Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 856
Filtrar
1.
IEEE Trans Cybern ; PP2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33417579

RESUMO

In this text, a membership function derivatives (MFDs) extrema-based method is proposed to relax the conservatism both in stability analysis and synthesis problems of Takagi-Sugeno fuzzy systems. By the designed algorithm, the nonpositiveness of the MFDs extrema is conquered. For an open-loop system, based on certain information of the MFs and derivatives, a series of convex stability conditions is derived. Then, an extremum-based construction method is adopted to involve the MF information. For the shape of MFDs, a coordinate transformation algorithm is proposed to involve it in the stability conditions to achieve local stable effects. For a state-feedback control system, conditions guaranteeing the stability and robustness are listed. Finally, simulation examples and comparisons are carried out to clarify the conservatism reduction results of the raised method.

2.
Aging (Albany NY) ; 122021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-33461173

RESUMO

RNA binding proteins (RBPs) play significant roles in the development of tumors. However, a comprehensive analysis of the biological functions of RBPs in clear cell renal cell carcinoma (ccRCC) has not been performed. Our study aimed to construct an RBP-related risk model for prognosis prediction in ccRCC patients. First, RNA sequencing data of ccRCC were downloaded from The Cancer Genome Atlas (TCGA) database. Three RBP genes (EIF4A1, CARS, and RPL22L1) were validated as prognosis-related hub genes by univariate and multivariate Cox regression analyses and were integrated into a prognostic model by least absolute shrinkage and selection operator (LASSO) Cox regression analysis. According to this model, patients with high risk scores displayed significantly worse overall survival (OS) than those with low risk scores. Moreover, the multivariate Cox analysis results indicated that risk score, tumor grade, and tumor stage were significantly correlated with patient OS. A nomogram was constructed based on the three RBP genes and showed a good ability to predict outcomes in ccRCC patients. In conclusion, this study identified a three-RBP gene risk model for predicting the prognosis of patients, which is conducive to the identification of novel diagnostic and prognostic molecular markers.

3.
Future Oncol ; 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33401983

RESUMO

Aim: We performed an updated meta-analysis to evaluate the efficacy and safety of lenvatinib in cancer patients. Materials & methods: Databases were searched to identify relevant trials. Data were extracted to evaluate overall survival, progression-free survival, overall response rate and grade ≥3 adverse events. Results: The pooled analysis demonstrated that lenvatinib significantly improved progression-free survival (hazard ratio: 0.43; 95% CI: 0.23-0.80; p = 0.008), overall survival (hazard ratio: 0.85; 95% CI: 0.75-0.97; p = 0.013) and overall response rate (relative risk: 6.89; 95% CI: 2.22-21.36; p = 0.001) compared with control therapy. However, the use of lenvatinib can increase the risk of severe infection. Conclusion: Lenvatinib-containing regimens are associated with better progression-free survival, overall survival and overall response rate, but can induce severe infection.

4.
Am J Surg ; 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33454024
5.
Hepatol Int ; 2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33460002

RESUMO

BACKGROUND AND AIMS: Many models have been developed to predict liver-related events (LRE) in chronic hepatitis B, few focused on compensated HBV-induced cirrhosis. We aimed to describe the incidence of LRE and to determine independent risk predictors of LRE in compensated HBV-induced cirrhosis patients receiving antiviral therapy using routinely available parameters. METHODS: Prospective cohorts of treatment-naïve adults with compensated HBV-induced cirrhosis were enrolled. Patients were treated with entecavir (ETV) or ETV + thymosin-alpha1 (Thy-α1) or lamivudine (LAM) + adefovir (ADV). Data were collected at baseline and every 6 months. LRE was defined as development of decompensation, HCC or death. RESULTS: Totally 937 patients were included, 608 patients treated with ETV, 252 with ETV + Thy-α1, and 77 with LAM + ADV. After a median follow-up of 4.5 years, 88 patients developed LRE including 48 with HCC. The cumulative incidence of LRE at year 1, 3, and 5 was 2.1%, 7.0%, and 12.7%, respectively, and was similar for three treatment groups. All models using variables at month 6 or 12 had better fit than models using baseline values. The best model for prediction of LRE used PLT, GGT, and AFP at month 6 [AUC: 0.762 (0.678-0.814)], for hepatic decompensation-PLT, LSM and GGT at month 12 (AUC: 0.834 (0.675-0.919)), and for HCC-AFP and GGT at month 6 [AUC 0.763 (0.691-0.828)]. All models had negative predictive values of 94.0-98.8%. CONCLUSION: Models using on-treatment variables are more accurate than models using baseline variables in predicting LRE in patient with compensated HBV-induced cirrhosis receiving antiviral therapy. ClincialTrials.gov number NCT01943617, NCT01720238, NCT03366571, NCT02849132.

