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1.
J Cell Mol Med ; 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32065482

RESUMO

Glioblastoma multiforme (GBM) is a devastating brain tumour without effective treatment. Recent studies have shown that autophagy is a promising therapeutic strategy for GBM. Therefore, it is necessary to identify novel biomarkers associated with autophagy in GBM. In this study, we downloaded autophagy-related genes from Human Autophagy Database (HADb) and Gene Set Enrichment Analysis (GSEA) website. Least absolute shrinkage and selection operator (LASSO) regression and multivariate Cox regression analysis were performed to identify genes for constructing a risk signature. A nomogram was developed by integrating the risk signature with clinicopathological factors. Time-dependent receiver operating characteristic (ROC) curve and calibration plot were used to evaluate the efficiency of the prognostic model. Finally, four autophagy-related genes (DIRAS3, LGALS8, MAPK8 and STAM) were identified and were used for constructing a risk signature, which proved to be an independent risk factor for GBM patients. Furthermore, a nomogram was developed based on the risk signature and clinicopathological factors (IDH1 status, age and history of radiotherapy or chemotherapy). ROC curve and calibration plot suggested the nomogram could accurately predict 1-, 3- and 5-year survival rate of GBM patients. For function analysis, the risk signature was associated with apoptosis, necrosis, immunity, inflammation response and MAPK signalling pathway. In conclusion, the risk signature with 4 autophagy-related genes could serve as an independent prognostic factor for GBM patients. Moreover, we developed a nomogram based on the risk signature and clinical traits which was validated to perform better for predicting 1-, 3- and 5-year survival rate of GBM.

2.
FEBS Open Bio ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31984680

RESUMO

Colorectal cancer (CRC) is the third main cause of cancer-relevant deaths worldwide, and its incidence has increased in recent decades. Previous studies have indicated that certain long non-coding RNAs (lncRNAs) have regulatory roles in tumor occurrence and progression. Often, lncRNAs are competitive endogenous RNAs (ceRNAs) which sponge miRNAs to up-regulate mRNAs. Here, we examined the role of a novel lncRNA BBOX1 antisense RNA 1 (BBOX1-AS1) in colorectal cancer (CRC). We observed that BBOX1-AS1 is overexpressed in CRC cell lines, and BBOX1-AS1 knockdown enhances cell proliferation, migration and invasion while reducing cell apoptosis. MiR-361-3p is present at a low level in CRC and is negatively modified by BBOX1-AS1. Moreover, miR-361-3p was validated to be targeted by BBOX1-AS1. SH2B1 was notably up-regulated in CRC cell lines, and identified as a downstream gene of miR-361-3p. In addition, we found that miR-361-3p amplification can suppress the expression of SH2B1. Finally, data from rescue assays suggested that overexpression of SH2B1 counteracted BBOX1-AS1 silencing-mediated inhibition of CRC progression. In conclusion, BBOX1-AS1 promotes CRC progression by sponging hsa-miR-361-3p and up-regulating SH2B1.

3.
Chemosphere ; 247: 125812, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31972483

RESUMO

There emerges an urgent stipulation towards the enhanced toluene catalytic combustion nanocatalysts for whittling down the footprint of toluene, a notorious air pollutant. Unfortunately, Few materials which are currently made accessible both present the high catalytic performance lower than 250 °C and keep durable at elevated temperatures. Herein, we demonstrate an expeditious salt hydrolysis-driven redox-precipitation protocol wherein H+ donated by the hydrolysis of copper salt was used to initiate the regioselective reduction of KMnO4 by H2O2 under controlled redox kinetics in order to assemble the homogeneous mixed solid solution hollow microsphere Cu-Mn-based structure. Manifold characterization technologies unveil that in this unique nanbomicrosphere the abundant microscaled pores are successfully created across Cu-Mn bulks with fine-modulating the chemical properties. In sharp contrast with the compact counterparts without tailed porosity, the tuned crystallinity, accessed edge sites with the unsaturated coordination, fast redox chemistry, and boosted gaseous diffusion during reactions synergize to result in the signally good toluene oxidation, with the complete elimination activity at 252 °C, T90 at 237 °C, and prominent long-term durability under the stringent reaction atmospheres. Our current study ushers in an alternative and tractable arena to excogitate the porous oxide materials for multifarious catalysis implementations.

4.
Oral Dis ; 26(1): 122-130, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31710746

RESUMO

OBJECTIVE: We determined the bacterial community structure of the buccal mucosa in patients with oral lichen planus (OLP) and evaluated the potential association of Fusobacterium nucleatum with OLP. SUBJECTS AND METHODS: We collected buccal mucosal swab samples of patients with OLP (n = 20) and healthy controls (n = 10) and performed 16S rRNA gene sequencing and real-time PCR to determine potentially different bacteria. Damaged and adjacent non-damaged mucosal swab samples of 25 OLP patients were used to detect the amount of F. nucleatum by real-time PCR. RESULTS: Compared with healthy controls, enrichment of Fusobacterium and Granulicatella was more abundant in patients with OLP (p = .0146 and 0.0034). The abundance of Fusobacterium and F. nucleatum was significantly enriched on buccal mucosa of patients with OLP compared with healthy controls (p = .0043 and 0.0235). Compared with adjacent non-damaged buccal mucosa of OLP patients, the amount of F. nucleatum in the damaged mucosa was significantly increased (p = .001). We examined third-level KEGG pathways for bacteria on mucosal surface and found that genes controlling sporulation and ether lipid metabolism were enriched in patients with OLP. CONCLUSIONS: A high amount of F. nucleatum may be associated with OLP. Further studies are required to investigate the precise association of F. nucleatum with OLP.


Assuntos
Fusobacterium nucleatum/isolamento & purificação , Líquen Plano Bucal/microbiologia , Mucosa Bucal/microbiologia , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética
5.
J Cell Biochem ; 121(1): 25-42, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31433522

RESUMO

Myocardial dysfunction is an important manifestation of sepsis. In addition, inactivation of the mitogen-activated protein kinase (MAPK) signaling pathway has been reported to be beneficial in sepsis. The current study used gene expression profiling to demonstrate the overexpression of angiotensin II type 1 receptor (AT1R) and activation of the MAPK signaling pathway in sepsis. In this study, we used a rat model of sepsis established by cecal ligation and puncture to explore the mechanism of AT1R silencing in relation to the MAPK signaling pathway on myocardial injury. Various parameters including blood pressure, heart rate, and cardiac function changes were observed. Enzyme-linked immunosorbent assay was used to measure the concentration of cardiac troponin T (TnT), cardiac troponin I (cTnI), and creatine kinase isoenzyme muscle/brain (CK-MB). Myocardial enzyme, tissue antioxidant capacity, mitochondria swelling, and membrane potential were also detected. Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling staining was applied to measure cell apoptosis, and messenger RNA and protein levels of apoptosis-related proteins (Fas ligand [Fasl], B-cell CLL/lymphoma [Bcl-2], p53) were also detected. Initially, sepsis rats exhibited decreased survival rate, but increased ejection fraction (EF), heart rate, and concentrations of TnT, cTnI, and CK-MB. Furthermore, decreased AT1R expression inactivated the MAPK signaling pathway (shown as decreased extracellular signal-regulated kinase and cyclic adenosine 3',5'-monophosphate response element binding protein expression), decreased EF, heart rate, and concentrations of TnT, cTnI, and CK-MB, but increased sepsis rat survival rate. Eventually, decreased AT1R expression inhibited myocardial cell apoptosis (shown as decreased apoptosis rate and p53 and Fasl expression as well as increased Bcl-2 expression). These findings indicated that AT1R silencing plays an inhibitory role in sepsis-induced myocardial injury by inhibiting the MAPK signaling pathway.

