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1.
Andrologia ; 51(9): e13351, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31264245

RESUMO

Until now, no reliable method is recognised in treating buried penis. This study explored a new method of penile length augmentation using acellular dermal matrix filler in infrapubic space combined with liposuction and penile suspensory release. Patients with "small-sized penis" received penile length augmentation procedure including suprapubic liposuction, penile suspensory ligament release and insertion of folded acellular dermal matrix between corpora cavernosa and pubis symphysis. Their penile length from tip to skin was measured pre-operatively and post-operatively. The post-operative complications and patients' satisfaction were also recorded. Fifteen adult male patients were included with the mean age of 33.2 ± 4.6 years old and BMI of 28.9 ± 5.3 kg/m2 . The average amount of liposuction was 430 ± 90.0 ml. The average penile length measured pre-operatively and post-operatively (on table and 3 months afterwards) was 3.0 ± 1.3 cm, 7.3 ± 2.1 cm and 5.4 ± 1.8 cm. The penile length has significantly increased by 4.3 ± 1.6 cm (on table) and 2.4 ± 0.8 cm (3 months post-operatively; p < 0.05). The post-operative complications included oedema of penis, ecchymosis of lower abdomen and poor wound healing. No patient was dissatisfied with the appearance and function. The new method using acellular dermal matrix combined with liposuction and penile suspensory ligament release is safe and effective. The method could be applied to selected patients with buried penis.


Assuntos
Derme Acelular , Preenchedores Dérmicos/uso terapêutico , Lipectomia/métodos , Doenças do Pênis/cirurgia , Pênis/cirurgia , Procedimentos Cirúrgicos Urológicos Masculinos/métodos , Adulto , Estudos de Viabilidade , Humanos , Ligamentos/cirurgia , Lipectomia/efeitos adversos , Masculino , Obesidade/complicações , Obesidade/terapia , Tamanho do Órgão , Satisfação do Paciente , Seleção de Pacientes , Doenças do Pênis/etiologia , Doenças do Pênis/patologia , Pênis/patologia , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Resultado do Tratamento , Procedimentos Cirúrgicos Urológicos Masculinos/efeitos adversos
2.
Zhonghua Nan Ke Xue ; 25(9): 802-810, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-32233207

RESUMO

Objective: To investigate the status quo of the diagnosis and treatment of male urethritis (MU) in urology and andrology. METHODS: According to The Guidelines for Clinical Diagnosis and Treatment of Sexually Transmitted Diseases (2017), we designed 27 questions on the prevalence, diagnosis, treatment, and prognosis of MU. Using these questions, we conducted a questionnaire investigation among urological, andrological and other relevant clinicians with different professional titles, followed by an analysis of the compliance of the doctors to the Guidelines. RESULTS: Totally, 116 valid questionnaires were collected from 86 urological, 28 andrological and 2 other relevant doctors, including 22 professors, 36 associate professors, 40 attending doctors and 16 resident doctors. MU was found mostly in those aged 20-40 years and more than half of the patients had a history of unclean sex, gonococcal urethritis significantly less prevalent than non-gonococcal, with Ureaplasma urealyticum as the most common pathogen of non-gonococcal urethritis. As for the compliance to the Guidelines in the diagnosis of MU, 22.73% of the professors, 16.67% of the associate professors, 15.00% of the attending doctors and 12.50% of the resident doctors examined the eyes, mouth and perianus (P > 0.05), 40.91% of the professors, 58.33% of the associate professors, 40.00% of the attending doctors and 37.50% of the resident doctors conducted HIV and syphilis screening (P > 0.05), and 86.36% of the professors, 77.78% of the associate professors, 70.00% of the attending doctors and 75.00% of the resident doctors performed genital mycoplasma screening (P > 0.05). Concerning the treatment of MU, 50.00% of the professors, 47.22% of the associate professors, 22.50% of the attending doctors and 43.75% of the resident doctors used anti-Chlamydia trachomatis drugs for gonococcal urethritis (P > 0.05), 0.00% of the professors, 11.11% of the associate professors, 5.00% of the attending doctors and 31.25% of the resident doctors prescribed 1g single-dose oral azithromycin for non-gonococcal urethritis (P < 0.05), 13.64% of the professors, 33.33% of the associate professors, 17.50% of the attending doctors and 6.25% of the resident doctors medicated persistent or recurrent non-gonococcal urethritis for >4 weeks (P > 0.05), 63.64% of the professors, 83.33% of the associate professors, 57.50% of the attending doctors and 62.50% of the resident doctors treated asymptomatic trachomatis and mycoplasma infections according to the proposed medication in the Guidelines (P > 0.05). As regards the results of treatment, the cure rate of gonococcal urethritis was 100.00% by professors, 97.22% by associate professors, 95.00% by attending doctors and 81.25% by resident doctors (P > 0.05), and that of non-gonococcal urethritis was 86.36% by professors, 61.11% by associate professors, 62.50% by attending doctors and 37.50% by resident doctors (P < 0.05). CONCLUSIONS: Urological and andrological clinicians do not strictly follow the Guidelines in the diagnosis and treatment of male urethritis. There are significant differences in the dosing of azithromycin and results of treatment of non-gonococcal urethritis among doctors with different professional titles, but not in the other aspects.


Assuntos
Infecções por Ureaplasma/tratamento farmacológico , Uretrite/tratamento farmacológico , Uretrite/terapia , Adulto , Andrologia , Azitromicina/administração & dosagem , Fidelidade a Diretrizes , Humanos , Masculino , Mycoplasma genitalium , Inquéritos e Questionários , Uretrite/microbiologia , Urologia , Adulto Jovem
3.
Zhonghua Nan Ke Xue ; 25(6): 522-528, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32223087

RESUMO

Objective: To investigate the effects of low-dose PDE5 inhibitors on metabolic parameters and erectile function in ED patients with subclinical metabolic syndrome (SCMS). METHODS: Totally, 132 ED patients, aged 21-61 (mean 34.5) years, were treated in the Andrology Clinic of the First Hospital of Wenzhou Medical University from April 2017 to May 2018. According to the diagnostic criteria, we divided the patients into groups A (simple ED, n = 40), B (ED with SCMS, n = 34) and C (ED with MS, n = 58) to receive 3 months of oral administration of tadalafil at 5 mg qd at bedtime, and followed them up for 3 months after drug withdrawal. During the treatment, we advised the patients to keep a healthy diet, change bad habits, participate in regular physical exercise, and maintain psychological balance. Before and right after medication and at 3 months after drug withdrawal, we recorded the changes in the IIEF-5 scores, abdominal circumference, blood pressure and levels of fasting blood sugar (FBS), triglyceride (TG) and high-density lipoprotein (HDL) of the patients. RESULTS: The IIEF-5 scores showed statistically significant differences at different time points between groups A and C (P < 0.01), remarkably higher right after treatment than before treatment and at 3 months after drug withdrawal in group B (19.71 ± 2.40 vs 10.21 ± 3.92 and 16.29 ± 2.41, P < 0.01). At 3 months after drug withdrawal, the abdominal circumference was significantly smaller in group A than in B and C (ï¼»78.10 ± 6.00ï¼½ vs ï¼»84.15 ± 8.17ï¼½ and ï¼»91.53 ± 11.49ï¼½ cm, P < 0.01) and the HDL level lower in group C than in A and B (ï¼»0.96 ± 0.15ï¼½ vs ï¼»1.27 ± 0.14ï¼½ and ï¼»1.16 ± 0.2ï¼½] mmol/L, P < 0.01). Systolic blood pressure exhibited statistically significant differences between any two time points in group C (P < 0.05 or P < 0.01) but not in group A (P > 0.05) or B (P > 0.05). Diastolic blood pressure was markedly lower in group B right after medication and at 3 months after drug withdrawal than before treatment (ï¼»75.62 ± 10.70ï¼½ and ï¼»74.65 ± 9.90ï¼½ vs ï¼»78.00 ± 11.42ï¼½ mmHg, P < 0.05), and so was it in group C (ï¼»82.19 ± 10.36ï¼½ and ï¼»82.40 ± 10.09ï¼½ vs ï¼»86.71 ± 12.32ï¼½ mmHg, P < 0.05), but manifested no significant difference between any two time points in group A (P > 0.05). There were statistically significant differences in the FBS level among different time points in groups A and C (P < 0.05) but not in B between post-treatment and 3 months after drug withdrawal (ï¼»5.34 ± 0.60ï¼½ vs ï¼»5.36 ± 0.40ï¼½ mmol/L, P > 0.05), and so were there in the TG level among different time points in groups A and C (P < 0.05) but not in B between pre- and post-treatment (ï¼»1.80 ± 0.98ï¼½ vs ï¼»1.64 ± 1.19ï¼½ mmol/L, P > 0.05). CONCLUSIONS: Periodic administration of low-dose sustained-release PDE5 inhibitors with health education and lifestyle guidance may reverse ED with SCMS and improve most of the related metabolic parameters.


Assuntos
Disfunção Erétil/tratamento farmacológico , Síndrome Metabólica/complicações , Inibidores da Fosfodiesterase 5/administração & dosagem , Tadalafila/administração & dosagem , Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Ereção Peniana , Adulto Jovem
4.
J Cell Sci ; 131(3)2018 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-29242228

RESUMO

Sepsis is an aggressive and life-threatening systemic inflammatory response with a high mortality. Inflammation and coagulation play crucial roles in the pathogenesis of sepsis in a mutually promoting manner. Unlike other single-target molecular therapies that have no obvious effects on clinical sepsis, bone marrow stromal cell (BMSC) therapy offers a broader spectrum of activities ranging from immune and inflammation suppression to tissue regeneration. In this report, we demonstrate that BMSC injection attenuates septic coagulopathy. It decreased the mortality, mitigated lung injury and reduced the surge of proinflammatory factors in mice with sepsis induced by cecal ligation and puncture (CLP). An in vitro cell model also revealed that co-culture with BMSCs reduced secretion of proinflammatory factors and injury of endothelial cells in response to lipopolysaccharide (LPS), an endotoxin of gram-negative bacteria. Together, our results demonstrate that BMSCs suppress sepsis-induced inflammation, endothelial dysfunction and defective coagulation.


Assuntos
Coagulação Sanguínea , Ceco/patologia , Inflamação/sangue , Inflamação/patologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Sepse/etiologia , Sepse/terapia , Animais , Coagulação Sanguínea/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Mediadores da Inflamação/metabolismo , Ligadura , Lipopolissacarídeos/farmacologia , Pulmão/patologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Masculino , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Modelos Biológicos , Punções , Sepse/sangue
5.
Oncol Lett ; 14(2): 1536-1542, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28789377

RESUMO

CKLF-like MARVEL transmembrane domain-containing 5 (CMTM5) has been reported to function as a potential tumor suppressor in several human cancers. However, the involvement of CMTM5 in human renal cell carcinoma (RCC) remains unclear. The current study aimed to detect its expression pattern in RCC tissues and cells, and to determine its anti-proliferative functions in this malignancy. The mRNA and protein expression levels of CMTM5 in RCC tissues and cells were detected by reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting. Following the transfection with CMTM5 lentivirus or control lenti-EGFP lentivirus into the RCC cell line ACHN, the viability, migration, apoptosis and cell cycle of these cells were detected by Cell Counting kit-8 assay, Transwell assay and flow cytometry, respectively. Compared with the adjacent non-malignant kidney tissue samples, CMTM5 expression was significantly downregulated in RCC tissues (P<0.05). In addition, enforced expression of CMTM5 could efficiently inhibit the cell growth of ACHN cells, which were arrested in G0/G1 phase. Furthermore, the migration and invasion of ACHN cells were also inhibited by restoration of CMTM5 expression. The present data suggest that CMTM5 may function as a tumor suppressor in human RCC by suppressing the viability of RCC cells, implying its potential as a therapeutic target for this malignancy.

6.
Zhonghua Nan Ke Xue ; 21(3): 214-8, 2015 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-25898551

RESUMO

OBJECTIVE: To investigate the protective effect of phosphodiesterase type 5 inhibitors (tadalafil) on the testis following testicular ischemia-reperfusion injury in rats. METHODS: Eighty-four healthy adult male SD rats were randomly and equally divided into groups A (sham operation), B (testicular torsion + low-dose tadalafil), C (testicular torsion + high-dose tadalafil), and D (testicular torsion + placebo). Models were established in the latter three groups by 7200 torsion of the right testis for 2 hours. The animals in groups A and B were treated by gavage with tadalafil at the dose of 0. 5 mg per kg per day, those in group C at 2 mg per kg per day, and those in group D with saline at the same dose. After 3, 7, and 14 days of treatment, the torsioned testes were harvested for evaluation of the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the testis tissue. The pathological changes in the testis were observed under the light microscope. RESULTS: At 3, 7, and 14 days, the SOD activity was (254.46 +/- 7.43), (278.49 +/- 8.33), and (317.99 +/- 3.31) nU/mg prot in group B, and (277.12 +/- 8.80), (309.40 +/- 2.14), and (320.39 +/- 4.72) nU/mg prot in group C, all obviously higher than in D ([223.21 +/- 4.65], [231.45 +/- 4.16] and [248.28 +/- 5.74] nU/mg prot), while the MDA content was lower in the former two groups than in the latter. At 3 and 7 days, the SOD activity was significantly higher and the MDA level significantly lower in group C than in B (both P < 0.01) , while at 14 days, neither showed any remarkable differences between the two groups (P > 0.05). No obvious histopathological change was observed in the testis tissue of group A. At 3 and 7 days, pathological examination of the testis tissue revealed significant differences in the number of seminiferous epithelial layers, testicular histological score, and seminiferous tubule diameter in group B (P < 0.01), but the three indexes at 14 days in group B and at 7 days in group C exhibited no remarkable differences from those at 14 days in group A. CONCLUSION: Tadalafil can alleviate testicular ischemia-reperfusion injury following testis torsion/detorsion in a time- and dose-dependent manner.


Assuntos
Carbolinas/farmacologia , Inibidores da Fosfodiesterase 5/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Testículo/irrigação sanguínea , Animais , Biomarcadores/metabolismo , Carbolinas/administração & dosagem , Relação Dose-Resposta a Droga , Masculino , Malondialdeído/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Túbulos Seminíferos/patologia , Torção do Cordão Espermático/complicações , Superóxido Dismutase/metabolismo , Tadalafila , Testículo/metabolismo , Testículo/patologia , Fatores de Tempo
7.
Emerg Med Australas ; 26(6): 538-42, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25319720

RESUMO

BACKGROUND: Chinese physicians are not only facing heavy work overloads, but also abuse and injury because of patient mistrust of physicians. The primary objective of the present study was to measure psychological distress, burnout levels and job satisfaction among Chinese emergency physicians. METHODS: All the physicians from the EDs of three large general hospitals were recruited to undertake a questionnaire-based survey from March to April 2012. The Hospital Anxiety and Depression Scale (HADS), Maslach Burnout Inventory-General Survey and Minnesota Satisfaction Questionnaire were used. Correlations between job satisfaction and psychological distress and burnout were calculated using the Pearson correlation. An outcome was considered statistically significant if P < 0.05. RESULTS: Completed questionnaires were received from 205 (82.0%) physicians. The mean HADS anxiety subscale scores for the ED physicians and general population were 7.8 ± 3.4 and 4.7 ± 3.5, respectively (t = 1.526, P < 0.05). Additionally, the mean HADS depression subscale scores were 7.9 ± 3.6 and 4.7 ± 3.9, respectively (t = 1.567, P < 0.05). Fifty-two (25.4%) exhibited high levels of career burnout. All aspects of job satisfaction were significantly lower in the ED physicians compared with a previous report (P < 0.05). Burnout was significantly negatively correlated with intrinsic and extrinsic job satisfaction in the sampled population. CONCLUSION: Psychological distress is prevalent in this group of ED physicians, and it deserves attention from the whole society. Burnout and job satisfaction among ED physicians are at a 'moderate' level. Burnout is negatively associated with higher job satisfaction.


Assuntos
Esgotamento Profissional/psicologia , Medicina de Emergência , Serviço Hospitalar de Emergência/estatística & dados numéricos , Satisfação no Emprego , Estresse Psicológico/epidemiologia , Adulto , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Escalas de Graduação Psiquiátrica , Estresse Psicológico/etiologia , Inquéritos e Questionários , Carga de Trabalho
8.
Beijing Da Xue Xue Bao Yi Xue Ban ; 45(3): 448-51, 2013 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-23774926

RESUMO

OBJECTIVE: To investigate the role of glycoprotein non-metastatic melanoma protein B (GPNMB) in renal cell carcinoma (RCC). METHODS: The method of immunohistochemistry (IHC) and Western blot were utilized to examine the expression of GPNMB in RCC and the normal adjacent tissues matched. RESULTS: The expression of GPNMB was lower in RCC than in the matched normal adjacent tissues (P=0.022). CONCLUSION: The abnormal expression of GPNMB may play an important role in the development of RCC and the detection of GPNMB may be useful for the early diagnosis of tumor and its development.


