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1.
Med Sci Monit ; 26: e921058, 2020 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-32279065

RESUMO

BACKGROUND This study analyzed the distribution of Rh serological phenotype in people living in Hangzhou, China, and assessed the necessity of its routine clinical detection and homotypic infusion. MATERIAL AND METHODS Blood donors and patients who might need blood transfusion were enrolled into the study, and ABO and 5 major Rh serological antigens (C, c, D, E, and e) were routinely detected. The consistent ABO and Rh serological phenotype blood was transfused between the blood donors and recipients. Irregular antibodies were screened and identified in patients before the blood transfusion. Then, the transfusion adverse effects were monitored and compared with the previous data in the hospital. RESULTS The phenotypic frequencies of Rh blood groups were D>C>E>c>e. The CCDee was the most common phenotype and CcdEe was the least common. The detection rate of unexpected antibodies gradually increased, while the unexpected antibodies slowly decreased in the Rh system. There was a correlation between the isotypic infusion of 5 Rh antigens and the detection rate of antibodies in the Rh system (R=0.845). The adverse effects of blood transfusion declined from 19.95% in 2011 with just homotypic ABO infusion to 3.098% in 2019 with the transfusion of homotypic ABO and the 5 major Rh serological antigens. CONCLUSIONS The consistency of the transfusion with ABO and 5 significant Rh serological antigens could prevent and decrease the high frequency production of isoantibodies, which is of vital importance in reducing the incidence rate of adverse effects in patients receiving transfusions.


Assuntos
Tipagem e Reações Cruzadas Sanguíneas , Transfusão de Sangue , Sistema do Grupo Sanguíneo Rh-Hr/sangue , Reação Transfusional , Sistema ABO de Grupos Sanguíneos/sangue , Doadores de Sangue , China , Feminino , Humanos , Masculino , Fenótipo
2.
PLoS Comput Biol ; 15(10): e1006667, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31609973

RESUMO

A fundamental and recurrent question in systems neuroscience is that of assessing what variables are encoded by a given population of neurons. Such assessments are often challenging because neurons in one brain area may encode multiple variables, and because neuronal representations might be categorical or non-categorical. These issues are particularly pertinent to the representation of decision variables in the orbitofrontal cortex (OFC)-an area implicated in economic choices. Here we present a new algorithm to assess whether a neuronal representation is categorical or non-categorical, and to identify the encoded variables if the representation is indeed categorical. The algorithm is based on two clustering procedures, one variable-independent and the other variable-based. The two partitions are then compared through adjusted mutual information. The present algorithm overcomes limitations of previous approaches and is widely applicable. We tested the algorithm on synthetic data and then used it to examine neuronal data recorded in the primate OFC during economic decisions. Confirming previous assessments, we found the neuronal representation in OFC to be categorical in nature. We also found that neurons in this area encode the value of individual offers, the binary choice outcome and the chosen value. In other words, during economic choice, neurons in the primate OFC encode decision variables in a categorical way.


Assuntos
Comportamento de Escolha/fisiologia , Biologia Computacional/métodos , Tomada de Decisões/fisiologia , Algoritmos , Animais , Análise por Conglomerados , Lobo Frontal/fisiologia , Macaca mulatta , Modelos Teóricos , Neurônios/fisiologia , Córtex Pré-Frontal/fisiologia , Recompensa
3.
J Immunol Res ; 2019: 6804806, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30944836

RESUMO

Background: OX40, which is also known as tumor necrosis factor receptor superfamily member 4 (TNFRSF4), and its ligand (OX40L) play a critical role in the pathogenesis of autoimmune diseases. Immune thrombocytopenia (ITP), a hemorrhagic autoimmune disorder, is characterized by low platelet counts that are predominantly caused by antiplatelet autoantibodies. In this study, we firstly investigated the clinical significance of OX40 and OX40L expression in the pathogenesis of ITP in patients. Methods: Fifty-four newly diagnosed ITP patients and 24 healthy controls (HCs) were enrolled in this study. The percentage of OX40+CD4+T cells among CD4+T cells was analyzed by flow cytometry, and the expression levels of OX40 and OX40L mRNA were analyzed by quantitative real-time PCR. Plasma soluble OX40L (sOX40L) levels were analyzed by ELISA, and plasma levels of antiplatelet autoantibodies were analyzed by a solid-phase technique. Results: Compared with HCs, the frequencies of OX40+CD4+T cells were significantly increased in ITP patients, particularly in patients with positive antiplatelet autoantibodies compared to those with negative antiplatelet autoantibodies. The elevated frequencies of OX40+CD4+T cells were negatively correlated with low platelet counts in patients with positive antiplatelet autoantibodies. Plasma sOX40L levels in ITP patients were significantly greater than those in HCs and increased in patients with positive antiplatelet autoantibodies compared to those with negative antiplatelet autoantibodies. Plasma sOX40L levels were negatively correlated with low platelet counts in patients with positive antiplatelet autoantibodies. Additionally, the mRNA expression levels of OX40 and OX40L in PBMCs from ITP patients were also notably greater than those from HCs, and the expression levels of OX40 and OX40L were significantly different in ITP patients with positive and negative antiplatelet autoantibodies. Conclusion: These data indicated that increased expression levels of OX40 and OX40L were involved in the pathogenesis of ITP, and OX40 and OX40L may be valuable therapeutic targets for ITP.


Assuntos
Ligante OX40/genética , Púrpura Trombocitopênica Idiopática/imunologia , Receptores OX40/genética , Adulto , Idoso , Autoanticorpos/sangue , Plaquetas/imunologia , Linfócitos T CD4-Positivos/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Citometria de Fluxo , Humanos , Leucócitos Mononucleares , Masculino , Pessoa de Meia-Idade , Ligante OX40/sangue , Púrpura Trombocitopênica Idiopática/patologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores OX40/imunologia , Adulto Jovem
5.
Semin Reprod Med ; 36(1): 66-72, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-30189453

RESUMO

Despite polycystic ovary syndrome (PCOS) being the most common endocrine condition affecting reproductive-aged women, studies have shown the information needs of PCOS consumers are not currently met. The expressed need by women with PCOS for accessible, evidence-based personalized PCOS information informed the design and development of the PCOS mobile tool-AskPCOS. The App provides a range of unique features such as: evidence-based PCOS health information, self-diagnostic function, a question prompt list to optimize health practitioner engagement, and a commonly asked questions list. A five-phase App development process involved extensive stakeholder consultation, system architecture design, development of the content repository, system prototyping, and evaluation. AskPCOS is the first evidence-based, consumer-driven mobile App developed by women and for women with PCOS and utilizes innovative technology to empower PCOS consumers and optimize health outcomes. The App content repository is enhanced by the best available evidence and expertise extracted from the International Evidence-Based Guideline in PCOS (2018). The AskPCOS App has extensive data capture capability through the usage of data gathering functions and backend analytics to optimize responsiveness to consumer needs. In addition, the multilingual content and not-for-profit cost model is designed to facilitate worldwide adoption of the tool, and address PCOS information inequities in developing countries.


Assuntos
Letramento em Saúde , Aplicativos Móveis , Síndrome do Ovário Policístico , Telemedicina , Feminino , Humanos
6.
J Immunol Res ; 2018: 8972572, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30956992

RESUMO

Background: Follicular helper T (Tfh) cells are critical for high-affinity antibody generation and B cell maturation and differentiation, which play important roles in autoimmune diseases. Graves' disease (GD) is one prototype of common organ-specific autoimmune thyroid diseases (AITD) characterized by autoreactive antibodies, suggesting a possible role for Tfh cells in the pathogenesis of GD. Our objective was to explore the role of circulating Tfh cell subsets and associated plasma cells (PCs) in patients with GD. Methods: Thirty-six patients with GD and 20 healthy controls (HC) were enrolled in this study. The frequencies of circulating Tfh cell subsets and PCs were determined by flow cytometry, and plasma cytokines, including interleukin- (IL-) 21, IL-4, IL-17A, and interferon- (IFN-) γ, were measured using an enzyme-linked immunosorbent assay (ELISA). The mRNA expression of transcription factors (Bcl-6, T-bet, GATA-3, and RORγt) in peripheral blood mononuclear cells (PBMCs) was evaluated by real-time quantitative PCR. Results. Compared with HC, the frequencies of circulating CD4+CXCR5+CD45RA-Tfh (cTfh) cells with ICOS and PD-1 expression, the Tfh2 subset (CXCR3-CCR6-Tfh) cells, and PCs (CD19+CD27highCD38high) were significantly increased in the GD patients, but the frequencies of Tfh1 (CXCR3+CCR6-Tfh) and Tfh17 (CXCR3-CCR6+Tfh) subset cells among CD4+T cells were significantly decreased in GD patients. The plasma concentrations of IL-21, IL-4, and IL-17A were elevated in GD patients. Additionally, a positive correlation was found between the frequency of PD-1+Tfh cells (Tfh2 or PCs) and plasma IL-21 concentration (or serum TPO-Ab levels). The mRNA levels of transcription factors (GATA-3 and RORγt) were significantly increased, but T-bet and Bcl-6 mRNA expression was not obviously varied in PBMCs from GD patients. Interestingly, Tfh cell subsets and PCs from GD patients were partly normalized by treatment. Conclusion: Circulating Tfh cell subsets and PCs might play an important role in the pathogenesis of GD, which are potential clues for GD patients' interventions.


