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1.
Neural Regen Res ; 16(1): 150-157, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32788470

RESUMO

Acrylamide has been shown to be neurotoxic. Brain-derived neurotrophic factor (BDNF) can alleviate acrylamide-induced synaptic injury; however, the underlying mechanism remains unclear. In this study, dibutyryl-cyclic adenosine monophosphate-induced mature human neuroblastoma (NB-1) cells were exposed with 0-100 µg/mL acrylamide for 24-72 hours. Acrylamide decreased cell viability and destroyed synapses. Exposure of co-cultured NB-1 cells and Schwann cells to 0-100 µg/mL acrylamide for 48 hours resulted in upregulated expression of synapsin I and BDNF, suggesting that Schwann cells can activate self-protection of neurons. Under co-culture conditions, activation of the downstream TrkB-MAPK-Erk1/2 pathway strengthened the protective effect. Exogenous BDNF can increase expression of TrkB, Erk1/2, and synapsin I, while exogenous BDNF or the TrkB inhibitor K252a could inhibit these changes. Taken together, Schwann cells may act through the BDNF-TrkB-MAPK-Erk1/2 signaling pathway, indicating that BDNF plays an important role in this process. Therefore, exogenous BDNF may be an effective treatment strategy for acrylamide-induced nerve injury. This study was approved by the Laboratory Animal Welfare and Ethics Committee of the National Institute of Occupational Health and Poison Control, a division of the Chinese Center for Disease Control and Prevention (approval No. EAWE-2017-008) on May 29, 2017.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33042012

RESUMO

Neuropeptides are the most abundant and diverse signal molecules in insects. They act as neurohormones and neuromodulators to regulate the physiology and behavior of insects. The majority of neuropeptides initiate downstream signaling pathways through binding to G protein-coupled receptors (GPCRs) on the cell surface. In this study, RNA-seq technology and bioinformatics were used to search for genes encoding neuropeptides and their GPCRs in the cowpea aphid Aphis craccivora. And the expression of these genes at different developmental stages of A. craccivora was analyzed by quantitative real-time PCR (qRT-PCR). A total of 40 candidate genes encoding neuropeptide precursors were identified from the transcriptome data, which is roughly equivalent to the number of neuropeptide genes that have been reported in other insects. On this basis, software analysis combined with homologous prediction estimated that there could be more than 60 mature neuropeptides with biological activity. In addition, 46 neuropeptide GPCRs were obtained, of which 40 belong to rhodopsin-like receptors (A-family GPCRs), including 21 families of neuropeptide receptors and 7 orphan receptors, and 6 belong to secretin-like receptors (B-family GPCRs), including receptors for diuretic hormone 31, diuretic hormone 44 and pigment-dispersing factor (PDF). Compared with holometabolous insects such as Drosophila melanogaster, the coding genes for sulfakinin, corazonin, arginine vasopressin-like peptide (AVLP), and trissin and the corresponding receptors were not found in A. craccivora. It is speculated that A. craccivora likely lacks the above neuropeptide signaling pathways, which is consistent with Acyrthosiphon pisum and that the loss of these pathways may be a common feature of aphids. In addition, expression profiling revealed neuropeptide genes and their GPCR genes that are differentially expressed at different developmental stages and in different wing morphs. This study will help to deepen our understanding of the neuropeptide signaling systems in aphids, thus laying the foundation for the development of new methods for aphid control targeting these signaling systems.

3.
Analyst ; 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33016277

RESUMO

The polypeptide antibiotics colistin (COL) and bacitracin (Baci) are extensively used as veterinary drugs and feedstock additives in the livestock industry, which inevitably causes residues in animal-origin food, which can accelerate human tolerance to antibiotics. In this study, a portable lateral flow immunoassay (LFIA) for the simultaneous determination of COL and Baci residues in milk was developed. The replacement of gold nanoparticles used in the traditional LFIA with fluorescent microspheres (FMs) to label monoclonal antibodies (mAbs) allowed qualitative and quantitative analyses within a few minutes. Based on the principle of competitive binding to FM-labelled mAbs between analytes in samples and fixed antigens on the membrane, the assay provided qualitative cut-off values of 100 and 50 ng mL-1 for Baci and COL in milk samples. Furthermore, a strip reader-based semi-quantitative detection system could detect lower limits of 7.85 and 1.89 ng mL-1 for Baci and COL, respectively. In conclusion, the proposed multiplex LFIA immunosensor provides an auxiliary analytical tool for the rapid and simultaneous screening of COL and Baci in large cohorts of samples.