6.
Fish Shellfish Immunol ; 97: 432-439, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31883470

RESUMO

As a high mortality disease, Infectious spleen and kidney necrosis virus (ISKNV) can cause massive economic damage on mandarin fish farming industry in China, which seriously hindered the development of mandarin fish farming industry. In this research, SWCNTs (single-walled carbon nanotubes) as a candidate for DNA vaccine carrier was vaccinated by immersion (1, 2, 5, 10, 20 mg/L) in juvenile mandarin fish. In muscle, spleen and kidney tissues, the results showed that transcription and expression of MCP gene can be detected in pcDNA-MCP and SWCNTs-pcDNA-MCP groups after bath immunization. The immune response (immune-related genes expression, serum antibody production, enzyme activities and C3 content) was significantly enhanced in fish which vaccinated with SWCNTs-pcDNA-MCP in comparison with those vaccinated with pcDNA-MCP alone. After 14 d challenge, the RPS (relative percentage survival) can be enhanced which using SWCNTs as a carrier in SWCNTs-pcDNA-MCP (82.4%) group at 20 mg/L (the highest vaccine dose) than the naked pcDNA-MCP (54.2%) group. This study reveals that functionalized SWCNTs could be a promising immersion DNA vaccine carrier in aquaculture.

7.
Front Oncol ; 9: 1059, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31681595

RESUMO

Background: Glioma is the most common primary brain tumor with a dismal prognosis. It is urgent to develop novel molecular biomarkers and conform to individualized schemes. Methods: Differentially expressed pseudogenes between low grade glioma (LGG) and glioblastoma multiforme (GBM) were identified in the training cohort. Least absolute shrinkage and selection operator (LASSO) regression and multivariate Cox proportional hazards regression analyses were used to select pseudogenes associated with prognosis of glioma. A risk signature was constructed based on the selected pseudogenes for predicting the survival of glioma patients. A pseudogene-miRNA-mRNA regulatory network was established and visualized using Cytoscape 3.5.1. Gene Oncology (GO) and signaling pathway analyses were performed on the targeted genes to investigate functional roles of the risk signature. Results: Five pseudogenes (ANXA2P2, EEF1A1P9, FER1L4, HILS1, and RAET1K) correlating with glioma survival were selected and used to establish a risk signature. Time-dependent receiver operating characteristic (ROC) curves revealed that the risk signature could accurately predict the 1, 3, and 5-year survival of glioma patients. GO and signaling pathway analyses showed that the risk signature was involved in regulation of proliferation, migration, angiogenesis, and apoptosis in glioma. Conclusions: In this study, a risk signature with five pseudogenes was constructed and shown to accurately predict 1-, 3-, and 5-year survival for glioma patient. The risk signature may serve as a potential target against glioma.

8.
Nanomaterials (Basel) ; 9(12)2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31766673

RESUMO

Supercapacitors have the rapid charge/discharge kinetics and long stability in comparison with various batteries yet undergo low energy density. Theoretically, square dependence of energy density upon voltage reveals a fruitful but challenging engineering tenet to address this long-standing problem by keeping a large voltage window in the compositionally/structurally fine-tuned electrode/electrolyte systems. Inspired by this, a facile salt-templating enables hierarchically porous biochars for supercapacitors filled by the high-voltage ionic liquids (ILs). Resultant nanostructures possess a coherent/interpenetrated framework of curved atom-thick sidewalls of 0.8-/1.5-nanometer pores to reconcile the pore-size-dependent adlayer structures of ILs in nanopores. Surprisingly, this narrow dual-model pore matches ionic radii of selected ILs to accommodate ions by unique coupled nano-/bi-layer nanoconfinements, augmenting the degree of confinement (DoC). The high DoC efficiently undermines the coulombic ordering networks and induces the local conformational oscillations, thus triggering an anomalous but robust charge separation. This novel bi-/mono-layer nanoconfinement combination mediates harmful overscreening/overcrowding effects to reinforce ion-partitioning, mitigating long-lasting conflicts of power/energy densities. This interesting result differs from a long-held viewpoint regarding the sieving effect that ion-in-pore capacitance peaks only if pore size critically approaches the ion dimension. Optimal biocarbon finally presents a very high/stable operational voltage up to 4 V and specific energy/power rating (88.3 Whkg-1 at 1 kWkg-1, 47.7 Whkg-1 albeit at a high battery-accessible specific power density of 20 kWkg-1), overwhelmingly outperforming most hitherto-reported supercapacitors and some batteries. Such attractive charge storage level can preliminarily elucidate an alternative form of a super-ionic-state high-energy storage linked with both the coordination number and coulombic periodism of the few ion-sized mesopores inside carbon electrodes, escalating supercapacitors into a novel criterion of charge delivery.

9.
J Mater Sci Mater Med ; 30(11): 123, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686219

RESUMO

Prognosis and treatment evaluation of spinal cord injury (SCI) are still in the long-term research stage. Prognostic factors for SCI treatment need effective biomarker to assess therapeutic effect. Quantitative diffusion tensor imaging (DTI) may become a potential indicators for assessing SCI repair. However, its correlation with the results of locomotor function recovery and tissue repair has not been carefully studied. The aim of this study was to use quantitative DTI to predict neurological repair of SCI with transplanting collagen/chitosan scaffold binding basic fibroblast growth factor (bFGF). To achieve our research goals, T10 complete transection SCI model was established. Then collagen/chitosan mixture adsorbed with bFGF (CCS/bFGF) were implanted into rats with SCI. At 8 weeks after modeling, implanting CCS/bFGF demonstrated more significant improvements in locomotor function according to Basso-Beattie-Bresnahan (BBB) score, inclined-grid climbing test, and electrophysiological examinations. DTI was carried out to evaluate the repair of axons by diffusion tensor tractgraphy (DTT), fractional anisotropy (FA) and apparent diffusion coefficient (ADC), a numerical measure of relative white matter from the rostral to the caudal. Parallel to locomotor function recovery, the CCS/bFGF group could significantly promote the regeneration of nerve fibers tracts according to DTT, magnetic resonance imaging (MRI), Bielschowsky's silver staining and immunofluorescence staining. Positive correlations between imaging and locomotor function or histology were found at all locations from the rostral to the caudal (P < 0.0001). These results demonstrated that DTI might be used as an effective predictor for evaluating neurological repair after SCI in experimental trails and clinical cases.

10.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(8): 951-956, 2019 Aug 28.
Artigo em Chinês | MEDLINE | ID: mdl-31570686

RESUMO

Benign metastasizing leiomyoma (BML) often occurs in women with a history of uterine leiomyoma at the childbearing age. At the same time, BML cases with multiple sites of metastatic lesions are extremely rare. A BML patient with multiple metastases of uterine leiomyoma in two lung and lumbar spine after surgery was admitted to Department of Spinal Surgery, Second Xiangya Hospital, Central South University in September 2017. Positron emission tomography/computed tomography (PET/CT) can perform a whole body examination for BML patients, which can find metastases in many parts of the body such as lung, abdominal cavity, pelvic cavity and spine. The PET/CT results are helpful to a comprehensive diagnosis. The imaging and clinical features of BML are now explored in combination with the characteristics of the case and relevant literature reports.


Assuntos
Leiomioma , Neoplasias Uterinas , Feminino , Humanos , Vértebras Lombares , Neoplasias Pulmonares , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons
11.
ACS Chem Neurosci ; 10(11): 4502-4510, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31642670

RESUMO

The Chinese mitten crab (Eriocheir sinensis) is a commercially important crab in China and is usually managed at high stocking densities. Agonistic behavior directly impacts crab integrity, survival, and growth and results in economic losses. In the present study, we evaluated the modulatory effects of serotonin (5-HT) and dopamine (DA) though the 5-HT2 and DA2 receptor-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway on agonistic behavior. The results showed that injection of either 10-6 mol/crab 5-HT or DA reduced the agonistic behavior of E. sinensis (P < 0.05), as did 10-10 mol/crab DA and 10-8 mol/crab 5-HT and DA (P < 0.05); however, a dose of 10-10 mol/crab 5-HT promoted agonistic behavior. 5-HT significantly increased the mRNA expression level of 5-HT7 receptor and reduced that of the DA2 receptor in the cerebral ganglion (P < 0.05). In contrast to 5-HT, DA significantly decreased 5-HT2B mRNA levels and increased 5-HT7 and DA2 receptor levels in the thoracic ganglia (P < 0.05). In addition, injections of either 5-HT or DA increased the cAMP and PKA levels in hemolymph (P < 0.05). By using in vitro culture of the thoracic ganglia, the current study showed that ketanserin (5-HT2 antagonist) and [R(-)-TNPA] (DA2 agonist) had obvious effects on the expression levels of the two receptors (P < 0.05). In vivo experiments further demonstrated that ketanserin and [R(-)-TNPA] could both significantly reduce the agonistic behavior of the crabs (P < 0.05). Furthermore, both ketanserin and [R(-)-TNPA] promoted the cAMP and PKA levels (P < 0.05). The injection of CPT-cAMP (cAMP analogue) elevated the PKA levels and inhibited agonistic behavior. In summary, this study showed that 5HT-2B and DA2 receptors were involved in the agonistic behavior that 5-HT/DA induced through the cAMP-PKA pathway in E. sinensis.

12.
Front Chem ; 7: 591, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508412

RESUMO

LiNi0.5Mn1.5O4 (LNMO) is a potential cathode material for lithium-ion batteries with outstanding energy density and high voltage plateau (>4.7 V). However, the interfacial side reaction between LNMO and the liquid electrolyte seriously causes capacity fading during cycling at the high voltage. Here, p-toluenesulfonyl isocyanate (PTSI) is used as the electrolyte additive to overcome the above problem of LNMO. The results show that the specific capacity of LNMO/Li cell with 0.5 wt.% PTSI at the first cycle is effectively enhanced by 36.0 mAh/g and has better cycling performance than that without PTSI at 4.98 V. Also, a stable solid electrolyte interface (SEI) film derived from PTSI is generated on the electrode surface, which could alleviate the strike of hydrofluoric acid (HF) caused by electrolyte decomposition. These results are explained by the molecular structure of PTSI, which contains SO3. The S=O groups can delocalize the nitrogen nucleus to block the reactivity of PF5.

13.
Zhongguo Gu Shang ; 32(8): 721-726, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31533383

RESUMO

OBJECTIVE: To explore expression of ß-catenin and NF-κB signaling pathway in synovial tissue of rats with different degrees of knee osteoarthritis (KOA). METHODS: Forty-eight SPF male rats weighed (200±20) g were randomly divided into three groups, namely model group (32 rats), sham operation group (8 rats) and control group (8 rats). KOA model rats were established by Hulth method, and 8 rats were killed at 2, 4, 8, 12 weeks respectively after modeling, in order to establish KOA model rats with moderate, early, mild and severe degree. Sham operation group was only cut off capsule of knee joint and suture to exclude interference factor, control group was untreated. Behavior, synovial hyperplasia and cartilage degeneration of rats among each group were observed. Expression of NF-κB and signaling pathway and ß-catenin in synovial tissue of rats were detected by real-time PCR. RESULTS: KOA rat model was successfully established, and synovial hyperplasia was observed in KOA model at mild and early degree, and then gradually decreased; while cartilage degeneration in the early moderate and severe KOA model was significantly expressed, and gradually aggravated with time. The results of PCR showed that expression of ß-catenin in 4-week group (8.57±0.46) and 8-week group (4.23±0.09) were higher than those in control group (P<0.05); expression of TLR-2 in 2-week group (12.04±4.02) and 4-week group (8.54±2.13) were higher than those in control group(P<0.05), and TLR-4 in 2-week group(5.04±0.93), 4-week group (3.29±0.58) and 8-week group (1.63±0.12) were higher than those in control group; expression of NF-κB was significantly higher in 2-week group (10.15±2.04), 4-week group (15.97±4.17), 8-week group (7.69±1.48) and 12-week group (6.70±1.58) than that in control group (P<0.05), and expression of IL-1ß was significantly higher in 4-week group (2.79±0.25) and 8-week group (2.46±0.32) than that of control group (P<0.05). CONCLUSIONS: On the RNA expression level, both of ß-catenin and NF-κB signaling pathways are involved in synovial inflammation in KOA model rats, and they play a regulatory role in expression of IL-1ß, degeneration of KOA.


Assuntos
Osteoartrite do Joelho , Animais , Inflamação , Masculino , NF-kappa B , Ratos , Transdução de Sinais , beta Catenina
14.
Front Chem ; 7: 555, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31448262

RESUMO

Rapidly-growing demand for wearable and flexible devices is boosting the development of flexible lithium ion batteries (LIBs). The exploitation of flexible electrodes with high mechanical properties and superior electrochemical performances has been a key challenge for the rapid practical application of flexible LIBs. Herein, a flexible composite electrode was prepared from the mixed solutions of Li[Li0.2Ni0.13Co0.13Mn0.54]O2 (LLOs), carbon nanotubes(CNTs), and nanofibrillated celluloses (NFCs) via a vacuum filtration method. The resulting LLOs/CNTs/NFCs electrode delivered an initial discharge capacity of 253 mAh g-1 at 0.1 C in the voltage range from 2.0 to 4.6 V, and retained a reversible capacity of 178 mAh g-1 with 83% capacity retention after 100 cycles at 1 C. The LLOs/CNTs/NFCs electrode exhibited excellent flexibility along with repeated bending in the bending test. The LLOs/CNTs/NFCs electrode after bending test remained a discharge capacity of 149 mAh g-1 after 100 cycles at 1 C, and the corresponding capacity retentions was 76%. The excellent electrochemical performance and high flexibility can be ascribed to the framework formed by CNTs with high conductivity and NFCs with good mechanical properties. The results imply that the as-fabricated electrode can be a promising candidate for the flexible LIBs.

15.
Physiol Behav ; 209: 112621, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31323296

RESUMO

Agonistic behaviour is common in an encounter between two crustaceans. It often causes limb disability and consumes a lot of energy, which is harmful for the growth and survival of commercially important crustaceans. In the present study, we mainly focused on the agonistic behaviour of the Chinese mitten crab, Eriocheir sinensis, which is an important species of the aquaculture industry in China. We recorded agnostic behaviour with a high-definition camera and preliminarily evaluated the role of serotonin (5-HT) or dopamine (DA)-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway and eyestalk in the behaviour. The results showed that agonistic behaviour in E. sinensis consisted of three stages: approach, contact and fight. We found that the number of fights and cumulative time of fight were significantly higher in the male vs. male group than in the female vs. female and female vs. male groups (P < 0.05). After 1 h of agonistic behaviour, 5-HT concentration showed a significant increase and DA concentration showed a significant decrease when compared with the control group (no encounter; P < 0.05). 5-HT1B and 5-HT2B mRNA levels showed a significant increase in the eyestalk (P < 0.05). 5-HT7 mRNA levels showed significant downregulation in the thoracic ganglia and DA1A mRNA levels showed upregulation in the intestine (P < 0.05). DA2 mRNA levels showed a significant decrease in the eyestalk (P < 0.05). These changes were accompanied by a significant increase in cAMP level and significant decrease in PKA level in the haemolymph (P < 0.05). In addition, a significant decrease in glucose levels was detected after the agonistic behaviour. Crustacean hyperglycemic hormone (CHH) mRNA levels showed significant upregulation in the eyestalk and significant downregulation in the intestine (P < 0.05). The number of fights and cumulative time of fight in the left eyestalk ablation (L-X vs. L-X) group were more and longer than those in the intact eyestalk (C vs. C), right eyestalk ablation (R-X vs. R-X) and bilateral eyestalk ablation (D-X vs. D-X) groups. In short, E. sinensis shows special agonistic behaviour modulated by 5-HT or DA-cAMP-PKA pathway and eyestalk, especially the left eyestalk.

16.
Chin J Dent Res ; 22(2): 131-137, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31172141

RESUMO

OBJECTIVE: To evaluate the effect of premature delivery and birth weight (BW) on primary tooth eruption. METHODS: A total of 2,230 children aged 3 to 36 months from urban and rural areas in Beijing, China, were classified for analysis by gestational age at delivery (89 preterm and 2,141 full term) and BW (low, normal and high). The tooth eruption status of these children was examined and recorded every 3 months. RESULTS: The timing of first primary tooth eruption was significantly delayed in preterm infants (8.4 months versus 7.3 months for full term; P < 0.05). Furthermore, the number of teeth was significantly less for the preterm and low-BW groups at 12 to 18 and 24 to 30 months, but the number of teeth caught up with normal-BW children by 30 to 36 months. In contrast, the time of first tooth eruption of high-BW children was earlier, while the erupted teeth at each month range was more than the normal-BW group. This research also demonstrated a negative correlation (r = -0.202; P = 0.009) between the time of first primary tooth eruption and BW as well as a positive correlation between the number of erupted teeth and BW. CONCLUSION: Premature delivery and BW were the influencing factors for the timing of primary tooth eruption in children from Beijing, China. Recommendations for feeding habits and oral healthcare implementation may vary according to different primary tooth eruption status among individuals.


Assuntos
Recém-Nascido Prematuro , Erupção Dentária , Pequim , Peso ao Nascer , Criança , Pré-Escolar , China , Humanos , Lactente , Recém-Nascido , Dente Decíduo
17.
Fish Shellfish Immunol ; 92: 133-140, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31173860

RESUMO

Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which lead to significant economic loss on mandarin fish in China. There is no effective drug or vaccine against this fatal disease at present. Meanwhile, many drugs and vaccines had no effect in many cases account of several impenetrable barriers (cell, skin and gastrointestinal tract). Here we reported an immersion subunit vaccine system (SWCNTs-MCP) encoding MCP gene of ISKNV based on single-walled carbon nanotubes (SWCNTs). To evaluate its efficacy against ISKNV, we found a stronger and longer duration immune response (serum antibody production, enzyme activities and immune-related genes expression) can be induced in fish vaccinated with SWCNTs-MCP in comparison with those vaccinated with MCP alone. Importantly, SWCNTs can increase the immune protective effect of naked subunit vaccine by ca. 23.8%. Thereby, this study demonstrates that SWCNTs as a promising carrier for subunit vaccine might be used to vaccinate large-scale juvenile mandarin fish by bath administration approach.


Assuntos
Doenças dos Peixes/imunologia , Imunidade Inata , Iridoviridae/imunologia , Nanotubos de Carbono , Perciformes/imunologia , Vacinação/veterinária , Vacinas Virais/imunologia , Animais , Infecções por Vírus de DNA/imunologia , Infecções por Vírus de DNA/veterinária , Vacinas de Subunidades/imunologia
18.
Int J Neurosci ; 129(9): 842-847, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30724645

RESUMO

Purpose: To identify the regularity of signal evolution of intracerebral hemorrhage on susceptibility-weighted imaging (SWI) at different stages compared with T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI). Methods: We retrospectively evaluated a series of 365 patients who underwent T1WI, T2WI, and SWI examination simultaneously or sequentially in our hospital from January 2015 to May 2017. Two neuroradiologists assessed the images and discrepancies between their interpretations were resolved by consensus. Statistical analysis was performed using Chi-squared and Kappa tests. Results: Of the 365 patients on SWI sequence, 94 were enrolled. SWI detected the cases at different stages; T1WI detected 89 cases and T2WI detected 91 cases. The signal intensity of intracerebral hemorrhage on SWI was significantly associated with T1WI imaging and T2WI (χ2 = 4.651; p < 0.05; χ2 = 26.396; p < 0.01, respectively), especially at the late subacute stage. There was moderate consistency between the signal intensity of intracerebral hemorrhage on T2WI and SWI (Kappa coefficient = 0.530). Conclusion: Intracerebral hemorrhage has a varied appearance on SWI, and the evolution of signal of intracerebral hemorrhage on SWI sequence is influenced by T1WI and T2WI. Hematoma detection should be closely combined with clinical manifestation.


Assuntos
Hemorragia Cerebral/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Hemorragia Cerebral/epidemiologia , Feminino , Humanos , Masculino , Estudos Retrospectivos
19.
J Neurooncol ; 141(1): 19-30, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30539341

