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1.
Rev. bras. med. esporte ; 28(5): 486-488, Set.-Oct. 2022. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376690

RESUMO

ABSTRACT Introduction: Obesity is a major cause of chronic diseases such as cardiovascular and cerebrovascular diseases, cancer, and diabetes. Recent studies have reported that combined aerobic and anaerobic exercise effects are more effective for fat reduction. However, aerobic and anaerobic exercise have different fat reduction mechanisms. Comparing the intervention effects of different combinations of these exercises could provide an experimental basis for establishing an optimal protocol for weight loss. Objective: To study the effect of different aerobic exercise intensities on cardiorespiratory endurance in obese patients and its impact on fat loss. Methods: 18 obese female college students (BMI≥25) were randomly divided into two groups with different proportions of aerobic and anaerobic exercise. Bioimpedance data and BMI were collected for analysis. SPSS 25.0 software was used for statistics, with mean and standard deviation expressed for each index. Significance level at p<0.05 and highly significant set at P<0.01. Results: The weight, BMI, waist-to-hip ratio, and body fat content of the two exercise methods were positively changed before and after the experiment. This change had a very significant difference. Conclusion: There was no statistical difference in the interventions for the six weeks. Aerobic exercise can effectively improve cardiopulmonary function, and the benefits are directly proportional to the period practiced. Evidence Level II; Therapeutic Studies - Investigating the results.


RESUMO Introdução: A obesidade é uma causa importante de doenças crônicas como doenças cardiovasculares e cerebrovasculares, câncer e diabetes. Estudos recentes relataram que os efeitos do exercício aeróbico e anaeróbico combinados são mais eficazes para a redução de gordura. O exercício aeróbico e o exercício anaeróbico têm diferentes mecanismos de redução de gordura e a comparação dos efeitos da intervenção de diferentes combinações desses exercícios poderia fornecer uma base experimental para o estabelecimento de um protocolo ideal para perda de peso. Objetivo: Estudar o efeito de diferentes intensidades de exercícios aeróbicos sobre a resistência cardiorrespiratória de pacientes obesos e seu impacto sobre a perda de gordura. Métodos: 18 estudantes universitárias obesas (IMC≥25) foram divididas aleatoriamente em dois grupos com proporções distintas de exercícios aeróbicos e anaeróbicos. Assim como o IMC, dados coletados via bioimpedância foram mensurados antes e depois do experimento para análise. Foi utilizado o Software SPSS 25.0 para a estatística, com média e desvio padrão expressos para cada índice. Nível de significância em p<0,05 e muito significativo fixado em P<0,01. Resultados: O peso, IMC, relação cintura/quadril, e conteúdo de gordura corporal dos dois métodos de exercício foram positivamente alterados antes e depois do experimento. Essa alteração teve uma diferença muito significativa. Conclusão: Não houve diferença estatística nas intervenções pelo período de seis semanas. O exercício aeróbico pode efetivamente melhorar a função cardiopulmonar e os benefícios são diretamente proporcionais ao período praticado. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: La obesidad es una de las principales causas de enfermedades crónicas como las cardiovasculares y cerebrovasculares, el cáncer y la diabetes. Estudios recientes han informado que los efectos combinados de los ejercicios aeróbicos y anaeróbicos son más eficaces para la reducción de la grasa. Sin embargo, el ejercicio aeróbico y el anaeróbico tienen diferentes mecanismos de reducción de la grasa. La comparación de los efectos de intervención de diferentes combinaciones de estos ejercicios podría proporcionar una base experimental para establecer un protocolo óptimo para la pérdida de peso. Objetivo: Estudiar el efecto de diferentes intensidades de ejercicio aeróbico sobre la resistencia cardiorrespiratoria en pacientes obesas y su impacto en la pérdida de grasa. Métodos: 18 estudiantes universitarias obesas (BMI≥25) fueron divididas aleatoriamente en dos grupos con diferentes proporciones de ejercicio aeróbico y anaeróbico. Se recogieron los datos de bioimpedancia y el IMC para su análisis. Se utilizó el software SPSS 25.0 para las estadísticas, con el promedio y la desviación estándar expresadas para cada índice. El nivel de significancia se fijó en p<0,05 y el nivel muy significativo en P<0,01. Resultados: El peso, el IMC, la relación cintura-cadera y el contenido de grasa corporal de los dos métodos de ejercicio cambiaron positivamente antes y después del experimento. Este cambio tuvo una diferencia muy significativa. Conclusión: No hubo diferencias estadísticas en las intervenciones durante las seis semanas. El ejercicio aeróbico puede mejorar eficazmente la función cardiopulmonar, y los beneficios son directamente proporcionales al periodo practicado. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

2.
JAMA Netw Open ; 5(8): e2225991, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35947383

RESUMO

Importance: The landscape of tobacco use is changing. However, information about the association between early-age tobacco use and cognitive performances is limited, especially for emerging tobacco products such as electronic cigarettes (e-cigarettes). Objective: To assess the association between early-age initiation of tobacco use and cognitive performances measured by the National Institutes of Health (NIH) Toolbox Cognitive Battery and to examine whether initiation is associated with differences in brain morphometry. Design, Setting, and Participants: This observational cohort study examined the longitudinal associations of initiation of tobacco use with neurocognition using multivariate linear mixed models. Children aged 9 to 10 years from 21 US sites were enrolled in wave 1 (October 1, 2016, to October 31, 2018 [n = 11 729]) and the 2-year follow-up (August 1, 2018, to January 31, 2021 [n = 10 081]) of the Adolescent Brain Cognitive Development (ABCD) Study. Exposures: Ever use (vs none) of any tobacco products at wave 1, including e-cigarettes, cigarettes, cigars, smokeless tobacco, hookah, pipes, and nicotine replacement. Main Outcomes and Measures: Neurocognition measured by the NIH Toolbox Cognition Battery and morphometric measures of brain structure and region of interest analysis for the cortex from structural magnetic resonance imaging. Results: Among 11 729 participants at wave 1 (mean [SE] age, 9.9 [0.6] years; 47.9% girls and 52.1% boys; 20.3% Hispanic; 14.9% non-Hispanic Black; and 52.1% non-Hispanic White), 116 children reported ever use of tobacco products. Controlling for confounders, tobacco ever users vs nonusers exhibited lower scores in the Picture Vocabulary Tests at wave 1 (b [SE] = -2.9 [0.6]; P < .001) and 2-year follow-up (b [SE] = -3.0 [0.7]; P < .001). The crystalized cognition composite score was lower among tobacco ever users than nonusers both at wave 1 (b [SE] = -2.4 [0.5]; P < .001) and 2-year follow-up (b [SE] = -2.7 [0.8]; P = .005). In structural magnetic resonance imaging, the whole-brain measures in cortical area and volume were significantly lower among tobacco users than nonusers, including cortical area (b [SE] = -5014.8 [1739.8] mm2; P = .004) at wave 1 and cortical volume at wave 1 (b [SE] = -174 621.0 [5857.7] mm3; P = .003) and follow-up (b [SE] = -21 790.8 [7043.9] mm3; P = .002). Further region of interest analysis revealed smaller cortical area and volume in multiple regions across frontal, parietal, and temporal lobes at both waves. Conclusions and Relevance: In this cohort study, initiating tobacco use in late childhood was associated with inferior cognitive performance and reduced brain structure with sustained effects at 2-year follow-up. These findings suggest that youths vulnerable to e-cigarettes and tobacco products should be treated as a priority population in tobacco prevention.

3.
J Cancer ; 13(9): 2872-2883, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35912012

RESUMO

Purpose: Liver kinase B1 (LKB1), also known as serine/threonine kinase 11, was considered as a tumor suppressor, which exhibited anti-cancer activity in a variety of cancers. However, the effect of LKB1 in thyroid cancer remains unclear. Methods: In the study, MTT assay, colony formation assay, flow cytometry, western blot analysis, wound healing assay, transwell assays, quantitative real-time PCR, HUVEC migration assay, ELISA assay, tube formation assay and nude mice xenograft were used to investigate the anti-cancer capacity of LKB1 in thyroid cancer in vitro and in vivo. Results: In the present study, we found that the expression of LKB1 was lower in thyroid cancer tissues and cell lines, compared with the adjacent normal tissue and thyroid epithelial cell. After construction of stable clone cells with ectopic LKB1 overexpression, the findings revealed that LKB1 overexpression exerted anti-proliferative and pro-apoptotic property in thyroid cancer TPC-1 and BCPAP cells. In addition, LKB1 overexpression could inhibit migration and invasion, downregulate MMP2 and MMP9 expressions, and reverse EMT in thyroid cancer cells. Furthermore, overexpression of LKB1 attenuated HUVEC recruitment, decreased the expression of VEGFA and inhibited the formation of new vessels in thyroid cancer cells. To validate the underlying mechanism of LKB1 in thyroid cancer, the results showed that LKB1 could positively regulate SIK1 in thyroid cancer TPC-1 and BCPAP cells. Additionally, the SIK1 inhibitor HG-9-91-01 could partially abrogate the anti-proliferative and anti-metastatic effect of LKB1, and reverse MET (mesenchymal-to-epithelial transition) mediated by LKB1 overexpression. Ultimately, the results in vivo revealed that LKB1 overexpression exhibited a strong inhibitory effect of tumorigenicity and presented anti-angiogenic characteristic in nude mice xenograft model. Conclusion: the results demonstrated that LKB1 could inhibit proliferation, metastasis phenotype and angiogenesis, and reverse EMT in thyroid cancer in vitro and vivo via the upregulation of SIK1, suggesting that LKB1 could be considered as a potential therapeutic target for the treatment of thyroid cancer.

4.
J Mater Chem B ; 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35916308

RESUMO

Microneedles (MNs) have been developed for various applications such as drug delivery, cosmetics, diagnosis, and biosensing. To meet the requirements of MNs used in these areas, numerous materials have been used for the fabrication of MNs. However, MNs will be exposed to skin tissues after piercing the stratum corneum barrier. Thus, it is necessary to ensure that the matrix materials of MNs have the characteristics of low toxicity, good biocompatibility, biodegradability, and sufficient mechanical properties for clinical application. In this review, the matrix materials currently used for preparing MNs are summarized and reviewed in terms of these factors. In addition, MN products used on the market and their applications are summarized in the end. This work may provide some basic information to researchers in the selection of MN matrix materials and in developing new materials.

5.
Int J Ophthalmol ; 15(7): 1142-1148, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35919312

RESUMO

AIM: To evaluate temporal retinal thinning changes in retinal layers using spectral-domain optical coherence tomography (SD-OCT) in pediatric X-linked Alport syndrome (XLAS) patients. METHODS: A retrospective case-control study. SD-OCT scans of pediatric patients diagnosed with XLAS and age- and sex-matched healthy control participants were reviewed. Automated segmentation of SD-OCT scans was induced to analyze the retinal thickness (RT) of different layers. The temporal thinning index (TTI) was calculated for each layer and compared between the patients and the control group. RESULTS: Forty-three pediatric XLAS patients and 60 healthy controls were included. Temporal retinal thinning was present in 33 patients (76.74%), while 28 patients (65.11%) had severe pathological temporal retinal thinning and 5 patients (11.63%) had moderate thinning. The temporal inner sector RT (P<0.0001), the temporal outer sector RT (P<0.0001), and the nasal outer sector RT (P=0.0211) were significantly thinner in the XLAS male patients. The TTI of the total retina was significantly higher in the XLAS group than in the control group (P<0.0001). The TTI of the inner retina layers (P<0.0001), ganglion cell layer (P<0.0001), inner plexiform layer (P<0.0001), inner nuclear layer (P<0.0001), and outer nuclear layer (P<0.0001) were significantly higher in the XLAS group. The central RT of the XLAS group was significantly thinner than that of the control group (P<0.0001). CONCLUSION: Temporal retinal thinning appears early in XLAS patients, especially in male patients. The thinning is mainly caused by structural abnormalities of the inner retina. This suggests that temporal retinal thinning could be helpful for the early diagnosis and follow-up of XLAS with noninvasive SD-OCT examination.

6.
Adv Mater ; : e2203225, 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35944441

RESUMO

Cooperative coupling of photocatalytic H2 O2 production with organic synthesis has an expansive perspective in converting solar energy into storable chemical energy. However, traditional powder photocatalysts suffer from severe agglomeration, limited light absorption, poor gas reactant accessibility and reusable difficulty, which greatly hinders their large-scale application. Herein, floatable composite photocatalysts are synthesized by immobilizing hydrophobic TiO2 and Bi2 O3 on light-weight polystyrene (PS) spheres via hydrothermal and photodeposition methods. The floatable photocatalysts are not only solar transparent, but also upgrade the contact between reactants and photocatalysts. Thus, the floatable step-scheme (S-scheme) TiO2 /Bi2 O3 photocatalyst exhibits a drastically enhanced H2 O2 yield of 1.15 mM h-1 and decent furfuryl alcohol conversion to furoic acid synchronously. Furthermore, the S-scheme mechanism and dynamics are systematically investigated by in-situ irradiated X-ray photoelectron spectroscopy and femtosecond transient absorption spectrum analyses. In-situ Fourier transform infrared spectroscopy and density functional theory (DFT) calculations reveal the mechanism of furoic acid evolution. The ingenious design of floatable photocatalysts not only furnishes insight into maximizing photocatalytic reaction kinetics but also provides a new route for high-efficient heterogeneous catalysis. This article is protected by copyright. All rights reserved.

7.
Oncogene ; 2022 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-35798876

RESUMO

Circular RNAs (circRNAs) play critical roles in clear cell renal cell carcinoma (ccRCC). However, their involvement in sunitinib resistance remains largely unknown. Herein, we identified a novel circRNA, named circME1, which contributes to sunitinib resistance development in ccRCC. CircME1 also promoted proliferation, migration, and invasion of ccRCC cells. Further mechanism analysis showed that circME1 interacted with U1 snRNP at the promoter of its parental gene ME1, thereby upregulating the expression of ME1, enhancing aerobic glycolysis of ccRCC, and promoting its malignant phenotype. Furthermore, ME1 specific inhibitor could effectively repress the oncogenic functions of circME1. Taken together, our study demonstrates that the circME1/ME1 pathway is involved in ccRCC progression and sunitinib resistance development, which may be exploited for anticancer therapy.

8.
Biomed Opt Express ; 13(6): 3295-3310, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35781965

RESUMO

To expand the clinical applications and improve the ease of use of ultrahigh-resolution optical coherence tomography (UHR-OCT), we developed a portable boom-type ophthalmic UHR-OCT operating in supine position that can be used for pediatric subjects, bedridden patients and perioperative conditions. By integrating the OCT sample arm probe with real-time iris display and automatic focusing electric lens for easy alignment, coupling the probe on a self-locking multi-directional manipulator to reduce motion artifacts and operator fatigue, and installing the OCT module on a moveable cart for system mobility, our customized portable boom-type UHR-OCT enables non-contact, high-resolution and high-stability retinal examinations to be performed on subjects in supine position. The spectral-domain UHR-OCT operates at a wavelength of 845 nm with 130 nm FWHM (full width at half maximum) bandwidth, achieving an axial resolution of ≈2.3µm in tissue with an A-line acquisition rate up to 128 kHz. A high-definition two-dimensional (2D) raster protocol was used for high-quality cross-sectional imaging while a cube volume three-dimensional (3D) scan was used for three-dimensional imaging and en-face reconstruction, resolving major layer structures of the retina. The feasibility of the system was demonstrated by performing supine position 2D/3D retinal imaging on healthy human subjects, sedated infants, and non-sedated awake neonates.

9.
Front Public Health ; 10: 915716, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35784212

RESUMO

Objective: To evaluate epidemiological characteristics of the COVID-19 outbreak that resurged in Yangzhou and to simulate the impact of different control measures at different regional scales. Methods: We collected personal information from 570 laboratory-confirmed cases in Yangzhou from 28 July to 26 August 2021, and built a modified susceptible-exposed-infected-removed (SEIR) model and an agent-based model. Results: The SEIR model showed that for passengers from medium-high risk areas, pre-travel nucleic acid testing within 3 days could limit the total number of infected people in Yangzhou to 50; among elderly persons, a 60% increase in vaccination rates could reduce the estimated infections by 253. The agent-based model showed that when the population density of the chess and card room dropped by 40%, the number of infected people would decrease by 54 within 7 days. A ventilation increase in the chess and card room from 25 to 50% could reduce the total number of infections by 33 within 7 days; increasing the ventilation from 25 to 75% could reduce the total number of infections by 63 within 7 days. Conclusions: The SEIR model and agent-based model were used to simulate the impact of different control measures at different regional scales successfully. It is possible to provide references for epidemic prevention and control work.


Assuntos
COVID-19 , Epidemias , Idoso , COVID-19/epidemiologia , China/epidemiologia , Simulação por Computador , Surtos de Doenças/prevenção & controle , Humanos
10.
Comput Math Methods Med ; 2022: 6846079, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35799639

RESUMO

Background: The effect of total intravenous anesthesia (TIVA) and inhalation anesthesia (IA) on the prognosis of breast cancer patients has been controversial. The study is aimed at exploring the effects of different anesthesia methods on the postoperative prognosis of breast cancer patients. Methods: Literature retrieval was conducted in PubMed, EMBASE, MEDLINE, Embase, CENTRAL, and CNKI databases. The literature topic was to compare the effects of TIVA and IA on the prognosis of patients undergoing breast cancer resection. Two researchers extracted data from the literature independently. This study included randomized controlled trials that evaluated for risk of bias according to the "Risk assessment Tool for Bias in Randomized Trials" in the Cochrane Manual. The Newcastle-Ottawa Scale (NOS) was used to assess the risk of bias in observational studies. The chi-square test was used for the heterogeneity test. Publication bias was assessed using funnel plots and Egger's test. If heterogeneity existed between literature, subgroup analysis and sensitivity analysis were used to explore the source of heterogeneity. Sensitivity analysis was performed by excluding low-quality and different-effect models. Data were statistically analyzed using the Cochrane software RevMan 5.3. Hazard ratio (HR) and 95% confidence interval (CI) were used for statistical description. Results: Seven literatures were selected for meta-analysis. There were 9781 patients, 3736 (38.20%) receiving TIVA and 6045 (61.80%) receiving inhalation anesthesia. There was no significant difference in overall survival (OS) between TIVA and IA breast cancer patients (HR = 1.05, 95% CI (0.91, 1.22), Z = 0.70, P = 0.49). There was no difference in the literature (χ 2 = 6.82, P = 0.34, I 2 = 12%), and there was no obvious publication bias. There was no significant difference in recurrence-free survival (RFS) between TIVA and IA patients (HR = 0.95, 95% CI (0.79, 1.13), Z = 0.61, P = 0.54). There was no heterogeneity in the literature (χ 2 = 5.23, P = 0.52, I 2 = 0%), and there was no significant publication bias. Conclusion: There is no significant difference in OS and RFS between TIVA and IA patients during breast cancer resection. The prognostic effects of TIVA and IA were similar.


Assuntos
Neoplasias da Mama , Anestesia por Inalação/métodos , Neoplasias da Mama/cirurgia , Feminino , Humanos , Prognóstico , Ensaios Clínicos Controlados Aleatórios como Assunto
11.
Sensors (Basel) ; 22(13)2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35808190

RESUMO

Low-dimensional transition-metal dichalcogenides (TMDs) have recently emerged as promising materials for electronics and optoelectronics. In particular, photodetectors based on mono- and multilayered molybdenum disulfide (MoS2) have received much attention owing to their outstanding properties, such as high sensitivity and responsivity. In this study, photodetectors based on dispersed MoS2 nanoflakes (NFs) are demonstrated. MoS2 NFs interact with Ag nanoparticles (NPs) via low-temperature annealing, which plays a crucial role in determining device characteristics such as good sensitivity and short response time. The fabricated devices exhibited a rapid response and recovery, good photo-responsivity, and a high on-to-off photocurrent ratio under visible light illumination with an intensity lower than 0.5 mW/cm2.

12.
Epileptic Disord ; 24(5): 1-9, 2022 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-35816100

RESUMO

Objective: Despite the potential role of valproic acid (VPA) in weight gain, the effects of VPA therapy on lipid profiles remain unclear. This study aimed to review the influence of VPA therapy on serum lipid profiles in children with epilepsy. Methods: This meta-analysis was conducted on data from PubMed, Web of Science, Cochrane Library, and Embase databases. Case-controlled studies, which assessed the effects of VPA therapy on lipid profiles, were included. All outcomes were recorded as continuous variables, and the effect size was measured. Results: VPA therapy was associated with a significant reduction in total cholesterol (mean difference [MD]=-6.34, 95% confidence interval [CI]: -12.30, -0.37, p=0.04) and low-density lipoprotein cholesterol levels (MD = -7.75, 95% CI: -13.48, -2.0, p=0.008). No significant effects were observed regarding the levels of high-density lipoprotein cholesterol and triglycerides. Significance: In conclusion, this meta-analysis indicates that VPA therapy causes a decrease in the levels of total cholesterol and low-density lipoprotein cholesterol.


Assuntos
Epilepsia , Ácido Valproico , Criança , HDL-Colesterol , LDL-Colesterol , Epilepsia/tratamento farmacológico , Humanos , Lipídeos , Ácido Valproico/farmacologia , Ácido Valproico/uso terapêutico
13.
Mol Neurobiol ; 2022 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-35829831

RESUMO

Neurodegenerative diseases (NDs), including chronic disease such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and multiple sclerosis, and acute diseases like traumatic brain injury and ischemic stroke are characterized by progressive degeneration, brain tissue damage and loss of neurons, accompanied by behavioral and cognitive dysfunctions. So far, there are no complete cures for NDs; thus, early and timely diagnoses are essential and beneficial to patients' treatment. Magnetic resonance imaging (MRI) has become one of the advanced medical imaging techniques widely used in the clinical examination of NDs due to its non-invasive diagnostic value. In this review, research published in English in current decade from PubMed electronic database on the use of MRI to detect specific biomarkers of NDs was collected, summarized, and discussed, which provides valuable suggestions for the early diagnosis, prevention, and treatment of NDs in the clinic.

14.
Artigo em Inglês | MEDLINE | ID: mdl-35895648

RESUMO

Inspired by sparse learning, the Markowitz mean-variance model with a sparse regularization term is popularly used in sparse portfolio optimization. However, in penalty-based portfolio optimization algorithms, the cardinality level of the resultant portfolio relies on the choice of the regularization parameter. This brief formulates the mean-variance model as a cardinality ( l0 -norm) constrained nonconvex optimization problem, in which we can explicitly specify the number of assets in the portfolio. We then use the alternating direction method of multipliers (ADMMs) concept to develop an algorithm to solve the constrained nonconvex problem. Unlike some existing algorithms, the proposed algorithm can explicitly control the portfolio cardinality. In addition, the dynamic behavior of the proposed algorithm is derived. Numerical results on four real-world datasets demonstrate the superiority of our approach over several state-of-the-art algorithms.

15.
Artigo em Inglês | MEDLINE | ID: mdl-35886570

RESUMO

Ionic liquids (ILs) are known as "green solvents" and widely used in industrial applications. However, little research has been conducted on cyanobacteria. This study was conducted to investigate the toxicity of ionic liquids ([Hmim]Cl) on Microcystis aeruginosa PCC7806. The EC50 (72 h) of [Hmim]Cl on the growth of Microcystis aeruginosa PCC 7806 was 10.624 ± 0.221 mg L-1. The possible mechanism of toxicity of [Hmim]Cl against M. aeruginosa PCC 7806 was evaluated by measuring cell growth, photosynthetic pigment contents, chlorophyll fluorescence transients, cell ultrastructure, and transcription of the microcystin-producing gene (mcyB). The concentrations of chlorophyll a and carotenoids were significantly reduced in treated M. aeruginosa cultures. The results of chlorophyll fluorescence transients showed that [Hmim]Cl could destruct the electron-accepting side of the photosystem II of M. aeruginosa PCC 7806. Transmission electron microscopy demonstrated cell damage including changes in the structure of the cell wall and cell membrane, thylakoid destruction, and nucleoid disassembly. The transcription of the mcyB gene was also inhibited under [Hmim]Cl stress. In summary, this study provides new insights into the toxicity of [Hmim]Cl on cyanobactreia.


Assuntos
Líquidos Iônicos , Microcystis , Clorofila/metabolismo , Clorofila A , Líquidos Iônicos/toxicidade , Microcistinas/metabolismo , Fotossíntese , Complexo de Proteína do Fotossistema II/metabolismo
16.
ACS Appl Mater Interfaces ; 14(28): 31645-31654, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35790212

RESUMO

Atopic dermatitis (AD) is a chronic inflammatory skin disease that seriously affects the life quality of patients. Topical administration of glucocorticoids is considered to be the most effective anti-inflammatory treatment. However, due to the barrier function of skin, only less than 20% of topical drug molecules could diffuse into the skin. Therefore, it is of great importance to develop an effective strategy to improve AD therapy. In this study, we reported a two-electrode microneedle patch (t-EMNP) composed of a polylactic acid-platinum (PLA-Pt) MN array and polylactic acid-platinum-polypyrrole (PLA-Pt-PPy) MN array for improving the transdermal drug delivery efficacy. The drug loading capability of MNs could be altered by employing different polymerization times and drug concentrations. The drug release rate of MNs could be changed by applying different voltages. We further developed a controlled transdermal drug delivery system (c-TDDS) based on this two-electrode microneedle patch (t-EMNP), exhibiting the remarkable performance of the electricity-triggered drug release profile. The drugs could be released with electrical stimulation, while there was almost no drug release without electrical stimulation. For AD treatment in vivo, this MN patch with electricity-triggered drug release performance could effectively deliver more drugs into the skin compared with other controls such as dexamethasone cream, which efficiently alleviate AD. In sum, this work not only developed a smart patch for improving AD treatment but also provided a promising approach of transdermal drug delivery on demand.


Assuntos
Dermatite Atópica , Polímeros , Dermatite Atópica/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Eletricidade , Humanos , Microinjeções , Agulhas , Preparações Farmacêuticas , Platina , Poliésteres , Pirróis , Pele
17.
Sci Transl Med ; 14(654): eabn1413, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35857825

RESUMO

To combat the HIV epidemic and emerging threats such as SARS-CoV-2, immunization strategies are needed that elicit protection at mucosal portals of pathogen entry. Immunization directly through airway surfaces is effective in driving mucosal immunity, but poor vaccine uptake across the mucus and epithelial lining is a limitation. The major blood protein albumin is constitutively transcytosed bidirectionally across the airway epithelium through interactions with neonatal Fc receptors (FcRn). Exploiting this biology, here, we demonstrate a strategy of "albumin hitchhiking" to promote mucosal immunity using an intranasal vaccine consisting of protein immunogens modified with an amphiphilic albumin-binding polymer-lipid tail, forming amph-proteins. Amph-proteins persisted in the nasal mucosa of mice and nonhuman primates and exhibited increased uptake into the tissue in an FcRn-dependent manner, leading to enhanced germinal center responses in nasal-associated lymphoid tissue. Intranasal immunization with amph-conjugated HIV Env gp120 or SARS-CoV-2 receptor binding domain (RBD) proteins elicited 100- to 1000-fold higher antigen-specific IgG and IgA titers in the serum, upper and lower respiratory mucosa, and distal genitourinary mucosae of mice compared to unmodified protein. Amph-RBD immunization induced high titers of SARS-CoV-2-neutralizing antibodies in serum, nasal washes, and bronchoalveolar lavage. Furthermore, intranasal amph-protein immunization in rhesus macaques elicited 10-fold higher antigen-specific IgG and IgA responses in the serum and nasal mucosa compared to unmodified protein, supporting the translational potential of this approach. These results suggest that using amph-protein vaccines to deliver antigen across mucosal epithelia is a promising strategy to promote mucosal immunity against HIV, SARS-CoV-2, and other infectious diseases.


Assuntos
COVID-19 , Infecções por HIV , Administração Intranasal , Albuminas , Animais , Anticorpos Antivirais , COVID-19/prevenção & controle , Infecções por HIV/prevenção & controle , Imunidade nas Mucosas , Imunoglobulina A , Imunoglobulina G , Lipídeos , Macaca mulatta , Camundongos , Camundongos Endogâmicos BALB C , SARS-CoV-2 , Vacinação
18.
Front Microbiol ; 13: 923435, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35859745

RESUMO

Currently, mushroom poisoning still poses a huge problem to humans' health and life globally. Poisoning incidents caused by Inosperma spp. were reported continuously in tropical China in recent years. In this study, a new poisonous Inosperma species, discovered from a poisoning incident, was described in tropical China based on morphological, molecular, and toxin detection evidence; detailed descriptions, photographs, and comparisons to closely related species were provided. For qualitative analysis, through targeted screening using ultra-high liquid chromatography triple quadrupole mass spectrometry (UPLC-MS/MS), the new species contains muscarine and no other toxins (two isoxazole derivatives, two tryptamine alkaloids, three amatoxins, and three phallotoxins). For quantitative analysis, muscarine contents in the pileus and the stipe were 2.08 ± 0.05 and 6.53 ± 1.88 g/kg, respectively.

19.
Front Pharmacol ; 13: 816683, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35873553

RESUMO

Background: Alectinib, a highly selective inhibitor of ALK, is currently used in the first-line setting of untreated advanced ALK-positive NSCLC and in the second-line setting of crizotinib-resistant ALK-positive NSCLC. Despite promising efficacy and tolerability in the treatment of advanced ALK-positive NSCLC, the activity of alectinib as neoadjuvant therapy in resectable ALK-positive NSCLC remains to be investigated. Case presentation: Herein, we report a case of a 58-year-old female patient presented to our hospital with hemoptysis for 1 month. Contrast-enhanced computerized tomography (CT) of the chest showed an approximately 4.2 × 3.4 cm mass in the right hilum with localized obstructive pneumonia in the right lower lobe and multiple enlarged lymph nodes in the right hilum and mediastinum. Serum oncological markers results showed elevated levels of CA19-9, CEA, CA125, and CA242. Bronchoscopic biopsy of the mass showed poorly differentiated pulmonary adenocarcinoma and immunohistochemical testing results confirmed ALK positivity. Neoadjuvant alectinib was given at a dosage of 600 mg twice per day for two cycles (56 days), achieving a partial response of the disease with 90% shrinkage of the mass at the subsequent whole-body positron emission tomography. Repeat serum oncological markers results showed that only CA125 was elevated, but lower than before therapy. A bilobectomy of the right middle and lower lobes and systemic lymphadectomy under video-assisted thoracoscopic approach was successfully performed 7 days after the last dose of alectinib. Postoperative pathology showed pathological complete response (pCR). The patient experienced an uneventful postoperative course and continued to receive alectinib and did not report any specific discomfort at her 8-month follow-up. Thoracoabdominal CT at 8 months postoperatively showed no recurrence and repeated examination of serum oncological markers were negative. Conclusion: We report a case of resectable ALK-positive NSCLC treated with neoadjuvant aletinib achieving pCR. Our case highlights the feasibility of alectinib as neoadjuvant therapy for the treatment of resectable ALK-positive NSCLC. Undoubtedly, the safety and efficacy of this novel treatment modality needs to be explored in future large clinical trials.

20.
Heliyon ; 8(7): e09821, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35855985

RESUMO

Objective: To analyze surveys measuring the prevalence of burnout among Chinese doctors and reveal the overall prevalence, characteristics, timeline, and factors related to burnout. Methods: A comprehensive search was conducted on China National Knowledge Infrastructure, WANFANG, PubMed, EMBASE, PsycINFO and Cochrane Library databases from their inception to 28 February 2021. Random-effects meta-analyses, meta-regression and planned subgroup analyses were performed, and the standardized mean difference was adopted for comparisons between subgroups. Egger's and Begg's tests were performed to evaluate publication bias. Heterogeneity across the studies was tested using the I2 statistic. The study protocol was registered on PROSPERO (CRD42018104249). Results: In total, 3,210 records were reviewed; 64 studies including 48,638 Chinese doctors were eligible for meta-analysis. The prevalence of burnout increased continuously from 2008 to 2017 and decreased significantly from 2018 to 2020, a little increase from 2020 to 2021. The overall prevalence of burnout was 75.48% (95% CI, 69.20 to 81.26; I2 = 99.23%, P < 0.001), and high burnout was 9.37% (95% CI, 4.91 to 15.05, I2 = 98.88%, P < 0.001). The prevalence of emotional exhaustion was 48.64% (95% CI, 38.73 to 58.59; I2 = 99.53%, P < 0.001), depersonalization was 54.67% (95% CI, 46.95 to 62.27; I2 = 99.20%, P < 0.001), and reduced personal accomplishment was 66.53% (95% CI, 58.13 to 74.44; I2 = 99.37%, P < 0.001). Gender, marriage, professional title and specialty all influenced burnout. Conclusions: The results showed that the total prevalence of doctor burnout in China is very high. The prevalence of burnout varies by location. Gender, marital status and professional title all affect burnout scores.

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