Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 612
Filtrar
1.
Chemosphere ; 239: 124612, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31499301

RESUMO

The degradation of carboxylic acid has been identified as one of the rate-determining steps in the mineralization of organic pollutants by ozonation. In this study, Ce(III)-doped graphitic carbon nitride (Ce-CN) composites with different Ce(III) contents were synthesized and used as catalysts for the ozonation of oxalate. The morphology and structure of the Ce-CN were comprehensively characterized using various techniques such as SEM, XRD, FTIR, and XPS. The results show that the structure of g-C3N4 provided an ideal site for the accommodation of Ce(III) and thus facilitated the formation of surface hydroxyl groups. With 2.5%Ce-CN as a catalyst, the degradation efficiency of oxalate was increased by 47.1% after reaction for 30 min. The decomposition of ozone was accelerated in the presence of Ce-CN. Hydroxyl radicals were recorded by electron spin resonance and identified as the major actives species. Under the catalysis of 2.5%Ce-CN, the production of hydroxyl radicals was increased by 40%. The Ce(III) and surface hydroxyl groups that distributed uniformly on the surface of Ce-CN were speculated as the dual catalytic sites for the complexation of oxalate and activation of ozone, respectively. Ce-CN had a high stability and reutilization capability. It is proposed that a complex was formed between surface Ce(III) and oxalate, and this complex could be more easily attacked by the surrounding ozone and hydroxyl radicals than free oxalate. As oxalate is a typical recalcitrant carboxylic acid, the findings from this study are expected to promote the application of ozonation in the removal of organic pollutants.

2.
Cancer Commun (Lond) ; 39(1): 75, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31730020

RESUMO

BACKGROUND: The National Comprehensive Cancer Network guidelines recommend intensity-modulated radiotherapy (IMRT) as the primary curative treatment for newly diagnosed nasopharyngeal carcinoma (NPC), but the radiation-related complications and relatively high medical costs remain a consequential burden for the patients. Endoscopic nasopharyngectomy (ENPG) was successfully applied in recurrent NPC with radiation free and relatively low medical costs. In this study, we examined whether ENPG could be an effective treatment for localized stage I NPC. METHODS: Ten newly diagnosed localized stage I NPC patients voluntarily received ENPG alone from June 2007 to September 2017 in Sun Yat-sen University Cancer Center. Simultaneously, the data of 329 stage I NPC patients treated with IMRT were collected and used as a reference cohort. The survival outcomes, quality of life (QOL), and medical costs between two groups were compared. RESULTS: After a median follow-up of 59.0 months (95% CI 53.4-64.6), no death, locoregional recurrence, or distant metastasis was observed in the 10 patients treated with ENPG. The 5-year overall survival, local relapse-free survival, regional relapse-free survival, and distant metastasis-free survival among the ENPG-treated patients was similar to that among the IMRT-treated patients (100% vs. 99.1%, 100% vs. 97.7%, 100% vs. 99.0%, 100% vs. 97.4%, respectively, P > 0.05). In addition, compared with IMRT, ENPG was associated with decreased total medical costs ($ 4090.42 ± 1502.65 vs. $ 12620.88 ± 4242.65, P < 0.001) and improved QOL scores including dry mouth (3.3 ± 10.5 vs. 34.4 ± 25.8, P < 0.001) and sticky saliva (3.3 ± 10.5 vs. 32.6 ± 23.3, P < 0.001). CONCLUSIONS: ENPG alone was associated with promising long-term survival outcomes, low medical costs, and satisfactory QOL and might therefore be an alternative strategy for treating newly diagnosed localized stage I NPC patients who refused radiotherapy. However, the application of ENPG should be prudent, and prospective clinical trials were needed to further verify the results.