4.
Amino Acids ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33021685

RESUMO

Corynebacterium glutamicum has a long and successful history in the biotechnological production of L-lysine. Besides the adjustment of metabolic pathways, intracellular and extracellular transport systems are critical for the cellular metabolism of L-lysine or its by-products. Here, three amino acid transmembrane transporters, namely, GluE, BrnE/BrnF, and LysP, which are widely present in C. glutamicum strains, were each investigated by gene knockout. In comparison with that in the wild-type strain, the yield of L-lysine increased by 9.0%, 12.3%, and 10.0% after the deletion of the gluE, brnE/brnF, and lysP genes, respectively, in C. glutamicum 23,604. Moreover, the amount of by-product amino acids decreased significantly when the gluE and brnE/brnF genes were deleted. It was also demonstrated that there was no effect on the growth of the strain when the gluE or lysP gene was deleted, whereas the biomass of C. glutamicum WL1702 (ΔbrnE/ΔbrnF) in the fermentation medium was significantly reduced in comparison with that of the wild type. These results also provide useful information for enhancing the production of L-lysine or other amino acids by C. glutamicum.

5.
J Gen Intern Med ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33021715

RESUMO

BACKGROUND: Strict medication guidance and lifestyle interventions to manage blood pressure (BP) in hypertensive patients are typically difficult to follow. OBJECTIVE: To evaluate the 1-year effectiveness of lifestyle and drug intervention in the management of rural hypertensive patients. DESIGN: Randomized community intervention trial. PARTICIPANTS: The control group comprised 967 patients who received standard antihypertensive drug intervention therapy from two communities, whereas the intervention group comprised 1945 patients who received antihypertensive drug and lifestyle intervention therapies from four communities in rural China. MAIN MEASURES: Data on lifestyle behaviors and BP measurements at baseline and 1-year follow-up were collected. A difference-in-difference logistic regression model was used to assess the effect of the intervention. KEY RESULTS: BP control after the 1-year intervention was better than that at baseline in both groups. The within-group change in BP control of 59.3% in the intervention group was much higher than the 25.2% change in the control group (P < 0.001). Along with the duration of the follow-up period, systolic and diastolic BP decreased rapidly in the early stages and then gradually after 6 months in the intervention group (P < 0.001). In the intervention group, drug therapy adherence was increased by 39.5% (from 48.1% at 1 month to 87.6% at 1 year) (P < 0.001), more in women (45.6%) than in men (31.2%; P < 0.001). The net effect of the lifestyle intervention improved the rate of BP control by 56.1% (70.8% for men and 44.7% for women). For all physiological and biochemical factors, such as body mass index, waist circumference, lipid metabolism, and glucose control, improvements were more significant in the behavioral intervention group than those in the control group (all P < 0.001). CONCLUSION: The addition of lifestyle intervention by physicians or nurses helps control BP effectively and lowers BP better than usual care with antihypertensive drug therapy alone.

6.
Med (N Y) ; 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33043313

RESUMO

Background: The coronavirus disease 2019 (COVID-19) is an emerged respiratory infectious disease with kidney injury as a part of the clinical complications. However, the dynamic change of kidney function and its association with COVID-19 prognosis are largely unknown. Methods: In this multicenter retrospective cohort study, we analyzed clinical characteristics, medical history, laboratory tests, and treatment data of 12,413 COVID-19 patients. The patient cohort was stratified according to the severity of the outcome into three groups: non-severe, severe, and death. Findings: The prevalence of elevated blood urea nitrogen (BUN), elevated serum creatinine (Scr), and decreased blood uric acid (BUA) at admission was 6.29%, 5.22%, 11.66%, respectively. The trajectories showed elevation of BUN level and Scr level, as well as a reduction of BUA level during 28 days after admission in death cases. Increased all-cause mortality risk was associated with elevated baseline levels of BUN and Scr, and decreased level of BUA. Conclusion: The dynamic changes of the three kidney function markers were associated with different severity and poor prognosis of COVID-19 patients. BUN showed close association and high potential for predicting adverse outcomes in COVID-19 patients for severity stratification and triage.

