Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Shanghai Kou Qiang Yi Xue ; 28(4): 397-401, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31792481

RESUMO

PURPOSE: To assess the prevalence of fenestration and dehiscence of anterior teeth in adolescent patients with unilateral cleft lip and palate(UCLP). METHODS: Twenty-five males and seventeen females with UCLP who visited The Second Xiangya Hospital from June 2014 to September 2017 were selected. Forty-two age- and sex- matched non-cleft skeletal III patients were enrolled as the control group. Cone-beam CT(CBCT) scans were evaluated to detect fenestration and dehiscence.The data were analyzed using SPSS 22.0 software package. RESULTS: The prevalence of dehiscence of anterior teeth was 50.88% on the cleft side,42.39% on the non-cleft side and 28.77% on the controls, respectively. Dehiscence of maxillary teeth was more common on cleft side than the controls (P<0.05), and dehiscence of maxillary lateral incisor was common on non-cleft side compared with the control (P<0.05). Maxillary central incisor on the cleft side showed a higher prevalence of dehiscence compared with the non-cleft side (P<0.05). Most of the dehiscence was detected on the buccal side. Fenestration of the teeth showed no significant difference among the cleft side, non-cleft side and the control (P>0.05). CONCLUSIONS: Adolescent patients with UCLP show a high prevalence of fenestration and dehiscence,which deserves to be taken into consideration during clinical treatment.


Assuntos
Fenda Labial , Fissura Palatina , Adolescente , Tomografia Computadorizada de Feixe Cônico , Feminino , Humanos , Masculino , Prevalência
2.
Shanghai Kou Qiang Yi Xue ; 28(2): 136-140, 2019.
Artigo em Chinês | MEDLINE | ID: mdl-31384897

RESUMO

PURPOSE: To compare enamel surface state and operating time following debonding by using 5 different resin removal methods. METHODS: Forty healthy human premolars extracted for orthodontic purpose were selected and then divided into 5 groups randomly based on different resin-removal methods, 8 in each group. After regular bonding brackets and debonding, tungsten carbide bur (group A), tungsten carbide bur+silicon particles(group B), corundum bur(group C), corundum bur+silicon particles (group D) and silicon particles (group E) were used respectively to deal with the enamel surfaces. The operating time(T) of each group was recorded. The samples were observed under scanning electron microscope (SEM), and enamel damage index(EDI) was recorded. The scores of EDI were analysed using SPSS 20.0 software package . RESULTS: The order of EDI was group C>group D>group A >group B>group E, significant difference was found between 5 groups (P<0.05). The order of operating time was group E>group D>group B>group A>group C, significant difference was found between 5 groups (P<0.05). CONCLUSIONS: Using tungsten carbide bur followed by silicon particles to remove residual resin on enamel surface causes less damage to the enamel surface, and requires reasonable time.


Assuntos
Colagem Dentária , Descolagem Dentária , Braquetes Ortodônticos , Resinas Acrílicas , Dente Pré-Molar , Esmalte Dentário , Humanos , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Propriedades de Superfície
3.
Huan Jing Ke Xue ; 38(11): 4580-4588, 2017 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965401

RESUMO

The flooding-drying cycles in the water-level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR) result in an abundance of plants that are submerged and decomposed to release nutrients. This has the potential to lead to water quality deterioration of the TGR. Cynodon dactylon (Linn.) Pers., one of the typical plants in the WLFZ, was collected and inundated in the laboratory under different conditions under temperatures of 15℃ and 25℃, a pH 5.0 to 9.0, and under light and dark conditions, respectively, to measure the decomposition rate and nitrogen and phosphorus release mechanisms. The dry weight lost with the contents of nitrogen and phosphorus was found to decrease after Cynodon dactylon (Linn.) Pers. was soaked for decomposition. The amount released of total nitrogen (TN) and total phosphorus (TP) at 25℃ for 200-days inundation was(2.66±0.29)g·kg-1 or(3.76±0.04)g·kg-1, respectively and at 15℃ was(0.79±0.03) g·kg-1 or (1.40±0.02) g·kg-1, respectively. When the pH of the water submerging the grass was 5.0, 7.0 and 9.0, the amount of TN released was (3.76±0.08) g·kg-1, (2.66±0.29) g·kg-1, and (2.55±0.12) g·kg-1, respectively while the amount of TP released was (1.53±0.04) g·kg-1, (0.79±0.03) g·kg-1, and (1.70±0.07) g·kg-1, respectively. The TN and TP released was (3.87±0.14) g·kg-1 and (1.78±0.08) g·kg-1 under dark condition. The lower the temperature, the higher the amount of TN and TP will be released for inundation from Cynodon dactylon (Linn.) Pers. When the overlying water is acidic or alkaline, more TN and TP is released. Dark conditions are beneficial to nitrogen and phosphorus release into the overlying water. Thus, the water environment changes in each flooding season in winter. Sewage discharge will also accelerate the nutrients released from soaked plants through their decomposition in the WLFZ, and then will aggravate the deterioration of water quality in TGR.


Assuntos
Cynodon/metabolismo , Água Doce/análise , Nitrogênio/metabolismo , Fósforo/metabolismo , Poluentes Químicos da Água/metabolismo , China , Inundações , Qualidade da Água
4.
Oncotarget ; 7(10): 11733-43, 2016 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-26887056

RESUMO

Our earlier findings indicate that the long non-coding RNA MALAT1 promotes colorectal cancer (CRC) cell proliferation, invasion and metastasis in vitro and in vivo by increasing expression of AKAP-9. In the present study, we investigated the molecular mechanism by which MALAT1 enhances AKAP9 expression in CRC SW480 cells. We found that MALAT1 interacts with both SRPK1 and SRSF1. MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation. Following MALAT1 knockdown, overexpression of SRPK1 was sufficient to restore SRSF1 phosphorylation and AKAP-9 expression to a level that promoted cell proliferation, invasion and migration in vitro. Conversely, SRPK1 knockdown after overexpression of MALAT1 in SW480 cells diminished SRSF1 phosphorylation and AKAP-9 expression and suppressed cell proliferation, invasion and migration in vitro. These findings suggest MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation in CRC cells. These results reveal a novel molecular mechanism by which MALAT1 regulates AKAP-9 expression in CRC cells.


Assuntos
Proteínas de Ancoragem à Quinase A/metabolismo , Neoplasias Colorretais/metabolismo , Proteínas do Citoesqueleto/metabolismo , RNA Longo não Codificante/metabolismo , Fatores de Processamento de Serina-Arginina/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Humanos , Fosforilação , RNA Longo não Codificante/genética , Fatores de Processamento de Serina-Arginina/genética , Transfecção
5.
Biochim Biophys Acta ; 1852(9): 1876-86, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26071641

RESUMO

N-myc downstream-regulated gene 1 (NDRG1) has been implicated in tumorigenesis and metastasis in different cancers. However, its role in nasopharyngeal carcinoma remains unknown. We found that NDRG1 expression level was high in nasopharyngeal cancer 5-8F cells but low in 5-8F-LN cells with lymphatic metastasis potential. Knockdown of NDRG1 by shRNA promoted 5-8F cell proliferation, migration, and invasion in vitro and its tumorigenesis in vivo. Moreover, NDRG1 deficiency induced an epithelial-mesenchymal transition (EMT) of 5-8F cells as shown by an attenuation of E-cadherin and an induction of N-cadherin and vimentin expression. NDRG1 knockdown also enhanced Smad2 expression and phosphorylation. Smad2 signaling was attenuated in 5-8F cells but was significantly activated in 5-8F-LN cells. Knockdown of Smad2 restored E-cadherin but attenuated N-cadherin expression in NDRG1-deficient 5-8F cells, suggesting a reduction of EMT. Consistently, blockade of Smad2 in 5-8F-LN cells increased E-cadherin while diminishing N-cadherin and vimentin expression. These data indicate that Smad2 mediates the NDRG1 deficiency-induced EMT of 5-8F cells. In tumors derived from NDRG1-deficient 5-8F cells, E-cadherin expression was inhibited while vimentin and Smad2 were increased in a large number of cancer cells. Most importantly, NDRG1 expression was attenuated in human nasopharyngeal carcinoma tissues, resulted in a lower survival rate in patients. The NDRG1 was further decreased in the detached nasopharyngeal cancer cells, which was associated with a further reduced survival rate in patients with lymphatic metastasis. Taken together, these results demonstrated that NDRG1 prevents nasopharyngeal tumorigenesis and metastasis via inhibiting Smad2-mediated EMT of nasopharyngeal cells.

6.
Int J Clin Exp Pathol ; 7(11): 7451-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25550780

RESUMO

OBJECTIVE: This study aims to explore the function of Integrin-ß/FAK in the mechanical signal transduction and the connection with downstream ERK signal pathways. METHODS: Human osteosarcoma MG63 cell lines were used in this study. The effects of mechanical strain on the Integrin-ß1 expression, FAK and ERK signal pathway in Human osteosarcoma MG63 cells were detected using RT-PCR and Western-blotting methods. The localization of FAK in Human osteosarcoma MG63 cells were determined using immunofluorescent method. The interaction between Integrin-ß1 and FAK were detected by using co-immunoprecipitation method. RESULTS: The expression of Integrin-ß1 shows a notable bimodel distribution, mechanical strain stimulation can promote Integrin-ß1 expression and the phosphorylation of FAK and ERK, mechanical strain activated FAK and ERK mediated by Integrin-ß1. CONCLUSION: Integrin-ß1 may play an important role in osteoblast proliferation differentiation process, it might feel external strain stimulation through ECM composition and makes FAK phosphated through the interaction with FAK, thus causing a series of activation of signal molecules. Finally it reduces MAPK (ERK) activation and cellular responses to finish mechanical signal transduction.


Assuntos
Quinase 1 de Adesão Focal/metabolismo , Integrina beta1/metabolismo , Sistema de Sinalização das MAP Quinases , Diferenciação Celular , Linhagem Celular Tumoral , Proliferação de Células , Quinase 1 de Adesão Focal/genética , Humanos , Integrina beta1/genética , Fosforilação
7.
Acta Crystallogr Sect E Struct Rep Online ; 68(Pt 2): m187, 2012 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-22346858

RESUMO

The water-coordinated Ni(2+) cation in the title compound, [Ni(C(10)H(5)N(3)O(4))(H(2)O)](n), assumes an octa-hedral NiN(3)O(3) coord-ination mode and is N,O-chelated by two deprotonated 2-(pyridin-4-yl)-1H-imidazole-4,5-dicarb-oxy-lic acid (HPyImDC(2-)) ligands, forming a layer structure extending in the bc plane. The chains are arranged along the b-axis direction, forming a layer structure extending in the bc plane. O-H⋯O hydrogen bonding between the layers results in the formation of a three-dimensional supra-molecular framework. The structure is isotypic with the Zn analogue [Li et al. (2009). Cryst. Growth Des.6, 3423-3431].

8.
Cell Physiol Biochem ; 28(2): 297-304, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21865737

RESUMO

To explore the possible role for connective tissue growth factor (CTGF) during tooth movement, we evaluated CTGF gene and protein expression in MG-63 cells subjected to cyclic stretch. Cyclic stretch caused a time-dependent increase in CTGF mRNA and protein levels.Inhibition of p38 MAP kinase or ERK activation did not affect cyclic stretch-induced CTGF expression. Specific inhibitors of PI3K suppressed stretch -induced CTGF expression in a time-dependent manner. cyclic stretch activated JNK and ERK, but not p38 MAP kinase in osteoblast-like cells. PI3K inhibitors suppressed cyclic stretch-induced JNK, but not p38 MAP kinase activation. Finally, SP600125, a Specific Inhibitor of JNK, suppressed stretch -induced CTGF Expression. These results suggest that stretch-induced CTGF expression is mediated through the PI3K-JNK -dependent pathway, not by p38 MAP kinase and ERK pathways.


Assuntos
Fator de Crescimento do Tecido Conjuntivo/metabolismo , Osteoblastos/metabolismo , Transdução de Sinais , Estresse Mecânico , Antracenos/farmacologia , Linhagem Celular , Cromonas/farmacologia , Fator de Crescimento do Tecido Conjuntivo/genética , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Flavonoides/farmacologia , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Morfolinas/farmacologia , Osteoblastos/efeitos dos fármacos , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
9.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 25(5): 497-9, 503, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18072570

RESUMO

OBJECTIVE: To explore certain principle of how osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) take part in the periodontal tissues remodeling under the combined influence of inflammation and orthodontic force. METHODS: The positive signals of OPG and OPGL mRNA were measured with in situ hybridzation after orthodontic tooth movement in the experimental periodontitis groups and control ones. RESULTS: The OPG and OPGL mRNA expression intensity in the experimental group showed difference from control. All their optical density index reached a peak in day 2, respectively. CONCLUSION: OPG and OPGL play important roles in the periodontal reconstruction induced by inflammation irritation and orthodontic force, and complex interaction could exist between the two factors.


Assuntos
Osteoprotegerina , Ligante RANK , Humanos , Periodontite , RNA Mensageiro , Técnicas de Movimentação Dentária
10.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 24(2): 106-9, 2006 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16704083

RESUMO

OBJECTIVE: To study the integrin beta3 mRNA changes after orthodontic treatment on normal teeth and periodontitis teeth in rats. METHODS: 96 adult SD rats of 10 weeks old were randomly divided into normal tooth move-ment group and periodontitis tooth movement group. The rats in the two groups were sacrificed after 0 d, 12 h, 1 d, 3 d, 5 d and 7 d of tooth movement. The alveolar specimens were prepared. The integrin beta3 mRNA were detected using in situ hybridization in the specimens. The OD index of positively stained osteoclasts for integrin beta3 mRNA after orthodontic tooth movement in the two groups were measured and compared. RESULTS: There were weak positive signals on the cytoplasm of osteoclasts in periodontum in both groups after 12 hours and 3 days force activation. No positive signals were detected in the rest samples. There was no difference in the OD of positive stained osteoclasts between normal and periodontitis groups. Strong expressions were present on cells with one or two nuclei in the alveolar marrow. CONCLUSION: It is suggested that integrin beta3 mRNA is related with osteoclasts maturation and migration in orthodontic tooth movement.


Assuntos
RNA Mensageiro , Ratos Sprague-Dawley , Animais , Hibridização In Situ , Integrinas , Osteoclastos , Periodontite , Ratos , Técnicas de Movimentação Dentária
11.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 23(2): 161-3, 2005 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-15952634

RESUMO

OBJECTIVE: To study the integrin beta1 mRNA changes after orthodontic tooth movement in normal teeth and periodontitis teeth of rats. METHODS: The OD of positively stained osteoclasts for integrin beta1 mRNA using in situ hybridzation was detected after orthodontic tooth movement in normal teeth and periodontitis teeth groups. RESULTS: Integrin beta1 mRNA expression were detected on all osteoclasts in tooth movement samples of normal and periodontitis teeth. There were stronger positive signals after given orthodontic force in both of the two groups. But no differences were found after 0.5, 1, 2, 3, 5, 7, 10 days since orthodontic tooth movement. The integrin beta1 mRNA signals in normal tooth movement group were not different from that in periodontitis group. CONCLUSION: The integrin beta1 of osteoclasts may play a role in the stability and remodeling of periodontal ligament in orthodontic tooth movement. There were no difference in the OD of integrin beta1 mRNA staining in orthodontic tooth movement between normal teeth group and periodontitis teeth group.


Assuntos
Integrina beta1/metabolismo , Periodontite/fisiopatologia , Técnicas de Movimentação Dentária , Animais , Osteoclastos , Ligamento Periodontal , RNA Mensageiro/metabolismo , Ratos
12.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 23(1): 32-4, 2005 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15804016

RESUMO

OBJECTIVE: To study the bracket placement and arch wire bending based on ethnic differences and individual differences of normal occlusion. METHODS: The prominence, tip, torque, upper first molar offset of crown and arch form between Chinese and Caucasian normal occlusion were compared. RESULTS: The results showed the ethnic differences of prominence, tip, torque, upper first molar offset of crown and arch form between Chinese and Caucasian normal occlusion. The placement of bracket was influenced by the crown morphology. CONCLUSION: The adjustments of the bracket placement and arch wire bending with Edgewise and pre-adjusted appliance are necessary to adapt to ethnic difference and individual difference.


Assuntos
Oclusão Dentária , Dente Molar , Humanos , Aparelhos Ortodônticos
13.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 38(3): 233-5, 2003 May.
Artigo em Chinês | MEDLINE | ID: mdl-12887808

RESUMO

OBJECTIVE: To evaluate the bracket bond failure and its causes between adult and adolescent patients during fixed orthodontic therapy. METHODS: Bracket bond failure data of 30 adults and 30 adolescents, receiving fixed orthodontic therapy, have been collected within the first 12 visits, respectively. The compliance has been analyzed with survival analyse between the two groups. RESULT: The general bracket bond failure rate in the adult group is lower than that of the adolescent group and the difference is significant (p < 0.05). In the adolescent group, the failure rate for mandibular anterior teeth is highest and different from that of the adult group (p < 0.05). The failure rate resulted from biting hard food is ranked No. 1. CONCLUSIONS: The compliance of the adults receiving fixed orthodontic therapy is better than that of the adolescents. The investigation of bracket bond failure causes is beneficial in helping orthodontists improve orthodontic practice and raise clinical efficiency. The survival analysis is effective in evaluating the bond failure.


Assuntos
Braquetes Ortodônticos , Ortodontia Corretiva , Adolescente , Adulto , Falha de Equipamento , Feminino , Humanos , Masculino , Análise de Sobrevida
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...