3.
Opt Express ; 27(22): 31800-31809, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684405

RESUMO

We measure the transmission of near-infrared ps pulses through single CdTe nanowires. Benefitting from the strong light confinement and large effective nonlinearity of these nanowires, a significant spectral broadening of ∼ 5 nm and nonlinear phase shift of a few π due to self-phase modulation (SPM) is observed experimentally at coupled peak power of a dozen W with a propagating length down to several hundred µms. A nonlinear-index coefficient (n2) as high as (9.5 ± 1.4) × 10-17 m2/W at 1550 nm is extracted from transmission spectra, corresponding to a nonlinear parameter (γ) of ∼ 1050 W-1m-1. The simulations indicate a spectral broadening more than 1.5 µm in single nanowire when pumped by fs pulses in anomalous dispersion regime. The obtained results suggest that, CdTe nanowire is promising in developing ultracompact nonlinear optical devices for microphotonic circuits.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31714687

RESUMO

OBJECTIVE: To evaluate the feasibility, safety, and effectiveness of a novel, absorbable atrial septal defect (ASD) closure device made of poly-l-lactic acid (PLLA) in a swine model of ASD and for the first time in humans. METHODS: A preclinical safety study was conducted using a swine model of ASD. In a clinical setting, five pediatric patients underwent ASD closure with the PLLA device with fluoroscopic and transthoracic echocardiography guidance. The procedural results and clinical outcomes at 1 day, 30 days, 3 months, and 6 months after closure were analyzed. RESULTS: The 24- and 36-month follow-up results of the preclinical study demonstrated that the PLLA device exhibited good endothelialization and degradability in the swine model. In the clinical study, successful device implantation was achieved in all five patients (median age, 3.6 years; range, 3.1-6.5 years). The mean defect size was (13.6 ± 2.7) mm. Follow-up at 30 days, 3 months, and 6 months was completed in all five cases. The complete defect closure rates with no residual shunt at 30 days, 3 months, and 6 months follow-up were 60% (3/5), 80% (4/5), and 80% (4/5), respectively. No device dislodgement, significant aortic valve or mitral valve regurgitation, new onset cardiac arrhythmia, or other adverse events were reported. CONCLUSION: The study results demonstrated that it is feasible to implant the PLLA device for closure of small to medium sized ASDs without significant residual shunts or severe adverse events in humans. The PLLA device exhibited good endothelialization and degradability in the swine model at 24 and 36 months. Further studies to evaluate long-term safety and effectiveness with the device in a large cohort of patients are warranted.

5.
Anal Chim Acta ; 1092: 32-41, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31708030

RESUMO

Deer-hide gelatin (DHG) is an important animal-derived traditional Chinese medicine (TCM), which has been applied in TCM for over 400 years. However, it is extremely difficult to distinguish DHG with adulteration or made with other animal skins due to the highly processing procedure. Therefore, a simple strategy for identifying species-specific peptide biomarkers in deer-hide gelatin (DHG) is needed. In the present study, untargeted and targeted mass spectrometry approaches were implemented to analyze comprehensive peptidomic profiles of trypsin-digested animal gelatins. Mathematics set theory was then used to interrogate the relationship between different samples and peptides in the target species set, while the peptides were not considered as species-specific biomarkers in other sets. Two peptides were identified as DHG-specific peptides. Targeted mass spectrometry approach was then used to verify these two peptides. It showed that these two peptides could be used for distinguishing DHG from other animal hide gelatins. The present strategy provides a simple method for peptide biomarker discovery, which can be applied in the identification of specific peptides in some highly processed animal derived traditional Chinese medicines (TCMs). Thus, the present work provides an effective strategy for rapid, simple discovery and application of species-specific peptide biomarkers to ensure animal derived TCMs quality and make them authenticable and traceable.

6.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(8): 864-870, 2019 Aug 28.
Artigo em Chinês | MEDLINE | ID: mdl-31570672