7.
Int J Hyperthermia ; 37(1): 1174-1181, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33043742

RESUMO

PURPOSE: To explore the association of demographic characteristics, clinical symptoms and the fear of the disease progression factors with the physical and mental summary components of the health-related of life (HRQoL) of the papillary thyroid microcarcinoma (PTMC) patients undergoing radiofrequency ablation (RFA). METHODS: 123 PTMC survivors undergoing RFA were enrolled in this study from October 2019 to March 2020. Demographic, clinical symptoms and the fear of the disease progression data were collected. SF-36, THYCA-QoL and FoP-Q-SF were used to evaluate the HRQoL of patients, clinical symptoms and the fear of disease progression. A multivariate regression model was performed to evaluate the association between the independent variable and the HRQoL variable. RESULTS: The average self-reported HRQoL score was 81.17 ± 15.48 for the PCS and 73.40 ± 18.03 for the MCS. The multivariate linear regression model shows that the factors related to a poorer PCS were dependent for the female patients, the symptoms of neuromuscular and the throat/mouth, the fear of disease progression; the psychological disorder, symptoms of throat/mouth, inability to concentrate were related to worse scores for the MCS. The condition that was most strongly related to a poorer HRQoL (in both PCS and MCS) was the fear of their physical health. CONCLUSIONS: The factors related to significantly worse HRQoL scores across PCS and MCS for PTMC survivors include the female gender, the symptoms of neuromuscular and the throat/mouth, the psychological disorder, inability to concentrate, and the fear of their own physical health. Identification, management, and prevention of these factors are critical to improving the HRQoL of patients.

8.
Artigo em Inglês | MEDLINE | ID: mdl-33085921

RESUMO

Background: Tumor microenvironment (TME) significantly affects colorectal cancer (CRC) progression and therapeutic efficacy, particularly the infiltrating stromal components. This study aimed to profile the TME composition of tumor tissue and identify TME-related, especially stroma-related genes having prognosis value in CRC patients. Materials and Methods: We used ESTIMATE algorithm to assess stromal/immune component and divided 524 CRC cases of public dataset into high- and low-score groups. We analyzed effect of the score on prognosis and extracted the differential expression genes (DEGs) between groups, which were stromal- and/or immune-related genes, and performed prognostic investigation of the DEGs. Results: Higher stromal score was correlated with poor survival, whereas immune score was the opposite. By comparing global gene expression of cases with high vs. low stromal/immune scores, we extracted 474 stroma-related genes, 76 immune-related genes, and 498 intersection genes, which were explored by function enrichment and survival analysis. We identified the expression of five stroma-related genes (including ITGA7, PTPN14, SCG2, TNS1, and GRP) significantly associated with poorer survival, which were validated in the other two independent CRC cohorts. Conclusion: These results presented a comprehensive understanding of TME components and identified five stroma-related genes that predict poor outcomes in CRC patients.

9.
Mol Cancer Res ; 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33087417

RESUMO

Resistance to immunotherapy is a significant challenge, and the scarcity of human models hinders the identification of the underlying mechanisms. To address this limitation, we constructed an autologous humanized mouse (aHM) model with hematopoietic stem and progenitor cells (HSPCs) and tumors from two melanoma patients progressing to immunotherapy. Unlike mismatched humanized mouse (mHM) models, generated from cord blood-derived HSPCs and tumors from different donors, the aHM recapitulates a patient-specific tumor microenvironment (TME). When patient tumors were implanted on aHM, mHM and NOD/SCID/IL2rg-/- (NSG) cohorts, tumors appeared earlier and grew faster on NSG and mHM cohorts. We observed that immune cells differentiating in the aHM were relatively more capable of circulating peripherally, invading into tumors and interacting with the TME. A heterologous, human leukocyte antigen (HLA-A) matched cohort also yielded slower growing tumors than non-HLA-matched mHM, indicating that a less permissive immune environment inhibits tumor progression. When the aHM, mHM, and NSG cohorts were treated with immunotherapies mirroring what the originating patients received, tumor growth in the aHM accelerated, similar to the progression observed in the patients. This rapid growth was associated with decreased immune cell infiltration, reduced interferon gamma (IFNγ)-related gene expression, and a reduction in STAT3 phosphorylation, events that were replicated in vitro using tumor-derived cell lines. Implications: Engrafted adult HSPCs give rise to more tumor infiltrative immune cells, increased HLA matching leads to slower tumor initiation and growth, and continuing immunotherapy past progression can paradoxically lead to increased growth.

