of the effect of the non-dependent domain containing adaptor inducing interferon (TRIF)/Pellino1 (Peli1) signaling axis on pro-inflammatory cytokine production provides novel clues for inflammation. Therefore, our study investigated Meth-induced neurotoxicity from a neuropathological perspective by examining TLR4-TRIF-Peli1 axis signaling activation. Meth significantly activated microglia accompanied by marked increase of TLR4 and TRIF expression, NF-kB and MAPK pathways activation and the production of IL-1ß, TNF-α and IL-6. Peli1 was involved in Meth-mediated neuroinflammation and knockdown of Peli1 strongly reversed NF-kB and MAPK pathways activation and pro-inflammatory cytokine excretion. Intriguingly, Peli1 upregulation induced by Meth was dependent on TRIF rather than the myloid differentiation factor 88 (MyD88) pathway, since the silencing of TRIF significantly suppressed Meth-induced Peli1 upregulation, while MyD88 knockdown had no obvious impact. Additionally, an in vivo study verified TLR4-TRIF-Peli1 axis activation and an enhanced level of downstream cytokine expression in the cortex after Meth treatment. Therefore, these findings provide new insight regarding the specific contributions of the TRIF-Peli1 pathway to Meth-mediated neuroinflammation.


Assuntos
Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Encéfalo/efeitos dos fármacos , Estimulantes do Sistema Nervoso Central/toxicidade , Metanfetamina/toxicidade , Microglia/efeitos dos fármacos , Proteínas Nucleares/metabolismo , Receptor 4 Toll-Like/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Proteínas Adaptadoras de Transporte Vesicular/genética , Animais , Encéfalo/imunologia , Encéfalo/metabolismo , Técnicas de Cultura de Células , Linhagem Celular , Citocinas/genética , Expressão Gênica/efeitos dos fármacos , Técnicas de Silenciamento de Genes , Masculino , Camundongos Endogâmicos C57BL , Microglia/imunologia , Microglia/metabolismo , Proteínas Nucleares/genética , RNA Interferente Pequeno/genética , Transdução de Sinais , Receptor 4 Toll-Like/genética , Ubiquitina-Proteína Ligases/genética , Regulação para Cima
17.
J Agric Food Chem ; 68(32): 8688-8701, 2020 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-32633953

RESUMO

Obesity is associated with gut microbiome dysbiosis. Our previous research has shown that highly branched rhamnogalacturonan type I (RG-I)-enriched pectin (WRP, 531.5 kDa, 70.44% RG-I, Rha/(Gal + Ara) = 20) and its oligosaccharide with less branched RG-I [DWRP, 12.1 kDa, 50.29% RG-I, Rha/(Gal + Ara) = 6] are potential prebiotics. The present study is conducted to uncover the impact of the content, molecular size, and branch degrees of RG-I on the inhibiting effect of high-fat diet (HFD)-induced obesity. The commercial pectin (CP, 496.2 kDa, 35.77% RG-I, Rha/(Gal + Ara) = 6), WRP, and DWRP were orally administered to HFD-fed C57BL/6J mice (100 mg kg-1 d-1) to determine their individual effects on obesity. WRP significantly prevented bodyweight gain, insulin resistance, and inflammatory responses in HFD-fed mice. No obvious anti-obesity effect was observed in either CP or DWRP supplementation. A mechanistic study revealed that CP and DWRP could not enhance the diversity of gut microbiota, while WRP treatment positively modulated the gut microbiota of obese mice by increasing the abundance of Butyrivibrio, Roseburia, Barnesiella, Flavonifractor, Acetivibrio, and Clostridium cluster IV. Furthermore, WRP significantly promoted browning of white adipose tissues in HFD-fed mice, while CP and DWRP did not. WRP can attenuate the HFD-induced obesity by modulation of gut microbiota and lipid metabolism. Highly branched RG-I domain enrichment is essential for pectin mitigating against the HFD-induced obesity.

18.
BMC Cardiovasc Disord ; 20(1): 324, 2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32631247

RESUMO

BACKGROUND: De Winter pattern is associated with acute occlusion in the left anterior descending coronary artery combined with upsloping ST-segment depression at the J point in leads V1 through V6 without ST-segment elevation. The ECG changes in this case were illustrated by an up-sloping ST-segment depression in the V1 to V6 leads, followed by tall and symmetrical T waves. Changes from de Winter to ST-segment elevation myocardial infarction (STEMI) are rare. CASE PRESENTATION: Our case illustrated an evolutionary de Winter sign that changed to STEMI; the patient underwent cardiac catheterization in time. CONCLUSIONS: Patients who have an electrocardiogram showing de Winter changes may require primary percutaneous coronary intervention. Emergency physicians and cardiologists should not ignore these changes.

19.
FASEB J ; 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32667083

RESUMO

Integrated metabolomics and proteomics analysis was carried out to study the effects of Poria and its split components (volatile oil, triterpenoid, oligosaccharide, amino acid, and crude polysaccharide) on rats of normal physiological model, hyperthyroidism model, and hypothyroidism model to explore the substance basis of Poria for hypothyroidism from the perspective of a holistic view in substance and energy metalism. The key pathways regulating substance and energy metabolism were screened, encompassing tricarboxylic acid cycle pathway, glycolysis/ gluconeogenesis pathways, biosynthesis of amino acid pathway, fatty acid biosynthesis pathway, pentose phosphate pathway, peroxisome proliferator-activated receptors pathway, etc Poria and its split components showed promoting effects on substance and energy metabolism in normal model, while showed amelioration effects on hypothyroidism model at different degrees, and had no significant improvement effects on hyperthyroidism in rats. Volatile oil, triterpenoid, and crude polysaccharide from Poria were regarded as substance basis of Poria ameliorating hypothyroidism other than hyperthyroidism. This work also revealed the feasibility of metabolomics and proteomics analysis to elucidate the effective substance basis of traditional Chinese medicine from a new viewpoint based on its effects on substance and energy metabolism.

20.
Kaohsiung J Med Sci ; 36(11): 929-936, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32643870

RESUMO

Acute lung injury (ALI) caused by gas explosion is common, and warrants research on the underlying mechanisms. Specifically, the role of abnormalities of coagulation and fibrinolysis in this process has not been defined. It was hypothesized that the abnormal coagulation and fibrinolysis promoted ALI caused by gas explosion. Based on the presence of ALI, 74 cases of gas explosion injury were divided into the ALI and non-ALI groups. The results of prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), and platelet count (PLT) were collected within 24 hours and compared between the groups. ALI models caused by gas explosion were established in Sprague Dawley rats, and injuries were evaluated using hematoxylin and eosin (HE) staining and histopathological scoring. Moreover, the bronchoalveolar lavage fluid (BALF) was collected to examine thrombin-antithrombin complex (TAT), tissue factor (TF), tissue factor pathway inhibitor (TFPI), and plasminogen activator inhibitor-1 (PAI-1) levels by enzyme-linked immunosorbent assay (ELISA). The patients in ALI group had shorter PT and longer APTT, raised concentration of FIB and decreased number of PLT, as compared to the non-ALI group. In ALI rats, the HE staining revealed red blood cells in alveoli and interstitial thickening within 2 hours which peaked at 72 hours. The levels of TAT/TF in the BALF increased continually until the seventh day, while the PAI-1 was raised after 24 hours and 7 days. The TFPI was elevated after 2 hours and 24 hours, and then decreased after 72 hours. Abnormalities in coagulation and fibrinolysis in lung tissues play a role in ALI caused by gas explosion.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA