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1.
Artigo em Chinês | MEDLINE | ID: mdl-34365756

RESUMO

Objective: To study the effect of occupational exposure to non-steady state noise on hearing loss in the general equipment manufacturing industry, and to explore the feasibility of applying kurtosis index to evaluate hearing loss caused by non-steady state noise. Methods: A total of 233 workers exposed to non-steady state noise in 6 general equipment manufacturing enterprises were selected as the observation group, and 237 workers exposed to steady noise in 4 textile enterprises were selected as the control group between 2012 and 2018. Personal normalized continuous A-weighted sound pressure level equivalent to an 8 h-working-day (L(Aeq, 8 h)) was determined by a noise dosimeter. Cumulation noise exposure (CNE) was calculated from L(Aeq, 8 h) and related working age, and the CNE was adjusted by using noise kurtosis (CNE' after adjustment) . Meanwhile, questionnaires and hearing loss tests were performed for all subjects. The changes in the threshold of high-frequency hearing and the incidence of high-frequency hearing loss caused by noise in the two groups were compared before and after the adjustment of the kurtosis of CNE. The correlation between CNE and the incidence of high-frequency hearing loss before and after kurtosis adjustment was analyzed. Results: There was an difference between the 3000-8000 Hz hearing threshold of workers in different CNE groups (P<0.05) . logistic regression analysis showed that CNE was a risk factor of high-frequency hearing loss for workers in the observation group (OR=1.189, P<0.05) , and trend Chi-square test showed that the incidence of high-frequency hearing loss increased with CNE levels (χ(2)(trend)=34.415, P<0.05) .Before the kurtosis adjustment, in the CNE 95~<110 dB (A) ·year level group, the incidence of high-frequency hearing loss and the high-frequency hearing threshold in the observation group were significantly higher than those in the control group (P<0.05) . After kurtosis adjustment, there was no significant difference in the incidence of high-frequency hearing loss between the observation group and the control group in each level group (P>0.05) . The correlation between CNE after kurtosis adjustment and the incidence of high-frequency hearing loss was better than that before adjustment (after R(2) adjustment >before R(2) adjustment) . Conclusion: The effect of non-steady state noise on high-frequency hearing loss of workers in general equipment industry increases with the increase of CNE, and the effect of non-steady state noise on high-frequency hearing loss of workers is greater than that of steady noise. CNE' can be used to evaluate the hearing loss caused by non-steady state noise.


Assuntos
Perda Auditiva Provocada por Ruído , Ruído Ocupacional , Exposição Ocupacional , Perda Auditiva de Alta Frequência , Perda Auditiva Provocada por Ruído/epidemiologia , Humanos , Indústria Manufatureira , Ruído Ocupacional/efeitos adversos
2.
Artigo em Chinês | MEDLINE | ID: mdl-34365757

RESUMO

Objective: To analyse the dose-response relationships between the kurtosis metric of noise and noise-induced hearing loss (NIHL) and study the role of kurtosis in the evaluation of NIHL associated with non-Gaussian noise. Methods: From January 2012 to December 2017, a total of 1869 workers in seven manufacturing industries were selected as the study subjects. The basic data of the workers were investigated by questionnaire, personal noise waveform was collected for a long time, and pure tone hearing threshold was tested. The 8-hour continuous equivalent A sound level (L(Aeq, 8 h)) , cumulative noise exposure (CNE) and kurtosis structure indexes were calculated. The dose-response relationships between kurtosis and NIHL were analyzed by stratification analysis method, which controlled the influence of CNE, L(Aeq, 8 h), exposure duration, age and sex on hearing loss using high-frequency noise-induced permanent threshold shift (NIPTS(346)) and high-frequency noise-induced hearing loss (HFNIHL) as outcome indicators. Results: When CNE was <90 dB (A) ·year and ≥100 dB (A) ·year, NIPTS(346) in the extremely high kurtosis group was significantly greater than that in the Gaussian kurtosis, low kurtosis and medium kurtosis group (P<0.05) . In the workers exposed to L(Aeq, 8 h)<85 dB (A) and ≥94 dB (A) , NIPTS(346) in the extremely high kurtosis group was significantly greater than that in the Gaussian kurtosis group (P<0.05) . Among workers under the age of 50 or male workers, NIPTS(346) in the extremely high kurtosis group was significantly greater than that in the Gaussian kurtosis, low kurtosis and medium kurtosis group (P<0.05) . Kurtosis was positively correlated with NIPTS(346) (r=0.121, P<0.05) . When CNE was <100 dB (A) ·year, the detection rate of HFNIHL increased with the increase of kurtosis level (P<0.01) . Logistic regression analysis showed that kurtosis was an important influencing factor for HFNIHL (OR=1.321) . Conclusion: Kurtosis has a dose-response relationship with the detection rate of HFNIHL in noise exposed workers, and noise kurtosis is an influencing factor of NIHL.