Assuntos
Doença de Graves/sangue , Doença de Graves/imunologia , Linfócitos T Auxiliares-Indutores/classificação , Linfócitos T Auxiliares-Indutores/imunologia , Adulto , Citocinas/sangue , Citocinas/imunologia , Feminino , Citometria de Fluxo , Humanos , Interleucinas/sangue , Masculino , Pessoa de Meia-Idade , Plasmócitos/imunologia
7.
BMJ Open ; 7(9): e014881, 2017 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-28864688

RESUMO

INTRODUCTION: The best methods for inducing analgesia and sedation for gastroscopy are still debated but finding an adequate regimen of sedation/analgesia is important. Stimulation of the larynx under sedation can cause reflex responses. Propofol with opioids has been recommended for gastroscopy sedation but the effects on cough reflex suppression remain unclear. This trial will evaluate the effects of propofol combined with small doses of dezocine, oxycodone, sufentanil or fentanyl for gastroscopy. We hypothesise that better performance may be obtained with a combination of propofol and oxycodone. We will observe the incidence and degree of reflex coughing and gagging under sedation when using propofol combined with one of the above drugs or propofol alone. METHODS AND ANALYSIS: This will be a prospective, randomised, double-blind, controlled trial. ASA I-II level patients aged 18-65 years and scheduled for gastroscopy will be included. It is planned that 500 subjects will be randomised to intravenously receive 2-2.2 mg/kg propofol plus 0.5-0.8 µg/kg fentanyl (fentanyl group), 2-2.2 mg/kg propofol plus 0.05-0.08 µg/kg sufentanil (sufentanil group), 2-2.2 mg/kg propofol plus 0.04-0.05 mg/kg dezocine (dezocine group), 2-2.2 mg/kg propofol plus 0.04-0.05 mg/kg oxycodone (oxycodone group), or 2.4-3 mg/kg propofol plus 2-2.5 mL saline (control group) for sedation. The primary endpoint is the incidence and degree of reflex coughing and gagging. The secondary endpoints include the occurrence of discomfort or side effects, the use of jaw thrust, assisted ventilation or additional propofol, recovery time, duration of procedure and Steward score. ETHICS AND DISSEMINATION: This study has been approved by the Institutional Ethics Committee for Clinical Research of Zhongda Hospital, Affiliated to Southeast University (No. 2015ZDSYLL033.0). The results of the trial will be published in an international peer-reviewed journal. TRIAL REGISTRATION: This study has been registered with the Chinese Clinical Trial Register (No. ChiCTR-ICR-15006952). TRIAL STATUS: At the time of manuscript submission, the study was in the recruitment phase.


Assuntos
Analgésicos Opioides/uso terapêutico , Sedação Consciente , Tosse/prevenção & controle , Gastroscopia/efeitos adversos , Hipnóticos e Sedativos/farmacologia , Propofol/uso terapêutico , Adolescente , Adulto , Idoso , Analgésicos Opioides/farmacologia , Antitussígenos/farmacologia , Antitussígenos/uso terapêutico , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Tosse/etiologia , Método Duplo-Cego , Quimioterapia Combinada , Feminino , Fentanila/farmacologia , Engasgo/prevenção & controle , Humanos , Masculino , Pessoa de Meia-Idade , Dor/etiologia , Dor/prevenção & controle , Propofol/farmacologia , Estudos Prospectivos , Reflexo/efeitos dos fármacos , Projetos de Pesquisa , Tetra-Hidronaftalenos/farmacologia , Adulto Jovem
8.
Clin Cancer Res ; 23(13): 3297-3306, 2017 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-28039267

RESUMO

Purpose: Patients with relapsed/refractory acute lymphocytic leukemia (R/R ALL) have a poor prognosis. Chimeric antigen receptor-modified T cells against CD19 (CART19) have displayed anti-leukemia activities. However, data from systemic trials in Chinese patients are limited.Experimental Design: T cells transduced with CD19-directed CAR lentiviral vectors were infused in patients with R/R ALL under fludarabine- and cyclophosphamide-based lymphodepletion. The postinfusion responses, toxicities, expansion, and persistence of CART19s in enrolled patients were observed and monitored.Results: We enrolled 15 patients with R/R ALL. The median transduction efficiency of CART19s was 33%. In vitro cytotoxicity assays were conducted and showed prominent antileukemia activities with CART19s. The patients received CART19s infusion at doses of 1.1 × 106/kg to 9.8 × 106/kg. Twelve patients achieved complete remission 1 month after CART19s infusion. CART19s expanded and persisted in peripheral blood and bone marrow. At 150 days, the overall survival rate and leukemia-free survival rate were 65.5% and 37.8%, respectively. The cumulative incidence of relapse and the nonrelapse mortality rate were 54.5% and 7.7%, respectively. Four patients underwent subsequent haploidentical hematopoietic stem cell transplantation. In this trial, 10 patients experienced cytokine release syndrome (CRS). Grade 3 CRS developed in 40% of patients and was associated with a higher disease burden on day -1 and a higher number of previous relapses.Conclusions: This trial demonstrated potent antileukemia activities of CART19s in Chinese patients with R/R ALL. Disease relapse remained the main obstacle. However, patients with a high risk of relapse after CART19s might benefit from subsequent haploidentical hematopoietic stem cell transplantation. Clin Cancer Res; 23(13); 3297-306. ©2016 AACR.


Assuntos
Antígenos CD19/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Receptores de Antígenos de Linfócitos T/imunologia , Linfócitos T/imunologia , Adulto , China , Intervalo Livre de Doença , Feminino , Doença Enxerto-Hospedeiro/tratamento farmacológico , Doença Enxerto-Hospedeiro/imunologia , Transplante de Células-Tronco Hematopoéticas/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Receptores de Antígenos de Linfócitos T/administração & dosagem , Recidiva , Indução de Remissão , Linfócitos T/efeitos dos fármacos
9.
Medicine (Baltimore) ; 95(41): e5160, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27741149

RESUMO

We aimed to evaluate whether blood conservation strategies including intraoperative autologous donation (IAD) could reduce perioperative blood transfusion for patients undergoing cardiac valve replacement including mitral valve replacement, aortic valve replacement (AVR), and double valve replacement (DVR).A total of 726 patients were studied over a 3-year period (2011-2013) after the implementation of IAD and were compared with 919 patients during the previous 36-month period (January 2008-December 2010). The method of small-volume retrograde autologous priming, strict blood transfusion standard together with IAD constituted a progressive blood-saving strategy.Baseline characteristics and preoperative information showed no statistically significant difference between IAD group and non-IAD group. Most of the postoperative morbidities are statistically the same in the 2 groups. Chest tube output (415.2 vs 1029.8 mL, P < 0.001) and postoperative respiratory failure (5.9% vs 8.6%, P = 0.039) favored the IAD group, whereas hematocrit levels were more favorable in the non-IAD group (30.3% vs 33.0% at the end of the operation, P < 0.001; 30.4% vs 31.5% at the time of discharge). The use of blood product transfusion was higher in the non-IAD group (22.6% vs 43.3%, P < 0.001). Binary multivariate logistic regression analysis showed that high age, non-IAD, DVR surgery, and absent smoking history are associated with a higher risk of intra-/postoperative blood transfusion.Blood conservation is effective and safe in cardiac valve replacement surgeries. The use of intraoperative autologous donation can lead to improved outcomes including a significantly lower rate of intra-/postoperative blood transfusion and postoperative complications.


Assuntos
Procedimentos Médicos e Cirúrgicos de Sangue/normas , Doenças das Valvas Cardíacas/cirurgia , Implante de Prótese de Valva Cardíaca/métodos , Guias de Prática Clínica como Assunto , Feminino , Implante de Prótese de Valva Cardíaca/normas , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
10.
Nat Neurosci ; 19(6): 855-61, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27159800

RESUMO

The orbitofrontal cortex plays a central role in good-based economic decisions. When subjects make choices, neurons in this region represent the identities and values of offered and chosen goods. Notably, choices in different behavioral contexts may involve a potentially infinite variety of goods. Thus a fundamental question concerns the stability versus flexibility of the decision circuit. Here we show in rhesus monkeys that neurons encoding the identity or the subjective value of particular goods in a given context 'remap' and become associated with different goods when the context changes. At the same time, the overall organization of the decision circuit and the function of individual cells remain stable across contexts. In particular, two neurons supporting the same decision in one context also support the same decision in different contexts. These results demonstrate how the same neural circuit can underlie economic decisions involving a large variety of goods.


Assuntos
Comportamento de Escolha/fisiologia , Tomada de Decisões/fisiologia , Lobo Frontal/fisiologia , Rede Nervosa/fisiologia , Neurônios/fisiologia , Animais , Macaca mulatta , Masculino
11.
J Immunol Res ; 2015: 742535, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26491701

RESUMO

Henoch-Schönlein purpura (HSP) is a common systemic small vessel vasculitis in children with disorder autoimmune responses. T follicular helper (TFH) cells play crucial roles in regulating immune responses. The aim of our study was to investigate the probable role of TFH cells in the pathogenesis of children with HSP. In this study, the frequency of circulating CXCR5(+)CD4(+)TFH cells with inducible costimulator (ICOS) expression in the children with acute HSP was significantly higher than that in healthy controls (HCs) but not CXCR5(+)CD4(+)TFH cells with programmed death-1 (PD-1) expression. Moreover, serum levels of IL-21 and IL-6 cytokines, IgA, and C3 in HSP children were also significantly higher than those in HCs. A positive correlation was observed between the frequencies of circulating ICOS(+)CXCR5(+)CD4(+)TFH cells and the serum IL-21 or IgA levels of acute HSP children, respectively. Additionally, the mRNA expression levels of interleukin- (IL-) 21, IL-6, and transcriptional factors (B-cell lymphoma-6, Bcl-6) were also significantly increased in peripheral blood from acute HSP children compared to HCs. Taken together, these findings suggest that TFH cells and associated molecules might play critical roles in the pathogenesis of HSP, which are possible therapeutic targets in HSP children.


Assuntos
Contagem de Linfócito CD4 , Púrpura de Schoenlein-Henoch/sangue , Púrpura de Schoenlein-Henoch/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Doença Aguda , Biomarcadores , Estudos de Casos e Controles , Criança , Pré-Escolar , Citocinas/sangue , Citocinas/genética , Citocinas/metabolismo , Feminino , Expressão Gênica , Humanos , Imunoglobulina A/sangue , Imunoglobulina A/imunologia , Imunofenotipagem , Masculino , Púrpura de Schoenlein-Henoch/diagnóstico , Púrpura de Schoenlein-Henoch/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Pele/patologia , Linfócitos T Auxiliares-Indutores/metabolismo
12.
Int J Biol Sci ; 11(2): 220-9, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25561904

RESUMO

BACKGROUND: Idiopathic thrombocytopenic purpura (ITP) is a primary autoimmune disease with a decreased platelet count caused by platelet destruction mediated mainly by platelet antibodies. T follicular helper (TFH) cells have demonstrated important roles in autoimmune diseases. The aim of this study is to explore the might role of TFH cells in the patients of ITP. METHODS: Twenty-three ITP patients and 12 healthy controls (HC) were enrolled in this study. The frequency of circulating TFH cells in both the patients and HC was analyzed by flow cytometry. Serum interleukin (IL)-21 and IL-6 levels were measured using ELISA, and platelet antibodies were tested using a solid phase technique. Additionally, IL-21, IL-6, Bcl-6 and c-Maf mRNA expressions in peripheral blood mononuclear cells (PBMCs) were detected using real-time PCR. RESULTS: The percentages of circulating CXCR5(+) CD4(+)TFH cells with ICOS(high) or PD-1(high) expression were significantly higher in the ITP patients than in the HC. Moreover, the frequencies of circulating CXCR5(+) CD4(+)TFH cells with inducible costimulator (ICOS)(high) or programmed death-1 (PD-1)(high) expression were notably higher in ITP with platelet-antibody-positive ( ITP (+) ) patients than in ITP with platelet-antibody-negative ( ITP (-) ) patients and HC, as were the serum IL-21 and IL-6 levels (significant). Moreover, a positive correlation was found between the CXCR5(+)CD4(+)TFH cells with ICOS(high) or PD-1(high) expression and the serum IL-21 levels of ITP (+) patients. Additionally, the mRNA expression levels of IL-21, IL-6, Bcl-6 and c-Maf were significantly increased in ITP patients, especially in ITP (+) patients. CONCLUSIONS: This study demonstrated TFH cells and effector molecules might play an important role in the pathogenesis of ITP, which are possible therapeutic targets in ITP patients.