RESUMO

OBJECTIVE: To explore roles of expressions of EHD2 and E-cadherin in clear cell renal cell carcinoma (ccRCC).
 Methods: Four couples of fresh ccRCC tissues and adjacent non-cancerous tissues were collected to evaluate the expression of EHD2 and E-cadherin protein by Western blotting. A total of 65 paraffin-embedded renal ccRCC tissues were collected, and immunohistochemical assay was used to detect the expression of EHD2 and E-cadherin in the samples. The correlation between their expression and clinical and pathological indicators of ccRCC was analyzed, and the relationship between EHD2 and E-cadherin proteins and prognosis for patients with ccRCC was also explored.
 Results: The results of Western blotting showed that the expression levels of EHD2 and E-cadherin were low in 4 ccRCC tissues compared with the adjacent noncancerous tissues. Immunohistochemical results revealed that the expressions of EHD2 and E-cadherin were higher in the localized ccRCC tissues than those in the metastatic ccRCC tissues; the expression levels of EHD2 and E-cadherin were decreased, while the TNM staging and Fuhrman grade were increased (P<0.05 or P<0.01). There was positive correlation between the expressions of EHD2 and E-cadherin in ccRCC (r=0.390, P<0.01). The progression-free survival in ccRCC patients with lower expression of both EHD2 and E-cadherin was better than that in ccRCC patients with higher expressions of EHD2 and E-cadherin (P<0.05). 
 Conclusion: The low expressions of EHD2 and E-cadherin are the potential indicators for the ccRCC patients with poor prognosis.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Caderinas , Proteínas de Transporte , Humanos , Estadiamento de Neoplasias , Prognóstico
7.
Parasit Vectors ; 12(1): 474, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31610804

RESUMO

BACKGROUND: The global spread of mosquito-borne diseases (MBD) has presented increasing challenges to public health. The transmission of MBD is mainly attributable to the biting behaviors of female mosquitoes. However, the ecological pattern of hourly host-seeking behavior in Aedes albopictus and its association with climatic variables are still not well understood, especially for a precise requirement for establishing an effective risk prediction system of MBD transmission. METHODS: Mosquito samples and data on mosquito hourly density and site-specific climatic variables, including temperature, relative humidity, illuminance and wind speed, were collected simultaneously in urban outdoor environments in Guangzhou during 2016-2018. Kernel regression models were used to assess the temporal patterns of hourly host-seeking behavior in mosquito populations, and negative binomial regression models in the Bayesian framework were used to investigate the associations of host-seeking behavior with climatic variables. RESULTS: Aedes albopictus was abundant, constituting 82% (5569/6790) of the total collected mosquitoes. Host-seeking behavior in Ae. albopictus varied across time and was significantly influenced by climatic variables. The predicted hourly mosquito densities showed non-linear relationships with temperature and illuminance, whereas density increased with relative humidity but generally decreased with wind speed. The range of temperature estimates for female biting was 16.4-37.1 °C, peaking at 26.5 °C (95% credible interval: 25.3-28.1). During the favorable periods, biting behavior of female Ae. albopictus was estimated to occur frequently all day long, presenting a bimodal distribution with peaks within 2-3 h around both dawn and dusk (05:00-08:00 h and 16:00-19:00 h). Moreover, a short-term association in hourly density between the females and males was found. CONCLUSIONS: Our field-based modeling study reveals that hourly host-seeking behavior of Ae. albopictus exhibits a complex pattern, with hourly variation constrained significantly by climatic variables. These findings lay a foundation for improving MBD risk assessments as well as practical strategies for vector control. For instances of all-day-long frequent female biting during the favorable periods in Guangzhou, effective integrated mosquito control measures must be taken throughout the day and night.

8.
Neural Netw ; 121: 484-496, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31648120

RESUMO

In multi-party deep learning, multiple participants jointly train a deep learning model through a central server to achieve common objectives without sharing their private data. Recently, a significant amount of progress has been made toward the privacy issue of this emerging multi-party deep learning paradigm. In this paper, we mainly focus on two problems in multi-party deep learning. The first problem is that most of the existing works are incapable of defending simultaneously against the attacks of honest-but-curious participants and an honest-but-curious server without a manager trusted by all participants. To tackle this problem, we design a privacy-enhanced multi-party deep learning framework, which integrates differential privacy and homomorphic encryption to prevent potential privacy leakage to other participants and a central server without requiring a manager that all participants trust. The other problem is that existing frameworks consume high total privacy budget when applying differential privacy for preserving privacy, which leads to a high risk of privacy leakage. In order to alleviate this problem, we propose three strategies for dynamically allocating privacy budget at each epoch to further enhance privacy guarantees without compromising the model utility. Moreover, it provides participants with an intuitive handle to strike a balance between the privacy level and the training efficiency by choosing different strategies. Both analytical and experimental evaluations demonstrate the promising performance of our proposed framework.

9.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(4): 500-505, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31642226

RESUMO

OBJECTIVE: To evaluate the expression of translocator protein (TSPO) in brain tissue within 72 h after subarachnoid hemorrhage (SAH) in mice. METHODS: Forty-four C57BL/6J mice were randomly divided into two groups, 17 in the Sham group and 27 in the SAH group. SAH mice model was performed by endovascular perforation as previously described with slight modifications. Sham group mice were performed by the same method but without piercing the blood vessels. Before and 6 h, 24 h, 48 h, 72 h after modeling, the two groups were scored with modified Garcia score for neurological function. At 6 h, 24 h, 48 h, 72 h after modeling, the mice were sacrificed. Sham group mice were sacrificed at 24 h after modeling. The expression of TSPO in brain tissue was evaluated by Western blot, positron emission tomography-computed tomography (PET-CT) and immunofluorescence staining. Fluorescent double staining was used to assess the relationship of TSPO and microglia. RESULTS: The neurological function scores of the SAH group mice decreased with time and then increased. The expression of TSPO in the brain tissue increased first and then decreased with time, and there was a negative correlation between them (r=-0.615 6, P < 0.01). PET-CT showed that the tracer intake of mouse brain tissue after SAH was higher than that of Sham group, and the difference was statistically significant (P < 0.05). Immunofluorescence staining showed that TSPO increased in the parietal cortex and basal cortex of the SAH group. And fluorescent double staining suggested that TSPO colocalized with Iba-1 which was a specific marker of microglia. CONCLUSIONS: In the early brain injury after SAH, the expression of TSPO in brain is widely increased, and the expression level increases first and then decreases. TSPO could participate in the activation of microglia and regulate the occurrence and development of brain injury after SAH.


Assuntos
Lesões Encefálicas/metabolismo , Microglia/metabolismo , Receptores de GABA/metabolismo , Hemorragia Subaracnóidea/metabolismo , Animais , Camundongos , Camundongos Endogâmicos C57BL , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Distribuição Aleatória
10.
Int J Surg ; 71: 182-189, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31610284

RESUMO

BACKGROUND: Diverticulitis is one of the most common gastrointestinal diseases in western population. Colonic resection is recommended by international guidelines as a routinely used technique for purulent diverticulitis. Laparoscopic lavage was introduced as a non-resection alternative. The studies available so far have shown contradictory results. This meta-analysis aims to compare laparoscopic lavage versus colonic resection in patients with Hinchey Ⅲ-Ⅳ diverticulitis. METHODS: We did a systematic review of articles published before March 20, 2019, with no language restriction by searching PubMed, Cochrane library, EMBASE databases, clinicaltrials.gov, and Google Scholar databases. We included all RCTs and cohort studies comparing outcomes between patients with Hinchey Ⅲ-Ⅳ diverticulitis undergoing laparoscopic lavage versus colonic resection. Important outcomes were mortality, complications, length of stay, readmission and reoperation rates. We combined data to assess the outcomes using DerSimonian and Laird random-effects model. RESULTS: A total of 569 patients with diverticulitis of which more than 80% were Hinchey Ⅲ were enrolled from 3 RCTs and 5 cohort studies. Laparoscopic lavage was associated with shorter operative time (WMD -78.9, 95%CI -100.58 to -57.11, P < 0.0001) and total postoperative hospital stay (WMD -7.62, 95%CI -11.60 to -3.63, P = 0.0002) but a higher rate of intra-abdominal abscess (OR 2.69, 95%CI 1.39 to 5.21, P = 0.0032) and secondary peritonitis (OR 5.30, 95%CI 1.91 to 14.73, P = 0.0014). CONCLUSION: Laparoscopic lavage for patients with Hinchey Ⅲ to Ⅳ diverticulitis does provide similar mortality, shorter operative time and hospital stay. However, the evidence so far suggests that it might be inadequate for sepsis control and may result in more unplanned reoperations. Further studies are needed to standardize the formal indication for laparoscopic lavage.