Assuntos
Perda Auditiva Provocada por Ruído , Ruído Ocupacional , Exposição Ocupacional , Audição , Perda Auditiva Provocada por Ruído/etiologia , Humanos , Masculino , Indústria Manufatureira , Ruído Ocupacional/efeitos adversos , Exposição Ocupacional/efeitos adversos , Inquéritos e Questionários
3.
Artigo em Chinês | MEDLINE | ID: mdl-34365760

RESUMO

Objective: To investigate the occupational noise exposure characteristics and distribution of workers in wood furniture manufacturing industry and textile industry, so as to provide the basis for the establishment of noise source database and the prevention and control of noise hazards in China. Methods: In March 2020, a total of 653 workers in wood furniture manufacturing industry and textile industry were selected by cluster sampling method. A general questionnaire survey was conducted to measure the characteristics of noise exposure and analyze the changes of 8 h equivalent A-level (L(Aeq, 8 h)) and kurtosis of different industries and types of work. Results: The noise exposure L(Aeq, 8 h) of workers was (91.2±6.9) dB (A) , the median kurtosis and mean kurtosis were 37.0 and 105.0, respectively. 84.1% (549/653) of the workers had L(Aeq, 8 h)≥85 dB (A) , 49.0% (320/653) and 68.5% (447/653) of the workers had median kurtosis and mean kurtosis >10. The L(Aeq, 8 h) level of the textile industry was higher than that of the wooden furniture manufacturing industry, while the median and mean of kurtosis levels of the textile industry was lower than the wooden furniture manufacturing industry (P<0.01) . The L(Aeq, 8 h) levels of spinners and weavers were higher, and the median and mean of kurtosis of nailers were higher (P<0.05) . The mean and median of kurtosis were highly correlated among workers of two industries (r=0.80, P<0.01) . The L(Aeq, 8 h) was positively correlated with mean and median of kurtosis among workers of wooden furniture manufacturing industry (r=0.33 and 0.35, P<0.01) , while it was negatively correlated with mean and median of kurtosis among workers of textile industry (r=-0.45 and -0.40, P<0.01) . Conclusion: Indicators for noise exposure characteristics, such as L(Aeq, 8 h) and kurtosis vary greatly by industries and type of works. Multi-indicator joint measurement is conducive to comprehensive assessment of noise exposure and its health effects.


Assuntos
Ruído Ocupacional , Exposição Ocupacional , Humanos , Decoração de Interiores e Mobiliário , Indústria Manufatureira , Indústria Têxtil
4.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi ; 38(10): 721-725, 2020 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-33142371

RESUMO

Objective: To study the effect of p38 mitogen-activated protein kinase (MAPK) gene silencing on expression of apoptotic genes and oncogenes in hepatocytes treated with PM(2.5). Methods: From June to September 2019, according to the p38MAPK gene mRNA sequence provided by GenBank, three interfering sequences were designed and synthesized, ligated into PLVX-shRNA2-puro after annealing, and the recombinant lentiviral vector was transfected into L02 hepatocytes. The p38MAPK silencing cells were identified by real-time fluorescent quantitative PCR and western blotting. The normal L02 cells and p38MAPK silencing cells were treated with 50 µg/mL PM(2.5) water soluble solution, 10 µmol/L positive control Cr(6+), and a blank control group was set up, the treatment time was 24 h. The mRNA levels of oncogenes (c-fos, c-myc, k-ras) , tumor suppressor gene (p53) and apoptotic genes (Caspase-3, Caspase-8, Caspase-9) were detected by real-time PCR. The protein levels of oncogenes and apoptotic genes were detected by Western blotting. Results: The expression levels of p38MAPK mRNA and protein in p38MAPK gene silencing cells were significantly lower than those in L02 hepatocytes (P<0.05) , and the p38MAPK gene silencing cell line was successfully constructed. Compared with the blank control group, the expression levels of the oncogenes c-fos, c-myc, k-ras and the apoptosis genes Caspase-3, Caspase-8 and Caspase-9 increased, the expression level of tumor suppressor gene p53 decreased in the L02 hepatocyte group treated with PM(2.5) water soluble matter, and the differences were statistically significant (P<0.05) . Compared with the L02 hepatocytes group treated with PM(2.5) water soluble matter, the expression levels of the oncogenes c-fos, c-myc, k-ras and apoptosis genes Caspase-3, Caspase-8 and Caspase-9 decreased, the expression level of tumor suppressor gene p53 increased in the p38MAPK gene silencing cells group treated with PM(2.5) water soluble matter, and the differences were statistically significant (P<0.05) . Conclusion: PM(2.5) has effects on the expression of oncogenes, tumor suppressor genes and apoptotic genes in L02 hepatocytes, while p38MAPK gene silencing can inhibit the effects of PM(2.5) on L02 hepatocytes.


Assuntos
Hepatócitos , Oncogenes , Apoptose , Inativação Gênica , Humanos , Material Particulado
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(5): 738-742, 2020 May 10.
Artigo em Chinês | MEDLINE | ID: mdl-32447917

RESUMO

Objective: To explore the daily dietary behaviors of girls under precocious puberty and provide targeted measures for the prevention of precocious puberty. Methods: A case control study was conducted in a hospital in Shenzhen between September 2016 and December 2018. Girls with diagnosed precocious puberty were selected as case group. A 1∶1 matching was conducted. The control group was from 26 primary schools in Shenzhen. Dietary survey was conducted in parents, completing a self-administered questionnaire. Frequencies of 12 kinds of food intakes were investigated and dietary patterns were analyzed. Conditional logistic regression was used to analyze the relationship between dietary patterns and precocious puberty. Results: A total of 568 girls were included in the study. Among them, those aged 8-year-old accounted for highest proportion (43.8%). The median of age was same in both case group and control group (8 years-old). There was no significant difference in ethnic group between two groups (P>0.05). The medians of height, weight and BMI of case group were 135.0 cm, 30.2 kg and 16.6 kg/m(2), respectively, which were all higher than those of the control group (129.2 cm, 25.0 kg and 15.3 kg/m(2)), the differences were significant (P<0.05). There was significant difference in nutritional assessment result between two groups (P< 0.05). Three dietary patterns were defined, i.e. balanced pattern, high calorie and fat pattern and high protein diet pattern. The cumulative rate of variance contribution of the three dietary patterns was 0.541 2. The differences in the prevalence of three dietary patterns between two groups were significant (χ(2)=4.41, χ(2)=49.24, χ(2)=39.68, P<0.05 respectively). Data from the multivariate regression analysis showed that both balanced dietary pattern (OR=0.633, 95%CI: 0.504-0.769) and high protein diet pattern (OR=0.622, 95%CI: 0.498-0.776) were protective factors for precocious puberty, while high calorie and fat pattern was risk factors (OR=1.850, 95%CI: 1.461-2.342). Conclusions: Balanced dietary pattern was common in school-aged girls. High calorie and fat pattern was risk factor for precocious puberty. Children should be encouraged to develop a balanced dietary habit and increasing the intake of legumes and fish since they are beneficial to normal growth and development.


Assuntos
Puberdade Precoce , Estudos de Casos e Controles , Criança , Dieta , Comportamento Alimentar , Feminino , Humanos , Instituições Acadêmicas
6.
Zhonghua Yi Xue Za Zhi ; 97(7): 522-524, 2017 Feb 21.
Artigo em Chinês | MEDLINE | ID: mdl-28260292

RESUMO

Objective: To investigate the characteristics of superior petrosal vein (SPV) and its influence on the surgical field in microvascular decompression (MVD) for trigeminal neuralgia (TN), and to analyze the effect of the surgical treatment of SPV on the surgical approach, indication and prognosis. Methods: The clinical data of 280 patients with trigeminal neuralgia between Jan. 2013 and Jun. 2016 were collected, including the trunks and the branches of SPV, intraoperative electrocoagulation status, the surgery outcome and complications. Results: The petrosal vein during the operation was fully preserved in 152 cases (54.29%). The SPV were completely sectioned in 25 cases (8.92%), while some branches of SPV were sectioned in 103 cases (36.79%). We found that SPV have 1 to 3 trunks, accounted for 67 cases (23.90%), 168 cases (60%), and 45 cases (16.10%), while the SPV with 1 to 4 branches accounted for 17 cases (6.07%), 112 cases (40%), 136 cases (48.57%), and 15 cases (5.36%). The SPV was identified as offending vessel in 17 cases (6.07%). One patient with cutoff SPV trunk encountered cerebellar infarction and recovered completely at 2 weeks after MVD by using intravenous medication. Conclusions: MVD is the recommended treatment method for PTN, mostly SPV is unnecessary to be sectioned completely and small branches of SPV could be sacrificed. Very few patients may develop cerebellar infarction or hematoma.


Assuntos
Veias Cerebrais , Cirurgia de Descompressão Microvascular , Neuralgia do Trigêmeo , Eletrocoagulação , Humanos , Nervo Trigêmeo
8.
Cell Mol Biol (Noisy-le-grand) ; 61(8): 31-6, 2015 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-26667770

RESUMO

Genotoxic stress activates checkpoint signaling pathways that activate the checkpoint kinases ATM and ATR, halt cell cycle progression, and promote DNA repair. A number of proteins act in concert with ATR to phosphorylate Chk1, including RAD17, the RAD9-RAD1-HUS1 complex, ATR/ATRIP and TopBp1. However, how these proteins involved act in concert with one another to propagate and maintain the checkpoint response is not well understood. Here, we reported that upregulation of RAD9 protein increased the quantity of ATRIP, suggesting that RAD9 activation will induce more efficient accumulation of ATRIP in vivo. Furthermore, the DNA damage-induced ATRIP foci formation was faster in the mRad9-/- ES cells. Also, ATRIP interacts specifically with RAD9, but not HUS1 and RAD1. Taken together, we suggested that RAD9 could affect both the ATRIP protein levels and DNA damage-induced ATRIP foci formation. Thus, we propose a role of RAD9 in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas de Ciclo Celular/genética , Reparo do DNA , Proteínas de Ligação a DNA/genética , DNA/genética , Proteínas Quinases/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Proteínas Mutadas de Ataxia Telangiectasia/genética , Proteínas Mutadas de Ataxia Telangiectasia/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Proteínas de Ciclo Celular/metabolismo , Quinase 1 do Ponto de Checagem , Cromatina/química , Cromatina/efeitos dos fármacos , Cromatina/metabolismo , DNA/metabolismo , Dano ao DNA , Proteínas de Ligação a DNA/metabolismo , Exonucleases/genética , Exonucleases/metabolismo , Regulação da Expressão Gênica , Células HEK293 , Humanos , Hidroxiureia/farmacologia , Camundongos , Células-Tronco Embrionárias Murinas/citologia , Células-Tronco Embrionárias Murinas/efeitos dos fármacos , Células-Tronco Embrionárias Murinas/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Fosforilação , Proteínas Quinases/metabolismo , Transdução de Sinais , Especificidade da Espécie
9.
J Pediatr Surg ; 34(11): 1725-7, 1999 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-10591580

RESUMO

BACKGROUND: Urologic complications after pediatric renal transplantation can adversely effect the outcome and may result in decreased graft survival. Efforts to prevent these complications are worthwhile. This study investigates the incidence of these complications in a clinical transplant program and reports on an animal model used to investigate one possible cause. METHODS: In the clinical study, the results of a pediatric renal transplant program at a large children's hospital for a 5(1/2)-year period were reviewed with special attention paid to patients suffering ureteral necrosis. In the experimental study, 9 swine underwent laparotomy, bilateral complete infrahilar ureteric dissection, and extravesical ureteroneocystostomy. On the left side only, the renal and adrenal veins were ligated. The arterial supply remained intact. The right side did not undergo vessel ligation and served as the control. Three pigs each were killed at 3, 8, and 15 days. Kidneys, ureters and a cuff of bladder were examined histologically. RESULTS: In the clinical study 75 renal transplants were performed with a total of 5 cases of early ureteral necrosis. Two of these 5 displayed venous congestion and ischemia, and 2 were associated with kidneys displaying primary nonfunction of the graft. Seventy-one of 75 grafts are continuing to function. One of the 4 early graft losses also had an ischemic ureter. In the experimental study all right kidneys and ureters were normal. All left kidneys had complete hemorrhagic necrosis. Necrosis also was found in 5 of 9 proximal left ureters and in 7 of 9 distal left ureters. Viable left ureters displayed moderate to severe submucosal and periureteric hemorrhage. Four of 9 ureters displayed more damage distally than proximally. The extent of necrosis was similar at 3, 8, and 15 days. CONCLUSION: In both clinical and experimental studies, venous congestion and subsequent ischemia have been shown to be important causes of ureteral necrosis after renal transplantation.


Assuntos
Isquemia/complicações , Transplante de Rim/efeitos adversos , Rim/irrigação sanguínea , Ureter/irrigação sanguínea , Doenças Ureterais/etiologia , Doenças Ureterais/patologia , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Modelos Animais de Doenças , Estudos de Avaliação como Assunto , Feminino , Rejeição de Enxerto , Sobrevivência de Enxerto , Humanos , Rim/patologia , Transplante de Rim/métodos , Masculino , Necrose , Prognóstico , Índice de Gravidade de Doença , Suínos , Ureter/patologia , Veias
10.
J Pediatr Surg ; 34(5): 825-7; discussion 828, 1999 05.
Artigo em Inglês | MEDLINE | ID: mdl-10359188

RESUMO

BACKGROUND/PURPOSE: Spinal dysraphism and neurovesical dysfunction (NVD) frequently are associated in children with anorectal malformations (ARM). This study compares the urodynamic data from a selected group of patients with the results of their spinal and urologic imaging studies. METHODS: Twenty-six children (20 with isolated imperforate anus and six with persistent cloacal malformations) were investigated. All patients were evaluated with leak point pressures (LPP), renal ultrasound scan, and voiding cystourethrography. Eight children had urodynamics performed before and after posterior sagittal anorectoplasty (PSARP). The spinal cord was assessed using ultrasonography or magnetic resonance imaging. Current urologic status was obtained to provide long-term follow-up. RESULTS: Twenty-one of 26 children demonstrated elevated LPPs above the established normal value of 40 cm H2O, and 15 of these children had normal spinal imaging study findings. Uroradiographic studies findings showed that 12 of 21 children with elevated LPPs had hydronephrosis or vesicoureteral reflux with seven of these patients having normal spinal cords. LPPs in the eight patients with pre- and postoperative studies were 74 +/- 14.7 cm H2O and 68 +/- 31.8 cm H2O (mean +/- SD), respectively. CONCLUSIONS: These urodynamic and radiographic data confirm that NVD (elevated LPP) is common in patients with anorectal malformations despite normal spinal cords. Bladder dysfunction does not appear to be a sequelae of a properly performed PSARP. Patients with ARM and any uroradiographic or clinical urologic abnormality should undergo urodynamic testing even though the spinal studies are normal.


Assuntos
Anus Imperfurado/fisiopatologia , Cloaca/anormalidades , Reto/anormalidades , Bexiga Urinaria Neurogênica/fisiopatologia , Anus Imperfurado/complicações , Criança , Pré-Escolar , Humanos , Lactente , Estudos Prospectivos , Reto/cirurgia , Bexiga Urinaria Neurogênica/complicações , Urodinâmica
11.
J Pediatr Surg ; 32(1): 84-7, 1997 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9021577

RESUMO

Unilateral testicular trauma in the postpubertal male can lead to alterations in semen analysis, but it is not clear what effect this has on fertility. To better understand how surgical treatment of testicular trauma affects both fertility and testicular histology the following study was performed. Eighty postpubertal Lewis rats were divided into eight equal groups with one group serving as a control. In the 70 remaining rats the left testicle was subjected to blunt or penetrating injury. The testicles were either left untreated, were removed, or were repaired with sutures or mesh before being returned to the scrotum. Following recovery, each male was allowed to mate to determine fertility. Fertility rates were significantly lower in all postinjury groups except the postinjury orchiectomy group. Histological analyses showed nonspecific inflammation, smaller tubules, and impaired spermatogenesis in all postinjury testicles regardless of the type of treatment. Contralateral testicles had no evidence of autoimmune injury and were essentially identical to the control group. In the postpubertal Lewis rat, unilateral testicular trauma leads to impaired fertility unless the injured testicle is removed soon after the injury. Various methods of repairing the injury did not improve fertility. In spite of the impaired fertility, the contralateral testicle appears histologically normal.


Assuntos
Fertilidade , Testículo/lesões , Ferimentos não Penetrantes/cirurgia , Ferimentos Penetrantes/cirurgia , Animais , Doenças Autoimunes/patologia , Células Intersticiais do Testículo/patologia , Masculino , Orquiectomia , Orquite/patologia , Tamanho do Órgão , Ratos , Ratos Endogâmicos Lew , Escroto/cirurgia , Sêmen/química , Túbulos Seminíferos/patologia , Células de Sertoli/patologia , Espermatogênese , Telas Cirúrgicas , Técnicas de Sutura , Testículo/patologia , Testículo/fisiopatologia , Testículo/cirurgia , Ferimentos não Penetrantes/patologia , Ferimentos não Penetrantes/fisiopatologia , Ferimentos Penetrantes/patologia , Ferimentos Penetrantes/fisiopatologia
12.
Wei Sheng Yan Jiu ; 26(1): 2-5, 1997 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-15747450

RESUMO

In order to evaluate the roles of sex and age in the formation of lung tissue DNA adducts, the levels of DNA adducts in human lung tissues from both patients with or without lung cancer were examined. The results showed there was a low correlation coefficient (r=0.11) between age and DNA adducts, but a significant difference in sex being observed. Smoking is a strong factor in the formation of DNA adducts. However, the role of endocrine can not be ruled out.


Assuntos
Adutos de DNA/genética , Dano ao DNA/genética , DNA de Neoplasias/genética , Neoplasias Pulmonares/genética , Pulmão/metabolismo , Fumar/efeitos adversos , Fatores Etários , Idoso , Adutos de DNA/isolamento & purificação , DNA de Neoplasias/isolamento & purificação , Humanos , Neoplasias Pulmonares/etiologia , Fatores Sexuais
13.
J Pediatr Surg ; 31(2): 225-8, 1996 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-8938346

RESUMO

As an alternative to bladder mucosa, free grafts of tubularized peritoneum were used as urethral substitutes in a rabbit model of hypospadias. In group 1, six mature rabbits underwent partial penile urethrectomy followed by interposition of a 2-cm-long peritoneal-lined tube graft. These animals had urethrograms performed at 3 months and were killed at 6 (n = 4) or 12 (n = 2) months. In group 2, six rabbits underwent total penile urethrectomy with placement of 3-cm-long grafts. These animals were killed 1 to 4 weeks after surgery. Clinical assessment and gross examination of the 12 rabbits showed no urinary retention, two small fistulas at the proximal anastomosis, and no strictures or diverticular. At 6 and 12 months (group 1) the urethra had healed completely and the graft edges were not visible. In group 2, 1 to 4 weeks after surgery the graft was intact and the interface between the graft and native urethra was visible. Histological studies of the grafts were compared with control peritoneum. At 1 week, a high-density single-cell layer was present. Beginning at 2 weeks, a multilayered epithelium was present, which became more organized in the older grafts. Neovascularity became visible in the subepithelial layer at 2 weeks. Acute inflammatory cells were present early and were replaced by a palisading layer of lymphocytes and plasma cells in the older grafts. Minimal fibrosis was observed. Tubularized peritoneal free grafts are a promising new urethral substitute. Graft placement is technically simple. Replacement of the peritoneum by a multilayered epithelium resembling transitional epithelium occurs early after graft placement. As much as 1 year later, minimal scarring is present.


Assuntos
Hipospadia/cirurgia , Peritônio/transplante , Uretra/cirurgia , Animais , Modelos Animais de Doenças , Epitélio/fisiologia , Masculino , Peritônio/fisiologia , Coelhos
14.
J Urol ; 146(2 ( Pt 2)): 544-7, 1991 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-1907329

RESUMO

The long-term effects of extracorporeal shock wave lithotripsy (ESWL*) on children treated for renal calculi are unclear. To study the long-term bio-effects of this mode of treatment on the immature animal we evaluated 30 New Zealand white rabbits at 7 weeks of age for weight, serum blood urea nitrogen and creatinine, and arterial blood pressure after which they underwent left nephrectomy. Each group of 5 rabbits received ESWL of varying levels (500 to 3,000 shock waves) to the remaining right kidney using the Northgate SD3 lithotriptor (spark gap mediated). One control group received no shock waves. At maturity (16 weeks) the aforementioned parameters were measured again, and the kidneys and any grossly abnormal adjacent organs were examined. We found no significant change in total animal growth, renal growth, renal function or perirenal organs in the post-ESWL groups versus the control group. All post-ESWL groups had an increase in mean arterial blood pressure versus the control group with 3 of 6 groups showing significant increases (p less than 0.05). Histological renal changes, seen at all energy levels of ESWL delivered, included interstitial fibrosis, tubular atrophy, glomeruli destruction, capsular thickening, perivascular fibrosis and mild arteriole wall thickening. Changes were proportional to the number of shocks received. We conclude that ESWL delivered to immature animals does not significantly affect renal growth and function but it can cause significant permanent histological renal changes even at low doses and may result in an increase in adult mean arterial blood pressure.


Assuntos
Rim/patologia , Litotripsia/efeitos adversos , Animais , Fibrose/etiologia , Hipertensão/etiologia , Rim/crescimento & desenvolvimento , Rim/fisiologia , Nefropatias/etiologia , Hepatopatias/etiologia , Assistência de Longa Duração , Coelhos , Aderências Teciduais/etiologia
15.
J Urol ; 144(4): 1041-3, 1990 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-2398552

RESUMO

A new technique for nonrefluxing ureteral replacement using ileum has been developed. Five adult mongrel dogs were operated using this nonrefluxing ileal ureteral technique. Subsequent reoperation showed in every case the preservation of renal function, a nonrefluxing system and no evidence of pyelonephritis or hydronephrosis. Based on the intussuscepted ileal nipple used in the Kock continent ileostomy, this technique has application in candidates for urinary undiversion, situations of ureteral loss due to trauma and fibrosis, and in patients with tuberculous strictured ureters.


Assuntos
Íleo/cirurgia , Ureter/cirurgia , Derivação Urinária/métodos , Animais , Cães , Reoperação
16.
J Urol ; 143(2): 377-80, 1990 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2299738

RESUMO

A juvenile animal model has been developed to study the growth potential of ileum in the urinary tract. Patch ileocystoplasties of known surface area were constructed in dogs of Group 1. Nonrefluxing ileal nipple valves of known length were created to replace one ureter in dogs of Group 2. After the juvenile animals grew and at minimum doubled their weight, they were reoperated and augmentation surface areas were remeasured at various physiologic intravesical pressures. Nipple valve lengths were remeasured after cystograms ruled out reflux. Results show that the bowel augmentation and an identical control segment increased in surface area proportionate to animal growth and that hydrostatic dilation caused further surface area increase. Nipple valves did not grow and in fact shortened, but remained nonrefluxing. The conclusion is that in the pediatric population, consideration should be given to downscaling the size of bladder augmentations or diversions in anticipation of future bowel growth, but that one should not shorten the ileal nipple valves.


Assuntos
Íleo/transplante , Transplante Heterotópico , Animais , Cães , Feminino , Íleo/crescimento & desenvolvimento , Bexiga Urinária/cirurgia , Derivação Urinária , Sistema Urinário
17.
Steroids ; 46(4-5): 867-81, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-3837956

RESUMO

In an attempt to confirm where in the testosterone (T) biosynthetic pathway of the rat testis ketoconazole (KTZ) inhibits T production, rat testicular mince was incubated with either 10 micrograms/ml or 100 micrograms/ml KTZ in the presence and absence of hCG (1 IU), and intratesticular pregnenolone (delta 5P), progesterone (P), 17-alpha-hydroxyprogesterone (17 alpha-HP), androstenedione (A) and testosterone (T) were assayed. In the absence of hCG, 10 micrograms/ml KTZ was sufficient to reduce intratesticular T by 80%. At this concentration of KTZ, intratesticular 17 alpha-HP (ng/g testis, mean +/- SEM) increased from 0.3 +/- 0.1 to 1.3 +/- 0.2 (p less than 0.0025), whereas intratesticular A decreased from 84 +/- 7 to 17 +/- 1 (p less than 0.005). KTZ did not inhibit the conversion of P to 17 alpha-HP. From these data it was concluded that KTZ has its inhibitory effect on testosterone biosynthesis in the rat testis primarily at the step catalyzed by the 17,20 desmolase enzyme.


Assuntos
Cetoconazol/farmacologia , Testículo/efeitos dos fármacos , Testosterona/biossíntese , 17-alfa-Hidroxiprogesterona , Androstenodiona/biossíntese , Animais , Gonadotropina Coriônica/farmacologia , Hidroxiprogesteronas/biossíntese , Masculino , Pregnenolona/biossíntese , Progesterona/biossíntese , Ratos , Testículo/metabolismo
18.
Fertil Steril ; 44(1): 125-32, 1985 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2861116

RESUMO

In an attempt to determine whether the production and synthesis of testosterone (T) by the testis is impaired by the cryptorchid state, the ability of the cryptorchid rat testis to form T was assessed at various time periods into adulthood after the surgical induction of cryptorchidism in the newborn period. The intratesticular T content of the descended testis rose from 0.3 ng/testis at 14 days of age to 71.2 ng/testis at adulthood (day 56); whereas in the cryptorchid testis, the values were 0.4 ng/testis and 2.0 ng/testis, respectively, at the same ages (P less than 0.001). For determination of the site of inhibition of T biosynthesis in the cryptorchid testis, the enzymatic activities (17 alpha-hydroxylase, 17,20-desmolase, and 17 beta-hydroxysteroid dehydrogenase) in the delta 4T biosynthetic pathway were measured. All these enzyme activities in the cryptorchid testis were inhibited at 56 days of age by about 80% when compared with the descended testis (P less than 0.01). These data suggest that cryptorchidism exerts a deleterious effect on the ability of the Leydig cells to synthesize T and may in part explain the abnormal morphology and resultant infertility seen in patients with cryptorchidism.


Assuntos
Hidrocarboneto de Aril Hidroxilases , Criptorquidismo/metabolismo , Testículo/metabolismo , Testosterona/biossíntese , 17-Hidroxiesteroide Desidrogenases/metabolismo , Aldeído Liases/metabolismo , Animais , Criptorquidismo/enzimologia , Masculino , Tamanho do Órgão , Ratos , Ratos Endogâmicos , Maturidade Sexual , Esteroide 17-alfa-Hidroxilase , Esteroide Hidroxilases/metabolismo , Testículo/enzimologia , Testosterona/sangue
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