Assuntos
Carcinoma de Células Renais/metabolismo , Glicoproteínas de Membrana/metabolismo , Carcinoma de Células Renais/genética , Humanos , Imuno-Histoquímica , Glicoproteínas de Membrana/genética
9.
Mol Med Rep ; 8(2): 419-24, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23783575

RESUMO

Integrin-linked kinase (ILK) localizes at focal adhesion sites, plays an important role in cell-matrix interactions and is involved in the regulation of tumor cell growth and migration. The aim of the present study was to clarify the functional characterization of ILK in prostate cancer (PCa) cells. ILK was shown to be overexpressed in 57.1% (36/63) of PCa samples and 18.2% (2/11) of benign prostatic hyperplasia (BPH) samples using immunohistochemical analysis. DU145 PCa cells knocked down for ILK were examined using western blot analysis, proliferation assay, flow cytometry and wound healing assay. Depletion of ILK significantly impaired cell growth and motility, induced apoptosis in vitro, and delayed xenograft tumor proliferation in nude mice, which were important for oncogenesis and tumor progression. Western blot analysis showed that Akt activity was attenuated in ILK­depleted cells compared with the control cells. These results indicate that ILK knockdown attenuates the biological behavior of PCa cells by decreasing Akt activity, demonstrating that ILK is involved in the development and progression of PCa. Thus, ILK is suggested to serve as a potential therapeutic target for PCa.


Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias da Próstata/genética , Proteínas Serina-Treonina Quinases/genética , RNA Interferente Pequeno/genética , Animais , Apoptose/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/metabolismo , Modelos Animais de Doenças , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos , Gradação de Tumores , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Transdução de Sinais , Carga Tumoral/genética , Ensaios Antitumorais Modelo de Xenoenxerto
10.
Zhonghua Nan Ke Xue ; 19(3): 210-3, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23700724

RESUMO

OBJECTIVE: To observe the effects of CMTM2 on cyclophosphamide (CP)-induced reproductive toxicity and the expression of steroidogenic acute regulatory (StAR) protein in the transgenic mouse model. METHODS: Twenty CMTM2 transgenic mice were equally divided into a CMTM2 + CP and a CMTM2 + NS group, the former intraperitoneally injected with CP at 50 mg per kg per d, while the latter with the equivalent dose of normal saline, both for 7 days. Another 20 wild C57BL/6J mice were randomly assigned to a WT + CP and a WT + NS group, treated the same way above. After 30 days, all the mice were sacrificed and their epididymides and testes removed for measurement of the serum testosterone level by radioimmunoassay, determination of sperm concentration and motility by light microscopy and detection of the expression of StAR by Western blot. RESULTS: The levels of serum testosterone, sperm concentration and sperm motility were significantly decreased in the CMTM2 + CP group as compared with the CMTM2 + NS group ([42.98 +/- 3.25] nmol/L vs [46.74 +/- 3.38] nmol/L, [16.89 +/- 1.17 ] x 10(6)/ml vs [24.68 +/- 0.95 ] x 10(6)/ml, [72.75 +/- 1.25]% vs [85.14 +/- 1.12]%, P < 0.05), but remarkably less than in the WT + CP group ([37.97 +/- 4.17] nmol/L, [12.75 +/- 1.02] x 10(6)/ml, [50.52 +/- 1.37] %) (P < 0.05). However, the expression of StAR was significantly higher in the CMTM2 + CP than in the WT + CP group (1.16 +/- 0.07 vs 0.69 +/- 0.08, P < 0.05). CONCLUSION: CMTM2 antagonizes cyclophosphamide-induced reproductive toxicity via regulating the expression of StAR, and hence plays a protective role in the reproductive system.


