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1.
Ear Nose Throat J ; : 145561320923172, 2020 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-32425122

RESUMO

OBJECTIVE: To compare local anesthesia (LA) versus general anesthesia (GA) for balloon dilation of the Eustachian tube (BDET) using the Bielefeld Dilation System in a Chinese population with obstructive Eustachian tube dysfunction (OETD). METHODS: A total of 49 patients with OETD undergoing BDET under either LA or GA were included in the present study. Intraoperative blood pressure, heart rate, and pain sensation were recorded. The surgical outcome was assessed by Eustachian Tube Dysfunction Questionnaire-7 (ETDQ-7) symptom scores and positive Valsalva maneuver at baseline, 1 day, 2-, 4-, 12-, 24-, and 52-week follow-up. RESULTS: Balloon dilation of the Eustachian tube procedures were successfully completed in all patients without adverse effects. The visual analog scale score for maximal pain during the surgical procedure in the LA group was 6.1 ± 1.0. Intraoperative blood pressure and heart rate were higher in the LA group compared with the GA group. The duration and costs of surgery were significantly reduced in the LA group compared with the GA group. Normalization of ETDQ-7 scores at 12-week and 52-week follow-up was observed in 71.9% (23/32) and 63.3% (19/30) of patients in the LA group, respectively, which was comparable to that of the GA group. Although improvement in positive Valsalva maneuver was observed in both groups after BDET, more patients reported positive Valsalva maneuver in the GA group compared with the LA group at 12-week and 52-week follow-up. In total, 96.0% (24/25) of patients in the LA group and 95.8% (23/24) of patients in the GA group would choose LA if BDET was needed again. CONCLUSION: Balloon dilation of the Eustachian tube under LA is safe and feasible in a Chinese population. The surgical outcome of BDET is comparable between under LA and GA during 52-week follow-up. Further studies are needed to address management of intraoperative pain and determine longer follow-up outcome for BDET under LA.

2.
Toxicol Lett ; 2020 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-32428544

RESUMO

Cisplatin is a well-known and commonly used chemotherapeutic agent. However, cisplatin-induced ototoxicity limits its clinical use. Previous studies have shown an important role of reactive oxygen species (ROS) accumulation in the pathogenesis of cisplatin-induced ototoxicity. In many cell types, the transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2) and antioxidant response element (ARE) protect against oxidative stress by suppressing ROS. Here our results showed that cisplatin injury reduced Nrf2 expression and inhibited Nrf2 translocation in HEI-OC1 cells and Nrf2 activator tert-butylhydroquinone (TBHQ) rescued hair cells from cisplatin induced apoptosis by suppressing the total cellular ROS accumulation. Moreover, we found that decreased ROS accumulation induced by TBHQ didn't depend on mitochondrial derived ROS production, indicating that Nrf2 activation alleviated cisplatin induced oxidative stress and apoptosis through mitochondrial-independent ROS production. Therefore, we provide a potential strategy of prevention and treatment for cisplatin-induced ototoxicity by Nrf2 activation. In conclusion, Nrf2 activation protects auditory hair cells from cisplatin-induced ototoxicity through suppressing the total cellular ROS levels which arise from sources other than mitochondria.