6.
Insect Sci ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33399267

RESUMO

The invasive pest whitefly (Bemisia tabaci) is a complex species, of which Middle East-Minor Asia 1 (MEAM1) and Mediterranean (MED) are the two most damaging members. Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED, and this difference in performance is associated with glucosinolate (GS) content. Some insects can modify GS using glucosinolate sulfatase (SULF), the activity of which is regulated by sulfatase modifying factor 1 (SUMF1); therefore, to increase our understanding of different performances of MEAM1 and MED on cabbage plants, we identified and compared nine putative SULFs and one SUMF in MEAM1 and MED. We found that the lengths of two genes, BtSulf2 and BtSulf4, differed between MEAM1 and MED. The messenger RNA levels of BtSulf4 increased more than 20-fold after MEAM1 and MED adults were exposed to GS, but BtSulf2 expression was only induced by GS in MEAM1. Knockdown of BtSulf2 and BtSulf4 in MEAM1 resulted in a substantial increase in the mortality of GS-treated adults but not in MED. These results indicate that differences in BtSulf2 and BtSulf4 sequences and/or expression may explain why MEAM1 performs better than MED on cabbage. Our results provide a basis for future functional research on SULF and SUMF in B. tabaci.

7.
Audiol Neurootol ; 26(1): 11-16, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32535600

RESUMO

OBJECTIVE: To assess the efficacy of the combination of hyperbaric oxygen (HBO) and pharmacological treatment in patients with idiopathic sudden sensorineural hearing loss (ISSNHL) and define patients amenable for HBO therapy. METHODS: Prospective, randomized, trial involving 136 cases with unilateral ISSNHL that were randomly divided into 2 groups: the pharmacological treatment (P) group and HBO + pharmacological treatment (HBO+P) group, which received additional HBO for 14 days besides the pharmacological treatments. Pure tone audiometry gain larger than 15 dBHL was defined as success, and the success rate of each group was calculated. RESULTS: The overall success rate of the HBO+P group and the P group is 60.6% (40/66) and 42.9% (30/70), respectively (p < 0.05). Furthermore, patients with mild-moderate baseline hearing loss, aged ≤50 years, receiving treatment in ≤14 days, or without accompanied dizziness/vertigo in the HBO+P group had higher success rate than the P group (p < 0.05). CONCLUSIONS: HBO combined with pharmacological treatments leads to better hearing recovery than pharmacological treatments alone.

9.
Liver Int ; 41(2): 369-377, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33277803

RESUMO

BACKGROUND & AIMS: Non-invasive assessment criteria to rule out high-risk varices (HRV) in compensated hepatitis B virus (HBV) cirrhosis on antiviral therapy remains unclear. METHODS: HBV-related compensated cirrhotic patients who underwent screening endoscopy during antiviral therapy were enrolled and randomly divided into the derivation and validation sets. HRV were defined as medium to large varices or small varices with red signs. Univariate and multivariate logistic analysis were used to determine the parameters associated with HRV. RESULTS: A total of 436 HBV-related compensated cirrhotic patients screened for varices were enrolled, the median duration of antiviral therapy was 4 years (IQR: 2.5-5.5 years). In the derivation set (N = 290, 17.2% with HRV), only platelet (PLT) count (OR = 0.972, 95% CI 0.961-0.984, P < .05) was independently associated with HRV, whereas liver stiffness measurement was not associated with the presence of HRV. With a PLT count cut-off value of 105 × 109 /L, unnecessary endoscopies could be spared in 56.9% patients, with a 3.6%. risk of missing HRV. In the validation cohort (N = 146, 16.4% with HRV), the proportion of patients that could safely spare endoscopies (61.0%) identified by this PLT count cut-off value was higher than that obtained by using Baveno VI criteria (34.9%), with an acceptable risk of missing HRV (3.4%). CONCLUSION: Compared with the 'Baveno VI criteria or beyond' criteria, PLT count higher than 105 × 109 /L could safely spare more screening endoscopies without increasing the risk of missing HRV in patients with HBV-related compensated cirrhosis on antiviral therapy.

10.
Acad Radiol ; 28(1): 49-57, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32113879

RESUMO

OBJECTIVES: The relationship between the 18FDG PET-CT maximum standard uptake value (SUVmax) and the type of lung adenocarcinoma is still not established. The aim of this study was to investigate the relationship between SUVmax value and histological grade and pathological subtype of lung adenocarcinoma, and to determine the optimum SUVmax cutoffs for distinguishing different histological grades. MATERIALS AND METHODS: The data of 618 lung adenocarcinoma patients were retrospectively analyzed. The relationship between SUVmax measured on preoperative 18FDG-PET-CT and the histological grade and pathological subtype was examined. The Kruskal-Wallis test was used to compare differences among groups, and the Bonferroni-Dunn test for pairwise comparison among groups. ROC analysis was applied to determine the optimal cut-off values for distinguishing different groups. In addition, the cut-off value was verified in an independent cohort of 85 consecutive lung adenocarcinoma cases. RESULTS: The SUVmax was significantly different between the low, intermediate, and high-grade groups(p < .001). SUVmax value increased with increase in the degree of malignancy. The optimal cut-off value for identifying low-grade tumors was 2.01 (sensitivity 90.4%, specificity 86.9%, area under the curve [AUC] = 0.928, 95% confidence interval: 0.91-0.95; p < .001). The optimal cutoff SUVmax value for identifying high-grade tumors was 7.41 (sensitivity 79.8%, specificity 73.5%, AUC = 0.830, 95% confidence interval: 0.79-0.87; p < .001). The validation experiment showed that the coincidence rate was 88.89% in the low-level group, 64.15% in the middle-level group, and 78.57% in the high-level group. CONCLUSION: SUVmax can be used to predict pathological subtype and histological grade of lung adenocarcinoma. Thus, 18FDG PET-CT can serve as a noninvasive tool for precise diagnosis and help in the preoperative formulation of patient-specific treatment strategies.

11.
Appl Microbiol Biotechnol ; 105(1): 1-12, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33201273

RESUMO

Small regulatory RNAs (sRNAs) finely control gene expression in prokaryotes and synthetic sRNA has become a useful high-throughput approach to tackle current challenges in metabolic engineering because of its many advantages compared to conventional gene knockouts. In this review, we first focus on the modular structures of sRNAs and rational design strategies of synthetic sRNAs on the basis of their modular structures. The wide applications of synthetic sRNAs in bacterial metabolic engineering, with or without the aid of heterogeneously expressed Hfq protein, were also covered. In addition, we give attention to the improvements in implementing synthetic sRNAs, which make the synthetic sRNA strategy universally applicable in metabolic engineering and synthetic biology. KEY POINTS: • Synthetic sRNAs can be rationally designed based on modular structures of natural sRNAs. • Synthetic sRNAs were widely used for metabolic engineering in various microorganisms. • Several technological improvements made the synthetic sRNA strategy more applicable.

12.
Fitoterapia ; 148: 104777, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33238199

RESUMO

Three new indole alkaloids, ophiorrhines C-D (1-3), together with one known analogue, have been isolated from the plant of Ophiorrhiza cantoniensis Hace. The structures of the new alkaloids with the absolute configurations were elucidated by means of spectroscopic methods, electronic circular dichroism (ECD) and calculated nuclear magnetic resonance (NMR) with DP4+ analysis. Compounds 1 and 2 exhibited certain activity to Con-A induced T cell proliferation, and 1 exhibited good inhibition on LPS-induced B cell proliferation with an IC50 value of 8.7 µM.

