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1.
FASEB J ; 35(9): e21667, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34405442

RESUMO

Long noncoding RNAs (lncRNAs) are central regulators of the inflammatory response and play an important role in inflammatory diseases. PINT has been reported to be involved in embryonic development and tumorigenesis. However, the potential functions of PINT in the innate immune system are largely unknown. Here, we revealed the transcriptional regulation of inflammatory genes by PINT, whose expression is primarily dependent on the NF-κB signaling pathway in human and mouse macrophage and intestinal epithelial cell lines. Functionally, PINT selectively regulates the expression of TNF-α in basal and LPS-stimulated cells. Mechanistically, PINT acts as a modular scaffold of p65 and EZH2 to coordinate their localization and specify their binding to the target genes. Further, a high expression level of PINT was detected in intestinal mucosal tissues from patients with ulcerative colitis (UC). Together, these findings demonstrate that PINT acts as an activator of inflammatory responses, highlighting the importance of this lncRNA as a potential therapeutic target in infectious diseases and inflammatory diseases.


Assuntos
Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Regulação da Expressão Gênica , RNA Longo não Codificante/genética , Fator de Transcrição RelA/metabolismo , Transcrição Genética , Fator de Necrose Tumoral alfa/genética , Animais , Linhagem Celular , Colite Ulcerativa/genética , Colite Ulcerativa/patologia , Citocinas/genética , Humanos , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/patologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas/genética , Ligação Proteica , Transcrição Genética/genética
2.
J Healthc Eng ; 2021: 8719147, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34354809

RESUMO

A novel method for the detection of the hepatitis B surface antigen (HBsAg) at low concentrations, using the ultrahigh-order guided mode acting as the probe excited by a symmetrical metal-cladding waveguide, is proposed. The method using the fact of the minimum value of the absorption peaks is proportional to the concentration of the sample to be detected to realize the detection of the hepatitis B virus at extremely low concentrations. It is realized that the low concentration of the HBsAg measurement relied on the principle of the minimum value of the absorption peak and the concentration having a good linear relationship. The measurement results indicate that this new method can precisely detect HBsAg at the concentrations in the lower region of the clinical gray area (i.e., below 20 ng/mL), the lower region of the current clinical gray area of HBsAg (below 20 ng/ml) can be measured, and the resolution can be reached (2 ng/mL).

3.
Cardiovasc Ther ; 2021: 5534946, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34457036

RESUMO

This meta-analysis was conducted to analyze the effect of NQO1 polymorphism on the warfarin maintenance dosage. Using strict inclusion and exclusion criteria, we searched PubMed, EMBASE, and the Cochrane Library for eligible studies published prior to July 7, 2021. The required data were extracted, and experts were consulted when necessary. Review Manager Version 5.4 software was used to analyze the relationship between NQO1 polymorphisms and the warfarin maintenance dosage. Four articles involving 757 patients were included in the meta-analysis. Patients who were NQO1 rs10517 G carriers (AG carriers or GG carriers) required a 48% higher warfarin maintenance dose than those who were AA carriers. Patients with NQO1 rs1800566 CT carriers required a 13% higher warfarin dose than those who were CC carriers, with no associations observed with the other comparisons of the NQO1 rs1800566 genotypes. However, the results obtained by comparing the NQO1 rs1800566 genotypes require confirmation, as significant changes in the results were found in sensitivity analyses. Our meta-analysis suggests that the NQO1 rs10517and NQO1 rs1800566 variant statuses affect the required warfarin maintenance dose.