3.
Biosci Rep ; 40(3)2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32154555

RESUMO

BACKGROUND: The present study investigated the effects of microvascular endothelial cells (MECs) on the chemotaxis, adhesion and proliferation of bone marrow hematopoietic stem cells (HSCs) ex vivo. METHODS AND RESULTS: MECs were collected from the lung tissue of C57BL/6 mice, and HSCs were isolated with immunomagnetic beads from bone marrow of GFP mice. MECs and HSCs were co-cultured with or without having direct cell-cell contact in Transwell device for the measurement of chemotaxis and adhesion of MECs to HSCs. Experimental results indicate that the penetration rate of HSCs from the Transwell upper chamber to lower chamber in 'co-culture' group was significantly higher than that of 'HSC single culture' group. Also, the HSCs in co-culture group were all adherent at 24 h, and the co-culture group with direct cell-cell contact had highest proliferation rate. The HSC number was positively correlated with vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels in supernatants of the culture. CONCLUSIONS: Our study reports that MECs enhance the chemotaxis, adhesion and proliferation of HSCs, which might be related to cytokines SDF-1 and VEGF secreted by MECs, and thus MECs enhance the HSC proliferation through cell-cell contact. The present study revealed the effect of MECs on HSCs, and provided a basis and direction for effective expansion of HSCs ex vivo.

4.
Invest New Drugs ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32189093

RESUMO

Losatuxizumab vedotin (formerly ABBV-221) is a second-generation antibody-drug conjugate targeting epidermal growth factor receptor (EGFR). In this multicenter phase 1 study, eligible patients with EGFR-dependent solid tumors received losatuxizumab vedotin (3 + 3 design) intravenously at starting dose of 0.3 mg/kg over 3 h per 21-day cycle, with alternate dosing schedules utilized (2 weeks on/1 week off or weekly) to mitigate infusion reactions. Forty-five patients received ≥1 doses of losatuxizumab vedotin (13 colon, 6 non-small cell lung cancer, 5 head and neck [HNC], 5 glioblastoma multiforme, 2 breast, 14 other). Tumor samples were evaluated for EGFR protein expression by immunohistochemistry, EGFR and EGFR ligand mRNA expression by RNAseq, and results compared with outcome. Most common adverse events were infusion-related reaction (22/45; 49%) and fatigue (20/45; 44%). While most infusion reactions were grade ≤ 2, four patients experienced grade ≥3 infusion reactions. Several infusion reaction mitigation strategies were explored. Because of the high incidence of infusion reactions, the trial was stopped and the maximum tolerated dose was not reached. The last cleared dose: 6 mg/kg/cycle. Nineteen patients (42%) had stable disease; 4 remained on study >6 months. One HNC patient with increased levels of EGFR and EGFR ligands (amphiregulin, epiregulin) achieved a confirmed partial response. Pharmacokinetic analysis of losatuxizumab vedotin showed exposures appeared to be approximately dose-proportional. The high frequency of infusion reactions necessitated early closure of this trial. The detailed mitigation strategies used in this protocol for infusion-related reactions may provide beneficial information for trial design of agents with high infusion reaction rates.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32203962

RESUMO

OBJECTIVE: To describe the feasibility of preserving the lesser occipital nerve (LON) and the great auricular nerve (GAN) in postauricular incision in ear surgery. METHODS: The distribution of the LON and the GAN was first identified in human cadavers. Then a clinical study was performed in 34 patients who underwent middle ear surgery between September 2016 and January 2017. Patients were divided into the conventional incision group and the modified incision group, according to incision types, and underwent sensory testing and subjective evaluation of auricular numbness after surgery at different times. RESULTS: Most frequently, the auricular branches of the LON went into the postauricular groove at the same height of inferior crus of antihelix. The vertical dimension from the intersection of the highest auricular branch of the GAN and postauricular groove to intertragic notch ranged from 5.7 to -4.2 mm. Preservation of the LON and the GAN reduced sensory loss in the modified incision group compared to the conventional incision group. CONCLUSION: Preservation of the LON and the GAN with modified postauricular incision can reduce postoperative auricular numbness.

6.
Nanoscale ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32016245

RESUMO

A range of power generating approaches, such as integration with clothing, fashion accessories, or textiles, allow electronic devices to be charged in environmentally friendly ways. Stretchable, efficient, stable, and even washable solar cells are considered the next necessary component to supply continuous wearable energy. However, ultra-thin photo-active materials are often fragile, which inevitably raises challenges for electron conduction during stretching and washing processes, thus resulting in unsatisfactory output performance. Herein, we have removed the stumbling block by designing a kirigami-inspired unique island-chain structure with serpentine interconnects, which prevented the photo-active layer of subcells from being subjected to excessive strain. Notably, this is the first time perovskite solar cell arrays met the above wearable requirements simultaneously. The obtained devices exhibited a high yet stable power output (efficiency of 17.68%) accompanied by a robust cycling performance (87% of the initial PCE) even after 300 times of continuous stretching with a large ratio of 80%. The efficiency of the optimized PSCs maintains promising stability after being exposed in a harsh environment (80% humidity) for 10 days. As textile-compatible power sources, the successfully designed stretchable and moisture-resistant photovoltaics add power-generation functionality to clothing, opening a new avenue for applications as long-term power sources for wearable electronics.

7.
Ear Nose Throat J ; : 145561320901398, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031003

RESUMO

BACKGROUND: Congenital auricular deformities are common diseases in newborn infants. We compared the efficacy of 2 ear molding systems in the nonsurgical management of newborn auricular deformities in Chinese infants. METHODS: A total of 462 newborns with auricular deformities were treated with either the EarWell or the LiangEar ear molding systems. The posttreatment outcome was graded as excellent, fair, and poor. The differences in effectiveness between the 2 ear molding systems and factors that may affect the posttreatment outcome were analyzed. RESULTS: Both ear molding systems showed substantial efficacy in the treatment of newborn auricular deformities. The effective rate was comparable between the EarWell and the LiangEar systems for 4 types of auricular deformities (cryptotia, prominent ear, helical rim abnormality, and cup ear), while the costs for the LiangEar systems were half as much as that for the EarWell systems. Multivariate regression analysis showed that earlier time points at treatment initiation, less severe auricular deformities, treatment duration, and breastfeeding were the most significant predictive factors for a better outcome. CONCLUSION: Our findings demonstrate that using the EarWell and the LiangEar systems are both optimal nonsurgical approaches for treatment of most newborn auricular deformities.

8.
Eur J Dermatol ; 30(1): 24-31, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32031529

RESUMO

BACKGROUND: Cutaneous adverse drug reactions (CADRs) are drug-induced skin reactions with or without systemic involvement, ranging from mild maculopapular exanthema (MPE) to life-threatening severe CADRs (S-CADRs). Due to their unpredictability and severity, early recognition of suspected causative drugs is highly recommended. However, the profile of CADRs remains unknown in China. OBJECTIVES: To assess the clinical profile, predominant causative drugs, and cost associated with CADRs in Shanghai, China. MATERIALS AND METHODS: Clinical records of inpatients admitted with a diagnosis of CADRs to the dermatology ward of Huashan Hospital from January 2007 to December 2016 were retrospectively studied. RESULTS: A total of 1,883 patients (1,231 female and 652 male), admitted with a diagnosis of CADR, were investigated. S-CADRs made up 21.99% of all cases (n=414), and urticaria (27.19%) was the most frequent reaction. Of the patients, 53.43% suffered from multiple drug-induced drug eruptions and the rest (45.83%) from single drug-induced drug eruptions. Overall, antimicrobials (28.85%) was the main drug group involved, and for S-CADRs, this was antiepileptic drugs (36.15%). The total cost for CADRs was RMB23,718,788.83 ($3,588,319.04). Both age and sex were related to admission cost (p=0.005 and p=7.84E-8, respectively). Antimicrobials were the most common treatment causing CADRs. CONCLUSION: The management of CADRs requires considerable medical cost. CADRs are not only a health problem but also a significant financial burden for affected individuals.

9.
Redox Biol ; 29: 101406, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31926629

RESUMO

The formation of reactive oxygen species (ROS) is a well-documented process in noise-induced hearing loss (NIHL). We have also previously shown that activation of 5' adenosine monophosphate (AMP)-activated protein kinase (AMPKα) at its catalytic residue T172 is one of the key reactions triggering noise-induced outer hair cell (OHC) death. In this study, we are addressing the link between ROS formation and activation of AMPKα in OHCs after noise exposure. In-vivo treatment of CBA/J mice with the antioxidant N-acetyl cysteine (NAC) reduced noise-induced ROS formation (as assessed by the relative levels of 4-hydroxynonenal and 3-nitrotyrosine) and activation of AMPKα in OHCs. Forskolin, an activator of adenylyl cyclase (AC) and an antioxidant, significantly increased cyclic adenosine monophosphate (cAMP) and decreased ROS formation and noise-induced activation of AMPKα. Consequently, treatment with forskolin attenuated noise-induced losses of OHCs and NIHL. In HEI-OC1 cells, H2O2-induced activation of AMPKα and cell death were inhibited by the application of forskolin. The sum of our data indicates that noise activates AMPKα in OHCs through formation of ROS and that noise-exposure-induced OHC death is mediated by a ROS/AMPKα-dependent pathway. Forskolin may serve as a potential compound for prevention of NIHL.

10.
Langmuir ; 36(3): 723-733, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31910022

RESUMO

Clays are prevalent in the earth's crust and usually deposited in the presence of water. An unusual finding in clays is that under certain conditions, water molecules can collectively form a bridge across a clay-hosted pore. However, there are relatively few studies focused on the formation mechanism of the water bridge in clay nanopores. In this work, we use molecular dynamics simulations to investigate the formation of the water bridge and its influence on fluid transport in slit-shaped illite nanopores. Two different basal illite surface chemistries are constructed: potassium-hydroxyl (P-H) and hydroxyl-hydroxyl (H-H) structures. Because pore size and water concentration are expected to control the formation of the water bridge, our simulations span a wide range of pore sizes and water concentrations. Generally, positive potassium layers and negative hydroxyl groups in P-H nanopore can induce partial charges which in return produce instant and local electric fields, favoring the formation of the water bridge. In P-H nanopores, the water bridge happens at a relatively low water concentration. However, in H-H nanopores, the water bridge only forms at high water concentrations. Additionally, smaller pore sizes favor the formation of water bridges. However, the presence of an electric field promotes the formation of a water bridge even in larger pore sizes in P-H pores. The results also indicate that in both P-H and H-H nanopores, water adsorption films initially create a smooth surface to promote the hydrocarbon flow. In P-H nanopores, further increases in the water concentration causes a sharp decline in the self-diffusion coefficients of the hydrocarbon and water due to the formation of the water bridge. The presence of electric fields in P-H pores can however weaken the confinement effect of illite and promote the hydrocarbon flow. In contrast, in H-H nanopores, the self-diffusion coefficients decline slowly with the increase of water concentration. This is because no water bridge is formed at low water concentrations in H-H nanopores.

11.
Nanoscale ; 12(3): 2118-2125, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31913389

RESUMO

Non-Hermitian physics basically due to the interplay between gain and loss has attracted considerable attention in the context of understanding various brand-new and counterintuitive physical phenomena. The major emphasis of this work is concerned with the chirality properties of chaotic motion in a whispering-gallery-mode microresonator based on nanoparticle-induced non-Hermitian mode coupling, which will be a challenging endeavor that is rarely presented in previous literature. By operating the nanoparticles in a whispering-gallery-mode microresonator, we achieved a dynamic control of chaotic behavior, and a rather more exotic finding is that the chaotic motion features chiral characteristics. Our results provide insight into nonlinear nano-optomechanics and fundamentally broaden the regime of chaotic dynamics. In addition, the proposal of chiral light chaos may offer attractive new prospects for the development of on-chip manipulation of chaotic light propagation and chiral photonic crystals, and could affect nanoscientific fields beyond optics.

12.
Adv Mater ; 32(4): e1906103, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31782558

RESUMO

Identifying the atomic structures of porous materials in spatial and temporal dimensions by (scanning) transmission electron microscope ((S)TEM) is significant for their wide applications in catalysis, separation and energy storage. However, the sensitivity of materials to electron beams made it difficult to reduce the electron damage to specimens while maintaining the resolution and signal-to-noise ratio. It is therefore still challenging to capture multiple images of the same area in one crystal to image the temporal changes of lattices. Usings integrated differential phase contrast (iDPC) STEM, atomic-resolution imaging of beam-sensitive zeolite frameworks is achieved with an ultralow dose of 40 e- Å-2 , 2-3 orders of magnitude lower than that of conventional STEM. Based on the iDPC technique, not only the atomic 3D architecture of ZSM-5 crystals but also the changes of frameworks are observed during in situ experiments. Local structures and light-element aromatics in ZSM-5 crystals can also be revealed directly under iDPC-STEM. These results provided not only an efficient tool to image beam-sensitive materials with ultralow beam current but also a new strategy to observe and investigate the hydrocarbon pools in zeolite catalysts at the single-molecule scale.

13.
Pharmacogenomics J ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31792370

RESUMO

Tanshinone, a widely used Chinese patent medicine, has been confirmed to have various kinds of pharmacological effects although frequently causing cutaneous adverse drug reactions (cADRs). We aim to identify whether human leukocyte antigen (HLA) class I alleles are associated with tanshinone-induced cADRs in Han Chinese. The association study including 18 patients with tanshinone-induced cADRs, 67 tanshinone-tolerant volunteers, and two general population databases consisted of 10,689 and 169,995 healthy subjects was performed. The frequency of tanshinone-induced cADRs patients carrying HLA-A*02:01 was significantly higher when compared with the general control groups (OR = 6.25, Pc = 7.20 × 10-5; OR = 7.14, Pc = 8.00 × 10-6), and with the tolerant group (OR = 5.09, Pc = 0.024). The molecular docking assay confirmed high affinity of the ingredients of tanshinone towards HLA-A*02:01 (≤-7.5 kcal/mol). The result suggested HLA-A*02:01 may work as a promisingly predictive marker for tanshinone personalized therapy in Han Chinese.

14.
Oxid Med Cell Longev ; 2019: 6150148, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31781342

RESUMO

Long noncoding RNA (lncRNA) disorder has been found in many kinds of age-associated diseases. However, the role of lncRNA in the development of age-related hearing loss (AHL) is still largely unknown. This study sought to uncover AHL-associated lncRNAs and the function. RNA-sequencing was conducted to profile lncRNA expression in the cochlea of an early-onset AHL mouse model. RT-qPCR assay was used to validate the expression pattern of lncRNAs. ATP assay, JC-1 assay, mitochondrial probe staining, CCK-8 assay, Western blot, and immunocytochemistry were performed to detect the effects of lncRNA AW112010 in HEI-OC1 cells and the mouse cochlea. We identified 88 significantly upregulated lncRNAs and 46 significantly downregulated lncRNAs in the cochlea of aged C57BL/6 mice. We focused on the significantly upregulated AW112010. Silencing of AW112010 decreased the ATP level, mitochondrial membrane potential, and cell viability and increased mitochondrial ROS generation under oxidative stress in HEI-OC1 cells. AW112010 overexpression promoted cell survival in HEI-OC1 cells. AW112010 knockdown reduced mitochondrial mass and impaired mitochondrial biogenesis in HEI-OC1 cells. Activation of mitochondrial biogenesis by resveratrol and STR1720 promoted cell survival. The mitochondrial biogenesis process was activated in the cochlea of aged mice. Moreover, AW112010 regulated AMPK signaling in HEI-OC1 cells. Transcription factor Arid5b elevated in the aged cochlea and induced AW112010 expression and mitochondrial biogenesis in HEI-OC1 cells. Taken together, lncRNAs are dysregulated with aging in the cochlea of C57BL/6 mice. The Arid5b/AW112010 signaling was induced in the aged mouse cochlea and positively modulated the mitochondrial biogenesis to maintain mitochondrial function.

15.
EBioMedicine ; 48: 92-99, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31594753

RESUMO

OBJECTIVE: To develop a deep convolutional neural network (DCNN) that can automatically detect laryngeal cancer (LCA) in laryngoscopic images. METHODS: A DCNN-based diagnostic system was constructed and trained using 13,721 laryngoscopic images of LCA, precancerous laryngeal lesions (PRELCA), benign laryngeal tumors (BLT) and normal tissues (NORM) from 2 tertiary hospitals in China, including 2293 from 206 LCA subjects, 1807 from 203 PRELCA subjects, 6448 from 774 BLT subjects and 3191 from 633 NORM subjects. An independent test set of 1176 laryngoscopic images from other 3 tertiary hospitals in China, including 132 from 44 LCA subjects, 129 from 43 PRELCA subjects, 504 from 168 BLT subjects and 411 from 137 NORM subjects, was applied to the constructed DCNN to evaluate its performance against experienced endoscopists. RESULTS: The DCCN achieved a sensitivity of 0.731, a specificity of 0.922, an AUC of 0.922, and the overall accuracy of 0.867 for detecting LCA and PRELCA among all lesions and normal tissues. When compared to human experts in an independent test set, the DCCN' s performance on detection of LCA and PRELCA achieved a sensitivity of 0.720, a specificity of 0.948, an AUC of 0.953, and the overall accuracy of 0.897, which was comparable to that of an experienced human expert with 10-20 years of work experience. Moreover, the overall accuracy of DCNN for detection of LCA was 0.773, which was also comparable to that of an experienced human expert with 10-20 years of work experience and exceeded the experts with less than 10 years of work experience. CONCLUSIONS: The DCNN has high sensitivity and specificity for automated detection of LCA and PRELCA from BLT and NORM in laryngoscopic images. This novel and effective approach facilitates earlier diagnosis of early LCA, resulting in improved clinical outcomes and reducing the burden of endoscopists.

16.
Opt Express ; 27(16): 22819-22826, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31510567

RESUMO

The discovery of topological photonic states has revolutionized our understanding of electromagnetic propagation and scattering. With the introduction of topology, some attractive properties such as unidirectional propagation and robustness against defects and impurities will be endowed to photonic edge modes. In this study, two-dimensionally confined topological edge states were achieved at terahertz (THz) frequency based on an all-dielectric photonic crystal structure. Trivial and nontrivial bandgaps of two deforming honeycomb lattices as well as unidirectional topological edge states were observed. Because the topological edge states with opposite helicity propagated in opposite directions at the interface, a polarization-resolved characteristic was demonstrated here, and thus a continuously tunable power splitter was achieved. This study provides some insights for further THz topological studies and possibilities for THz integrated platforms.

17.
Appl Opt ; 58(18): 4956-4962, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31503817

RESUMO

We report on the experimental observation of wavelength-switchable stretched pulse and bound-state pulse in a dispersion-managed Tm-doped laser. At a pump power of 572 mW, a stretched pulse with a pulse duration of 389 fs can be first obtained at 1961 nm. By increasing the pump power and appropriately adjusting the cavity polarization state, the mode-locking wavelength can be switched from 1961 nm to 1980 nm caused by the birefringence filtering effect based on nonlinear polarization rotation, and the corresponding pulse duration is 371 fs. Meanwhile, loosely bound states of two pulses and three pules at 1980 nm can be observed with appropriate cavity parameters.

18.
Dermatology ; : 1-13, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31522174

RESUMO

OBJECTIVE: Investigate the role of Yes-associated protein (YAP1) in the development of condyloma acuminatum (CA). METHODS: We enrolled 30 male patients with CA and 20 healthy individuals as a control group, to compare the YAP1 expression in their tissue samples. Following this, we overexpressed and downregulated YAP1 expression in HaCaT cells to examine the migratory, proliferative, and apoptotic potential of HaCaT cells expressing different levels of YAP1. RESULTS: In the CA patient tissue samples, an increase in YAP1 expression can be observed. In vitro,the overexpression of YAP1 was shown to promote the growth and migration of HaCaT cells and to activate epidermal growth factor receptor (EGFR) pathway-associated proteins, while the downregulation of YAP1 inhibited cell growth and migration of these cells. CONCLUSIONS: YAP1 promotes the growth of keratinocytes in CA through the activation of the EGFR pathway, and it may mediate the development of human papilloma virus-associated diseases.

19.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1053-1057, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418356

RESUMO

OBJECTIVE: To investigate the relationship of PTEN/PI3K/AKT signaling pathway protein expression with apoptosis and drug-resistance of children's ALL primary cells treated with daunorubicin (DNR). METHODS: The bone marrow mononuclear cells in newly diagnosed and untreated B-ALL children were collected and cultured. After the treatment of primary-cultured cells with DNR of final concentration 0.5 mg/L for 24 h, the cell apoptosis rate was detected by using cell apoptosis assay kit; the samples were collected at the on test of culture and after drug treatment, then expression levels of PTEN, PI3K and AKT proteins were detected by Western blot, moreover the interindex correlation was analyzed. RESULTS: After DNR treatment, the apoptosis rate in PTEN low expression group was lower than that in PTEN high expression group (P<0.05), showing high positive correlation of the cell apoptosis rate with the expression of PTEN before DNR treatment; the cell apoptosis rate in PI3K and AKT low expression group was higher than that in PI3K and AKT high expression group (P<0.01); however, the expression of PI3K and AKT proteins was down-regulated after treatment with DNR (P<0.01). CONCLUSION: The difference of PTEN expression is present in primary cells of B-ALL children, however the change of PTEN expression is not significant after DNR treatment, suggesting that the PTEN expression correlates with DNR-resistance. The DNR can induce the apoptosis of childrens B-ALL primary cells by down-regulating the expression of PI3K and AKT signaling pathway proteins.


Assuntos
Transdução de Sinais , Apoptose , Criança , Daunorrubicina , Humanos , PTEN Fosfo-Hidrolase , Fosfatidilinositol 3-Quinases , Proteínas Proto-Oncogênicas c-akt
20.
IEEE Int Conf Rehabil Robot ; 2019: 1173-1178, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31374788

RESUMO

Early fall detection is an important issue during gait rehabilitation training. This paper proposes an approach for pre-impact fall detection during gait rehabilitation training based on a 3D convolutional neural network (CNN). Firstly, pre-training data is collected and used to pre-train the 3D CNN to differentiate between a normal walking and a fall based on their general spatio-temporal patterns. Secondly, fine-tuning data is created by combining the pre-training data with a 3second normal walking sample collected from a new trainee whose falls are to be detected. The pre-trained 3D CNN is further fine-tuned by the fine-tuning data to learn the spatiotemporal patterns of the new trainee. Finally, a temporal sliding window is used to feed video snippets into the fine-tuned 3D CNN for fall detection. To the best of our knowledge, this is the first pre-impact fall detection approach based on a 3D CNN using RGB images. Moreover, the training strategy used to train the 3D CNN can alleviate the generalization issue of the 3D CNN when only limited training data is available in gait rehabilitation training. With 225 testing trials from 5 trainees, the proposed pre-impact fall detection approach achieves a detection accuracy of 100% within 0.5 second after falls start. Experiment results show that this approach is efficient, accurate, and practical in achieving intelligent fall detection during gait rehabilitation training.


Assuntos
Acidentes por Quedas/prevenção & controle , Algoritmos , Feminino , Marcha/fisiologia , Humanos , Masculino , Reabilitação
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