13.
Cancer Lett ; 497: 28-40, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33069768

RESUMO

Angiogenesis factors are widely known to promote tumor growth by increasing tumor angiogenesis in the tumor microenvironment, however, little is known whether their intracellular function is involved in tumorigenesis. Here we show that AGGF1 acts as a tumor suppressor by regulating p53 when acting inside tumor cells. AGGF1 antagonizes MDM2 function to inhibit p53 ubiquitination, increases the acetylation, phosphorylation, stability and expression levels of p53, activates transcription of p53 target genes, and regulates cell proliferation, cell cycle, and apoptosis. AGGF1 also interacts with p53 through the FHA domain. Somatic AGGF1 variants in the FHA domain in human tumors, including p.Q467H, p.Y469 N, and p.N483T, inhibit AGGF1 activity on tumor suppression. These results identify a key role for AGGF1 in an AGGF1-MDM2-p53 signaling axis with important functions in tumor suppression, and uncover a novel trans-tumor-suppression mechanism dependent on p53. This study has potential implications in diagnosis and therapies of cancer.

14.
J Nanosci Nanotechnol ; 21(2): 1278-1285, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183473

RESUMO

Schizophrenia is one of the most common central nervous system diseases, which is caused by abnormal discharge of neurons in the brain. Its occurrence and development are affected by both genetic and environmental factors. The variation of gene level can affect the development of schizophrenia and the treatment of prognosis by affecting the susceptibility, clinical phenotype and drug response. At present, the research results of susceptibility genes screened by candidate gene association research are not consistent. The method of gene recognition on DNA was studied by QCM and nano gold composite. By using this method, the enantioselective recognition of cysteine on cyclodextrin self-assembled membrane was studied. In this study, EAAT1 gene, which is highly expressed in astrocytes, was used as a candidate gene to analyze the relationship between polymorphism and schizophrenia. The experimental results show that the introduction of nano gold can significantly improve the sensing signal, detection sensitivity and gene differentiation. In addition, this study suggested that EAAT1 gene might be a susceptibility gene of schizophrenia in the population. The results showed that a common SNP allele rs1030239-g was the risk factor (83.8% vs. 79.2%, P = 0.00067, or = 1.35, 95% CI = 1.08-1.69). The results showed that A-T-G increased the risk of schizophrenia.

15.
Dose Response ; 18(4): 1559325820974543, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33293908

RESUMO

Sarcopenia is often regarded as an early sign of weakness and is the core element of muscle weakness in elderly individuals. Sarcopenia is closely related to the reduction of exercise, and elderly individuals often suffer from decreased muscle mass and function due to a lack of exercise. At present, studies have confirmed that resistance and aerobic exercise are related to muscle mass, strength and fiber type and to the activation and proliferation of muscle stem cells (MuSCs). Increasing evidence shows that microRNAs (miRNAs) play an important role in exercise-related changes in the quantity, composition and function of skeletal muscle. At the cellular level, miRNAs have been shown to regulate the proliferation and differentiation of muscle cells. In addition, miRNAs are related to the composition and transformation of muscle fibers and involved in the transition of MuSCs from the resting state to the activated state. Therefore, exercise may delay sarcopenia in elderly individuals by regulating miRNAs in skeletal muscle. In future miRNA-focused treatment strategies, these studies will provide valuable information for the formulation of exercise methods and will provide useful and targeted exercise programs for elderly individuals with sarcopenia.

17.
Med Res Rev ; 2020 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-33377531

RESUMO

Nowadays, human microbiome research is rapidly growing and emerging evidence has witnessed the critical role that oral microbiome plays in the process of human health and disease. Oral microbial dysbiosis has been confirmed as a contributory cause for diseases in multiple body systems, ranging from the oral cavity to the gastrointestinal, endocrine, immune, cardiovascular, and even nervous system. As research progressing, oral microbiome-based diagnosis and therapy are proposed and applied, which may represent potential drug targets in systemic diseases. Recent studies have uncovered the possible association between periodontal disease and prostatic disease, suggesting new prevention and therapeutic treatment for the disease by targeting periodontal pathogens. Thus, we performed this review to first explore the association between the oral microbiome and prostatic disease, according to current knowledge based on published articles, and then mainly focus on the underlying molecular and cellular mechanisms and the potential prevention and treatment derived from these mechanistic studies.

18.
Artigo em Inglês | MEDLINE | ID: mdl-33285263

RESUMO

Anhedonia is a core characteristic of depression, the amelioration of which accounts for depressive symptom improvement. Electroconvulsive therapy (ECT) has been shown remarkable antidepressive effect, however, less is known about the effect of ECT on anhedonia and its underlying neural mechanism. Herein, we investigated local and global intrinsic brain functional alterations during the resting state in 46 patients with pre- and post-ECT major depressive disorder using the amplitude of low-frequency fluctuations (ALFF) and degree centrality (DC) approach. Functional connectivity (FC) was also calculated between nodes with significant local and global intrinsic brain functional alterations. The severity of anhedonia and depression was assessed with the Temporal Experience of Pleasure Scale and Hamilton Depression Rating Scale, respectively. The relationship between the change in anhedonia and depressive symptoms and brain functional alterations was determined. Increased ALFF and DC were observed in the bilateral dorsal medial prefrontal cortex (dmPFC), right dorsal lateral prefrontal cortex (dlPFC), left orbitofrontal cortex, and right orbitofrontal cortex (ROFC) after ECT. Correlational analysis between the change in anhedonia and ALFF had positive results in the dmPFC. Similarly, there was a positive correlation between the change in anhedonia and change in DC in the dmPFC, right dlPFC, ROFC, and middle frontal gyrus. Furthermore, there was a significant relationship between the change in anhedonia and altered dmPFC-dlPFC FC. These results revealed that amelioration of anhedonia may be associated with intrinsic neural activity alteration in the reward-related circuit and executive control network following ECT.

19.
Ying Yong Sheng Tai Xue Bao ; 31(11): 3859-3868, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33300737

RESUMO

In order to provide scientific guidance for soil quality evaluation and optimum management of flower and seedling industry, we investigated the characteristics of soil animal community with different garden plants and various planting periods in Wenjiang District, Chengdu. A total of 10258 soil animals belonging to 26 orders and 78 families were captured in four sampling times. There were significant differences in the taxonomic richness in the plots with different garden plants, generally highest in plots with Loropetalum chinense var. rubrum or Ginkgo biloba and lowest in plot with Zoysia japonica. The taxonomic richness was lower in the plots with different garden plants than the control. Taxonomic richness and abundance of soil fauna in Osmanthus fragrans plot did not change across sampling seasons. The abundance but not taxonomic richness of soil fauna in other plots had obvious seasonal variations. Different garden plants and various planting periods significantly influenced soil faunal diversity indices. Density-group index (DG) and Margalef richness index (D) in G. biloba plot, as well as Shannon diversity index (H) and D index in Z. japonica plot decreased significantly with the increases of planting period. The DG and D indices of soil animals in O. fragrans plot increased significantly with increasing planting period. The indices of soil animal diversity in L. chinense var. rubrum plot did not change regularly with planting period. The DG, D, and H indices were lowest in O. fragrans plot with different planting periods. Results of hierarchical clustering and canonical correlation analyse (CCA) indicated that garden plant species had stronger effects on the habitat than planting period. Responses of soil fauna to various habitats were different, with available P and soil pH having stronger effects on soil fauna. Our results indicated that soil animal community shifted with the changes of garden plant and planting period as well as management and cultivation methods. Excessive human interference and monoculture had negative effects on soil animal community and caused soil degradation.


Assuntos
Jardins , Solo , Animais , China , Ecossistema , Humanos , Plantas
20.
Ying Yong Sheng Tai Xue Bao ; 31(11): 3915-3924, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33300743

RESUMO

Based upon the review of the status of nitrogen use efficiency in salinized farmland in China, we summarized the effect of salinization on key processes of nitrogen transformation in farmland soil, analyzed the microbial mechanism underlying nitrogen transformation, and summed up the main ways for high efficient utilization of nitrogen in salinized farmland. Salinization had thre-shold effects on mineralization, nitrification, and denitrification of nitrogen from farmland soil, with the influence varying greatly in different scopes. Salinity and secondary barriers had different effects on microorganisms, with threshold in their effects. The most widely used methods for nitrogen synergism regulation in salinized farmland include soil conditioner, biomass material, growing salt-tole-rant plants, optimizing the ratio of different nitrogen forms, and biological inhibitor. We proposed current research shortcomings and future research directions of nitrogen cycle processes in salinized farmland. This study was of great significance for reducing nitrogen loss, enhancing utilization of nutrient from fertilizers, and controlling agricultural non-point source pollution in salinized farmland.


Assuntos
Nitrogênio , Solo , China , Fazendas , Nitrogênio/análise , Ciclo do Nitrogênio
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA