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1.
J Nanosci Nanotechnol ; 20(1): 64-80, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383140

RESUMO

The conductivity of Geobacter sulfurreducens is attributed mainly to its truncated pili, known as microbial nanowires. In this study, we explored the biological factors that limit electron transfer and hence the conductivity of pili, including the types of aromatic residue, distances between aromatic residues, local electrostatic environment around aromatic residues, and percentage of aromatic residues in the pilin subunits that form the pili, as well as the physico-chemical interactions in the pili, by comparing the structures of pili with different conductivities in electricigens. Structures of the Geobacter pili and their mutants were constructed using the symmetric docking module of the Rosetta software. Potential electron transfer pathways in the pili were identified based on Dijkstra's shortest pathway algorithm. We found that the conductivity of full-length pili could be increased when the hydrophobic C-terminal spheres of pilin proteins are truncated. The mutant pili with altered aromatic residues probably have higher conductivity than wild-type, when the interactions between the α-N domains of pilins are enhanced. A larger percentage of aromatic residues in the N-termini of the pilin subunits resulted in higher conductivity of the corresponding pili. These results provide new insights about strategies for synthesizing high electrically conductive nanowires.

2.
Math Biosci Eng ; 16(5): 5788-5803, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31499738

RESUMO

In this study, the radial basis function (RBF) which has good performance for nonlinear problem is introduced to approximate the implicit relationships between EDM parameters and performance responses for 304 steel. The fitting precision of RBF is compared with the second order polynomial response surface (PRS), support vector regression (SVR) and Kriging model (KRG) using the multiple correlation coefficient (R2) based cross validation error method. Then the RBF model is called to conduct multi-objective optimization using non-dominated sorting genetic algorithm II (NSGA-II) method. The energy consumption index unit energy consumption (UEC) and the air-pollution indices PM2.5 and PM10 are considered in proposed multi-objective optimization model. UEC is considered as the objective function to reduce the machining cost and the PM indices are termed as the constraints to protect the operators' health. The pulse current, time period and duty cycle are considered as the main factors affecting the EDM responses. According to the Pareto plots of multi-objective optimization model, conclusion can be drawn that SR and PM10 play significant roles in multi-optimization and PM2.5 has less influence on optimization results. The results of the present study reveal that using maximum material removal rate (MRR) and minimum UEC as objective and using surface roughness (SR), PM2.5 and PM10 as constraints can be an effective method to provide appropriate process parameters reference for EDM machining.

3.
Adv Mater ; : e1904243, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31456250

RESUMO

Ruddlesden-Popper perovskites (RPPs), consisting of alternating organic spacer layers and inorganic layers, have emerged as a promising alternative to 3D perovskites for both photovoltaic and light-emitting applications. The organic spacer layers provide a wide range of new possibilities to tune the properties and even provide new functionalities for RPPs. However, the preparation of state-of-the-art RPPs requires organic ammonium halides as the starting materials, which need to be ex situ synthesized. A novel approach to prepare high-quality RPP films through in situ formation of organic spacer cations from amines is presented. Compared with control devices fabricated from organic ammonium halides, this new approach results in similar (and even better) device performance for both solar cells and light-emitting diodes. High-quality RPP films are fabricated based on different types of amines, demonstrating the universality of the approach. This approach not only represents a new pathway to fabricate efficient devices based on RPPs, but also provides an effective method to screen new organic spacers with further improved performance.

4.
Cancer Med ; 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31397118

RESUMO

Syncytin 1 is considered as an oncogene in various malignant tumors, but its effect on non-small cell lung cancer (NSCLC) has not been reported. We investigated the specific role of Syncytin 1 on NSCLC through the transfection of Syncytin 1 knockdown or overexpression plamids in A549 cells. Our results proved that knockdown of Syncytin 1 inhibited the proliferation, and blocked the cell cycle on G1 phase by inhibiting the expression of Nusap1, Cyclin D1, CDK6, and CDK4. Cell cycle arrest also leaded to increased apoptosis in Syncytin 1 knockdown cells. Suppression of Syncytin 1 inhibited the migration and invasion, as well as the expressions of epithelial-mesenchymal transition (EMT) makers, N-cadherin, ß-catenin, and Vimentin, indicating that Syncytin 1 knockdown inhibited the metastasis via reversing the EMT process in A549 cells. The phosphorylation levels of Akt, mTOR, and Erk1/2 were all decreased in Syncytin 1 knockdown cells, suggesting the signaling pathways by which Syncytin 1 operated as an oncogene in NSCLC. Moreover, the underexpression of transcription factor SP1 downregulated the Syncytin 1 expression in A549 cells. The rescue experiment of Syncytin 1 in SP1 knockdown cells further proved that Syncytin 1 could block the inhibition of cell growth induced by SP1 knockdown. In conclusion, knockdown of SP1/Syncytin1 axis inhibited the progression of NSCLC by the reversion of tumor epithelial-mesenchymal transition process and suppression of Akt and Erk signaling pathways, suggesting that they are potential targets for targeted therapy of NSCLC.