Assuntos
Púrpura Trombocitopênica Idiopática/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Adulto , Doenças Autoimunes/sangue , Doenças Autoimunes/imunologia , Doenças Autoimunes/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Citometria de Fluxo , Humanos , Interleucina-6/sangue , Interleucinas/sangue , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , Púrpura Trombocitopênica Idiopática/sangue , Púrpura Trombocitopênica Idiopática/metabolismo , Receptores CXCR5/genética , Receptores CXCR5/metabolismo , Linfócitos T Auxiliares-Indutores/metabolismo
13.
Oncol Lett ; 7(1): 131-136, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24348834

RESUMO

Epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF), and their receptors, epithelial growth factor receptor (EGFR) and bFGF receptor (bFGFR), are frequently overexpressed in high-grade gliomas. In the present study, the EGF and bFGF levels in U251 glioblastoma cell culture supernatants were determined by ELISA, and enhanced green fluorescent protein (EGFP)-labeled recombinant lentiviral expression vectors with small interfering RNA targeting the EGFR and bFGFR genes were constructed. The mRNA expression levels of EGFR, bFGFR, cluster of differentiation (CD)133, glial fibrillary acidic protein (GFAP), tubulin-ß3 (TUBB3) and myelin basic protein (MBP) were determined using quantitative polymerase chain reactions in U251 cells prior to and following silencing of the EGFR and/or bFGFR genes. Prior to silencing, the U251 cells secreted EGF and bFGF, and expressed EGFR, bFGFR, CD133, GFAP, TUBB3 and MBP mRNA. Subsequent to silencing the EGFR and/or bFGFR gene, CD133 mRNA expression decreased and GFAP and TUBB3 mRNA expression increased. Silencing the EGFR and FGFR genes acted synergistically to downregulate CD133 expression. The downregulation of CD133 mRNA expression and the upregulation of GFAP and TUBB3 mRNA expression were not significantly different when blocking the EGFR and FGFR pathways. These results indicate that autocrine or paracrine EGF and/or FGF mechanisms exist in U251 cells. Knocking down the EGFR and/or FGFR genes downregulates CD133 mRNA expression and facilitates glial and neuronal differentiation in U251 cells.

14.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 23(11): 650-2, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22093308

RESUMO

OBJECTIVE: To examine the effect of a newly-developed tracheal detecting-bulb (TDB) in preventing the spray of contaminants in patients' exhalent during emergency endotracheal intubation procedure. METHODS: 62 emergency patients (male or female, age 21-73 years, weight 47-83 kg, with different levels of consciousness and spontaneous breathing) were randomly divided into two groups (n =31 each) to receive endotracheal intubation by an anesthesiologist with more than 17 years of experience, with (TDB group) or without (control group) a TDB connected to the tracheal tubing. Records were taken regarding the patient's coughing, expansion / contraction of the TDB, the operator's feeling of the patient's exhalent on his face / neck and the contamination of these areas by blood / secretions from the patients in the due course. RESULTS: In TDB group, expansion / contraction of TDB occurred in all cases, but the operator felt no exhalent in any of them. In 9 cases the patients coughed but the operator was contaminated only in 2 cases. While in control group, the patients' exhalent was felt in 16 cases. Coughing were recorded in 7 cases, and the operator was contaminated in 11 cases. In comparison with the control group, TDB group had significantly lower(both P < 0.05), incidence in feeling the exhalent (0 vs. 16), and contamination by blood or secretions (2 vs. 11) although the two groups had no significant difference in the incidence of coughing (9 vs. 7, P > 0.05). CONCLUSION: TDB can play an important role in blocking the spray of contaminants in patients' exhalent during endotracheal intubation.


Assuntos
Intubação Intratraqueal/instrumentação , Intubação Intratraqueal/métodos , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Equipamentos de Proteção , Adulto Jovem
15.
Hepatobiliary Pancreat Dis Int ; 5(3): 468-70, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16911953

RESUMO

BACKGROUND: There is no report on case of severe acute hyperlipidemic pancreatitis after treatment of Sheehan's syndrome. METHODS: A 32-year-old female patient was diagnosed as having acute hyperlipidemic pancreatitis after treatment of Sheehan's syndrome, and treated with diet and lipid-lowering agents in early stage. RESULTS: Abdominal pain and fever of the patient resolved within a few days. She was subjected to diet and oral lipid-lowering therapy on the 4th day after admission. The disease did not recur during the follow-up for more than one year. CONCLUSIONS: Estrogen replacement therapy should be prescribed for Sheehan's syndrome. The serum level of triglyceride should be monitored and treatment should be given to prevent severe acute pancreatitis. Lipid-lowering therapy in early stage is the key step towards a complete recovery.


Assuntos
Hipertrigliceridemia/complicações , Hipopituitarismo/complicações , Pancreatite/complicações , Doença Aguda , Adulto , Feminino , Humanos , Tomografia Computadorizada por Raios X
16.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 34(6): 534-7, 2005 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-16331816

RESUMO

OBJECTIVE: To investigate the telomerase activity and the telomere length in CD4(+), CD8(+) and CD19(+) lymphocytes from patients with systemic lupus erythematosus (SLE). METHODS: Telomerase activity of CD4(+), CD8(+) and CD19(+) cells from patients with SLE and normal controls was examined by telomeric repeats amplification protocol. Telomere length in those cells was measured by Southern blot method. RESULT: CD4(+), CD8(+) and CD19(+) cells in patients with SLE showed high telomerase activity, but only telomerase activity of CD19(+) cells was significantly higher than that in controls. The length of telomere in CD4(+) and CD8(+) cells was significantly shorter than that in controls, but not in CD19(+) cells. CONCLUSION: Higher telomerase activity in CD19(+) cells and shortened telomere length in CD4(+) and CD8(+) cells of patients with SLE may be associated with pathogenesis of SLE.


Assuntos
Linfócitos T CD4-Positivos/enzimologia , Linfócitos T CD8-Positivos/enzimologia , Lúpus Eritematoso Sistêmico/imunologia , Telomerase/metabolismo , Telômero/genética , Adolescente , Adulto , Antígenos CD19/imunologia , Feminino , Humanos , Lúpus Eritematoso Sistêmico/enzimologia , Linfócitos/enzimologia , Masculino , Pessoa de Meia-Idade
17.
Hepatobiliary Pancreat Dis Int ; 4(2): 299-301, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15908334

RESUMO

BACKGROUND: There is a controversy about the risk of injury to the branch of the middle hepatic vein during laparoscopic cholecystectomy. This study was conducted to further investigate the relationship between the gallbladder bed and the branch of the middle hepatic vein. METHODS: Color Doppler ultrasound was used to examine the anatomical relationship between the gallbladder bed and the branches of the middle hepatic vein in 143 healthy volunteers. RESULTS: Not all the middle hepatic vein extended close to the gallbladder bed, the branches and gallbladder beds in 23 subjects were not in the same plane during ultrasound scanning. In 21 of the 143 subjects the branch of the middle hepatic vein was completely adherent to the gallbladder bed with a diameter ranging from 1.2 mm to 3.6 mm. In 10 subjects the branches of the middle hepatic vein traversed approximately 1.0 mm from the gallbladder bed with a diameter ranging from 1.6 mm to 3.0 mm. CONCLUSIONS: In most subjects the branch of the middle hepatic vein and the gallbladder bed are well separated. Only patients with large branches of the middle hepatic vein close to the gallbladder bed are at risk of hemorrhage during laparoscopic cholecystectomy.


Assuntos
Vesícula Biliar/irrigação sanguínea , Vesícula Biliar/diagnóstico por imagem , Veias Hepáticas/diagnóstico por imagem , Ultrassonografia Doppler em Cores , Colecistectomia Laparoscópica/métodos , Estudos de Coortes , Feminino , Doenças da Vesícula Biliar/diagnóstico por imagem , Doenças da Vesícula Biliar/cirurgia , Humanos , Masculino , Complicações Pós-Operatórias/prevenção & controle , Fatores de Risco , Sensibilidade e Especificidade
18.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 25(3): 244-7, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15842148

RESUMO

OBJECTIVE: To investigate the apoptosis inducing effect of tea polyphenols (TPP) on human lung cancer cell (LCC) and its associative mechanism. METHODS: The apoptosis inducing effect of TPP on LCC in vitro, and its influence on expression of the related gene were determined by MTT assay, laser scanning confocal microscopy and flow cytometry. RESULTS: TPP in different concentration (50,100,200 and 400 microg/ml) had dose-dependent inhibitory effect on LCC, the inhibitory rate was 28.69+/-1.27% ,46. 19+/-1.79% ,64.61+/-1.29%, 75.90+/-1.96%, respectively. The inhibited LCC were blocked in (G0/G1 phase, and could not transferred to S and G2/ M phase of cell cycle. Meanwhile, TPP could induce apoptosis of LCC, the apoptotic rate being 4.76+/-0.11 %, 5.78+/-0.38 %, 10.06+/-0.67 %, 24.44+/-0.44 %, respectively. Morphologic changes of cells were seen in laser scanning confocal microscopy observation. Compared to the control group, intracellular Ca2+ concentration, Annexin V expression, phospatase and tensin homologe deleted on chromosome ten (PTEN) protein and expression gradually increased, while Cyclin D1 protein expression gradually decreased in the TPP treated groups along with the increasing of TPS concentration. CONCLUSION: TTP can induce LCC apoptosis, the mechanism is related to the change of intracellular Ca2+ concentration, PTEN protein and Cyclin D1 protein expression.


Assuntos
Apoptose/efeitos dos fármacos , Flavonoides/farmacologia , Neoplasias Pulmonares/patologia , Fenóis/farmacologia , Chá/química , Anexina A5/biossíntese , Antineoplásicos Fitogênicos/farmacologia , Ciclina D1/biossíntese , Relação Dose-Resposta a Droga , Humanos , PTEN Fosfo-Hidrolase , Monoéster Fosfórico Hidrolases/biossíntese , Polifenóis , Células Tumorais Cultivadas , Proteínas Supressoras de Tumor/biossíntese
19.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 11(3): 274-7, 2003 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-12844412

RESUMO

In order to observe the expression of Fas, FasL and Bcl-2 and apoptosis of bone marrow CD34(+) cells in patients with myelodysplastic syndrome (MDS), and to explore the relation between the expression of these antigens and apoptosis, the expression of Fas, FasL and Bcl-2 and apoptosis of bone marrow CD34(+) cell were evaluated by flow cytometry in 26 patients with MDS including 9 cases of refactory anemia (RA), 1 case of RA with ringed sideroblasts (RAS), 9 cases of RA with excess blasts (RAEB) and 7 cases of RAEB in transformation (RAEB-t), 10 patients with acute myeloid leukemia (AML) and 6 control patients with normal bone marrow. The results showed that the expression of Fas and FasL of CD34(+) cells significantly increased in all types of MDS patients compared with control group (P < 0.01). The expression of Bcl-2 on CD34(+) cells in RAEB and RAEB-t patients was much higher as compared with that in control group (P < 0.01), but there was no significant difference between RA/RAS patients and control group (P > 0.05). The expression rates of Fas on CD34(+) cells were almost identical in all kinds of MDS, but there was significant difference on the expression of Bcl-2 (RA/RAS < RAEB < RAEB-t). Apoptosis of CD34(+) cells significantly increased in RA/RAS and RAEB patients compared with control group (P < 0.01), but there was no difference between RAEB-t and control group. Moreover, apoptosis of CD34(+) cells in control much higher than that in AML group (P < 0.01). There was no correlation between the expression of Fas or FasL and apoptosis on CD34(+) cell of MDS patients. Nevertheless, there was a negative correlation between the expression of Bcl-2 and apoptosis. It is concluded that apoptosis of CD34(+) cells was affected by a lot of factors in MDS, in which Bcl-2 is an important factor of depressing apoptosis. During the progress from MDS to AML, apoptosis changes from overgoing to deficiency in CD34(+) cell.


Assuntos
Apoptose , Biomarcadores/análise , Células da Medula Óssea/metabolismo , Síndromes Mielodisplásicas/sangue , Doença Aguda , Adulto , Anemia Refratária com Excesso de Blastos/sangue , Anemia Refratária com Excesso de Blastos/patologia , Antígenos CD34/sangue , Células da Medula Óssea/imunologia , Células da Medula Óssea/patologia , Proteína Ligante Fas , Feminino , Citometria de Fluxo , Humanos , Leucemia Mieloide/sangue , Leucemia Mieloide/patologia , Masculino , Glicoproteínas de Membrana/biossíntese , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/patologia , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Receptor fas/biossíntese
20.
Zhong Xi Yi Jie He Xue Bao ; 1(3): 184-6, 2003 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-15339556

RESUMO

OBJECTIVE: To observe the clinical effect of cinobufacini injection in treating moderate and advanced primary liver cancer (PLC). METHODS: One hundred patients with moderate and advanced PLC were randomly divided into cino-treated group (50 patients) and control group (50 patients). The quality of life, tumor size, some changes of laboratory tests, and survival time were observed. RESULTS: The progressive rate of cino-treated group (18%) was lower than that of the control group (32%). The quality of life of the cino-treated group (80%) was better than that of the control group (72%), but without statistical significance. The survival rate of >12 months of the cino-treated group (30%) was higher than that of the control group (18%). The patients' liver function such as serum total bilirubin and ALT decreased obviously in the cino-treated group while increased a lot in the control group. The level of AFP increased after treatment with statistical significance in the control group while there was no statistical significance in the cino-treated group. CONCLUSION: Cinobufacini injection can not only inhibit the proliferation of cancer, but also protect liver function, improve quality of life and prolong survival time.


Assuntos
Carcinoma Hepatocelular/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Neoplasias Hepáticas/tratamento farmacológico , Adulto , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/patologia , Medicamentos de Ervas Chinesas/administração & dosagem , Feminino , Humanos , Injeções Intravenosas , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Qualidade de Vida , Taxa de Sobrevida , Resultado do Tratamento
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