Assuntos
Ciclofosfamida/toxicidade , Proteínas com Domínio MARVEL/genética , Proteínas Repressoras/genética , Testículo/metabolismo , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Contagem de Espermatozoides , Motilidade Espermática , Testículo/efeitos dos fármacos
11.
Beijing Da Xue Xue Bao Yi Xue Ban ; 45(2): 217-20, 2013 Apr 18.
Artigo em Chinês | MEDLINE | ID: mdl-23591340

RESUMO

OBJECTIVE: To investigate the effects of free fatty acids on cell proliferation and integrin-linked kinase (ILK) expression in human renal carcinoma 786-O cell line. METHODS: The 786-O cells were exposed to normal medium and different concentrations of oleic acid (OA) carried by de-fatty bovine serum albumin (d-BSA). The MTT assay and the flow cytometry assay were performed respectively for cell proliferation and apoptosis after the treatment with OA for 48 h. The expressions of ILK, Akt and p-Akt were detected by Western blot. RESULTS: The MTT assay showed that the cell viabilities of 0.05 mmol/L, 0.1 mmol/L and 0.2 mmol/L OA groups were increased gradually, as compared with the blank control (absorbance: 0.657 ± 0.056, 0.682 ± 0.028, 0.718 ± 0.042 vs. 0.495 ± 0.034; all P<0.001). The effects of OA on cells apoptosis were not significant (apoptotic rates: 2.42% ± 0.25% vs. 2.33% ± 0.87% vs. 2.25%± 0.51%, P=0.082). After being treated with OA, the expressions of ILK and p-Akt were increased in 786-O cells. CONCLUSION: The results suggested that free fatty acids could promote the development of renal cell carcinoma via up-regulating ILK/Akt pathway, which may reveal the relations between metabolic disturbance and renal carcinoma to a certain extent.


Assuntos
Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ácidos Graxos não Esterificados/farmacologia , Neoplasias Renais/patologia , Proteínas Serina-Treonina Quinases/metabolismo , Linhagem Celular Tumoral , Humanos , Neoplasias Renais/enzimologia , Proteínas Serina-Treonina Quinases/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Regulação para Cima
12.
Oncol Lett ; 5(4): 1395-1399, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23599801

RESUMO

An increased risk of renal cell carcinoma (RCC) has been linked with obesity and metabolic syndrome. However, the mechanisms by which lipid metabolic disorders affect the development of RCC remain unclear and highly controversial. Integrin-linked kinase (ILK) is a serine/threonine protein kinase involved in the regulation of tumor cell growth and angiogenesis. In the present study, the effect of free fatty acids in the promotion of RCC progression was investigated by upregulating ILK. Results of the MTT assay indicated that treatment of 786-O cells with oleic acid induced a concentration-dependent increase in cell viability. Flow cytometry analysis revealed that the effect of oleic acid on cell apoptosis was not significant. Following treatment with oleic acid, the expression of ILK, phospho-Akt and G protein-coupled receptor 40 (GPR40) was increased in 786-O cells. These effects were reversed when the expression of ILK was downregulated using specific small interfering RNA. These results indicate that free fatty acids are associated with the development of renal cell carcinoma via activation of the GPR40/ILK/Akt pathway, revealing a novel mechanism for the correlation between metabolic disturbance and renal carcinoma.

13.
PLoS One ; 8(3): e59796, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23536888

RESUMO

Abnormal serum lipid profiles are associated with the risk of some cancers, but the direction and magnitude of the association with renal cell carcinoma is unclear. We explore the relationship between serum lipids and renal cell carcinoma via a matched case-control study. A 1∶2-matched case-control study design was applied, where one renal cell carcinoma patient was matched to two non-renal-cell-carcinoma residents with respect to age (±0 year) and gender. Cases (n = 248) were inpatients with a primary diagnosis of renal cell carcinoma, confirmed by pathology after operations. Controls were sampled from a community survey database matched on age and gender with cases, 2 controls for each case. Stratified Cox proportional hazard regression analysis was used to obtain hazard ratios and corresponding 95% confidence intervals of lipids level and dyslipidemia for the risk of renal cell carcinoma. Elevated serum cholesterol (p<0.001), LDL cholesterol (p<0.001), and HDL cholesterol (p = 0.003) are associated with decreased hazard of renal cell carcinoma, adjusting for obesity, smoke, hypertension and diabetes. However, risk caused by hTG showed no statistical significance (p = 0.263). This study indicates that abnormal lipid profile influences the risk of renal cell carcinoma.