5.
Reprod Biomed Online ; 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31444050

RESUMO

RESEARCH QUESTION: The study aimed to evaluate the associations of endocrine and ultrasound characteristics with metabolic syndrome in women with polycystic ovary syndrome (PCOS), and whether these associations were modified by body mass index (BMI). DESIGN: The study was a secondary analysis of baseline data from a randomized controlled trial of induction of ovulation in women with PCOS. RESULTS: Among 947 Chinese women with PCOS, 153 (16.2%) were diagnosed with metabolic syndrome. The prevalence of metabolic syndrome in women with normal (<24 kg/m2) and high (≥24 kg/m2) BMI was 3.6% and 30.5%, respectively. In all women, a high free androgen index (FAI ≥5%) was positively associated with metabolic syndrome (adjusted odds ratio [OR] 2.06, 95% confidence interval [CI] 1.11-3.82). High FAI was positively associated with metabolic syndrome among women with high BMI (adjusted OR 3.37, 95% CI 1.78-6.37), but the association was not significant in women with normal BMI (adjusted OR 1.27, 95% CI 0.34-4.70). The presence of polycystic ovary morphology was negatively associated with metabolic syndrome (adjusted OR 0.52, 95% CI 0.26-1.03) in all women (normal BMI adjusted OR 0.42, 95% CI 0.11-1.67; high BMI adjusted OR 0.54, 95% CI 0.23-1.28). LH, sex hormone-binding globulin (SHBG) and anti-Müllerian hormone (AMH) were negatively associated with metabolic syndrome. The associations of FAI, SHBG and AMH in relation to metabolic syndrome were significantly modified by BMI. CONCLUSION(S): The associations of endocrine characteristic with metabolic syndrome were modified by BMI in women with PCOS. Women with PCOS and normal BMI did not have an increased risk of metabolic syndrome.

6.
Surg Innov ; : 1553350619856491, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31267829

RESUMO

Background. Management of gastric cancer (GC) with liver metastases is debated. It is still controversial whether surgical resection provides a survival benefit or not. This systematic review was designed to evaluate the efficacy of hepatectomy for GC liver metastasis. Methods. We searched several electronic databases to identify eligible studies updated on September 2018. Studies assessing the efficacy and safety of hepatectomy versus no hepatectomy were included. Odds ratio (OR) along with 95% confidence interval (95% CI) were utilized for main outcome analysis. Results. In all, 10 studies were included. Patients who underwent hepatectomy had lower 1-year (OR = 0.15, 95% CI = 0.10-0.22, P < .00001), 3-year (OR = 0.16, 95% CI = 0.10-0.27, P < .00001), and 5-year mortality (OR = 0.13, 95% CI = 0.07-0.24, P < .00001) than those without hepatectomy. We also reported favorable survival outcome in patients with metachronous hepatic resection versus synchronous hepatic resection (OR = 2.09, 95% CI = 1.21-3.60, P = .008). However, there was no significant difference between solitary and multiple liver metastases (OR = 0.61, 95% CI = 0.35-1.07, P = .08). Conclusion. The present study demonstrates that hepatic resection in the management of liver metastases of GC can prolong the survival of patients and should be considered a promising treatment for such patients. Furthermore, there are more favorable outcomes in patients with metachronous metastases versus those with synchronous disease. Therefore, metachronous hepatic metastases from GC are not necessarily a contraindication for hepatectomy of the metastatic site.

7.
Nat Commun ; 10(1): 3111, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31308376

RESUMO

Biomedical repositories such as the UK Biobank provide increasing access to prospectively collected cardiac imaging, however these data are unlabeled, which creates barriers to their use in supervised machine learning. We develop a weakly supervised deep learning model for classification of aortic valve malformations using up to 4,000 unlabeled cardiac MRI sequences. Instead of requiring highly curated training data, weak supervision relies on noisy heuristics defined by domain experts to programmatically generate large-scale, imperfect training labels. For aortic valve classification, models trained with imperfect labels substantially outperform a supervised model trained on hand-labeled MRIs. In an orthogonal validation experiment using health outcomes data, our model identifies individuals with a 1.8-fold increase in risk of a major adverse cardiac event. This work formalizes a deep learning baseline for aortic valve classification and outlines a general strategy for using weak supervision to train machine learning models using unlabeled medical images at scale.

9.
Gigascience ; 8(7)2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31289832

RESUMO

BACKGROUND: The blood clam, Scapharca (Anadara) broughtonii, is an economically and ecologically important marine bivalve of the family Arcidae. Efforts to study their population genetics, breeding, cultivation, and stock enrichment have been somewhat hindered by the lack of a reference genome. Herein, we report the complete genome sequence of S. broughtonii, a first reference genome of the family Arcidae. FINDINGS: A total of 75.79 Gb clean data were generated with the Pacific Biosciences and Oxford Nanopore platforms, which represented approximately 86× coverage of the S. broughtonii genome. De novo assembly of these long reads resulted in an 884.5-Mb genome, with a contig N50 of 1.80 Mb and scaffold N50 of 45.00 Mb. Genome Hi-C scaffolding resulted in 19 chromosomes containing 99.35% of bases in the assembled genome. Genome annotation revealed that nearly half of the genome (46.1%) is composed of repeated sequences, while 24,045 protein-coding genes were predicted and 84.7% of them were annotated. CONCLUSIONS: We report here a chromosomal-level assembly of the S. broughtonii genome based on long-read sequencing and Hi-C scaffolding. The genomic data can serve as a reference for the family Arcidae and will provide a valuable resource for the scientific community and aquaculture sector.

10.
Environ Sci Technol ; 53(16): 9866-9875, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31355638

RESUMO

Animal studies have indicated that persistent organic pollutants (POPs) affect thyroid hormone homeostasis, while epidemiological studies involving human have not shown consistent results. In this study, we investigated the associations between POP exposure and thyroid function among adult population of East China. One hundred eighty-six participants diagnosed with thyroid disease and 186 participants without thyroid disease from Shandong, China were enrolled in the case-control study during 2016 to 2017. We found that POP exposure was significantly and positively associated with the risk of thyroid disease. The association of thyroid disease with a sum of 17 POPs followed a nonmonotonic dose response, with an adjusted odds ratio of 2.09 (95% confidence intervals: 1.13-3.87, p = 0.019) for the second quartile. Among 186 participants in the control group, concentrations of POPs showed negative associations with triiodothyronine (T3), free T3 (FT3), thyroxine (T4), and free T4 (FT4) in males and positive associations with T4 and FT4 in females. Taken together, these findings suggest that POP exposure can disrupt thyroid hormone homeostasis and increase the risk of thyroid disease.

11.
Environ Technol ; : 1-8, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31154957

RESUMO

As a strong oxidant, aqueous Cr(VI) possibly oxidizes the polymer resin and weakens resin adsorption property during Cr(VI) removal from solutions by ion exchange. In the present study, to minimize the resin oxidation, central composite design approach based on response surface methodology was employed with different variables (Cr(VI) concentration of 100-1000 mg L-1, temperature of 293-333 K, pH of 1-7, and reaction time of 0-120 min) using a strong anionic resin (D202). The pH and reaction time had the most significant effect on resin oxidation, followed by Cr(VI) concentration. The temperature had a comparatively less significant effect on resin oxidation. The minimal Cr(VI) reduction efficiency (0.4%) was achieved with a Cr(VI) concentration of 251.5 mg L-1, temperature of 323.1 K, pH of 6.2 and reaction time of 52.5 min. Based upon a long-term operation and Fourier transform infrared rays analyses, the oxidation pathway of D202 resin was proposed. The rupture of the C-N bond led to the disappearance of the nitrogen-containing functional groups, which resulted in a significant decrease of resin exchange capacity.