11.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(5): 1674-1678, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33067972

RESUMO

OBJECTIVE: To explore the correlation between the of regulatory T cells, Th17 cells and the prognosis of children with aplastic anemia. METHODS: The clinical data 13 children with aplastic anemia (AA) treated by antithymocyte globulin (ATG) combined with Cyclosporine in Beijing Children's Hospital, Capital Medical University from January 2017 to January 2018 were analyized retrospectively. The changes of T cell and Th17 cell expression level in peripheral blood of AA children before and after IST for 6 and 12 month were compared and analyzed. The SPSS 19.0 statistical package was used for data analysis. RESULTS: Compared with the pre-IST, the expression level of Treg cells decreased at 6 months of IST, the difference was statistically significant (P<0.05); however the expression level of Th17 cells did not show significant difference as compared with that pre-IST. The expression level of Treg and Th17 cells at 12 months of IST was lower than that pre-IST (P<0.01), compared with the level pre-IST, the ratio of Treg cells/Th17 cell at 6 months and 12 months of IST did not show a singificand difference. CONCLUSION: Treg cells and Th17 cells in peripheral blood of AA children decrease after IST, which suggests that the change of regulatory T cells and Th17 cells correlate with the clinical outcome of children with aplastic anemia.


Assuntos
Anemia Aplástica , Linfócitos T Reguladores , Criança , Humanos , Prognóstico , Estudos Retrospectivos , Células Th17
12.
World J Clin Cases ; 8(19): 4550-4557, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33083417

RESUMO

BACKGROUND: Gastro-thoracic fistula is a serious complication after radical surgery for esophageal cancer, and a conservative approach or endoscopic intervention is commonly applied to treat most cases. CASE SUMMARY: Here we describe the case of a patient with a gastro-thoracic fistula which could not be closed during gastroscopy after receiving postoperative radiotherapy, together with severe multiple drug-resistant bacterial infection and chest wall fistula. The abscess was drained and local irrigation applied with ozonated water, together with oral ozonated water, which achieved a good effect and highlighted a new way to cure fistula in such patients. CONCLUSION: Patients with gastro-thoracic fistula that cannot be closed and severe infection can be treated by drainage and flushing with ozonated water.

13.
Anal Methods ; 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33084636

RESUMO

Contamination of grains and related products by fumonisins (FBs) is increasingly becoming a serious food security issue. The aim of this work was to develop a europium fluorescent microsphere-based time-resolved fluorescence immunochromatographic assay (TRFICA) for FB1 detection in different grains, including corn, corn flour, wheat, rice and brown rice. Standard curves for the five types of grain matrix were established, and showed good linearity (R2 > 0.975), LOD of 8.26 µg kg-1, and a wide working range of 13.81-1000 µg kg-1. The recoveries of TRFICA for FB1 detection ranged from 82.85-103.62% with variation coefficients of 1.92-15.33%. Two corn reference materials and other natural samples were tested using TRFICA. The same samples analyzed by liquid chromatography tandem mass spectrometry further confirmed the TRFICA results. The entire detection time of TRFICA was within 30 min. Thus, this developed TRFICA can be used for onsite detection and quantitation of FB1 in grains.

14.
Shanghai Kou Qiang Yi Xue ; 29(4): 370-374, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-33089284

RESUMO

PURPOSE: To investigate the role of P2X7 receptor (P2X7r) in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). METHODS: hPDLSCs were isolated from the premolars collected in the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, and divided into four groups. Group A was cultured in conventional medium, group B was cultured in osteogenic induction medium, group C was cultured in osteogenic induction medium + 100 nmol/L adenosine triphosphate (ATP) solution, and group D was cultured in osteogenic induction medium + 100 nmol/L P2X7 receptor specific antagonist KN-62. After 7 days, alizarin red staining was used to observe the osteogenic effect of hPDLSCs in each group. The mRNA expression of osteocalcin (OCN), RUNX2 and P2X7r in hPDLSCs was detected by real-time PCR reaction (RT-PCR). The data were processed by SPSS 22.0 software package. RESULTS: Alisarin red staining showed that the morphology of hPDLSCs cells in group B and group C was significantly changed. The pale calcified nodules in group C were significantly more than those in group B, while very few calcified nodules were found in group A and group D. The mRNA expression of OCN, RUNX2 and P2X7r in hPDLSCs were the highest in group C, followed by group B(P<0.05), and no difference was found between group A and group D(P>0.05). CONCLUSIONS: P2X7 receptor can promote osteogenic differentiation of human periodontal ligament stem cells after being activated by ATP, which may provide a new direction for clinical treatment of periodontitis.


