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Sheng Wu Gong Cheng Xue Bao ; 29(12): 1836-46, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24660631


In order to study the effects of Ca2+ in the biosynthesis of salvianolic acid B (Sal B) induced by salicylic acid (SA) in the young seedlings of Salvia miltiorrhiza, we used confocal laser scanning microscopy and high performance liquid chromatography to measure the change of relative fluorescence intensity of Ca2+ and the contents of Sal B induced by SA before and after the application of extracellular calcium channel inhibitors (VP and LaCl3), intracellular calcium channel inhibitor (LiCl), as well as intracellular calmodulin antagonist (TFP). SA could induce the calcium burst, and the Ca2+ peak could last to 2-3 min in the guard cells of S. miltiorrhiza, which prompted the biosynthesis of Sal B after the Ca2+ burst. Both Vp or LaCl3, and LiCl or TFP could inhibit the burst of Ca2+ and the biosynthesis of Sal B. The above results demonstrated that Ca2+ from the extracellular and the intracellular calcium store regulate the biosynthesis of Sal B elicited by salicylic acid in S. miltiorrhiz young seedlings.

Benzofuranos/metabolismo , Cálcio/metabolismo , Ácido Salicílico/farmacologia , Salvia miltiorrhiza/metabolismo , Plântula/metabolismo , Folhas de Planta/metabolismo , Transdução de Sinais
Zhongguo Zhong Yao Za Zhi ; 38(20): 3424-31, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24490547


OBJECTIVE: To investigate the effect of intracellular and extracellular Ca2+ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. METHOD: Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid (SA), and then use the optimal concentration of SA to investigate the effects of extracellular Ca2+ channel inhibitors Verapamil, LaCl3, intracelluar calmodulin antagonist TFP and intracelluar Ca2+ channel inhibitors LiCl on the biosynthesis of RA and related enzymes. RESULT: SA increased the accumulation of RA and the activities of PAL and TAT, especially the SA of 2 mmol x L(-1) after 24 h. SA improved the accumulation of RA to (40.51 +/- 2.16) mg x g(-1), which was 1.97 times than that of control, and the activities of PAL, TAT were 1.42 times and 1.29 times than those of the control. However, Vp, LaCl3, TFP, LiCl inhibited the effects of SA evidently. CONCLUSION: Ca2+ plays a key role in the regulation of the induction process.

Cálcio/metabolismo , Cinamatos/metabolismo , Depsídeos/metabolismo , Ácido Salicílico/metabolismo , Salvia miltiorrhiza/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Salvia miltiorrhiza/genética , Salvia miltiorrhiza/crescimento & desenvolvimento , Plântula/genética , Plântula/crescimento & desenvolvimento , Plântula/metabolismo
Sheng Wu Gong Cheng Xue Bao ; 28(11): 1359-69, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23457788


We studied the influence of the concentration of Ca2+ (0-50 mmol/L) in culture medium on the synthesis of rosmarinic acid (RA) and related enzymes in Salvia miltiorrhiza suspension cultures. Using verpamil (VP, a calcium channel antagonist) and ionophore A23187, we studied the mechanism of secondary metabolites of Salvia miltiorrhiza suspension cultures influenced by the concentration of Ca2+ in the culture medium. The synthesis of intracellular RA in 6-day incubation was significantly dependent on the medium Ca2+ concentration. At the optimal Ca2+ concentration of 10 mmol/L, a maximal RA content of 20.149 mg/g biomass dry weight was reached, which was about 37.3% and 20.4% higher than that at Ca2+ concentrations of 1 and 3 mmol/L, respectively. The variation of the activity of PAL and TAT, two key enzymes of the two branches of RA, could be affected by the concentration of Ca2+ in culture medium. The change of their activity occurred prior to the accumulation of RA, which suggested both of the key enzymes be involved in the synthesis of RA. Meanwhile, the enzymatic action of PAL was more distinct than TAT. The treatment of VP and A23187, respectively, indicated that the influence of RA affected by the concentration of Ca2+ in the culture medium was accomplished by the intracellular Ca2+, and the flow of Ca2+ from the extracellular to the intracellular environment could also participate in this process.

Cálcio/farmacologia , Cinamatos/metabolismo , Depsídeos/metabolismo , Salvia miltiorrhiza/química , Salvia miltiorrhiza/enzimologia , Meios de Cultura , Técnicas de Cultura/métodos , Fenilalanina Amônia-Liase/metabolismo , Salvia miltiorrhiza/crescimento & desenvolvimento , Tirosina Transaminase/metabolismo