11.
Sheng Li Xue Bao ; 71(5): 769-782, 2019 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-31646331

RESUMO

Autoimmune diseases are a kind of chronic diseases with unclear etiology, which has the characteristics of repetition and difficulty to cure completely. Aerobic exercise, as an effective intervention method for chronic diseases, has also received extensive attention in the field of the prevention and treatment of autoimmune diseases. In this paper, the effects of aerobic exercise on immune system and autoimmune diseases in recent years are reviewed, and the related mechanisms are discussed. It is pointed out that aerobic exercise can improve the homeostasis of immune environment by affecting the number and function of immune cells, inhibit the systemic inflammatory response of the body, and then delay the occurrence and development of autoimmune diseases.


Assuntos
Doenças Autoimunes/prevenção & controle , Exercício , Sistema Imunitário , Homeostase , Humanos
12.
ACS Appl Mater Interfaces ; 11(43): 40677-40684, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31589402

RESUMO

Wearable electronics, such as smartwatches, VR (virtual reality)/AR (augmented reality) smartglasses, and E-textiles, are an emerging technology platform that is reshaping the way people interact with the surrounding world. However, the power source of these devices can be a critical issue, causing short operational/standby times and frequent charging. Here, a stretchable transparent wireless charging coil fabricated by negative adhesive transfer printing (NATP) is demonstrated. The stretchable transparent conductor is based on the silver nanowire (AgNW)-polyurethane acrylate (PUA) composite with high conductivity and robustness under harsh mechanical treatment. A 10.6 ohm/sq thin film has a transmittance of 84% and is still conductive under a mechanical deformation up to 60% tensile strain. A maximum power of 59 mW (power transfer efficiency ∼24%) is transferred wirelessly. A green-light-emitting diode (LED) was wirelessly powered to illustratively demonstrate the functionality of the system. This work provides an alternative power solution which is compatible with the soft and flexible components of wearable devices.

13.
Phys Rev Lett ; 123(9): 097001, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31524448

RESUMO

The recent theory-orientated discovery of record high-temperature superconductivity (T_{c}∼250 K) in sodalitelike clathrate LaH_{10} is an important advance toward room-temperature superconductors. Here, we identify an alternative clathrate structure in ternary Li_{2}MgH_{16} with a remarkably high estimated T_{c} of ∼473 K at 250 GPa, which may allow us to obtain room-temperature or even higher-temperature superconductivity. The ternary compound mimics a Li- or electron-doped binary hydride of MgH_{16}. The parent hydride contains H_{2} molecules and is not a good superconductor. The extra electrons introduced break up the H_{2} molecules, increasing the amount of atomic hydrogen compared with the parent hydride, which is necessary for stabilizing the clathrate structure or other high-T_{c} structures. Our results provide a viable strategy for tuning the superconductivity of hydrogen-rich hydrides by donating electrons to hydrides via metal doping. Our approach may pave the way for finding high-T_{c} superconductors in a variety of ternary or quaternary hydrides.

14.
Chin J Nat Med ; 17(7): 506-516, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31514982

RESUMO

MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil (CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain (MLC) phosphorylation. Meanwhile, the siRNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation, suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C. tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.

15.
Proc Natl Acad Sci U S A ; 116(39): 19392-19397, 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31501344

RESUMO

The Chinese Communist Revolution that culminated in the 1949 founding of the People's Republic of China fundamentally transformed class relations in China. With data from a nationally representative, longitudinal survey between 2010 and 2016, this study documents the long-term impact of the Communist Revolution on the social stratification order in today's China, more than 6 decades after the revolution. True to its stated ideological missions, the revolution resulted in promoting the social status of children of the peasant, worker, and revolutionary cadre classes and disadvantaging those who were from privileged classes at the time of the revolution. Although there was a tendency toward "reversion" mitigating the revolution's effects in the third generation toward the grandparents' generation in social status, the overall impact of reversion was small. The revolution effects were most pronounced for the birth cohorts immediately following the revolution, attenuating for recently born cohorts.