4.
Anal Chem ; 93(31): 10966-10973, 2021 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-34327982

RESUMO

Compared with free miRNAs in blood, miRNAs in exosomes have higher abundance and stability. Therefore, miRNAs in exosomes can be regarded as an ideal tumor marker for early cancer diagnosis. Here, a peptide nucleic acid (PNA)-functionalized nanochannel biosensor for the ultrasensitive and specific detection of tumor exosomal miRNAs is proposed. After PNA was covalently bound to the inner surface of the nanochannels, the detection of tumor exosomal miRNAs was achieved by the charge changes on the surface of nanochannels before and after hybridization (PNA-miRNA). Due to the neutral characteristics of PNA, the efficiency of PNA-miRNA hybridization was improved by significantly reducing the background signal. This biosensor could not only specifically distinguish target miRNA-10b from single-base mismatched miRNA but also achieve a detection limit as low as 75 aM. Moreover, the biosensor was further used to detect exosomal miRNA-10b derived from pancreatic cancer cells and normal pancreatic cells. The results indicate that this biosensor could effectively distinguish pancreatic cancer tumor-derived exosomes from the normal control group, and the detection results show good consistency with those of the quantitative reverse-transcription polymerase chain reaction method. In addition, the biosensor was used to detect exosomal miRNA-10b in clinical plasma samples, and it was found that the content of exosomal miRNA-10b in cancer patients was generally higher than that of healthy individuals, proving that the method is expected to be applied for the early diagnosis of cancer.


Assuntos
Técnicas Biossensoriais , Exossomos , MicroRNAs , Neoplasias , Ácidos Nucleicos Peptídicos , Exossomos/genética , Humanos , MicroRNAs/genética
5.
J Healthc Eng ; 2021: 6265520, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34136110

RESUMO

Wearable devices are gradually entering the medical health field. Medical Internet of Things (IoT) has been widely used in all walks of medical health. With the complexity of medical health application scenarios, the medical IoT communication networks face complex environments. The secure communication issue is very important for medical IoT communication networks. This paper investigates the secrecy performance of medical IoT communication networks. To improve the secrecy performance, we adopt a cooperative communication strategy. We also use the average secrecy capacity (ASC) as a metric, and the expressions are first derived. Then, a secrecy performance intelligent prediction algorithm is proposed. The extensive simulations are used to verify the proposed method. Compared with other methods, the proposed algorithm realizes a better prediction precision.

6.
Biochem Biophys Res Commun ; 549: 1-7, 2021 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-33647537

RESUMO

PURPOSE: Ixazomib is a selective, effective, and reversible inhibitor of 20S proteasome and is approved for the treatment of multiple myeloma. Ubiquitin-conjugating enzyme E2 (UBE2K) is involved in the synthesis of K48-linked ubiquitin chains and is the target of certain drugs used for the treatment of tumors. The purpose of this study was to investigate the relationship between ixazomib and UBE2K in myeloma cells. METHODS: We used CCK-8 and Annexin V-FITC/propidium iodide kit to detect the effects of ixazomib on survival and apoptosis of RPMI-8226 and U-266 myeloma cell lines. Quantitative polymerase chain reaction and western blot were used to detect the change in gene and protein expression levels of myeloma cells treated with ixazomib. Furthermore, the regulatory effects of ixazomib on UBE2K and its downstream targets were investigated following the overexpression of UBE2K. RESULTS: In myeloma cells, ixazomib decreased cell survival and increased apoptosis in a dose-dependent manner. Ixazomib significantly increased the expression of HIST1H2BD, MNAT1, NEK3, and TARS2, while decreasing the expression of HSPA1B and UBE2K. In addition, ixazomib inhibited the proliferation of myeloma cells, blocked cell cycle, induced cell apoptosis, and increased the production of reactive oxygen species by inhibiting UBE2K expression. Lastly, ixazomib regulates mitosis- and apoptosis-related genes by lowering UBE2K expression. CONCLUSION: In summary, ixazomib leads to impaired proliferation of myeloma cells by targeting UBE2K.


Assuntos
Compostos de Boro/uso terapêutico , Glicina/análogos & derivados , Mieloma Múltiplo/tratamento farmacológico , Mieloma Múltiplo/patologia , Enzimas de Conjugação de Ubiquitina/metabolismo , Apoptose/efeitos dos fármacos , Biomarcadores Tumorais/metabolismo , Compostos de Boro/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Glicina/farmacologia , Glicina/uso terapêutico , Humanos , Mitose/efeitos dos fármacos , Mieloma Múltiplo/genética
7.
Exp Cell Res ; 360(2): 273-280, 2017 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-28912086

RESUMO

Senescent cells have lost their capacity for proliferation and manifest as irreversibly in cell cycle arrest. Many membrane receptors, including G protein-coupled receptors (GPCRs), initiate a variety of intracellular signaling cascades modulating cell division and potentially play roles in triggering cellular senescence response. GPCR kinases (GRKs) belong to a family of serine/threonine kinases. Although their role in homologous desensitization of activated GPCRs is well established, the involvement of the kinases in cell proliferation is still largely unknown. In this study, we isolated GRK4-GFP expressing HEK293 cells by fluorescence-activated cell sorting (FACS) and found that the ectopic expression of GRK4 halted cell proliferation. Cells expressing GRK4 (GRK4(+)) demonstrated cell cycle G1/G0 phase arrest, accompanied with significant increase of senescence-associated-ß-galactosidase (SA-ß-Gal) activity. Expression profiling analysis of 78 senescence-related genes by qRT-PCR showed a total of 17 genes significantly changed in GRK4(+) cells (≥ 2 fold, p < 0.05). Among these, 9 genes - AKT1, p16INK4, p27KIP1, p19INK4, IGFBP3, MAPK14, PLAU, THBS1, TP73 - were up-regulated, while 8 genes, Cyclin A2, Cyclin D1, CDK2, CDK6, ETS1, NBN, RB1, SIRT1, were down-regulated. The increase in cyclin-dependent kinase inhibitors (p16, p27) and p38 MAPK proteins (MAPK14) was validated by immunoblotting. Neither p53 nor p21Waf1/Cip1 protein was detectable, suggesting no p53 activation in the HEK293 cells. These results unveil a novel function of GRK4 on triggering a p53-independent cellular senescence, which involves an intricate signaling network.


Assuntos
Senescência Celular/genética , Quinase 4 de Receptor Acoplado a Proteína G/fisiologia , Perfilação da Expressão Gênica , Divisão Celular/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Citometria de Fluxo , Regulação da Expressão Gênica/genética , Células HEK293 , Humanos , Células MCF-7 , Transcriptoma , Proteína Supressora de Tumor p53/fisiologia
8.
Res Vet Sci ; 115: 96-101, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28199901

RESUMO

Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is responsible for peritonitis, septicemia, meningitis, arthritis and several other serious diseases in various species. Recent studies have demonstrated that CD44 is implicated in the process of host defense against pathogenic microorganisms. In the present study, the role of CD44 in the host response to S. zooepidemicus infection was investigated in a mouse model. Upon intraperitoneal infection with S. zooepidemicus, the expression of CD44 on the peritoneal exudate cells from wild-type (WT) mice was increased. CD44 deficiency accelerated mortality, which was accompanied by increased peritoneal bacterial growth and dissemination to distant body sites. CD44 knock-out (KO) mice showed enhanced early inflammatory cell recruitment into the peritoneal fluid on S. zooepidemicus infection. In line with this, the expression of proinflammatory cytokines, chemokines in peritoneal exudate cells and peritoneal macrophages of CD44 KO mice were increased compared with those of WT mice. In addition, CD44 deficiency was associated with reduced expression of A20, a negative regulator in TLR signaling. Overall, the present study suggests that CD44 plays a protective role in antibacterial defense against S. zooepidemicus in mice.


Assuntos
Receptores de Hialuronatos/genética , Inflamação/patologia , Infecções Estreptocócicas/microbiologia , Streptococcus equi/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Macrófagos Peritoneais , Camundongos , Camundongos Knockout , Infecções Estreptocócicas/patologia
9.
Vet Microbiol ; 198: 121-126, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28062002

RESUMO

Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is an important pathogen associated with a wide range of diseases in many mammalian species. CD44 is a transmembrane adhesion molecule involved in innate and adaptive immune responses. The aim of this study was to determine the protective role of CD44 during S. zooepidemicus infection. CD44-deficient mice exhibited reduced macrophage accumulation in the bronchoalveolar space and enhanced bacterial outgrowth and dissemination, which resulted in reduced mouse survival. An in vitro analysis revealed that CD44 can directly bind to S. zooepidemicus. Additionally, S. zooepidemicus interacted with macrophage-associated CD44, as reflected by the reduced uptake of S. zooepidemicus by CD44-deficient macrophages. These data suggest that CD44 contributes to effective antibacterial defense during S. zooepidemicus infection, thereby limiting the accompanying injury and death.