RESUMO

BACKGROUND: Glioblastoma (GBM) is the most common malignant tumor originating in the brain parenchyma. The invasive and infiltrative properties of glioblastoma result in poor clinical prognosis to conventional therapies. Emerging reports on microRNAs as important regulators during the process of EMT provide new insights into treating glioblastoma through new targets. However, underlying molecular mechanism of the regulation of miR-101-3p in glioblastoma remains unclear. METHODS: Level of miR-101-3p was determined in GBM cell lines by qRT-PCR. MTT, colony formation and transwell assays were utilized to evaluate functions of overexpression of miR-101-3p/knock down of TRIM44 on proliferation, migration and invasion in GBM cells. Direct interaction between miR-101-3p and TRIM44 was validated using dual luciferase reporter system and impacts of overexpression of miR-101-3p/knock down of TRIM44 on regulation of EMT markers were assessed by Western blotting. RESULTS: MiR-101-3p was validated to be repressed expressed in glioblastoma cancer cell lines. Both overexpression of miR-101-3p and knock down of TRIM44 attenuated proliferation, migration and invasion of glioblastoma cell lines in vitro. TRIM44 was shown to promote EMT in GBM progress and reverse inhibitory function of miR-101-3p. MiR-101-3p was found to suppress the expression of TRIM44 via directly targeting its 3'UTR. CONCLUSIONS: Our findings suggested miR-101-3p regulated proliferation and migration of glioblastoma cells through attenuating TRIM44 induced EMT via direct targeting 3'UTR of TRIM44, which provided preliminary study of potential therapeutic target in future GBM treatment.


Assuntos
Neoplasias Encefálicas/metabolismo , Proteínas de Transporte/metabolismo , Proliferação de Células , Glioblastoma/metabolismo , MicroRNAs/metabolismo , Metástase Neoplásica , Linhagem Celular Tumoral , Transição Epitelial-Mesenquimal , Regulação Neoplásica da Expressão Gênica , Humanos
20.
PLoS One ; 13(12): e0209617, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30586462

RESUMO

During pond culture of Eriocheir sinensis, a high limb-impairment rate restricts the industry development and quality. Therefore, research on limb autotomy and regeneration has important practical significance for the industrial development and basic biology of E. sinensis. This study evaluated the changes in bud morphology, growth-related gene expression and nutritional status during cheliped regeneration in E. sinensis. The study found that the new cheliped was pre-formed in the bud and then regenerated with the completion of molting of E. sinensis. The new cheliped was similar in morphology to the normal cheliped after the first molting but smaller in size. The qRT-PCR results of growth-related genes showed that the expression levels of EcR-mRNA (ecdysteroid receptor) and Chi-mRNA (chitinase) were significantly up-regulated, whereas the expression of MIH-mRNA (molt-inhibiting hormone) was significantly down-regulated (P < 0.05). The nutritional status during the regeneration process showed that the hepatopancreas total lipid content decreased significantly within 28 days and was significantly lower in the autotomy group than in the control group at 14 d and 21 d (P < 0.05). The hepatopancreas fatty acid composition results showed that saturated fatty acids (SFA), highly unsaturated fatty acids (HUFA) and n-3/n-6 were significantly higher in the autotomy group than in the control group at 21 d (P < 0.05), whereas the ∑ n-6 PUFA and ∑ n-3 PUFA at 1 d and 7 d, and the monounsaturated fatty acid (MUFA) at 28 d in the autotomy group were significantly lower than in the control group (P < 0.05). Moreover, the levels of eicosatetraenoic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) showed that DHA was significantly lower at 7 d and significantly higher at 21 d in the autotomy group than in the control group (P < 0.05), whereas ARA and EPA were not significantly different between the two groups. Muscle L-tryptophan content was significantly lower at 1 d and significantly higher at 7 d in the autotomy group than in the control group (P < 0.05). These results indicate that during the cheliped regeneration process, crabs could accelerate molting and regeneration by regulating growth-related gene expression (e.g., EcR-mRNA and MIH-mRNA) and nutrient metabolism (e.g., lipid metabolism).


Assuntos
Braquiúros/crescimento & desenvolvimento , Muda/genética , Estado Nutricional/genética , Regeneração/genética , Animais , Braquiúros/genética , Quitinases/genética , Extremidades/crescimento & desenvolvimento , Ácidos Graxos/metabolismo , Ácidos Graxos Insaturados/metabolismo , Hormônios de Invertebrado/genética , RNA Mensageiro/genética , Receptores de Esteroides/genética , Regeneração/fisiologia , Alimentos Marinhos
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