12.
J Colloid Interface Sci ; 553: 409-417, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31228755

RESUMO

Efficient multifunctional Titania/Lignocellulosic Biomass (TiO2-OP) composite photocatalysts were fabricated via ultrasonic-assisted sol-gel for the photoreduction of Cr(VI) under UV and visible light. Materials were fully characterized using FTIR, XPS, BET, SEM-EDS, UV-DRS, XRD, photo-current and contact angle measurements. It was deduced that the produced TiOC bonding bridge between TiO2 clusters and olive pits lignocellulosic surface exhibits a significant role for the visible light response and band gap narrowing, wherein, Eg values were between 3.02 and 2.63 eV as a function of TiO2/OP ratio. TiO2-OP composites showed an astonishing photocatalytic efficiency, i.e., a complete reduction of Cr(VI) at 10 ppm was found within 30 min with TiO2-OP(50%), against 90 min for TiO2 under UV light. Nevertheless, in terms of TiO2-OP(50%), a total reduction was obtained within 50 min under visible light, while no photoactivity was observed with bare-TiO2. Several plausible mechanistic pathways behind the photocatalytic efficiency of TiO2-OP composites were discussed.

13.
J Physiol ; 597(15): 3927-3950, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31206177

RESUMO

KEY POINTS: Women with polycystic ovary syndrome (PCOS) commonly suffer from miscarriage, but the underlying mechanisms remain unknown. Herein, pregnant rats chronically treated with 5α-dihydrotestosterone (DHT) and insulin exhibited hyperandrogenism and insulin resistance, as well as increased fetal loss, and these features are strikingly similar to those observed in pregnant PCOS patients. Fetal loss in our DHT+insulin-treated pregnant rats was associated with mitochondrial dysfunction, disturbed superoxide dismutase 1 and Keap1/Nrf2 antioxidant responses, over-production of reactive oxygen species (ROS) and impaired formation of the placenta. Chronic treatment of pregnant rats with DHT or insulin alone indicated that DHT triggered many of the molecular pathways leading to placental abnormalities and fetal loss, whereas insulin often exerted distinct effects on placental gene expression compared to co-treatment with DHT and insulin. Treatment of DHT+insulin-treated pregnant rats with the antioxidant N-acetylcysteine improved fetal survival but was deleterious in normal pregnant rats. Our results provide insight into the fetal loss associated with hyperandrogenism and insulin resistance in women and suggest that physiological levels of ROS are required for normal placental formation and fetal survival during pregnancy. ABSTRACT: Women with polycystic ovary syndrome (PCOS) commonly suffer from miscarriage, but the underlying mechanism of PCOS-induced fetal loss during pregnancy remains obscure and specific therapies are lacking. We used pregnant rats treated with 5α-dihydrotestosterone (DHT) and insulin to investigate the impact of hyperandrogenism and insulin resistance on fetal survival and to determine the molecular link between PCOS conditions and placental dysfunction during pregnancy. Our study shows that pregnant rats chronically treated with a combination of DHT and insulin exhibited endocrine aberrations such as hyperandrogenism and insulin resistance that are strikingly similar to those in pregnant PCOS patients. Of pathophysiological significance, DHT+insulin-treated pregnant rats had greater fetal loss and subsequently decreased litter sizes compared to normal pregnant rats. This negative effect was accompanied by impaired trophoblast differentiation, increased glycogen accumulation, and decreased angiogenesis in the placenta. Mechanistically, we report that over-production of reactive oxygen species (ROS) in the placenta, mitochondrial dysfunction, and disturbed superoxide dismutase 1 (SOD1) and Keap1/Nrf2 antioxidant responses constitute important contributors to fetal loss in DHT+insulin-treated pregnant rats. Many of the molecular pathways leading to placental abnormalities and fetal loss in DHT+insulin treatment were also seen in pregnant rats treated with DHT alone, whereas pregnant rats treated with insulin alone often exerted distinct effects on placental gene expression compared to insulin treatment in combination with DHT. We also found that treatment with the antioxidant N-acetylcysteine (NAC) improved fetal survival in DHT+insulin-treated pregnant rats, an effect related to changes in Keap1/Nrf2 and nuclear factor-κB signalling. However, NAC administration resulted in fetal loss in normal pregnant rats, most likely due to PCOS-like endocrine abnormality induced by the treatment. Our results suggest that the deleterious effects of hyperandrogenism and insulin resistance on fetal survival are related to a constellation of mitochondria-ROS-SOD1/Nrf2 changes in the placenta. Our findings also suggest that physiological levels of ROS are required for normal placental formation and fetal survival during pregnancy.

14.
Chem Commun (Camb) ; 55(56): 8166-8169, 2019 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-31241074

RESUMO

Here, based on a newly developed "Z-scheme" CdSe QD/afGQD heterojunction and multiple signal amplification strategies, a sensitive photoelectrochemical methyltransferase activity assay was carried out and a low detection limit of 0.046 U mL-1 was obtained.

15.
J Phys Chem Lett ; 10(11): 3171-3175, 2019 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-31119942

RESUMO

Two-dimensional (2D) perovskites incorporating hydrophobic organic spacer cations show improved film stability and morphology compared to their three-dimensional (3D) counterparts. However, 2D perovskites usually exhibit low photoluminescence quantum efficiency (PLQE) owing to strong exciton-phonon interaction at room temperature, which limits their efficiency in light-emitting diodes (LEDs). Here, we demonstrate that the device performance of 2D perovskite LEDs can be significantly enhanced by doping Mn2+ in (benzimidazolium)2PbI4 2D perovskite films to suppress the exciton-phonon interaction. The distorted [PbI6]4- octahedra by Mn-doping and the rigid benzimidazolium (BIZ) ring without branched chains in the 2D perovskite structure lead to improved crystallinity and rigidity of the perovskites, resulting in suppressed phonon-exciton interaction and enhanced PLQE. On the basis of this strategy, for the first time, we report yellow electroluminescence from free excitons in 2D ( n = 1) perovskites with a maximum brightness of 225 cd m-2 and a peak EQE of 0.045%.

16.
Nanoscale ; 11(22): 10702-10708, 2019 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-31140521

RESUMO

Glucose metabolism plays an important role in cell energy supply, and quantitative detection of the intracellular glucose level is particularly important for understanding many physiological processes. Glucose electrochemical sensors are widely used for blood and extracellular glucose detection. However, intracellular glucose detection cannot be achieved by these sensors owing to their large size and consequent low spatial resolution. Herein, we developed a single nanowire glucose sensor for electrochemical detection of intracellular glucose by depositing Pt nanoparticles (Pt NPs) on a SiC@C nanowire and further immobilizing glucose oxidase (GOD) thereon. Glucose was converted by GOD to an electroactive product H2O2 which was further electro-catalyzed by Pt NPs. The glucose nanowire sensor is endowed with a high sensitivity, high spatial-temporal resolution and enzyme specificity due to its nanoscale size and enzymatic reaction. This allows the real-time monitoring of the intracellular glucose level, and the increase of the intracellular glucose level induced by a novel potential hypoglycemic agent, reinforcing its potential application in lowering the blood glucose level. This work provides a versatile method for the construction of enzyme-modified nanosensors to electrochemically detect intracellular non-electroactive molecules, which is of great benefit for physiological and pathological studies.

17.
J Cell Biochem ; 120(10): 17500-17511, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31111536

RESUMO

Osteoarthritis (OA) is the utmost commonly arising joint disease. Knee condyles play an essential role during OA progression. Circular RNA (or circRNA) is a novel kind of RNA, which, unlike the well-known linear RNA, plays an important regulatory role in OA on the basis of a previous research. In our study, expression of circRNAs in OA knee condyle was measured by illumine sequencing platform. A total of 197 differentially expressed circRNAs, such as hg38_circ_0007474 and hg38_circ_0000118 were identified, and 21 target miRNAs, 2466 source genes and 166 394 circRNA-miRNA-mRNA pairs were predicted. Further analysis was applied on three OA-related circRNAs (hsa_circ_0045714, hsa_circ_0002485, and hsa_circ_0005567). The results were partly verified by previous studies. Further biological research is needed to unfold the possible pathway and therapeutic target of OA.

18.
J Hazard Mater ; 374: 167-176, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30999140

RESUMO

A novel CuO-CeO2-CoOx nanocatalyst was prepared for heterogeneous Fenton-like reaction to break the Ni2+-chelate into free Ni2+ in electroless nickel plating wastewater, followed by separation of Ni2+ in an insoluble form. The composite nanocatalysts prepared by co-precipitation method were characterized by XRD, TEM, and XPS, et al. Its catalytic activity as Fenton-like reagent was evaluated by the removal efficiency of Ni(II)-citrate after decomplexation and postprecipitation treatment. Subsequently, the effects of operating parameters on the decomplexation efficiency of the nanocatalysts were investigated including calcination temperature of catalysts, H2O2 concentration, catalyst dosage, initial pH and reaction temperature. Under optimized condition, the Ni(II)-citrate complexes (C0 = 1.0 mM) achieved the complete decomplexation (>99.9%) within 60-min reaction using 0.3 g/L of CuO-CeO2-CoOx calcined at 450 °C and 75 mM of H2O2 at pH 3.0 under 50 °C of reaction. Then, Ni2+ after decomplexation could be completely removed by the subsequent precipitation at pH 11.0. In addition, the life test of CuO-CeO2-CoOx catalyst indicated that, after recycled 10 times, its activity for decomplexation of Ni(II)-citrate decreased no more than 8%. As a result, this new heterogeneous Fenton-like process is promising for decomplexation and purification of electroless nickel plating wastewater as a sludge reduction technology.

19.
Autoimmunity ; 52(1): 21-26, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30822156

RESUMO

OBJECTIVES: Recent evidence has demonstrated that UBASH3A play a pivotal role in multiple autoimmune diseases. In this study, we explored the association between UBASH3A gene single-nucleotide polymorphisms (SNPs) and rheumatoid arthritis (RA) in a Chinese Han population. We also comparatively evaluated the UBASH3A expression profile in peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls. METHODS: Four UBASH3A polymorphisms (rs1893592, rs11203203, rs2277798, and rs3788013) were studied in 553 patients with RA and 587 controls in a Chinese population. Genotyping was performed using the Fluidigm 192.24 Dynamic Array Integrated Fluidic Circuit (IFC). For gene expression study, UBASH3A mRNA levels of 30 RA patients and 31 healthy individuals were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). Data were analyzed by SPSS 19.0 software. RESULTS: A significant association between rs1893592 polymorphism and RA was found under all genetic models (all p<.05). We also discovered a significant association between rs3788013 polymorphism and RA in the allele and genotype distributions, as well as the recessive model (all p<.05). Moreover, we found the genotype distribution and allele frequency of rs1893592 were significantly associated with RF phenotype in the RA patients (χ2 = 6.786, p=.034; χ2 = 4.534, p=.033; respectively). We also found the genotype distribution and allele frequency of rs2277798 were significantly associated with anti-CCP phenotype in the RA patients (χ2 = 7.873, p=.020; χ2 = 4.473, p=.034; respectively). However, we did not detect any significant associations between rs11203203 and RA susceptibility and autoantibody profiles (all p>.05). The mRNA expression of UBASH3A was increased in PBMCs of patients with RA when compared to healthy controls (p=.001). CONCLUSIONS: Our observations suggested that the dysregulation of UBASH3A might be associated with the pathogenesis of RA, and UBASH3A gene polymorphisms (rs1893592 and rs3788013) might contribute to RA susceptibility in Chinese Han population.

20.
Environ Pollut ; 249: 381-389, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30913437

RESUMO

Annual air samples were collected at various sites in the Fildes Peninsula, West Antarctica from December 2010 to January 2018 using XAD-2 resin passive air samplers to investigate concentrations, temporal trends and potential sources of persistent organic pollutants (POPs) in Antarctic air. Relatively low concentrations of polychlorinated biphenyls (PCBs) (Σ19PCBs: 1.5-29.7 pg/m3), polybrominated diphenyl ethers (PBDEs) (Σ12PBDEs: 0.2-2.9 pg/m3) and organochlorine pesticides (OCPs) (Σ13OCPs: 101-278 pg/m3) were found in the atmosphere of West Antarctica. PCB-11, BDE-47 and hexachlorobenzene (HCB) were the predominant compounds in the atmosphere. The concentrations of PCBs, HCHs, DDTs and endosulfans were found to show decreasing temporal trends, whereas uniform temporal trends were observed for HCB. The atmospheric half-life values for PCBs, HCHs, DDTs and endosulfans in Antarctic air were estimated for the first time, using regressions of the natural logarithm of the concentrations versus the number of years, obtaining the values of 2.0, 2.0, 2.4 and 1.2 year, respectively. An increasing ratio of α-HCH/γ-HCH indicated long residence time for α-HCH and possible transformation of γ-HCH to α-HCH in the atmosphere. The ratios of p,p'-DDT/p,p'-DDE were mostly lower than unity in this study, which could be attributed to aged sources. It was found that long-range atmospheric transport was still considered to be the main contributing factor to the atmospheric levels of the POPs in West Antarctica whereas the contribution of human activities at the Chinese Great Wall Station was minor. The results of this study give a view on the most recent temporal trends and provide new insights regarding the occurrence of various POPs in the Antarctic atmosphere.


Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Éteres Difenil Halogenados/análise , Hidrocarbonetos Clorados/análise , Praguicidas/análise , Bifenilos Policlorados/análise , Regiões Antárticas , Atmosfera , Endossulfano/análise , Hexaclorobenzeno/análise , Hexaclorocicloexano/análise , Humanos
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