Assuntos
Doenças dos Cavalos/genética , Doenças dos Cavalos/imunologia , Receptores de Hialuronatos/metabolismo , Imunidade/genética , Macrófagos/imunologia , Fagocitose , Infecções Estreptocócicas/veterinária , Animais , Cavalos , Receptores de Hialuronatos/genética , Macrófagos/microbiologia , Camundongos , Fagocitose/genética , Fagocitose/imunologia , Infecções Estreptocócicas/genética , Infecções Estreptocócicas/imunologia , Streptococcus equi/fisiologia , Análise de Sobrevida
10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(2): 545-9, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-27209766

RESUMO

Temperature is one of the most important parameters in studying of laser-induced plasma characteristics. To reduce the impact of continuous background on the calculation of temperatures using Boltzmann plots, the wavelet transform was used to decompose the spectrums, and the low-frequency signals represented the spectral baseline were deducted by using soft-threshold method. Selecting the appropriate wavelet decomposition level L and threshold coefficient a can increase the linear regression coefficient R2 of Boltzmann plots, and the calculation accuracy of plasma temperature was improved. The LIBS spectra of low alloy steel sample region from 417 to 445 nm were decomposed by using db4 wavelet, and then baseline subtraction and signal reconstruction were carried out, respectively. Twelve Fe atomic lines were chosen to establish Boltzmann plots, and the temperatures were calculated from the slope of the fitted lines in the plots. The value L and a were optimized according R², the results showed that the 8-layer db4 wavelet decomposition can gain the high R², while the value of a associated with the delay time td, e. g. , the optimum a corresponding to maximum values of R² is 0.3 when td ≤ 4.0 µs, and then decrease with the increasing of td, and reduced to 0 when td ≥ 6. 0 µs. The interference due to baseline on the spectral characteristic lines gradually reduced with the increasing of td, and therefore a decreased with td increase. After the baseline was deducted, the temperature calculated by Boltzmann plot decrease of about 2 000 to 3 000 K. The temperature gradually decreased with the increasing of the td, and the temperature fluctuation is reduced after baseline subtraction, these results are consistent with the physical process of plasma expansion.

11.
Res Vet Sci ; 97(3): 498-504, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25294252

RESUMO

Porcine circovirus type 2 (PCV2) is the primary etiological agent of postweaning multisystemic wasting syndrome (PMWS). CD44 is a widely expressed class I transmembrane glycoprotein implicated in immunological and inflammatory responses. In previous studies, the role of CD44 in host defense against microorganism infection remains controversial. The role of CD44 in host defense against PCV2 infection has never been studied before. In this study, we investigated the role of CD44 in the development of pneumonia induced by PCV2 in mice model. Upon infection, CD44 mRNA level in lung tissue was upregulated, and we confirmed a detrimental role of CD44 in host defense against PCV2 infection. The results demonstrated that CD44 deficiency could result in decreased proinflammatory cytokine production in lung induced by PCV2 in mice, suggesting a previously unrecognized role for CD44 in the development of pneumonia response to PCV2 infection.


Assuntos
Infecções por Circoviridae/genética , Circovirus/fisiologia , Citocinas/genética , Receptores de Hialuronatos/genética , Animais , Infecções por Circoviridae/imunologia , Infecções por Circoviridae/virologia , Citocinas/metabolismo , Feminino , Receptores de Hialuronatos/metabolismo , Pulmão/imunologia , Pulmão/fisiopatologia , Pulmão/virologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Organismos Livres de Patógenos Específicos
12.
J Microbiol Biotechnol ; 23(4): 579-85, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23568215

RESUMO

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.


Assuntos
Adesinas Bacterianas/imunologia , Vacinas Bacterianas/imunologia , Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/imunologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus equi/enzimologia , Streptococcus equi/imunologia , Adesinas Bacterianas/genética , Adesinas Bacterianas/isolamento & purificação , Adesinas Bacterianas/metabolismo , Animais , Anticorpos Antibacterianos/sangue , Aderência Bacteriana , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/genética , Vacinas Bacterianas/isolamento & purificação , Linhagem Celular , Modelos Animais de Doenças , Perfilação da Expressão Gênica , Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/genética , Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/isolamento & purificação , Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/metabolismo , Hepatócitos/microbiologia , Humanos , Camundongos , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Infecções Estreptocócicas/imunologia , Análise de Sobrevida , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/isolamento & purificação
13.
Vaccine ; 31(10): 1400-5, 2013 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-23306366

RESUMO

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including horses, pigs and humans. The absence of suitable vaccine confounds the control of SEZ infection. Cell surface protein (CSP) has been identified as an immunogenic protein in the previous study but its protective efficacy is not clear. In the present study, the purified recombinant CSP could elicit a significant humoral antibody response and could confer significant protection against challenge with lethal dose of SEZ in mice model. CSP could adhere to the HEp-2 cells confirmed by flow cytometry and inhibit adherence of SEZ to HEp-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that CSP was induced in vivo following infection of mice with SEZ. Our findings suggest that CSP may play a potential role in the pathogenesis of SEZ and could be a target for the development of a novel subunit vaccine against SEZ infection.


Assuntos
Antígenos de Bactérias/imunologia , Proteínas de Membrana/imunologia , Vacinas Estreptocócicas/imunologia , Streptococcus equi/imunologia , Animais , Antígenos de Bactérias/genética , Aderência Bacteriana/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Células Hep G2 , Hepatócitos/microbiologia , Humanos , Proteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos BALB C , Infecções Estreptocócicas/prevenção & controle , Vacinas Estreptocócicas/administração & dosagem , Vacinas Estreptocócicas/genética , Análise de Sobrevida , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia
14.
Opt Express ; 20(13): 13833-40, 2012 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-22714448

RESUMO

A new scheme is proposed to measure the electro-optical (EO) and converse-piezoelectric (CPE) coefficients of the PMN-PT ceramics simultaneously, in which the PMN-PT ceramics acts as the guiding layer of a symmetrical metal-cladding waveguide. As the applied electric field exerts on the waveguide, the effective refractive index (RI) (or synchronous angle) can be effectively tuned from a selected mode to another adjacent mode owing to the high sensitivity and the small spacing of the ultra-high order modes. Subsequently, a correlation between EO and CPE coefficients is established. With this correlation and the measurement of the effective RI change to the applied voltage, the quadratic EO and CPE coefficients of PMN-PT ceramics are obtained simultaneously. The obtained results are further checked by fitting the variations of effective RI to a quadratic function. Our measurement method can be extended to a wide range of other materials.


Assuntos
Cerâmica/química , Sistemas Microeletromecânicos/instrumentação , Dispositivos Ópticos , Refratometria/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , Luz , Espalhamento de Radiação
15.
Vet Microbiol ; 159(3-4): 519-25, 2012 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-22613253

RESUMO

Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is responsible for a wide variety of infections in many species. Attempts to control the infection caused by this agent are hampered by a lack of effective vaccines and useful diagnostic kits. Surface proteins of bacterial species are usually involved in interaction with host and hopefully act as biomarkers for serodiagnosis and subunit vaccine components. In this study, the surface proteins of SEZ C55138 strain were systematically identified by surface shaving with trypsin and a total of 20 surface associated proteins were found. Further analysis of five selected novel proteins (SzM, FBP, SAP, CSP and 5'-Nu) revealed that they all expressed in vivo and their recombinant derived proteins could be reactive with convalescent sera. These identified immunogenic surface proteins have potential as SEZ vaccine candidates and diagnostic markers.