Assuntos
Ligamento Periodontal , Receptores Purinérgicos P2X7 , China , Humanos , Osteogênese/genética , Receptores Purinérgicos P2X7/genética , Células-Tronco
15.
Med Image Anal ; 67: 101839, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33080508

RESUMO

The interpretation of medical images is a complex cognition procedure requiring cautious observation, precise understanding/parsing of the normal body anatomies, and combining knowledge of physiology and pathology. Interpreting chest X-ray (CXR) images is challenging since the 2D CXR images show the superimposition on internal organs/tissues with low resolution and poor boundaries. Unlike previous CXR computer-aided diagnosis works that focused on disease diagnosis/classification, we firstly propose a deep disentangled generative model (DGM) simultaneously generating abnormal disease residue maps and "radiorealistic" normal CXR images from an input abnormal CXR image. The intuition of our method is based on the assumption that disease regions usually superimpose upon or replace the pixels of normal tissues in an abnormal CXR. Thus, disease regions can be disentangled or decomposed from the abnormal CXR by comparing it with a generated patient-specific normal CXR. DGM consists of three encoder-decoder architecture branches: one for radiorealistic normal CXR image synthesis using adversarial learning, one for disease separation by generating a residue map to delineate the underlying abnormal region, and the other one for facilitating the training process and enhancing the model's robustness on noisy data. A self-reconstruction loss is adopted in the first two branches to enforce the generated normal CXR image to preserve similar visual structures as the original CXR. We evaluated our model on a large-scale chest X-ray dataset. The results show that our model can generate disease residue/saliency maps (coherent with radiologist annotations) along with radiorealistic and patient specific normal CXR images. The disease residue/saliency map can be used by radiologists to improve the CXR reading efficiency in clinical practice. The synthesized normal CXR can be used for data augmentation and normal control of personalized longitudinal disease study. Furthermore, DGM quantitatively boosts the diagnosis performance on several important clinical applications, including normal/abnormal CXR classification, and lung opacity classification/detection.

16.
Ann Neurol ; 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33016348

RESUMO

NOTCH2NLC GGC repeat expansions were recently identified in neuronal intranuclear inclusion disease (NIID); however, it remains unclear whether they occur in other neurodegenerative disorders. This study aimed to investigate the role of intermediate-length NOTCH2NLC GGC repeat expansions in Parkinson disease (PD). We screened for GGC repeat expansions in a cohort of 1,011 PD patients and identified 11 patients with intermediate-length repeat expansions ranging from 41 to 52 repeats, with no repeat expansions in 1,134 controls. Skin biopsy revealed phospho-alpha-synuclein deposition, confirming the PD diagnosis in 2 patients harboring intermediate-length repeat expansions instead of NIID or essential tremor. Fibroblasts from PD patients harboring intermediate-length repeat expansions revealed NOTCH2NLC upregulation and autophagic dysfunction. Our results suggest that intermediate-length repeat expansions in NOTCH2NLC are potentially associated with PD. ANN NEUROL 2020.

17.
Sci Adv ; 6(42)2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33067230

RESUMO

Inputs of nitrogen into terrestrial ecosystems, mainly via the use of ammonium-based fertilizers in agroecosystems, are enormous, but the fate of this nitrogen under elevated atmospheric carbon dioxide (CO2) is not well understood. We have taken advantage of a 15-year free-air CO2 enrichment study to investigate the influence of elevated CO2 on the transformation of ammonium-nitrogen in a rice ecosystem in which ammonium is usually assumed to be stable under anaerobic conditions. We demonstrate that elevated CO2 causes substantial losses of ammonium-nitrogen that result from anaerobic oxidation of ammonium coupled to reduction of iron. We identify a new autotrophic member of the bacterial order Burkholderiales that may use soil CO2 as a carbon source to couple anaerobic ammonium oxidation and iron reduction. These findings offer insight into the coupled cycles of nitrogen and iron in terrestrial ecosystems and raise questions about the loss of ammonium-nitrogen from arable soils under future climate-change scenarios.

18.
Medicine (Baltimore) ; 99(41): e22663, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33031330

RESUMO

RATIONALE: GNE myopathy is caused by mutations in the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase(GNE) gene and is clinically characterized by progressive weakness and atrophy of the lower-limb muscles with quadriceps sparing. Nearly all GNE mutations that have been reported thus far in various ethnic populations around the world have been missense or nonsense mutations. PATIENT CONCERNS: We describe the case of a 32-year-old woman with GNE myopathy. The patient presented with progressive weakness of the lower-limb muscles that had spread to her legs. Her serum creatine kinase level was higher than the normal range. Mild myogenic changes were detected in the tibialis anterior muscles on electromyography, and moderate fatty infiltration was observed in various lower-limb muscles on magnetic resonance imaging. Histopathological examination of a skeletal muscle biopsy specimen revealed variation in muscle fiber size, rimmed vacuoles, and disorganized intermyofibrillar networks. DNA sequencing testing revealed a compound heterozygous mutation consisting of a known mutation (c.620A > T in exon 3) and a novel (exon 1 deletion) mutation. DIAGNOSES: Taken together, the clinical features, laboratory testing and DNA findings eventually made the diagnosis of GNE myopathy. INTERVENTIONS AND OUTCOMES: Based on the diagnosis of the GNE myopathy, the patient was administered sialic acid 6 g a day for 1 year, and up to now, her symptoms did not progress further. LESSONS: We have reported the case of a GNE myopathy patient with compound heterozygous GNE gene mutations. This case expands the genotypic spectrum of GNE myopathy.


Assuntos
Complexos Multienzimáticos/genética , Doenças Musculares/genética , Adulto , Grupo com Ancestrais do Continente Asiático , Feminino , Humanos , Deleção de Sequência
19.
Ren Fail ; 42(1): 932-943, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32909490

RESUMO

AIM: When peritoneal fibrosis (PF) causes ultrafiltration failure in peritoneal dialysis (PD) patients, PD has to be discontinued. Currently, there is no effective way to relieve PF. In this study, we aimed to determine whether miR-15a-5p is involved in PF and to determine the underlying mechanism. METHODS: Six normal rats were used as the control group. A uremic rat model was constructed using 5/6 nephrectomy in a Sprague-Dawley model. The uremic rats were randomly divided into PD, lentivirus-transfected, negative control, VEGFR-inhibited and gavage control groups. Except for the control group, all uremia rats received continuous PD for 28 days. In the lentivirus-transfected group, the miR-15a-5p plasmid was injected into the peritoneal cavity to upregulate miR-15a-5p expression. Axitinib was used to block vascular endothelial growth factor receptor (VEGFR) in the peritoneum. The mRNA levels of miR-15a-5p and VEGF were detected by qRT-PCR and FISH. Protein levels of VEGF, E-cadherin, collagen IV, fibronectin and α-SMA were detected by western blot and immunohistochemistry. RESULTS: PD leads to peritoneal thickening and fibrosis. The expression level of miR-15a-5p decreased and that of VEGF increased in the PD group than in the controls. Additionally, E-cadherin was significantly reduced while collagen IV, fibronectin and α-SMA were obviously increased in the PD group compared to controls. FISH showed that VEGF might be the target gene of miR-15a-5p. Overexpression of miR-15a-5p or inhibition of VEGFR could reverse PF. CONCLUSION: miR-15a-5p may participate in the endothelial to mesenchymal transition of PF caused by PD through VEGF.

20.
Anal Methods ; 12(25): 3210-3217, 2020 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-32930183

RESUMO

Dinitolmide is a nitro amide coccidiostat used in poultry feed, and is a potential threat to the environment and human health. In this study, a monoclonal antibody (mAb) against dinitolmide was prepared and an immunochromatographic assay (ICA) was developed to detect residual dinitolmide in chicken tissue. The results show that the mAb exhibited high sensitivity, with a limit of detection as low as 9.01 ng mL-1. A cross reactivity test revealed that the mAb also had good specificity for dinitolmide. This ICA method showed a visible limit of detection of 2.5 µg kg-1, and a cut-off value of 25 µg kg-1 for testing dinitolmide in chicken sample extract by using the naked eye. Importantly, these observations using our ICA, were comparable to other methods of detection such as liquid chromatography, tandem mass spectrometry and indirect competitive enzyme-linked immunosorbent assays (IC-ELISA). These data suggest that our ICA method is a reliable, portable, and high-throughput method for the detection of dinitolmide residues in chicken tissue.

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