Assuntos
Carcinoma de Células Renais/complicações , Dislipidemias/complicações , Neoplasias Renais/complicações , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Humanos , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Estadiamento de Neoplasias , Razão de Chances , Fatores de Risco , Adulto Jovem
14.
Zhonghua Nan Ke Xue ; 18(6): 483-6, 2012 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-22774599

RESUMO

OBJECTIVE: To establish a transgenic mouse model systemically expressing the CMTM2 gene and study the effect of the CMTM2 expression on the reproductive system of mice in vivo. METHODS: Transgenic mice were generated by microinjection of pRevTRE-CMTM2 and the genotype was detected by PCR. The expression of CMTM2 was determined by RT-PCR, Western blot and immunohistochemistry, and the serum testosterone level was measured by radioimmunoassay. RESULTS: The CMTM2 gene was highly expressed in the testis of the transgenic mouse models and in their offspring as well. The level of serum testosterone was significantly increased in the transgenic models as compared with the wild-type mice ([46.04 +/- 3.72] vs [42.43 +/- 3.80] nmol/L, P < 0.05). CONCLUSION: The transgenic mouse model was established successfully, which could highly express the CMTM2 gene. It is indicated that CMTM2 may influence steroidogenesis and testosterone secretion in transgenic mice.


Assuntos
Proteínas com Domínio MARVEL/genética , Camundongos Transgênicos , Testosterona/sangue , Animais , Genótipo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos ICR
15.
Zhonghua Nan Ke Xue ; 18(3): 195-9, 2012 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-22474980

RESUMO

OBJECTIVE: To investigate the inhibitory effect of CKLF-like MARVEL transmembrane domain containing 5 (CMTM5) on xenografted human prostatic cancer in nude mice and its action mechanism. METHODS: We established a model of xenografted prostatic cancer by inoculating PC-3 cells subcutaneously into nude mice, and 3 weeks later injected CMTM5 adenovirus locally into the tumor followed by daily observation of the tumor volume and body weight of the experimental animals. All the rats were killed 2 weeks after CMTM5 injection and the tumor tissue harvested for detection of the inhibitory effect of CMTM5 on the expressions of VEGF and NF-kappaB proteins by immunohistochemistry. RESULTS: The tumor volume was significantly smaller and body weight of the CMTM5-treated mice were (573.39 +/- 175.24) mm3 and (0.55 +/- 0.11) g, respectively, significantly decreased as compared with those of the controls ([1482.50 +/- 327.86] mm3 and [1.31 +/- 0.29] g) (P = 0.03 and P = 0.027). Immunohistochemistry showed that the expressions of VEGF and NF-kappaB were obviously down-regulated in the CMTM5 group in comparison with the control group. CONCLUSION: CMTM5 suppresses the growth of prostate cancer by down-regulating the expressions of VEGF and NF-kappaB.


Assuntos
Quimiocinas/farmacologia , Proteínas com Domínio MARVEL/farmacologia , Neoplasias da Próstata/metabolismo , Proteínas Supressoras de Tumor/farmacologia , Adenoviridae/genética , Animais , Linhagem Celular Tumoral , Quimiocinas/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Proteínas com Domínio MARVEL/genética , Masculino , Camundongos , Camundongos Nus , NF-kappa B/metabolismo , Proteínas Supressoras de Tumor/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
16.
Urology ; 79(6): 1385-8, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22482874

RESUMO

OBJECTIVE: To tentatively evaluate the usefulness of self-management interventions in improving the lower urinary tract symptoms (LUTS) and quality of life (QoL) in patients with benign prostatic hyperplasia (BPH). METHODS: Two-hundred twent-two men were recruited from a teaching hospital at Peking University from March 2008 to September 2009. They were referred by general practitioners to urologic outpatient departments because of BPH after 3-month administration of α-blockers. Participants were randomized to attend either a self-management program or undergo standard care. Difference of scores of International Prostate Symptom Score (IPSS) and BPH-specific QoL scale between 2 groups was analyzed at the enrollment period, and at the first week, third month, and sixth month. RESULTS: All participants had been followed for 6 months. There was no significant difference in IPSS score and QoL score between the 2 groups at the enrollment period and first week, whereas at the third month and sixth month, the IPSS scores and QoL self-management interventional group scores were statistically significant lower than those of the standard care group. CONCLUSION: Self-management intervention may be associated with decreased LUTS symptoms and may improve QoL in BPH patients with α-blocker administration.


Assuntos
Sintomas do Trato Urinário Inferior/terapia , Hiperplasia Prostática/terapia , Qualidade de Vida , Autocuidado , Conduta Expectante , Idoso , Comportamentos Relacionados com a Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Educação de Pacientes como Assunto
17.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 34(6): 625-8, 2012 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-23286412

RESUMO

CKLF-like MARVEL transmembrane domain containing member(CMTM)is a novel generic family firstly reported by Peking University Center for Human Disease Genomics. CMTM5 belongs to this family and has exhibited tumor-inhibiting activities. It can encode proteins approaching to the transmembrane 4 superfamily(TM4SF). CMTM5 is broadly expressed in normal adult and fetal human tissues, but is undetectable or down-regulated in most carcinoma cell lines and tissues. Restoration of CMTM5 may inhibit the proliferation, migration, and invasion of carcinoma cells. Although the exact mechanism of its anti-tumor activity remains unclear, CMTM5 may be involved in various signaling pathways governing the occurrence and development of tumors. CMTM5 may be a new target in the gene therapies for tumors, while further studies on CMTM5 and its anti-tumor mechanisms are warranted.


Assuntos
Quimiocinas , Proteínas com Domínio MARVEL , Proteínas Supressoras de Tumor , Quimiocinas/genética , Quimiocinas/metabolismo , Humanos , Proteínas com Domínio MARVEL/genética , Proteínas com Domínio MARVEL/metabolismo , Neoplasias/genética , Neoplasias/metabolismo , Transdução de Sinais , Proteínas Supressoras de Tumor/genética , Proteínas Supressoras de Tumor/metabolismo
18.
Beijing Da Xue Xue Bao Yi Xue Ban ; 43(4): 496-9, 2011 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-21844952

RESUMO

OBJECTIVE: To investigate the expression of glycoprotein non-metastatic melanoma protein B (GPNMB) in prostate cancer and its clinical significance. METHODS: The expression of GPNMB was analysed in 63 prostate cancer and 3 heterosexual hyperplasia prostate tissue and 8 benign prostatic hyperplasia samples by immunohistochemical staining, with integral optical density(IOD) value representing expression level of positive cells. RESULTS: The expression of GPNMB was lower in benign prostatic hyperplasia (BPH, IOD=70 017.49) than in Atypical hyperplasia (IOD=101 547.33, P=0.000 1) . The expression of GPNMB in tumor (IOD= 162 027.54) was higher than in non-tumor group (IOD=79 290.97), which included BPH and atypical hyperplasia (P=0.000 1). But GPNMB expression level was not positively elevated with degree of malignancy of prostate cancer. However, the expression of GPNMB in low pathological grading(IOD=177 944.30) was higher than that in high pathological grading(IOD=150 885.81, P=0.013). CONCLUSION: The abnormal expression of GPNMB may play an important role in the development of prostate cancer and its detection may be useful for the early diagnosis of prostate cancer.


Assuntos
Glicoproteínas de Membrana/metabolismo , Neoplasias da Próstata/metabolismo , Biomarcadores Tumorais/metabolismo , Humanos , Masculino , Próstata/metabolismo , Próstata/patologia , Hiperplasia Prostática/metabolismo , Neoplasias da Próstata/patologia
19.
Beijing Da Xue Xue Bao Yi Xue Ban ; 42(4): 386-90, 2010 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-20721248

RESUMO

OBJECTIVE: To discover whether there is any other pathway than EGFR which has interaction with HER2 that makes a different down stream on cancer manners and whether CMTM5 plays a role in HER2 related tumor performance. METHODS: HER2 and CMTM5 were detected on prostate cancer tissue chip using IHC. The protein levels of HER2, Cyclin D1 and CMTM5 were compared had with or without CMTM5 plasmid transfection on PC3 cell line to insure their relationship. RESULTS: The author demonstrated that the HER2 had been up regulated in prostate cancer epithelium while CMTM5 down regulated. Overexpression of CMTM5 in PC3 could lower the HER2 and Cyclin D1 protein level. CONCLUSION: The results suggest that in prostate cancer HER2 has a different partner in the signaling transduction other than EGFR as in a traditional pathway. CMTM5 may have a chance to be chosen as the next potential treatment bio-target of prostate cancer.


Assuntos
Quimiocinas/genética , Neoplasias da Próstata/genética , Receptor ErbB-2/genética , Proteínas Supressoras de Tumor/genética , Linhagem Celular Tumoral , Quimiocinas/metabolismo , Regulação para Baixo/genética , Receptores ErbB/genética , Receptores ErbB/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , Proteínas com Domínio MARVEL , Masculino , Neoplasias da Próstata/patologia , Receptor ErbB-2/metabolismo , Proteínas Supressoras de Tumor/metabolismo
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