16.
Cell Mol Biol (Noisy-le-grand) ; 65(6): 44-51, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31472046

RESUMO

This study was carried out to investigate cell-free DNA (cfDNA) as a potential biomarker for colorectal cancer diagnosis.Patients with colorectal cancer (n = 25) who had not undergone surgery, and 35 patients with postoperative colorectal cancer were enrolled. Peripheral blood samples were collected from the colorectal cancer subjects (experimental group), and also from 30 healthy volunteers (control group). Quantitative PCR (qPCR) was used to determine cfDNA concentration and integrity in each group. The cfDNA levels of the two groups were analyzed to determine the relationship between the cfDNA and the clinical features of colorectal cancer patients. The receiver operator curve (ROC) was used to analyze sensitivity and specificity of cfDNA, carcinoembryonic antigen (CEA), cancer antigen 199 (CA199) and cancer antigen 125 (CA125). cfDNA concentration and cfDNA integrity in patients with colorectal cancer before surgery were significantly higher than those in patients with colorectal cancer after surgery, and cfDNA concentration of colorectal cancer patients after surgery was also significantly higher than that of the healthy control group, but the integrity was not significantly different from the control group. There was no significant correlation between cfDNA concentration/integrity and gender, age, disease stage, tumor location, tumor differentiation, and expressions of cancer antigen 153 (CA153), neuron specific enolase (NSE) and alpha fetoprotein (AFP) in colorectal cancer patients before or after surgery. However, there was a significant correlation between the expression levels of CEA/CA125 and concentration of cfDNA. The CA199 expression level was significantly correlated with cfDNA integrity. The sensitivity and specificity of cfDNA and integrity were higher than those used for traditional tumor biomarker detection. cfDNA concentration is significantly increased in serum of colorectal cancer patients. Thus, it may serve as a potential indicator of colorectal cancer.

17.
Cancer Res ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484669

RESUMO

The genetic events occuring in recurrent nasopharyngeal carcinoma (rNPC) are poorly understood. Here, we performed whole-genome and whole-exome sequencing in 55 rNPC and 44 primarily diagnosed NPC (pNPC) patients; with 7 patients having paired rNPC and pNPC samples. Previously published pNPC exome data were integrated for analysis. rNPC and pNPC tissues had similar mutational burdens, however, the number of clonal mutations was increased in rNPC samples. TP53 and three NF-κB pathway components (TRAF3, CYLD and NFKBIA) were significantly mutated in both pNPC and rNPC. Notably, mutations in TRAF3, CYLD and NFKBIA were all clonal in rNPC, however, 55.6-57.9% of them were clonal in pNPC. In general, the number of clonal mutations in NF-κB pathway-associated genes was significantly higher in rNPC than in pNPC. The NF-κB mutational clonality was selected and/or enriched during NPC recurrence. The amount of NF-κB translocated to the nucleus in samples with clonal NF-κB mutants was significantly higher than that in samples with subclonal NF-κB mutants. Moreover, the nuclear abundance of NF-κB protein was significantly greater in pNPC samples with local regional relapse than in those without relapse. Further, high nuclear NF-κB levels were an independent negative prognostic marker for locoregional relapse-free survival in pNPC. Lastly, inhibition of NF-κB enhanced both radiosensitivity and chemosensitivity in vitro and in vivo. In conclusion, NF-κB pathway activation by clonal mutations plays an important role in promoting the recurrence of NPC. Moreover, nuclear accumulation of NF-κB is a prominent biomarker for predicting locoregional relapse-free survival.