Assuntos
Antígenos de Bactérias/imunologia , Streptococcus equi/química , Streptococcus equi/imunologia , Proteínas de Bactérias/imunologia , Immunoblotting , Proteínas de Membrana/imunologia , Proteínas Recombinantes/imunologia , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/microbiologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus equi/classificação , Vacinas de Subunidades/imunologia
16.
Opt Lett ; 36(19): 3837-9, 2011 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-21964114

RESUMO

We present an ultrafine tunable single-frequency Brillouin fiber laser in which the fiber Brillouin gain also acts as a high-precision tunable filter. Controlling the strain on the fiber, the Stokes frequency shift may be precisely changed, and thus the lasing frequency can be tuned. Assisted by the frequency pulling effect, this method provides continuous ultrafine tuning ability for ~30% of the free spectral range without mode hopping, and can cover 271 MHz. The experiments showed that the laser achieves tuning step of 60 kHz.

17.
Dev Comp Immunol ; 34(12): 1237-41, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20692286

RESUMO

Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen, blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia coli exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection.


Assuntos
Cistatina B/imunologia , Edwardsiella tarda/imunologia , Infecções por Enterobacteriaceae/veterinária , Doenças dos Peixes/imunologia , Linguados , Macrófagos/imunologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Cistatina B/química , Cistatina B/genética , Cistatina B/farmacologia , Inibidores de Cisteína Proteinase/química , Inibidores de Cisteína Proteinase/genética , Inibidores de Cisteína Proteinase/farmacologia , Infecções por Enterobacteriaceae/imunologia , Linguados/genética , Expressão Gênica , Imunidade Inata , Rim/imunologia , Fases de Leitura Aberta , Filogenia , Reação em Cadeia da Polimerase , Proteínas Recombinantes/farmacologia , Alinhamento de Sequência
18.
Blood Coagul Fibrinolysis ; 20(2): 101-7, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19786937

RESUMO

The objective of the present study was to evaluate the effect of batroxobin agents on perioperative hemorrhage in thoracic surgery.We systematically searched Cochrane Library, Pubmed, EMBASE and the China Biological Medicine CD-ROM Databases up to August 2007. Reference lists of all included studies and of reviews related to the topic of the present systematic review were manually searched. Two reviewers independently identified the eligible studies, assessed their methodological quality and extracted data. Results of relevant outcomes were pooled together whenever possible, using RevMan software.Five randomized controlled trials involving 678 patients were included. Three trials were for pneumonectomy and two for cardiac surgery with cardiopulmonary bypass. The quality of the identified studies was generally poor. All the trials claimed randomized allocation, but allocation concealment was unclear. Blinding was not mentioned. Two trials found that batroxobin agents decreased intraoperative blood loss for pneumonectomy. Mean differences between the batroxobin agents group and the no-treatment group were -182.20 ml [95% confidence interval (CI), -207.48 to -156.92] and -131.32 ml (95% CI, -142.95 to -119.69), respectively, for these two trials. All included trials reported less drainage volume favoring the batroxobin agents group. Mean differences at different time points after operation ranged from -15 ml (95% CI, -31.77 to 1.77) to -150.60 ml (95% CI, -179.26 to -121.94). Although most of the differences between the batroxobin agents group and the no treatment group were statistically significant, clinical value was limited.There is not enough evidence supporting any benefit of batroxobin agents for hemorrhage during thoracic surgery.


Assuntos
Batroxobina/administração & dosagem , Perda Sanguínea Cirúrgica/prevenção & controle , Fibrinolíticos/administração & dosagem , Procedimentos Cirúrgicos Torácicos , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
19.
Opt Express ; 17(16): 13309-14, 2009 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-19654735

RESUMO

Electrical tuning of polarization beam splitting is demonstrated in the structure of symmetrical metal-cladding waveguide by introducing optically nonlinear material into both the coupling prism and the guiding layer. Due to the anisotropy of the coupling material, different excitation conditions for TE and TM modes are obtained, which results in polarization-dependent reflections and transmissions. And the splitting effect of the two orthogonally polarized beams can be manipulated through an electrical modulation of the guiding layer properties.


Assuntos
Metais , Modelos Teóricos , Dispositivos Ópticos , Refratometria/instrumentação , Transdutores , Simulação por Computador , Desenho Assistido por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Luz , Espalhamento de Radiação
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