18.
Int J Neurosci ; : 1-9, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31516040

RESUMO

Purpose: Evidence has shown that propofol may cause widespread apoptotic neurodegeneration. Hypoxic preconditioning has been demonstrated to provide neuroprotection and brain recovery from both acute and chronic neurodegeneration in several cellular and animal models. However, the mechanism has not been well elucidated. Therefore, the present study was designed to investigate the expression of glucose transporters (GLUT1 and GLUT3) and mitochondrial division and fusion (Drp1 and Mfn2) proteins in rats exposed to hypoxic preconditioning to attenuate propofol neurotoxicity. Methods: Propofol (100 mg/kg) was given to 7-day-old Sprague-Dawley rats; in some rats, hypoxic preconditioning was administered before intraperitoneal propofol injection by subjecting rats to five cycles of 10 min of hypoxia (8% O2) and 10 min of normoxia (21% O2). Then, the rats were allowed to breathe room air for 2 h. Neuronal mitochondrial morphology was observed by transmission electron microscopy. ATP content was detected using an ATP assay kit. The expression levels of GLUT1, GLUT3, pDrp1, Drp1 and Mfn2 were detected by Western blot, and the expression levels of GLUT1 and GLUT3 were further examined by immunohistochemistry. Results: Propofol damaged mitochondria, and decreased ATP content and GLUT3 and pDrp1 protein expression. However, our results suggested that hypoxic preconditioning could attenuate propofol neurotoxicity by reducing mitochondrial damage and increasing ATP content and pDrp1, GLUT1 and GLUT3 protein expression. Conclusion: Hypoxic preconditioning reduced propofol-induced damage in the hippocampus of neonatal rats by attenuating the increase in mitochondrial division and decrease in GLUT3 expression.

19.
Chemistry ; 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31489999

RESUMO

A divergent strategy for the facile preparation of various enantioenriched phenylthio-substituted lactones was developed based on Lewis base/Brønsted acid co-catalyzed thiolation of homoallylic acids. The acid-controlled regiodivergent cyclization (6-endo vs. 5-exo) and acid-mediated stereoselective rearrangement of phenylthio-substituted lactones were explored. Experimental and computational studies were performed to clarify the origins of the regioselectivity and enantioselectivity. The calculation results suggest that C-O and C-S bond formation might occur simultaneously, without formation of a commonly supposed catalyst-coordinated thiiranium ion intermediate and the potential π-π stacking between substrate and SPh as an important factor in the enantio-determining step. Finally, this methodology was applied in the rapid syntheses of the bioactive natural products (+)-ricciocarpin A and (R)-dodecan-4-olide.

20.
Zhongguo Zhen Jiu ; 39(8): 889-95, 2019 Aug 12.
Artigo em Chinês | MEDLINE | ID: mdl-31397138

RESUMO

The internationally-accepted Consolidated Standards for Reporting of Trials (CONSORT) and Standards for Reporting Interventions in Controlled Trials of Acupuncture (STRICTA) were applied to evaluate the literature quality of randomized controlled trials (RCTs) regarding acupuncture for chronic neck pain in past 10 years. The literature of RCTs regarding acupuncture for chronic neck pain was searched by computer; the English literature was searched in PubMed and EMbase, while the Chinese literature was searched in CNKI, Wanfang database, VIP database and China Biomedical Literature Database. The literature published from January 2008 to January 2018 was searched. As a result, 29 Chinese articles and 10 English articles were included. According to CONSORT, among Chinese articles, 28 articles (96.6%) described baseline data, 23 articles (79.3%) described randomization, 0 articles (0.0%) described allocation concealment, 3 articles (10.3%) described blind method; among English articles, 6 articles (60.0%) described baseline data, 8 articles (80.0%) described randomization, 8 articles (80.0%) described allocation concealment, and 7 articles (70.0%) described blind method. According to STRICTA, among Chinese articles, 8 articles (27.6%) described needle instrument selection, 18 articles (62.1%) described needle depth, 24 articles (82.8%) described needling sensation, and 0 articles (0.0%) described acupuncturist' qualifications; among English articles, 5 articles (50.0%) described needle instrument selection, 8 articles (80.0%) described needle depth, 3 articles (30.0%) described needling sensation, and 4 articles (40.0%) described acupuncturist' qualifications. In conclusion, the reporting of acupuncture details in Chinese literature is superior to that in English literature, while the reporting of trial design in English literature is slightly superior to that in Chinese literature. Moreover, both Chinese and English literature need to further improve clinical trial design to improve the reporting quality of clinical evidence based on CONSORT and STRICTA.


Assuntos
Terapia por Acupuntura , Cervicalgia/terapia , China , Humanos , PubMed , Publicações , Ensaios Clínicos Controlados Aleatórios como Assunto
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA